Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,282,811 T→C V1242A (GTA→GCA)  narG → nitrate reductase 1 alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,282,8110TC100.0% 901.3 / NA 257V1242A (GTA→GCA) narGnitrate reductase 1 alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (113/144);  total (113/144)

Only 100 of 267 total aligned reads displayed.

ATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAC  >  NC_000913/1282721‑1282900
                                                                                          |                                                                                         
 tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGcaca                                                                                        <  2:1222528/93‑1 (MQ=255)
 tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGcaca                                                                                        >  1:167468/1‑93 (MQ=255)
  ggCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAg                                                                                       >  1:3755143/1‑93 (MQ=255)
  ggCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAg                                                                                       <  1:1556468/93‑1 (MQ=255)
  ggCACCGTAGGTGCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAg                                                                                       <  1:4768999/93‑1 (MQ=255)
    cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGga                                                                                     <  2:5889938/93‑1 (MQ=255)
     tccGTAGCTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGTCGAAGGCAATGACCAGGCACAGgag                                                                                    <  1:2929698/92‑1 (MQ=255)
     aCCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGgag                                                                                    <  2:1783092/93‑1 (MQ=255)
      ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGgaga                                                                                   <  2:86060/93‑1 (MQ=255)
       cGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGagag                                                                                  >  2:5747753/1‑93 (MQ=255)
       cGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGagag                                                                                  <  2:4553839/93‑1 (MQ=255)
        gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGc                                                                                 <  1:6356542/93‑1 (MQ=255)
        gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGc                                                                                 <  2:1639709/93‑1 (MQ=255)
        gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGc                                                                                 >  2:5039504/1‑93 (MQ=255)
         tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCg                                                                                >  1:5841663/1‑93 (MQ=255)
         tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCg                                                                                <  1:2471537/93‑1 (MQ=255)
          aGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGagag                                                                                  <  1:4102143/90‑1 (MQ=255)
          aGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGt                                                                               >  1:73821/1‑93 (MQ=255)
          aGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGt                                                                               <  2:1987870/93‑1 (MQ=255)
           ggTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTa                                                                              >  1:5114438/1‑93 (MQ=255)
             ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTaaa                                                                            <  2:2185182/93‑1 (MQ=255)
              tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTaaaa                                                                           >  1:90734/1‑93 (MQ=255)
                  aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGaa                                                                       >  1:5919966/1‑93 (MQ=255)
                   ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGaaa                                                                      >  1:3613837/1‑93 (MQ=255)
                   ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGaaa                                                                      >  2:4268559/1‑93 (MQ=255)
                    cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGaaaa                                                                     <  2:1419471/93‑1 (MQ=255)
                     gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAAt                                                                    >  2:249108/1‑93 (MQ=255)
                     gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAAt                                                                    <  1:2220386/93‑1 (MQ=255)
                     gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAAt                                                                    >  2:3067985/1‑93 (MQ=255)
                     gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAAt                                                                    <  2:4154015/93‑1 (MQ=255)
                     gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATAAACACGCACAGAAGAGCGTAAAATGAAAAt                                                                    >  1:4919799/1‑93 (MQ=255)
                      cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAAtt                                                                   <  1:1003691/93‑1 (MQ=255)
                       gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTc                                                                  >  2:6443617/1‑93 (MQ=255)
                       gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTc                                                                  >  2:6329796/1‑93 (MQ=255)
                       gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTc                                                                  <  1:3972674/93‑1 (MQ=255)
                         tGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGt                                                                <  2:2718418/93‑1 (MQ=255)
                         tGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGt                                                                >  1:6055697/1‑93 (MQ=255)
                          gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGtt                                                               <  2:1659970/93‑1 (MQ=255)
                          gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGtt                                                               <  1:6141190/93‑1 (MQ=255)
                            gTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTca                                                             >  2:5093645/1‑93 (MQ=255)
                            gTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTca                                                             >  2:3914394/1‑93 (MQ=255)
                            gTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTca                                                             >  2:1561271/1‑93 (MQ=255)
                            gTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTca                                                             >  2:5525959/1‑93 (MQ=255)
                            gTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTCAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTca                                                             <  2:4457199/93‑1 (MQ=255)
                                gttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAg                                                         <  1:4043877/93‑1 (MQ=255)
                                 ttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGt                                                        <  1:3422482/93‑1 (MQ=255)
                                 ttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGt                                                        <  1:132025/93‑1 (MQ=255)
                                   gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCg                                                      <  1:5840714/93‑1 (MQ=255)
