Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,411	A→G	intergenic (‑1059/‑172)	yjhQ ← / → yjhR	hypothetical protein/putative frameshift suppressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,411	0	A	G	87.0%	806.4 / 106.6	324	intergenic (‑1059/‑172)	yjhQ/yjhR	hypothetical protein/putative frameshift suppressor
Reads supporting (aligned to +/- strand): ref base A (26/16); new base G (126/155); total (152/172)
Fisher's exact test for biased strand distribution p-value = 4.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 327 total aligned reads displayed.

ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCA  >  NC_000913/4532320‑4532503

aCTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGGATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAg                                                                                             >  1:6217547/1‑93 (MQ=255)

 cTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCggg                                                                                            >  1:4753477/1‑93 (MQ=255)

  tGCCCACGATTAAAAGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGt                                                                                           >  2:6440212/1‑93 (MQ=255)

   gCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTa                                                                                          >  1:2549279/1‑93 (MQ=255)

     ccACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGc                                                                                        >  2:4417563/1‑93 (MQ=255)

      cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                       >  1:5230634/1‑93 (MQ=255)

       aCGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGccc                                                                                      >  2:4990429/1‑93 (MQ=255)

          aTTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGt                                                                                   <  2:2026215/93‑1 (MQ=255)

           ttAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGtt                                                                                  >  2:7058179/1‑93 (MQ=255)

           ttAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGtt                                                                                  >  1:4943022/1‑93 (MQ=255)

               aGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAc                                                                              >  2:5510442/1‑93 (MQ=255)

               aGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAc                                                                              >  2:1010003/1‑93 (MQ=255)

                    gCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCa                                                                         <  1:3051943/93‑1 (MQ=255)

                       gCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTa                                                                      >  1:2697111/1‑93 (MQ=255)

                       gCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTa                                                                      >  2:2286629/1‑93 (MQ=255)

                         tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAcc                                                                    >  1:6965588/1‑93 (MQ=255)

                         tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTAcc                                                                    >  2:1765586/1‑93 (MQ=255)

                         tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTAcc                                                                    >  1:3408248/1‑93 (MQ=255)

                          cAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCa                                                                   <  2:2715563/93‑1 (MQ=255)

                            ggCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGt                                                                 >  1:6894146/1‑93 (MQ=255)

                             gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                                <  1:4672686/93‑1 (MQ=255)

                             gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                                <  1:3931783/93‑1 (MQ=255)

                             gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                                >  2:3045708/1‑93 (MQ=255)

                             gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                                <  2:896666/93‑1 (MQ=255)

                              cGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAt                                                               <  2:4883390/93‑1 (MQ=255)

                              cGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAt                                                               <  1:1050072/93‑1 (MQ=255)

                               ggTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATg                                                              <  2:3259180/93‑1 (MQ=255)

                                gTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGa                                                             >  1:7438073/1‑93 (MQ=255)

                                gTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGa                                                             <  2:4927990/93‑1 (MQ=255)

                                    ccGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATccc                                                         <  2:5078128/93‑1 (MQ=255)

                                      gTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCaa                                                       >  1:3312147/1‑93 (MQ=255)

                                      gTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCaa                                                       <  1:2875481/93‑1 (MQ=255)

                                      gTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCaa                                                       <  1:2122923/93‑1 (MQ=255)

                                      gTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCaa                                                       <  1:1417250/93‑1 (MQ=255)

                                       tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                      >  1:1175426/1‑93 (MQ=255)

                                       tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                      <  1:7015548/93‑1 (MQ=255)

                                       tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                      >  1:4522102/1‑93 (MQ=255)

                                        aTTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGt                                                     >  2:332723/1‑93 (MQ=255)

                                         ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                    >  2:4466661/1‑93 (MQ=255)

                                         ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                    >  2:4174782/1‑93 (MQ=255)

                                          tCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTc                                                   >  1:4880598/1‑93 (MQ=255)

                                          tCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTc                                                   >  1:4201507/1‑93 (MQ=255)

                                            gATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGa                                                 <  2:3755359/93‑1 (MQ=255)

                                              taataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgc                                               <  2:3962677/93‑1 (MQ=255)

                                               aataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcg                                              >  1:5163318/1‑93 (MQ=255)

                                                ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                             <  1:131811/93‑1 (MQ=255)

                                                ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                             <  2:7388232/93‑1 (MQ=255)

                                                ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                             >  1:6712514/1‑93 (MQ=255)

                                                ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                             <  2:2380026/93‑1 (MQ=255)

                                                ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                             >  1:2426390/1‑93 (MQ=255)

