Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,006	C→T	P861L (CCG→CTG)	fdnG →	formate dehydrogenase‑N, nitrate‑inducible, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,006	0	C	T	100.0%	973.2 / NA	251	P861L (CCG→CTG)	fdnG	formate dehydrogenase‑N, nitrate‑inducible, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (120/131); total (120/131)

Only 100 of 258 total aligned reads displayed.

GGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATG  >  NC_000913/1547916‑1548095

ggATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGc                                                                                         <  2:6308296/93‑1 (MQ=255)

ggATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGc                                                                                         >  2:653661/1‑93 (MQ=255)

ggATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGc                                                                                         >  2:1991811/1‑93 (MQ=255)

 gATGGGACGCCTGTTTGCCATCAGCAAAATGGCGAAAGGTCCGTTCGCAGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCt                                                                                        <  2:772289/93‑1 (MQ=18)

 gATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTCCGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCt                                                                                        <  1:1650271/93‑1 (MQ=255)

     ggACGCCTGTTTGCCATCAACAAAAGGGCGGAAGGTCCGTTCCCGGAACACTGCGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCAt                                                                                    <  1:1887574/93‑1 (MQ=18)

      gACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATc                                                                                   >  1:2206184/1‑93 (MQ=255)

         gCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGACGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGa                                                                                >  2:2809361/1‑93 (MQ=255)

           cTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAAc                                                                              >  1:3577443/1‑93 (MQ=255)

            tGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACg                                                                             >  1:5411129/1‑93 (MQ=255)

            tGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACg                                                                             >  2:6065307/1‑93 (MQ=255)

            tGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACg                                                                             >  1:4956397/1‑93 (MQ=255)

             ttttGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACgt                                                                            <  2:561521/92‑1 (MQ=255)

               ttGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACgtgg                                                                          >  1:2500659/1‑93 (MQ=255)

                tGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACgtggt                                                                         >  2:2757740/1‑93 (MQ=255)

                 gCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACgtggtg                                                                        <  1:4711743/93‑1 (MQ=255)

                  ccATCAACAAAATGGGGAAGGGTCCGTCCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGgtgt                                                                       <  1:1386757/93‑1 (MQ=18)

                   cATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTc                                                                      <  1:2301086/93‑1 (MQ=255)

                   cATCAACAAAAGGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTc                                                                      <  1:2068154/93‑1 (MQ=255)

                    aTCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCt                                                                     >  1:6151426/1‑93 (MQ=255)

                     tCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTa                                                                    <  2:4793136/93‑1 (MQ=255)

                     tCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTa                                                                    >  2:2957138/1‑93 (MQ=255)

                      caacaaAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCtca                                                                   >  2:5524149/1‑91 (MQ=255)

                       aacaaAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAAc                                                                  <  2:4085358/93‑1 (MQ=255)

                          aaaaTGGCGGAAGGTCCGTCCCCGAAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCg                                                               <  1:5068795/93‑1 (MQ=255)

                           aaaTGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCgg                                                              >  1:2402549/1‑93 (MQ=255)

                           aaaTGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCgg                                                              >  2:5355074/1‑93 (MQ=255)

                           aaaTGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCgg                                                              >  1:4039073/1‑93 (MQ=255)

                           aaaTGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCAgg                                                              <  1:2578440/93‑1 (MQ=255)

                             aTGGCGGAAGGTCCTTTCCCGGAACCCTAGGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGgtt                                                            <  2:1854539/93‑1 (MQ=18)

                             aTGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGgtt                                                            <  2:5704228/93‑1 (MQ=255)

                              tGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGgttg                                                           <  2:221428/93‑1 (MQ=255)

                              tGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGgttg                                                           >  2:3425950/1‑93 (MQ=255)

                                gCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGgttgtt                                                         >  1:4545370/1‑93 (MQ=255)

                                gCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGgttgtt                                                         >  2:4575362/1‑93 (MQ=255)

                                     aGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCt                                                    >  1:1677733/1‑93 (MQ=255)

