Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	763,352	A→G	K372K (AAA→AAG)	sucC →	succinyl‑CoA synthetase beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	763,352	0	A	G	99.5%	719.7 / ‑4.5	207	K372K (AAA→AAG)	sucC	succinyl‑CoA synthetase beta subunit
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (84/122); total (85/122)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01

Only 100 of 220 total aligned reads displayed.

CGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGC  >  NC_000913/763262‑763441

cGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAggg                                                                                         <  1:3227374/93‑1 (MQ=255)

cGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAggg                                                                                         >  2:1949294/1‑93 (MQ=255)

cGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAggg                                                                                         >  2:2305055/1‑93 (MQ=255)

 gTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGt                                                                                        >  1:2746078/1‑93 (MQ=255)

  tACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTc                                                                                       <  2:2602654/93‑1 (MQ=255)

   tccGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCt                                                                                      <  2:4182314/92‑1 (MQ=255)

   aCCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCt                                                                                      >  2:5245175/1‑93 (MQ=255)

   aCCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCt                                                                                      <  2:1171832/93‑1 (MQ=255)

   aCCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCt                                                                                      <  2:2346638/93‑1 (MQ=255)

    ccGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTg                                                                                     >  2:597351/1‑93 (MQ=255)

    ccGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTg                                                                                     <  1:560365/93‑1 (MQ=255)

    ccGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTg                                                                                     <  2:375926/93‑1 (MQ=255)

     cGGTCGTTGTACGTCTGGAAGGTAACAACGCCGTACGCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGa                                                                                    <  1:2678934/93‑1 (MQ=255)

      ggTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGAc                                                                                   >  2:257033/1‑93 (MQ=255)

      ggTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGAc                                                                                   >  1:3484227/1‑93 (MQ=255)

       gTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACg                                                                                  >  1:5006834/1‑93 (MQ=255)

         cGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGa                                                                                <  1:757415/93‑1 (MQ=255)

         cGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGa                                                                                <  1:4947830/93‑1 (MQ=255)

          gTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGAAGCAAAGGGTTTGACGGAt                                                                               <  1:6316360/93‑1 (MQ=255)

           tGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATg                                                                              <  2:5014861/93‑1 (MQ=255)

              tACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAg                                                                           <  1:2827125/93‑1 (MQ=255)

                 gTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTc                                                                        <  1:1477857/93‑1 (MQ=255)

                 gTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTc                                                                        >  1:5048373/1‑93 (MQ=255)

                      gAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTcagcag                                                                   >  1:1132744/1‑93 (MQ=255)

                        aGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGgt                                                                 <  2:3509368/93‑1 (MQ=255)

                        aGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGgt                                                                 <  1:400459/93‑1 (MQ=255)

                        aGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGgt                                                                 >  2:2628524/1‑93 (MQ=255)

                         ggTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGgtt                                                                <  1:3684605/93‑1 (MQ=255)

                           tAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGgttgt                                                              >  2:4846712/1‑93 (MQ=255)

                               aacGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGcc                                                          >  1:5184051/1‑93 (MQ=255)

                                 cGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGc                                                        >  1:4229109/1‑93 (MQ=255)

                                 cGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGc                                                        >  2:5116563/1‑93 (MQ=255)

                                 cGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGc                                                        <  2:6071095/93‑1 (MQ=255)

                                    cGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGGCTGACGGAGCCAGCTCAGCAAGTTGGTGCGGCAGt                                                     >  2:668247/1‑93 (MQ=255)

                                     gAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTg                                                    <  1:767037/93‑1 (MQ=255)

                                     gAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTg                                                    <  2:3188288/93‑1 (MQ=255)

                                      aaCTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTgg                                                   >  2:2427152/1‑93 (MQ=255)

                                       aCTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGa                                                  <  1:5788050/93‑1 (MQ=255)

                                        cTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAg                                                 <  2:5373133/93‑1 (MQ=255)

                                         tCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAgg                                                >  2:4298289/1‑93 (MQ=255)

                                         tCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAgg                                                <  2:4667957/93‑1 (MQ=255)

                                          cGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAggg                                               <  1:4652016/93‑1 (MQ=255)

                                          cGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAggg                                               >  1:4418696/1‑93 (MQ=255)

                                            gcgcGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGa                                             >  2:5717077/1‑93 (MQ=255)

                                            gcgcGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGa                                             <  2:3141081/93‑1 (MQ=255)

