Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,560	+C	intergenic (‑129/‑732)	ppiC ← / → rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,558	1	.	C	100.0%	616.2 / NA	174	intergenic (‑127/‑734)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (90/84); total (90/84)

Only 100 of 184 total aligned reads displayed.

AGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑T‑G‑‑C‑TCGCAGG‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957471‑3957645

aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑T‑ccc                                                                                      <  1:4084224/93‑2 (MQ=255)

aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑T‑ccc                                                                                      >  2:650483/1‑92 (MQ=255)

 gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑T‑ccct                                                                                     >  2:5811627/1‑91 (MQ=255)

   aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑T‑ccctca                                                                                   >  2:5770856/1‑89 (MQ=255)

   aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑T‑ccctca                                                                                   >  2:779203/1‑89 (MQ=255)

       tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAggg                                                                                <  1:2009910/93‑1 (MQ=255)

           cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGgaga                                                                            <  2:6042645/93‑1 (MQ=255)

            aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGGAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGaa                                                                           >  2:4746330/1‑93 (MQ=255)

            aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGgagga                                                                           >  2:2445627/1‑91 (MQ=255)

             gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATTGGAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGGAC‑CCTCAAGGGGAGaaa                                                                          >  1:1166106/1‑93 (MQ=255)

              aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGaaaa                                                                         >  2:5020479/1‑93 (MQ=255)

              aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGaaaa                                                                         >  1:6162142/1‑93 (MQ=255)

                gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACt                                                                       >  1:4294278/1‑93 (MQ=255)

                 cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACtt                                                                      <  1:825080/93‑1 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTa                                                                     >  2:2602340/1‑93 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTa                                                                     >  1:48000/1‑93 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAACCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTa                                                                     <  2:262544/93‑1 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCCAGGGGAGGAAACTTAg                                                                    >  1:514303/1‑93 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAg                                                                    >  1:1849484/1‑93 (MQ=255)

                    aCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAgg                                                                   <  2:4110545/93‑1 (MQ=255)

                     cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGGAAACTTAggg                                                                  >  1:5908735/1‑93 (MQ=255)

                     cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAggg                                                                  <  2:5619305/93‑1 (MQ=255)

                      gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGc                                                                 >  2:1198242/1‑93 (MQ=255)

                      gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGc                                                                 >  2:2149950/1‑93 (MQ=255)

                       tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGcc                                                                <  1:3690437/93‑1 (MQ=255)

                        aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCct                                                               >  2:5155373/1‑93 (MQ=255)

                        aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCct                                                               >  2:6248577/1‑93 (MQ=255)

                         aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:4002641/93‑1 (MQ=255)

                         aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:3876923/93‑1 (MQ=255)

                         aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              >  2:1977139/1‑93 (MQ=255)

                         aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:3559789/93‑1 (MQ=255)

                          ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCctct                                                             >  2:4228165/1‑93 (MQ=255)

                            gtcagtcgGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  2:3545410/93‑1 (MQ=255)

                            gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  1:4564369/93‑1 (MQ=255)

                              cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                         <  1:474921/93‑1 (MQ=255)

                              cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                         <  1:2993103/93‑1 (MQ=255)

                               agtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATggg                                                        >  1:378992/1‑93 (MQ=255)

                               agtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATggg                                                        >  1:3033821/1‑93 (MQ=255)

                               agtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATggg                                                        >  1:6052988/1‑93 (MQ=255)

                                gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       <  1:4103869/93‑1 (MQ=255)

                                gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       <  1:579687/93‑1 (MQ=255)

                                gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       >  2:5323247/1‑93 (MQ=255)

                                 tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      <  1:3275527/93‑1 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  1:1319164/1‑93 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGG‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  1:1886101/1‑93 (MQ=255)

                                    gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGG‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:5896571/1‑93 (MQ=255)

                                      gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 >  2:1888014/1‑93 (MQ=255)

                                       aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGATACAAAg                                                <  2:934872/93‑1 (MQ=255)

                                        gTGGCTGGTACATATATGGCAAAAGCGAGGAAAATCC‑G‑GGTT‑TCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                               <  1:3465299/93‑1 (MQ=255)

