Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,460,324	A→G	D59G (GAC→GGC)	b1398 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,460,324	0	A	G	99.6%	878.9 / NA	257	D59G (GAC→GGC)	b1398	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (122/134); minor base T (0/1); total (122/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

CGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGC  >  NC_000913/1460234‑1460413

cGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGcc                                                                                         <  2:2530879/93‑1 (MQ=255)

cGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGcc                                                                                         >  2:1538357/1‑93 (MQ=255)

cGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGcc                                                                                         >  2:3757426/1‑93 (MQ=255)

 gCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCt                                                                                        >  1:278096/1‑93 (MQ=255)

  cTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTg                                                                                       >  2:2343519/1‑93 (MQ=255)

    cAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCg                                                                                     <  2:3913758/93‑1 (MQ=255)

       acacGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTaa                                                                                  >  1:1428242/1‑93 (MQ=255)

       acacGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTaa                                                                                  >  1:1975518/1‑93 (MQ=255)

       acacGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTaa                                                                                  >  2:3820151/1‑93 (MQ=255)

         acGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAAt                                                                                <  1:6111961/93‑1 (MQ=255)

           gcgTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAAttt                                                                              >  2:5909270/1‑93 (MQ=255)

             gTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTcc                                                                            >  2:5982455/1‑93 (MQ=255)

             gTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTcc                                                                            <  2:753226/93‑1 (MQ=255)

              tATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTccc                                                                           <  2:3076167/93‑1 (MQ=255)

               aTGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCt                                                                          >  2:4678169/1‑93 (MQ=255)

                    aaTGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTacc                                                                     >  1:3256240/1‑93 (MQ=255)

                     aTGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTacca                                                                    >  1:2951153/1‑93 (MQ=255)

                       gtgCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTaccacc                                                                  <  2:6256060/93‑1 (MQ=255)

                        tgCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTAccacca                                                                 <  1:698193/93‑1 (MQ=255)

                         gCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCaa                                                                >  2:6283682/1‑93 (MQ=255)

                           cGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAAc                                                              >  2:5146840/1‑93 (MQ=255)

                             aTGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAg                                                            <  2:4249790/93‑1 (MQ=255)

                              tGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAgg                                                           <  2:3532578/93‑1 (MQ=255)

                                 aTCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                                        <  2:5669943/93‑1 (MQ=255)

                                 aTCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                                        >  1:1483786/1‑93 (MQ=255)

                                  tCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCt                                                       >  2:1172236/1‑93 (MQ=255)

                                   cGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTg                                                      <  1:3374276/93‑1 (MQ=255)

                                     gcgcAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTgcg                                                    <  1:4016678/93‑1 (MQ=255)

                                      cgcAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTgcgc                                                   <  2:2647172/93‑1 (MQ=255)

                                       gcAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGcgcg                                                  <  2:2718526/93‑1 (MQ=255)

                                         aaaTTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGAc                                                >  2:2236667/1‑93 (MQ=255)

                                         aaaTTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGAc                                                >  2:3343930/1‑93 (MQ=255)

                                          aaTTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACa                                               >  1:3430207/1‑93 (MQ=255)

                                          aaTTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACa                                               >  2:5431943/1‑93 (MQ=255)

                                          aaTTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACa                                               >  1:564141/1‑93 (MQ=255)

                                            ttCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAAc                                             <  2:4888666/93‑1 (MQ=255)

                                               gACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                                          <  1:5256681/93‑1 (MQ=255)

                                               gACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                                          >  2:1353262/1‑93 (MQ=255)

                                               gACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                                          <  2:4034397/93‑1 (MQ=255)

                                               gACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                                          >  2:3383657/1‑93 (MQ=255)

                                                aCGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                                         <  2:6340805/93‑1 (MQ=255)

                                                aCGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                                         >  2:2053677/1‑93 (MQ=255)

                                                 cGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATcc                                        <  2:3863231/93‑1 (MQ=255)

                                                 cGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATcc                                        <  1:1727154/93‑1 (MQ=255)

                                                  gcagcaGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATccc                                       <  1:6233946/93‑1 (MQ=255)

                                                   cagcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCt                                      <  1:5383404/93‑1 (MQ=255)

                                                    agcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCtt                                     <  1:2412684/93‑1 (MQ=255)

                                                    agcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCtt                                     >  2:2520012/1‑93 (MQ=255)

                                                     gcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                                    >  1:1009780/1‑93 (MQ=255)

