Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,879 Δ1 bp coding (1005/1434 nt) ygjI → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,8780G.99.8% 1913.8 / ‑3.5 400coding (1004/1434 nt)ygjIputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (179/220);  total (179/221)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 448 total aligned reads displayed.

GACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAT  >  NC_000913/3224783‑3224971
                                                                                               |                                                                                              
     cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAg                                                                                          <  1:3100993/96‑1 (MQ=255)
       aCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCg                                                                                        >  1:13063928/1‑96 (MQ=255)
         gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTg                                                                                      >  1:2625597/1‑96 (MQ=255)
         gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTg                                                                                      >  1:453630/1‑96 (MQ=255)
         gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTg                                                                                      <  2:6178956/96‑1 (MQ=255)
          ctggTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTgg                                                                                     <  1:11999508/94‑1 (MQ=255)
          ccGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTgg                                                                                     >  2:29490/1‑96 (MQ=255)
           cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGa                                                                                    <  1:10187846/96‑1 (MQ=255)
           cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGa                                                                                    <  2:7654456/96‑1 (MQ=255)
            ggTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGAt                                                                                   <  2:13580320/96‑1 (MQ=255)
            ggTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGAt                                                                                   <  2:12509163/96‑1 (MQ=255)
             gTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGAtt                                                                                  <  1:8830610/96‑2 (MQ=255)
             gTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATc                                                                                  <  2:8647880/96‑1 (MQ=255)
               gtaaaTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCTGGCGC‑GCGCAGCGTGGATCCa                                                                                <  1:12860400/94‑1 (MQ=255)
               gAAAATTTTCTTCTCCGAACTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCa                                                                                <  1:10001627/96‑1 (MQ=255)
               gAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATcgc                                                                                <  1:11776345/96‑3 (MQ=255)
                  aaTTTTCTTCTCCGACATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGtt                                                                             <  1:13728611/96‑1 (MQ=255)
                  aaTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGtt                                                                             >  1:9641192/1‑96 (MQ=255)
                   aTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTc                                                                            <  2:3537688/96‑1 (MQ=255)
                    ttttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGtttc                                                                           <  1:3412799/96‑3 (MQ=255)
                     tttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGACCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGATCCt                                                                          <  2:5716417/96‑1 (MQ=255)
                     tttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCt                                                                          >  1:14347569/1‑96 (MQ=255)
                      ttcttcTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTg                                                                         >  2:249465/1‑96 (MQ=255)
                         ttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATc                                                                      <  2:10178890/96‑1 (MQ=255)
                         ttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATc                                                                      >  2:9848749/1‑96 (MQ=255)
                         ttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATc                                                                      <  1:14182976/96‑1 (MQ=255)
                          tctcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCg                                                                     >  2:409632/1‑96 (MQ=255)
                          tctcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCg                                                                     <  2:6342858/96‑1 (MQ=255)
                           ctcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGt                                                                    >  2:10471301/1‑96 (MQ=255)
                           ctcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCG‑GGCGCAGCGTGGATCCAGTTCCTGATCGt                                                                    <  2:13875836/96‑1 (MQ=255)
                            tcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTc                                                                   <  2:8481762/96‑1 (MQ=255)
                            tcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTc                                                                   <  2:9660833/96‑1 (MQ=255)
                             ccGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCa                                                                  >  1:5470173/1‑96 (MQ=255)
                                aaaTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATcc                                                               >  2:2076196/1‑96 (MQ=255)
                                 aaTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAATTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATccc                                                              <  2:12223491/96‑1 (MQ=255)
                                 aaTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATccc                                                              >  2:12037895/1‑96 (MQ=255)
                                  aTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCg                                                             <  1:199916/96‑1 (MQ=255)
