Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,593,254 2 bp→GC coding (59‑60/807 nt) yjjM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,2540CG100.0% 859.7 / NA 252A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (127/125);  total (127/125)
*NC_0009134,593,2550GC100.0% 852.2 / NA 252A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (127/125);  total (127/125)

Only 100 of 252 total aligned reads displayed.

ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGAAGT  >  NC_000913/4593167‑4593340
                                                                                       ||                                                                                     
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 >  1:3636000/1‑93 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 >  1:1091158/1‑93 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 >  2:1064477/1‑93 (MQ=255)
   cATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                <  1:1622363/93‑1 (MQ=255)
     ttGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcgc                                                                              >  2:7505618/1‑93 (MQ=255)
        aaGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTa                                                                           <  2:5812341/93‑1 (MQ=255)
          gCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAg                                                                         <  2:7462340/93‑1 (MQ=255)
           cGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGt                                                                        <  1:4974461/93‑1 (MQ=255)
            ggTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTg                                                                       >  1:5964093/1‑93 (MQ=255)
             gtagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTgg                                                                      <  1:2855710/93‑1 (MQ=255)
              tagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGc                                                                     >  1:4559132/1‑93 (MQ=255)
              tagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGc                                                                     >  2:184021/1‑93 (MQ=255)
               agtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCt                                                                    <  1:5479325/93‑1 (MQ=255)
               agtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCt                                                                    <  2:5965650/93‑1 (MQ=255)
                 taCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTga                                                                  >  1:5607355/1‑93 (MQ=255)
                 taCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTga                                                                  >  2:6768980/1‑93 (MQ=255)
                  aCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgag                                                                 <  1:5412013/93‑1 (MQ=255)
                   cAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgaga                                                                >  1:7312194/1‑93 (MQ=255)
                   cAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgaga                                                                >  1:605307/1‑93 (MQ=255)
                   cAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgaga                                                                >  2:1423008/1‑93 (MQ=255)
                   cAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgaga                                                                >  1:88141/1‑93 (MQ=255)
                    aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGaa                                                               <  1:5724597/93‑1 (MQ=255)
                    aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGaa                                                               >  1:5342352/1‑93 (MQ=255)
                       cGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                                                            <  1:2955718/93‑1 (MQ=255)
                        gAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATg                                                           <  1:2657531/93‑1 (MQ=255)
                        gAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATg                                                           <  2:2165243/93‑1 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          <  1:3456265/93‑1 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          <  2:1485349/93‑1 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          >  2:5055951/1‑93 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          >  2:1653714/1‑93 (MQ=255)
                          aaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                         >  1:2630626/1‑93 (MQ=255)
                            ccGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGc                                                       >  1:6741278/1‑93 (MQ=255)
                               tcatcaTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACt                                                    >  1:4014730/1‑93 (MQ=255)
                               tcatcaTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACt                                                    <  2:4221111/93‑1 (MQ=255)
                               tcatcaTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACt                                                    >  2:6317942/1‑93 (MQ=255)
                               tcatcaTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACt                                                    >  2:904170/1‑93 (MQ=255)
                                catcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                   <  2:3145885/93‑1 (MQ=255)
                                catcatGGTCAGGTTTACGCGCAATAACATAGTCGCTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                   <  2:6462614/93‑1 (MQ=255)
                                 atcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                  >  2:377332/1‑93 (MQ=255)
                                 atcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                  >  1:6124200/1‑93 (MQ=255)
                                 atcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                  <  2:62286/93‑1 (MQ=255)
                                  tcatGGTCAGGTTTACGCGCAGTAACATAGTCGTTTCCAACCTGCGTCAAGGCGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                 <  2:6600807/93‑1 (MQ=255)
                                  tcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                 >  2:347590/1‑93 (MQ=255)
                                  tcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                 >  1:6425158/1‑93 (MQ=255)
                                   catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtgg                                                >  1:4778733/1‑93 (MQ=255)
                                   catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtgg                                                <  2:3944942/93‑1 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              >  1:4150090/1‑93 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              <  1:3977045/93‑1 (MQ=255)
                                      ggTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                             >  1:7448070/1‑93 (MQ=255)
                                      ggTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                             <  1:13565/93‑1 (MQ=255)
                                      ggTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                             <  1:3169375/93‑1 (MQ=255)
                                       gTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCg                                            >  2:2500347/1‑93 (MQ=255)
                                            gTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGCCTGAGAATATGACGCACTGTGGTGCGGCTGa                                       <  1:4488985/93‑1 (MQ=255)
                                             tttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                      <  1:7201043/93‑1 (MQ=255)
                                             tttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                      >  1:1384256/1‑93 (MQ=255)
                                             tttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                      <  2:1160013/93‑1 (MQ=255)
                                               tACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgt                                    <  2:5538199/93‑1 (MQ=255)
                                               tACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgt                                    <  1:3055014/93‑1 (MQ=255)