                                                 taaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGcgcg                                            >  2:3832139/1‑93 (MQ=255)

                                                 taaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGcgcg                                            >  2:5338144/1‑93 (MQ=255)

                                                  aaaTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCgg                                           >  2:6087015/1‑93 (MQ=255)

                                                   aaTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCACAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGa                                          <  1:6615559/93‑1 (MQ=255)

                                                    aTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGaa                                         <  2:3868045/93‑1 (MQ=255)

                                                    aTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGaa                                         <  2:6172001/93‑1 (MQ=255)

                                                    aTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGaa                                         >  1:3136976/1‑93 (MQ=255)

                                                     tGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAt                                        <  1:6034235/93‑1 (MQ=255)

                                                     tGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAt                                        >  2:2781711/1‑93 (MQ=255)

                                                      gggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATg                                       <  1:5509416/93‑1 (MQ=255)

                                                      gggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATg                                       >  2:782009/1‑93 (MQ=255)

                                                       ggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGt                                      >  1:5742670/1‑93 (MQ=255)

                                                        gTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTa                                     <  2:7023809/93‑1 (MQ=255)

                                                        gTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTa                                     >  2:5413312/1‑93 (MQ=255)

                                                           tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                                  <  2:2574490/93‑1 (MQ=255)

                                                           tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                                  >  1:2975761/1‑93 (MQ=255)

                                                           tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                                  >  1:1871819/1‑93 (MQ=255)

                                                           tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTCCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                                  >  1:2248963/1‑93 (MQ=255)

                                                           tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                                  <  2:5845124/93‑1 (MQ=255)

                                                            ggAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTg                                 <  1:1738645/93‑1 (MQ=255)

                                                             gAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGt                                <  1:3628052/93‑1 (MQ=255)

                                                             gAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGt                                >  2:6291897/1‑93 (MQ=255)

                                                               aaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAt                              >  1:2031386/1‑93 (MQ=255)

                                                               aaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAt                              <  2:719528/93‑1 (MQ=255)

                                                                aaaGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATg                             >  1:5621223/1‑93 (MQ=255)

                                                                 aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                            <  2:478536/93‑1 (MQ=255)

                                                                 aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                            <  2:6940033/93‑1 (MQ=255)

                                                                 aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                            <  1:460045/93‑1 (MQ=255)

                                                                   gTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGc                          <  2:5138827/93‑1 (MQ=255)

                                                                     gcagcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAt                        >  1:2218092/1‑93 (MQ=255)

                                                                     gcagcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAt                        <  2:2598174/93‑1 (MQ=255)

                                                                      cagcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtc                       <  2:6648441/93‑1 (MQ=255)

                                                                        gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                     <  1:5079179/93‑1 (MQ=255)

                                                                        gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                     <  1:1615562/93‑1 (MQ=255)

                                                                         caTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtc                    <  2:6297785/93‑1 (MQ=255)

                                                                         caTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtc                    >  2:2056531/1‑93 (MQ=255)

                                                                         caTCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtc                    >  2:6032726/1‑93 (MQ=255)

                                                                          aTCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                   <  1:7219129/93‑1 (MQ=255)

                                                                            cTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCt                 <  1:6826648/93‑1 (MQ=255)

                                                                              ggCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGt               >  1:5324180/1‑93 (MQ=255)

                                                                               gCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGtt              <  1:4062768/93‑1 (MQ=255)

                                                                               gCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGtt              >  2:3666844/1‑93 (MQ=255)

                                                                                  gCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa           >  2:5744653/1‑93 (MQ=255)

                                                                                  gCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa           >  2:1310148/1‑93 (MQ=255)

                                                                                     tAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATa        >  1:2596217/1‑93 (MQ=255)

                                                                                     tAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATa        <  2:2916300/93‑1 (MQ=255)

                                                                                      aaTGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATaa       <  1:3702710/93‑1 (MQ=255)

                                                                                       aTGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAc      >  2:1566204/1‑93 (MQ=255)

                                                                                       aTGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAc      <  2:2543124/93‑1 (MQ=255)

                                                                                           aGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAcatca  <  2:4604274/93‑1 (MQ=255)

ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCA  >  NC_000913/4532320‑4532503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  NC_000913/4532318‑4532504

TAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCG                                                                                               <  SRR4302542.761230/94‑1 (MQ=60)

 AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGG                                                                                              >  SRR4302542.4662241/1‑94 (MQ=60)

 AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGG                                                                                              <  SRR4302542.7522891/94‑1 (MQ=60)

 AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGGATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAG                                                                                              >  SRR4302542.6278789/1‑94 (MQ=60)

  ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGG                                                                                             >  SRR4302542.4799781/1‑94 (MQ=60)

  ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGG                                                                                             <  SRR4302542.1719823/94‑1 (MQ=60)

   CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGT                                                                                            <  SRR4302542.7380138/94‑1 (MQ=60)

    TGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTA                                                                                           <  SRR4302542.2186482/94‑1 (MQ=60)

    TGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTA                                                                                           <  SRR4302542.6170408/94‑1 (MQ=60)

    TGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTA                                                                                           >  SRR4302542.2573981/1‑94 (MQ=60)

     GCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAG                                                                                          >  SRR4302542.6954024/1‑94 (MQ=60)

       CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302542.5281500/1‑94 (MQ=60)

        CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       >  SRR4302542.5467885/1‑94 (MQ=60)

          CGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAG                                                                                     >  SRR4302542.1489206/1‑94 (MQ=60)

          CGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAG                                                                                     <  SRR4302542.3824535/94‑1 (MQ=60)

           GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    >  SRR4302542.916895/1‑94 (MQ=60)

            ATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTT                                                                                   >  SRR4302542.4991052/1‑94 (MQ=60)

             TTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTC                                                                                  >  SRR4302542.4665128/1‑94 (MQ=60)

             TTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTC                                                                                  <  SRR4302542.6928627/94‑1 (MQ=60)

               AAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAA                                                                                >  SRR4302542.6995142/1‑94 (MQ=60)

                 AGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACT                                                                              <  SRR4302542.141369/94‑1 (MQ=60)

                   GTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCT                                                                            >  SRR4302542.2457886/1‑94 (MQ=60)

                    TGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           <  SRR4302542.4967485/94‑1 (MQ=60)

                     GGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCA                                                                          >  SRR4302542.1089419/1‑94 (MQ=60)

                      GCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCAC                                                                         >  SRR4302542.463675/1‑94 (MQ=60)

                      GCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCAC                                                                         <  SRR4302542.3081068/94‑1 (MQ=60)

                        CGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       >  SRR4302542.5192919/1‑94 (MQ=60)

                        CGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       >  SRR4302542.2723121/1‑94 (MQ=60)

                        CGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       <  SRR4302542.7246683/94‑1 (MQ=60)

                         GCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAC                                                                      >  SRR4302542.6784841/1‑94 (MQ=60)

                         GCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAC                                                                      >  SRR4302542.7091559/1‑94 (MQ=60)

                          CTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACC                                                                     >  SRR4302542.7034023/1‑94 (MQ=60)

                          CTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACC                                                                     >  SRR4302542.3440194/1‑94 (MQ=60)

                            CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   <  SRR4302542.2615229/94‑1 (MQ=60)

                            CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   <  SRR4302542.410358/94‑1 (MQ=60)

                             AGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGT                                                                  >  SRR4302542.6961847/1‑94 (MQ=60)

                              GGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTA                                                                 <  SRR4302542.536720/94‑1 (MQ=60)

                               GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302542.5192724/94‑1 (MQ=60)

                               GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302542.1102773/94‑1 (MQ=60)

                               GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302542.3970579/94‑1 (MQ=60)

                               GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302542.4718346/94‑1 (MQ=60)

                                CGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATG                                                               <  SRR4302542.1059109/94‑1 (MQ=60)

                                CGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATG                                                               >  SRR4302542.7061107/1‑94 (MQ=60)

                                 GGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGA                                                              >  SRR4302542.7510871/1‑94 (MQ=60)

                                  ggccccggATTCGGTAATAAATGGGGCTGGAAAAAGGGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCCCTACCAGTATGGT                                                             >  SRR4302542.3716335/9‑94 (MQ=60)

                                  GTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGAT                                                             <  SRR4302542.2994763/94‑1 (MQ=60)

                                    CACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCC                                                           <  SRR4302542.2027898/94‑1 (MQ=60)

                                    CACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCC                                                           >  SRR4302542.4722466/1‑94 (MQ=60)

                                     ACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCC                                                          <  SRR4302542.2338504/94‑1 (MQ=60)

                                     ACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCC                                                          >  SRR4302542.2750643/1‑94 (MQ=60)

                                      CCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCA                                                         <  SRR4302542.3649391/94‑1 (MQ=60)

                                      CCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCA                                                         <  SRR4302542.2059196/94‑1 (MQ=60)

                                       CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        >  SRR4302542.3343368/1‑94 (MQ=60)

                                       CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        >  SRR4302542.5492337/1‑94 (MQ=60)