                                                     gcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                                    <  2:127617/93‑1 (MQ=255)

                                                     gcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                                    <  1:3574763/93‑1 (MQ=255)

                                                     gcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                                    <  1:5464804/93‑1 (MQ=255)

                                                     gcagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                                    >  2:1025223/1‑93 (MQ=255)

                                                      cagGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTg                                   <  1:3849968/93‑1 (MQ=255)

                                                       agGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGa                                  >  2:5788852/1‑93 (MQ=255)

                                                         gCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGaca                                >  2:5005151/1‑93 (MQ=255)

                                                         gCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGaca                                >  1:2393805/1‑93 (MQ=255)

                                                           gTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACAcc                              >  1:3156029/1‑93 (MQ=255)

                                                            tACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCt                             >  1:5838602/1‑93 (MQ=255)

                                                             aCATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCtt                            <  1:145835/93‑1 (MQ=255)

                                                              cATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTCCCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCttt                           <  1:592496/93‑1 (MQ=255)

                                                              cATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCttt                           <  1:3750540/93‑1 (MQ=255)

                                                              cATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCttt                           <  1:5594136/93‑1 (MQ=255)

                                                              cATCCTGATGACTTCAGGGAACTTTCAGGCATGCGTAATTTTCCCTGTACCACCAAACTGGATCTGCGCAACAACTATCCCTTTGACACCttt                           <  1:495000/93‑1 (MQ=255)

                                                               aTCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTg                          <  2:2157550/93‑1 (MQ=255)

                                                               aTCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTg                          >  1:3591188/1‑93 (MQ=255)

                                                                tCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGc                         <  2:4606700/93‑1 (MQ=255)

                                                                 ccTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGcc                        <  1:1283825/93‑1 (MQ=255)

                                                                 ccTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGcc                        >  2:192182/1‑93 (MQ=255)

                                                                   tgatgaTTTCAGGGAACTTTCAGTCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGt                      <  2:6025885/93‑1 (MQ=255)

                                                                   tgatgaTTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGt                      >  1:4839656/1‑93 (MQ=255)

                                                                   tgatgaTTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGt                      <  2:4831537/93‑1 (MQ=255)

                                                                   tgatgaTTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGt                      <  2:6112220/93‑1 (MQ=255)

                                                                      tgatTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc                   <  2:2749178/93‑1 (MQ=255)

                                                                      tgatTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc                   <  1:3697146/93‑1 (MQ=255)

                                                                      tgatTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc                   <  1:788358/93‑1 (MQ=255)

                                                                       gatTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCa                  >  2:953646/1‑93 (MQ=255)

                                                                       gatTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCa                  >  2:1703266/1‑93 (MQ=255)

                                                                         tttCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAAt                <  1:5029283/93‑1 (MQ=255)

                                                                         tttCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAAt                <  1:4633711/93‑1 (MQ=255)

                                                                          ttCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATg               <  1:5541297/93‑1 (MQ=255)

                                                                          ttCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATg               >  1:2123259/1‑93 (MQ=255)

                                                                           tCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATgg              >  2:435060/1‑93 (MQ=255)

                                                                           tCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATgg              <  1:1877933/93‑1 (MQ=255)

                                                                             aGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGaa            >  1:2960670/1‑93 (MQ=255)

                                                                             aGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGaa            >  2:534731/1‑93 (MQ=255)

                                                                             aGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGaa            >  2:1816746/1‑93 (MQ=255)

                                                                              gggAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAAc           >  1:5774346/1‑93 (MQ=255)

                                                                              gggAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAAc           >  1:2224253/1‑93 (MQ=255)

                                                                               ggAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACa          >  1:4894981/1‑93 (MQ=255)

                                                                                 accTTCCAGGCCTGCGTAAATTTCCCTGTACCAGCAAACAGGATCTGCGCGACAACGATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAg        <  1:399659/91‑1 (MQ=255)

                                                                                 aaCTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCTACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAg        <  2:3449969/93‑1 (MQ=255)

                                                                                 aaCTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAg        <  2:3276215/93‑1 (MQ=255)

                                                                                 aaCTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAg        >  2:5827087/1‑93 (MQ=255)

                                                                                   cTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAgtg      >  2:5665976/1‑93 (MQ=255)

                                                                                     ttCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAgtggt    <  2:1234440/93‑1 (MQ=255)