                                        gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                               <  1:5091477/93‑1 (MQ=255)

                                        gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                               >  2:1478675/1‑93 (MQ=255)

                                         tGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTCCAAAGTc                                              <  2:3252400/93‑1 (MQ=255)

                                         tGGCGGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                              <  1:2343987/93‑1 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  1:341292/93‑1 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  1:4139465/93‑1 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  1:5384875/93‑1 (MQ=255)

                                             tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          <  2:3845239/93‑1 (MQ=255)

                                             tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          >  1:6438646/1‑93 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  1:1013719/93‑1 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  2:2336390/93‑1 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         >  2:3270577/1‑93 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTAGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         >  2:6398462/1‑93 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAATCC‑GGG‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         >  2:5746950/1‑93 (MQ=255)

                                               gTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                        <  1:1477462/93‑1 (MQ=255)

                                               gTACATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                        >  2:568484/1‑93 (MQ=255)

                                                 aCATATATGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      >  2:2995095/1‑93 (MQ=255)

                                                   atatatGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    <  2:3980844/93‑1 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   <  2:3593355/93‑1 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   <  2:5788174/93‑1 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAATCC‑GGG‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  2:3746387/1‑93 (MQ=255)

                                                     atatGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                                  >  2:5184002/1‑93 (MQ=255)

                                                      tatGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                                 <  1:5436679/93‑1 (MQ=255)

                                                       atGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATgg                                >  2:5222339/1‑93 (MQ=255)

                                                       atGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATgg                                <  2:4920529/93‑1 (MQ=255)

                                                        tGGCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                               <  2:2527709/93‑1 (MQ=255)

                                                         ggCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                              >  2:3080812/1‑93 (MQ=255)

                                                          gCAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                             >  2:4028089/1‑93 (MQ=255)

                                                           cAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            >  2:1011353/1‑93 (MQ=255)

                                                           cAAAAGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            <  1:404560/93‑1 (MQ=255)

                                                            aaaaGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  1:764691/1‑93 (MQ=255)

                                                             aaaGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                          >  1:3665878/1‑93 (MQ=255)

                                                               aGCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                        >  1:1613609/1‑93 (MQ=255)

                                                                gCCCGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       >  1:2590033/1‑93 (MQ=255)

                                                                gCCCGGAAAATCC‑GGG‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       >  2:6025981/1‑93 (MQ=255)

                                                                 cccGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      <  1:428894/93‑1 (MQ=255)

                                                                  ccGGAAAATCCGG‑G‑‑CTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  1:4423462/1‑93 (MQ=255)

                                                                                 ggcttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         <  2:3282140/89‑1 (MQ=255)

                                                                                 ggcttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         >  1:1695469/5‑93 (MQ=255)

                                                                                 ggcttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         >  1:3029022/5‑93 (MQ=255)

                                                                                 ggcttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         >  1:741001/5‑93 (MQ=255)

                                                                                    ttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      >  2:4992175/2‑93 (MQ=255)

                                                                                    ttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      <  2:3403801/92‑1 (MQ=255)

                                                                                    ttCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      >  2:2126964/2‑93 (MQ=255)

                                                                                     tCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     >  2:632392/1‑93 (MQ=255)

                                                                                      cGCAGG‑TCCCTCAAGTGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata    <  2:3772301/93‑1 (MQ=255)

                                                                                      cGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata    >  2:1354928/1‑93 (MQ=255)

                                                                                      cGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata    <  2:5352997/93‑1 (MQ=255)

                                                                                       gCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  1:5763749/1‑93 (MQ=255)

                                                                                       gCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  2:1889548/1‑93 (MQ=255)

                                                                                        cAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  <  2:6258594/93‑1 (MQ=255)

AGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑T‑G‑‑C‑TCGCAGG‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957471‑3957645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCAGG‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA  >  NC_000913/3957478‑3957652

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGG                                                                                      <  SRR4302534.2062500/94‑1 (MQ=60)

 CCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGA                                                                                     <  SRR4302534.6190444/94‑1 (MQ=60)

  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAG                                                                                    <  SRR4302534.795019/94‑1 (MQ=60)

    CAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAA                                                                                  >  SRR4302534.5248966/1‑94 (MQ=60)

    CAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAA                                                                                  <  SRR4302534.3996831/94‑1 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAA                                                                                 >  SRR4302534.4512416/1‑94 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGACCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAA                                                                                 >  SRR4302534.3971122/1‑94 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATTGGAAAAGCCCGGAAAATCCGGGCTTCGCAGGAC‑CCTCAAGGGGAGAAA                                                                                 >  SRR4302534.1196732/1‑94 (MQ=55)

      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAA                                                                                >  SRR4302534.6329751/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACT                                                                              >  SRR4302534.3096812/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACT                                                                              >  SRR4302534.4410637/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACT                                                                              <  SRR4302534.3472199/94‑1 (MQ=60)

         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTT                                                                             >  SRR4302534.1681857/1‑94 (MQ=60)

          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTA                                                                            >  SRR4302534.49284/1‑94 (MQ=60)

          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTA                                                                            <  SRR4302534.845841/94‑1 (MQ=60)

           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCCAGGGGAGGAAACTTAG                                                                           >  SRR4302534.527097/1‑94 (MQ=60)

           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAG                                                                           >  SRR4302534.1897601/1‑94 (MQ=60)

            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGG                                                                          >  SRR4302534.3545891/1‑94 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGGAAACTTAGGG                                                                         >  SRR4302534.6069175/1‑94 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGG                                                                         <  SRR4302534.701958/94‑1 (MQ=60)

                TAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCT                                                                      <  SRR4302534.3789664/94‑1 (MQ=60)

                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                    <  SRR4302534.3981015/94‑1 (MQ=60)

                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                    <  SRR4302534.3655563/94‑1 (MQ=60)

                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                    <  SRR4302534.4109805/94‑1 (MQ=60)

                   TTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTA                                                                   >  SRR4302534.3606222/1‑94 (MQ=60)

                   TTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTA                                                                   <  SRR4302534.4778077/94‑1 (MQ=60)

                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGGCTCTAT                                                                  >  SRR4302534.1309765/1‑94 (MQ=60)

                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                 <  SRR4302534.4688113/94‑1 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                               >  SRR4302534.388686/1‑94 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                               <  SRR4302534.3071325/94‑1 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                               >  SRR4302534.3113374/1‑94 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                               >  SRR4302534.6217582/1‑94 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                               <  SRR4302534.486867/94‑1 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                             <  SRR4302534.593891/94‑1 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                             <  SRR4302534.4214230/94‑1 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                             <  SRR4302534.5549730/94‑1 (MQ=60)

                         GTCAGGGAGGGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                             <  SRR4302534.2612832/94‑1 (MQ=60)

                          TCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTA                                                            <  SRR4302534.3362818/94‑1 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                           >  SRR4302534.1935269/1‑94 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                           >  SRR4302534.1353947/1‑94 (MQ=60)

                              GGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAA                                                        >  SRR4302534.5431166/1‑94 (MQ=60)

                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                       <  SRR4302534.863313/94‑1 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCGAGGAAAATCCGGGTTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                     <  SRR4302534.3558456/94‑1 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                     <  SRR4302534.5228904/94‑1 (MQ=60)

                                  TGGCGGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCA                                                    <  SRR4302534.2406193/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                 <  SRR4302534.350022/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                 <  SRR4302534.5529916/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                 >  SRR4302534.6612174/1‑94 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                 <  SRR4302534.1965628/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                 <  SRR4302534.4251174/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                               <  SRR4302534.4202225/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                               <  SRR4302534.1039809/94‑1 (MQ=60)

                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                              <  SRR4302534.1516317/94‑1 (MQ=60)

                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                              <  SRR4302534.1394588/94‑1 (MQ=60)

                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                              >  SRR4302534.5991848/1‑94 (MQ=60)

                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                         <  SRR4302534.4406000/94‑1 (MQ=60)

                                              ATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                                        <  SRR4302534.3119418/94‑1 (MQ=60)

                                              ATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                                        <  SRR4302534.4185181/94‑1 (MQ=60)

                                               TATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                       <  SRR4302534.463282/94‑1 (MQ=60)

                                               TATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                       <  SRR4302534.2707078/94‑1 (MQ=60)

