Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,857,315 C→T Q734* (CAG→TAG)  mutS → MutS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,857,3150CT99.1% 877.8 / ‑3.3 233Q734* (CAG→TAG) mutSMutS
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (138/93);  total (138/95)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 236 total aligned reads displayed.

GGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAG  >  NC_000913/2857225‑2857404
                                                                                          |                                                                                         
  tCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGtt                                                                                       <  1:4538877/93‑1 (MQ=255)
    tGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAc                                                                                     <  1:6103546/93‑1 (MQ=255)
    tGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAc                                                                                     <  2:4487767/93‑1 (MQ=255)
     gTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAcc                                                                                    <  2:5940755/93‑1 (MQ=255)
         cTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGgag                                                                                >  2:2613467/1‑93 (MQ=255)
         cTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGgag                                                                                >  2:5871333/1‑93 (MQ=255)
            gcgtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGaaa                                                                             <  1:51459/93‑1 (MQ=255)
              gtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGGTACCCACTATTTCGAGCTGTCCTAGCTACCGGAGaaaag                                                                           >  2:399396/1‑92 (MQ=255)
              gtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAAt                                                                           <  1:1440479/93‑1 (MQ=255)
                gggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATgg                                                                         <  2:4841826/93‑1 (MQ=255)
                gggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATgg                                                                         >  2:4622558/1‑93 (MQ=255)
                 ggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGa                                                                        <  2:4370525/93‑1 (MQ=255)
                     tgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGc                                                                    >  2:367047/1‑93 (MQ=255)
                          ggAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGc                                                               <  1:175143/93‑1 (MQ=255)
                             aaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTac                                                            >  2:1345787/1‑92 (MQ=255)
                             aaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTaa                                                            <  1:4449951/93‑1 (MQ=255)
                               aTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACg                                                          >  1:6139508/1‑93 (MQ=255)
                               aTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACg                                                          >  1:2845313/1‑93 (MQ=255)
                               aTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACg                                                          <  2:704666/93‑1 (MQ=255)
                                tCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGt                                                         <  2:2077707/93‑1 (MQ=255)
                                   ggCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTCACGTGCa                                                      >  2:392820/1‑93 (MQ=255)
                                   ggCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCa                                                      >  1:2581450/1‑93 (MQ=255)
                                      gAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtct                                                   >  1:126353/1‑93 (MQ=255)
                                      gAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtct                                                   >  2:1766877/1‑93 (MQ=255)
                                        aTAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCg                                                 >  1:891342/1‑93 (MQ=255)
                                        aTAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCg                                                 >  1:5902523/1‑93 (MQ=255)
                                        aTAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCg                                                 >  1:1658206/1‑93 (MQ=255)
                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                >  1:3446421/1‑93 (MQ=255)
                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                >  2:1250421/1‑93 (MQ=255)
                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                <  2:3286657/93‑1 (MQ=255)
                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                <  2:3099827/93‑1 (MQ=255)
                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                >  2:5183787/1‑93 (MQ=255)
                                           aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATg                                              <  2:551837/93‑1 (MQ=255)
                                           aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATg                                              >  2:4129142/1‑93 (MQ=255)
                                           aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATg                                              >  2:5025772/1‑93 (MQ=255)
                                            gATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGc                                             >  2:1714425/1‑93 (MQ=255)
                                             aTTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCa                                            >  1:4244902/1‑93 (MQ=255)
                                             aTTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCa                                            >  1:4137599/1‑93 (MQ=255)
                                              ttAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCAc                                           >  1:3041423/1‑93 (MQ=255)
                                                  ggCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       <  1:1100423/93‑1 (MQ=255)
                                                  ggCATTGACGTTATTTGCTACCCACTATTCCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       <  1:4674259/93‑1 (MQ=255)
                                                   gCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAg                                      <  2:3334136/93‑1 (MQ=255)
                                                    cATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGc                                     >  1:1950779/1‑93 (MQ=255)
                                                     aTTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGGATCTCGATGCACTGGAGCa                                    >  1:5672637/1‑93 (MQ=255)
                                                     aTTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCa                                    >  1:5025426/1‑93 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTTGAGCAc                                   >  1:5470482/1‑93 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   <  2:5045340/93‑1 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   <  1:970743/93‑1 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   >  1:2717767/1‑93 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   >  1:4500902/1‑93 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   >  1:3204728/1‑93 (MQ=255)
