Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,650,355	T→C	F274L (TTC→CTC)	b1579 →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,650,355	0	T	C	99.6%	910.4 / NA	248	F274L (TTC→CTC)	b1579	putative transposase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (117/130); minor base G (0/1); total (117/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

GTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAT  >  NC_000913/1650265‑1650444

gTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTc                                                                                         <  1:523470/93‑1 (MQ=255)

gTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTc                                                                                         <  1:3911407/93‑1 (MQ=255)

  cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCag                                                                                       >  2:1799570/1‑93 (MQ=255)

  cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCag                                                                                       >  2:4786169/1‑93 (MQ=255)

  cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCag                                                                                       <  1:5909978/93‑1 (MQ=255)

   attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCaga                                                                                      >  2:726036/1‑93 (MQ=255)

   attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCaga                                                                                      <  1:484786/93‑1 (MQ=255)

   attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCaga                                                                                      <  1:1615830/93‑1 (MQ=255)

   attcatATGATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATCACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCaga                                                                                      <  1:484784/93‑1 (MQ=255)

     tcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagaga                                                                                    >  1:545228/1‑93 (MQ=255)

      catCTTATTAAGCCAGCAATCGCCGCACTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGCGCATATCATTGATGTTCACCTCagagag                                                                                   <  1:5289660/93‑1 (MQ=255)

       atCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                  <  1:4159671/93‑1 (MQ=255)

       atCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                  <  1:4997380/93‑1 (MQ=255)

       atCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                  >  2:4494496/1‑93 (MQ=255)

        tCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTa                                                                                 <  2:139191/93‑1 (MQ=255)

           tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                              >  1:967999/1‑93 (MQ=255)

            attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGc                                                                             <  1:1081783/93‑1 (MQ=255)

              taAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcag                                                                           <  1:4542294/93‑1 (MQ=255)

              taAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcag                                                                           >  2:3737225/1‑93 (MQ=255)

                aGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaa                                                                         <  1:3565399/93‑1 (MQ=255)

                  ccAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaaca                                                                       <  2:4391403/93‑1 (MQ=255)

                   cAGCAATCGACGCTCTCCGGAGTCAGATAACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacag                                                                      <  2:3098069/93‑1 (MQ=255)

                    aGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacaga                                                                     >  1:277643/1‑93 (MQ=255)

                       aaTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaaa                                                                  <  1:2465939/93‑1 (MQ=255)

                       aaTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaaa                                                                  >  2:6137279/1‑93 (MQ=255)

                        aTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaaaa                                                                 <  2:3219440/93‑1 (MQ=255)

                        aTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaaaa                                                                 <  1:1822227/93‑1 (MQ=255)

                         tCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAc                                                                <  2:6120566/93‑1 (MQ=255)

                           gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaa                                                              >  2:4909918/1‑93 (MQ=255)

                            aCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaa                                                             >  2:1039685/1‑93 (MQ=255)

                             cGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaa                                                            <  2:910553/93‑1 (MQ=255)

                              gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGCAAAACaaaaa                                                           <  1:4071912/93‑1 (MQ=255)

                              gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaaa                                                           >  1:121951/1‑93 (MQ=255)

                              gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaaa                                                           <  2:5642342/93‑1 (MQ=255)

                               ctctCCGGGGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAt                                                          <  1:5129795/93‑1 (MQ=255)

                               ctctCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAt                                                          >  2:343375/1‑93 (MQ=255)

                                 ctcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGc                                                        <  2:5248019/93‑1 (MQ=255)

                                  tcCGGAGTCAGATGACATTAGCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCa                                                       <  1:69024/93‑1 (MQ=255)

                                  tcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCa                                                       <  1:6129855/93‑1 (MQ=255)

                                    cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAcc                                                     >  1:5824485/1‑93 (MQ=255)

                                     ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCt                                                    <  1:4458166/93‑1 (MQ=255)

                                      gAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATTCACCtt                                                   <  2:5015470/93‑1 (MQ=255)