                                                                                     ttCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGAAATCTATCCCTTTGACACCTTTGCCGTGCCAATGGAAGAAgtggt    <  1:5106561/93‑1 (MQ=255)

                                                                                      tCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAgtggtg   >  2:805612/1‑93 (MQ=255)

                                                                                       cAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTgc  >  1:4413500/1‑93 (MQ=255)

                                                                                       cAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTgc  <  2:2245433/93‑1 (MQ=255)

CGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGC  >  NC_000913/1460234‑1460413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCA  >  NC_000913/1460231‑1460416

GGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGG                                                                                              >  SRR4302534.1235528/1‑94 (MQ=60)

GGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGG                                                                                              <  SRR4302534.1268888/94‑1 (MQ=60)

GGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGG                                                                                              <  SRR4302534.2396092/94‑1 (MQ=60)

 GACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGC                                                                                             >  SRR4302534.3266200/1‑94 (MQ=60)

 GACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGC                                                                                             <  SRR4302534.4111665/94‑1 (MQ=60)

   CGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCT                                                                                           >  SRR4302534.285292/1‑94 (MQ=60)

   CGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCT                                                                                           <  SRR4302534.6173939/94‑1 (MQ=60)

   CGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCT                                                                                           <  SRR4302534.2026462/94‑1 (MQ=60)

   CGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCT                                                                                           <  SRR4302534.1615393/94‑1 (MQ=60)

    GCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTG                                                                                          >  SRR4302534.3790388/1‑94 (MQ=60)

     CTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGCCCTGC                                                                                         >  SRR4302534.7855/1‑94 (MQ=60)

      TCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCG                                                                                        >  SRR4302534.5251319/1‑94 (MQ=60)

       CAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGT                                                                                       >  SRR4302534.3673906/1‑94 (MQ=60)

        AAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTA                                                                                      >  SRR4302534.3494468/1‑94 (MQ=60)

         AACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAA                                                                                     >  SRR4302534.3392294/1‑94 (MQ=60)

         AACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAA                                                                                     >  SRR4302534.2027240/1‑94 (MQ=60)

         AACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAA                                                                                     >  SRR4302534.1465829/1‑94 (MQ=60)

           CACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAAT                                                                                   >  SRR4302534.2913806/1‑94 (MQ=60)

            ACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATT                                                                                  <  SRR4302534.6278220/94‑1 (MQ=60)

             CGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTT                                                                                 <  SRR4302534.3911310/94‑1 (MQ=60)

             CGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTT                                                                                 >  SRR4302534.4175666/1‑94 (MQ=60)

               CGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCC                                                                               >  SRR4302534.1394259/1‑94 (MQ=60)

               CGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCC                                                                               >  SRR4302534.2309089/1‑94 (MQ=60)

               CGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCC                                                                               <  SRR4302534.5455504/94‑1 (MQ=60)

                GTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCC                                                                              >  SRR4302534.3054731/1‑94 (MQ=60)

                 TATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCT                                                                             <  SRR4302534.634832/94‑1 (MQ=60)

                  ATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTG                                                                            >  SRR4302534.4056628/1‑94 (MQ=60)

                  ATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTG                                                                            <  SRR4302534.275876/94‑1 (MQ=60)

                     AAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTAC                                                                         <  SRR4302534.5518173/94‑1 (MQ=60)

                      AAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACC                                                                        >  SRR4302534.3342961/1‑94 (MQ=60)

                       AATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCA                                                                       >  SRR4302534.3028544/1‑94 (MQ=60)

                           TGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAA                                                                   <  SRR4302534.715286/94‑1 (MQ=60)

                             CCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAAC                                                                 >  SRR4302534.2959017/1‑94 (MQ=60)

                             CCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAAC                                                                 <  SRR4302534.1372634/94‑1 (MQ=60)

                              CGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACA                                                                >  SRR4302534.6465895/1‑94 (MQ=60)

                               GATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCTTGCGTAAATTTCCCTGTACCACCAAACAG                                                               <  SRR4302534.5823576/94‑1 (MQ=60)

                                ATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGG                                                              <  SRR4302534.2265289/94‑1 (MQ=60)

                                  GTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGTT                                                            <  SRR4302534.5122641/94‑1 (MQ=60)

                                   TATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATC                                                           >  SRR4302534.1522822/1‑94 (MQ=60)

                                   TATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATC                                                           <  SRR4302534.5938788/94‑1 (MQ=60)