                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   >  1:4600103/1‑93 (MQ=255)
                                                       tGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACg                                  >  1:1008600/1‑93 (MQ=255)
                                                       tGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACg                                  >  2:2929992/1‑93 (MQ=255)
                                                         aCGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGc                                >  2:5759638/1‑93 (MQ=255)
                                                           gTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGa                              >  1:3421855/1‑93 (MQ=255)
                                                           gTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGa                              >  2:1967906/1‑93 (MQ=255)
                                                            ttATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGac                             >  1:723595/1‑93 (MQ=255)
                                                            ttATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGac                             >  2:2810817/1‑93 (MQ=255)
                                                             tATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGaca                            >  2:1019891/1‑93 (MQ=255)
                                                             tATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGaca                            >  1:3572418/1‑93 (MQ=255)
                                                             tATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGaca                            >  2:3777209/1‑93 (MQ=255)
                                                              aTTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGacac                           >  1:4092019/1‑93 (MQ=255)
                                                              aTTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGacac                           >  1:4958475/1‑93 (MQ=255)
                                                                ttGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCa                         >  2:725783/1‑93 (MQ=255)
                                                                 tGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAt                        >  2:218146/1‑93 (MQ=255)
                                                                 tGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAt                        <  2:5368689/93‑1 (MQ=255)
                                                                  gCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAtt                       <  2:5285823/93‑1 (MQ=255)
                                                                    tACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGc                     >  2:3115874/1‑93 (MQ=255)
                                                                     aCCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGcc                    <  2:1899338/93‑1 (MQ=255)
                                                                     aCCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGcc                    <  1:3903175/93‑1 (MQ=255)
                                                                     aCCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAGTGcc                    >  2:1275211/1‑93 (MQ=255)
                                                                        cACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCttt                 >  1:2304678/1‑93 (MQ=255)
                                                                         ccTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTa                <  2:1082455/92‑1 (MQ=255)
                                                                         aCTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTa                >  1:3538137/1‑93 (MQ=255)
                                                                          cTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTAt               >  2:2375822/1‑93 (MQ=255)
                                                                          cTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTAt               <  1:1584072/93‑1 (MQ=255)
                                                                           tATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATg              >  2:3435927/1‑93 (MQ=255)
                                                                           tATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATg              <  2:421471/93‑1 (MQ=255)
                                                                           tATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATg              >  1:5947191/1‑93 (MQ=255)
                                                                            aTTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGc             >  2:1437636/1‑93 (MQ=255)
                                                                            aTTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGc             <  2:2159382/93‑1 (MQ=255)
                                                                             tttCGAGCTGACCTAGTTACCGGAGACAATGGAAGTCGTCGCTAACGTGCATCTCGATGCACTGGAGAACGGCGACACCATTGCCTTTATGca            >  1:3395769/1‑93 (MQ=255)
                                                                             tttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGGGCACGGCGACACCATTGCCTTTATGca            >  2:5512559/1‑93 (MQ=255)
                                                                             tttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGca            <  2:5676565/93‑1 (MQ=255)
                                                                              ttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGcac           >  1:3858776/1‑93 (MQ=255)
                                                                              ttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGcac           >  1:238873/1‑93 (MQ=255)
                                                                              ttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGcac           >  1:3724936/1‑93 (MQ=255)
                                                                                 gAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGc        <  2:5950115/93‑1 (MQ=255)
                                                                                 gAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGc        >  1:2179508/1‑93 (MQ=255)
                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       <  1:5238502/93‑1 (MQ=255)
                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       <  1:2799649/93‑1 (MQ=255)
                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       <  1:3274795/93‑1 (MQ=255)
                                                                                   gCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCgg      >  1:1359058/1‑92 (MQ=255)
                                                                                   gCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGt      <  1:5318515/93‑1 (MQ=255)
                                                                                    gctgcccAATTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTg     <  2:5701599/89‑1 (MQ=255)
                                                                                    cTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTg     <  1:603629/93‑1 (MQ=255)
                                                                                     tGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGc    >  2:2504315/1‑93 (MQ=255)
                                                                                       aCCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAg  >  1:2900504/1‑93 (MQ=255)
