Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,245	C→G	G470G (GGG→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,245	0	C	G	99.2%	844.0 / NA	244	G470G (GGG→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (115/127); minor base T (1/1); total (116/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078155‑3078334

cgcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                         <  2:3659661/93‑1 (MQ=255)

    gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                     <  1:3191631/93‑1 (MQ=255)

    gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                     >  1:3120566/1‑93 (MQ=255)

     aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    <  2:736677/93‑1 (MQ=255)

     aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    <  1:828677/93‑1 (MQ=255)

     aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    <  1:364869/93‑1 (MQ=255)

      ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   >  2:5662888/1‑93 (MQ=255)

      ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   <  2:4289195/93‑1 (MQ=255)

       tCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCg                                                                                  >  2:4068658/1‑93 (MQ=255)

       tCAAACTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGtcgtcg                                                                                  >  1:4279779/1‑93 (MQ=255)

        cAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGc                                                                                 <  2:692858/93‑1 (MQ=255)

         aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                >  2:83541/1‑93 (MQ=255)

         aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGcc                                                                                >  1:1743831/1‑93 (MQ=255)

          aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                               >  1:4397753/1‑93 (MQ=255)

           ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                                              <  2:1300042/93‑1 (MQ=255)

           ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                                              <  2:4841246/93‑1 (MQ=255)

             tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            <  1:3776765/93‑1 (MQ=255)

             tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            <  2:118507/93‑1 (MQ=255)

              ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                           <  1:4032300/93‑1 (MQ=255)

              ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                           <  2:570574/93‑1 (MQ=255)

              ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                           >  1:2187690/1‑93 (MQ=255)

                tCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCg                                                                         <  1:3852599/93‑1 (MQ=255)

                  acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       <  1:2350987/93‑1 (MQ=255)

                    acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAttg                                                                     <  2:2079027/93‑3 (MQ=255)

                     cGGACTGCATGTAGACATGTTCGGGGTTACTCACAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  1:1389816/93‑1 (MQ=255)

                     cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  2:3858205/93‑1 (MQ=255)

                     cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  2:1597867/93‑1 (MQ=255)

                       gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGGTGGAgg                                                                  >  2:1963375/1‑92 (MQ=255)

                         cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                <  2:3750957/93‑1 (MQ=255)

                          gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  1:3293886/93‑1 (MQ=255)

                          gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  1:5601106/93‑1 (MQ=255)

                            cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             >  2:1255812/1‑93 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGgggg                                                            >  2:1872440/1‑93 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  1:1144702/1‑93 (MQ=255)

                              tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGTCGTCTTCGCCCAGACCGATTGAGTTGTGGgt                                                           >  1:3582784/1‑93 (MQ=255)

                              tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgggg                                                           >  1:87502/1‑92 (MQ=255)

                              tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           <  2:5300940/93‑1 (MQ=255)

                              tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           >  2:1556556/1‑93 (MQ=255)

                                tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         >  1:277877/1‑93 (MQ=255)

                                tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         >  1:1315462/1‑93 (MQ=255)

                                tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         >  2:3297451/1‑93 (MQ=255)

                                tAGACATGTTCGGGGTTACGCGCAGAGAAGCATCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         <  1:1515237/93‑1 (MQ=255)

                                     aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    >  2:2839607/1‑93 (MQ=255)

                                     aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    <  1:2273235/93‑1 (MQ=255)

                                      tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   >  2:2372309/1‑93 (MQ=255)

                                      tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCa                                                   >  1:5917037/1‑93 (MQ=255)

                                      tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCCGCGGGTGTAAACCa                                                   >  1:3430100/1‑93 (MQ=255)

                                       gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                                                  <  1:6051742/93‑1 (MQ=255)

                                        ttCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                                                 >  2:2768510/1‑93 (MQ=255)

                                        ttCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGCGAGGCGGGCCGTCTTCGCCCCGACCGATGGAGTCGTGGGTGTAAACCATc                                                 >  2:3463910/1‑93 (MQ=255)

                                          cGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                                               <  2:4023197/93‑1 (MQ=255)

                                          cGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                                               <  2:4794772/93‑1 (MQ=255)