                                    ATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCT                                                          <  SRR4302534.527021/94‑1 (MQ=60)

                                    ATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCT                                                          <  SRR4302534.601777/94‑1 (MQ=60)

                                     TCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTG                                                         <  SRR4302534.1995407/94‑1 (MQ=60)

                                      CGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGC                                                        <  SRR4302534.3464699/94‑1 (MQ=60)

                                      CGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGC                                                        <  SRR4302534.233756/94‑1 (MQ=60)

                                        GCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGC                                                      <  SRR4302534.4124348/94‑1 (MQ=60)

                                         CGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCG                                                     <  SRR4302534.5722196/94‑1 (MQ=60)

                                          GCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGA                                                    >  SRR4302534.4667006/1‑94 (MQ=60)

                                          GCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGA                                                    >  SRR4302534.3844914/1‑94 (MQ=60)

                                            AAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACA                                                  >  SRR4302534.578012/1‑94 (MQ=60)

                                            AAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACA                                                  >  SRR4302534.3522359/1‑94 (MQ=60)

                                            AAATTCGACCCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTCTACCACCAAACAGGATCTGCGCGACA                                                  >  SRR4302534.3476668/1‑94 (MQ=60)

                                             AATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAA                                                 >  SRR4302534.505978/1‑94 (MQ=60)

                                                TCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTA                                              <  SRR4302534.1877003/94‑1 (MQ=60)

                                                 CGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAT                                             <  SRR4302534.4874621/94‑1 (MQ=60)

                                                  GACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATC                                            <  SRR4302534.5398730/94‑1 (MQ=60)

                                                   ACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCC                                           <  SRR4302534.5077391/94‑1 (MQ=60)

                                                   ACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCC                                           <  SRR4302534.1762950/94‑1 (MQ=60)

                                                   ACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCC                                           <  SRR4302534.3673687/94‑1 (MQ=60)

                                                    CGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCC                                          <  SRR4302534.1772001/94‑1 (MQ=60)

                                                     GCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCT                                         <  SRR4302534.6403164/94‑1 (MQ=60)

                                                      CAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTT                                        >  SRR4302534.3728333/1‑94 (MQ=60)

                                                      CAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTT                                        <  SRR4302534.5528415/94‑1 (MQ=60)

                                                      CAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTT                                        <  SRR4302534.5785125/94‑1 (MQ=60)

                                                       AGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTT                                       <  SRR4302534.647196/94‑1 (MQ=60)

                                                       AGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTT                                       <  SRR4302534.4512323/94‑1 (MQ=60)

                                                       AGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTT                                       >  SRR4302534.1035733/1‑94 (MQ=60)

                                                       AGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTT                                       <  SRR4302534.6047412/94‑1 (MQ=60)

                                                       AGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTT                                       >  SRR4302534.2252169/1‑94 (MQ=60)

                                                       AGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTT                                       <  SRR4302534.2476847/94‑1 (MQ=60)

                                                        GCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTG                                      <  SRR4302534.3670912/94‑1 (MQ=60)

                                                        GCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTG                                      <  SRR4302534.5612113/94‑1 (MQ=60)

                                                         CAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGA                                     <  SRR4302534.5175842/94‑1 (MQ=60)

                                                         CAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGA                                     >  SRR4302534.3927775/1‑94 (MQ=60)

                                                         CAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGA                                     <  SRR4302534.3953347/94‑1 (MQ=60)

                                                         CAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGA                                     <  SRR4302534.151918/94‑1 (MQ=60)

                                                          AGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGAC                                    <  SRR4302534.5640557/94‑1 (MQ=60)

                                                          AGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGAC                                    >  SRR4302534.5598534/1‑94 (MQ=60)

                                                           GGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACA                                   >  SRR4302534.2457413/1‑94 (MQ=60)

                                                             CGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACC                                 >  SRR4302534.3239151/1‑94 (MQ=60)

                                                              GTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCT                                >  SRR4302534.5997103/1‑94 (MQ=60)

                                                               TACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTT                               >  SRR4302534.3260766/1‑94 (MQ=60)

                                                                ACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTT                              <  SRR4302534.149631/94‑1 (MQ=60)

                                                                 CATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTCCCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTG                             <  SRR4302534.606967/94‑1 (MQ=60)

                                                                 CATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTG                             <  SRR4302534.5745390/94‑1 (MQ=60)

                                                                 CATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTG                             >  SRR4302534.3687776/1‑94 (MQ=60)