                                            gcgcGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGa                                             >  1:1308379/1‑93 (MQ=255)

                                              gcgAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGaaa                                           >  1:2375604/1‑93 (MQ=255)

                                               cgAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAaat                                          <  1:4364665/93‑1 (MQ=255)

                                                 aagaaACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGGaataa                                        >  1:2498598/1‑93 (MQ=255)

                                                 aagaaACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAaataa                                        <  2:1412485/93‑1 (MQ=255)

                                                 aagaaACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAaataa                                        >  2:6307899/1‑93 (MQ=255)

                                                 aagaaACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGAGGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAaataa                                        >  2:4124750/1‑93 (MQ=255)

                                                  agaaACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAaataat                                       <  2:2106232/93‑1 (MQ=255)

                                                   gaaACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATg                                      >  1:4750514/1‑93 (MQ=255)

                                                    aaaCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGt                                     <  1:1693227/93‑1 (MQ=255)

                                                    aaaCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGt                                     >  2:62040/1‑93 (MQ=255)

                                                    aaaCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGt                                     >  2:4016994/1‑93 (MQ=255)

                                                    aaaCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGt                                     >  2:4758174/1‑93 (MQ=255)

                                                     aaCTGGCTGAAAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTc                                    >  1:3931971/1‑93 (MQ=255)

                                                      aCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTcc                                   <  2:101499/93‑1 (MQ=255)

                                                      aCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTcc                                   >  1:267269/1‑93 (MQ=255)

                                                      aCTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTcc                                   <  1:2962517/93‑1 (MQ=255)

                                                       cTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCa                                  <  1:218509/93‑1 (MQ=255)

                                                        tGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAt                                 <  1:2209464/93‑1 (MQ=255)

                                                        tGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAt                                 <  1:586609/93‑1 (MQ=255)

                                                         ggCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAtt                                <  1:4368470/93‑1 (MQ=255)

                                                         ggCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAtt                                <  2:1082171/93‑1 (MQ=255)

                                                         ggCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAtt                                >  1:4316702/1‑93 (MQ=255)

                                                           cTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAtttt                              >  1:3723809/1‑93 (MQ=255)

                                                            tGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTa                             >  1:1967286/1‑93 (MQ=255)

                                                            tGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTa                             >  1:4154993/1‑93 (MQ=255)

                                                             gACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTaa                            <  2:2666434/93‑1 (MQ=255)

                                                              aCAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAAt                           <  2:6404658/93‑1 (MQ=255)

                                                              aCAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAAt                           >  2:2899358/1‑93 (MQ=255)

                                                               cAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGTGATAATGTCCATTTTAATc                          >  1:1404549/1‑93 (MQ=255)

                                                               cAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATc                          >  2:3171397/1‑93 (MQ=255)

                                                                 gCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGa                        >  2:3720166/1‑93 (MQ=255)

                                                                 gCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGa                        <  2:1556537/93‑1 (MQ=255)

                                                                  cGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGAt                       <  1:3283431/93‑1 (MQ=255)

                                                                    gCCTGAATATGATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGGGGGGAAATAATGTCCATTTTAATCGATaa                     <  2:5771852/93‑1 (MQ=255)

                                                                      cTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATaaaa                   >  1:2841682/1‑93 (MQ=255)

                                                                      cTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATaaaa                   >  1:5302306/1‑93 (MQ=255)

                                                                       tGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATaaaaa                  >  2:1787224/1‑93 (MQ=255)

                                                                        gAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAac                 <  2:1224489/93‑1 (MQ=255)

                                                                         aaTATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAaca                >  2:5257850/1‑93 (MQ=255)

                                                                          atatTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAacac               >  1:2539706/1‑93 (MQ=255)

                                                                           tattatTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAGATAATGTCCATTTTAATCGATAAAAACAcc              >  2:6369281/1‑93 (MQ=255)

                                                                           tattatTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACAcc              <  2:497750/93‑1 (MQ=255)

                                                                              tattGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAg           <  1:4059585/93‑1 (MQ=255)

                                                                               attGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAgg          >  2:4483627/1‑93 (MQ=255)

                                                                                ttGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGt         <  2:5072902/93‑1 (MQ=255)

                                                                                ttGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGt         >  1:3595031/1‑93 (MQ=255)

                                                                                ttGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGt         <  1:1059336/93‑1 (MQ=255)

                                                                                ttGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGt         >  2:1494737/1‑93 (MQ=255)