                                      ggTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTg                                                   <  2:1945926/93‑1 (MQ=255)

                                        tCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTa                                                 <  2:176087/93‑1 (MQ=255)

                                        tCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTa                                                 >  1:3716550/1‑93 (MQ=255)

                                         ccGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTAt                                                >  2:3775292/1‑93 (MQ=255)

                                         ccGTTCCCGCAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTAt                                                <  1:2730723/93‑1 (MQ=255)

                                          cGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATg                                               >  2:5393159/1‑93 (MQ=255)

                                            ttCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGaa                                             <  1:855693/93‑1 (MQ=255)

                                              ccgggAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACa                                           <  1:5617627/90‑1 (MQ=255)

                                              cccGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACa                                           >  1:3023248/1‑93 (MQ=255)

                                               cggaaaCACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACaa                                          <  1:3413039/89‑1 (MQ=255)

                                               ccGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACaa                                          >  2:4234560/1‑93 (MQ=255)

                                               ccGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACaa                                          <  2:4726964/93‑1 (MQ=255)

                                                cGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAg                                         >  2:317643/1‑93 (MQ=255)

                                                 ggAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGa                                        >  2:4658128/1‑93 (MQ=255)

                                                 ggAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGa                                        <  1:5572410/93‑1 (MQ=255)

                                                 gaaaCACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGa                                        <  1:791297/91‑1 (MQ=255)

                                                  gAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAc                                       <  1:1742074/93‑1 (MQ=255)

                                                   aaCACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAcg                                      >  2:1034112/1‑93 (MQ=255)

                                                   aaCACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAcg                                      >  1:4835107/1‑93 (MQ=255)

                                                   aaCACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAcg                                      >  1:3801245/1‑93 (MQ=255)

                                                    acacTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAcgc                                     >  1:4596661/1‑93 (MQ=255)

                                                      ccTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACgcgc                                   <  1:281529/92‑1 (MQ=255)

                                                       cTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCt                                  <  2:1629780/93‑1 (MQ=255)

                                                       cTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCt                                  <  2:5870183/93‑1 (MQ=255)

                                                        tACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTg                                 <  2:2813197/93‑1 (MQ=255)

                                                         aCGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGc                                <  2:446656/93‑1 (MQ=255)

                                                          cGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCg                               >  1:2005205/1‑93 (MQ=255)

                                                           gAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCgg                              >  1:6101402/1‑93 (MQ=255)

                                                           gAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCgg                              >  2:3342134/1‑93 (MQ=255)

                                                            aGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGa                             <  2:2526160/93‑1 (MQ=255)

                                                            aGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGa                             >  1:5478718/1‑93 (MQ=255)

                                                            aGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGa                             >  2:6102831/1‑93 (MQ=255)

                                                            aGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGa                             >  2:594543/1‑93 (MQ=255)

                                                             gCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGAt                            >  2:1138265/1‑93 (MQ=255)

                                                              gcGATTGAATCGCCGCTGGGCACTAACCAGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATg                           <  1:5102167/92‑1 (MQ=255)

                                                              ccGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATg                           <  2:1004090/93‑1 (MQ=255)

                                                               cGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATgg                          <  2:3080221/93‑1 (MQ=255)

                                                               cGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATgg                          <  1:1525008/93‑1 (MQ=255)

                                                                 aTTGAAACGCCGCTGGGCGCTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGt                        <  1:464810/93‑1 (MQ=255)

                                                                  ttGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTa                       <  1:2370886/93‑1 (MQ=255)

                                                                  ttGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTa                       <  1:4533193/93‑1 (MQ=255)

                                                                   tGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTaa                      <  2:173045/93‑1 (MQ=255)

                                                                      aaCGCCGCTGGGCACTAACCTGCTGCATCCGAACGCGGTGTCTAACCCGGTTGTTCGACTGTATGAACAAGACGCGCTGCGGATGGGTaaaaa                   >  2:5142475/1‑93 (MQ=255)

