Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,898 Δ1 bp intergenic (+6/‑85) yjjG → / → prfC putative phosphatase/peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,8980T.79.9% 638.2 / 236.2 277intergenic (+6/‑85)yjjG/prfCputative phosphatase/peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base T (26/24);  new base . (77/122);  total (111/166)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 285 total aligned reads displayed.

CCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGA  >  NC_000913/4606808‑4606986
                                                                                          |                                                                                        
ccATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCcccc                                                                                        >  1:367282/1‑93 (MQ=255)
  aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTcccc                                                                                      >  2:2692602/1‑93 (MQ=255)
   tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGcccccccc                                                                                     >  2:1132506/1‑93 (MQ=255)
       gAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGtt                                                                                <  1:6425340/93‑1 (MQ=255)
       gAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGtt                                                                                <  1:7178925/93‑1 (MQ=255)
         gCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTg                                                                               >  2:5787617/1‑93 (MQ=255)
         gCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGa                                                                              <  1:1412380/93‑1 (MQ=255)
         gCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGa                                                                              <  2:1377203/93‑1 (MQ=255)
          cAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGAt                                                                             <  1:1477158/93‑1 (MQ=255)
            aCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATg                                                                            >  1:6032320/1‑93 (MQ=255)
            aCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATg                                                                            >  1:6761831/1‑93 (MQ=255)
            aCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATgg                                                                           <  1:4021617/93‑1 (MQ=255)
             ccAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATggg                                                                          <  1:1555018/93‑1 (MQ=255)
              cAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATggg                                                                          <  1:749788/93‑1 (MQ=255)
              cAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGC‑‑CCCCCCCGTTGATGGGTa                                                                        <  1:235829/93‑1 (MQ=255)
               aGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTa                                                                        >  2:5428758/1‑93 (MQ=255)
                gAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTaa                                                                       <  2:297227/93‑1 (MQ=255)
                  aGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTaaa                                                                      >  1:4909966/1‑93 (MQ=255)
                  aGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTaaaa                                                                     <  2:3263962/93‑1 (MQ=255)
                   ggCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAAt                                                                    <  2:2519741/93‑1 (MQ=255)
                     cATCGCGCCCACCTGGACCGTTTCTTGGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAg                                                                  <  2:737053/93‑1 (MQ=255)
                      aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGc                                                                 <  2:4923615/93‑1 (MQ=255)
                      aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGc                                                                 >  2:447668/1‑93 (MQ=255)
                       tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGcc                                                                <  2:1840658/93‑1 (MQ=255)
                            cccACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCaa                                                            <  1:1950634/93‑1 (MQ=255)
                             ccACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAAtt                                                          >  2:7039273/1‑93 (MQ=255)
                             ccACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGAAGGGTAAAATAGCCGCAAtt                                                          <  2:5478862/93‑1 (MQ=255)
                              cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCCCCCCCCCCGTTTTGGGTGAAAAAACCGccaat                                                          >  2:1273366/1‑89 (MQ=255)
                              cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAAtttt                                                        >  2:6829127/1‑93 (MQ=255)
                               cccTGGGCCCTTTCTTCGTTTGCCGAACCGGGGCCGCCTCCGTGTAAAACCTGATTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAAttt                                                         <  1:3724657/92‑1 (MQ=255)
                               aCCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAAtttt                                                        >  2:908877/1‑93 (MQ=255)
                                 cTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTc                                                      <  2:6278008/93‑1 (MQ=255)
                                  tGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCg                                                     >  1:3104798/1‑93 (MQ=255)
                                   ggACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCg                                                     <  1:1649202/93‑1 (MQ=255)
                                   ggACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGGAAAATAGCCGCAATTTTTTGt                                                    >  1:2639894/1‑93 (MQ=255)
                                    gACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGt                                                    >  2:191847/1‑93 (MQ=255)
                                    gACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtt                                                   <  2:310894/93‑1 (MQ=255)
                                    gACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtt                                                   <  1:4406078/93‑1 (MQ=255)
                                    gACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATCGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtt                                                   <  1:5122707/93‑1 (MQ=255)
