Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,877	2 bp→CG	coding (586‑587/2022 nt)	rep →	rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,877	0	G	C	99.6%	844.5 / ‑4.9	239	A196R (GCC→CGC)	rep	rep helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (123/115); minor base A (1/0); total (124/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01
*	NC_000913	3,958,878	0	C	G	100.0%	840.7 / NA	238	A196R (GCC→CGC)	rep	rep helicase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (123/115); total (123/115)

Only 100 of 240 total aligned reads displayed.

GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958791‑3958964

||

gTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATg                                                                                   <  2:2853991/93‑1 (MQ=255)

gTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATg                                                                                   >  2:2359456/1‑93 (MQ=255)

gTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATg                                                                                   >  2:2079918/1‑93 (MQ=255)

  tATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaa                                                                                 >  2:4188385/1‑93 (MQ=255)

  tATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaa                                                                                 <  2:340387/93‑1 (MQ=255)

  tATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaa                                                                                 <  1:6434430/93‑1 (MQ=255)

   aTGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaag                                                                                >  2:6222053/1‑93 (MQ=255)

   aTGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaag                                                                                >  1:872650/1‑93 (MQ=255)

   aTGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaag                                                                                >  1:3010102/1‑93 (MQ=255)

    tGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaaga                                                                               >  2:352072/1‑93 (MQ=255)

    tGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaaga                                                                               <  1:3288667/93‑1 (MQ=255)

     gggCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaagaa                                                                              <  1:449168/93‑1 (MQ=255)

        cTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTc                                                                           <  2:1801068/93‑1 (MQ=255)

         tGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTct                                                                          >  1:5308736/1‑92 (MQ=255)

         tGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTcc                                                                          >  2:737/1‑93 (MQ=255)

         tGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTcc                                                                          >  1:3388484/1‑93 (MQ=255)

          gTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCg                                                                         >  2:5823589/1‑93 (MQ=255)

          gTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCg                                                                         <  2:3679518/93‑1 (MQ=255)

              gatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAg                                                                     <  2:4778347/93‑1 (MQ=255)

                  cacaCCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCt                                                                 <  1:1703100/93‑1 (MQ=255)

                  cacaCCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCt                                                                 <  1:153147/93‑1 (MQ=255)

                   ccacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                <  1:757945/92‑1 (MQ=255)

                    cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  1:3500041/1‑93 (MQ=255)

                    cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  1:6012825/1‑93 (MQ=255)

                    cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  2:2587602/1‑93 (MQ=255)

                    cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  2:2916064/1‑93 (MQ=255)

                      ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  2:2198837/93‑1 (MQ=255)

                      ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  2:1220616/93‑1 (MQ=255)

                      ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             >  2:4829502/1‑93 (MQ=255)

                        tGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGa                                                           >  2:3803015/1‑93 (MQ=255)

                        tGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGa                                                           >  1:3512723/1‑93 (MQ=255)

                         gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  1:5509208/1‑93 (MQ=255)

                         gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  2:5172517/1‑93 (MQ=255)

                             gCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaa                                                      >  2:4524542/1‑93 (MQ=255)

                             gCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaa                                                      >  1:6134424/1‑93 (MQ=255)

                                tGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAtt                                                   >  2:4703129/1‑93 (MQ=255)

                                tGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAtt                                                   >  1:391632/1‑93 (MQ=255)

                                 gTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTc                                                  >  1:5385310/1‑93 (MQ=255)

                                   aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  2:4137967/93‑1 (MQ=255)

                                   aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  1:631539/93‑1 (MQ=255)

                                    aCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCt                                               >  2:164105/1‑93 (MQ=255)

                                     cGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTa                                              <  2:4347458/93‑1 (MQ=255)

                                     cGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTa                                              >  1:2794543/1‑93 (MQ=255)

                                      gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             <  1:5850901/93‑1 (MQ=255)

