Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,127	T→C	V469A (GTC→GCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,127	0	T	C	100.0%	613.7 / NA	175	V469A (GTC→GCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (70/105); total (70/105)

Only 100 of 182 total aligned reads displayed.

AGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAA  >  NC_000913/1755037‑1755216

agagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGccc                                                                                         <  2:3032701/93‑1 (MQ=255)

agagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGccc                                                                                         >  2:594766/1‑93 (MQ=255)

agagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGccc                                                                                         <  1:428913/93‑1 (MQ=255)

agagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGccc                                                                                         <  2:1976521/93‑1 (MQ=255)

 gagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACCCCGCATCTGTTCACGCCCt                                                                                        <  2:293433/93‑1 (MQ=255)

 gagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCt                                                                                        >  2:5633908/1‑93 (MQ=255)

 gagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCt                                                                                        <  1:2659988/93‑1 (MQ=255)

  agCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTg                                                                                       <  2:6369959/93‑1 (MQ=255)

  agCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTg                                                                                       <  1:6043292/93‑1 (MQ=255)

  agCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTg                                                                                       >  2:3794141/1‑93 (MQ=255)

    cGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTa                                                                                     >  2:5207311/1‑93 (MQ=255)

    cGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTa                                                                                     <  2:2496535/93‑1 (MQ=255)

     ggTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTaa                                                                                    <  2:3281097/93‑1 (MQ=255)

     ggTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTaa                                                                                    <  2:5023920/93‑1 (MQ=255)

      gTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAat                                                                                   <  2:6229931/93‑1 (MQ=255)

      gTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAat                                                                                   <  2:5066076/93‑1 (MQ=255)

       tCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAata                                                                                  <  1:4485912/93‑1 (MQ=255)

        cTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAatat                                                                                 <  2:3951172/93‑1 (MQ=255)

         tGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATAtt                                                                                <  1:3016196/93‑1 (MQ=255)

         tGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATAtt                                                                                <  1:3646417/93‑1 (MQ=255)

           gCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGc                                                                              <  1:4025026/93‑1 (MQ=255)

           gCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGc                                                                              <  1:2155221/93‑1 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             <  2:512483/93‑1 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             >  1:3530812/1‑93 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             <  1:3714340/93‑1 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             <  2:5670419/93‑1 (MQ=255)

             acacaAAGGTGACGTTGTAGTTATGGTTGCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtt                                                                            <  1:3007170/93‑1 (MQ=255)

              cacaTAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTCCTACCCCCGCATCTGTTCACGCCCTGTAATATTGCttt                                                                           <  2:6475937/93‑1 (MQ=255)

              cacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCttt                                                                           >  1:1965592/1‑93 (MQ=255)

               acaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtttt                                                                          <  1:2954487/93‑1 (MQ=255)

               acaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtttt                                                                          <  2:4656342/93‑1 (MQ=255)

                cgaaGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTtg                                                                         >  1:4934768/3‑93 (MQ=255)

                caAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTtg                                                                         >  1:2252804/1‑93 (MQ=255)

                  aaGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTtgtg                                                                       <  1:2012146/93‑1 (MQ=255)

                   aGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGa                                                                      >  2:1745681/1‑93 (MQ=255)

                    ggTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaa                                                                     <  2:3207265/93‑1 (MQ=255)

                    ggTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaa                                                                     >  1:2435957/1‑93 (MQ=255)

                     gTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaat                                                                    <  2:2768869/93‑1 (MQ=255)

                     gTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaat                                                                    >  1:1675816/1‑93 (MQ=255)

                     gTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaat                                                                    <  1:1183063/93‑1 (MQ=255)

                      tGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaatt                                                                   <  2:1947359/93‑1 (MQ=255)

                      tGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaatt                                                                   <  1:655093/93‑1 (MQ=255)

                        aCGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaa                                                                 <  1:5343058/93‑1 (MQ=255)

                        aCGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaa                                                                 <  1:2791695/93‑1 (MQ=255)

                        aCGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaa                                                                 <  1:2897070/93‑1 (MQ=255)

                        aCGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaa                                                                 <  1:4810374/93‑1 (MQ=255)

                         cGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaat                                                                <  1:5147473/93‑1 (MQ=255)

                          gTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaatt                                                               >  1:2405246/1‑93 (MQ=255)

                          gTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaatt                                                               <  2:4894962/93‑1 (MQ=255)

                           ttGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAttt                                                              >  2:6178032/1‑93 (MQ=255)

                           ttGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAttt                                                              >  1:3452158/1‑93 (MQ=255)

                           ttGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAttt                                                              <  2:3372848/93‑1 (MQ=255)

