Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,960,578 2 bp→CG coding (1266‑1267/1485 nt) gppA ← guanosine pentaphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,960,5780GC100.0% 829.9 / NA 237L423V (CTC→GTC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (109/128);  total (109/128)
*NC_0009133,960,5790CG100.0% 822.2 / NA 237R422R (CGG→CGC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (109/128);  total (109/128)

Only 100 of 241 total aligned reads displayed.

GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960491‑3960665
                                                                                       ||                                                                                      
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAcgcgcg                                                                                    >  2:5511047/1‑93 (MQ=255)
  aaTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc                                                                                  <  2:2434060/93‑1 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 <  2:3323352/93‑1 (MQ=255)
     gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               <  2:2369359/93‑1 (MQ=255)
     gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               >  1:1517471/1‑93 (MQ=255)
     gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               >  1:1909332/1‑93 (MQ=255)
       cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             <  2:1631862/93‑1 (MQ=255)
       cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             >  2:39785/1‑93 (MQ=255)
       cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             >  2:6040513/1‑93 (MQ=255)
         tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           >  1:1426597/1‑93 (MQ=255)
           tctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa                                                                         >  2:5481930/1‑93 (MQ=255)
             tGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtt                                                                       <  2:4961976/93‑1 (MQ=255)
              ggCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAttt                                                                      <  1:2829024/93‑1 (MQ=255)
              ggCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAttt                                                                      >  1:5791022/1‑93 (MQ=255)
               gCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGGACGGGAtttt                                                                     >  1:1303772/1‑93 (MQ=255)
                cACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg                                                                    >  2:2270370/1‑93 (MQ=255)
                cACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg                                                                    <  2:1339064/93‑1 (MQ=255)
                 aCGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc                                                                   <  1:1022583/93‑1 (MQ=255)
                     gaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt                                                               >  2:5819052/1‑93 (MQ=255)
                      aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATg                                                              >  1:4402399/1‑93 (MQ=255)
                       tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGc                                                             <  2:1280424/93‑1 (MQ=255)
                       tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGc                                                             >  2:2444331/1‑93 (MQ=255)
                       tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGGACGGCATTTTGCTGATGc                                                             >  1:3424701/1‑93 (MQ=255)
                        cGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa                                                            <  1:1567676/93‑1 (MQ=255)
                         gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAg                                                           <  1:1546117/93‑1 (MQ=255)
                          tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc                                                          <  1:5178693/93‑1 (MQ=255)
                           cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCg                                                         <  1:5124194/93‑1 (MQ=255)
                           cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCg                                                         <  1:2358534/93‑1 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:5613517/93‑1 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        >  2:4335274/1‑93 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        >  1:2468220/1‑93 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:4941064/93‑1 (MQ=255)
                              ggCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATg                                                      <  2:4303480/93‑1 (MQ=255)
                              ggCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTCCGACGCGCGGCGGTACGGCATTTTGCTGTTGCAGCGATg                                                      >  1:1239910/1‑93 (MQ=255)
                               gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  1:6237255/93‑1 (MQ=255)
                               gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  1:475450/93‑1 (MQ=255)
                               gCGACGGCTGGCAAAAATGATGGCAAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  2:5874085/93‑1 (MQ=255)
                                cGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgag                                                    <  2:5106953/93‑1 (MQ=255)
                                 gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgaga                                                   >  2:1015107/1‑93 (MQ=255)
                                 gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgaga                                                   >  2:4567126/1‑93 (MQ=255)
                                  aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag                                                  >  1:3363074/1‑93 (MQ=255)
                                    ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                >  2:6328476/1‑93 (MQ=255)
                                    ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                <  2:5290281/93‑1 (MQ=255)
                                      cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg                                              <  2:4679913/93‑1 (MQ=255)
                                      cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg                                              >  1:3666551/1‑93 (MQ=255)
                                      cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg                                              <  1:3141636/93‑1 (MQ=255)
                                      cTGACAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg                                              >  2:3211638/1‑93 (MQ=255)
                                       tGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa                                             >  1:5816909/1‑93 (MQ=255)
                                        ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc                                            <  1:1222093/93‑1 (MQ=255)
                                        ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc                                            <  1:1848472/93‑1 (MQ=255)
                                        ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc                                            <  1:5327334/93‑1 (MQ=255)
