Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,636,923 T→C W393R (TGG→CGG)  creD → CreD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,9230TC84.0% 597.1 / 107.7 270W393R (TGG→CGG) creDCreD
Reads supporting (aligned to +/- strand):  ref base T (24/19);  new base C (122/104);  total (147/123)
Fisher's exact test for biased strand distribution p-value = 8.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-02

Only 100 of 271 total aligned reads displayed.

ATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAA  >  NC_000913/4636833‑4637014
                                                                                           |                                                                                           
aTGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTgg                                                                                           <  2:3367535/93‑1 (MQ=255)
 tGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCggg                                                                                          >  1:4008957/1‑93 (MQ=255)
 tGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCggg                                                                                          <  1:5550347/93‑1 (MQ=255)
 tGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCggg                                                                                          >  1:5399728/1‑93 (MQ=255)
  gAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCgggg                                                                                         <  1:3335081/93‑1 (MQ=255)
    aCGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGAc                                                                                       <  1:6434660/93‑1 (MQ=255)
    aCGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGAc                                                                                       <  1:4564223/93‑1 (MQ=255)
    aCGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGAc                                                                                       <  2:3659804/93‑1 (MQ=255)
      ggTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGCCTg                                                                                     >  1:6969117/1‑93 (MQ=255)
      ggTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTg                                                                                     >  2:6195527/1‑93 (MQ=255)
      ggTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTg                                                                                     >  1:3358471/1‑93 (MQ=255)
       gTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGc                                                                                    >  1:4929795/1‑93 (MQ=255)
       gTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGc                                                                                    >  1:149887/1‑93 (MQ=255)
        tatttattTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCt                                                                                   >  2:4538686/1‑93 (MQ=255)
        tatttattTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCt                                                                                   >  1:1380046/1‑93 (MQ=255)
         atttatttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTc                                                                                  <  2:4222777/93‑1 (MQ=255)
          tttatttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCa                                                                                 <  1:5316765/93‑1 (MQ=255)
            tatttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAAc                                                                               <  1:2041614/93‑1 (MQ=255)
             atttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAAct                                                                              >  2:3024306/1‑93 (MQ=255)
             atttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAAct                                                                              >  2:3736400/1‑93 (MQ=255)
              tttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAActc                                                                             <  2:184246/93‑1 (MQ=255)
               ttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAActct                                                                            <  1:2585143/93‑1 (MQ=255)
                    aGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGa                                                                       >  2:111483/1‑93 (MQ=255)
                    aGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGa                                                                       >  1:4382463/1‑93 (MQ=255)
                    aGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGa                                                                       >  2:3932239/1‑93 (MQ=255)
                    aGCGGTATTGAAAGGTTGGTGCAAAAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGa                                                                       >  2:7365264/1‑93 (MQ=255)
                      cGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATa                                                                     >  1:6554359/1‑93 (MQ=255)
                       ggTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAg                                                                    >  2:7145686/1‑93 (MQ=255)
                         tATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAgcg                                                                  >  2:3489392/1‑93 (MQ=255)
                           ttGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAgcgcg                                                                >  2:3863569/1‑93 (MQ=255)
                             gAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCt                                                              <  2:4139136/93‑1 (MQ=255)
                               aaGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCACCTCTGCCGATAGCGCGCtgt                                                            <  1:1431548/93‑1 (MQ=255)
                                 ggTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCtgttg                                                          <  2:568828/93‑1 (MQ=255)
                                 ggTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCtgttg                                                          >  1:4724522/1‑93 (MQ=255)
                                  gTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCtgttgt                                                         <  2:171511/93‑1 (MQ=255)
                                  gTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCtgttgt                                                         <  1:7272260/93‑1 (MQ=255)
                                  gTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCtgttgt                                                         >  2:2612032/1‑93 (MQ=255)
                                   ttGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGCGACTGCTCAACTCTGCCGATAGCGCGCTgttgtt                                                        >  1:2837657/1‑93 (MQ=255)
                                   ttGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTgttgtt                                                        >  2:1996987/1‑93 (MQ=255)