                                  aTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCg                                                             >  2:3132481/1‑96 (MQ=255)
                                   tCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGc                                                            >  2:4875273/1‑96 (MQ=255)
                                   tCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGc                                                            >  1:6696519/1‑96 (MQ=255)
                                    cccGGAAGGCATCTTTGGGAAGAAAACCGTCGAGCTGAACGAAGACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCATGATCGTCATCCCGCt                                                           >  1:2495881/1‑96 (MQ=255)
                                     ccGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtg                                                          >  2:7246255/1‑96 (MQ=255)
                                     ccGGAAGGCATCTTTGCTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtg                                                          <  1:12789593/96‑1 (MQ=255)
                                       gtaaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCG‑GGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgat                                                        >  1:11867955/3‑96 (MQ=255)
                                       ggAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgat                                                        >  1:7619646/1‑96 (MQ=255)
                                        gAAGGCATCTTTGGTAAGAAAACCTTCGAACTTAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgatg                                                       <  2:10838211/96‑1 (MQ=255)
                                         aaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATGCCGCtgatga                                                      >  2:13191100/1‑96 (MQ=255)
                                            gCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAACGTCATCCCGCTGATGATTa                                                   >  1:1292683/1‑96 (MQ=255)
                                             cATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAt                                                  >  1:11564689/1‑96 (MQ=255)
                                              aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCGGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTGATCCCGCTGATGATTAT‑c                                                >  1:4288689/1‑96 (MQ=255)
                                              aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                >  2:7584079/1‑96 (MQ=255)
                                              aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                <  1:8045721/96‑1 (MQ=255)
                                                cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                              <  1:4881179/96‑2 (MQ=255)
                                                cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                              <  1:8291168/96‑2 (MQ=255)
                                                cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                              >  2:464517/1‑95 (MQ=255)
                                                cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                              <  2:9212153/96‑2 (MQ=255)
                                                  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                            >  2:14211096/1‑93 (MQ=255)
                                                   tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                           >  1:6474379/1‑92 (MQ=255)
                                                   tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                           <  1:4159130/96‑5 (MQ=255)
                                                      aaaGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                         >  1:11425702/2‑96 (MQ=255)
                                                       aaGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                        >  2:4241637/1‑96 (MQ=255)
                                                        aGAAACCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                       <  1:1559121/96‑1 (MQ=255)
                                                        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                       <  1:12180726/96‑1 (MQ=255)
                                                        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                       <  1:8079353/96‑1 (MQ=255)
                                                         gAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCgg                                      <  1:7328073/96‑1 (MQ=255)
                                                           aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGtt                                    <  2:4425450/96‑1 (MQ=255)
                                                             aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                                  <  2:8193610/96‑1 (MQ=255)
                                                             aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                                  <  2:157647/96‑1 (MQ=255)
                                                             aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                                  <  1:10728663/96‑1 (MQ=255)
                                                              ccGTCGACCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                 <  1:9292878/96‑1 (MQ=255)
                                                               cGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                                >  2:380554/1‑96 (MQ=255)
                                                                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                               <  2:7888738/96‑1 (MQ=255)
                                                                 tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                              <  2:9458105/96‑1 (MQ=255)
                                                                 tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                              <  1:9848749/96‑1 (MQ=255)
                                                                  cGACCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                             <  2:3370970/96‑1 (MQ=255)
                                                                   gAACTGTACGAAAACGGCGTTCCGGGGC‑GCGCAGGGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                            <  2:12785670/96‑1 (MQ=255)
                                                                   gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                            <  2:1483647/96‑1 (MQ=255)
                                                                   gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                            >  2:1176955/1‑96 (MQ=255)
                                                                    aaCTGAGCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCg                           <  2:2832490/96‑1 (MQ=255)
                                                                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                         >  1:3340033/1‑96 (MQ=255)
