Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,072,030 T→A C824S (TGC→AGC)  b2000 → outer membrane fluffing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,072,0300TA100.0% 689.1 / NA 189C824S (TGC→AGC) b2000outer membrane fluffing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (98/91);  total (98/91)

Only 100 of 211 total aligned reads displayed.

CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG  >  NC_000913/2071941‑2072119
                                                                                         |                                                                                         
cgGGGGTTTATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        <  2:5123968/93‑1 (MQ=255)
cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        >  1:4890984/1‑93 (MQ=255)
 gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       >  1:4572106/1‑93 (MQ=255)
 gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       <  1:5706659/93‑1 (MQ=255)
 gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       <  1:2166613/93‑1 (MQ=255)
   gggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTgg                                                                                     >  1:3553501/1‑93 (MQ=255)
   gggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTgg                                                                                     <  2:1701510/93‑1 (MQ=255)
    ggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg                                                                                    <  2:18916/93‑1 (MQ=255)
    ggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg                                                                                    <  2:3053762/93‑1 (MQ=255)
    ggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg                                                                                    <  2:4897999/93‑1 (MQ=255)
    ggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg                                                                                    >  2:5165510/1‑93 (MQ=255)
    ggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCAGGCACGGTCCGGGATGATGCCGGCAGCCTggg                                                                                    >  2:6023728/1‑93 (MQ=255)
     gTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGc                                                                                   >  2:5078863/1‑93 (MQ=255)
      tataTGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGCCGGCTCCCCTGCCGGCACGGTCCGGGCTGATGCCGGCCGCCTGGGCg                                                                                  >  1:2598876/1‑93 (MQ=255)
      tataTGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg                                                                                  >  2:4583816/1‑93 (MQ=255)
      tataTGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg                                                                                  <  1:1368299/93‑1 (MQ=255)
       atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGGAGACTGGGCgg                                                                                 >  1:264972/1‑93 (MQ=255)
        tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                <  2:3920612/93‑1 (MQ=255)
        tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                <  2:371656/93‑1 (MQ=255)
        tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                <  1:5844634/93‑1 (MQ=255)
        tatGGCGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCCTGCCGGCACAGGCCGGGATGATGGCGGCAGCCTGGGCggg                                                                                >  1:3624135/1‑92 (MQ=255)
         atGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGAt                                                                               <  2:6064441/93‑1 (MQ=255)
          tGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATa                                                                              >  1:4018709/1‑93 (MQ=255)
          tGGTGCAGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATa                                                                              <  1:1077298/93‑1 (MQ=255)
              gctgctGGCCATTCTTCCTTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTg                                                                          <  1:1120695/93‑1 (MQ=255)
              gctgctGCCCCTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTg                                                                          <  1:771498/93‑1 (MQ=255)
               ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa                                                                         >  2:1800305/1‑93 (MQ=255)
                tgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGaa                                                                        >  2:131711/1‑93 (MQ=255)
                    ggCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTg                                                                    >  2:1450950/1‑93 (MQ=255)
                     gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGGTACCTGAATCTgg                                                                   >  1:1252249/1‑93 (MQ=255)
                     gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTgg                                                                   >  2:4658838/1‑93 (MQ=255)
                     gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTgg                                                                   <  1:867246/93‑1 (MQ=255)
                       cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTa                                                                 >  1:4958424/1‑93 (MQ=255)
                       cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTa                                                                 >  2:3564362/1‑93 (MQ=255)
                       cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTa                                                                 >  1:2352635/1‑93 (MQ=255)
                        aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATCCCTGAATCTGGTac                                                                >  2:2013961/1‑93 (MQ=255)
                        aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTgggtc                                                                >  1:5640941/1‑90 (MQ=255)
                        aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTac                                                                >  2:2419907/1‑93 (MQ=255)
                        aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTac                                                                >  2:4057159/1‑93 (MQ=255)
