Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,962,227	T→C	Y340C (TAC→TGC)	rhlB ←	putative ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,962,227	0	T	C	99.6%	899.5 / ‑5.7	259	Y340C (TAC→TGC)	rhlB	putative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (132/126); total (133/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 267 total aligned reads displayed.

GCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACC  >  NC_000913/3962137‑3962316

 cAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAAt                                                                                        <  2:4112320/93‑1 (MQ=255)

 cAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAAt                                                                                        <  2:1324419/93‑1 (MQ=255)

  aGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATc                                                                                       <  1:2264802/93‑1 (MQ=255)

  aGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATc                                                                                       >  2:1243605/1‑93 (MQ=255)

   ggCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCt                                                                                      <  1:27200/93‑1 (MQ=255)

   ggCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCt                                                                                      <  1:954015/93‑1 (MQ=255)

   ggCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCt                                                                                      <  2:6220508/93‑1 (MQ=255)

     cAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTc                                                                                    <  1:3001701/93‑1 (MQ=255)

       aaTTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTcac                                                                                  <  2:2222285/93‑1 (MQ=255)

       aaTTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTcac                                                                                  >  1:5831086/1‑93 (MQ=255)

        aTTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTcaca                                                                                 <  2:2827630/93‑1 (MQ=255)

        aTTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTcaca                                                                                 >  2:6456811/1‑93 (MQ=255)

         ttCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAg                                                                                <  1:4925913/93‑1 (MQ=255)

           cAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTc                                                                              >  2:1939937/1‑93 (MQ=255)

            aaTGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCa                                                                             <  1:2558942/93‑1 (MQ=255)

              tGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGGTTGCGCCTGCGTGACCTGTACGACCAATACGGTGAACGCCATCTTCACAGTTATc                                                                           >  2:1879300/1‑93 (MQ=255)

              tGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATc                                                                           <  1:4559430/93‑1 (MQ=255)

               gCATACTCTTCACACGCCAGTCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCg                                                                          >  1:5219673/1‑93 (MQ=255)

               gCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCg                                                                          >  1:172075/1‑93 (MQ=255)

               gCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCg                                                                          >  2:540701/1‑93 (MQ=255)

                 aTACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCggg                                                                        >  2:4337708/1‑93 (MQ=255)

                  tACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGt                                                                       >  1:165422/1‑93 (MQ=255)

                   aCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTa                                                                      >  1:2181201/1‑93 (MQ=255)

                   aCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTa                                                                      >  1:3140766/1‑93 (MQ=255)

                    ctctTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTaa                                                                     >  2:5277266/1‑93 (MQ=255)

                    ctctTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTaa                                                                     >  1:2288828/1‑93 (MQ=255)

                       ttCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATc                                                                  <  2:5164161/93‑1 (MQ=255)

                         cacaCGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGt                                                                >  2:3072563/1‑93 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               >  1:3642925/1‑93 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               >  2:3288677/1‑93 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               >  2:384864/1‑93 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               <  1:2321483/93‑1 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               >  1:1098803/1‑93 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               >  2:4665068/1‑93 (MQ=255)

                          acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTa                                                               <  1:519423/93‑1 (MQ=255)

                           cacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAg                                                              >  2:831235/1‑93 (MQ=255)

                           cacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAg                                                              >  1:4848770/1‑93 (MQ=255)

                              gCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACTTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTa                                                           <  1:1334614/93‑1 (MQ=255)

                              gCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTa                                                           <  1:180054/93‑1 (MQ=255)

                                cAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTaaa                                                         >  1:3268664/1‑93 (MQ=255)

                                cAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTaaa                                                         <  2:1510487/93‑1 (MQ=255)

                                cAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTaaa                                                         <  1:2066178/93‑1 (MQ=255)

                                 aGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAg                                                        >  1:3528478/1‑93 (MQ=255)