                                               TATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                       <  SRR4302534.5583095/94‑1 (MQ=60)

                                                ATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGT                                      <  SRR4302534.1624482/94‑1 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                  <  SRR4302534.414899/94‑1 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                  >  SRR4302534.783678/1‑94 (MQ=60)

                                                     AAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                                 <  SRR4302534.3819440/94‑1 (MQ=60)

                                                     AAAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                                 >  SRR4302534.3764399/1‑94 (MQ=60)

                                                      AAAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                                <  SRR4302534.4129597/94‑1 (MQ=60)

                                                       AAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                               <  SRR4302534.2078503/94‑1 (MQ=60)

                                                       AAGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                               >  SRR4302534.1655723/1‑94 (MQ=60)

                                                        AGCCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                              >  SRR4302534.2658596/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                            >  SRR4302534.6292833/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                            >  SRR4302534.4542892/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                            <  SRR4302534.439843/94‑1 (MQ=60)

                                                            cggaaaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                          >  SRR4302534.2297069/16‑94 (MQ=60)

                                                            cggaaaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                          >  SRR4302534.2834157/16‑94 (MQ=60)

                                                              gaaaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAA                        <  SRR4302534.1118955/81‑1 (MQ=60)

                                                              gaaaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAA                        >  SRR4302534.184301/14‑94 (MQ=60)

                                                               aaaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT                       >  SRR4302534.391082/13‑94 (MQ=60)

                                                                aaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                      >  SRR4302534.6553541/12‑94 (MQ=60)

                                                                aaatccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                      >  SRR4302534.5753777/12‑94 (MQ=60)

                                                                  atccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                    <  SRR4302534.2227626/85‑1 (MQ=60)

                                                                   tccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAT                   <  SRR4302534.925338/86‑1 (MQ=60)

                                                                   tccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAT                   >  SRR4302534.2259371/9‑94 (MQ=60)

                                                                    ccgggctTCGCAGG‑TGCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                  >  SRR4302534.4025811/8‑94 (MQ=60)

                                                                    ccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                  <  SRR4302534.2483870/87‑1 (MQ=60)

                                                                    ccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                  >  SRR4302534.4888764/8‑94 (MQ=60)

                                                                    ccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                  >  SRR4302534.3514591/8‑94 (MQ=60)

                                                                    ccgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                  <  SRR4302534.4131458/87‑1 (MQ=60)

                                                                     cgggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA                 <  SRR4302534.2821760/88‑1 (MQ=60)

                                                                      gggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                >  SRR4302534.1739537/6‑94 (MQ=60)

                                                                      gggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                >  SRR4302534.759359/6‑94 (MQ=60)

                                                                      gggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                >  SRR4302534.3108436/6‑94 (MQ=60)

                                                                      gggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                <  SRR4302534.846092/89‑1 (MQ=60)

                                                                       ggctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC               <  SRR4302534.5782844/90‑1 (MQ=60)

                                                                         ctTCGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA             <  SRR4302534.1764753/92‑1 (MQ=60)

                                                                            CGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT          >  SRR4302534.5920113/1‑94 (MQ=60)

                                                                            CGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT          <  SRR4302534.6562212/94‑1 (MQ=60)

                                                                            CGCAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT          >  SRR4302534.3703714/1‑94 (MQ=60)

                                                                              CAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG        >  SRR4302534.1427542/1‑94 (MQ=60)

                                                                              CAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG        >  SRR4302534.1205287/1‑94 (MQ=60)

                                                                              CAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG        >  SRR4302534.2996905/1‑94 (MQ=60)

                                                                              CAGG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG        >  SRR4302534.1631521/1‑94 (MQ=60)

                                                                                GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCA      >  SRR4302534.4529828/1‑94 (MQ=60)

                                                                                  gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT     >  SRR4302534.5425337/4‑94 (MQ=60)

                                                                                  gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT     >  SRR4302534.5999695/4‑94 (MQ=60)

                                                                                   tcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT    <  SRR4302534.2372354/92‑1 (MQ=60)

                                                                                     CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA  >  SRR4302534.2240866/1‑94 (MQ=60)

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCAGG‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA  >  NC_000913/3957478‑3957652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`