                                                                       aCGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTaaaaaa                  >  2:2132935/1‑93 (MQ=255)

                                                                        cgccgcTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAag                 <  2:1439638/93‑1 (MQ=255)

                                                                         gccgcTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAaga                >  2:6365322/1‑93 (MQ=255)

                                                                           cgcTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGc              >  1:6251381/1‑93 (MQ=255)

                                                                           cgcTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGc              >  2:4856779/1‑93 (MQ=255)

                                                                            gcTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCa             <  2:1597523/93‑1 (MQ=255)

                                                                             cTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAg            <  2:1403584/93‑1 (MQ=255)

                                                                             cTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAg            <  1:4280364/93‑1 (MQ=255)

                                                                              tGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGTGTAAAAAAGAGCAGt           <  1:2970109/93‑1 (MQ=255)

                                                                              tGGGCACTAACCTGCTGCATCCGAACGTGGGGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGt           >  1:5167293/1‑93 (MQ=255)

                                                                               gggCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGtt          <  2:3873097/93‑1 (MQ=255)

                                                                               gggCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGtt          >  2:3033409/1‑93 (MQ=255)

                                                                               gggCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGtt          >  2:2712774/1‑93 (MQ=255)

                                                                               gggCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGtt          >  1:1483646/1‑93 (MQ=255)

                                                                                  cACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTccc       <  2:3620818/93‑1 (MQ=255)

                                                                                  cACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTccc       <  1:1119925/93‑1 (MQ=255)

                                                                                  cACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTccc       >  1:2657890/1‑93 (MQ=255)

                                                                                   aCTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCg      >  1:3988279/1‑93 (MQ=255)

                                                                                   aCTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCg      >  2:748368/1‑93 (MQ=255)

                                                                                     tAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTa    <  2:5040723/93‑1 (MQ=255)

                                                                                       aCCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTAtg  >  2:854657/1‑93 (MQ=255)

GGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATG  >  NC_000913/1547916‑1548095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGG  >  NC_000913/1547913‑1548099

AAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCT                                                                                               >  SRR4302534.1722947/1‑94 (MQ=60)

 AGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTG                                                                                              >  SRR4302534.3672078/1‑94 (MQ=60)

 AGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTG                                                                                              >  SRR4302534.4447733/1‑94 (MQ=60)

 AGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTG                                                                                              <  SRR4302534.1545320/94‑1 (MQ=60)

  GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGC                                                                                             >  SRR4302534.5655320/1‑94 (MQ=60)

   GGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCT                                                                                            >  SRR4302534.6188898/1‑94 (MQ=60)

   GGATGGGACGCCTCTTTCCCATCAACAAAATGGCGGAAGGTCCGTCCCCGA‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCT                                                                                            <  SRR4302534.649609/94‑1 (MQ=60)

    GATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTCCGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTG                                                                                           <  SRR4302534.1693292/94‑1 (MQ=60)

      TGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCA                                                                                         >  SRR4302534.5759691/1‑94 (MQ=60)

      TGGGACGCCTGTTTCCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCA                                                                                         <  SRR4302534.5203622/94‑1 (MQ=60)

        GGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATC                                                                                       >  SRR4302534.2264587/1‑94 (MQ=60)

        GGACGCCTGTTTGCCATCAACAAAAGGGCGGAAGGTCCGTTCCCGG‑AACACTGCGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATC                                                                                       <  SRR4302534.1936792/94‑1 (MQ=60)

          ACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCG                                                                                     >  SRR4302534.6281188/1‑94 (MQ=60)

          ACGCCGGTTTCCGATCAGCAAAATGGCGGAAGGTCCGTTCCCGG‑AGCACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCG                                                                                     <  SRR4302534.4005146/94‑1 (MQ=60)

             CCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAAC                                                                                  >  SRR4302534.1888161/1‑94 (MQ=60)

             CCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAAC                                                                                  >  SRR4302534.3673666/1‑94 (MQ=60)

              CTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACG                                                                                 >  SRR4302534.5556705/1‑94 (MQ=60)

              CTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACG                                                                                 >  SRR4302534.5090619/1‑94 (MQ=60)

              CTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACG                                                                                 >  SRR4302534.6201514/1‑94 (MQ=60)

                GTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTG                                                                               >  SRR4302534.1266639/1‑94 (MQ=60)

                 TTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGG                                                                              >  SRR4302534.2567054/1‑94 (MQ=60)

                   TGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTG                                                                            <  SRR4302534.3898163/94‑1 (MQ=60)

                    GCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGT                                                                           <  SRR4302534.4839670/94‑1 (MQ=60)

                     CCATCAACAAAATGGGGAAGGGTCCGTCCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTC                                                                          <  SRR4302534.1423251/94‑1 (MQ=60)

                     CCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTC                                                                          <  SRR4302534.149919/94‑1 (MQ=60)

                      CATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCT                                                                         >  SRR4302534.6318784/1‑94 (MQ=60)

                      CATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCT                                                                         <  SRR4302534.2362161/94‑1 (MQ=60)

                      CATCAACAAAAGGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCT                                                                         <  SRR4302534.2122246/94‑1 (MQ=60)

                       ATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGATGCATCCGAACGTGGTGTCTA                                                                        <  SRR4302534.3061722/94‑1 (MQ=60)

                        TCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAA                                                                       >  SRR4302534.4895491/1‑94 (MQ=60)

                            CAAAATGGCGGAAGGTCCTTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCG                                                                   <  SRR4302534.999689/94‑1 (MQ=60)

                            AAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCG                                                                   <  SRR4302534.1430795/94‑1 (MQ=60)

                             AAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGG                                                                  >  SRR4302534.2466407/1‑94 (MQ=60)

                             AAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGG                                                                  >  SRR4302534.4147375/1‑94 (MQ=60)

                             AAAATGGCGGAAGGTCCGTCCCCGA‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGG                                                                  <  SRR4302534.5205638/94‑1 (MQ=60)

                              AAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCAGGT                                                                 <  SRR4302534.2646733/94‑1 (MQ=60)

                               AATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTT                                                                <  SRR4302534.2901808/94‑1 (MQ=60)

                                ATGGCGGAAGGTCCGTTCCCGG‑AACACTCCGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTG                                                               <  SRR4302534.2861888/94‑1 (MQ=60)

                                ATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTG                                                               >  SRR4302534.244919/1‑94 (MQ=60)

                                  GGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTT                                                             >  SRR4302534.4668598/1‑94 (MQ=60)

                                  GGCGGAAGGCCCGTTCCCGA‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTT                                                             <  SRR4302534.831159/94‑1 (MQ=60)

                                    gggagGGTCCGTTCCCGG‑ACCACTACGAGCCGATTGAAACTCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCG                                                           <  SRR4302534.3515933/89‑1 (MQ=60)

                                    CGGAAGTTCCGTTCCCGA‑AACCCTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCG                                                           <  SRR4302534.3946117/94‑1 (MQ=60)

                                      GAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTC                                                         >  SRR4302534.2707028/1‑94 (MQ=60)

                                       AAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCT                                                        >  SRR4302534.1721383/1‑94 (MQ=60)

                                       AAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCT                                                        >  SRR4302534.4826614/1‑94 (MQ=60)

                                       AAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACACGGTTGTTCGTCT                                                        >  SRR4302534.548246/1‑94 (MQ=60)

                                        AGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTG                                                       <  SRR4302534.4097089/94‑1 (MQ=60)

                                        AGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTG                                                       >  SRR4302534.2260366/1‑94 (MQ=60)

                                         GGTCCGTCCCCGG‑AACACTAGGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGT                                                      <  SRR4302534.2271576/94‑1 (MQ=60)

                                          GTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTA                                                     >  SRR4302534.2744840/1‑94 (MQ=60)

                                          GTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTA                                                     >  SRR4302534.3816475/1‑94 (MQ=60)

                                          GTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTA                                                     >  SRR4302534.2556506/1‑94 (MQ=60)

                                            CCGTTCCCGC‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATG                                                   <  SRR4302534.2802723/94‑1 (MQ=60)