                                     aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCTGc                                                    >  2:5235774/1‑93 (MQ=255)
                                     aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGCAAAATGAAAATTCGTTCACAAGTCGGc                                                    >  2:5384079/1‑93 (MQ=255)
                                         cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATgg                                                >  1:1617401/1‑93 (MQ=255)
                                         cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATgg                                                <  2:5344565/93‑1 (MQ=255)
                                         cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATgg                                                <  2:981397/93‑1 (MQ=255)
                                           tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTg                                              >  2:3127229/1‑93 (MQ=255)
                                           tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTg                                              <  1:432265/93‑1 (MQ=255)
                                            aaGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc                                             >  2:3794837/1‑93 (MQ=255)
                                            aaGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc                                             >  2:1097474/1‑93 (MQ=255)
                                            aaGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc                                             <  1:5617594/93‑1 (MQ=255)
                                            aaGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc                                             <  1:2098504/93‑1 (MQ=255)
                                                tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa                                         <  2:4105383/93‑1 (MQ=255)
                                                 gaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt                                        <  1:4934963/93‑1 (MQ=255)
                                                   agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct                                      >  2:43615/1‑93 (MQ=255)
                                                   agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct                                      <  2:3078068/93‑1 (MQ=255)
                                                    gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtctc                                     >  1:445111/1‑93 (MQ=255)
                                                    gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtctc                                     >  1:5465647/1‑93 (MQ=255)
                                                     aaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg                                    >  1:3733889/1‑93 (MQ=255)
                                                     aaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg                                    <  1:2329072/93‑1 (MQ=255)
                                                      aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa                                   >  2:5470339/1‑93 (MQ=255)
                                                      aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa                                   <  1:266266/93‑1 (MQ=255)
                                                       cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt                                  <  2:1899303/93‑1 (MQ=255)
                                                       cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt                                  >  1:2736814/1‑93 (MQ=255)
                                                        aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa                                 >  1:3087366/1‑93 (MQ=255)
                                                        aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa                                 >  1:4503857/1‑93 (MQ=255)
                                                         ttGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa                                >  2:3382587/1‑93 (MQ=255)
                                                           gACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt                              <  2:5648939/93‑1 (MQ=255)
                                                             cTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGc                            >  2:2612203/1‑93 (MQ=255)
                                                               ggTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt                          <  2:6218789/93‑1 (MQ=255)
                                                               ggTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt                          <  1:1205056/93‑1 (MQ=255)
                                                                gTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATc                         >  2:4760070/1‑93 (MQ=255)
                                                                  tAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCgg                       <  1:140223/93‑1 (MQ=255)
                                                                   aGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGc                      >  1:1029962/1‑93 (MQ=255)
                                                                   aGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGc                      <  2:4812022/93‑1 (MQ=255)
                                                                     aTGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg                    >  2:4948451/1‑93 (MQ=255)
                                                                      tagCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc                   <  2:368285/91‑1 (MQ=255)
                                                                      tGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc                   >  1:5675616/1‑93 (MQ=255)
                                                                         cGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac                >  2:2216948/1‑93 (MQ=255)
                                                                           aaGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCacac              >  1:6313125/1‑93 (MQ=255)
                                                                           aaGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCacac              >  2:3330216/1‑93 (MQ=255)
                                                                             ggCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCt            <  2:3697035/93‑1 (MQ=255)
                                                                               cAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGt          <  2:582434/93‑1 (MQ=255)
                                                                               cAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGt          <  1:1817513/93‑1 (MQ=255)
                                                                                 aTGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc        >  1:6001492/1‑93 (MQ=255)
                                                                                 aTGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc        >  1:2632035/1‑93 (MQ=255)
                                                                                 aTGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGGTc        >  1:1452840/1‑93 (MQ=255)
                                                                                   gACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGACGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAg      >  2:3434260/1‑93 (MQ=255)
                                                                                    aCCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTTCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt     <  1:3317729/93‑1 (MQ=255)
                                                                                    aCCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt     <  1:3368788/93‑1 (MQ=255)
                                                                                     ccAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGtt    <  1:180772/93‑1 (MQ=255)
                                                                                     ccAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGtt    <  1:97389/93‑1 (MQ=255)