                                      gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             >  2:5219884/1‑93 (MQ=255)

                                      gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             >  2:5255890/1‑93 (MQ=255)

                                       ttCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATc                                            <  1:5751594/93‑1 (MQ=255)

                                       ttCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATc                                            <  2:1326414/93‑1 (MQ=255)

                                        tctcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATct                                           <  1:2518820/93‑1 (MQ=255)

                                        tctcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATct                                           >  1:5281966/1‑93 (MQ=255)

                                        tctcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATct                                           <  1:4007414/93‑1 (MQ=255)

                                         ctcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctg                                          <  1:1361611/93‑1 (MQ=255)

                                          tcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgc                                         >  1:4215311/1‑93 (MQ=255)

                                          tcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgc                                         <  1:2033284/93‑1 (MQ=255)

                                            gACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgctg                                       <  2:4081368/93‑1 (MQ=255)

                                            gACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgctg                                       >  1:3128648/1‑93 (MQ=255)

                                            gACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgctg                                       >  1:6402911/1‑93 (MQ=255)

                                             aCTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtgg                                      <  1:5438053/93‑1 (MQ=255)

                                             aCTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtgg                                      <  1:668822/93‑1 (MQ=255)

                                                tCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtgg                                   <  1:370050/93‑1 (MQ=255)

                                                tCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggcgg                                   >  2:1705427/1‑93 (MQ=255)

                                                  gatgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAt                                 >  2:5931850/1‑93 (MQ=255)

                                                   atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                <  1:1651650/93‑1 (MQ=255)

                                                   atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                <  1:2886606/93‑1 (MQ=255)

                                                    tgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGa                               >  1:2125140/1‑93 (MQ=255)

                                                     gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  1:5184724/1‑93 (MQ=255)

                                                     gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  1:5666677/1‑93 (MQ=255)

                                                       tCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTa                            <  1:1587950/93‑1 (MQ=255)

                                                         tGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGCATc                          >  2:2306145/1‑93 (MQ=255)

                                                          gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         >  2:3081801/1‑93 (MQ=255)

                                                          gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         >  1:629590/1‑93 (MQ=255)

                                                          gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         >  2:6144177/1‑93 (MQ=255)

                                                           aTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        <  2:5680844/93‑1 (MQ=255)

                                                             tttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGa                      >  1:5595683/1‑93 (MQ=255)

                                                              ttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGac                     <  1:5119646/93‑1 (MQ=255)

                                                              ttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGac                     <  1:1019038/93‑1 (MQ=255)

                                                              ttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGac                     >  2:246898/1‑93 (MQ=255)

                                                               tATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGaca                    >  1:739936/1‑93 (MQ=255)

                                                                  tGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacacca                 <  1:2738557/93‑1 (MQ=255)

                                                                    ccGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaac               <  1:2927603/93‑1 (MQ=255)

                                                                    ccGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaac               <  2:3022913/93‑1 (MQ=255)

                                                                      gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  2:3798366/1‑93 (MQ=255)

                                                                      gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             <  2:6377281/93‑1 (MQ=255)

                                                                      gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGGATCAGGacaccaacac             >  1:4423921/1‑93 (MQ=255)

                                                                       aCGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacc            <  1:2569782/93‑1 (MQ=255)

                                                                        cGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacca           >  2:5399579/1‑93 (MQ=255)

                                                                         gTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccag          >  2:2757270/1‑93 (MQ=255)

                                                                           tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        >  2:5597280/1‑93 (MQ=255)

                                                                           tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        <  1:970310/93‑1 (MQ=255)

                                                                           tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        >  2:726450/1‑93 (MQ=255)

                                                                            gctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcca       >  1:1797490/1‑93 (MQ=255)

                                                                             ctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagccag      >  2:4512526/1‑93 (MQ=255)

                                                                              tgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGt     >  1:5778848/1‑93 (MQ=255)