                                 aGGCTGATAGAGTGACCGCGTGCGCCTGCGCGACCTGTACGACCAATACGGTGACCGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAg                                                        <  1:4746444/93‑1 (MQ=255)

                                  ggCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGa                                                       >  2:3028974/1‑93 (MQ=255)

                                  ggCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGa                                                       >  1:3315280/1‑93 (MQ=255)

                                   gCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGAc                                                      >  2:5997011/1‑93 (MQ=255)

                                   gCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGAc                                                      >  1:4444168/1‑93 (MQ=255)

                                    cTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACg                                                     <  1:1525621/93‑1 (MQ=255)

                                    cTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACg                                                     >  1:2939976/1‑93 (MQ=255)

                                     tGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGt                                                    <  2:475824/93‑1 (MQ=255)

                                     tGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGt                                                    <  1:5568805/93‑1 (MQ=255)

                                     tGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGt                                                    <  2:5352207/93‑1 (MQ=255)

                                      gATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTg                                                   >  2:6344996/1‑93 (MQ=255)

                                      gATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTg                                                   >  1:3404257/1‑93 (MQ=255)

                                       aTAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGc                                                  <  2:6110852/93‑1 (MQ=255)

                                         agagTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGt                                                <  1:5302311/93‑1 (MQ=255)

                                          gagTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTc                                               >  1:649770/1‑93 (MQ=255)

                                          gagTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTc                                               >  1:5459007/1‑93 (MQ=255)

                                          gagTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTc                                               <  1:5148757/93‑1 (MQ=255)

                                            gTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCAc                                             >  2:4257504/1‑93 (MQ=255)

                                              gACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGAGGTGCGTCACTg                                           >  1:4105026/1‑93 (MQ=255)

                                               aCCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGc                                          <  2:158748/93‑1 (MQ=255)

                                               aCCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGc                                          <  1:3506354/93‑1 (MQ=255)

                                                 cGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCg                                        <  1:84988/93‑1 (MQ=255)

                                                 cGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCg                                        >  1:3251495/1‑93 (MQ=255)

                                                   cTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGa                                      <  1:3868648/93‑1 (MQ=255)

                                                    ttGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGaa                                     <  1:4253851/93‑1 (MQ=255)

                                                     tGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGCCGTGCGTCACTGCCGGAat                                    >  2:1724742/1‑93 (MQ=255)

                                                     tGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAat                                    <  2:1200654/93‑1 (MQ=255)

                                                     tGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAat                                    >  1:2129158/1‑93 (MQ=255)

                                                      gcgcCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAata                                   <  1:6431996/93‑1 (MQ=255)

                                                      gcgcCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAata                                   <  2:1022856/93‑1 (MQ=255)

                                                       cgcCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAatat                                  <  2:1659853/93‑1 (MQ=255)

                                                        gcCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATg                                 <  2:3455836/93‑1 (MQ=255)

                                                          cTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCa                               <  2:5621613/93‑1 (MQ=255)

                                                               cgACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCa                          >  2:2757867/1‑93 (MQ=255)

                                                                 aCCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCAcg                        >  1:6031776/1‑93 (MQ=255)

                                                                   cTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCAcgcg                      >  1:1176948/1‑93 (MQ=255)

                                                                    tGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCAcgcgc                     >  2:164332/1‑93 (MQ=255)

                                                                      tACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCgg                   <  2:50186/93‑1 (MQ=255)

                                                                       aCGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGc                  >  2:3371398/1‑93 (MQ=255)

                                                                       aCGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGc                  <  2:5711707/93‑1 (MQ=255)

                                                                         gACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCaa                >  1:2935423/1‑93 (MQ=255)

                                                                          aCCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAAc               <  1:1789592/93‑1 (MQ=255)

                                                                           ccAATACGGTGAACTCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACg              >  2:6354351/1‑93 (MQ=255)

                                                                           ccAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACg              >  2:1683226/1‑93 (MQ=255)

                                                                           ccAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACg              <  2:5791494/93‑1 (MQ=255)