                                                                                       aGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc  >  2:2122638/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAC  >  NC_000913/1282721‑1282900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTG  >  NC_000913/1282718‑1282903
                                                                                             |                                                                                            
ACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGC                                                                                              >  SRR4302534.3990633/1‑94 (MQ=60)
ACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGC                                                                                              <  SRR4302534.6633167/94‑1 (MQ=60)
 CTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCA                                                                                             >  SRR4302534.6524551/1‑94 (MQ=60)
   ATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACA                                                                                           <  SRR4302534.2736945/94‑1 (MQ=60)
   ATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACA                                                                                           >  SRR4302534.171868/1‑94 (MQ=60)
   ATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACA                                                                                           >  SRR4302534.2872739/1‑94 (MQ=60)
    TGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTTCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAG                                                                                          <  SRR4302534.4020343/94‑1 (MQ=60)
    TGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAG                                                                                          <  SRR4302534.1605396/94‑1 (MQ=60)
    TGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAG                                                                                          >  SRR4302534.3855977/1‑94 (MQ=60)
     GGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGG                                                                                         <  SRR4302534.1597219/94‑1 (MQ=60)
     GGCACCGTAGGTGCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGG                                                                                         <  SRR4302534.4898478/94‑1 (MQ=60)
      GCTCCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGA                                                                                        <  SRR4302534.4969112/94‑1 (MQ=60)
      GCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGA                                                                                        <  SRR4302534.3330361/94‑1 (MQ=60)
      GCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGA                                                                                        <  SRR4302534.3720230/94‑1 (MQ=60)
       CACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAG                                                                                       <  SRR4302534.1916469/94‑1 (MQ=60)
        TCCGTAGCTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGTCGAAGGCAATGACCAGGCACAGGAGA                                                                                      <  SRR4302534.3006621/94‑1 (MQ=60)
        ACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTGAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGA                                                                                      >  SRR4302534.3569420/1‑94 (MQ=60)
        ACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGA                                                                                      >  SRR4302534.146022/1‑94 (MQ=60)
        ACCGGAGGTTCTAACCGCGATGAGCTTGTTGTAGTGCGTAAGATGCAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGACACAGGAGA                                                                                      >  SRR4302534.6039138/1‑94 (MQ=60)
         CCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAG                                                                                     <  SRR4302534.1974680/94‑1 (MQ=60)
         CCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAG                                                                                     >  SRR4302534.1661041/1‑94 (MQ=60)
          ttTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGC                                                                                    <  SRR4302534.4212449/92‑1 (MQ=60)
          CGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGG                                                                                    >  SRR4302534.1054460/1‑94 (MQ=60)
          CGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGC                                                                                    <  SRR4302534.4230718/94‑1 (MQ=60)
          CGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGC                                                                                    <  SRR4302534.5433853/94‑1 (MQ=60)
           GTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGTGAATGCAATGACCAGGCACAGGAGAGCG                                                                                   >  SRR4302534.3681333/1‑94 (MQ=60)
           GTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCG                                                                                   <  SRR4302534.6528211/94‑1 (MQ=60)
           GTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCG                                                                                   >  SRR4302534.6000240/1‑94 (MQ=60)
            TAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGT                                                                                  >  SRR4302534.75827/1‑94 (MQ=60)
            TAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGT                                                                                  <  SRR4302534.2537226/94‑1 (MQ=60)
             AGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTA                                                                                 >  SRR4302534.5252462/1‑94 (MQ=60)
             AGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTA                                                                                 <  SRR4302534.5062502/94‑1 (MQ=60)
              GGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAA                                                                                >  SRR4302534.1674810/1‑94 (MQ=60)
                TTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAA                                                                              >  SRR4302534.93157/1‑94 (MQ=60)
                 TCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAAT                                                                             <  SRR4302534.2161850/94‑1 (MQ=60)
                 TCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGCAAAAT                                                                             >  SRR4302534.5884995/1‑94 (MQ=60)
                   TAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGA                                                                           >  SRR4302534.942599/1‑94 (MQ=60)
                   TAACCGCGATGAGTTTGTTGCAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGA                                                                           >  SRR4302534.6486409/1‑94 (MQ=60)
                    AACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAA                                                                          >  SRR4302534.6080702/1‑94 (MQ=60)
                    AACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAA                                                                          >  SRR4302534.4699669/1‑94 (MQ=60)
                    AACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAA                                                                          >  SRR4302534.5593875/1‑94 (MQ=60)
                     ACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAA                                                                         >  SRR4302534.3711013/1‑94 (MQ=60)
                       CGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAAT                                                                       >  SRR4302534.2299796/1‑94 (MQ=60)
                       AGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATAAACACGCACAGAAGAGCGTAAAATGAAAAT                                                                       >  SRR4302534.5053139/1‑94 (MQ=60)
                        GCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATT                                                                      >  SRR4302534.712526/1‑94 (MQ=60)