                                    tGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGttgttg                                                       <  2:1332370/93‑1 (MQ=255)
                                    tGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGttgttg                                                       <  2:2975648/93‑1 (MQ=255)
                                         cAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAc                                                  >  1:5091107/1‑93 (MQ=255)
                                         cAACAGCATGTTGTTTACCCTCGC‑GCTGGTGTTGCTGGATGGTGCGAGGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAc                                                  >  2:3321642/1‑93 (MQ=255)
                                          aaCAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAcc                                                 >  1:51063/1‑93 (MQ=255)
                                          aaCAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAcc                                                 >  1:893318/1‑93 (MQ=255)
                                          aaCAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAcc                                                 >  2:181023/1‑93 (MQ=255)
                                           aCAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCa                                                >  2:2094121/1‑93 (MQ=255)
                                            cAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAg                                               <  1:2221629/93‑1 (MQ=255)
                                             aGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgt                                              <  1:219606/93‑1 (MQ=255)
                                              gCATGTTGTTTCCCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             <  1:3930627/93‑1 (MQ=255)
                                              gCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             >  1:1021606/1‑93 (MQ=255)
                                              gCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             <  1:2776287/93‑1 (MQ=255)
                                              gCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             >  1:35847/1‑93 (MQ=255)
                                              gCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             <  2:5639079/93‑1 (MQ=255)
                                              gCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             >  2:4633863/1‑93 (MQ=255)
                                              gCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtg                                             >  1:7521550/1‑93 (MQ=255)
                                               cATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtgt                                            <  1:18702/93‑1 (MQ=255)
                                                 tgttgtTTACCCTCGCGGCTGTTGTTGCTGGATGGGGTGATGCGGGGCCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGtgtg                                           >  1:3052507/1‑93 (MQ=255)
                                                  gttgttTACCCTCGC‑GCTGTTGTTGCTGGATGGGGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCt                                         >  1:6082655/1‑93 (MQ=255)
                                                   ttgttTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtg                                        >  2:7082333/1‑93 (MQ=255)
                                                   ttgttTACCCTCGC‑GCTGTTGTTGCGGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtg                                        <  1:5804630/93‑1 (MQ=255)
                                                    tgttTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtgg                                       <  2:1677638/93‑1 (MQ=255)
                                                    tgttTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtgg                                       <  1:1054712/93‑1 (MQ=255)
                                                     gttTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggt                                      <  2:234964/93‑1 (MQ=255)
                                                     gttTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggt                                      >  1:1147635/1‑93 (MQ=255)
                                                     gttTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggt                                      >  1:4261114/1‑93 (MQ=255)
                                                      tttACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtg                                     <  1:3738337/93‑1 (MQ=255)
                                                      tttACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtg                                     <  2:3122179/93‑1 (MQ=255)
                                                      tttACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtg                                     >  1:1624752/1‑93 (MQ=255)
                                                      tttACCCTCGC‑GCTGTTGTTGCTGGATAGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtg                                     >  2:6431424/1‑93 (MQ=255)
                                                       ttACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGTGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtgg                                    >  2:3647220/1‑93 (MQ=255)
                                                        tACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtggt                                   <  2:6274497/93‑1 (MQ=255)
                                                         aNCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtggtg                                  <  2:5595271/93‑1 (MQ=255)
                                                          cccTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtggtgg                                 >  2:1931527/1‑93 (MQ=255)
                                                           ccTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGgc                                <  2:6018178/93‑1 (MQ=255)
                                                           ccTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGgc                                >  2:4884925/1‑93 (MQ=255)
                                                           ccTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGgc                                >  2:4807504/1‑93 (MQ=255)
                                                            cTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGgcg                               >  2:652712/1‑93 (MQ=255)
                                                            cTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGgcg                               >  1:6596758/1‑93 (MQ=255)
                                                             tCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGgcgc                              >  1:4461737/1‑93 (MQ=255)
                                                               gc‑gcTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTg                            <  2:692180/93‑1 (MQ=255)
                                                               gc‑gcTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTg                            <  2:4982155/93‑1 (MQ=255)