                                     aCCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGttt                                                  >  2:3397634/1‑93 (MQ=255)
                                      ccGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGttt                                                  <  1:81369/93‑1 (MQ=255)
                                      ccGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtttt                                                 <  1:919205/93‑1 (MQ=255)
                                       cGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtttt                                                 >  2:1732309/1‑93 (MQ=255)
                                       cGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTc                                                <  2:4015124/93‑1 (MQ=255)
                                       cGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTc                                                >  1:5034021/1‑93 (MQ=255)
                                       cGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTca                                               <  2:7082078/93‑1 (MQ=255)
                                        gTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTc                                                <  1:5420510/93‑1 (MQ=255)
                                        gTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTca                                               <  2:6074616/93‑1 (MQ=255)
                                        gTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGGAAAATAGCCGCAATTTTTTGttttta                                               >  1:3889659/1‑91 (MQ=255)
                                         tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaa                                              >  1:6536598/1‑93 (MQ=255)
                                          ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac                                             >  1:1070735/1‑93 (MQ=255)
                                           tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaaca                                            <  2:812297/93‑1 (MQ=255)
                                            cttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaacaa                                           <  2:5090986/93‑1 (MQ=255)
                                             ttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCCCCCCCCCCTTGATGGGTAAAATAGCCCCAATTTTTCGTTTTtaaaaa                                           >  2:3179583/1‑93 (MQ=255)
                                             ttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAg                                          <  1:3232655/93‑1 (MQ=255)
                                             ttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgc                                         >  1:2764087/1‑93 (MQ=255)
                                              tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgc                                         <  2:5220922/93‑1 (MQ=255)
                                              tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgc                                         <  1:4358917/93‑1 (MQ=255)
                                              tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgc                                         <  1:705104/93‑1 (MQ=255)
                                               cGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                        >  1:5563931/1‑93 (MQ=255)
                                                gTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc                                       <  1:3451715/93‑1 (MQ=255)
                                                gTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc                                       <  2:3526222/93‑1 (MQ=255)
                                                   gCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggc                                    <  2:213826/93‑1 (MQ=255)
                                                   gCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggc                                    <  2:4321971/93‑1 (MQ=255)
                                                       gAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGa                                <  2:4710441/93‑1 (MQ=255)
                                                       gAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGa                                >  2:3711519/1‑93 (MQ=255)
                                                        aaCTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                               <  1:452663/93‑1 (MQ=255)
                                                        aaCTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                               <  2:3634954/93‑1 (MQ=255)
                                                          cTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGc                             <  2:5684536/93‑1 (MQ=255)
                                                          cTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGc                             <  2:2443171/93‑1 (MQ=255)
                                                              agcagcTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                         <  1:3923382/93‑1 (MQ=255)
                                                              agcagcTCATGTGTAAACACTGATTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGc                          <  2:166169/93‑1 (MQ=255)
                                                                 agcTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTAc                      <  2:5962085/93‑1 (MQ=255)
                                                                  gcTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACt                     >  1:5860334/1‑93 (MQ=255)
                                                                   cTCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCttcatc                    >  1:7094198/1‑89 (MQ=255)
                                                                    tCCTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCa                   <  2:4158940/93‑1 (MQ=255)
                                                                     ccTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCa                   <  1:4639817/93‑1 (MQ=255)
                                                                     ccTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa                  <  2:1402197/93‑1 (MQ=255)
                                                                     ccTGTGTAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa                  <  2:6748870/93‑1 (MQ=255)
                                                                       tgtgTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaag                 <  2:440669/93‑1 (MQ=255)
                                                                        gtgtAAACACTGATTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaaga                <  2:4212025/93‑1 (MQ=255)
                                                                        gtgtAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaagaa               <  2:2842599/93‑1 (MQ=255)