                                                                                       aCCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAg  <  2:2726199/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAG  >  NC_000913/2857225‑2857404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  >  NC_000913/2857222‑2857407
                                                                                             |                                                                                            
GATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCT                                                                                              >  SRR4302532.559156/1‑94 (MQ=60)
GATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCT                                                                                              <  SRR4302532.3641357/94‑1 (MQ=60)
GATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCT                                                                                              >  SRR4302532.3542320/1‑94 (MQ=60)
 ATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTA                                                                                             >  SRR4302532.3736665/1‑94 (MQ=60)
 ATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTA                                                                                             >  SRR4302532.5576680/1‑94 (MQ=60)
  TGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGAATTGACGTTATTTGCTACCCCCGATTTCGAGCTGCCCTAG                                                                                            <  SRR4302532.3891833/94‑1 (MQ=60)
     TCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTA                                                                                         >  SRR4302532.4355188/1‑94 (MQ=60)
     TCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTA                                                                                         <  SRR4302532.4693809/94‑1 (MQ=60)
      CTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAC                                                                                        >  SRR4302532.2910290/1‑94 (MQ=60)
       TGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACC                                                                                       <  SRR4302532.6314178/94‑1 (MQ=60)
        GTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCG                                                                                      <  SRR4302532.2845171/94‑1 (MQ=60)
               GCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAA                                                                               <  SRR4302532.53288/94‑1 (MQ=60)
                CGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAAT                                                                              <  SRR4302532.5188023/94‑1 (MQ=60)
                 GTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATG                                                                             <  SRR4302532.1489027/94‑1 (MQ=60)
                  GGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGG                                                                            <  SRR4302532.2201055/94‑1 (MQ=60)
                      CGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGG                                                                        <  SRR4302532.3441368/94‑1 (MQ=60)
                      CGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGG                                                                        <  SRR4302532.95456/94‑1 (MQ=60)
                       GTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGC                                                                       <  SRR4302532.964028/94‑1 (MQ=60)
                         GCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGT                                                                     >  SRR4302532.4990848/1‑94 (MQ=60)
                          CGCGTAAAATTTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTGCCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTC                                                                    <  SRR4302532.3566096/94‑1 (MQ=60)
                          CGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTC                                                                    <  SRR4302532.6216834/94‑1 (MQ=60)
                           GCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCG                                                                   <  SRR4302532.1216975/94‑1 (MQ=60)
                           GCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCG                                                                   <  SRR4302532.5152826/94‑1 (MQ=60)
                            CGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGC                                                                  >  SRR4302532.6286355/1‑94 (MQ=60)
                             GGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCT                                                                 >  SRR4302532.4807606/1‑94 (MQ=60)
                             GGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCT                                                                 >  SRR4302532.3673632/1‑94 (MQ=60)
                             GGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCT                                                                 <  SRR4302532.181008/94‑1 (MQ=60)
                             GGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCT                                                                 >  SRR4302532.870907/1‑94 (MQ=60)
                              GAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTA                                                                >  SRR4302532.364295/1‑94 (MQ=60)
                                AAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAC                                                              >  SRR4302532.5754957/1‑94 (MQ=60)
                                AAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAC                                                              <  SRR4302532.4601618/94‑1 (MQ=60)
                                AAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAC                                                              <  SRR4302532.2543638/94‑1 (MQ=60)
                                AAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAC                                                              <  SRR4302532.4601617/94‑1 (MQ=60)
                                AAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAC                                                              >  SRR4302532.5356562/1‑94 (MQ=60)
                                AAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAC                                                              >  SRR4302532.5356563/1‑94 (MQ=60)
                                 AATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACG                                                             >  SRR4302532.2940419/1‑94 (MQ=60)
                                 AATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACG                                                             >  SRR4302532.6351293/1‑94 (MQ=60)
                                  ATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGT                                                            >  SRR4302532.6155992/1‑94 (MQ=60)
                                  ATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGG                                                            >  SRR4302532.2377894/1‑94 (MQ=60)
                                   TCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTG                                                           <  SRR4302532.4003903/94‑1 (MQ=60)
                                    CTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGGGTCGCTAACGTGC                                                          >  SRR4302532.5496213/1‑94 (MQ=60)
                                    CTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGC                                                          >  SRR4302532.3907839/1‑94 (MQ=60)