                                                                  gcTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGc                          >  2:4383202/1‑93 (MQ=255)
                                                                   cTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGcc                         <  2:2235314/93‑1 (MQ=255)
                                                                   cTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGcc                         <  1:3480946/93‑1 (MQ=255)
                                                                    tgttgtTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCg                        >  2:2316841/1‑93 (MQ=255)
                                                                       tgttgCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCa                     <  2:5409985/93‑1 (MQ=255)
                                                                        gttgCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCat                    <  1:1326623/93‑1 (MQ=255)
                                                                        gttgCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCat                    <  1:5607687/93‑1 (MQ=255)
                                                                          tgCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCatga                  <  2:2753460/93‑1 (MQ=255)
                                                                          tgCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCatga                  <  2:5298266/93‑1 (MQ=255)
                                                                           gCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCatgat                 <  2:5142736/93‑1 (MQ=255)
                                                                           gCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCatgat                 <  1:6920347/93‑1 (MQ=255)
                                                                             tGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGt               <  1:4149716/93‑1 (MQ=255)
                                                                              ggATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGtt              >  2:5906162/1‑93 (MQ=255)
                                                                              ggATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGtt              <  2:4011250/93‑1 (MQ=255)
                                                                               gATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGttt             <  1:3838055/93‑1 (MQ=255)
                                                                                 tGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTtgt           <  2:4063396/93‑1 (MQ=255)
                                                                                  ggTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTtgtg          >  2:6783303/1‑93 (MQ=255)
                                                                                        aTGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGt    <  1:6192059/93‑1 (MQ=255)
                                                                                           |                                                                                           
ATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAA  >  NC_000913/4636833‑4637014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATA  >  NC_000913/4636830‑4637016
                                                                                              |                                                                                             
ATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGC                                                                                               >  SRR4302542.136701/1‑94 (MQ=60)
 TAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCG                                                                                              >  SRR4302542.3024429/1‑94 (MQ=60)
 TAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCG                                                                                              >  SRR4302542.2381767/1‑94 (MQ=60)
 TAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCG                                                                                              >  SRR4302542.975162/1‑94 (MQ=60)
   ATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGG                                                                                            >  SRR4302542.5452177/1‑94 (MQ=60)
   ATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGG                                                                                            <  SRR4302542.565150/94‑1 (MQ=60)
   ATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGG                                                                                            >  SRR4302542.4048485/1‑94 (MQ=60)
    TGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGA                                                                                           <  SRR4302542.5407928/94‑1 (MQ=60)
    TGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGG                                                                                           <  SRR4302542.5604281/94‑1 (MQ=60)
    TGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGG                                                                                           <  SRR4302542.3602857/94‑1 (MQ=60)
    TGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCCGGATGGTGTGATGTGGGG                                                                                           <  SRR4302542.960771/94‑1 (MQ=60)
     GAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGA                                                                                          <  SRR4302542.3366463/94‑1 (MQ=60)
     GAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGA                                                                                          <  SRR4302542.3777137/94‑1 (MQ=60)
      AACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGGGGGATGGGGGGAC                                                                                         >  SRR4302542.5028614/1‑94 (MQ=60)
       ACGGTATTTATTTGCGAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACT                                                                                        <  SRR4302542.1273726/94‑1 (MQ=60)
       ACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACT                                                                                        <  SRR4302542.5673394/94‑1 (MQ=60)
       ACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACT                                                                                        <  SRR4302542.6498054/94‑1 (MQ=60)
       ACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACT                                                                                        <  SRR4302542.4608905/94‑1 (MQ=60)
        CGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGCCTG                                                                                       >  SRR4302542.7037579/1‑94 (MQ=60)
        CGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTG                                                                                       <  SRR4302542.393179/94‑1 (MQ=60)
        CGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTG                                                                                       >  SRR4302542.3390042/1‑94 (MQ=60)
         GGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGC                                                                                      >  SRR4302542.4977715/1‑94 (MQ=60)
         GGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGC                                                                                      <  SRR4302542.2084886/94‑1 (MQ=60)
         GGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGC                                                                                      >  SRR4302542.151153/1‑94 (MQ=60)
          GTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCT                                                                                     >  SRR4302542.1468042/1‑94 (MQ=60)
          GTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCT                                                                                     >  SRR4302542.2033613/1‑94 (MQ=60)
          GTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCT                                                                                     >  SRR4302542.1391974/1‑94 (MQ=60)
            ATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCA                                                                                   <  SRR4302542.847069/94‑1 (MQ=60)
            ATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCA                                                                                   >  SRR4302542.82467/1‑94 (MQ=60)
            ATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCA                                                                                   <  SRR4302542.3382932/94‑1 (MQ=60)
             TTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAA                                                                                  <  SRR4302542.5368417/94‑1 (MQ=60)
               TATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACT                                                                                <  SRR4302542.2059269/94‑1 (MQ=60)
                ATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTC                                                                               >  SRR4302542.4727362/1‑94 (MQ=60)
                 TTTGCAAGCGGTATTGAAAGGTTGGGGCAACAGCATGTTTTTTACCCTCGC‑GCTGTTGTTTCTGGATGGGGTGATGCGGGGACTGCTCAACTCT                                                                              >  SRR4302542.7533307/1‑94 (MQ=60)
                  TTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTG                                                                             <  SRR4302542.2610187/94‑1 (MQ=60)
                    GCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCC                                                                           <  SRR4302542.5149655/94‑1 (MQ=60)
                      AAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGA                                                                         >  SRR4302542.4425466/1‑94 (MQ=60)
                      AAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGA                                                                         >  SRR4302542.1443464/1‑94 (MQ=60)
                       AGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGAT                                                                        <  SRR4302542.5669231/94‑1 (MQ=60)
                       AGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGAT                                                                        >  SRR4302542.4313480/1‑94 (MQ=60)
                       AGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGAT                                                                        >  SRR4302542.3023943/1‑94 (MQ=60)
                        GCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATA                                                                       >  SRR4302542.6618970/1‑94 (MQ=60)
                         CGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAG                                                                      >  SRR4302542.99392/1‑94 (MQ=60)
                          GGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGC                                                                     <  SRR4302542.5199897/94‑1 (MQ=60)
                           GTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCG                                                                    <  SRR4302542.2725512/94‑1 (MQ=60)
                           GTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCG                                                                    >  SRR4302542.1770567/1‑94 (MQ=60)
                             ATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCG                                                                  >  SRR4302542.3773132/1‑94 (MQ=60)
                               TGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCT                                                                >  SRR4302542.1638672/1‑94 (MQ=60)
                               TGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCT                                                                <  SRR4302542.71743/94‑1 (MQ=60)
                               TGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCT                                                                >  SRR4302542.3924464/1‑94 (MQ=60)
                                  AAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTT                                                             <  SRR4302542.413794/94‑1 (MQ=60)
                                  AAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCACCTCTGCCGATAGCGCGCTGTT                                                             <  SRR4302542.1443923/94‑1 (MQ=60)
                                  AAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTT                                                             <  SRR4302542.3632551/94‑1 (MQ=60)
                                  AAGGTTGGTGCAACAGCATGTTG‑TTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTG                                                            >  SRR4302542.3564160/1‑94 (MQ=60)
                                   AGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTG                                                            <  SRR4302542.6253919/94‑1 (MQ=60)
                                   AGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTG                                                            >  SRR4302542.4770570/1‑94 (MQ=60)
                                   AGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTG                                                            <  SRR4302542.6827473/94‑1 (MQ=60)
                                     GTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGCGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTT                                                          >  SRR4302542.2864929/1‑94 (MQ=60)
                                     GTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTT                                                          <  SRR4302542.7343544/94‑1 (MQ=60)
                                           GCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAC                                                    >  SRR4302542.5140589/1‑94 (MQ=60)
                                            CAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACC                                                   >  SRR4302542.3997794/1‑94 (MQ=60)