                                                                        gtgtAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaagaa               <  1:4785245/93‑1 (MQ=255)
                                                                          gtAAACACTGATTGCC‑CCCCCCCGTTGATGGGGAAAATAGCCGCAATTTTTTGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTTAagaaga             >  2:2549496/1‑93 (MQ=255)
                                                                           tAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga             <  2:6252139/93‑1 (MQ=255)
                                                                           tAAACACTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa            >  2:7236319/1‑93 (MQ=255)
                                                                              acacTGATTGCC‑CCCCCTCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaaga         <  2:6491310/93‑1 (MQ=255)
                                                                                 cTGATTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAAt       <  1:1000412/93‑1 (MQ=255)
                                                                                 cTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAAtt      <  2:6621327/93‑1 (MQ=255)
                                                                                 cTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAAtt      <  1:1101684/93‑1 (MQ=255)
                                                                                 cTGATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAAtt      >  2:750652/1‑93 (MQ=255)
                                                                                 cTGATT‑CCCCCCCCCCGTTGATAGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAAtt      <  1:6339059/93‑1 (MQ=255)
                                                                                  tGATTGCC‑CCCCCCCGTTGATGAGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTa     <  1:54000/93‑1 (MQ=255)
                                                                                   taTTGCCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTa     <  1:3560202/92‑1 (MQ=255)
                                                                                   gATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTa     <  2:3931869/93‑1 (MQ=255)
                                                                                   gATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAt    >  2:6445156/1‑93 (MQ=255)
                                                                                   gATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAt    <  2:5564930/93‑1 (MQ=255)
                                                                                   gATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAt    <  1:7400294/93‑1 (MQ=255)
                                                                                   gATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAt    <  2:4354535/93‑1 (MQ=255)
                                                                                   gATTGCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAt    <  2:4890469/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGA  >  NC_000913/4606808‑4606986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTG  >  NC_000913/4606805‑4606991
                                                                                              |                                                                                              
ACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC                                                                                                >  SRR4302542.6835982/1‑94 (MQ=60)
 CACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑C                                                                                              >  SRR4302542.2436946/1‑94 (MQ=60)
  ACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CC                                                                                             >  SRR4302542.1011132/1‑94 (MQ=60)
  ACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CC                                                                                             >  SRR4302542.370474/1‑94 (MQ=60)
  ACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CC                                                                                             >  SRR4302542.3781486/1‑94 (MQ=60)
  ACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CC                                                                                             <  SRR4302542.3273909/94‑1 (MQ=60)
    CATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCC                                                                                           >  SRR4302542.5503094/1‑94 (MQ=60)
         CGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTT                                                                                     >  SRR4302542.3773062/1‑94 (MQ=60)
         CGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTT                                                                                     >  SRR4302542.7159472/1‑94 (MQ=60)
          GAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTG                                                                                    <  SRR4302542.6488657/94‑1 (MQ=60)
          GAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTG                                                                                    <  SRR4302542.7249339/94‑1 (MQ=60)
           AGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGA                                                                                   <  SRR4302542.1756622/94‑1 (MQ=60)
            GCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGAT                                                                                  <  SRR4302542.1424588/94‑1 (MQ=60)
            GCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGAT                                                                                  >  SRR4302542.6083557/1‑94 (MQ=60)
             CAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATG                                                                                 <  SRR4302542.1489901/94‑1 (MQ=60)
              AACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATG                                                                                 >  SRR4302542.6828344/1‑94 (MQ=60)
              AACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATG                                                                                 >  SRR4302542.6090722/1‑94 (MQ=60)
               ACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCGTTGATGG                                                                                <  SRR4302542.368608/94‑1 (MQ=60)
               ACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGG                                                                               <  SRR4302542.4061301/94‑1 (MQ=60)
                CCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGT                                                                              >  SRR4302542.5460975/1‑94 (MQ=60)
                CCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGT                                                                              <  SRR4302542.1568439/94‑1 (MQ=60)