                                         gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTATGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc                                           >  1:4487120/1‑93 (MQ=255)
                                          cAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCg                                          <  2:196519/93‑1 (MQ=255)
                                           aaaaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg                                         >  2:2535838/1‑93 (MQ=255)
                                            aaaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGa                                        >  2:5940186/1‑93 (MQ=255)
                                             aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAt                                       >  1:5014826/1‑93 (MQ=255)
                                              aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt                                      <  1:4203189/93‑1 (MQ=255)
                                              aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt                                      >  2:5235703/1‑93 (MQ=255)
                                              aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt                                      >  1:2764885/1‑93 (MQ=255)
                                              aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt                                      >  1:1971822/1‑93 (MQ=255)
                                                 gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt                                   <  1:756677/93‑1 (MQ=255)
                                                 gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt                                   <  2:6061484/93‑1 (MQ=255)
                                                 gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt                                   <  1:37615/93‑1 (MQ=255)
                                                  atgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc                                  >  1:5733664/1‑93 (MQ=255)
                                                       cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt                             <  2:5052587/93‑1 (MQ=255)
                                                        aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg                            <  1:3476590/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  1:4072124/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  2:2862118/93‑1 (MQ=255)
                                                           cGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGc                         <  2:1410919/93‑1 (MQ=255)
                                                            gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        <  1:4117529/93‑1 (MQ=255)
                                                             tagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg                       >  1:3907292/1‑93 (MQ=255)
                                                             tagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg                       >  1:2109375/1‑93 (MQ=255)
                                                             tagtaAACGGCAGAGTTGTTCTGAGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg                       <  2:4280947/93‑1 (MQ=255)
                                                              agtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      <  2:2704111/93‑1 (MQ=255)
                                                              agtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      >  2:3589677/1‑93 (MQ=255)
                                                              agtaAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      >  1:6131514/1‑93 (MQ=255)
                                                               gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa                     <  2:3913506/93‑1 (MQ=255)
                                                                taAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAg                    <  1:3342135/93‑1 (MQ=255)
                                                                 aaaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGc                   <  1:4658472/93‑1 (MQ=255)
                                                                   aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 >  1:5977000/1‑93 (MQ=255)
                                                                    cGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTc                <  2:3759279/93‑1 (MQ=255)
                                                                     ggCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCg               <  2:3152378/93‑1 (MQ=255)
                                                                      gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              >  1:3690181/1‑93 (MQ=255)
                                                                       cAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAAGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGcc             >  2:3434443/1‑93 (MQ=255)
                                                                        agagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCa            >  2:3943353/1‑93 (MQ=255)
                                                                        agagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCa            <  1:8402/93‑1 (MQ=255)
                                                                        agagTTGTGCTGCGACGCGCGGCGGTACGGCATTGTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCa            <  1:1667739/93‑1 (MQ=255)
                                                                           gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCa         >  1:2505640/1‑93 (MQ=255)
                                                                           gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCa         >  1:5431501/1‑93 (MQ=255)
                                                                           gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCa         >  1:5473166/1‑93 (MQ=255)
                                                                            ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        <  2:4822240/93‑1 (MQ=255)
                                                                            ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        >  1:1456058/1‑93 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACTGCATTTTGCTGATGCAGCGATGACAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      <  1:663282/93‑1 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      >  2:818537/1‑93 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      <  2:1988778/93‑1 (MQ=255)
                                                                                tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 <  1:2167174/80‑1 (MQ=255)
                                                                                tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 >  2:2167174/1‑80 (MQ=255)
                                                                                 cTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttttt   <  1:3092390/93‑1 (MQ=255)
                                                                                 cTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttttt   <  2:4349949/93‑1 (MQ=255)
                                                                                 cTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttttt   <  2:407965/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960491‑3960665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           <  SRR4302534.2447391/94‑1 (MQ=60)
TAGCCTGTAATGTCATCGCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGAC                                                                                           >  SRR4302534.5502972/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302534.1169870/94‑1 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGAAGAGTTGTTCTGCGACGCGCGG                                                                                     <  SRR4302534.1967762/94‑1 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   <  SRR4302534.4814672/94‑1 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   <  SRR4302534.1558481/94‑1 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 <  SRR4302534.4256921/94‑1 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 >  SRR4302534.1959163/1‑94 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 >  SRR4302534.1557337/1‑94 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 <  SRR4302534.1475472/94‑1 (MQ=60)
           GTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTA                                                                                <  SRR4302534.1589956/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302534.131983/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302534.4130215/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACAGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302534.6522572/94‑1 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             >  SRR4302534.1464115/1‑94 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             <  SRR4302534.6405876/94‑1 (MQ=60)
               TCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGC                                                                            >  SRR4302534.5596944/1‑94 (MQ=60)
               TCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGC                                                                            <  SRR4302534.640877/94‑1 (MQ=60)
                 TCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAT                                                                          >  SRR4302534.377343/1‑94 (MQ=60)
                  CTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATT                                                                         <  SRR4302534.5313033/94‑1 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        >  SRR4302534.5948132/1‑94 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        <  SRR4302534.3771133/94‑1 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        >  SRR4302534.1667298/1‑94 (MQ=60)
                    GGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTT                                                                       <  SRR4302534.2903380/94‑1 (MQ=60)
                    GGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGGACGGGATTTT                                                                       >  SRR4302534.1338163/1‑94 (MQ=60)
                     GCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTG                                                                      <  SRR4302534.2715831/94‑1 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGC                                                                     >  SRR4302534.3168672/1‑94 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGC                                                                     <  SRR4302534.2890070/94‑1 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    >  SRR4302534.1759942/1‑94 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    <  SRR4302534.1048912/94‑1 (MQ=60)
                         GAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGA                                                                  >  SRR4302534.904401/1‑94 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 >  SRR4302534.5830424/1‑94 (MQ=60)
                           GATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATG                                                                >  SRR4302534.4521180/1‑94 (MQ=60)
                           GATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATG                                                                >  SRR4302534.208085/1‑94 (MQ=60)
                            ATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGGACGGCATTTTGCTGATGC                                                               >  SRR4302534.3516711/1‑94 (MQ=60)
                             TCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCA                                                              <  SRR4302534.5340058/94‑1 (MQ=60)
                             TCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCA                                                              >  SRR4302534.3550333/1‑94 (MQ=60)
                             TCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCA                                                              <  SRR4302534.702176/94‑1 (MQ=60)
                              CGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAG                                                             <  SRR4302534.1608738/94‑1 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            <  SRR4302534.5965809/94‑1 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            >  SRR4302534.3682956/1‑94 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            >  SRR4302534.1521135/1‑94 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            <  SRR4302534.1586637/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302534.5318217/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          >  SRR4302534.2533830/1‑94 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          <  SRR4302534.5262553/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          <  SRR4302534.2421137/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          >  SRR4302534.2489400/1‑94 (MQ=60)
                                   CGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTCCGACGCGCGGCGGTACGGCATTTTGCTGTTGCAGCGATG                                                        >  SRR4302534.1272591/1‑94 (MQ=60)
                                   CGGCGACGGCTGGCAAAAATGATGGCCAAGCGTAGTAAACGGCAGAGCTGTTCTGCGACGCGCGGCGCAAACGCATTTTGCTGATGCCTCGATG                                                        >  SRR4302534.3089139/1‑94 (MQ=50)
                                     GCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302534.487412/94‑1 (MQ=60)
                                     GCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302534.6406537/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    <  SRR4302534.4951324/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    >  SRR4302534.3453152/1‑94 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302534.3179178/94‑1 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 >  SRR4302534.627235/1‑94 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302534.343741/94‑1 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                >  SRR4302534.4760885/1‑94 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302534.475172/94‑1 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                >  SRR4302534.3765093/1‑94 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302534.5909504/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               >  SRR4302534.873320/1‑94 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               >  SRR4302534.3370814/1‑94 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               <  SRR4302534.3224262/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               >  SRR4302534.5974787/1‑94 (MQ=60)
                                             TGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAC                                              >  SRR4302534.4404690/1‑94 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302534.3530349/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302534.5908465/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302534.1896564/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302534.5471017/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302534.1254277/94‑1 (MQ=60)