                                                                              tgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGt     <  2:1096573/93‑1 (MQ=255)

                                                                               gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    <  2:4439015/93‑1 (MQ=255)

                                                                                ctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAt   <  1:4548609/93‑1 (MQ=255)

                                                                                ctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAt   >  1:1214258/1‑93 (MQ=255)

                                                                                ctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAt   <  2:1535632/93‑1 (MQ=255)

                                                                                 tgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATg  <  2:5437116/93‑1 (MQ=255)

                                                                                 tgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATg  <  2:5777179/93‑1 (MQ=255)

||

GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958791‑3958964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  >  NC_000913/3958784‑3958966

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                           >  SRR4302534.3508279/1‑94 (MQ=60)

      TGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATG                                                                                     <  SRR4302534.1077422/94‑1 (MQ=60)

      TGTTATGGGCGTTATGATCCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTTATTTTATTGCCGACGTTGCTGCTGCAACGCAATG                                                                                     <  SRR4302534.4835895/94‑1 (MQ=60)

       GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGA                                                                                    >  SRR4302534.165057/1‑94 (MQ=60)

         TATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                  <  SRR4302534.6607873/94‑1 (MQ=60)

         TATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                  >  SRR4302534.3088867/1‑94 (MQ=60)

         TATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                  >  SRR4302534.894703/1‑94 (MQ=60)

          ATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGA                                                                                 <  SRR4302534.4138558/94‑1 (MQ=60)

           TGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAA                                                                                <  SRR4302534.3376351/94‑1 (MQ=60)

            GGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAG                                                                               <  SRR4302534.460546/94‑1 (MQ=60)

            GGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAG                                                                               <  SRR4302534.1690714/94‑1 (MQ=60)

             GGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGT                                                                              <  SRR4302534.808128/94‑1 (MQ=60)

              GCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTC                                                                             <  SRR4302534.6423500/94‑1 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCT                                                                            >  SRR4302534.5451976/1‑94 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            >  SRR4302534.1532523/1‑94 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            >  SRR4302534.3479371/1‑94 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCG                                                                           >  SRR4302534.596662/1‑94 (MQ=60)

                 GTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGC                                                                          >  SRR4302534.2821941/1‑94 (MQ=60)

                  TATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGGAATGAAGAAGTCCGCA                                                                         >  SRR4302534.6607286/1‑94 (MQ=60)

                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAA                                                                        <  SRR4302534.4949464/94‑1 (MQ=60)

                    TGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAG                                                                       >  SRR4302534.2769910/1‑94 (MQ=60)

                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                     <  SRR4302534.1010156/94‑1 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  <  SRR4302534.1747358/94‑1 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302534.3584366/1‑94 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302534.6536778/1‑94 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302534.2305569/1‑94 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  <  SRR4302534.5591936/94‑1 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302534.2972289/1‑94 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  <  SRR4302534.3623657/94‑1 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  <  SRR4302534.157136/94‑1 (MQ=60)

                          CCACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 <  SRR4302534.776746/94‑1 (MQ=60)

                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 >  SRR4302534.3594162/1‑94 (MQ=60)

                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 >  SRR4302534.6176204/1‑94 (MQ=60)

                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 >  SRR4302534.410785/1‑94 (MQ=60)

                           CACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGC                                                                >  SRR4302534.5822875/1‑94 (MQ=60)

                           CACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGC                                                                <  SRR4302534.5329026/94‑1 (MQ=60)

                            ACCGGACAGCATGTAACGTTCTCGACTTCGATGATCTGATTTTATCGCCGACTTTGCTGTTGCAACGCAAAGAAGAAGTCCGCAAGCGCTGGCA                                                               <  SRR4302534.5597235/94‑1 (MQ=60)

                              CTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGA                                                             >  SRR4302534.3607224/1‑94 (MQ=60)

                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                            >  SRR4302534.5657819/1‑94 (MQ=60)