                         ttcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTaca                                                               >  1:482221/1‑93 (MQ=255)
                         ttcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTaca                                                               >  1:5238835/1‑93 (MQ=255)
                           cttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca                                                             <  2:1920665/93‑1 (MQ=255)
                           cttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca                                                             <  2:4028482/93‑1 (MQ=255)
                            ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac                                                            >  1:501157/1‑93 (MQ=255)
                              ccGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGt                                                          >  2:4502836/1‑93 (MQ=255)
                               cGTTGATGTTAAGGATGATGGCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGTCAGCCTGGGCGGATACCTGAATCTGGTACACACGtc                                                         <  1:6036780/93‑1 (MQ=255)
                               cGTTGATGTTAAGGATGATGCCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtc                                                         <  2:570166/93‑1 (MQ=255)
                               cGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtc                                                         <  2:4940170/93‑1 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        <  1:1350951/93‑1 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        <  1:4171120/93‑1 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        >  1:4344774/1‑93 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGGCCGGGAGGAGGCCGGCGGCCTGGGCGGATACCTGAATCTGGGACACACGtcc                                                        >  2:3073844/1‑93 (MQ=255)
                                cttGAAGTTAAGGATGATGACGGCCCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        <  1:4514894/92‑1 (MQ=255)
                                 ttGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACCCACGtcct                                                       >  1:5565971/1‑93 (MQ=255)
                                 ttGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcct                                                       >  1:5391073/1‑93 (MQ=255)
                                 ttGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcct                                                       >  1:5134202/1‑93 (MQ=255)
                                  tGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGGACACACGtcctc                                                      >  1:1690079/1‑93 (MQ=255)
                                   gATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGGTGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtgctcc                                                     >  1:4413533/1‑93 (MQ=255)
                                   gATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc                                                     >  2:430015/1‑93 (MQ=255)
                                   gATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc                                                     <  2:2541923/93‑1 (MQ=255)
                                     tGTTAAGGACGATGACGGCTCCCGTGGCGGCACGGACCGGGATGATGCCGGCAGTCTGGGCGGAGACCTGCATCTGGTAAACACGTCCCCCgc                                                   >  1:5292213/1‑92 (MQ=255)
                                        tAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCt                                                <  1:3867685/93‑1 (MQ=255)
                                           ggATGATGTCGGCGCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCGTGGGCGGATTCCTGAATCTGGTACACACGTCCTCCGGCCtgtg                                             <  1:2440525/93‑1 (MQ=255)
                                           ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgtg                                             <  2:8246/93‑1 (MQ=255)
                                           ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgtg                                             >  2:5311267/1‑93 (MQ=255)
                                              tgatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGc                                          >  1:2751269/1‑93 (MQ=255)
                                              tgatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGc                                          >  1:1992186/1‑93 (MQ=255)
                                               gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  2:5883490/93‑1 (MQ=255)
                                               gatgaCGGCGCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  1:4410910/93‑1 (MQ=255)
                                                atgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTg                                        <  2:3326314/93‑1 (MQ=255)
                                                 tgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGa                                       <  1:4156169/93‑1 (MQ=255)
                                                 tgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGa                                       >  2:107001/1‑93 (MQ=255)
                                                     ggCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAtt                                   >  2:6137818/1‑93 (MQ=255)
                                                     ggCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAtt                                   <  1:2917030/93‑1 (MQ=255)
                                                      gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtg                                  >  2:3551105/1‑93 (MQ=255)
                                                      gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtg                                  >  1:4766433/1‑93 (MQ=255)
                                                      gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtg                                  >  1:1708502/1‑93 (MQ=255)
                                                        tCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgtg                                >  2:2179016/1‑93 (MQ=255)
                                                        tCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgtg                                >  1:5603500/1‑93 (MQ=255)
                                                        tCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgtg                                >  1:3940186/1‑93 (MQ=255)