                                            CAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACC                                                   >  SRR4302542.1895370/1‑94 (MQ=60)
                                            CAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACC                                                   >  SRR4302542.51475/1‑94 (MQ=60)
                                            CAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACC                                                   >  SRR4302542.901052/1‑94 (MQ=60)
                                             AACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCA                                                  <  SRR4302542.227282/94‑1 (MQ=60)
                                               CAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGT                                                <  SRR4302542.2240740/94‑1 (MQ=60)
                                                AGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTG                                               <  SRR4302542.221503/94‑1 (MQ=60)
                                                AGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTG                                               >  SRR4302542.7595515/1‑94 (MQ=60)
                                                AGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTG                                               >  SRR4302542.1030410/1‑94 (MQ=60)
                                                AGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTG                                               >  SRR4302542.36168/1‑94 (MQ=60)
                                                 GCATGTTGTTTCCCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGT                                              <  SRR4302542.3969413/94‑1 (MQ=60)
                                                 GCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGT                                              >  SRR4302542.257925/1‑94 (MQ=60)
                                                 GCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGT                                              >  SRR4302542.745694/1‑94 (MQ=60)
                                                 GCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGT                                              <  SRR4302542.2803024/94‑1 (MQ=60)
                                                  CATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGATCCAGTGTG                                             <  SRR4302542.4381963/94‑1 (MQ=60)
                                                  CATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTG                                             >  SRR4302542.1035548/1‑94 (MQ=60)
                                                  CATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTG                                             >  SRR4302542.5522232/1‑94 (MQ=60)
                                                  CATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTG                                             <  SRR4302542.1093466/94‑1 (MQ=60)
                                                  CATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTG                                             <  SRR4302542.3419315/94‑1 (MQ=60)
                                                  CATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTG                                             <  SRR4302542.18866/94‑1 (MQ=60)
                                                   ATGTTGTTTACCCTCGCGGCTGTTGTTGCTGGATGGGGTGATGCGGGGCCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTG                                             >  SRR4302542.3081636/1‑94 (MQ=60)
                                                    TGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCT                                           >  SRR4302542.4969237/1‑94 (MQ=60)
                                                    TGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGGGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCT                                           >  SRR4302542.6141551/1‑94 (MQ=60)
                                                      TTGTTTACCCTCGC‑GCTGTTGTTGCGGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGG                                         <  SRR4302542.5860963/94‑1 (MQ=60)
                                                       TGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGT                                        <  SRR4302542.1063777/94‑1 (MQ=60)
                                                       TGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGT                                        <  SRR4302542.6835726/94‑1 (MQ=60)
                                                       TGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGT                                        >  SRR4302542.1157465/1‑94 (MQ=60)
                                                       TGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGT                                        >  SRR4302542.4303069/1‑94 (MQ=60)
                                                        TTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTG                                       >  SRR4302542.4695633/1‑94 (MQ=60)
                                                        GTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTG                                       >  SRR4302542.1638774/1‑94 (MQ=60)
                                                         TTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGG                                      <  SRR4302542.7029069/94‑1 (MQ=60)
                                                         TTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGG                                      <  SRR4302542.6209547/94‑1 (MQ=60)
                                                         TTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGG                                      <  SRR4302542.3775338/94‑1 (MQ=60)
                                                          TTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGT                                     >  SRR4302542.1481466/1‑94 (MQ=60)
                                                          TTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGT                                     >  SRR4302542.4100382/1‑94 (MQ=60)
                                                          TTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGT                                     >  SRR4302542.3377767/1‑94 (MQ=60)
                                                          TTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGT                                     >  SRR4302542.5652850/1‑94 (MQ=60)
                                                           TACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTG                                    >  SRR4302542.1089506/1‑94 (MQ=60)
                                                            ACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGG                                   >  SRR4302542.6430786/1‑94 (MQ=60)
                                                            ACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGG                                   <  SRR4302542.4028399/94‑1 (MQ=60)
                                                            ACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGG                                   <  SRR4302542.1247749/94‑1 (MQ=60)