                                                                                  gcagcaAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTa       >  2:701835/1‑93 (MQ=255)

                                                                                   cagcaAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTAt      >  2:3983490/1‑93 (MQ=255)

                                                                                   cagcaAAGGGTCTGACGAATGCAGCTCAGCAGGTTGTTGCCGCAGTGAAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTAt      <  1:1755168/93‑1 (MQ=255)

                                                                                    agcaAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATc     <  2:3362840/93‑1 (MQ=255)

                                                                                     gcaAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCt    <  2:456736/93‑1 (MQ=255)

                                                                                       aaagGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGc  <  1:3153186/93‑1 (MQ=255)

CGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGC  >  NC_000913/763262‑763441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGG  >  NC_000913/763259‑763445

TAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCTGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAG                                                                                               <  SRR4302534.3618774/94‑1 (MQ=60)

TAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAG                                                                                               >  SRR4302534.56118/1‑94 (MQ=60)

TAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAG                                                                                               >  SRR4302534.5626261/1‑94 (MQ=60)

 AACGTACCGGTCGTGTTACGTCTGGAAGGTAACAACGCCGAGCTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGG                                                                                              <  SRR4302534.5197241/94‑1 (MQ=60)

 AACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGG                                                                                              <  SRR4302534.3859547/94‑1 (MQ=60)

  ACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGG                                                                                             >  SRR4302534.3349804/1‑94 (MQ=60)

  ACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGG                                                                                             <  SRR4302534.2047842/94‑1 (MQ=60)

   CGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGT                                                                                            <  SRR4302534.1672592/94‑1 (MQ=60)

   CGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGT                                                                                            <  SRR4302534.3313119/94‑1 (MQ=60)

   CGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGT                                                                                            >  SRR4302534.2818406/1‑94 (MQ=60)

    GTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTC                                                                                           <  SRR4302534.1414178/94‑1 (MQ=60)

     TACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCT                                                                                          <  SRR4302534.1715262/94‑1 (MQ=60)

      ACCGGTCGTGGTACGTCTGGAAGGTAACAACGACGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTG                                                                                         <  SRR4302534.699209/94‑1 (MQ=60)

       CCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGA                                                                                        <  SRR4302534.5156454/94‑1 (MQ=60)

       CCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGA                                                                                        <  SRR4302534.574161/94‑1 (MQ=60)

       CCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGA                                                                                        <  SRR4302534.2491694/94‑1 (MQ=60)

        CGGTCGTTGTACGTCTGGAAGGTAACAACGCCGTACGCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGAC                                                                                       <  SRR4302534.2749752/94‑1 (MQ=60)

        CGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGAC                                                                                       >  SRR4302534.3577891/1‑94 (MQ=60)

         GGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACG                                                                                      >  SRR4302534.3254931/1‑94 (MQ=60)

         GGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACG                                                                                      >  SRR4302534.5142165/1‑94 (MQ=60)

          GTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGG                                                                                     <  SRR4302534.2576609/94‑1 (MQ=60)

          GTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGG                                                                                     >  SRR4302534.4323318/1‑94 (MQ=60)

           TCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGA                                                                                    >  SRR4302534.2946518/1‑94 (MQ=60)

            CGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGAT                                                                                   >  SRR4302534.5113723/1‑94 (MQ=60)

            CGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGAT                                                                                   <  SRR4302534.776203/94‑1 (MQ=60)

            CGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGAT                                                                                   <  SRR4302534.5081851/94‑1 (MQ=60)

             GTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATG                                                                                  <  SRR4302534.1464900/94‑1 (MQ=60)

             GTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAACAAAGGGTCTGACGGATG                                                                                  <  SRR4302534.784787/94‑1 (MQ=60)

             GTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGAAGCAAAGGGTTTGACGGATG                                                                                  <  SRR4302534.6487071/94‑1 (MQ=60)

              TGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGC                                                                                 >  SRR4302534.447917/1‑94 (MQ=60)

               GGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCA                                                                                >  SRR4302534.5848256/1‑94 (MQ=60)

                 TACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGC                                                                              <  SRR4302534.2901438/94‑1 (MQ=60)

                   CGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTC                                                                            >  SRR4302534.5184683/1‑94 (MQ=60)

                    GTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCA                                                                           <  SRR4302534.1516721/94‑1 (MQ=60)

                       TGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCA                                                                        >  SRR4302534.2743624/1‑94 (MQ=60)

                        GGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAG                                                                       >  SRR4302534.1162359/1‑94 (MQ=60)