                                      gAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtt                                                   <  2:4525520/93‑1 (MQ=255)

                                      gAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtt                                                   >  1:3012047/1‑93 (MQ=255)

                                      gAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtt                                                   <  1:4792955/93‑1 (MQ=255)

                                       aGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttt                                                  <  2:1916461/93‑1 (MQ=255)

                                       aGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttt                                                  <  2:5284048/93‑1 (MQ=255)

                                        gTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTg                                                 <  2:1663887/93‑1 (MQ=255)

                                         tCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGt                                                <  2:1634043/93‑1 (MQ=255)

                                          cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtt                                               >  1:2811712/1‑93 (MQ=255)

                                          cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtt                                               >  1:1025795/1‑93 (MQ=255)

                                           aGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttt                                              >  1:142190/1‑93 (MQ=255)

                                            gATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtttt                                             >  1:962604/1‑93 (MQ=255)

                                             aTGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttttt                                            <  2:379733/93‑1 (MQ=255)

                                             aTGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttttt                                            <  2:1868387/93‑1 (MQ=255)

                                              tGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTc                                           <  1:3829041/93‑1 (MQ=255)

                                                aCATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCaa                                         >  1:1294121/1‑93 (MQ=255)

                                                  aTTAACGAGACTGAGCACAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCCCCTTTGTTTTTCAAcc                                       <  1:5485019/93‑1 (MQ=255)

                                                   ttAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCt                                      >  1:5637943/1‑93 (MQ=255)

                                                    tAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTg                                     <  1:1398758/93‑1 (MQ=255)

                                                       cGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAg                                  >  2:5140574/1‑93 (MQ=255)

                                                        gagaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgt                                 <  2:3631476/93‑1 (MQ=255)

                                                        gagaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgt                                 <  2:882310/93‑1 (MQ=255)

                                                         agaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgtg                                <  1:1693720/93‑1 (MQ=255)

                                                          gaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAACAATGCACCTTTGTTTTTCAACCTGAAgtgt                               >  2:1215861/1‑93 (MQ=255)

                                                           aCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                              <  1:2621745/93‑1 (MQ=255)

                                                           aCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                              <  1:3183389/93‑1 (MQ=255)

                                                              gAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCTCCTTTGTTTTTCAACCTGAAGTGTCAGc                           <  2:2688819/93‑1 (MQ=255)

                                                                 cAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgag                        >  1:5402491/1‑93 (MQ=255)

                                                                  aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgaga                       <  2:5571550/93‑1 (MQ=255)

                                                                   aaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGagag                      <  2:2165204/93‑1 (MQ=255)

                                                                    agagCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGt                     <  1:213001/93‑1 (MQ=255)

                                                                      agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaa                   >  2:4323232/1‑93 (MQ=255)

                                                                      agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaa                   <  1:5348726/93‑1 (MQ=255)

                                                                      agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaa                   >  1:897014/1‑93 (MQ=255)

                                                                      agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaa                   >  2:3296001/1‑93 (MQ=255)

                                                                       gCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaa                  >  2:2974971/1‑93 (MQ=255)

                                                                       gCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaa                  >  2:5451900/1‑93 (MQ=255)

                                                                        cATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaa                 <  2:5968627/93‑1 (MQ=255)

                                                                        cATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaa                 <  1:5798824/93‑1 (MQ=255)

                                                                        cATATCATTGACGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaa                 <  2:5968629/93‑1 (MQ=255)

                                                                          tatCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaaa               >  2:4713967/1‑93 (MQ=255)

                                                                            tCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAtt             >  1:2018592/1‑93 (MQ=255)

                                                                             cATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTa            <  2:2022670/93‑1 (MQ=255)

                                                                              aTTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt           >  1:882803/1‑93 (MQ=255)

                                                                               ttGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATg          >  2:4015335/1‑93 (MQ=255)

                                                                               ttGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATg          <  2:260527/93‑1 (MQ=255)

                                                                                tGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATgg         <  1:5419206/93‑1 (MQ=255)