                                                                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                         <  2:11059345/96‑1 (MQ=255)
                                                                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                       <  2:11564689/96‑1 (MQ=255)
                                                                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                   >  1:2306895/1‑96 (MQ=255)
                                                                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                   <  2:5412848/96‑1 (MQ=255)
                                                                            gAAAACGCCGTTCCGCAGC‑GCGCAGCGCGGATCCAGTTCCTGATAGTCACCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                   <  2:12918397/96‑1 (MQ=255)
                                                                              aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                 <  1:9960655/96‑1 (MQ=255)
                                                                              aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                 >  1:8398505/1‑96 (MQ=255)
                                                                              aaaCGGCGTGCCGGCG‑AGCGCAGCGTCGATCCAGTTCCTGATCGTCATCTCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                 <  1:14219749/96‑1 (MQ=255)
                                                                               agcGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                <  2:1773946/94‑1 (MQ=255)
                                                                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                >  2:2655280/1‑96 (MQ=255)
                                                                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga               >  1:13628430/1‑96 (MQ=255)
                                                                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga               >  1:588887/1‑96 (MQ=255)
                                                                                  ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg             >  2:1135760/1‑96 (MQ=255)
                                                                                  ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg             >  1:6987591/1‑96 (MQ=255)
                                                                                  gacGTTCCGGCGC‑ACGCAGCATGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATCCCGTGCAGGATCtgatg             <  1:4004170/94‑1 (MQ=255)
                                                                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt          >  1:5133253/1‑96 (MQ=255)
                                                                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGCTCGTAATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGCATCTGATGAATa         <  1:6244540/96‑1 (MQ=255)
                                                                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa         >  2:6675614/1‑96 (MQ=255)
                                                                                         cggcgc‑gcgcAGCATGGATCCAGTTCCTGATCGGCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta      >  1:13409110/1‑96 (MQ=255)
                                                                                              tggcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat  >  1:11299060/2‑96 (MQ=255)
                                                                                               |                                                                                              
GACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAT  >  NC_000913/3224783‑3224971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 264 total aligned reads displayed.
TGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCG‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG  >  NC_000913/3224779‑3224978
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   nnncaGCAACGCCGGTGAAAATTTTCTTCTCCGAACTCCCGGAAGGCATCTTTGCTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCA                                                                                                  <  SRR4302518.8655417/96‑1 (MQ=60)
   GGACCGCAACGCCGGTGACAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCA                                                                                                  >  SRR4302518.5253359/1‑101 (MQ=60)
      NNNCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCG                                                                                               <  SRR4302518.8893178/101‑1 (MQ=60)
      CCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCG                                                                                               >  SRR4302518.1555195/1‑101 (MQ=60)
       CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGT                                                                                              >  SRR4302518.7111669/1‑101 (MQ=60)
        NNNACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTG                                                                                             <  SRR4302518.8466188/101‑1 (MQ=60)
        GCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTG                                                                                             <  SRR4302518.5362831/101‑1 (MQ=60)
        GCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTG                                                                                             <  SRR4302518.6187445/101‑1 (MQ=60)
         NNNCGCTGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGG                                                                                            <  SRR4302518.12571433/101‑1 (MQ=60)
         CAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGNNN                                                                                            >  SRR4302518.12578111/1‑101 (MQ=60)
          AACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGA                                                                                           >  SRR4302518.6444396/1‑101 (MQ=60)
           ACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGNNN                                                                                          >  SRR4302518.13668512/1‑101 (MQ=60)
           ACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGAT                                                                                          <  SRR4302518.5731859/101‑1 (MQ=60)
            NNNCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATT                                                                                         <  SRR4302518.9259011/101‑1 (MQ=60)
            CGCCGGTTAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATC                                                                                         <  SRR4302518.6475477/101‑1 (MQ=60)
            CGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTCAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATC                                                                                         <  SRR4302518.5299756/101‑1 (MQ=60)
             NNNGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCC                                                                                        <  SRR4302518.11484707/101‑1 (MQ=60)
             GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCC                                                                                        <  SRR4302518.5440259/101‑1 (MQ=60)
             GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCC                                                                                        >  SRR4302518.2751261/1‑101 (MQ=60)
             GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCC                                                                                        >  SRR4302518.471574/1‑101 (MQ=60)
             GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGGAAGAAAACCGTCGAACTGAACGAAAACGGCATTACGGCGC‑‑GCGCACCGTGGATCC                                                                                        >  SRR4302518.2599243/1‑101 (MQ=60)
              NNNGTGTAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCTGGCGC‑‑GCGCAGCGTGGATCCA                                                                                       <  SRR4302518.13457968/101‑1 (MQ=60)
              NNNGTGAAAATTTTCTTCTCCGAAGTCCCGGAAGGCATCTTTGGTAAGAAACCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCA                                                                                       <  SRR4302518.7499017/101‑1 (MQ=60)
              NNNGTGAAAATTTTCTTCTCCGAACTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCA                                                                                       <  SRR4302518.10477187/101‑1 (MQ=60)
              NNNGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCgc                                                                                       <  SRR4302518.12333818/101‑3 (MQ=60)
              NNNGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCA                                                                                       <  SRR4302518.9789109/101‑1 (MQ=60)
              NNNGTGAAAATTTTCTTCTCCGAAATCCAGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCA                                                                                       <  SRR4302518.8244529/101‑1 (MQ=60)
               CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAG                                                                                      >  SRR4302518.1869823/1‑101 (MQ=60)
               CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGACGTTCCGGCGC‑‑GCGCAGCGTGGATCNNN                                                                                      >  SRR4302518.10579217/1‑101 (MQ=60)
                GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCNNN                                                                                     >  SRR4302518.8008233/1‑101 (MQ=60)
                GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGT                                                                                     >  SRR4302518.335777/1‑101 (MQ=60)
                AGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCNNN                                                                                     >  SRR4302518.11808536/1‑101 (MQ=60)
                 NNNAAAATTTTCTTCTCCGACATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTT                                                                                    <  SRR4302518.14357161/101‑1 (MQ=60)
                 NNNAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTT                                                                                    <  SRR4302518.8495968/101‑1 (MQ=60)
                 GTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTT                                                                                    <  SRR4302518.1181366/101‑1 (MQ=60)
                 GAGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGGGGATCCgnnn                                                                                    >  SRR4302518.12056762/1‑97 (MQ=60)
                  TGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGNNN                                                                                   >  SRR4302518.10140506/1‑101 (MQ=60)
                   NNNAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCC                                                                                  <  SRR4302518.12427161/101‑1 (MQ=60)
                   GAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCTAGTNNN                                                                                  >  SRR4302518.13486914/1‑101 (MQ=60)
                   GAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCC                                                                                  <  SRR4302518.4624912/101‑1 (MQ=60)
                     ACATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCNNN                                                                                >  SRR4302518.8147328/1‑101 (MQ=60)
                     AAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTG                                                                                >  SRR4302518.5562962/1‑101 (MQ=60)
                     AAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCAAGTTCCTG                                                                                >  SRR4302518.4841899/1‑101 (MQ=60)
                      AATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCNNN                                                                               >  SRR4302518.10106141/1‑101 (MQ=60)
                       agtgTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAATCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGAA                                                                              <  SRR4302518.4394894/97‑1 (MQ=60)
                       ATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTNNN                                                                              >  SRR4302518.8888580/1‑101 (MQ=60)
                        TTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACTGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                             <  SRR4302518.1432892/101‑1 (MQ=60)
                        TTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGNNN                                                                             >  SRR4302518.10658274/1‑101 (MQ=60)
                        TTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGNNN                                                                             >  SRR4302518.10773529/1‑101 (MQ=60)
                        NNNTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                             <  SRR4302518.14833764/101‑1 (MQ=60)
                        NNNTCTACTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                             <  SRR4302518.13477981/101‑1 (MQ=60)
                         TTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGANNN                                                                            >  SRR4302518.15011789/1‑101 (MQ=60)
                         TTTCTGCTCCGAAATGCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCG                                                                            <  SRR4302518.6646721/101‑1 (MQ=60)
                         NNNCTTCTTCGAAATGCCGGAAGACATCTTAGGTAAGAAAACCGTCGAACTGACCGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCT                                                                            <  SRR4302518.9362645/101‑1 (MQ=60)