                        GGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAG                                                                       <  SRR4302534.1777552/94‑1 (MQ=60)

                           AGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTT                                                                    <  SRR4302534.410697/94‑1 (MQ=60)

                            GGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTG                                                                   <  SRR4302534.3783672/94‑1 (MQ=60)

                            GGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTG                                                                   <  SRR4302534.5185667/94‑1 (MQ=60)

                                 CAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCC                                                              >  SRR4302534.5323778/1‑94 (MQ=60)

                                   ACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGC                                                            >  SRR4302534.4343071/1‑94 (MQ=60)

                                       CGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTG                                                        >  SRR4302534.2911832/1‑94 (MQ=60)

                                       CGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTG                                                        <  SRR4302534.5993166/94‑1 (MQ=60)

                                        GAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGG                                                       <  SRR4302534.786103/94‑1 (MQ=60)

                                          ACTCGGCGCTAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAG                                                     <  SRR4302534.2873009/94‑1 (MQ=60)

                                          ACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAG                                                     <  SRR4302534.5945061/94‑1 (MQ=60)

                                           CTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGG                                                    <  SRR4302534.3238392/94‑1 (MQ=60)

                                            TCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGG                                                   <  SRR4302534.228921/94‑1 (MQ=60)

                                            TCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGG                                                   >  SRR4302534.4537989/1‑94 (MQ=60)

                                             CGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGG                                                  <  SRR4302534.4778310/94‑1 (MQ=60)

                                              GGCGCGAAGAAACTGGCTGAGAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGGAGCTCAGCAGGTTGTTGTCGCAGTGGAGGGGA                                                 >  SRR4302534.3176917/1‑94 (MQ=60)

                                              GGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGA                                                 >  SRR4302534.1342894/1‑94 (MQ=60)

                                               tgcggcaacAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAA                                                >  SRR4302534.999473/10‑94 (MQ=60)

                                               GCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAA                                                <  SRR4302534.6327015/94‑1 (MQ=60)

                                               GCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAA                                                <  SRR4302534.4117272/94‑1 (MQ=60)

                                               GCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAA                                                <  SRR4302534.6179269/94‑1 (MQ=60)

                                                CGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAA                                               >  SRR4302534.2438686/1‑94 (MQ=60)

                                                  CGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATA                                             <  SRR4302534.4482347/94‑1 (MQ=60)

                                                   GAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGGAATAA                                            >  SRR4302534.2564950/1‑94 (MQ=60)

                                                    AAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGCTGCCGCAGTGGTGGGGAAATAAT                                           >  SRR4302534.3409034/1‑94 (MQ=60)

                                                     AGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATG                                          >  SRR4302534.4879470/1‑94 (MQ=60)

                                                      GAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGT                                         <  SRR4302534.917554/94‑1 (MQ=60)

                                                       AAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGGAATAATGTC                                        >  SRR4302534.4669942/1‑94 (MQ=60)

                                                       AAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTC                                        <  SRR4302534.1737230/94‑1 (MQ=60)

                                                       AAACTGGCTGAAAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTC                                        >  SRR4302534.4037294/1‑94 (MQ=60)

                                                        AACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCC                                       >  SRR4302534.274199/1‑94 (MQ=60)

                                                         ACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCA                                      <  SRR4302534.3040113/94‑1 (MQ=60)

                                                          CTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAT                                     <  SRR4302534.224239/94‑1 (MQ=60)

                                                          CTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAT                                     <  SRR4302534.1854490/94‑1 (MQ=60)

                                                           TGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATT                                    <  SRR4302534.4917821/94‑1 (MQ=60)

                                                           TGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATT                                    <  SRR4302534.2267959/94‑1 (MQ=60)

                                                           TGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATT                                    >  SRR4302534.4433593/1‑94 (MQ=60)

                                                           TGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATT                                    <  SRR4302534.4773934/94‑1 (MQ=60)

                                                           TGGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATT                                    <  SRR4302534.600936/94‑1 (MQ=60)

                                                            GGCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTT                                   <  SRR4302534.4486205/94‑1 (MQ=60)

                                                             GCTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTT                                  >  SRR4302534.3823896/1‑94 (MQ=60)

                                                              CTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTA                                 >  SRR4302534.4267173/1‑94 (MQ=60)

                                                              CTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTA                                 >  SRR4302534.2018782/1‑94 (MQ=60)

                                                              CTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTA                                 <  SRR4302534.266914/94‑1 (MQ=60)