                                               tACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgt                                    >  1:3776724/1‑93 (MQ=255)
                                                aCGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgtt                                   <  2:7211684/93‑1 (MQ=255)
                                                aCGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgtt                                   >  1:2421629/1‑93 (MQ=255)
                                                aCGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgnn                                   >  1:1463287/1‑91 (MQ=255)
                                                 cgcgCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttg                                  <  1:7348271/93‑1 (MQ=255)
                                                  gcgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttgt                                 <  1:936396/93‑1 (MQ=255)
                                                   cgcAATAACATAGTTGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTa                                >  2:4054221/1‑93 (MQ=255)
                                                   cgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTa                                <  2:427855/93‑1 (MQ=255)
                                                    gcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAc                               >  2:5796912/1‑93 (MQ=255)
                                                      aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAt                             <  2:3522609/93‑1 (MQ=255)
                                                      aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAt                             >  1:5887526/1‑93 (MQ=255)
                                                       aTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAtt                            >  2:4609264/1‑93 (MQ=255)
                                                        tAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACCttt                           <  1:6974105/93‑1 (MQ=255)
                                                         aaCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTc                          <  1:1145318/93‑1 (MQ=255)
                                                          aCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTcc                         <  2:4944938/93‑1 (MQ=255)
                                                          aCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTcc                         <  2:614237/93‑1 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  1:5674251/1‑93 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  2:3346694/1‑93 (MQ=255)
                                                            aTAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGc                       >  2:2587406/1‑93 (MQ=255)
                                                            aTAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGc                       >  1:6256067/1‑93 (MQ=255)
                                                                tCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgc                   >  2:3092464/1‑93 (MQ=255)
                                                                   tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCa                <  1:2790835/93‑1 (MQ=255)
                                                                   tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCa                >  1:1904858/1‑93 (MQ=255)
                                                                   tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCa                >  1:292703/1‑93 (MQ=255)
                                                                   tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCa                >  1:3576555/1‑93 (MQ=255)
                                                                    ttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAc               <  2:5608493/93‑1 (MQ=255)
                                                                     tCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt              <  2:1235486/93‑1 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             >  2:3680754/1‑93 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             >  1:23896/1‑93 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             >  1:5196714/1‑93 (MQ=255)
                                                                       cAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTTCGCCAGCGCACttt            >  2:2844790/1‑93 (MQ=255)
                                                                       cAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACttt            >  2:2092056/1‑93 (MQ=255)
                                                                       cAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACttt            <  1:2104469/93‑1 (MQ=255)
                                                                        aaCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTg           >  2:2765558/1‑93 (MQ=255)
                                                                        aaCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTg           >  2:4459253/1‑93 (MQ=255)
                                                                         aCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGc          >  2:450749/1‑93 (MQ=255)
                                                                            tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAt       <  1:2996645/93‑1 (MQ=255)
                                                                             gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATg      <  2:4869460/93‑1 (MQ=255)
                                                                              cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgg     <  1:6557104/93‑2 (MQ=255)
                                                                               gTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgga    <  2:5354993/93‑3 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTCCATTTCCGCCAGCGCACTTTGCGATggaag  <  2:4757389/93‑5 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  1:6224201/93‑5 (MQ=255)
                                                                                       ||                                                                                     
ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGAAGT  >  NC_000913/4593167‑4593340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGG  >  NC_000913/4593161‑4593342
                                                                                             |                                                                                         
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                           >  SRR4302542.1468174/1‑94 (MQ=60)
     CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                      >  SRR4302542.3727560/1‑94 (MQ=60)
     CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                      >  SRR4302542.6805506/1‑94 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                     <  SRR4302542.3314549/94‑1 (MQ=60)
       CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                    >  SRR4302542.1100508/1‑94 (MQ=60)
       CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                    >  SRR4302542.3669908/1‑94 (MQ=60)
        TCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                   <  SRR4302542.2899311/94‑1 (MQ=60)
        TCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                   <  SRR4302542.3610542/94‑1 (MQ=60)
         CATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCG                                                                                  <  SRR4302542.1636375/94‑1 (MQ=60)
          ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGC                                                                                 >  SRR4302542.3503393/1‑94 (MQ=60)
            TGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                               >  SRR4302542.4067311/1‑94 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                          >  SRR4302542.6021834/1‑94 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                          <  SRR4302542.5022754/94‑1 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                          <  SRR4302542.149126/94‑1 (MQ=60)
                  GGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGG                                                                         <  SRR4302542.944309/94‑1 (MQ=60)
                   GTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                        >  SRR4302542.4603777/1‑94 (MQ=60)
                   GTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                        >  SRR4302542.2820221/1‑94 (MQ=60)
                   GTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                        <  SRR4302542.7419930/94‑1 (MQ=60)