                                                         cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgg                               <  2:3953520/93‑1 (MQ=255)
                                                         cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgg                               <  1:3474173/93‑1 (MQ=255)
                                                          ccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgga                              >  1:3398458/1‑92 (MQ=255)
                                                           cGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATGTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGcc                             >  2:5436643/1‑92 (MQ=255)
                                                              gCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAg                          <  1:3091324/93‑1 (MQ=255)
                                                               ccGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg                         <  1:1779313/93‑1 (MQ=255)
                                                                 ggCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGa                       >  1:4400183/1‑93 (MQ=255)
                                                                 ggCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGa                       >  2:3305861/1‑93 (MQ=255)
                                                                  gCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGaa                      <  2:474400/93‑1 (MQ=255)
                                                                         ccGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCca               >  1:2697308/1‑93 (MQ=255)
                                                                          cGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCcac              <  2:1196660/93‑1 (MQ=255)
                                                                           gggATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCAGCcaca             >  2:1391938/1‑93 (MQ=255)
                                                                             gatgatGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGAGGCACAGGGAACCCGCCACAGc           >  2:1344267/1‑93 (MQ=255)
                                                                              atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCa          <  1:2098453/93‑1 (MQ=255)
                                                                              atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCa          >  2:789952/1‑93 (MQ=255)
                                                                               tgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAt         >  1:1030201/1‑93 (MQ=255)
                                                                                 atgCCGGCAGCTGGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGa       <  1:1142238/93‑1 (MQ=255)
                                                                                  tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGaa      >  1:1987249/1‑93 (MQ=255)
                                                                                     cGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGc   <  2:350428/93‑1 (MQ=255)
                                                                                      ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCg  >  2:2454780/1‑93 (MQ=255)
                                                                                         |                                                                                         
CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG  >  NC_000913/2071941‑2072119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  NC_000913/2071937‑2072123
                                                                                             |                                                                                             
ACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCA                                                                                               <  SRR4302532.2610517/94‑1 (MQ=60)
   GCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCC                                                                                            >  SRR4302532.5059074/1‑94 (MQ=60)
    CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCT                                                                                           <  SRR4302532.4020706/94‑1 (MQ=60)
    CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCT                                                                                           <  SRR4302532.4561495/94‑1 (MQ=60)
    CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCT                                                                                           >  SRR4302532.4728040/1‑94 (MQ=60)
     GGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTG                                                                                          <  SRR4302532.2239080/94‑1 (MQ=60)
     GGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTG                                                                                          <  SRR4302532.5905616/94‑1 (MQ=60)
      GGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGG                                                                                         >  SRR4302532.3672398/1‑94 (MQ=60)
       GGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGG                                                                                        <  SRR4302532.2760395/94‑1 (MQ=60)
         GTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGCCGGCTCCCCTGCCGGCACGGTCCGGGCTGATGCCGGCCGCCTGGGCG                                                                                      >  SRR4302532.2685759/1‑94 (MQ=60)
          TATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGGAGACTGGGCGG                                                                                     >  SRR4302532.274149/1‑94 (MQ=60)
          TATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGG                                                                                     <  SRR4302532.1414131/94‑1 (MQ=60)
           ATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGA                                                                                    >  SRR4302532.5171556/1‑94 (MQ=60)
           ATATGGCGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCCTGCCGGCACAGGCCGGGATGATGGCGGCAGCCTGGGCGGG                                                                                    >  SRR4302532.3745569/1‑94 (MQ=60)
            TATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGAT                                                                                   <  SRR4302532.6047469/94‑1 (MQ=60)