                 CAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGT                                                                              <  SRR4302542.756272/94‑1 (MQ=60)
                 CAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAA                                                                            <  SRR4302542.237857/94‑1 (MQ=60)
                  AGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTTCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCGTTGATGGGTA                                                                             <  SRR4302542.2959045/94‑1 (MQ=60)
                  AGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAA                                                                            >  SRR4302542.4843602/1‑94 (MQ=60)
                   GAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCttggtggggaaa                                                                            >  SRR4302542.4649792/1‑82 (MQ=60)
                    AAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAA                                                                           >  SRR4302542.3151320/1‑94 (MQ=60)
                    AAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAA                                                                           >  SRR4302542.4957720/1‑94 (MQ=60)
                    AAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAAAAT                                                                         <  SRR4302542.5075022/94‑1 (MQ=60)
                     AGGCATCCCGCCCCCCTGGACCGTTTCTTCGTTCCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAAT                                                                         <  SRR4302542.3758474/94‑1 (MQ=60)
                      GGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATA                                                                        <  SRR4302542.7236777/94‑1 (MQ=60)
                      GGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAAAATAG                                                                       <  SRR4302542.3157959/94‑1 (MQ=60)
                         ATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGC                                                                      >  SRR4302542.3493248/1‑94 (MQ=60)
                             CGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGGGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGCAAAAAAGCCGCAA                                                                 >  SRR4302542.5227220/1‑94 (MQ=60)
                               CCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAAT                                                                <  SRR4302542.1967504/94‑1 (MQ=60)
                                CCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTT                                                              >  SRR4302542.5452599/1‑94 (MQ=60)
                                  ccctgggccctttcttcgtttgccgaaccggggccgcctccgtgtaaaacC‑TGATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTT                                                             <  SRR4302542.3761541/44‑1 (MQ=60)
                                    CTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCG                                                          >  SRR4302542.3134307/1‑94 (MQ=60)
                                    CTGGACCGTTTCTTCGCTCCACGAACTGGATCAACTCCTGTATAAACTC‑TTATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCG                                                          <  SRR4302542.4511194/94‑1 (MQ=59)
                                     TGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGGAAAATAGCCGCAATTTTTTGT                                                         >  SRR4302542.2665389/1‑94 (MQ=60)
                                     TGG‑CCGTTTTTTCGTTGCACGAACTGGGGCAGCTCCTTTTTAAACACTTGTTTCCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCG                                                          <  SRR4302542.7049308/94‑1 (MQ=50)
                                      GGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGT                                                         <  SRR4302542.1663456/94‑1 (MQ=60)
                                      GGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTT                                                        >  SRR4302542.917875/1‑94 (MQ=60)
                                      GGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGGAAAATAGCCGCCATTTTTCTTT                                                        >  SRR4302542.2491817/1‑94 (MQ=60)
                                       GACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTT                                                       <  SRR4302542.4449350/94‑1 (MQ=60)
                                       GACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTT                                                       <  SRR4302542.5766954/94‑1 (MQ=60)
                                       GACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATCGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTT                                                       <  SRR4302542.5172450/94‑1 (MQ=60)
                                        ACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCgcttgttggggaaaaaaaccgcaatttttttttt                                                       >  SRR4302542.2503732/1‑60 (MQ=60)
                                        ACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTT                                                      >  SRR4302542.3637459/1‑94 (MQ=60)
                                        ACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTC                                                     <  SRR4302542.3724566/94‑1 (MQ=60)
                                         CCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTT                                                      <  SRR4302542.82049/94‑1 (MQ=60)
                                         CCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTC                                                     >  SRR4302542.5082887/1‑94 (MQ=60)
                                         CCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTC                                                     <  SRR4302542.927126/94‑1 (MQ=60)
                                          CGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCCTTGGTGGGTAAAATAGCCGCAATTTTTCGTTTTC                                                     >  SRR4302542.7542164/1‑94 (MQ=60)
                                          CGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGGAAAATAGCCGCAATTTTTTGTTTTTA                                                    >  SRR4302542.3928065/1‑94 (MQ=60)
                                           GTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCA                                                    <  SRR4302542.5473141/94‑1 (MQ=60)
                                           GTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAA                                                   >  SRR4302542.6601017/1‑94 (MQ=60)
                                            TTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAAC                                                  >  SRR4302542.1079934/1‑94 (MQ=60)
                                            TTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAAC                                                  >  SRR4302542.4154715/1‑94 (MQ=60)