                                                                                tGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATgg         >  1:568526/1‑93 (MQ=255)

                                                                                 gATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGt        >  1:2255038/1‑93 (MQ=255)

                                                                                 gATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGt        >  2:1508042/1‑93 (MQ=255)

                                                                                  aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTg       >  2:2485910/1‑93 (MQ=255)

                                                                                  aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTg       <  1:3358146/93‑1 (MQ=255)

                                                                                   tGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGa      >  1:4329822/1‑93 (MQ=255)

                                                                                    gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc     <  1:3834402/93‑1 (MQ=255)

                                                                                    gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc     <  1:501632/93‑1 (MQ=255)

                                                                                    gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc     >  1:4126950/1‑93 (MQ=255)

                                                                                     ttCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAcc    >  2:4106796/1‑93 (MQ=255)

                                                                                     ttCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAcc    >  1:3100881/1‑93 (MQ=255)

                                                                                      tCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCa   >  1:2962777/1‑93 (MQ=255)

GTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAT  >  NC_000913/1650265‑1650444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTA  >  NC_000913/1650263‑1650448

GGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCT                                                                                              <  SRR4302532.3853807/94‑1 (MQ=60)

 GGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTC                                                                                             >  SRR4302532.2695706/1‑94 (MQ=60)

 GGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTC                                                                                             >  SRR4302532.1742182/1‑94 (MQ=60)

  GTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCA                                                                                            <  SRR4302532.4043264/94‑1 (MQ=60)

  GTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCA                                                                                            >  SRR4302532.5468509/1‑94 (MQ=60)

  GTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCA                                                                                            <  SRR4302532.541737/94‑1 (MQ=60)

  GTCATTCATCTTATTAAGCCAGAAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCA                                                                                            <  SRR4302532.5939631/94‑1 (MQ=60)

   gtaatcaatctaattaggcaagaaatcgccgcccCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAG                                                                                           <  SRR4302532.1122886/60‑1 (MQ=60)

   TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAG                                                                                           <  SRR4302532.4476874/94‑1 (MQ=60)

   TCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAG                                                                                           >  SRR4302532.1480552/1‑94 (MQ=60)

    CATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGA                                                                                          <  SRR4302532.6114649/94‑1 (MQ=60)

     ATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAG                                                                                         <  SRR4302532.501715/94‑1 (MQ=60)

     ATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAG                                                                                         <  SRR4302532.3086421/94‑1 (MQ=60)

     ATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAG                                                                                         <  SRR4302532.501714/94‑1 (MQ=60)

     ATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAG                                                                                         <  SRR4302532.1670516/94‑1 (MQ=60)

     ATTCATATGATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATCACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAG                                                                                         <  SRR4302532.501713/94‑1 (MQ=60)

      TTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGA                                                                                        >  SRR4302532.376854/1‑94 (MQ=60)

      TTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGA                                                                                        >  SRR4302532.564226/1‑94 (MQ=60)

       tccgCTTATTAAGCCAGCAATCGACGCTCTACGGAGTCAGATGACATGAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAG                                                                                       <  SRR4302532.5269648/90‑1 (MQ=60)

        CATCTTATTAAGCCAGCAATCGCCGCACTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGCGCATATCATTGATGTTCACCTCAGAGAGT                                                                                      <  SRR4302532.5472195/94‑1 (MQ=60)

        CATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGT                                                                                      <  SRR4302532.1426427/94‑1 (MQ=60)

         ATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTA                                                                                     <  SRR4302532.5168672/94‑1 (MQ=60)

         ATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTA                                                                                     >  SRR4302532.219923/1‑94 (MQ=60)

         ATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTA                                                                                     <  SRR4302532.4299190/94‑1 (MQ=60)

         ATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTA                                                                                     >  SRR4302532.4713608/1‑94 (MQ=60)

          TCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTAT                                                                                    <  SRR4302532.999483/94‑1 (MQ=60)

           CTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCACAGAGTACG                                                                                   <  SRR4302532.3985275/94‑1 (MQ=60)

           CTTATTAAGCCAGCAATCGACGCTCTCCGGAGACAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGTTGTTCACATCAGAGAGTATG                                                                                   >  SRR4302532.1451596/1‑94 (MQ=60)

            TTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGG                                                                                  >  SRR4302532.1001029/1‑94 (MQ=60)

             TATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGC                                                                                 <  SRR4302532.4978584/94‑1 (MQ=60)

              ATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCA                                                                                <  SRR4302532.1441173/94‑1 (MQ=60)

              ATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCA                                                                                <  SRR4302532.6059889/94‑1 (MQ=60)

              ATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCA                                                                                <  SRR4302532.1118034/94‑1 (MQ=60)

                TAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGA                                                                              <  SRR4302532.4697335/94‑1 (MQ=60)

                  AGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC                                                                            >  SRR4302532.4359878/1‑94 (MQ=60)

                  AGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC                                                                            <  SRR4302532.3684687/94‑1 (MQ=60)

                    CCAGCAATCGACGCTCTCCGGAGTCAGATTACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACGTCAGAGAGTATGGCAGAACAG                                                                          <  SRR4302532.102053/94‑1 (MQ=60)

                    CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAG                                                                          >  SRR4302532.5201166/1‑94 (MQ=60)

                     CAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGA                                                                         >  SRR4302532.287253/1‑94 (MQ=60)

                       GCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAA                                                                       <  SRR4302532.5812457/94‑1 (MQ=60)

                       GCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAA                                                                       >  SRR4302532.3128513/1‑94 (MQ=60)

                        CAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAA                                                                      >  SRR4302532.5591264/1‑94 (MQ=60)

                         AATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAA                                                                     <  SRR4302532.2548024/94‑1 (MQ=60)

                          ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC                                                                    <  SRR4302532.1882935/94‑1 (MQ=60)

                          ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC                                                                    <  SRR4302532.1882934/94‑1 (MQ=60)

                          ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC                                                                    >  SRR4302532.6147610/1‑94 (MQ=60)

                          ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC                                                                    >  SRR4302532.2738488/1‑94 (MQ=60)

                            CGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA                                                                  <  SRR4302532.3614480/94‑1 (MQ=60)

                             GACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAA                                                                 >  SRR4302532.4981790/1‑94 (MQ=60)

                             GACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAA                                                                 <  SRR4302532.959701/94‑1 (MQ=60)

                               CGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAA                                                               >  SRR4302532.126097/1‑94 (MQ=60)

                               CGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAA                                                               >  SRR4302532.1839868/1‑94 (MQ=60)

                                GCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGCAAAACAAAAAT                                                              <  SRR4302532.4208671/94‑1 (MQ=60)

                                GCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAT                                                              <  SRR4302532.3360038/94‑1 (MQ=60)

                                GCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAT                                                              >  SRR4302532.6046267/1‑94 (MQ=60)

                                 CTCTCCGGGGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATG                                                             <  SRR4302532.5306262/94‑1 (MQ=60)

                                   CTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA                                                           <  SRR4302532.913736/94‑1 (MQ=60)

                                    TCCGGAGTCAGATGACATTAGCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAC                                                          <  SRR4302532.71498/94‑1 (MQ=60)

                                    TCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAC                                                          <  SRR4302532.6341366/94‑1 (MQ=60)

                                     CCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACC                                                         >  SRR4302532.6026735/1‑94 (MQ=60)

                                       GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTT                                                       <  SRR4302532.4610135/94‑1 (MQ=60)

                                       GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTT                                                       >  SRR4302532.3111481/1‑94 (MQ=60)

                                        GAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTT                                                      <  SRR4302532.4958033/94‑1 (MQ=60)

                                         AGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGTAAAACAAAAATGCATTTTTG                                                     >  SRR4302532.1965143/1‑94 (MQ=60)

                                         AGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTG                                                     <  SRR4302532.5512258/94‑1 (MQ=60)