                                GAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAC                                                           >  SRR4302534.773673/1‑94 (MQ=60)

                                  AAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAA                                                         >  SRR4302534.3361431/1‑94 (MQ=60)

                                   AGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAA                                                        >  SRR4302534.6301287/1‑94 (MQ=60)

                                   AGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAA                                                        >  SRR4302534.3085027/1‑94 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      <  SRR4302534.915358/94‑1 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      >  SRR4302534.591017/1‑94 (MQ=60)

                                      CTGTAGCGTTCTCGACGTCGATGATCTGATTTTATTGCCGACGTGGCGGCTGCAACGCAAGGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATT                                                     <  SRR4302534.5949486/94‑1 (MQ=60)

                                      CTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATT                                                     >  SRR4302534.401646/1‑94 (MQ=60)

                                      CTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATT                                                     <  SRR4302534.529512/94‑1 (MQ=60)

                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                    >  SRR4302534.5530363/1‑94 (MQ=60)

                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                    <  SRR4302534.1118840/94‑1 (MQ=60)

                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                   >  SRR4302534.732335/1‑94 (MQ=60)

                                         TAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGC                                                  >  SRR4302534.5752377/1‑94 (MQ=60)

                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                 <  SRR4302534.646863/94‑1 (MQ=60)

                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                 >  SRR4302534.4659141/1‑94 (MQ=60)

                                           ACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTA                                                >  SRR4302534.2868043/1‑94 (MQ=60)

                                           ACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTA                                                <  SRR4302534.6558483/94‑1 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              <  SRR4302534.6009772/94‑1 (MQ=60)

                                              TTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCT                                             >  SRR4302534.5424582/1‑94 (MQ=60)

                                              TTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCT                                             <  SRR4302534.5907558/94‑1 (MQ=60)

                                              TCCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCT                                             <  SRR4302534.5900243/94‑1 (MQ=60)

                                               TCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTG                                            >  SRR4302534.2904252/1‑94 (MQ=60)

                                               TCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTG                                            <  SRR4302534.2585694/94‑1 (MQ=60)

                                               TCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTG                                            <  SRR4302534.4114721/94‑1 (MQ=60)

                                                CTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGC                                           >  SRR4302534.4329000/1‑94 (MQ=60)

                                                CTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGC                                           >  SRR4302534.543174/1‑94 (MQ=60)

                                                CTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGA                                           <  SRR4302534.1397497/94‑1 (MQ=60)

                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                          <  SRR4302534.2086449/94‑1 (MQ=60)

                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                          <  SRR4302534.2165941/94‑1 (MQ=60)

                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                          >  SRR4302534.3263773/1‑94 (MQ=60)

                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                          >  SRR4302534.3580148/1‑94 (MQ=60)

                                                  CGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTG                                         >  SRR4302534.3210896/1‑94 (MQ=60)

                                                  CGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTG                                         <  SRR4302534.4602654/94‑1 (MQ=60)

                                                  CGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTG                                         >  SRR4302534.6575775/1‑94 (MQ=60)

                                                    ACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGT                                       <  SRR4302534.685119/94‑1 (MQ=60)

                                                    ACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGT                                       <  SRR4302534.5584508/94‑1 (MQ=60)

                                                    ACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGT                                       >  SRR4302534.3920620/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      <  SRR4302534.4631764/94‑1 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      <  SRR4302534.6331392/94‑1 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      <  SRR4302534.3461605/94‑1 (MQ=60)

                                                       gcttgcggacttTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGA                                    >  SRR4302534.2060348/13‑94 (MQ=60)

                                                       TCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGA                                    <  SRR4302534.379481/94‑1 (MQ=60)

                                                         GATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                  <  SRR4302534.5522055/94‑1 (MQ=60)

                                                         GATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                  <  SRR4302534.1385228/94‑1 (MQ=60)