                                                                           ccAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACg              >  1:3188393/1‑93 (MQ=255)

                                                                            cAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGt             <  2:2506314/93‑1 (MQ=255)

                                                                            cAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGt             <  2:3606610/93‑1 (MQ=255)

                                                                             aaTACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTc            <  1:2487596/93‑1 (MQ=255)

                                                                              aTACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCg           >  2:5904236/1‑93 (MQ=255)

                                                                               tACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCgg          >  2:3815977/1‑93 (MQ=255)

                                                                                aCGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGt         <  2:208521/93‑1 (MQ=255)

                                                                                  ggTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGACTATGCAAACCACGCGCGGCAACGTCGGTgg       >  2:2586116/1‑93 (MQ=255)

                                                                                  ggTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTgg       >  1:1923983/1‑93 (MQ=255)

                                                                                     gAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCaa    >  1:6008995/1‑93 (MQ=255)

                                                                                     gAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCaa    >  2:4489455/1‑93 (MQ=255)

                                                                                       aCGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAAcc  <  1:4884559/93‑1 (MQ=255)

GCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACC  >  NC_000913/3962137‑3962316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAA  >  NC_000913/3962134‑3962320

ATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGC                                                                                               >  SRR4302534.3496883/1‑94 (MQ=60)

ATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGC                                                                                               <  SRR4302534.5945353/94‑1 (MQ=60)

 TAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCA                                                                                              >  SRR4302534.4231510/1‑94 (MQ=60)

  AGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAA                                                                                             >  SRR4302534.1167266/1‑94 (MQ=60)

   GCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAAT                                                                                            <  SRR4302534.887573/94‑1 (MQ=60)

   GCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAAT                                                                                            >  SRR4302534.232869/1‑94 (MQ=60)

   GCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAAT                                                                                            >  SRR4302534.5110617/1‑94 (MQ=60)

    CAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATC                                                                                           >  SRR4302534.1025370/1‑94 (MQ=60)

    CAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATC                                                                                           >  SRR4302534.3209449/1‑94 (MQ=60)

     AGGCAAATTTAATGCATACTCTTCACACGGCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATTCGCTGAACGCAATCT                                                                                          >  SRR4302534.5741615/1‑94 (MQ=60)

     AGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCT                                                                                          >  SRR4302534.3037284/1‑94 (MQ=60)

     AGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCT                                                                                          <  SRR4302534.2324871/94‑1 (MQ=60)

      GGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTT                                                                                         <  SRR4302534.27928/94‑1 (MQ=60)

      GGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTT                                                                                         >  SRR4302534.3355103/1‑94 (MQ=60)

      GGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTT                                                                                         <  SRR4302534.978307/94‑1 (MQ=60)

        CAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCA                                                                                       <  SRR4302534.3080217/94‑1 (MQ=60)

         AAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCAC                                                                                      >  SRR4302534.5989368/1‑94 (MQ=60)

          AATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACA                                                                                     >  SRR4302534.5789653/1‑94 (MQ=60)

          AATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACA                                                                                     <  SRR4302534.3078076/94‑1 (MQ=60)

           ATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAG                                                                                    >  SRR4302534.1318224/1‑94 (MQ=60)

            TTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGT                                                                                   <  SRR4302534.5059427/94‑1 (MQ=60)

             TCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTC                                                                                  <  SRR4302534.2850643/94‑1 (MQ=60)

             TCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTC                                                                                  >  SRR4302534.145384/1‑94 (MQ=60)

               AATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGATCGCAATCTTCACAGTCAT                                                                                >  SRR4302534.6016527/1‑94 (MQ=60)

               AATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCAT                                                                                <  SRR4302534.2626773/94‑1 (MQ=60)

                 TGCATACTCTTCACACGCCAGTCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCG                                                                              >  SRR4302534.5360557/1‑94 (MQ=60)

                 TGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCG                                                                              <  SRR4302534.4683048/94‑1 (MQ=60)