            TATGGTCCTGCTGGCGATTGTTCCGTTGATGTCAAGAATGATGACGGCTCCCGTGCCGCCATGGTCCGGGATTATGCGGGCAGCGTGGGCGGAT                                                                                   <  SRR4302532.368554/94‑1 (MQ=32)
             ATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATA                                                                                  >  SRR4302532.4154131/1‑94 (MQ=60)
              TGGTGCAGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAC                                                                                 <  SRR4302532.1113449/94‑1 (MQ=60)
               GGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGGTACC                                                                                >  SRR4302532.117440/1‑94 (MQ=60)
                  GCTGCTGGCCATTCTTCCTTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGA                                                                             <  SRR4302532.1158114/94‑1 (MQ=60)
                  GCTGCTGCCCCTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGA                                                                             <  SRR4302532.798120/94‑1 (MQ=60)
                   CTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAA                                                                            >  SRR4302532.5775364/1‑94 (MQ=60)
                      CTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGGTACCGGAATCT                                                                         >  SRR4302532.1412846/1‑94 (MQ=60)
                      CTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCT                                                                         >  SRR4302532.5238837/1‑94 (MQ=60)
                       TGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTG                                                                        >  SRR4302532.1989952/1‑94 (MQ=60)
                        GGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGGTACCTGAATCTGG                                                                       >  SRR4302532.1294153/1‑94 (MQ=60)
                        GGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGG                                                                       >  SRR4302532.5603393/1‑94 (MQ=60)
                         GCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGT                                                                      <  SRR4302532.4931987/94‑1 (MQ=60)
                         GCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGT                                                                      <  SRR4302532.896980/94‑1 (MQ=60)
                          CCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTA                                                                     >  SRR4302532.2431134/1‑94 (MQ=60)
                          CCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTA                                                                     >  SRR4302532.5128514/1‑94 (MQ=60)
                           CATTCTTCCGTTGATGTTAAGGATGATGACGGCTTCCGGGCCGGCACGGTCCGGGATGATGCCGGAAGCCTGGGCGGAGACCTGAATCTGGGAC                                                                    >  SRR4302532.5877688/1‑94 (MQ=60)
                           CATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGgtc                                                                    >  SRR4302532.5837012/1‑91 (MQ=60)
                           CATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTAC                                                                    >  SRR4302532.2003771/1‑94 (MQ=60)
                           CATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTAC                                                                    <  SRR4302532.2876230/94‑1 (MQ=60)
                            ATTCTTCCGTTGATGTTCAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACA                                                                   >  SRR4302532.4309805/1‑94 (MQ=60)
                            ATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACA                                                                   >  SRR4302532.5419462/1‑94 (MQ=60)
                            ATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACA                                                                   >  SRR4302532.499043/1‑94 (MQ=60)
                              TCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACA                                                                 >  SRR4302532.5569730/1‑94 (MQ=60)
                               CTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACAC                                                                <  SRR4302532.2737656/94‑1 (MQ=60)
                               CTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACAC                                                                >  SRR4302532.518684/1‑94 (MQ=60)
                               CTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACAC                                                                <  SRR4302532.4462558/94‑1 (MQ=60)
                               CGTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACAC                                                                <  SRR4302532.4845978/94‑1 (MQ=60)
                                 TCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGT                                                              >  SRR4302532.1110700/1‑94 (MQ=60)
                                 TCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGT                                                              <  SRR4302532.5444114/94‑1 (MQ=60)
                                   CGTTGATGTTAAGGATGATGGCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGTCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCC                                                            <  SRR4302532.6245267/94‑1 (MQ=60)
                                   CGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCC                                                            <  SRR4302532.4797713/94‑1 (MQ=60)
                                   CGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCC                                                            >  SRR4302532.4491567/1‑94 (MQ=60)
                                    GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACCCACGTCCT                                                           >  SRR4302532.5759247/1‑94 (MQ=60)
                                    GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           <  SRR4302532.4311010/94‑1 (MQ=60)
                                    GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           >  SRR4302532.5577850/1‑94 (MQ=60)
                                    GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           <  SRR4302532.1396135/94‑1 (MQ=60)