                                            gggTTACGCGCAGAGCAGCGCCCTGCTCCACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             <  2:4772456/93‑1 (MQ=255)

                                             ggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                                            <  1:1842228/93‑1 (MQ=255)

                                              gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTTGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                           <  2:4466219/93‑1 (MQ=255)

                                              gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                           <  1:2771545/93‑1 (MQ=255)

                                                tACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACg                                         >  2:2786598/1‑93 (MQ=255)

                                                 aCGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGc                                        <  2:6044591/93‑1 (MQ=255)

                                                    cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  1:4562165/93‑1 (MQ=255)

                                                      cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  1:4832845/93‑1 (MQ=255)

                                                      cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   >  2:1131794/1‑93 (MQ=255)

                                                      cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  1:3320953/93‑1 (MQ=255)

                                                        gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 >  1:3017235/1‑93 (MQ=255)

                                                        gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 <  1:4698286/93‑1 (MQ=255)

                                                        aagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 <  2:5002470/92‑1 (MQ=255)

                                                          gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               <  1:1042535/93‑1 (MQ=255)

                                                          gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  1:6130940/1‑93 (MQ=255)

                                                          gaAGCGACCTGCTCAACCGGCTGGTGAGTCCGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               <  2:262373/93‑1 (MQ=255)

                                                            aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTTTTTCATCAg                             <  1:4182840/93‑1 (MQ=255)

                                                             gCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                            <  1:1285108/93‑1 (MQ=255)

                                                              cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcg                           <  1:948545/93‑1 (MQ=255)

                                                               gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          >  1:2537282/1‑93 (MQ=255)

                                                                aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                         >  1:2490582/1‑93 (MQ=255)

                                                                  cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGGTGTTTCATCAGCGCGGc                       >  1:2305177/1‑93 (MQ=255)

                                                                  cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       >  2:5136667/1‑93 (MQ=255)

                                                                   tGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGcc                      >  2:4438995/1‑93 (MQ=255)

                                                                   tGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGcc                      >  2:2580/1‑93 (MQ=255)

                                                                     cTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAt                    >  2:749072/1‑93 (MQ=255)

                                                                      tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   <  2:2220151/93‑1 (MQ=255)

                                                                       cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  <  2:4324010/93‑1 (MQ=255)

                                                                         aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgt                <  1:3661280/93‑1 (MQ=255)

                                                                           cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              <  2:3977579/93‑1 (MQ=255)

                                                                           cGGCTGGTGAGTAGGGACGTCTTCGCACAGACCGATAGAGTCGTGGGAGTAAACCATCACCTCACGCTGTTTTATCAGCGCTGCCAtacggac              >  1:4346229/1‑93 (MQ=255)

                                                                            ggCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg             <  1:5065076/93‑1 (MQ=255)

                                                                             gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            <  1:1209489/93‑1 (MQ=255)

                                                                             gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            >  2:5528599/1‑93 (MQ=255)

                                                                             gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            <  1:5402648/93‑1 (MQ=255)

                                                                             gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            >  1:5128003/1‑93 (MQ=255)

                                                                               tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg          >  2:24563/1‑93 (MQ=255)

                                                                               tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg          >  1:5433511/1‑93 (MQ=255)

                                                                               tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg          >  2:3316479/1‑93 (MQ=255)

                                                                                ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:3168521/1‑93 (MQ=255)

                                                                                ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:3804058/1‑93 (MQ=255)

                                                                                ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:1703348/1‑93 (MQ=255)

                                                                                ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  1:255781/1‑93 (MQ=255)

                                                                                 gTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGtt        <  2:3115168/93‑1 (MQ=255)

                                                                                    aGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACg     <  2:5896126/93‑1 (MQ=255)

                                                                                    aGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACg     >  1:628830/1‑93 (MQ=255)

                                                                                     gTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGACGTTACGt    >  1:3459673/1‑93 (MQ=255)

                                                                                      gcGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTg   <  2:1947105/92‑1 (MQ=255)

CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078155‑3078334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTAT  >  NC_000913/3078152‑3078338