                                    CTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGC                                                          >  SRR4302532.2613461/1‑94 (MQ=60)
                                     TGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCA                                                         >  SRR4302532.2667721/1‑94 (MQ=60)
                                     TGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCA                                                         <  SRR4302532.4518242/94‑1 (MQ=60)
                                        CGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCT                                                      >  SRR4302532.130631/1‑94 (MQ=60)
                                         GAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTC                                                     >  SRR4302532.1532703/1‑94 (MQ=60)
                                          AATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCG                                                    >  SRR4302532.6106911/1‑94 (MQ=60)
                                          AATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCG                                                    >  SRR4302532.1714407/1‑94 (MQ=60)
                                          AATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCG                                                    >  SRR4302532.921782/1‑94 (MQ=60)
                                           ATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGA                                                   >  SRR4302532.4071113/1‑94 (MQ=60)
                                           ATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGA                                                   >  SRR4302532.3561868/1‑94 (MQ=60)
                                           ATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGA                                                   >  SRR4302532.1368450/1‑94 (MQ=60)
                                           ATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGA                                                   >  SRR4302532.3561867/1‑94 (MQ=60)
                                             AAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATG                                                 >  SRR4302532.5616713/1‑94 (MQ=60)
                                             AAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATG                                                 >  SRR4302532.4076306/1‑94 (MQ=60)
                                               GATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCA                                               >  SRR4302532.4387526/1‑94 (MQ=60)
                                               GATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCA                                               >  SRR4302532.4276395/1‑94 (MQ=60)
                                                ATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCAC                                              >  SRR4302532.3141777/1‑94 (MQ=60)
                                                ATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCAC                                              >  SRR4302532.3141776/1‑94 (MQ=60)
                                                 TTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACT                                             <  SRR4302532.6146594/94‑1 (MQ=60)
                                                   AAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGG                                           >  SRR4302532.1413341/1‑94 (MQ=60)
                                                    AGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGG                                          >  SRR4302532.3409839/1‑94 (MQ=60)
                                                     GGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAG                                         <  SRR4302532.1137198/94‑1 (MQ=60)
                                                     GGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAG                                         <  SRR4302532.3809838/94‑1 (MQ=60)
                                                     GGCATTGACGTTATTTGCTACCCACTATTCCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAG                                         <  SRR4302532.4833640/94‑1 (MQ=60)
                                                      GCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGC                                        >  SRR4302532.2015623/1‑94 (MQ=60)
                                                      GCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGC                                        >  SRR4302532.2389400/1‑94 (MQ=60)
                                                       CATTGGCGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCA                                       <  SRR4302532.1397139/94‑1 (MQ=60)
                                                       CATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGGATCTCGATGCACTGGAGCA                                       >  SRR4302532.5870535/1‑94 (MQ=60)
                                                       CATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCA                                       >  SRR4302532.5197796/1‑94 (MQ=60)
                                                        ATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTTGAGCAC                                      >  SRR4302532.5660132/1‑94 (MQ=60)
                                                        ATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAC                                      >  SRR4302532.4654566/1‑94 (MQ=60)
                                                        ATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAC                                      >  SRR4302532.3311139/1‑94 (MQ=60)
                                                        ATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAC                                      >  SRR4302532.4591167/1‑94 (MQ=60)
                                                        ATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAC                                      >  SRR4302532.2809168/1‑94 (MQ=60)
                                                        ATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAC                                      >  SRR4302532.4756937/1‑94 (MQ=60)
                                                         TTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACG                                     <  SRR4302532.4676110/94‑1 (MQ=60)
                                                         TTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACG                                     >  SRR4302532.1042902/1‑94 (MQ=60)
                                                         TTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACG                                     <  SRR4302532.1003857/94‑1 (MQ=60)
                                                          TGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGG                                    >  SRR4302532.3319680/1‑94 (MQ=60)
                                                          TGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGG                                    >  SRR4302532.1902721/1‑94 (MQ=60)
                                                            ACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCG                                  >  SRR4302532.3140644/1‑94 (MQ=60)
                                                            ACCTTATTTGCTACCCACTATTTCGAGCTGACCAAGTAACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCGCGGCG                                  <  SRR4302532.5592758/94‑1 (MQ=60)
                                                             CGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGA                                 >  SRR4302532.3536487/1‑94 (MQ=60)
                                                              GTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGAC                                >  SRR4302532.633458/1‑94 (MQ=60)
                                                              GTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGAC                                >  SRR4302532.748273/1‑94 (MQ=60)