                   GTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                        <  SRR4302542.2883152/94‑1 (MQ=60)
                     AGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTG                                                                      <  SRR4302542.5532548/94‑1 (MQ=60)
                     AGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTG                                                                      <  SRR4302542.3011173/94‑1 (MQ=60)
                      GTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGA                                                                     >  SRR4302542.5661831/1‑94 (MQ=60)
                       TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAG                                                                    <  SRR4302542.5831100/94‑1 (MQ=60)
                       TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAG                                                                    <  SRR4302542.3013682/94‑1 (MQ=60)
                       TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAG                                                                    <  SRR4302542.1915603/94‑1 (MQ=60)
                        ACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGA                                                                   >  SRR4302542.88875/1‑94 (MQ=60)
                        ACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGA                                                                   >  SRR4302542.610518/1‑94 (MQ=60)
                        ACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGA                                                                   <  SRR4302542.5464577/94‑1 (MQ=60)
                        ACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGA                                                                   >  SRR4302542.7383816/1‑94 (MQ=60)
                         CAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAA                                                                  >  SRR4302542.5394252/1‑94 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 <  SRR4302542.5295003/94‑1 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 <  SRR4302542.5780164/94‑1 (MQ=60)
                           GGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                >  SRR4302542.4991424/1‑94 (MQ=60)
                            GCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATAT                                                               <  SRR4302542.546498/94‑1 (MQ=60)
                             CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATG                                                              >  SRR4302542.5676417/1‑94 (MQ=60)
                             CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATG                                                              >  SRR4302542.596450/1‑94 (MQ=60)
                             CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATG                                                              <  SRR4302542.2984007/94‑1 (MQ=60)
                             CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATG                                                              <  SRR4302542.2967716/94‑1 (MQ=60)
                              GAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGA                                                             <  SRR4302542.2683185/94‑1 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                            >  SRR4302542.2656042/1‑94 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                            <  SRR4302542.3488598/94‑1 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                           <  SRR4302542.5370289/94‑1 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                           >  SRR4302542.3525881/1‑94 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                           <  SRR4302542.540617/94‑1 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                           <  SRR4302542.3745466/94‑1 (MQ=60)
                                 ACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGC                                                          >  SRR4302542.1204544/1‑94 (MQ=60)
                                 ACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGC                                                          >  SRR4302542.6807625/1‑94 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                        <  SRR4302542.7178930/94‑1 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                        >  SRR4302542.6877554/1‑94 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                       <  SRR4302542.96837/94‑1 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                       >  SRR4302542.4054359/1‑94 (MQ=60)
                                      CATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGT                                                     >  SRR4302542.6183457/1‑94 (MQ=60)
                                       ATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                    <  SRR4302542.1416987/94‑1 (MQ=60)
                                       ATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                    >  SRR4302542.6488474/1‑94 (MQ=60)
                                        TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                   >  SRR4302542.4825317/1‑94 (MQ=60)
                                        TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                   <  SRR4302542.4626581/94‑1 (MQ=60)
                                        TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                   <  SRR4302542.3055118/94‑1 (MQ=60)
                                        TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                   <  SRR4302542.1432180/94‑1 (MQ=60)
                                         CATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGT                                                  <  SRR4302542.5694218/94‑1 (MQ=60)
                                         CATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGT                                                  <  SRR4302542.219676/94‑1 (MQ=60)
                                          ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                 <  SRR4302542.492000/94‑1 (MQ=60)
                                          ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                 >  SRR4302542.4190964/1‑94 (MQ=60)
                                          ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                 >  SRR4302542.7399195/1‑94 (MQ=60)
                                           TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                >  SRR4302542.7520994/1‑94 (MQ=60)
                                           TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                <  SRR4302542.4016286/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                               <  SRR4302542.7341691/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                               <  SRR4302542.7466059/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                               <  SRR4302542.13678/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                               <  SRR4302542.3199440/94‑1 (MQ=60)
                                               CAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCT                                            <  SRR4302542.3104258/94‑1 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302542.2279422/1‑94 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302542.898820/1‑94 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302542.2667524/1‑94 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302542.6194694/1‑94 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302542.1371985/1‑94 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302542.2912966/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                         <  SRR4302542.1104026/94‑1 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                         >  SRR4302542.2792203/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                         >  SRR4302542.1396215/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGCCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                         <  SRR4302542.4533035/94‑1 (MQ=60)
                                                   TTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATG                                        >  SRR4302542.5849215/1‑94 (MQ=60)
                                                   TTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATG                                        <  SRR4302542.7271710/94‑1 (MQ=60)
                                                    TTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGT                                       >  SRR4302542.3814091/1‑94 (MQ=60)