                        GCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATT                                                                      <  SRR4302534.707935/94‑1 (MQ=60)
                        GCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATT                                                                      <  SRR4302534.2279200/94‑1 (MQ=60)
                         CGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTC                                                                     <  SRR4302534.1029440/94‑1 (MQ=60)
                         CGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTC                                                                     >  SRR4302534.1180976/1‑94 (MQ=60)
                          GATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCG                                                                    <  SRR4302534.4079011/94‑1 (MQ=60)
                          GATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCG                                                                    >  SRR4302534.637062/1‑94 (MQ=60)
                           ATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGT                                                                   >  SRR4302534.6220367/1‑94 (MQ=60)
                           ATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGT                                                                   >  SRR4302534.625724/1‑94 (MQ=60)
                            TGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTT                                                                  <  SRR4302534.4530913/94‑1 (MQ=60)
                             GAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTC                                                                 <  SRR4302534.6308225/94‑1 (MQ=60)
                             GAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTC                                                                 <  SRR4302534.273345/94‑1 (MQ=60)
                             GAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTC                                                                 >  SRR4302534.4432745/1‑94 (MQ=60)
                              AGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCA                                                                <  SRR4302534.1860061/94‑1 (MQ=60)
                                 TTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAA                                                             <  SRR4302534.571983/94‑1 (MQ=60)
                                  TGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAG                                                            >  SRR4302534.5094492/1‑94 (MQ=60)
                                   GTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGT                                                           <  SRR4302534.4152323/94‑1 (MQ=60)
                                    TTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTC                                                          <  SRR4302534.135508/94‑1 (MQ=60)
                                    TTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTC                                                          <  SRR4302534.3514443/94‑1 (MQ=60)
                                      GTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGG                                                        <  SRR4302534.5999266/94‑1 (MQ=60)
                                       TAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGC                                                       >  SRR4302534.6238814/1‑94 (MQ=60)
                                       TAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGC                                                       >  SRR4302534.4546557/1‑94 (MQ=60)
                                        AGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGCGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCA                                                      >  SRR4302534.2551237/1‑94 (MQ=60)
                                          TGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATG                                                    <  SRR4302534.1539507/94‑1 (MQ=60)
                                          TGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATG                                                    <  SRR4302534.2595297/94‑1 (MQ=60)
                                          TGCGTAAGAGGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATG                                                    <  SRR4302534.3429779/94‑1 (MQ=60)
                                           GCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGG                                                   >  SRR4302534.1659599/1‑94 (MQ=60)
                                            CGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGT                                                  <  SRR4302534.4882691/94‑1 (MQ=60)
                                             GTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTG                                                 <  SRR4302534.1247364/94‑1 (MQ=60)
                                             ATAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTG                                                 >  SRR4302534.3408773/1‑94 (MQ=60)
                                              TAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGC                                                <  SRR4302534.443289/94‑1 (MQ=60)
                                               AAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCT                                               <  SRR4302534.5769596/94‑1 (MQ=60)
                                               AAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCT                                               <  SRR4302534.2153570/94‑1 (MQ=60)
                                               AAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCT                                               <  SRR4302534.2532504/94‑1 (MQ=60)
                                                    GAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCTTTCACAAGTCGGCATGGTGCTGAATC                                          <  SRR4302534.1395736/94‑1 (MQ=60)
                                                    GAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATC                                          <  SRR4302534.5068699/94‑1 (MQ=60)
                                                    GAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATC                                          <  SRR4302534.1191219/94‑1 (MQ=60)
                                                     AAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCT                                         <  SRR4302534.5184148/94‑1 (MQ=60)
                                                      AGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTC                                        >  SRR4302534.5612975/1‑94 (MQ=60)
                                                      AGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTC                                        >  SRR4302534.456403/1‑94 (MQ=60)
                                                      AGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTC                                        <  SRR4302534.6087238/94‑1 (MQ=60)
                                                       GAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCG                                       >  SRR4302534.3834200/1‑94 (MQ=60)
                                                        AACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGA                                      >  SRR4302534.5775134/1‑94 (MQ=60)
                                                        AACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGA                                      <  SRR4302534.2390872/94‑1 (MQ=60)
                                                         ACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAT                                     <  SRR4302534.3493330/94‑1 (MQ=60)
                                                         ACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAT                                     <  SRR4302534.859771/94‑1 (MQ=60)
                                                         ACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAT                                     <  SRR4302534.5854585/94‑1 (MQ=60)
                                                         ACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAT                                     >  SRR4302534.2808952/1‑94 (MQ=60)
                                                         ACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAT                                     <  SRR4302534.273174/94‑1 (MQ=60)