                           ttGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAttt                                                              >  1:5710234/1‑93 (MQ=255)

                           ttGTAGTTATGGTTGCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAttt                                                              <  1:3179085/93‑1 (MQ=255)

                            tGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTg                                                             >  1:5631244/1‑93 (MQ=255)

                            tGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTg                                                             <  2:2807244/93‑1 (MQ=255)

                             gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGt                                                            <  1:4269034/93‑1 (MQ=255)

                             gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGt                                                            >  1:5960355/1‑93 (MQ=255)

                               aGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGtat                                                          <  2:1032378/93‑1 (MQ=255)

                               aGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGtat                                                          <  2:5444272/93‑1 (MQ=255)

                                      gTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAg                                                   <  2:4064049/93‑1 (MQ=255)

                                       tttCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAgc                                                  >  2:4501988/1‑93 (MQ=255)

                                            ggTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCt                                             <  1:5209151/93‑1 (MQ=255)

                                             gTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTg                                            <  1:4176572/93‑1 (MQ=255)

                                              tGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGACCTGa                                           >  2:2389196/1‑93 (MQ=255)

                                               gCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGAt                                          <  1:1008211/93‑1 (MQ=255)

                                               gCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGAt                                          <  2:730220/93‑1 (MQ=255)

                                               gCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGAt                                          <  2:1521085/93‑1 (MQ=255)

                                               gCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGAt                                          <  1:387526/93‑1 (MQ=255)

                                                cACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATg                                         <  1:5501601/93‑1 (MQ=255)

                                                cACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATg                                         <  1:4524368/93‑1 (MQ=255)

                                                  cTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATggg                                       >  2:4456519/1‑93 (MQ=255)

                                                   tGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgc                                      <  1:5576842/93‑1 (MQ=255)

                                                   tGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgc                                      <  2:1123629/93‑1 (MQ=255)

                                                    ggTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcg                                     >  2:359384/1‑93 (MQ=255)

                                                    ggTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcg                                     >  2:3949919/1‑93 (MQ=255)

                                                     gcaCCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGAGCTGATGGgcgc                                    >  1:6230156/3‑93 (MQ=255)

                                                     gTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcgc                                    <  2:4985828/93‑1 (MQ=255)

                                                     gTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcgc                                    <  1:4875212/93‑1 (MQ=255)

                                                       aCCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtt                                  >  1:1723744/1‑93 (MQ=255)

                                                       aCCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtt                                  >  2:4265557/1‑93 (MQ=255)

                                                          gAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttttt                               <  2:5798407/93‑1 (MQ=255)

                                                          gAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttttt                               <  1:5924206/93‑1 (MQ=255)

                                                            gCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttttttt                             <  2:5149758/93‑1 (MQ=255)

                                                             cGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTttta                            >  2:1705685/1‑93 (MQ=255)

                                                              ggCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTtttat                           >  1:2072030/1‑93 (MQ=255)

                                                               gCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTtttatt                          <  1:1325513/93‑1 (MQ=255)

                                                                cACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTtttattt                         >  2:5373460/1‑93 (MQ=255)

                                                                  ctactaACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTaa                       <  1:985775/93‑1 (MQ=255)

                                                                  ctactaACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTaa                       >  2:1357290/1‑93 (MQ=255)

                                                                   tactacCACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAAt                      <  2:112207/93‑1 (MQ=255)

                                                                      taACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGa                   >  2:4343918/1‑93 (MQ=255)

                                                                       aaCACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGAt                  <  1:4553125/93‑1 (MQ=255)

                                                                         cacCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATaa                >  2:1283223/1‑93 (MQ=255)

                                                                           ccGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAcc              <  2:3206002/93‑1 (MQ=255)

                                                                              cATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGa           <  1:3307224/93‑1 (MQ=255)

                                                                               aTCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGaa          <  2:309080/93‑1 (MQ=255)

                                                                                tCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAg         >  2:4778477/1‑93 (MQ=255)

                                                                                tCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAg         >  2:6299674/1‑93 (MQ=255)

                                                                                 cTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGc        >  1:6046378/1‑93 (MQ=255)

AGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAA  >  NC_000913/1755037‑1755216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAAT  >  NC_000913/1755034‑1755220

TGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGC                                                                                               <  SRR4302534.6020319/94‑1 (MQ=60)

  CAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGTTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCC                                                                                             <  SRR4302534.2022224/94‑1 (MQ=60)

   AGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCT                                                                                            <  SRR4302534.221715/94‑1 (MQ=60)

   AGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCT                                                                                            <  SRR4302534.439863/94‑1 (MQ=60)

    GAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTG                                                                                           <  SRR4302534.2730315/94‑1 (MQ=60)

     AGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGT                                                                                          <  SRR4302534.6207574/94‑1 (MQ=60)

          TCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATAT                                                                                     <  SRR4302534.4607289/94‑1 (MQ=60)

            TGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTG                                                                                   <  SRR4302534.3095158/94‑1 (MQ=60)

            TGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTG                                                                                   <  SRR4302534.3744400/94‑1 (MQ=60)

            TGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTG                                                                                   <  SRR4302534.5758635/94‑1 (MQ=60)

             GGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGC                                                                                  >  SRR4302534.4655004/1‑94 (MQ=60)

              GCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCT                                                                                 <  SRR4302534.4132953/94‑1 (MQ=60)

              GCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCT                                                                                 <  SRR4302534.2212115/94‑1 (MQ=60)

              GCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCT                                                                                 >  SRR4302534.3625782/1‑94 (MQ=60)

               CACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTT                                                                                >  SRR4302534.2790945/1‑94 (MQ=60)

               CACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTT                                                                                <  SRR4302534.3814211/94‑1 (MQ=60)

               CACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTT                                                                                >  SRR4302534.5333026/1‑94 (MQ=60)

                ACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTT                                                                               >  SRR4302534.2017048/1‑94 (MQ=60)

                ACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTT                                                                               >  SRR4302534.6180031/1‑94 (MQ=60)

                ACACAAAGGTGACGTTGTAGTTATGGTTGCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTT                                                                               <  SRR4302534.3085842/94‑1 (MQ=60)

                  ACGAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTG                                                                             >  SRR4302534.5068498/1‑94 (MQ=60)

                  ACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTG                                                                             >  SRR4302534.2312521/1‑94 (MQ=60)

                  ACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTG                                                                             >  SRR4302534.4105149/1‑94 (MQ=60)

                  ACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTG                                                                             <  SRR4302534.3031940/94‑1 (MQ=60)

                    AAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTG                                                                           <  SRR4302534.6586661/94‑1 (MQ=60)

                     AAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGA                                                                          <  SRR4302534.2064787/94‑1 (MQ=60)

                      AGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAA                                                                         >  SRR4302534.2500706/1‑94 (MQ=60)

                       GGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAAT                                                                        <  SRR4302534.2492430/94‑1 (MQ=60)

                       GGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAAT                                                                        >  SRR4302534.1719423/1‑94 (MQ=60)

                        GTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATT                                                                       <  SRR4302534.1214156/94‑1 (MQ=60)

                         TGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTA                                                                      >  SRR4302534.494974/1‑94 (MQ=60)

                         TGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTA                                                                      <  SRR4302534.671051/94‑1 (MQ=60)

                          GACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAA                                                                     >  SRR4302534.3307588/1‑94 (MQ=60)

                           ACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAT                                                                    <  SRR4302534.4940961/94‑1 (MQ=60)

                           ACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAT                                                                    <  SRR4302534.2865117/94‑1 (MQ=60)

                           ACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAT                                                                    <  SRR4302534.2973113/94‑1 (MQ=60)

                           ACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAT                                                                    <  SRR4302534.5487112/94‑1 (MQ=60)

                            CGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATT                                                                   <  SRR4302534.5286360/94‑1 (MQ=60)

                            CGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATT                                                                   >  SRR4302534.2469172/1‑94 (MQ=60)

                            CGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATT                                                                   <  SRR4302534.5461686/94‑1 (MQ=60)

                             GTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTT                                                                  >  SRR4302534.5864931/1‑94 (MQ=60)

                             GTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTT                                                                  >  SRR4302534.3544961/1‑94 (MQ=60)

                             GTTGTAGTTATGGTGTCTGGTGCACTGGTGCCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTT                                                                  <  SRR4302534.1776825/94‑1 (MQ=60)

                              TTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTG                                                                 <  SRR4302534.3652043/94‑1 (MQ=60)

                              TTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTG                                                                 >  SRR4302534.5783652/1‑94 (MQ=60)

                              TTGTAGTTATGGTTGCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTG                                                                 <  SRR4302534.3263056/94‑1 (MQ=60)

                               TGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGT                                                                >  SRR4302534.6122186/1‑94 (MQ=60)

                                GTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTA                                                               <  SRR4302534.4384408/94‑1 (MQ=60)

                                  AGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATA                                                             <  SRR4302534.3895201/94‑1 (MQ=60)

                                               GGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTG                                                <  SRR4302534.5349734/94‑1 (MQ=60)

                                                GTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGA                                               <  SRR4302534.4289399/94‑1 (MQ=60)

