Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,632,259 T→C A207A (GCA→GCG)  rob ← right origin‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,632,2590TC86.3% 682.9 / 99.0 285A207A (GCA→GCG) robright origin‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (18/21);  new base C (135/111);  total (153/132)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

Only 100 of 286 total aligned reads displayed.

CTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCAC  >  NC_000913/4632169‑4632351
                                                                                          |                                                                                            
cTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGc                                                                                            >  2:5417435/1‑93 (MQ=255)
cTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGc                                                                                            <  1:2792351/93‑1 (MQ=255)
   cACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTg                                                                                         >  1:4178349/1‑93 (MQ=255)
   cACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTg                                                                                         >  2:7462569/1‑93 (MQ=255)
   cACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTg                                                                                         <  2:2978322/93‑1 (MQ=255)
     cGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGAt                                                                                       <  2:7339433/93‑1 (MQ=255)
      gCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATc                                                                                      >  2:7007302/1‑93 (MQ=255)
      gCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATc                                                                                      >  1:4952191/1‑93 (MQ=255)
         ggTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTg                                                                                   <  1:5956963/93‑1 (MQ=255)
         ggTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTg                                                                                   >  2:1715744/1‑93 (MQ=255)
         ggTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTg                                                                                   >  1:337152/1‑93 (MQ=255)
         ggTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTg                                                                                   >  2:5758149/1‑93 (MQ=255)
            tCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGc                                                                                >  2:1605180/1‑93 (MQ=255)
              ccAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTa                                                                              >  1:5290342/1‑93 (MQ=255)
               cAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTaa                                                                             >  1:2966081/1‑93 (MQ=255)
               cAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTaa                                                                             >  2:211384/1‑93 (MQ=255)
               cAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTaa                                                                             >  2:1210825/1‑93 (MQ=255)
                 gACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAAcg                                                                           >  2:3816948/1‑93 (MQ=255)
                 gACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcg                                                                           >  2:4138313/1‑93 (MQ=255)
                 gACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcg                                                                           >  2:5656117/1‑93 (MQ=255)
                  aCCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcgc                                                                          <  1:1943050/93‑1 (MQ=255)
                     ttCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCggt                                                                       <  2:695608/93‑1 (MQ=255)
                     ttCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggt                                                                       <  1:7396547/93‑1 (MQ=255)
                     ttCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggt                                                                       <  1:7093860/93‑1 (MQ=255)
                      tCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtg                                                                      <  1:3106186/93‑1 (MQ=255)
                        aTAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCggtggt                                                                    >  1:4600502/1‑93 (MQ=255)
                        aTAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtggt                                                                    >  1:718161/1‑93 (MQ=255)
                         tAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGta                                                                   >  1:5759775/1‑93 (MQ=255)
                         tAGGTAAACATCACATATTCGCCACCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGta                                                                   >  2:243455/1‑93 (MQ=255)
                          aGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGtat                                                                  >  2:1110768/1‑93 (MQ=255)
                          aGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGtat                                                                  >  2:2275157/1‑93 (MQ=255)
                          aGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGtat                                                                  >  1:3727101/1‑93 (MQ=255)
                           ggTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGtata                                                                 <  1:6753675/93‑1 (MQ=255)
                            gTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAg                                                                >  1:6378954/1‑93 (MQ=255)
                             tAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGa                                                               <  1:244745/93‑1 (MQ=255)
                              aaaCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGaa                                                              >  1:5804214/1‑93 (MQ=255)
                              aaaCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGaa                                                              >  2:5869895/1‑93 (MQ=255)
                               aaCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAAt                                                             >  2:3605108/1‑93 (MQ=255)
                                aCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATa                                                            <  1:5480007/93‑1 (MQ=255)
                                aCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATa                                                            <  2:5102961/93‑1 (MQ=255)
                                 cATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAc                                                           >  1:291636/1‑93 (MQ=255)
                                 cATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAc                                                           >  1:2715911/1‑93 (MQ=255)
                                 cATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAc                                                           <  1:2486318/93‑1 (MQ=255)
                                  aTCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCT‑ATCCTGGGCTAACGCGGTGGTATAGAATACct                                                         <  2:1472098/93‑1 (MQ=255)
                                  aTCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAcc                                                          <  1:983962/93‑1 (MQ=255)
                                  aTCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAcc                                                          <  1:6905851/93‑1 (MQ=255)
                                  aTCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATACTGGGCTAACGCGGTGGTATAGAATAcc                                                          <  2:5893722/93‑1 (MQ=255)
                                   tCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACct                                                         >  2:4812740/1‑93 (MQ=255)