                                              CGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTATGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             >  SRR4302534.4608537/1‑94 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            >  SRR4302534.6083051/1‑94 (MQ=60)
                                                 AAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGA                                          >  SRR4302534.1270184/1‑94 (MQ=60)
                                                  AAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAT                                         >  SRR4302534.5150349/1‑94 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        >  SRR4302534.2837680/1‑94 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        >  SRR4302534.2023436/1‑94 (MQ=60)
                                                    AATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTA                                       <  SRR4302534.4316663/94‑1 (MQ=60)
                                                      TGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGT                                     <  SRR4302534.3948433/94‑1 (MQ=60)
                                                       GATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTC                                    <  SRR4302534.38615/94‑1 (MQ=60)
                                                       GATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTC                                    >  SRR4302534.5889037/1‑94 (MQ=60)
                                                       GATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTA                                    <  SRR4302534.775441/94‑1 (MQ=60)
                                                        ATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCT                                   <  SRR4302534.4315790/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302534.794308/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302534.1998525/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302534.1870121/94‑1 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 >  SRR4302534.5581762/1‑94 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 <  SRR4302534.1100608/94‑1 (MQ=60)
                                                           GCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGT                                >  SRR4302534.307812/1‑94 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               <  SRR4302534.5441966/94‑1 (MQ=60)
                                                             CAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTG                              <  SRR4302534.2571297/94‑1 (MQ=60)
                                                             CAGGCGTAGTAAACGGCAGAGTTGTTATGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTG                              >  SRR4302534.5101928/1‑94 (MQ=60)
                                                              AGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGA                             <  SRR4302534.3570036/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302534.4181395/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            >  SRR4302534.1474558/1‑94 (MQ=60)
                                                                 CGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCA                          <  SRR4302534.1406519/94‑1 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         >  SRR4302534.2164814/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         >  SRR4302534.4012082/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         <  SRR4302534.4228322/94‑1 (MQ=60)
                                                                   TAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGT                        <  SRR4302534.3839656/94‑1 (MQ=60)
                                                                   TAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGT                        <  SRR4302534.192985/94‑1 (MQ=60)
                                                                   TAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGT                        >  SRR4302534.6298309/1‑94 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      >  SRR4302534.4205283/1‑94 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTAGTTGAGCAGTAG                      >  SRR4302534.230177/1‑94 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302534.5496867/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302534.3431575/94‑1 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    >  SRR4302534.3240544/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    >  SRR4302534.2184633/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    >  SRR4302534.6152596/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    >  SRR4302534.4040516/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    <  SRR4302534.4784965/94‑1 (MQ=60)
                                                                        AACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGT                   >  SRR4302534.6139332/1‑94 (MQ=60)
                                                                        AACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGT                   >  SRR4302534.1282429/1‑94 (MQ=60)
                                                                        AACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGT                   <  SRR4302534.2039927/94‑1 (MQ=60)
                                                                         ACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTC                  <  SRR4302534.5234454/94‑1 (MQ=60)
                                                                         ACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTC                  <  SRR4302534.4559523/94‑1 (MQ=60)
                                                                         ACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTC                  <  SRR4302534.2379495/94‑1 (MQ=60)
                                                                           GGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGC                >  SRR4302534.3789402/1‑94 (MQ=60)
                                                                            GCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCC               <  SRR4302534.1593078/94‑1 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             >  SRR4302534.3461502/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             >  SRR4302534.2823365/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             <  SRR4302534.8622/94‑1 (MQ=60)
                                                                              AGAGTTGTGCTGCGACGCGCGGCGGTACGGCATTGTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             <  SRR4302534.1711140/94‑1 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302534.5620732/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302534.5577717/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302534.2572174/1‑94 (MQ=60)
                                                                                 GTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAA          >  SRR4302534.1494366/1‑94 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACTGCATTTTGCTGATGCAGCGATGACAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302534.679423/94‑1 (MQ=60)
                                                                                       CTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT    <  SRR4302534.3173722/94‑1 (MQ=60)
                                                                                       CTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT    >  SRR4302534.3512799/1‑94 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302534.5724134/94‑1 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302534.6108606/94‑1 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302534.1261805/94‑1 (MQ=60)
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