                                                    TTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGT                                       >  SRR4302542.6214533/1‑94 (MQ=60)
                                                     TACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTT                                      <  SRR4302542.4046152/94‑1 (MQ=60)
                                                     TACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTT                                      <  SRR4302542.3084163/94‑1 (MQ=60)
                                                     TACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTT                                      >  SRR4302542.2445173/1‑94 (MQ=60)
                                                     TACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGNN                                      >  SRR4302542.1475918/1‑94 (MQ=60)
                                                       CGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGT                                    <  SRR4302542.7420239/94‑1 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                   <  SRR4302542.944463/94‑1 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                   <  SRR4302542.6087326/94‑1 (MQ=60)
                                                         CGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAC                                  <  SRR4302542.5513585/94‑1 (MQ=60)
                                                           CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                <  SRR4302542.2430278/94‑1 (MQ=60)
                                                           CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                <  SRR4302542.2071260/94‑1 (MQ=60)
                                                           CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                >  SRR4302542.5944586/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                              <  SRR4302542.3882993/94‑1 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                              >  SRR4302542.6545293/1‑94 (MQ=60)
                                                              TAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACCTTTC                             <  SRR4302542.7042611/94‑1 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                            <  SRR4302542.2601463/94‑1 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                            <  SRR4302542.2464761/94‑1 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                            >  SRR4302542.7450132/1‑94 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                            <  SRR4302542.1155131/94‑1 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                            <  SRR4302542.789184/94‑1 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                           >  SRR4302542.5729354/1‑94 (MQ=60)
                                                                 CATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGC                          >  SRR4302542.6317708/1‑94 (MQ=60)
                                                                  ATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCC                         >  SRR4302542.4053896/1‑94 (MQ=60)
                                                                  ATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCC                         >  SRR4302542.4071614/1‑94 (MQ=60)
                                                                   TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCA                        <  SRR4302542.6310960/94‑1 (MQ=60)
                                                                     GTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGC                      <  SRR4302542.3133476/94‑1 (MQ=60)
                                                                      TCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCG                     >  SRR4302542.5207156/1‑94 (MQ=60)
                                                                        GTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCA                   >  SRR4302542.3609932/1‑94 (MQ=60)
                                                                        GTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCA                   >  SRR4302542.295256/1‑94 (MQ=60)
                                                                        GTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCA                   >  SRR4302542.1921319/1‑94 (MQ=60)
                                                                         TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAC                  <  SRR4302542.2817698/94‑1 (MQ=60)
                                                                          TTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACT                 >  SRR4302542.1265647/1‑94 (MQ=60)
                                                                           TCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTT                >  SRR4302542.24101/1‑94 (MQ=60)
                                                                           TCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTT                >  SRR4302542.5247207/1‑94 (MQ=60)
                                                                            CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT               <  SRR4302542.6375663/94‑1 (MQ=60)
                                                                            CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT               >  SRR4302542.6439635/1‑94 (MQ=60)
                                                                             CAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG              <  SRR4302542.2122629/94‑1 (MQ=60)
                                                                              AACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC             >  SRR4302542.7215712/1‑94 (MQ=60)
                                                                              AACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC             <  SRR4302542.2751917/94‑1 (MQ=60)
                                                                              AACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC             >  SRR4302542.574493/1‑94 (MQ=60)
                                                                                CCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA           >  SRR4302542.6057187/1‑94 (MQ=60)
                                                                                 CTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT          >  SRR4302542.6697430/1‑94 (MQ=60)
                                                                                 CTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT          <  SRR4302542.6179593/94‑1 (MQ=60)
                                                                                  TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G        >  SRR4302542.4571738/1‑94 (MQ=60)
                                                                                  TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G        <  SRR4302542.1657741/94‑1 (MQ=60)
                                                                                  TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G        <  SRR4302542.3025321/94‑1 (MQ=60)
                                                                                   GCGTCAAGACGCCGCATTCACGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG       >  SRR4302542.5619955/1‑94 (MQ=60)
                                                                                    CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GGA      <  SRR4302542.6621738/94‑1 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa     >  SRR4302542.4576499/1‑91 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa     >  SRR4302542.4196658/1‑91 (MQ=60)
                                                                                      TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAG     <  SRR4302542.3437004/94‑1 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGT    <  SRR4302542.6285497/94‑1 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGT    >  SRR4302542.1219149/1‑94 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGT    >  SRR4302542.2539204/1‑94 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG   >  SRR4302542.5967259/1‑94 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG   >  SRR4302542.2712035/1‑94 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG   <  SRR4302542.530609/94‑1 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG  <  SRR4302542.6022251/94‑1 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG  <  SRR4302542.2827060/94‑1 (MQ=60)
                                                                                             |                                                                                         
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGG  >  NC_000913/4593161‑4593342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