                                            CCGTCCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATG                                                   <  SRR4302534.6399081/94‑1 (MQ=60)

                                            CCGTCCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATG                                                   <  SRR4302534.5582227/94‑1 (MQ=60)

                                               TTCCGGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAAC                                                <  SRR4302534.4660841/94‑1 (MQ=60)

                                               TTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAAC                                                <  SRR4302534.877291/94‑1 (MQ=60)

                                                TCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACA                                               <  SRR4302534.1779534/94‑1 (MQ=60)

                                                TCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACA                                               >  SRR4302534.3102460/1‑94 (MQ=60)

                                                 CCGGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAA                                              <  SRR4302534.5769629/94‑1 (MQ=60)

                                                 CCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAA                                              >  SRR4302534.2011934/1‑94 (MQ=60)

                                                 CCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAA                                              >  SRR4302534.4570709/1‑94 (MQ=60)

                                                   CGGAAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAG                                             <  SRR4302534.3504710/94‑1 (MQ=60)

                                                   CGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGA                                            >  SRR4302534.5309644/1‑94 (MQ=60)

                                                   CGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGGTGTTCGTCTGTATGAACAAGA                                            >  SRR4302534.1651149/1‑94 (MQ=60)

                                                    GG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAC                                           >  SRR4302534.568949/1‑94 (MQ=60)

                                                    GG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAC                                           <  SRR4302534.5722898/94‑1 (MQ=60)

                                                    GA‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGAC                                           <  SRR4302534.811116/94‑1 (MQ=60)

                                                     G‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACG                                          >  SRR4302534.3903307/1‑94 (MQ=60)

                                                     G‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACG                                          <  SRR4302534.2108792/94‑1 (MQ=60)

                                                     G‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACG                                          >  SRR4302534.4966292/1‑94 (MQ=60)

                                                     G‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACG                                          <  SRR4302534.1787295/94‑1 (MQ=60)

                                                       AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGC                                         >  SRR4302534.2113535/1‑94 (MQ=60)

                                                       AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGC                                         >  SRR4302534.1562405/1‑94 (MQ=60)

                                                       AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGC                                         >  SRR4302534.681280/1‑94 (MQ=60)

                                                       AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGC                                         >  SRR4302534.4721411/1‑94 (MQ=60)

                                                          CCTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCT                                      <  SRR4302534.288803/94‑1 (MQ=60)

                                                          ACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCT                                      <  SRR4302534.32984/94‑1 (MQ=60)

                                                           CTACGAGCCGATTGAAACGGCGCTGGGCACTAACCTGCTGCATCCGCACGTGGTGTCTAACCCGGTTGTTTGTCTGTATGAACAAGACGCGCTG                                     >  SRR4302534.2374774/1‑94 (MQ=60)

                                                           CTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTG                                     <  SRR4302534.1719132/94‑1 (MQ=60)

                                                           CTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTG                                     <  SRR4302534.2300436/94‑1 (MQ=60)

                                                           CTACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTG                                     <  SRR4302534.4997066/94‑1 (MQ=60)

                                                             tgGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTACCCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCG                                   <  SRR4302534.5232730/92‑1 (MQ=60)

                                                             ACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCG                                   >  SRR4302534.303466/1‑94 (MQ=60)

                                                             ACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCG                                   >  SRR4302534.2057682/1‑94 (MQ=60)

                                                             ACGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCG                                   >  SRR4302534.2447988/1‑94 (MQ=60)

                                                              CGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGG                                  >  SRR4302534.6267308/1‑94 (MQ=60)

                                                              CGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGG                                  <  SRR4302534.1832189/94‑1 (MQ=60)

                                                              CGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGG                                  <  SRR4302534.4953889/94‑1 (MQ=60)

                                                              CGAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGG                                  >  SRR4302534.3763399/1‑94 (MQ=60)

                                                               GAGCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGA                                 >  SRR4302534.5626455/1‑94 (MQ=60)

                                                                 GCCGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATG                               >  SRR4302534.4685753/1‑94 (MQ=60)