                                             TTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACA                                                 >  SRR4302542.62626/1‑94 (MQ=60)
                                               TTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAG                                               <  SRR4302542.7226918/94‑1 (MQ=60)
                                               CTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGC                                              >  SRR4302542.2790724/1‑94 (MQ=60)
                                                TTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGC                                              <  SRR4302542.3263225/94‑1 (MQ=60)
                                                 TCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCG                                             <  SRR4302542.4401718/94‑1 (MQ=60)
                                                 TCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCG                                             >  SRR4302542.5617994/1‑94 (MQ=60)
                                                 TCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCG                                             <  SRR4302542.711207/94‑1 (MQ=60)
                                                  CGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGC                                              <  SRR4302542.4992511/94‑1 (MQ=60)
                                                  CGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGC                                            >  SRR4302542.574361/1‑94 (MQ=60)
                                                   GTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCG                                           <  SRR4302542.3484005/94‑1 (MQ=60)
                                                    tttacagaaccggagcaaacCCTGTTTAAAAAC‑TGATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCG                                           <  SRR4302542.3886521/74‑1 (MQ=60)
                                                     TGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCG                                        <  SRR4302542.1470236/94‑1 (MQ=60)
                                                      GCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCNGCG                                        <  SRR4302542.207862/94‑1 (MQ=60)
                                                      GCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCG                                        <  SRR4302542.4428438/94‑1 (MQ=60)
                                                      GCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCG                                        <  SRR4302542.782171/94‑1 (MQ=60)
                                                       CACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGAGCGGCGC                                       >  SRR4302542.422268/1‑94 (MQ=60)
                                                           AACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATG                                   <  SRR4302542.456570/94‑1 (MQ=60)
                                                           AACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATG                                   >  SRR4302542.7576701/1‑94 (MQ=60)
                                                            ACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATG                                   <  SRR4302542.3932437/94‑1 (MQ=60)
                                                              TGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCG                                >  SRR4302542.6522996/1‑94 (MQ=60)
                                                              TGGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCG                                >  SRR4302542.6343164/1‑94 (MQ=60)
                                                               GGAGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTtgtggggaaaaaaaccccaattttttgtttttaaaaaacgccgggcgctggcgc                               >  SRR4302542.2657502/1‑40 (MQ=60)
                                                                 AGCAGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTT                             <  SRR4302542.3962094/94‑1 (MQ=60)
                                                                 AGCAGCTCCTGTGTAAACAC‑TGATTGC‑‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTA                            <  SRR4302542.5092882/94‑1 (MQ=60)
                                                                  GCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTT                             <  SRR4302542.3953684/94‑1 (MQ=60)
                                                                    AGCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACT                          >  SRR4302542.5917160/1‑94 (MQ=60)
                                                                     GCTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTcatc                         >  SRR4302542.7163796/1‑90 (MQ=60)
                                                                      CTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTC                         >  SRR4302542.1419919/1‑94 (MQ=60)
                                                                      CTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCA                        >  SRR4302542.4486693/1‑94 (MQ=60)
                                                                      CTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCA                        >  SRR4302542.1086374/1‑94 (MQ=60)
                                                                      CTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCA                        >  SRR4302542.837497/1‑94 (MQ=60)
                                                                      CTCCTGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCA                        >  SRR4302542.2356137/1‑94 (MQ=60)
                                                                        CCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAA                       <  SRR4302542.4896150/94‑1 (MQ=60)
                                                                        CCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAA                       >  SRR4302542.4732091/1‑94 (MQ=60)
                                                                        CCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAA                       <  SRR4302542.4685152/94‑1 (MQ=60)
                                                                          TGTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAA                    >  SRR4302542.6910833/1‑94 (MQ=60)
                                                                           GTGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAG                   <  SRR4302542.4831887/94‑1 (MQ=60)
                                                                           GTGTAAACAC‑TGATAGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAG                   <  SRR4302542.5531984/94‑1 (MQ=60)
                                                                            TGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGGTTGGTAAAATAGCCGCCATTTTTCGTTTTTAACAAGCGCGGTCCCGTGCCGCTTACTCAAGAAA                   >  SRR4302542.2674658/1‑94 (MQ=60)
                                                                            TGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGA                  >  SRR4302542.2183793/1‑94 (MQ=60)