                                    cacaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctc                                                        <  1:4269062/93‑1 (MQ=255)
                                    cacaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctc                                                        <  2:4908041/93‑1 (MQ=255)
                                     acaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctct                                                       <  1:860735/93‑1 (MQ=255)
                                      caTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCtt                                                      >  2:6592868/1‑93 (MQ=255)
                                        tatTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGc                                                    >  1:7367846/1‑93 (MQ=255)
                                        tatTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGc                                                    <  2:3948319/93‑1 (MQ=255)
                                        tatTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGc                                                    >  1:1553709/1‑93 (MQ=255)
                                         atTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCt                                                   <  2:1968478/93‑1 (MQ=255)
                                          ttCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtc                                                  >  1:2579251/1‑93 (MQ=255)
                                             gccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtc                                               >  2:5362266/1‑93 (MQ=255)
                                             gccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtc                                               >  2:3986310/1‑93 (MQ=255)
                                             gccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtc                                               <  1:1098086/93‑1 (MQ=255)
                                              ccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtcg                                              <  2:7263227/93‑1 (MQ=255)
                                               cgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt                                             >  2:3356590/1‑93 (MQ=255)
                                                gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCgtcgtc                                            <  1:624622/93‑1 (MQ=255)
                                                gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCgtcgtc                                            >  2:2827278/1‑93 (MQ=255)
                                                 cccTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCt                                           <  1:157498/93‑1 (MQ=255)
                                                  ccTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCtt                                          >  2:790982/1‑93 (MQ=255)
                                                  ccTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCtt                                          >  2:6782841/1‑93 (MQ=255)
                                                  ccTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCtt                                          >  1:4589298/1‑93 (MQ=255)
                                                   cTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCttt                                         >  1:3596020/1‑93 (MQ=255)
                                                   cTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCttt                                         >  1:6996255/1‑93 (MQ=255)
                                                   cTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCttt                                         <  1:6098855/93‑1 (MQ=255)
                                                       agcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATcc                                     <  2:3207413/93‑1 (MQ=255)
                                                        gcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCt                                    >  1:4395613/1‑93 (MQ=255)
                                                           tCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGAc                                 <  1:1904618/93‑1 (MQ=255)
                                                           tCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGAc                                 >  2:6136008/1‑93 (MQ=255)
                                                             aCCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTc                               <  2:4323796/93‑1 (MQ=255)
                                                              ccGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCg                              >  1:4347216/1‑93 (MQ=255)
                                                                ggATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGa                            <  1:1767008/93‑1 (MQ=255)
                                                                ggATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGa                            <  2:5523802/93‑1 (MQ=255)
                                                                    gCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAcgcg                        >  1:6821634/1‑93 (MQ=255)
                                                                     ccccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGt                       >  2:1768451/1‑93 (MQ=255)
                                                                      cccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGtt                      <  1:2317631/93‑1 (MQ=255)
                                                                      cccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGtt                      >  1:5142105/1‑93 (MQ=255)
                                                                       ccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGttt                     >  2:466978/1‑93 (MQ=255)
                                                                       ccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGttt                     <  2:1801767/93‑1 (MQ=255)
                                                                        cGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttc                    <  1:1235751/93‑1 (MQ=255)
                                                                          tCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttcat                  <  2:269724/93‑1 (MQ=255)
                                                                            aGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttcattc                >  1:1102945/1‑93 (MQ=255)
                                                                            aGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttcattc                >  1:3612675/1‑93 (MQ=255)
                                                                             gTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttcattca               <  2:2226942/93‑1 (MQ=255)
                                                                                    gCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTa        <  1:5588780/93‑1 (MQ=255)
                                                                                    gCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTa        <  1:2514714/93‑1 (MQ=255)
                                                                                     ccATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTag       >  1:2637613/1‑93 (MQ=255)
                                                                                     ccATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTag       <  2:6339479/93‑1 (MQ=255)
                                                                                     ccATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTag       <  2:4251484/93‑1 (MQ=255)
                                                                                     ccATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTag       >  2:7153946/1‑93 (MQ=255)
                                                                                      cATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTaga      >  1:4132567/1‑93 (MQ=255)
                                                                                      cATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTaga      >  1:6582091/1‑93 (MQ=255)
                                                                                      cATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTaga      >  2:847340/1‑93 (MQ=255)