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                               >  SRR4302532.2368458/1‑94 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                               >  SRR4302532.4477803/1‑94 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                               <  SRR4302532.6150078/94‑1 (MQ=60)

 ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGC                                                                                              >  SRR4302532.4380140/1‑94 (MQ=60)

 ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGC                                                                                              <  SRR4302532.5865550/94‑1 (MQ=60)

  CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCC                                                                                             >  SRR4302532.4706552/1‑94 (MQ=60)

     CGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTC                                                                                          <  SRR4302532.5223697/94‑1 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                         >  SRR4302532.3224109/1‑94 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                         >  SRR4302532.3224108/1‑94 (MQ=60)

       GATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTT                                                                                        <  SRR4302532.3297610/94‑1 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                       <  SRR4302532.377520/94‑1 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                       <  SRR4302532.857033/94‑1 (MQ=60)

         TTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCG                                                                                      <  SRR4302532.3782752/94‑1 (MQ=60)

         TTCAAACTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCGTCG                                                                                      >  SRR4302532.4423928/1‑94 (MQ=60)

           CAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTATTCGCC                                                                                    >  SRR4302532.2532513/1‑94 (MQ=60)

           CAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCC                                                                                    >  SRR4302532.1802498/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGGCGTCTTCGCCC                                                                                   >  SRR4302532.3741498/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                   >  SRR4302532.4547413/1‑94 (MQ=60)

                TGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAC                                                                               <  SRR4302532.3903961/94‑1 (MQ=60)

                TGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAC                                                                               >  SRR4302532.2260654/1‑94 (MQ=60)

                TGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAC                                                                               <  SRR4302532.3903960/94‑1 (MQ=60)

                 GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                                              <  SRR4302532.4168158/94‑1 (MQ=60)

                 GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                                              <  SRR4302532.1352600/94‑1 (MQ=60)

                   TCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGA                                                                            <  SRR4302532.3982669/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302532.4041657/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302532.2401234/94‑1 (MQ=60)

                     ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                                          <  SRR4302532.2429427/94‑1 (MQ=60)

                     ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCACCAAGTCCGATG                                                                          <  SRR4302532.4430393/94‑1 (MQ=60)

                      CACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGG                                                                         <  SRR4302532.5220625/94‑1 (MQ=60)

                        CGGACTGCATGTAGACATGTTCGGGGTTACTCACAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAG                                                                       <  SRR4302532.1436410/94‑1 (MQ=60)

                        CGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAG                                                                       >  SRR4302532.3407680/1‑94 (MQ=60)

                          GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                                                     <  SRR4302532.4101815/94‑1 (MQ=60)

                          GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                                                     <  SRR4302532.3819691/94‑1 (MQ=60)

                          GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                                                     <  SRR4302532.2274092/94‑1 (MQ=60)

                          GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                                                     <  SRR4302532.3547805/94‑1 (MQ=60)

                             GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                                                  <  SRR4302532.3403495/94‑1 (MQ=60)

                             GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                                                  <  SRR4302532.5795529/94‑1 (MQ=60)

                               CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCCGCACGATGGAGTCGTGGG                                                                >  SRR4302532.4270033/1‑94 (MQ=60)

                               CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                                <  SRR4302532.359416/94‑1 (MQ=60)

                               CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                                >  SRR4302532.1182904/1‑94 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGTCGTCTTCGCCCAGACCGATTGAGTTGTGGGT                                                               >  SRR4302532.3702634/1‑94 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                               >  SRR4302532.3508912/1‑94 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                               <  SRR4302532.829665/94‑1 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGG                                                               >  SRR4302532.90589/1‑94 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGG                                                               >  SRR4302532.2919130/1‑94 (MQ=60)

                                  GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                             >  SRR4302532.1359442/1‑94 (MQ=60)

                                  GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                             >  SRR4302532.6317245/1‑94 (MQ=60)

                                  GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                             >  SRR4302532.287495/1‑94 (MQ=60)

                                   TAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTA                                                            >  SRR4302532.4477379/1‑94 (MQ=60)

                                   TAGACATGTTCGGGGTTACGCGCAGAGAAGCATCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTA                                                            <  SRR4302532.1566409/94‑1 (MQ=60)