                 TGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCG                                                                              >  SRR4302534.176611/1‑94 (MQ=60)

                 TGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCG                                                                              <  SRR4302534.1792064/94‑1 (MQ=60)

                   CATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGG                                                                            >  SRR4302534.2356072/1‑94 (MQ=60)

                   CATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGG                                                                            <  SRR4302534.1711427/94‑1 (MQ=60)

                    ATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGT                                                                           >  SRR4302534.3402636/1‑94 (MQ=60)

                    ATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGT                                                                           <  SRR4302534.6296662/94‑1 (MQ=60)

                    ATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGT                                                                           >  SRR4302534.169762/1‑94 (MQ=60)

                     TACTCTTCACAGGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCCATCTTCACAGTCATCGGGTA                                                                          >  SRR4302534.4571880/1‑94 (MQ=60)

                     TACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTA                                                                          >  SRR4302534.3223374/1‑94 (MQ=60)

                     TACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTA                                                                          >  SRR4302534.2238856/1‑94 (MQ=60)

                      ACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAA                                                                         >  SRR4302534.2349571/1‑94 (MQ=60)

                      ACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAA                                                                         >  SRR4302534.3576804/1‑94 (MQ=60)

                       aggcaagggcggtatagtagaggagtgggggcaggcaggggggcgccttgaagGACCAATACGGTGAGCGCAATCTTCACAGTCATCGGGTAAA                                                                        <  SRR4302534.4926911/41‑1 (MQ=59)

                       CTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAA                                                                        >  SRR4302534.4357973/1‑94 (MQ=60)

                       CTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAA                                                                        <  SRR4302534.5659807/94‑1 (MQ=60)

                          TTCACCCGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCG                                                                     <  SRR4302534.2274142/94‑1 (MQ=60)

                          TTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCG                                                                     <  SRR4302534.6185320/94‑1 (MQ=60)

                          TTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCG                                                                     >  SRR4302534.2118070/1‑94 (MQ=60)

                            CACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTA                                                                   >  SRR4302534.3740828/1‑94 (MQ=60)

                            CACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTA                                                                   >  SRR4302534.1127422/1‑94 (MQ=60)

                             ACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAG                                                                  <  SRR4302534.155712/94‑1 (MQ=60)

                             ACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAG                                                                  <  SRR4302534.532339/94‑1 (MQ=60)

                             ACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAG                                                                  >  SRR4302534.4980309/1‑94 (MQ=60)

                             ACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAG                                                                  <  SRR4302534.2383097/94‑1 (MQ=60)

                             ACACCCCAGGCTGATAGAGCGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAG                                                                  >  SRR4302534.736625/1‑94 (MQ=60)

                               ACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTT                                                                <  SRR4302534.3607940/94‑1 (MQ=60)

                                CGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTA                                                               <  SRR4302534.1301948/94‑1 (MQ=60)

                                 GCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACTTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAA                                                              <  SRR4302534.1369788/94‑1 (MQ=60)

                                 GCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAA                                                              <  SRR4302534.184792/94‑1 (MQ=60)

                                  ACAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAA                                                             >  SRR4302534.3355726/1‑94 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            >  SRR4302534.247570/1‑94 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            <  SRR4302534.2120210/94‑1 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            >  SRR4302534.3299566/1‑94 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            <  SRR4302534.5425156/94‑1 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            >  SRR4302534.494219/1‑94 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            >  SRR4302534.3623386/1‑94 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            >  SRR4302534.5038216/1‑94 (MQ=60)

                                   CAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAG                                                            <  SRR4302534.2241307/94‑1 (MQ=60)

                                    AGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTGAAGA                                                           >  SRR4302534.3489266/1‑94 (MQ=60)

                                    AGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGA                                                           <  SRR4302534.5558825/94‑1 (MQ=60)

                                    AGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGA                                                           >  SRR4302534.3403876/1‑94 (MQ=60)