                           TCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTC                                                                          >  SRR4302518.380575/1‑101 (MQ=60)
                            NNNCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCA                                                                         <  SRR4302518.12613221/101‑1 (MQ=60)
                            CTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGGACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGNNN                                                                         >  SRR4302518.13612369/1‑101 (MQ=60)
                            CTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGAGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGNNN                                                                         >  SRR4302518.13726750/1‑101 (MQ=60)
                             TGCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT                                                                        <  SRR4302518.3900793/101‑1 (MQ=60)
                             NNNTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCCT                                                                        <  SRR4302518.14925537/101‑1 (MQ=60)
                              TCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCNNN                                                                       >  SRR4302518.14306930/1‑101 (MQ=60)
                              TCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTGCCTGATCGTCATC                                                                       >  SRR4302518.1490367/1‑101 (MQ=60)
                              NNNCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATC                                                                       <  SRR4302518.11568907/101‑1 (MQ=60)
                              NNNCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATC                                                                       <  SRR4302518.13075849/101‑1 (MQ=60)
                               NNNCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCC                                                                      <  SRR4302518.14206654/101‑1 (MQ=60)
                               CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCC                                                                      >  SRR4302518.1038672/1‑101 (MQ=60)
                               CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCANNN                                                                      >  SRR4302518.9636347/1‑101 (MQ=60)
                               CCCCGAAATCCCGGAAGGCATCTTTGGTAAGACAACCGTCGAACTTAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGACCCAGTTCCTGCTCGTCATCT                                                                      <  SRR4302518.5734932/101‑1 (MQ=60)
                                TCCGAAATCCCGGAAGGCAGCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGACGC‑‑GCGCAGCGTGGATCATGTTCCTGATCGTCATCTC                                                                     >  SRR4302518.3590751/1‑101 (MQ=60)
                                 CCGACATCCCGGAAGGCATCGTTGGTAAGCAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGCTCCAGTTCCTGATCGTCATCCCG                                                                    <  SRR4302518.3612482/101‑1 (MQ=60)
                                 CCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCG                                                                    >  SRR4302518.5748739/1‑101 (MQ=60)
                                 CCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGGTCCGGCGC‑‑GCGCAGCGTGGGTCCAGTTCCTGATCGTCtgcnnn                                                                    >  SRR4302518.10857896/1‑95 (MQ=60)
                                  NNNAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGC                                                                   <  SRR4302518.8449793/101‑1 (MQ=60)
                                  CGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCNNN                                                                   >  SRR4302518.10056948/1‑101 (MQ=60)
                                  CGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCNNN                                                                   >  SRR4302518.8707455/1‑101 (MQ=60)
                                   GAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCNNN                                                                  >  SRR4302518.8680765/1‑101 (MQ=60)
                                    NNNTCCCGGAAGGCATCTTTGCTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                                 <  SRR4302518.13385280/101‑1 (MQ=60)
                                     AAGCCCGGAAGCCATCTTTGGTACGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGA                                                                <  SRR4302518.6971588/101‑1 (MQ=60)
                                      ATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                               >  SRR4302518.978336/1‑101 (MQ=60)
                                      ATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                               >  SRR4302518.5003758/1‑101 (MQ=60)
                                       TCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATG                                                              >  SRR4302518.7043549/1‑101 (MQ=60)
                                        CCCGGAAGGCATCTTTGGGAAGAAAACCGTCGAGCTGAACGAAGACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCATGATCGTCATCCCGCTGAGGA                                                             >  SRR4302518.2616152/1‑101 (MQ=60)
                                         CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAT                                                            >  SRR4302518.4398409/1‑101 (MQ=60)
                                          NNNAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAACCGGGGTTCCGGCGC‑‑GCGCAGCGTGGCTCCAGTTCCTGATCGTCATCCCGCTGATGATT                                                           <  SRR4302518.14681954/101‑1 (MQ=60)
                                          CGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATT                                                           <  SRR4302518.6060730/101‑1 (MQ=60)
                                           GTAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCG‑G‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGANNN                                                          >  SRR4302518.12432963/1‑101 (MQ=60)
                                           GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA                                                          <  SRR4302518.1347724/101‑1 (MQ=60)
                                           GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGANNN                                                          >  SRR4302518.8008453/1‑101 (MQ=60)
                                           GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCGGATCGTCATCCCGCTGATGATTA                                                          >  SRR4302518.3163983/1‑101 (MQ=60)
                                            GAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGTTCCAGTTCCTGATCGTCATCCCGCTGATGATTAT                                                         <  SRR4302518.6454395/101‑1 (MQ=60)