                                                                                       aTCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTagag     >  2:5248209/1‑93 (MQ=255)
                                                                                          |                                                                                            
CTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCAC  >  NC_000913/4632169‑4632351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACC  >  NC_000913/4632166‑4632352
                                                                                             |                                                                                             
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCT                                                                                               <  SRR4302542.6157362/94‑1 (MQ=60)
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCC                                                                                               >  SRR4302542.4055053/1‑94 (MQ=60)
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCC                                                                                               >  SRR4302542.285120/1‑94 (MQ=60)
 TCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCG                                                                                              <  SRR4302542.4279823/94‑1 (MQ=60)
 TCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCG                                                                                              >  SRR4302542.5581853/1‑94 (MQ=60)
  CCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGC                                                                                             >  SRR4302542.744287/1‑94 (MQ=60)
   CTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCC                                                                                            <  SRR4302542.2819223/94‑1 (MQ=60)
    TGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCT                                                                                           >  SRR4302542.3124128/1‑94 (MQ=60)
     GCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTG                                                                                          >  SRR4302542.4219552/1‑94 (MQ=60)
       ACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGAT                                                                                        >  SRR4302542.7253208/1‑94 (MQ=60)
        CGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATC                                                                                       >  SRR4302542.5000312/1‑94 (MQ=60)
          CCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCT                                                                                     <  SRR4302542.612793/94‑1 (MQ=60)
          CCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCT                                                                                     <  SRR4302542.2624972/94‑1 (MQ=60)
          CCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCCGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCT                                                                                     <  SRR4302542.568382/94‑1 (MQ=60)
           CGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTG                                                                                    >  SRR4302542.340080/1‑94 (MQ=60)
            GGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGG                                                                                   <  SRR4302542.5402285/94‑1 (MQ=60)
            GGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGG                                                                                   <  SRR4302542.1805298/94‑1 (MQ=60)
            GGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGG                                                                                   <  SRR4302542.6014647/94‑1 (MQ=60)
            GGTGCCCAGACCTTCATAGGTAAACATCACATGTTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGG                                                                                   <  SRR4302542.2647168/94‑1 (MQ=60)
              TTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGC                                                                                 >  SRR4302542.4968396/1‑94 (MQ=60)
               TCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCT                                                                                <  SRR4302542.1593196/94‑1 (MQ=60)
                CCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTA                                                                               <  SRR4302542.2858774/94‑1 (MQ=60)
                CCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTA                                                                               >  SRR4302542.5341762/1‑94 (MQ=60)
                 CCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAA                                                                              >  SRR4302542.2994497/1‑94 (MQ=60)
                 CCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAA                                                                              >  SRR4302542.5146390/1‑94 (MQ=60)
                  CAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCTGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAC                                                                             >  SRR4302542.2477573/1‑94 (MQ=60)
                   AGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACG                                                                            >  SRR4302542.251643/1‑94 (MQ=60)
                   AGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACG                                                                            >  SRR4302542.658214/1‑94 (MQ=60)
                     ACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCG                                                                          <  SRR4302542.1959853/94‑1 (MQ=60)
                     ACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCG                                                                          <  SRR4302542.2595408/94‑1 (MQ=60)
                      CCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGG                                                                         >  SRR4302542.3641657/1‑94 (MQ=60)
                       CTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGT                                                                        >  SRR4302542.3038781/1‑94 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCCCCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                       <  SRR4302542.3746940/94‑1 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                       <  SRR4302542.7308585/94‑1 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                       <  SRR4302542.2842775/94‑1 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                       <  SRR4302542.7468941/94‑1 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                       <  SRR4302542.7163455/94‑1 (MQ=60)
                         TCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGG                                                                      <  SRR4302542.3135712/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGT                                                                     >  SRR4302542.4645474/1‑94 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                     <  SRR4302542.4096523/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                     >  SRR4302542.724353/1‑94 (MQ=60)
                           ATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTA                                                                    >  SRR4302542.5815687/1‑94 (MQ=60)
                           ATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTA                                                                    >  SRR4302542.5815686/1‑94 (MQ=60)
                            TAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTAT                                                                   >  SRR4302542.3764009/1‑94 (MQ=60)
                            TAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTAT                                                                   <  SRR4302542.6924954/94‑1 (MQ=60)
                            TAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTAT                                                                   <  SRR4302542.707857/94‑1 (MQ=60)
                             AGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATA                                                                  <  SRR4302542.4375429/94‑1 (MQ=60)