                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                 <  SRR4302534.2962361/94‑1 (MQ=60)

                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                 >  SRR4302534.1796809/1‑94 (MQ=60)

                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                 >  SRR4302534.2181103/1‑94 (MQ=60)

                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                 <  SRR4302534.1694699/94‑1 (MQ=60)

                                                          ATGATCTGATTTTATTGCCGACGTTGCTGATGCAACGCAATGAAGAAGTCCGCAAGGGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                 <  SRR4302534.846255/94‑1 (MQ=60)

                                                           TGATCTGATTTTATTGCCGAGGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302534.6476634/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                <  SRR4302534.5269200/94‑1 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302534.5820080/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302534.5324479/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302534.5655946/1‑94 (MQ=60)

                                                            TATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGT                               <  SRR4302534.6131786/94‑1 (MQ=60)

                                                            GATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGT                               <  SRR4302534.5699562/94‑1 (MQ=60)

                                                              TCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAT                             <  SRR4302534.1629490/94‑1 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           >  SRR4302534.662509/1‑94 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           >  SRR4302534.644859/1‑94 (MQ=60)

                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                          <  SRR4302534.4336912/94‑1 (MQ=60)

                                                                   TTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGA                        >  SRR4302534.5746988/1‑94 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      <  SRR4302534.1045275/94‑1 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      >  SRR4302534.758269/1‑94 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      <  SRR4302534.1858316/94‑1 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      <  SRR4302534.5257880/94‑1 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATC‑GGACAC                     >  SRR4302534.2997932/1‑94 (MQ=60)

                                                                       ATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACC                    <  SRR4302534.5539118/94‑1 (MQ=60)

                                                                        TTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCA                   >  SRR4302534.1727909/1‑94 (MQ=60)

                                                                         TGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAA                  <  SRR4302534.2810728/94‑1 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                >  SRR4302534.1563817/1‑94 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                <  SRR4302534.3004471/94‑1 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               <  SRR4302534.5994566/94‑1 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               >  SRR4302534.430769/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGGATCAGGACACCAACAC               >  SRR4302534.4543368/1‑94 (MQ=60)

                                                                            CGACCTTGCTGGTGCAACGCAATGAAGAAGTCCGCAAACGCTGGCAGAACAAAATTCGGCATCTGCTTGGGGGTGAGTAACAGGACACCCACAC               >  SRR4302534.2317229/1‑94 (MQ=25)

                                                                             GACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACC              >  SRR4302534.2184487/1‑94 (MQ=60)

                                                                              ACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCA             <  SRR4302534.6249024/94‑1 (MQ=60)

                                                                              ACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCA             <  SRR4302534.2854913/94‑1 (MQ=60)

                                                                              ACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCA             <  SRR4302534.2637872/94‑1 (MQ=60)

                                                                                GTTGCTGCTGCAAAGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC           >  SRR4302534.35960/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         >  SRR4302534.1844174/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         <  SRR4302534.995032/94‑1 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         >  SRR4302534.696609/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         >  SRR4302534.3194434/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         >  SRR4302534.3941335/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         <  SRR4302534.5482278/94‑1 (MQ=60)

                                                                                   GCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAG        <  SRR4302534.2592229/94‑1 (MQ=60)

                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT       >  SRR4302534.5935589/1‑94 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      <  SRR4302534.2008446/94‑1 (MQ=60)

                                                                                      GCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAT     <  SRR4302534.1576645/94‑1 (MQ=60)

                                                                                      GCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAT     >  SRR4302534.1246217/1‑94 (MQ=60)

                                                                                       CTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG    <  SRR4302534.4671927/94‑1 (MQ=60)

                                                                                         GCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAAGACCAGCCAGTATGAG  >  SRR4302534.2976132/1‑94 (MQ=60)

                                                                                         GCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  <  SRR4302534.4413512/94‑1 (MQ=60)

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  >  NC_000913/3958784‑3958966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`