                                                             CCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGC                                  >  SRR4302542.58828/1‑94 (MQ=60)
                                                              CCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCG                                 >  SRR4302542.6661754/1‑94 (MQ=60)
                                                               CTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGC                                >  SRR4302542.4505501/1‑94 (MQ=60)
                                                                     GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCC                           >  SRR4302542.1845776/1‑94 (MQ=60)
                                                                     GCTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCC                           >  SRR4302542.6659697/1‑94 (MQ=60)
                                                                      CTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCG                          <  SRR4302542.5428991/94‑1 (MQ=60)
                                                                      CTGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCG                          <  SRR4302542.3513523/94‑1 (MQ=60)
                                                                       TGTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGG                         >  SRR4302542.1390831/1‑94 (MQ=60)
                                                                        GTTGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGC                        >  SRR4302542.4527768/1‑94 (MQ=60)
                                                                         tggtggcgGGATGGTGTGAGGCGGGGACTGCTCACCTCTGCCGATAGCGCGCTGTTGTTGGGACCCAGTGTGCTGGGGGTGGCCCTGGCCGGCA                       <  SRR4302542.7478087/86‑1 (MQ=60)
                                                                          TGTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCAT                      >  SRR4302542.3084266/1‑94 (MQ=60)
                                                                           GTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATG                     <  SRR4302542.5662166/94‑1 (MQ=60)
                                                                           GTTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATG                     <  SRR4302542.1338049/94‑1 (MQ=60)
                                                                            TTGCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGA                    >  SRR4302542.4943202/1‑94 (MQ=60)
                                                                              GCTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATG                  <  SRR4302542.6988291/94‑1 (MQ=60)
                                                                               CTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGT                 >  SRR4302542.6245465/1‑94 (MQ=60)
                                                                               CTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGT                 >  SRR4302542.1419930/1‑94 (MQ=60)
                                                                               CTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGT                 >  SRR4302542.2931383/1‑94 (MQ=60)
                                                                               CTGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGT                 <  SRR4302542.6519189/94‑1 (MQ=60)
                                                                                TGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTT                <  SRR4302542.4190588/94‑1 (MQ=60)
                                                                                TGGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTT                <  SRR4302542.2845469/94‑1 (MQ=60)
                                                                                 GGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGTATGATGTTT               <  SRR4302542.5296126/94‑1 (MQ=60)
                                                                                 GGATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTT               <  SRR4302542.4296766/94‑1 (MQ=60)
                                                                                  GATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTG              <  SRR4302542.3876005/94‑1 (MQ=60)
                                                                                  GATGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTG              <  SRR4302542.447287/94‑1 (MQ=60)
                                                                                    TGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTG            <  SRR4302542.5004834/94‑1 (MQ=60)
                                                                                    TGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCGGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTG            <  SRR4302542.272327/94‑1 (MQ=60)
                                                                                    TGGTGTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTG            >  SRR4302542.3265325/1‑94 (MQ=60)
                                                                                        GTGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCC        <  SRR4302542.4467612/94‑1 (MQ=60)
                                                                                         TGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCG       <  SRR4302542.7012299/94‑1 (MQ=60)
                                                                                         TGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCG       >  SRR4302542.658689/1‑94 (MQ=60)
                                                                                         TGATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCG       >  SRR4302542.222099/1‑94 (MQ=60)
                                                                                           ATGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTA     <  SRR4302542.6252312/94‑1 (MQ=60)
                                                                                            TGCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAA    >  SRR4302542.2115686/1‑94 (MQ=60)
                                                                                             GTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAAT   <  SRR4302542.2142924/94‑1 (MQ=60)
                                                                                             GCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAAT   <  SRR4302542.3712228/94‑1 (MQ=60)
                                                                                             GCGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAAT   <  SRR4302542.5157689/94‑1 (MQ=60)
                                                                                              CGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATA  <  SRR4302542.1443041/94‑1 (MQ=60)
                                                                                              |                                                                                             
ATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGC‑GCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATA  >  NC_000913/4636830‑4637016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