                                                                  GCGATTGAATCGCCGCTGGGCACTAACCAGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGG                              <  SRR4302534.5239879/94‑1 (MQ=60)

                                                                   CGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGG                             >  SRR4302534.516852/1‑94 (MQ=60)

                                                                   CGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGG                             >  SRR4302534.4780441/1‑94 (MQ=60)

                                                                   CGATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGG                             <  SRR4302534.1565047/94‑1 (MQ=60)

                                                                     ATTGAAACGCCGCTGGGCGCTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTA                           <  SRR4302534.476552/94‑1 (MQ=60)

                                                                     ATTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGGACAAGACGCGCTGCGGATGGGTA                           >  SRR4302534.468791/1‑94 (MQ=60)

                                                                      TTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAA                          <  SRR4302534.2433828/94‑1 (MQ=60)

                                                                      TTGAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAA                          <  SRR4302534.4656040/94‑1 (MQ=60)

                                                                        GAAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAA                        >  SRR4302534.1738560/1‑94 (MQ=60)

                                                                         AAACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAA                       >  SRR4302534.6344204/1‑94 (MQ=60)

                                                                          AACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAA                      >  SRR4302534.2683407/1‑94 (MQ=60)

                                                                           ACGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAG                     <  SRR4302534.4498857/94‑1 (MQ=60)

                                                                            CGCCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGA                    >  SRR4302534.2545223/1‑94 (MQ=60)

                                                                              CCGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGC                  >  SRR4302534.6420873/1‑94 (MQ=60)

                                                                               CGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCA                 <  SRR4302534.3686997/94‑1 (MQ=60)

                                                                               CGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCA                 <  SRR4302534.2818109/94‑1 (MQ=60)

                                                                               CGCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCA                 >  SRR4302534.5283565/1‑94 (MQ=60)

                                                                                GCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAG                <  SRR4302534.4108183/94‑1 (MQ=60)

                                                                                GCTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAG                <  SRR4302534.5296826/94‑1 (MQ=60)

                                                                                 CTGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGT               <  SRR4302534.4396219/94‑1 (MQ=60)

                                                                                 CTGGGCACTAACCTGCTGCATCCGAACGTGGGGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGT               >  SRR4302534.5306574/1‑94 (MQ=60)

                                                                                  TGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGTGTAAAAAAGAGCAGTT              <  SRR4302534.3047879/94‑1 (MQ=60)

                                                                                  TGGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTT              >  SRR4302534.1522677/1‑94 (MQ=60)

                                                                                   GGGCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTC             <  SRR4302534.4722846/94‑1 (MQ=60)

                                                                                     GCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCC           <  SRR4302534.4331162/94‑1 (MQ=60)

                                                                                     GCACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCC           >  SRR4302534.2728154/1‑94 (MQ=60)

                                                                                      CACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCG          >  SRR4302534.2622289/1‑94 (MQ=60)

                                                                                      CACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCG          <  SRR4302534.979512/94‑1 (MQ=60)

                                                                                      CACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCG          <  SRR4302534.1149181/94‑1 (MQ=60)

                                                                                      CACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCG          >  SRR4302534.4094984/1‑94 (MQ=60)

                                                                                       ACTAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGT         >  SRR4302534.1674400/1‑94 (MQ=60)

                                                                                         TAACCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTAT       <  SRR4302534.3166017/94‑1 (MQ=60)

                                                                                            CCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTG    <  SRR4302534.6136699/94‑1 (MQ=60)

                                                                                            CCTGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTG    <  SRR4302534.2180692/94‑1 (MQ=60)

                                                                                             CTGCTGTATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGGATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGCTCCCGTATGTGG   >  SRR4302534.1621046/1‑94 (MQ=60)

                                                                                              TGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGG  <  SRR4302534.5767541/94‑1 (MQ=60)

                                                                                              TGCTGCATCCGAACGAGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGCAAAGAAGAGCAGTTCCCGTATGTGGC  >  SRR4302534.2087879/1‑94 (MQ=60)

AAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGG‑AACACTACGAGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGG  >  NC_000913/1547913‑1548099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`