                                    GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           >  SRR4302532.5310815/1‑94 (MQ=60)
                                    CTTGAAGTTAAGGATGATGACGGCCCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           <  SRR4302532.4669036/94‑1 (MQ=60)
                                     TTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGGACACACGTCCTC                                                          >  SRR4302532.1747150/1‑94 (MQ=60)
                                     TTGATGTTAAGGATGATGACGGCTCCCGGGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACATCCTC                                                          >  SRR4302532.4528131/1‑94 (MQ=60)
                                      TGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGGTGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTGCTCC                                                         >  SRR4302532.4563884/1‑94 (MQ=60)
                                       GATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCG                                                        <  SRR4302532.616523/94‑1 (MQ=60)
                                         TGTTAAGGATGATGACGGCTCGCGTGCCGGCACGGTCCGGGGTGATGCCGGCAGCCCGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGC                                                      >  SRR4302532.3467389/1‑94 (MQ=60)
                                          GTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCC                                                     >  SRR4302532.2060703/1‑94 (MQ=60)
                                            TAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTG                                                   <  SRR4302532.3998212/94‑1 (MQ=60)
                                             AAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGT                                                  <  SRR4302532.5923489/94‑1 (MQ=60)
                                             AAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGT                                                  >  SRR4302532.3078179/1‑94 (MQ=60)
                                              cggatcaagaggcctaCCGTGCCGGCCCGCTCCGGCATGACGCCGGCCGCCTGGGCGGATACCTGAATCTGATACACACGTCCTCCCGCCTGTG                                                 <  SRR4302532.2578377/78‑1 (MQ=47)
                                              AGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGGTGATGCCGGCAGCCTGGGCGGATACCTGCATCTGGTACACACGTCCGCCCGCCTGTG                                                 >  SRR4302532.2562147/1‑94 (MQ=60)
                                              AGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTG                                                 <  SRR4302532.3745542/94‑1 (MQ=60)
                                              AGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTG                                                 >  SRR4302532.4992759/1‑94 (MQ=60)
                                              AGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTG                                                 <  SRR4302532.2461053/94‑1 (MQ=60)
                                               GGATGATGTCGGCGCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCGTGGGCGGATTCCTGAATCTGGTACACACGTCCTCCGGCCTGTGG                                                <  SRR4302532.2521676/94‑1 (MQ=60)
                                               GGATGATGACAGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGG                                                <  SRR4302532.421352/94‑1 (MQ=60)
                                                 ATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGC                                              <  SRR4302532.3586204/94‑1 (MQ=60)
                                                 ATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGC                                              >  SRR4302532.2843674/1‑94 (MQ=60)
                                                 ATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGC                                              >  SRR4302532.2058465/1‑94 (MQ=60)
                                                  TGAGGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCT                                             <  SRR4302532.4680181/94‑1 (MQ=60)
                                                   GATGACGGCGCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTG                                            <  SRR4302532.4561176/94‑1 (MQ=60)
                                                     TGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGAC                                          <  SRR4302532.4295592/94‑1 (MQ=60)
                                                     TGACGGCTCCCGTGCCGGCACGGCCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGGTGAC                                          >  SRR4302532.643808/1‑94 (MQ=60)
                                                      GACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACA                                         <  SRR4302532.3659088/94‑1 (MQ=60)
                                                        CGGCTCCCGTGCCGGCGCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATT                                       <  SRR4302532.1789684/94‑1 (MQ=60)
                                                         GGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTGCTCCGGCCTGTGGGCTGACATTG                                      >  SRR4302532.3930112/1‑94 (MQ=60)
                                                         GGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTG                                      >  SRR4302532.1766099/1‑94 (MQ=60)
                                                         GGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTG                                      >  SRR4302532.4929874/1‑94 (MQ=60)
                                                         GGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTG                                      >  SRR4302532.2207816/1‑94 (MQ=60)
                                                         GGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTG                                      <  SRR4302532.3014216/94‑1 (MQ=60)
                                                           CTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTG                                    >  SRR4302532.4072958/1‑94 (MQ=60)
                                                           CTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTG                                    >  SRR4302532.5798004/1‑94 (MQ=60)
                                                            TCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGG                                   <  SRR4302532.235197/94‑1 (MQ=60)