                                            GAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATNNN                                                         >  SRR4302518.14499866/1‑101 (MQ=60)
                                             NNNGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C                                                       <  SRR4302518.8446220/101‑1 (MQ=60)
                                             AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGACAGTCATCCCGCTGATGATTNN‑N                                                       >  SRR4302518.13393297/1‑101 (MQ=60)
                                              NNNCATCGTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTGCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑GC                                                      <  SRR4302518.14936325/101‑1 (MQ=60)
                                              AGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC                                                      <  SRR4302518.5250389/101‑1 (MQ=60)
                                               NNNATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCT                                                     <  SRR4302518.11975211/101‑1 (MQ=60)
                                               NNNATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCC                                                     <  SRR4302518.8699585/101‑1 (MQ=60)
                                               GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCC                                                     <  SRR4302518.5135907/101‑1 (MQ=60)
                                                NNNTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGG                                                    <  SRR4302518.12346848/101‑1 (MQ=60)
                                                GCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGAACGTCATCCCGCTGATGATTAT‑CCcg                                                    >  SRR4302518.1345714/1‑99 (MQ=60)
                                                GCATATTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                                    <  SRR4302518.3521723/101‑3 (MQ=60)
                                                 NNNCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                                   <  SRR4302518.12204353/101‑4 (MQ=60)
                                                 CATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCNNN                                                   >  SRR4302518.12109016/1‑101 (MQ=60)
                                                 CATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCNNN                                                   >  SRR4302518.9208569/1‑101 (MQ=60)
                                                  ATCTTTGGTAAGAACACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGAT                                                   <  SRR4302518.3230639/101‑1 (MQ=60)
                                                  ATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCGGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTGATCCCGCTGATGATTATCCCGAT                                                   >  SRR4302518.4501019/1‑101 (MQ=60)
                                                   TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATG                                                  >  SRR4302518.6732534/1‑101 (MQ=60)
                                                   NNNTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATG                                                  <  SRR4302518.13770599/101‑1 (MQ=60)
                                                    CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAACGTGGATCCATTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                                 <  SRR4302518.7310711/101‑1 (MQ=60)
                                                      TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTC                                               <  SRR4302518.2660465/101‑1 (MQ=60)
                                                      TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTC                                               <  SRR4302518.2173098/101‑1 (MQ=60)
                                                       TGGTAAGAAACCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                              <  SRR4302518.1626516/101‑1 (MQ=60)
                                                       TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                              >  SRR4302518.6805793/1‑101 (MQ=60)
                                                       TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcgatgcnnn                                             >  SRR4302518.14851640/1‑92 (MQ=60)
                                                       NNNTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                              <  SRR4302518.12758592/101‑1 (MQ=60)
                                                        NNNAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGG                                             <  SRR4302518.7709073/101‑1 (MQ=60)
                                                        GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGTTGATTATCCCGTTGCTCGG                                             >  SRR4302518.6783691/1‑101 (MQ=60)
                                                        GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGG                                             <  SRR4302518.2606756/101‑1 (MQ=60)
                                                         NNNAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGCT                                            <  SRR4302518.14974599/101‑1 (MQ=60)
                                                         GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGT                                            >  SRR4302518.2138744/1‑101 (MQ=60)
                                                         GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGT                                            >  SRR4302518.4277328/1‑101 (MQ=60)
                                                          TAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGNNN                                           >  SRR4302518.12082115/1‑101 (MQ=60)
                                                          AAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGNNN                                           >  SRR4302518.11960818/1‑101 (MQ=60)
                                                            NNNAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCC                                         <  SRR4302518.11232701/101‑1 (MQ=60)
                                                            AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCC                                         <  SRR4302518.4340707/101‑1 (MQ=60)
                                                            AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTNNN                                         >  SRR4302518.12562766/1‑101 (MQ=60)
                                                             NNNAACCGTCGACCTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCA                                        <  SRR4302518.9741195/101‑1 (MQ=60)
                                                              AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCNNN                                       >  SRR4302518.14821264/1‑101 (MQ=60)