                             AGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATA                                                                  >  SRR4302542.2361894/1‑94 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                 >  SRR4302542.442262/1‑94 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                 >  SRR4302542.6441760/1‑94 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                 >  SRR4302542.6700963/1‑94 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                 <  SRR4302542.6820114/94‑1 (MQ=60)
                               GTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGA                                                                <  SRR4302542.4193158/94‑1 (MQ=60)
                                TAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAA                                                               <  SRR4302542.246846/94‑1 (MQ=60)
                                TAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAA                                                               >  SRR4302542.5860543/1‑94 (MQ=60)
                                 AAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAAT                                                              <  SRR4302542.2690767/94‑1 (MQ=60)
                                  AACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATA                                                             >  SRR4302542.5348032/1‑94 (MQ=60)
                                   ACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAC                                                            <  SRR4302542.5533234/94‑1 (MQ=60)
                                   ACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAC                                                            >  SRR4302542.294182/1‑94 (MQ=60)
                                   ACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAC                                                            >  SRR4302542.2742102/1‑94 (MQ=60)
                                    CATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACC                                                           >  SRR4302542.333513/1‑94 (MQ=60)
                                    CATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACC                                                           <  SRR4302542.2510437/94‑1 (MQ=60)
                                     ATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCT                                                          <  SRR4302542.6973648/94‑1 (MQ=60)
                                     ATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCT                                                          <  SRR4302542.992439/94‑1 (MQ=60)
                                      cccCATATCCGCCGCCCTGCACCATCACCGGAGGCCCCGTCAGTACATAGCCATCCGCCGGATCTGGGGCTAACGCGGTGGAATAGAATCCCTC                                                         <  SRR4302542.6264900/91‑1 (MQ=47)
                                      TCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTC                                                         >  SRR4302542.7174680/1‑94 (MQ=60)
                                      TCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTC                                                         <  SRR4302542.1827542/94‑1 (MQ=60)
                                       CACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCT                                                        <  SRR4302542.4311073/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                       <  SRR4302542.6717885/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                       <  SRR4302542.5660927/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                       <  SRR4302542.868147/94‑1 (MQ=60)
                                         CATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTTGGCTAACGCGGTGGTATAGAATACCTCTTG                                                      <  SRR4302542.3604525/94‑1 (MQ=60)
                                          ATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGC                                                     >  SRR4302542.1567118/1‑94 (MQ=60)
                                          ATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGC                                                     >  SRR4302542.7440004/1‑94 (MQ=60)
                                          ATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGC                                                     >  SRR4302542.5231813/1‑94 (MQ=60)
                                           TATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCT                                                    <  SRR4302542.6911707/94‑1 (MQ=60)
                                           TATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCT                                                    <  SRR4302542.2668767/94‑1 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                   >  SRR4302542.2512367/1‑94 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                   <  SRR4302542.1187505/94‑1 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                   >  SRR4302542.2604253/1‑94 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                   <  SRR4302542.2425926/94‑1 (MQ=60)
                                             TTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCG                                                  >  SRR4302542.3638288/1‑94 (MQ=60)
                                               CGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTC                                                >  SRR4302542.6176525/1‑94 (MQ=60)
                                               CGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTC                                                >  SRR4302542.6127795/1‑94 (MQ=60)
                                                GCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCG                                               <  SRR4302542.7006158/94‑1 (MQ=60)
                                                GCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCG                                               <  SRR4302542.1107495/94‑1 (MQ=60)
                                                GCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCG                                               <  SRR4302542.1107494/94‑1 (MQ=60)
                                                  CGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGcataaatgaggaaaatggggaactggaag                                             <  SRR4302542.6414355/94‑30 (MQ=60)
                                                   GCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCT                                            <  SRR4302542.629999/94‑1 (MQ=60)
                                                   GCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCT                                            >  SRR4302542.4469021/1‑94 (MQ=60)
                                                    CCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTT                                           <  SRR4302542.158842/94‑1 (MQ=60)
                                                    CCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTT                                           >  SRR4302542.1440086/1‑94 (MQ=60)
                                                    CCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTT                                           >  SRR4302542.4634182/1‑94 (MQ=60)
                                                     CCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTT                                          >  SRR4302542.4505050/1‑94 (MQ=60)
                                                     CCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTT                                          >  SRR4302542.7064991/1‑94 (MQ=60)
                                                     CCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTT                                          >  SRR4302542.3629555/1‑94 (MQ=60)
                                                      CTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATGGAATACCTCTTGCTCGTCGTCTTTA                                         >  SRR4302542.4406457/1‑94 (MQ=60)
                                                      CTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTA                                         <  SRR4302542.6157880/94‑1 (MQ=60)
                                                      CTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTA                                         >  SRR4302542.7565440/1‑94 (MQ=60)
                                                      CTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTA                                         >  SRR4302542.1658902/1‑94 (MQ=60)
                                                      CTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTA                                         <  SRR4302542.7117485/94‑1 (MQ=60)
                                                       TGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTAT                                        <  SRR4302542.7440108/94‑1 (MQ=60)