                                                  GCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATG                                             <  SRR4302534.1034104/94‑1 (MQ=60)

                                                  GCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATG                                             <  SRR4302534.397435/94‑1 (MQ=60)

                                                   CACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGG                                            <  SRR4302534.4646927/94‑1 (MQ=60)

                                                   CACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGG                                            <  SRR4302534.5649979/94‑1 (MQ=60)

                                                   CACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGG                                            <  SRR4302534.6233599/94‑1 (MQ=60)

                                                     CTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGC                                          <  SRR4302534.4432866/94‑1 (MQ=60)

                                                      TGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCG                                         <  SRR4302534.5570065/94‑1 (MQ=60)

                                                      TGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCG                                         <  SRR4302534.5727472/94‑1 (MQ=60)

                                                       GGCACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGAGCTGATGGGCGC                                        >  SRR4302534.6399284/1‑94 (MQ=60)

                                                        GTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCT                                       <  SRR4302534.1267138/94‑1 (MQ=60)

                                                        GTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCT                                       <  SRR4302534.5007436/94‑1 (MQ=60)

                                                         TACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTT                                      >  SRR4302534.1768516/1‑94 (MQ=60)

                                                          ACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTT                                     <  SRR4302534.4347342/94‑1 (MQ=60)

                                                          ACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTT                                     <  SRR4302534.5959342/94‑1 (MQ=60)

                                                           CCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTT                                    <  SRR4302534.6121770/94‑1 (MQ=60)

                                                            CGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTT                                   <  SRR4302534.4180242/94‑1 (MQ=60)

                                                             GAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTT                                  <  SRR4302534.2103642/94‑1 (MQ=60)

                                                             GAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTT                                  <  SRR4302534.6085063/94‑1 (MQ=60)

                                                               GCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTA                                <  SRR4302534.1951461/94‑1 (MQ=60)

                                                                CGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTAT                               >  SRR4302534.2126239/1‑94 (MQ=60)

                                                                  GCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTT                             <  SRR4302534.1360456/94‑1 (MQ=60)

                                                                     GTACTAACACCGCAGCTATTCACGCCCTGTAATATTGCTTGTGTGCATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAAT                          <  SRR4302534.4829703/94‑1 (MQ=60)

                                                                     CTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAAT                          <  SRR4302534.1010962/94‑1 (MQ=60)

                                                                      TACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATC                         >  SRR4302534.1762275/1‑94 (MQ=60)

                                                                       ACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGTATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCG                        <  SRR4302534.3026925/94‑1 (MQ=60)

                                                                        CTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGACCTGATGGGCGCTTTTTTTATTTAATCGA                       >  SRR4302534.3172076/1‑94 (MQ=60)

                                                                         TAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGAT                      <  SRR4302534.3569358/94‑1 (MQ=60)

                                                                          AACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATA                     <  SRR4302534.4676549/94‑1 (MQ=60)

                                                                            CACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAC                   <  SRR4302534.51391/94‑1 (MQ=60)

                                                                            CACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGACCTGATGGGCGCTTTTTTTATTTAATCGATAAC                   >  SRR4302534.165178/1‑94 (MQ=60)

                                                                             CCCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACC                  <  SRR4302534.2884018/94‑1 (MQ=60)

                                                                              CCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCA                 <  SRR4302534.2383486/94‑1 (MQ=60)

                                                                              CCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGACCTGATGGGCGCTTTTTTTATTTAATCGATAACCA                 >  SRR4302534.792905/1‑94 (MQ=60)

                                                                               CGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAG                >  SRR4302534.3070722/1‑94 (MQ=60)

                                                                                GCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGA               >  SRR4302534.4835965/1‑94 (MQ=60)

                                                                                 CATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAA              <  SRR4302534.3395540/94‑1 (MQ=60)

                                                                                  ATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGGCCTGATGGGCGCTTTTTTTATTTTATCGATAAACAGAAG             >  SRR4302534.3441856/1‑94 (MQ=60)

                                                                                  ATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAG             >  SRR4302534.5798601/1‑94 (MQ=60)

                                                                                   TCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGC            >  SRR4302534.6210741/1‑94 (MQ=60)

                                                                                     TGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGGGCTTTTTTTATTTAATCGATAAACAGAAGCAA          >  SRR4302534.4608913/1‑94 (MQ=60)

                                                                                        TCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTAATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAA       >  SRR4302534.2190321/1‑94 (MQ=60)

                                                                                            GCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAA   >  SRR4302534.4804710/1‑94 (MQ=60)

                                                                                             CCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAAT  <  SRR4302534.3190028/94‑1 (MQ=60)

TGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAAT  >  NC_000913/1755034‑1755220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`