                                                                            TGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGA                  <  SRR4302542.741392/94‑1 (MQ=60)
                                                                            TGTAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGA                  <  SRR4302542.4678713/94‑1 (MQ=60)
                                                                              TAAACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAA                <  SRR4302542.1506540/94‑1 (MQ=60)
                                                                               AAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAA                <  SRR4302542.7143483/94‑1 (MQ=60)
                                                                                AACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAG               >  SRR4302542.3401029/1‑94 (MQ=60)
                                                                                AACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAG               >  SRR4302542.4058095/1‑94 (MQ=60)
                                                                                AACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGA              <  SRR4302542.6757317/94‑1 (MQ=60)
                                                                                 ACAC‑TGATTGCC‑‑CCCCCCCGTTGGTGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGGGCGATGCCGCCTACTTACGAAGAAAGAA             >  SRR4302542.1557520/1‑94 (MQ=60)
                                                                                 ACAC‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAA             <  SRR4302542.592467/94‑1 (MQ=60)
                                                                                    C‑TGATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATT           <  SRR4302542.1009020/94‑1 (MQ=60)
                                                                                    C‑TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTA          <  SRR4302542.1111105/94‑1 (MQ=60)
                                                                                    C‑TGATT‑CCC‑CCCCCCCGTTGATAGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTA          <  SRR4302542.6401509/94‑1 (MQ=60)
                                                                                      TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.3347367/94‑1 (MQ=60)
                                                                                      TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.6516525/94‑1 (MQ=60)
                                                                                      TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.1574552/94‑1 (MQ=60)
                                                                                      TGATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         >  SRR4302542.2823756/1‑94 (MQ=60)
                                                                                      TGATTGCC‑‑CCCCCCCGTTGATGAGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.54438/94‑1 (MQ=60)
                                                                                       TATTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.3593444/94‑1 (MQ=60)
                                                                                       GATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.1108903/94‑1 (MQ=60)
                                                                                       GATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.6405694/94‑1 (MQ=60)
                                                                                       GATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTAT         <  SRR4302542.6733634/94‑1 (MQ=60)
                                                                                       GATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATG        <  SRR4302542.7472714/94‑1 (MQ=60)
                                                                                       GATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATG        <  SRR4302542.1646540/94‑1 (MQ=60)
                                                                                       GATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATG        <  SRR4302542.7456914/94‑1 (MQ=60)
                                                                                        ATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATG        >  SRR4302542.1201163/1‑94 (MQ=60)
                                                                                        ATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGA       <  SRR4302542.2719917/94‑1 (MQ=60)
                                                                                        ATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGA       >  SRR4302542.4949636/1‑94 (MQ=60)
                                                                                        ATTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGA       <  SRR4302542.4059000/94‑1 (MQ=60)
                                                                                         TTGCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGA       <  SRR4302542.148363/94‑1 (MQ=60)
                                                                                         TTGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGAC      <  SRR4302542.5079407/94‑1 (MQ=60)
                                                                                          TGCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACG     >  SRR4302542.3385663/1‑94 (MQ=60)
                                                                                           GCC‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTTAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGT    >  SRR4302542.5297050/1‑94 (MQ=60)
                                                                                             CC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTT   >  SRR4302542.4494201/1‑94 (MQ=60)
                                                                                             CC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTT   >  SRR4302542.5687759/1‑94 (MQ=60)
                                                                                             CC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTT   >  SRR4302542.1839507/1‑94 (MQ=60)
                                                                                              C‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTG  <  SRR4302542.7480963/94‑1 (MQ=60)
                                                                                              C‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTG  <  SRR4302542.5164974/94‑1 (MQ=60)
                                                                                              C‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTG  >  SRR4302542.7212939/1‑94 (MQ=60)
                                                                                              |                                                                                              
ACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACAC‑TGATTGCCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTG  >  NC_000913/4606805‑4606991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