                                                       TGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTAT                                        <  SRR4302542.6866993/94‑1 (MQ=60)
                                                        GCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATC                                       <  SRR4302542.2048693/94‑1 (MQ=60)
                                                         CAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCC                                      <  SRR4302542.1118638/94‑1 (MQ=60)
                                                          AGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCT                                     >  SRR4302542.4438733/1‑94 (MQ=60)
                                                           GCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTG                                    >  SRR4302542.5618327/1‑94 (MQ=60)
                                                           GCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTG                                    >  SRR4302542.4909918/1‑94 (MQ=60)
                                                            CATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGA                                   >  SRR4302542.3258955/1‑94 (MQ=60)
                                                             ATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGAC                                  >  SRR4302542.4250198/1‑94 (MQ=60)
                                                             ATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGAC                                  >  SRR4302542.431286/1‑94 (MQ=60)
                                                              TCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACT                                 <  SRR4302542.1921079/94‑1 (MQ=60)
                                                              TCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACT                                 <  SRR4302542.4290145/94‑1 (MQ=60)
                                                               CACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTC                                >  SRR4302542.1576266/1‑94 (MQ=60)
                                                                ACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCG                               >  SRR4302542.7497843/1‑94 (MQ=60)
                                                                ACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCG                               >  SRR4302542.4158386/1‑94 (MQ=60)
                                                                ACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCG                               >  SRR4302542.4389917/1‑94 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCGTCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGCCTCGGAC                            <  SRR4302542.6989902/94‑1 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            <  SRR4302542.2956139/94‑1 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            <  SRR4302542.1782328/94‑1 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            <  SRR4302542.2544476/94‑1 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            >  SRR4302542.3485916/1‑94 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            >  SRR4302542.909898/1‑94 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            <  SRR4302542.4173829/94‑1 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                            <  SRR4302542.1928701/94‑1 (MQ=60)
                                                                      TGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCG                         >  SRR4302542.6888689/1‑94 (MQ=60)
                                                                       GCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGT                        <  SRR4302542.6365663/94‑1 (MQ=60)
                                                                        CCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTT                       >  SRR4302542.5880100/1‑94 (MQ=60)
                                                                        CCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTT                       >  SRR4302542.5192089/1‑94 (MQ=60)
                                                                        CCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTT                       >  SRR4302542.597875/1‑94 (MQ=60)
                                                                         CCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTT                      <  SRR4302542.2337512/94‑1 (MQ=60)
                                                                           CGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCA                    >  SRR4302542.1955184/1‑94 (MQ=60)
                                                                           CGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCA                    <  SRR4302542.1246378/94‑1 (MQ=60)
                                                                           CGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCA                    >  SRR4302542.1493917/1‑94 (MQ=60)
                                                                            GTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCAT                   <  SRR4302542.6507115/94‑1 (MQ=60)
                                                                             TCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATT                  >  SRR4302542.5609768/1‑94 (MQ=60)
                                                                              CAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTC                 >  SRR4302542.1112378/1‑94 (MQ=60)
                                                                              CAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTC                 >  SRR4302542.3646341/1‑94 (MQ=60)
                                                                               AGTACATAG‑CATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAG               >  SRR4302542.6385730/1‑94 (MQ=60)
                                                                                GTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAG               <  SRR4302542.2482362/94‑1 (MQ=60)
                                                                                GTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAG               >  SRR4302542.5032768/1‑94 (MQ=60)
                                                                                    ATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCG           <  SRR4302542.5256906/94‑1 (MQ=60)
                                                                                     TAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGT          >  SRR4302542.4635818/1‑94 (MQ=60)
                                                                                       GCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAG        >  SRR4302542.1579685/1‑94 (MQ=60)
                                                                                       GCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAG        >  SRR4302542.2663087/1‑94 (MQ=60)
                                                                                       GCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAG        <  SRR4302542.2539109/94‑1 (MQ=60)
                                                                                       GCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAG        <  SRR4302542.2949561/94‑1 (MQ=60)
                                                                                       GCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAG        <  SRR4302542.5643088/94‑1 (MQ=60)
                                                                                        CCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGA       >  SRR4302542.4173284/1‑94 (MQ=60)
                                                                                        CCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGA       >  SRR4302542.6646942/1‑94 (MQ=60)
                                                                                         CATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAG      <  SRR4302542.2797630/94‑1 (MQ=60)
                                                                                          ATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGC     >  SRR4302542.716963/1‑94 (MQ=60)
                                                                                            CCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCAC   >  SRR4302542.662836/1‑94 (MQ=60)
                                                                                             CGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACC  <  SRR4302542.5744602/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACC  >  NC_000913/4632166‑4632352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