                                                              AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAA                                       >  SRR4302518.2665626/1‑101 (MQ=60)
                                                                AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATTCTCGGTTCCAATA                                     >  SRR4302518.1823676/1‑101 (MQ=60)
                                                                AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATA                                     <  SRR4302518.4952235/101‑1 (MQ=60)
                                                                 ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAC                                    <  SRR4302518.4449932/101‑1 (MQ=60)
                                                                 ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCACGCTGATGATTATCCCGATGCTCGGTTCCAANNN                                    >  SRR4302518.10322726/1‑101 (MQ=60)
                                                                   CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATANNN                                  >  SRR4302518.7632502/1‑101 (MQ=60)
                                                                   CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAGTATCCCGATGCTCTGTTCCAATACCG                                  >  SRR4302518.3420844/1‑101 (MQ=60)
                                                                   CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCACAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATCCCG                                  <  SRR4302518.3560109/101‑1 (MQ=60)
                                                                    GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACNNN                                 >  SRR4302518.12068134/1‑101 (MQ=60)
                                                                     TCGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTG                                <  SRR4302518.3373182/101‑1 (MQ=60)
                                                                      CGAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGC                               <  SRR4302518.30653/101‑1 (MQ=60)
                                                                       caggctggaaggtgaggaaagcgacctcgcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCNNN                                >  SRR4302518.14013814/31‑101 (MQ=60)
                                                                       NNNCTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                              <  SRR4302518.8328047/101‑1 (MQ=60)
                                                                       NNNCTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                              <  SRR4302518.12966868/101‑1 (MQ=60)
                                                                       GAACTGAACGAACACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                              >  SRR4302518.5375982/1‑101 (MQ=60)
                                                                       GAACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTNNN                              >  SRR4302518.10384976/1‑101 (MQ=60)
                                                                        AACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                             <  SRR4302518.2472692/101‑1 (MQ=60)
                                                                         NNNGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                            <  SRR4302518.12611161/101‑1 (MQ=60)
                                                                         ACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                            <  SRR4302518.870681/101‑1 (MQ=60)
                                                                         ACTGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                            <  SRR4302518.4256884/101‑1 (MQ=60)
                                                                         ACTGAAAGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGGGGATCCAGTTCCTGATCGCCATCCCGCTGATGATGATACCGTTGCTCGGTTCCACTACCGTGCNNN                            >  SRR4302518.14164938/1‑101 (MQ=58)
                                                                          NNNAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGCA                           <  SRR4302518.13665133/101‑1 (MQ=60)
                                                                           TGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGNNN                          >  SRR4302518.9503964/1‑101 (MQ=60)
                                                                           TGAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAT                          <  SRR4302518.3511794/101‑1 (MQ=60)
                                                                            GCACGAGAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATC                         <  SRR4302518.1247374/101‑1 (MQ=60)
                                                                            GAACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGNNN                         >  SRR4302518.7835698/1‑101 (MQ=60)
                                                                             NNNGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                        <  SRR4302518.10434973/101‑1 (MQ=60)
                                                                             NNNGAAAACGGCGTGCCGGCG‑A‑GCGCAGCGTCGATCCAGTTCCTGATCGTCATCTCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                        <  SRR4302518.14873663/101‑1 (MQ=60)
                                                                             AACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGANNN                        >  SRR4302518.7361513/1‑101 (MQ=60)
                                                                              NNNAAAACGGCGTTCCGGCGCT‑G‑GCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG                       <  SRR4302518.13414081/101‑1 (MQ=60)
                                                                              ACGAAACCGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG                       <  SRR4302518.1335385/101‑1 (MQ=60)
                                                                              ACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG                       >  SRR4302518.6976111/1‑101 (MQ=60)
                                                                              ACGAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG                       >  SRR4302518.3694794/1‑101 (MQ=60)
                                                                                GAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGAT                     >  SRR4302518.2419126/1‑101 (MQ=60)
                                                                                GAAAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGAT                     >  SRR4302518.4817553/1‑101 (MQ=60)
                                                                                 nnntgGGCGTTCCGGCGC‑‑GCGCAGCGTGGCTCCAGTTCCTGATCGTCCTCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                    <  SRR4302518.10111467/96‑1 (MQ=60)
                                                                                 NNNACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                    <  SRR4302518.10416569/101‑1 (MQ=60)