                                                               TTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACA                               >  SRR4302532.3691963/1‑94 (MQ=60)
                                                               TTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACA                               <  SRR4302532.3165407/94‑1 (MQ=60)
                                                                TATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAGATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACAC                              <  SRR4302532.2992482/94‑1 (MQ=60)
                                                                TATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACAC                              >  SRR4302532.5128566/1‑94 (MQ=60)
                                                                TATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACAC                              >  SRR4302532.4229392/1‑94 (MQ=60)
                                                                 ATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACC                             >  SRR4302532.4698991/1‑94 (MQ=60)
                                                                     TCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTG                         <  SRR4302532.1690558/94‑1 (MQ=60)
                                                                      CTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGC                        >  SRR4302532.3514801/1‑94 (MQ=60)
                                                                      CTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGC                        <  SRR4302532.1777944/94‑1 (MQ=60)
                                                                      CTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGAGCATCTCGATGCACTGGGGCACTGCGACCCCTTTGC                        >  SRR4302532.5241909/1‑94 (MQ=60)
                                                                       TACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCC                       >  SRR4302532.5718254/1‑94 (MQ=60)
                                                                       TACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCC                       >  SRR4302532.2878166/1‑94 (MQ=60)
                                                                        ACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCT                      <  SRR4302532.4034800/94‑1 (MQ=60)
                                                                        ACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCT                      >  SRR4302532.1645529/1‑94 (MQ=60)
                                                                         CCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTT                     <  SRR4302532.3798009/94‑1 (MQ=60)
                                                                          CCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTT                    >  SRR4302532.1064054/1‑94 (MQ=60)
                                                                          CCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTT                    >  SRR4302532.2381302/1‑94 (MQ=60)
                                                                           CACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTA                   >  SRR4302532.3656513/1‑94 (MQ=60)
                                                                            ACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTAT                  >  SRR4302532.2192264/1‑94 (MQ=60)
                                                                             CTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATG                 >  SRR4302532.6152955/1‑94 (MQ=60)
                                                                             CTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATG                 <  SRR4302532.1637692/94‑1 (MQ=60)
                                                                               ATTTCGAGCTGACCTAGTTACCGGAGACAATGGAAGTCGTCGCTAACGTGCATCTCGATGCACTGGAGAACGGCGACACCATTGCCTTTATGCA               >  SRR4302532.3509403/1‑94 (MQ=60)
                                                                                TTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCAC              >  SRR4302532.247105/1‑94 (MQ=60)
                                                                                TTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCAC              >  SRR4302532.3989057/1‑94 (MQ=60)
                                                                                TTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCAC              >  SRR4302532.3850023/1‑94 (MQ=60)
                                                                                  TCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAG            <  SRR4302532.690719/94‑1 (MQ=60)
                                                                                  TCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAG            <  SRR4302532.733231/94‑1 (MQ=60)
                                                                                   CGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGC           >  SRR4302532.2252269/1‑94 (MQ=60)
                                                                                     AGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGT         <  SRR4302532.2893465/94‑1 (MQ=60)
                                                                                     AGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGT         <  SRR4302532.5419123/94‑1 (MQ=60)
                                                                                     AGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGT         <  SRR4302532.3383699/94‑1 (MQ=60)
                                                                                     AGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGG         >  SRR4302532.1404565/1‑94 (MQ=60)
                                                                                      GCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTG        <  SRR4302532.5502596/94‑1 (MQ=60)
                                                                                      GCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTG        <  SRR4302532.2797975/94‑1 (MQ=60)
                                                                                       CTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGC       <  SRR4302532.624721/94‑1 (MQ=60)
                                                                                        TGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCA      <  SRR4302532.601511/94‑1 (MQ=60)
                                                                                         GACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAG     >  SRR4302532.2997300/1‑94 (MQ=60)
                                                                                         GACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAG     <  SRR4302532.4699198/94‑1 (MQ=60)
                                                                                          ACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGG    >  SRR4302532.4147809/1‑94 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  <  SRR4302532.2047273/94‑1 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  <  SRR4302532.2155328/94‑1 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  >  SRR4302532.595994/1‑94 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  <  SRR4302532.1496619/94‑1 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  <  SRR4302532.2632918/94‑1 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  >  SRR4302532.302817/1‑94 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  >  SRR4302532.5196583/1‑94 (MQ=60)
                                                                                            CTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  >  SRR4302532.97339/1‑94 (MQ=60)
                                                                                             |                                                                                            
GATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGAT  >  NC_000913/2857222‑2857407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