                                       CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        <  SRR4302542.3176222/94‑1 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       <  SRR4302542.2903090/94‑1 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       <  SRR4302542.1429517/94‑1 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       >  SRR4302542.4566407/1‑94 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       <  SRR4302542.1386705/94‑1 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       <  SRR4302542.2141244/94‑1 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       >  SRR4302542.1185585/1‑94 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCCACTCTCACTACCAGTATGATCCCAAGT                                                      >  SRR4302542.5087057/1‑94 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      <  SRR4302542.7084452/94‑1 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      <  SRR4302542.4978550/94‑1 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      <  SRR4302542.252451/94‑1 (MQ=60)

                                          ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     >  SRR4302542.973896/1‑94 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    >  SRR4302542.4928093/1‑94 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    <  SRR4302542.2499292/94‑1 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    <  SRR4302542.4769986/94‑1 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    >  SRR4302542.4242930/1‑94 (MQ=60)

                                              GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 >  SRR4302542.1618503/1‑94 (MQ=60)

                                              GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 <  SRR4302542.5651982/94‑1 (MQ=60)

                                                TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302542.5213498/1‑94 (MQ=60)

                                                TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               <  SRR4302542.2258636/94‑1 (MQ=60)

                                                TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302542.2239214/1‑94 (MQ=60)

                                                TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               <  SRR4302542.3872183/94‑1 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              >  SRR4302542.6778634/1‑94 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              <  SRR4302542.6073222/94‑1 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              >  SRR4302542.6469136/1‑94 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              >  SRR4302542.2449988/1‑94 (MQ=60)

                                                  ATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCG                                             <  SRR4302542.3382346/94‑1 (MQ=60)

                                                  ATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCG                                             <  SRR4302542.132888/94‑1 (MQ=60)

                                                   TAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGC                                            <  SRR4302542.3086205/94‑1 (MQ=60)

                                                   TAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGCTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGG                                            <  SRR4302542.6273147/94‑1 (MQ=60)

                                                    AAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGA                                           >  SRR4302542.4306802/1‑94 (MQ=60)

                                                     AATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAA                                          <  SRR4302542.5001637/94‑1 (MQ=60)

                                                     AATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAA                                          >  SRR4302542.3166795/1‑94 (MQ=60)

                                                     AATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCACAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAA                                          <  SRR4302542.6680778/94‑1 (MQ=60)

                                                      ATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAT                                         >  SRR4302542.3110064/1‑94 (MQ=60)

                                                       TGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATG                                        <  SRR4302542.6092654/94‑1 (MQ=60)

                                                        GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       >  SRR4302542.5798426/1‑94 (MQ=60)

                                                        GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       <  SRR4302542.5562954/94‑1 (MQ=60)

                                                         GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTA                                      <  SRR4302542.4819540/94‑1 (MQ=60)

                                                          GTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTAT                                     <  SRR4302542.2983453/94‑1 (MQ=60)

                                                           TCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATC                                    <  SRR4302542.6783711/94‑1 (MQ=60)

                                                           TCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATC                                    <  SRR4302542.3260347/94‑1 (MQ=60)

                                                           TCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATC                                    <  SRR4302542.5231463/94‑1 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302542.3861915/1‑94 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302542.1888010/1‑94 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302542.3655445/94‑1 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302542.2872038/94‑1 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302542.3004258/1‑94 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302542.3708208/1‑94 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTCCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302542.2268303/1‑94 (MQ=60)

                                                             TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  <  SRR4302542.2049446/94‑1 (MQ=60)

                                                             TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  >  SRR4302542.5258680/1‑94 (MQ=60)

                                                             TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  >  SRR4302542.5676219/1‑94 (MQ=60)

                                                             TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  <  SRR4302542.443744/94‑1 (MQ=60)

                                                              GGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGT                                 <  SRR4302542.1753728/94‑1 (MQ=60)

                                                              GGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGT                                 >  SRR4302542.5249008/1‑94 (MQ=60)

                                                               GAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTA                                <  SRR4302542.3661851/94‑1 (MQ=60)

                                                                AAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAT                               <  SRR4302542.1775291/94‑1 (MQ=60)

                                                                AAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAT                               >  SRR4302542.2048960/1‑94 (MQ=60)

                                                                 AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302542.4067513/94‑1 (MQ=60)

                                                                 AAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              >  SRR4302542.5675830/1‑94 (MQ=60)

                                                                  AAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGA                             <  SRR4302542.3972763/94‑1 (MQ=60)

                                                                   AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302542.2728846/94‑1 (MQ=60)

                                                                   AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302542.3007851/94‑1 (MQ=60)