                                                              CTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTA                                 <  SRR4302534.4447848/94‑1 (MQ=60)

                                                              CTGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTA                                 <  SRR4302534.1287754/94‑1 (MQ=60)

                                                               TGACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAA                                >  SRR4302534.5645675/1‑94 (MQ=60)

                                                                GACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAAT                               <  SRR4302534.2349657/94‑1 (MQ=60)

                                                                 ACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGTGATAATGTCCATTTTAATC                              >  SRR4302534.1441488/1‑94 (MQ=60)

                                                                 ACAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATC                              >  SRR4302534.5243719/1‑94 (MQ=60)

                                                                  CAGCGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCG                             <  SRR4302534.2768425/94‑1 (MQ=60)

                                                                     CGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATA                          <  SRR4302534.3370947/94‑1 (MQ=60)

                                                                     CGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATA                          <  SRR4302534.3813575/94‑1 (MQ=60)

                                                                     CGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATA                          <  SRR4302534.3971012/94‑1 (MQ=60)

                                                                     CGGCCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGGTGTTGCCGCAGTGGAGGGGGGATAATGTCCAATTTAATCGAGA                          >  SRR4302534.345631/1‑94 (MQ=60)

                                                                        CCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAA                       >  SRR4302534.5418833/1‑94 (MQ=60)

                                                                        CCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAA                       <  SRR4302534.380343/94‑1 (MQ=60)

                                                                        CCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAA                       >  SRR4302534.2916347/1‑94 (MQ=60)

                                                                        CCTGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAA                       >  SRR4302534.5445379/1‑94 (MQ=60)

                                                                          TGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAAC                     <  SRR4302534.4055015/94‑1 (MQ=60)

                                                                          TGAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAAC                     >  SRR4302534.4550643/1‑94 (MQ=60)

                                                                           GAATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACA                    <  SRR4302534.2191526/94‑1 (MQ=60)

                                                                            AATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACAC                   >  SRR4302534.2607093/1‑94 (MQ=60)

                                                                            AATATTATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACAC                   >  SRR4302534.4828644/1‑94 (MQ=60)

                                                                                 TATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGG              >  SRR4302534.112196/1‑94 (MQ=60)

                                                                                 TATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGG              <  SRR4302534.4168470/94‑1 (MQ=60)

                                                                                  ATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGT             >  SRR4302534.3691723/1‑94 (MQ=60)

                                                                                  ATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGT             >  SRR4302534.3689407/1‑94 (MQ=60)

                                                                                  ATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGT             <  SRR4302534.2262061/94‑1 (MQ=60)

                                                                                  ATTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGT             <  SRR4302534.4487917/94‑1 (MQ=60)

                                                                                   TTGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTT            <  SRR4302534.1086787/94‑1 (MQ=60)

                                                                                    TGCAGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTA           <  SRR4302534.3726354/94‑1 (MQ=60)

                                                                                      CAGCAAAGGGTCTGACGAATGCAGCTCAGCAGGTTGTTGCCGCAGTGAAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATC         <  SRR4302534.1800746/94‑1 (MQ=60)

                                                                                       AGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCT        <  SRR4302534.4403939/94‑1 (MQ=60)

                                                                                       AGCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCT        >  SRR4302534.3401806/1‑94 (MQ=60)

                                                                                        GCAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTG       <  SRR4302534.4333092/94‑1 (MQ=60)

                                                                                         CAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGC      >  SRR4302534.2810980/1‑94 (MQ=60)

                                                                                         CAAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGC      <  SRR4302534.4173781/94‑1 (MQ=60)

                                                                                          AAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCC     <  SRR4302534.3236222/94‑1 (MQ=60)

                                                                                          AAAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCC     >  SRR4302534.975810/1‑94 (MQ=60)

                                                                                           AAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCA    >  SRR4302534.2435126/1‑94 (MQ=60)

                                                                                           AAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCA    <  SRR4302534.1746755/94‑1 (MQ=60)

                                                                                           AAGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCA    >  SRR4302534.1712113/1‑94 (MQ=60)

                                                                                            AGGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAG   >  SRR4302534.1122588/1‑94 (MQ=60)

                                                                                             GGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGG  <  SRR4302534.2635537/94‑1 (MQ=60)

                                                                                             GGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGG  <  SRR4302534.1817338/94‑1 (MQ=60)

                                                                                             GGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGG  <  SRR4302534.532508/94‑1 (MQ=60)

TAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGG  >  NC_000913/763259‑763445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`