                                         AGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTG                                                     <  SRR4302532.649695/94‑1 (MQ=60)

                                         AGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTG                                                     <  SRR4302532.2906528/94‑1 (MQ=60)

                                          GTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGT                                                    >  SRR4302532.226836/1‑94 (MQ=60)

                                           TCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTT                                                   >  SRR4302532.1082962/1‑94 (MQ=60)

                                           TCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTT                                                   >  SRR4302532.2905846/1‑94 (MQ=60)

                                           TCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTT                                                   >  SRR4302532.3728609/1‑94 (MQ=60)

                                           TCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTT                                                   >  SRR4302532.1060504/1‑94 (MQ=60)

                                            CAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTT                                                  >  SRR4302532.146986/1‑94 (MQ=60)

                                             AGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTT                                                 >  SRR4302532.742654/1‑94 (MQ=60)

                                             AGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTT                                                 >  SRR4302532.995449/1‑94 (MQ=60)

                                             AGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTT                                                 <  SRR4302532.3963715/94‑1 (MQ=60)

                                              GATGTTATTAACGAGACTGAGCATAGAGCATATCATTGATGTTCTCCTCAGAGAGCATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTT                                                >  SRR4302532.5851229/1‑94 (MQ=60)

                                              GATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTT                                                <  SRR4302532.1242076/94‑1 (MQ=60)

                                                TGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCA                                              <  SRR4302532.3958311/94‑1 (MQ=60)

                                                 GACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCGCCTTTGTTTTTCAA                                             >  SRR4302532.4042904/1‑94 (MQ=60)

                                                 GACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA                                             >  SRR4302532.1337417/1‑94 (MQ=60)

                                                   CATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCATCTCAGAGAGTATGTCAGAACAGAAAAACAAAAATTGACCTTTGTTTTTCttcg                                           >  SRR4302532.4064793/1‑90 (MQ=60)

                                                    ATTAACGAGACTGAGCACAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCCCCTTTGTTTTTCAACCT                                          <  SRR4302532.5675191/94‑1 (MQ=60)

                                                    ATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCT                                          >  SRR4302532.5833830/1‑94 (MQ=60)

                                                    ATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCT                                          <  SRR4302532.2439064/94‑1 (MQ=60)

                                                     TTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTG                                         <  SRR4302532.4647930/94‑1 (MQ=60)

                                                     TTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTG                                         <  SRR4302532.3276647/94‑1 (MQ=60)

                                                      TAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGA                                        <  SRR4302532.1445633/94‑1 (MQ=60)

                                                       AACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA                                       <  SRR4302532.2970924/94‑1 (MQ=60)

                                                        ACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAG                                      <  SRR4302532.5659909/94‑1 (MQ=60)

                                                        ACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAG                                      <  SRR4302532.5550276/94‑1 (MQ=60)

                                                         CGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGGACCTTTGTTTTTCAACCTGAAGT                                     >  SRR4302532.4637269/1‑94 (MQ=60)

                                                         CGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGT                                     >  SRR4302532.1291291/1‑94 (MQ=60)

                                                         CGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGCATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGT                                     >  SRR4302532.5114877/1‑94 (MQ=60)

                                                          GAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTG                                    >  SRR4302532.5043358/1‑94 (MQ=60)

                                                          GAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTG                                    >  SRR4302532.1167272/1‑94 (MQ=60)

                                                           AGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGT                                   <  SRR4302532.1750906/94‑1 (MQ=60)

                                                           AGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGT                                   >  SRR4302532.4272170/1‑94 (MQ=60)

                                                             ACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGaac                                 <  SRR4302532.5433360/94‑4 (MQ=60)

                                                             ACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCA                                 <  SRR4302532.2709526/94‑1 (MQ=60)

                                                             ACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCA                                 <  SRR4302532.3289099/94‑1 (MQ=60)

                                                              CTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG                                >  SRR4302532.5961022/1‑94 (MQ=60)

                                                              CTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG                                >  SRR4302532.2190313/1‑94 (MQ=60)