                                                                                  AAACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGANNN                   >  SRR4302518.8810318/1‑101 (MQ=60)
                                                                                   AGCGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATNNN                  >  SRR4302518.12338536/1‑101 (MQ=60)
                                                                                   ACCGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA                  <  SRR4302518.4398608/101‑1 (MQ=60)
                                                                                   AACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATNNN                  >  SRR4302518.14281324/1‑101 (MQ=60)
                                                                                   AACGGCGTACCGGAGC‑‑GCGCAGCCTGCATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA                  <  SRR4302518.1816577/101‑1 (MQ=60)
                                                                                    ACGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                 >  SRR4302518.611776/1‑101 (MQ=60)
                                                                                     CGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGCTCGTAATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGCATCTGATGAATA                <  SRR4302518.6562939/101‑1 (MQ=60)
                                                                                     CGGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA                <  SRR4302518.4609312/101‑1 (MQ=60)
                                                                                      GGCGTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAANNN               >  SRR4302518.7358834/1‑101 (MQ=60)
                                                                                         NNNCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATATGATGAATACTAT            <  SRR4302518.14065497/101‑1 (MQ=60)
                                                                                         GTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACNNN            >  SRR4302518.15014963/1‑101 (MQ=60)
                                                                                         GTTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACNNN            >  SRR4302518.13886302/1‑101 (MQ=60)
                                                                                          TTCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT           >  SRR4302518.6006088/1‑101 (MQ=60)
                                                                                           TCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAGCGTGCAGGATCTGATGAATACTATTA          >  SRR4302518.3846840/1‑101 (MQ=60)
                                                                                           TCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGGATACTATTA          >  SRR4302518.2325177/1‑101 (MQ=60)
                                                                                           TCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA          <  SRR4302518.5514945/101‑1 (MQ=60)
                                                                                           TCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA          <  SRR4302518.6939878/101‑1 (MQ=60)
                                                                                           TCCGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA          <  SRR4302518.837651/101‑1 (MQ=60)
                                                                                             nnnggcgcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA          <  SRR4302518.14205496/93‑1 (MQ=60)
                                                                                             CGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT        <  SRR4302518.2508113/101‑1 (MQ=60)
                                                                                             CGGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT        >  SRR4302518.6645373/1‑101 (MQ=60)
                                                                                             CGGCGC‑‑GCGCAGCATGGATCCAGTTCCTGATCGGCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTNNN        >  SRR4302518.14025608/1‑101 (MQ=60)
                                                                                              ccggagcGCGCAGCGTGGCTCCAGTTCCTGATCGTCATCGCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAACACTATNNN         >  SRR4302518.13220339/8‑101 (MQ=60)
                                                                                              GGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTANNN       >  SRR4302518.13847359/1‑101 (MQ=60)
                                                                                              GGCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGCGCAGGATCTGATGAATACTATTATTA       >  SRR4302518.2001585/1‑101 (MQ=60)
                                                                                               GCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA      >  SRR4302518.1055836/1‑101 (MQ=60)
                                                                                               GCGC‑‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA      <  SRR4302518.1819589/101‑1 (MQ=60)
                                                                                                CGCGCGCGCAGCGTGGATCAAGTTCCTGATCGTCACCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTA       <  SRR4302518.907678/101‑1 (MQ=60)
                                                                                                 NNN‑GCGCAGCGTGGATCCAGTTCCTGATAGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT     <  SRR4302518.10704486/101‑1 (MQ=60)
                                                                                                  TG‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTANNN    >  SRR4302518.11828225/1‑101 (MQ=60)
                                                                                                   gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA    >  SRR4302518.4957064/3‑101 (MQ=60)
                                                                                                   C‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT   <  SRR4302518.2044638/101‑1 (MQ=60)
                                                                                                     NNNCAGCCTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGCTGAATACTATTATTAATATG  <  SRR4302518.15086973/101‑1 (MQ=60)
                                                                                                     GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATNNN  >  SRR4302518.7702801/1‑101 (MQ=60)
                                                                                                     GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG  <  SRR4302518.2882551/101‑1 (MQ=60)
                                                                                                     |                                                                                                    
TGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCG‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG  >  NC_000913/3224779‑3224978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