                                                                 CATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTG                             <  SRR4302534.3851273/94‑1 (MQ=60)

                                                                 CATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTG                             >  SRR4302534.5018106/1‑94 (MQ=60)

                                                                 CATCCTGATGACTTCAGGGAACTTTCAGGCATGCGTAATTTTCCCTGTACCACCAAACTGGATCTGCGCAACAACTATCCCTTTGACACCTTTG                             <  SRR4302534.507353/94‑1 (MQ=60)

                                                                  ATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGC                            >  SRR4302534.410612/1‑94 (MQ=60)

                                                                   TCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCC                           <  SRR4302534.3182048/94‑1 (MQ=60)

                                                                   TCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCC                           <  SRR4302534.5892502/94‑1 (MQ=60)

                                                                    CCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCG                          <  SRR4302534.6165795/94‑1 (MQ=60)

                                                                    CCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCG                          <  SRR4302534.1317631/94‑1 (MQ=60)

                                                                    CCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGAGCGACAACTATCCCTTTGACACCTTTGCCG                          >  SRR4302534.1292582/1‑94 (MQ=60)

                                                                     CTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGT                         >  SRR4302534.4970929/1‑94 (MQ=60)

                                                                      TGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTG                        <  SRR4302534.2459745/94‑1 (MQ=60)

                                                                       GATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGC                       <  SRR4302534.1497718/94‑1 (MQ=60)

                                                                       GATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGC                       >  SRR4302534.900580/1‑94 (MQ=60)

                                                                        ATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCC                      <  SRR4302534.3446145/94‑1 (MQ=60)

                                                                         TGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCA                     <  SRR4302534.808098/94‑1 (MQ=60)

                                                                         TGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCA                     <  SRR4302534.5643161/94‑1 (MQ=60)

                                                                         TGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCA                     <  SRR4302534.3796543/94‑1 (MQ=60)

                                                                          GATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAA                    <  SRR4302534.4110957/94‑1 (MQ=60)

                                                                           ATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAAT                   <  SRR4302534.4806438/94‑1 (MQ=60)

                                                                            TTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATG                  <  SRR4302534.4759459/94‑1 (MQ=60)

                                                                            TTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATG                  >  SRR4302534.2179172/1‑94 (MQ=60)

                                                                            TTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATG                  <  SRR4302534.5165120/94‑1 (MQ=60)

                                                                             TTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGG                 <  SRR4302534.5690875/94‑1 (MQ=60)

                                                                             TTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGG                 <  SRR4302534.2114548/94‑1 (MQ=60)

                                                                              TCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGA                <  SRR4302534.1926837/94‑1 (MQ=60)

                                                                               CAGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAA               >  SRR4302534.3038235/1‑94 (MQ=60)

                                                                                AGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAAC              >  SRR4302534.5930993/1‑94 (MQ=60)

                                                                                AGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAAC              <  SRR4302534.890534/94‑1 (MQ=60)

                                                                                AGGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAAC              >  SRR4302534.2283159/1‑94 (MQ=60)

                                                                                 GGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTTACACCTTTGCCGTGCCAATGGAACA             >  SRR4302534.5153412/1‑94 (MQ=60)

                                                                                 GGGAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACA             >  SRR4302534.5027695/1‑94 (MQ=60)

                                                                                 GGCAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACA             <  SRR4302534.146778/94‑1 (MQ=60)

                                                                                   GAACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAG           >  SRR4302534.3029454/1‑94 (MQ=60)

                                                                                    AACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGT          >  SRR4302534.355044/1‑94 (MQ=60)

                                                                                    AACTTTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGT          <  SRR4302534.573712/94‑1 (MQ=60)

                                                                                        TTCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGAAATCTATCCCTTTGACACCTTTGCCGTGCCAATGGAAGAAGTGGTG      <  SRR4302534.5244369/94‑1 (MQ=60)

                                                                                         TCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGC     <  SRR4302534.2649631/94‑1 (MQ=60)

                                                                                         TCAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGC     >  SRR4302534.4532637/1‑94 (MQ=60)

                                                                                          CAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCG    >  SRR4302534.4501107/1‑94 (MQ=60)

                                                                                          CAGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCG    >  SRR4302534.2904624/1‑94 (MQ=60)

                                                                                           AGGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGC   >  SRR4302534.6004088/1‑94 (MQ=60)

                                                                                            GGCCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCA  <  SRR4302534.907548/94‑1 (MQ=60)

GGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCA  >  NC_000913/1460231‑1460416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`