                                                          CATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATA                                    >  SRR4302534.4625762/1‑94 (MQ=60)
                                                          CATTGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATA                                    >  SRR4302534.3168507/1‑94 (MQ=60)
                                                             TGACTGGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGT                                 >  SRR4302534.3983470/1‑94 (MQ=60)
                                                                  GGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATC                            <  SRR4302534.1236766/94‑1 (MQ=60)
                                                                  GGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATC                            <  SRR4302534.4127603/94‑1 (MQ=60)
                                                                  GGTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATC                            <  SRR4302534.6175159/94‑1 (MQ=60)
                                                                   GTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCG                           <  SRR4302534.2169836/94‑1 (MQ=60)
                                                                   GTTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCG                           <  SRR4302534.3275969/94‑1 (MQ=60)
                                                                    TTAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGG                          <  SRR4302534.1735688/94‑1 (MQ=60)
                                                                     TAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGC                         <  SRR4302534.143896/94‑1 (MQ=60)
                                                                     TAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGC                         >  SRR4302534.610197/1‑94 (MQ=60)
                                                                     TAGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGC                         >  SRR4302534.1056495/1‑94 (MQ=60)
                                                                      AGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCT                        >  SRR4302534.930374/1‑94 (MQ=60)
                                                                      AGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCT                        <  SRR4302534.6065261/94‑1 (MQ=60)
                                                                      AGATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCT                        >  SRR4302534.5809348/1‑94 (MQ=60)
                                                                       GATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTG                       >  SRR4302534.1444883/1‑94 (MQ=60)
                                                                        ATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGC                      >  SRR4302534.4905644/1‑94 (MQ=60)
                                                                        ATGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGC                      >  SRR4302534.5829281/1‑94 (MQ=60)
                                                                         TGGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAACTGCATCGGCTGCC                     >  SRR4302534.3016774/1‑94 (MQ=60)
                                                                          GGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCA                    <  SRR4302534.418170/94‑1 (MQ=60)
                                                                          GGCGAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCA                    <  SRR4302534.4603432/94‑1 (MQ=60)
                                                                             GAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAC                 <  SRR4302534.2876755/94‑1 (MQ=60)
                                                                             GAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAC                 >  SRR4302534.2471825/1‑94 (MQ=60)
                                                                             GAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAC                 >  SRR4302534.131562/1‑94 (MQ=60)
                                                                             GAAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAC                 >  SRR4302534.6483784/1‑94 (MQ=60)
                                                                              AAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACC                >  SRR4302534.3746449/1‑94 (MQ=60)
                                                                              AAGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACC                >  SRR4302534.1506811/1‑94 (MQ=60)
                                                                               AGGCAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCT               >  SRR4302534.5999591/1‑94 (MQ=60)
                                                                                  CAATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTT            <  SRR4302534.1864737/94‑1 (MQ=60)
                                                                                   AATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTC           >  SRR4302534.6164528/1‑94 (MQ=60)
                                                                                   AATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTC           >  SRR4302534.2701677/1‑94 (MQ=60)
                                                                                   AATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTC           <  SRR4302534.4098206/94‑1 (MQ=60)
                                                                                   AATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTC           <  SRR4302534.6390893/94‑1 (MQ=60)
                                                                                   AATGACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGGTC           >  SRR4302534.1491055/1‑94 (MQ=60)
                                                                                      GACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCAGCTACCACACCGGTTCAGT        >  SRR4302534.5907691/1‑94 (MQ=60)
                                                                                       ACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTTCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTT       <  SRR4302534.3406418/94‑1 (MQ=60)
                                                                                       ACCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTT       <  SRR4302534.3459043/94‑1 (MQ=60)
                                                                                        CCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTA      <  SRR4302534.185519/94‑1 (MQ=60)
                                                                                        CCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTA      <  SRR4302534.2731897/94‑1 (MQ=60)
                                                                                        CCAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTA      <  SRR4302534.99993/94‑1 (MQ=60)
                                                                                         CAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAC     >  SRR4302534.1727113/1‑94 (MQ=60)
                                                                                         CAGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAC     <  SRR4302534.3835355/94‑1 (MQ=60)
                                                                                          AGGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACC    >  SRR4302534.6111755/1‑94 (MQ=60)
                                                                                           GGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCT   >  SRR4302534.1730365/1‑94 (MQ=60)
                                                                                           GGCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCT   <  SRR4302534.3306675/94‑1 (MQ=60)
                                                                                            GCACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTG  <  SRR4302534.5429443/94‑1 (MQ=60)
                                                                                             |                                                                                            
ACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTG  >  NC_000913/1282718‑1282903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