                                                            TCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGG                                   <  SRR4302532.2836269/94‑1 (MQ=60)
                                                             CCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGC                                  <  SRR4302532.3590531/94‑1 (MQ=60)
                                                             CCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGA                                  >  SRR4302532.3512223/1‑94 (MQ=60)
                                                              CCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGTCATTGTGGCA                                 >  SRR4302532.4084763/1‑94 (MQ=60)
                                                               CGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCAC                                >  SRR4302532.4140867/1‑94 (MQ=60)
                                                               CGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCAC                                >  SRR4302532.2840486/1‑94 (MQ=60)
                                                                  GCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGG                             <  SRR4302532.3193770/94‑1 (MQ=60)
                                                                   CCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGG                            >  SRR4302532.1346078/1‑94 (MQ=60)
                                                                   CCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGG                            <  SRR4302532.1838846/94‑1 (MQ=60)
                                                                    CGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGA                           >  SRR4302532.4549957/1‑94 (MQ=60)
                                                                    CGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGA                           >  SRR4302532.4549956/1‑94 (MQ=60)
                                                                       CACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACC                        <  SRR4302532.5803226/94‑1 (MQ=60)
                                                                         CGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCG                      <  SRR4302532.4223811/94‑1 (MQ=60)
                                                                         CGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCG                      >  SRR4302532.5341439/1‑94 (MQ=60)
                                                                          GGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGC                     >  SRR4302532.5062545/1‑94 (MQ=60)
                                                                           GTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGTGAACCCGCC                    >  SRR4302532.2563572/1‑94 (MQ=60)
                                                                            TCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCA                   >  SRR4302532.2787906/1‑94 (MQ=60)
                                                                             CCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCAC                  >  SRR4302532.2056816/1‑94 (MQ=60)
                                                                              CGGGAGGAGGCCGGCAGCCGGGGCGGATACCTGAATCTGGTACACCCGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACA                 <  SRR4302532.4181631/94‑1 (MQ=60)
                                                                                 GATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCA              >  SRR4302532.129069/1‑94 (MQ=60)
                                                                                  ATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAT             >  SRR4302532.1065008/1‑94 (MQ=60)
                                                                                  ATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAT             <  SRR4302532.2168459/94‑1 (MQ=60)
                                                                                    GATGCGGGCAGCCTGGGCTGATCCCTGAATCTGTTACACACGCCCTCCGGCCGGTGGGCTGACATTGTGGCAAAGGGAACCCGCCACAGCATGA           <  SRR4302532.682677/94‑1 (MQ=60)
                                                                                     ATGCCGGCAGCTGGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAA          <  SRR4302532.1180335/94‑1 (MQ=60)
                                                                                     ATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAA          >  SRR4302532.2053284/1‑94 (MQ=60)
                                                                                     ATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAA          >  SRR4302532.1638862/1‑94 (MQ=60)
                                                                                       GCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAG        >  SRR4302532.1154979/1‑94 (MQ=60)
                                                                                       GCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAG        >  SRR4302532.463642/1‑94 (MQ=60)
                                                                                       GCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAG        >  SRR4302532.851691/1‑94 (MQ=60)
                                                                                        CCGGCGGCCTGGGCGGATACCTGAATCTGGTACCCACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGC       <  SRR4302532.5652522/94‑1 (MQ=60)
                                                                                        CCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGG       >  SRR4302532.1757307/1‑94 (MQ=60)
                                                                                        CCGGAAGCCTGGGCGGATCCCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGC       <  SRR4302532.893675/94‑1 (MQ=60)
                                                                                            CAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCA   >  SRR4302532.3340851/1‑94 (MQ=60)
                                                                                             GGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  <  SRR4302532.1824272/94‑1 (MQ=60)
                                                                                             AGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  SRR4302532.5541657/1‑94 (MQ=60)
                                                                                             AGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  SRR4302532.888262/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  NC_000913/2071937‑2072123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