                                    AGGCTGATAGAGTGACCGCGTGCGCCTGCGCGACCTGTACGACCAATACGGTGACCGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGA                                                           <  SRR4302534.4875296/94‑1 (MQ=60)

                                     GGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGAC                                                          <  SRR4302534.2228501/94‑1 (MQ=60)

                                     GGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGAC                                                          >  SRR4302534.4564236/1‑94 (MQ=60)

                                      GCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACG                                                         <  SRR4302534.2754273/94‑1 (MQ=60)

                                      GCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACG                                                         >  SRR4302534.3017128/1‑94 (MQ=60)

                                       CTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGT                                                        <  SRR4302534.5021412/94‑1 (MQ=60)

                                       CTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGT                                                        >  SRR4302534.535007/1‑94 (MQ=60)

                                       CTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGT                                                        <  SRR4302534.1565675/94‑1 (MQ=60)

                                        TGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTG                                                       <  SRR4302534.5719188/94‑1 (MQ=60)

                                        TGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTG                                                       >  SRR4302534.5640679/1‑94 (MQ=60)

                                        TGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTG                                                       >  SRR4302534.3495677/1‑94 (MQ=60)

                                         GATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGC                                                      <  SRR4302534.714723/94‑1 (MQ=60)

                                            AGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTC                                                   >  SRR4302534.5606124/1‑94 (MQ=60)

                                            AGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTC                                                   >  SRR4302534.3949100/1‑94 (MQ=60)

                                            AGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTC                                                   <  SRR4302534.5445384/94‑1 (MQ=60)

                                            AGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTC                                                   >  SRR4302534.665533/1‑94 (MQ=60)

                                             GAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCA                                                  <  SRR4302534.499180/94‑1 (MQ=60)

                                             GAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCA                                                  <  SRR4302534.4894493/94‑1 (MQ=60)

                                             GAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCA                                                  <  SRR4302534.3163952/94‑1 (MQ=60)

                                             GAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCA                                                  <  SRR4302534.5287670/94‑1 (MQ=60)

                                             GAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCA                                                  >  SRR4302534.3236522/1‑94 (MQ=60)

                                              AGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCAC                                                 >  SRR4302534.891394/1‑94 (MQ=60)

                                               GTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACT                                                <  SRR4302534.3644408/94‑1 (MQ=60)

                                               GTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATAGTAGTTAAAGACGTGCGTCACT                                                >  SRR4302534.2671961/1‑94 (MQ=60)

                                                TGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGAGGTGCGTCACTG                                               >  SRR4302534.4215425/1‑94 (MQ=60)

                                                TGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTG                                               <  SRR4302534.1787852/94‑1 (MQ=60)

                                                TGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTG                                               >  SRR4302534.3461286/1‑94 (MQ=60)

                                                TGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTG                                               <  SRR4302534.3647398/94‑1 (MQ=60)

                                                GGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTG                                               <  SRR4302534.4692351/94‑1 (MQ=60)

                                                 GACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGCCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGC                                              >  SRR4302534.2189977/1‑94 (MQ=60)

                                                  ACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCC                                             <  SRR4302534.3600676/94‑1 (MQ=60)

                                                   CCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCG                                            >  SRR4302534.3338089/1‑94 (MQ=60)

                                                    CGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGG                                           <  SRR4302534.87251/94‑1 (MQ=60)

                                                      CTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCCCTGCCGGAA                                         <  SRR4302534.6487196/94‑1 (MQ=60)

                                                      CTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAA                                         <  SRR4302534.3972522/94‑1 (MQ=60)

                                                       TTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAAT                                        <  SRR4302534.4368637/94‑1 (MQ=60)

                                                       TTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAAT                                        >  SRR4302534.2185244/1‑94 (MQ=60)

                                                       TTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAAT                                        <  SRR4302534.4368636/94‑1 (MQ=60)

                                                         GCGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATAT                                      <  SRR4302534.6605392/94‑1 (MQ=60)

                                                          CGCCTGCGCGACCTGTACGACCAATATGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATG                                     >  SRR4302534.3568522/1‑94 (MQ=60)