                                                               TGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGC                               <  SRR4302532.2893589/94‑1 (MQ=60)

                                                               TGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGGGTCAGC                               >  SRR4302532.5616195/1‑94 (MQ=60)

                                                                  GCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAG                            >  SRR4302532.5589673/1‑94 (MQ=60)

                                                                      AGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTA                        >  SRR4302532.1570825/1‑94 (MQ=60)

                                                                      AGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTA                        >  SRR4302532.6001042/1‑94 (MQ=60)

                                                                      AGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTA                        <  SRR4302532.5736779/94‑1 (MQ=60)

                                                                      AGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTA                        <  SRR4302532.220255/94‑1 (MQ=60)

                                                                       GAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAA                       >  SRR4302532.927613/1‑94 (MQ=60)

                                                                       GAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAA                       <  SRR4302532.3402740/94‑1 (MQ=60)

                                                                        AGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAA                      <  SRR4302532.5534281/94‑1 (MQ=60)

                                                                        AGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAA                      >  SRR4302532.422268/1‑94 (MQ=60)

                                                                         GCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAA                     <  SRR4302532.3184157/94‑1 (MQ=60)

                                                                          CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAA                    <  SRR4302532.6000366/94‑1 (MQ=60)

                                                                           ATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGATTAAAAAA                   >  SRR4302532.2761736/1‑94 (MQ=60)

                                                                           ATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAA                   >  SRR4302532.6358245/1‑94 (MQ=60)

                                                                           ATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAA                   >  SRR4302532.4381589/1‑94 (MQ=60)

                                                                             ATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATT                 >  SRR4302532.2085890/1‑94 (MQ=60)

                                                                             ATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATT                 <  SRR4302532.971295/94‑1 (MQ=60)

                                                                             ATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATT                 >  SRR4302532.5378977/1‑94 (MQ=60)

                                                                               CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT               >  SRR4302532.912984/1‑94 (MQ=60)

                                                                               CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT               <  SRR4302532.866863/94‑1 (MQ=60)

                                                                                 TTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGG             >  SRR4302532.588372/1‑94 (MQ=60)

                                                                                 TTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGG             >  SRR4302532.5181648/1‑94 (MQ=60)

                                                                                 TTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGG             <  SRR4302532.5857331/94‑1 (MQ=60)

                                                                                  TGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGT            <  SRR4302532.5607033/94‑1 (MQ=60)

                                                                                  TGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGT            >  SRR4302532.2329895/1‑94 (MQ=60)

                                                                                    ATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA          <  SRR4302532.3470319/94‑1 (MQ=60)

                                                                                    ATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA          >  SRR4302532.4476099/1‑94 (MQ=60)

                                                                                     TGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAC         >  SRR4302532.5614724/1‑94 (MQ=60)

                                                                                     TGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAC         >  SRR4302532.4265431/1‑94 (MQ=60)

                                                                                      GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC        >  SRR4302532.3203713/1‑94 (MQ=60)

                                                                                      GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC        <  SRR4302532.2352524/94‑1 (MQ=60)

                                                                                      GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC        <  SRR4302532.519174/94‑1 (MQ=60)

                                                                                      GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC        <  SRR4302532.3963866/94‑1 (MQ=60)

                                                                                       TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCA       >  SRR4302532.3060903/1‑94 (MQ=60)

                                                                                       TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCA       <  SRR4302532.5570020/94‑1 (MQ=60)

                                                                                       TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCA       >  SRR4302532.4235858/1‑94 (MQ=60)

                                                                                         CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATT     >  SRR4302532.1126926/1‑94 (MQ=60)

                                                                                         CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATT     >  SRR4302532.1838359/1‑94 (MQ=60)

                                                                                          ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTT    >  SRR4302532.2425247/1‑94 (MQ=60)

                                                                                          ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTT    <  SRR4302532.4549898/94‑1 (MQ=60)

                                                                                            CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTA  <  SRR4302532.2046320/94‑1 (MQ=60)

GGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTA  >  NC_000913/1650263‑1650448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`