                                                                   AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302542.464024/94‑1 (MQ=60)

                                                                    AGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGC                           >  SRR4302542.3015984/1‑94 (MQ=60)

                                                                    AGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGC                           >  SRR4302542.5350646/1‑94 (MQ=60)

                                                                      TGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAT                         >  SRR4302542.2237174/1‑94 (MQ=60)

                                                                        CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       <  SRR4302542.5642346/94‑1 (MQ=60)

                                                                        CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       <  SRR4302542.4896328/94‑1 (MQ=60)

                                                                        CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       >  SRR4302542.2659881/1‑94 (MQ=60)

                                                                         AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      >  SRR4302542.6715285/1‑94 (MQ=60)

                                                                         AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      <  SRR4302542.1711827/94‑1 (MQ=60)

                                                                         AGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      >  SRR4302542.7473551/1‑94 (MQ=60)

                                                                          GCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTC                     <  SRR4302542.1629518/94‑1 (MQ=60)

                                                                          GCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTC                     <  SRR4302542.5699951/94‑1 (MQ=60)

                                                                          GCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTC                     <  SRR4302542.5128541/94‑1 (MQ=60)

                                                                           CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    <  SRR4302542.3381748/94‑1 (MQ=60)

                                                                            ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   >  SRR4302542.1609469/1‑94 (MQ=60)

                                                                            ATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   <  SRR4302542.7289945/94‑1 (MQ=60)

                                                                             TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302542.2025964/94‑1 (MQ=60)

                                                                              CTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTG                 <  SRR4302542.6893744/94‑1 (MQ=60)

                                                                              CTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTG                 >  SRR4302542.3339397/1‑94 (MQ=60)

                                                                              CTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTG                 >  SRR4302542.1211065/1‑94 (MQ=60)

                                                                               TGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGT                >  SRR4302542.5375905/1‑94 (MQ=60)

                                                                                 GCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTT              <  SRR4302542.4102833/94‑1 (MQ=60)

                                                                                 GCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTT              <  SRR4302542.171663/94‑1 (MQ=60)

                                                                                  CAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTC             >  SRR4302542.7063737/1‑94 (MQ=60)

                                                                                  CAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTC             <  SRR4302542.243781/94‑1 (MQ=60)

                                                                                   AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            <  SRR4302542.7014745/94‑1 (MQ=60)

                                                                                   AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            >  SRR4302542.2220807/1‑94 (MQ=60)

                                                                                   AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            >  SRR4302542.4708794/1‑94 (MQ=60)

                                                                                   AGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            >  SRR4302542.4180046/1‑94 (MQ=60)

                                                                                    GCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAA           <  SRR4302542.4592473/94‑1 (MQ=60)

                                                                                     CGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAAT          >  SRR4302542.1304150/1‑94 (MQ=60)

                                                                                      GTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         >  SRR4302542.6340750/1‑94 (MQ=60)

                                                                                      GTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         <  SRR4302542.6975747/94‑1 (MQ=60)

                                                                                      GTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         >  SRR4302542.2621350/1‑94 (MQ=60)

                                                                                      GTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         <  SRR4302542.1838740/94‑1 (MQ=60)

                                                                                      GTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         >  SRR4302542.6721167/1‑94 (MQ=60)

                                                                                       TAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAA        <  SRR4302542.2595750/94‑1 (MQ=60)

                                                                                       TAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAA        <  SRR4302542.2720003/94‑1 (MQ=60)

                                                                                       TAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAA        <  SRR4302542.5728805/94‑1 (MQ=60)

                                                                                        AATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAC       <  SRR4302542.4258719/94‑1 (MQ=60)

                                                                                        AATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAC       <  SRR4302542.4889830/94‑1 (MQ=60)

                                                                                        AATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAC       <  SRR4302542.3739351/94‑1 (MQ=60)

                                                                                         ATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA      <  SRR4302542.3665858/94‑1 (MQ=60)

                                                                                         ATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA      >  SRR4302542.211134/1‑94 (MQ=60)

                                                                                          TGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACAT     <  SRR4302542.6025143/94‑1 (MQ=60)

                                                                                          TGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACAT     <  SRR4302542.6396233/94‑1 (MQ=60)

                                                                                            CGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCA   <  SRR4302542.3640705/94‑1 (MQ=60)

                                                                                            CGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCA   <  SRR4302542.2358867/94‑1 (MQ=60)

                                                                                             GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  SRR4302542.306102/1‑94 (MQ=60)

                                                                                             AGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  SRR4302542.363737/1‑94 (MQ=60)

TAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  NC_000913/4532318‑4532504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`