                                                          CGCCTGCGCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGCAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATG                                     >  SRR4302534.4438684/1‑94 (MQ=60)

                                                           GCCTGCTCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTTCCCGAATATGA                                    >  SRR4302534.2109453/1‑94 (MQ=60)

                                                                 GCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCA                              <  SRR4302534.533997/94‑1 (MQ=60)

                                                                 GCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCA                              >  SRR4302534.2124512/1‑94 (MQ=60)

                                                                 GCGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCA                              <  SRR4302534.527981/94‑1 (MQ=60)

                                                                  CGACCTGTCCGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGCAAATCGTAGTTAAAGACGTTGGTCACTGCCGGAATATGCAAACCAC                             >  SRR4302534.4548514/1‑94 (MQ=60)

                                                                  CGACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCAC                             <  SRR4302534.4136137/94‑1 (MQ=60)

                                                                   GACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACG                            >  SRR4302534.6195685/1‑94 (MQ=60)

                                                                   GACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACG                            >  SRR4302534.2529579/1‑94 (MQ=60)

                                                                    ACCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGC                           <  SRR4302534.1965393/94‑1 (MQ=60)

                                                                     CCTGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCG                          >  SRR4302534.1207884/1‑94 (MQ=60)

                                                                       TGTACGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCG                        <  SRR4302534.3355513/94‑1 (MQ=60)

                                                                           CGACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAA                    >  SRR4302534.3012469/1‑94 (MQ=60)

                                                                             ACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACG                  <  SRR4302534.1836071/94‑1 (MQ=60)

                                                                             ACCAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACG                  >  SRR4302534.3272781/1‑94 (MQ=60)

                                                                               CAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTC                <  SRR4302534.5168191/94‑1 (MQ=60)

                                                                               CAATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTC                >  SRR4302534.3309920/1‑94 (MQ=60)

                                                                               CAATACGGTGAACGCAATCTTCACAGTCATCGAGTAAATCGTAGTTAAAGACGTGCGTCACGGCCGGAATATGCAAACCACGCGCGGCAAAGTC                <  SRR4302534.3945530/94‑1 (MQ=60)

                                                                                AATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCG               <  SRR4302534.1917231/94‑1 (MQ=60)

                                                                                AATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCG               <  SRR4302534.183734/94‑1 (MQ=60)

                                                                                AATACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCG               <  SRR4302534.2553678/94‑1 (MQ=60)

                                                                                AATACCGTGAACGCAATCTTGACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCG               <  SRR4302534.1661100/94‑1 (MQ=60)

                                                                                  TACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGT             >  SRR4302534.6625924/1‑94 (MQ=60)

                                                                                  TACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGT             <  SRR4302534.4814774/94‑1 (MQ=60)

                                                                                  TACGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGT             <  SRR4302534.2353389/94‑1 (MQ=60)

                                                                                    CGGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGG           >  SRR4302534.1974275/1‑94 (MQ=60)

                                                                                     GGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGC          >  SRR4302534.1710596/1‑94 (MQ=60)

                                                                                     GGTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGC          <  SRR4302534.3012609/94‑1 (MQ=60)

                                                                                      GTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCA         >  SRR4302534.6189134/1‑94 (MQ=60)

                                                                                      GTGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCA         <  SRR4302534.3000024/94‑1 (MQ=60)

                                                                                       TGAACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAA        >  SRR4302534.6172257/1‑94 (MQ=60)

                                                                                         AACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAAGC      >  SRR4302534.2586486/1‑94 (MQ=60)

                                                                                          ACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCA     <  SRR4302534.5357245/94‑1 (MQ=60)

                                                                                          ACGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCA     <  SRR4302534.5017027/94‑1 (MQ=60)

                                                                                           CGCAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAG    >  SRR4302534.72170/1‑94 (MQ=60)

                                                                                             CAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAA  >  SRR4302534.494471/1‑94 (MQ=60)

ATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAA  >  NC_000913/3962134‑3962320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`