                                    AGATATGTTCGGGGTCACGCGCAGAGAAGCCACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                           <  SRR4302532.1793239/94‑1 (MQ=60)

                                    AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGGCGGGCCGTCTTCGCCCAGACCGATGGAGGCGGGGGTGGAA                                                           >  SRR4302532.4932668/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                       <  SRR4302532.2364542/94‑1 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                       >  SRR4302532.3457433/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                       <  SRR4302532.2348665/94‑1 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCA                                                       >  SRR4302532.6121946/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTAGTGGGTGTAAACCA                                                       >  SRR4302532.95383/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCCGCGGGTGTAAACCA                                                       >  SRR4302532.3545035/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGCTGGAGTCGTGGGGGGAAACCA                                                       >  SRR4302532.831011/1‑94 (MQ=60)

                                         TGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAT                                                      <  SRR4302532.4392730/94‑1 (MQ=60)

                                          GTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATC                                                     <  SRR4302532.6260728/94‑1 (MQ=60)

                                          GTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGGGGGTGTAAACCATC                                                     >  SRR4302532.1235928/1‑94 (MQ=60)

                                              GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                                 <  SRR4302532.2699767/94‑1 (MQ=60)

                                               GGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTG                                                <  SRR4302532.286757/94‑1 (MQ=60)

                                               GGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAACCATCACCTG                                                >  SRR4302532.1510807/1‑94 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                               >  SRR4302532.1192890/1‑94 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                               <  SRR4302532.1903417/94‑1 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                               <  SRR4302532.5241609/94‑1 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                               <  SRR4302532.2363043/94‑1 (MQ=60)

                                                GGTTACACGCAGAGAAGCGACCTGGTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCTCAGCCCGATGGAGTCCTGGGTGTCAACCATCACCTGA                                               >  SRR4302532.938036/1‑94 (MQ=60)

                                                 GTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                                              <  SRR4302532.2864602/94‑1 (MQ=60)

                                                  TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                                             >  SRR4302532.452053/1‑94 (MQ=60)

                                                    ACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCT                                           <  SRR4302532.495768/94‑1 (MQ=60)

                                                    ACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCT                                           <  SRR4302532.3266595/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        <  SRR4302532.4717791/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        <  SRR4302532.1171320/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAACTGACGCTGTT                                        >  SRR4302532.1856716/1‑94 (MQ=60)

                                                       CCCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        <  SRR4302532.1339520/94‑1 (MQ=60)

                                                        GCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTT                                       >  SRR4302532.5329008/1‑94 (MQ=60)

                                                        GCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTT                                       <  SRR4302532.5760864/94‑1 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                      <  SRR4302532.3431684/94‑1 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                      <  SRR4302532.4999202/94‑1 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                      >  SRR4302532.4560962/1‑94 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTA                                      >  SRR4302532.5386445/1‑94 (MQ=60)

                                                          AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA                                     >  SRR4302532.3116792/1‑94 (MQ=60)

                                                          AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA                                     <  SRR4302532.6356258/94‑1 (MQ=60)

                                                          AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGACGTGGGTGTAGAACATCACCTGACGatagttct                                     >  SRR4302532.5360939/1‑86 (MQ=60)

                                                           GAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCAT                                    <  SRR4302532.4858545/94‑1 (MQ=60)

                                                           GAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCAT                                    <  SRR4302532.6248720/94‑1 (MQ=60)

                                                            AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                   >  SRR4302532.4749637/1‑94 (MQ=60)

                                                            AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                   >  SRR4302532.6342480/1‑94 (MQ=60)

                                                            AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                   >  SRR4302532.5613411/1‑94 (MQ=60)

                                                             GAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA                                  <  SRR4302532.1077689/94‑1 (MQ=60)

                                                              AAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAG                                 <  SRR4302532.3744099/94‑1 (MQ=60)

                                                               AGCGACCTGCTCAACCGGCTGGTGAGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC                                >  SRR4302532.4438973/1‑94 (MQ=60)

                                                               AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTTTTTCATCAGC                                <  SRR4302532.4323299/94‑1 (MQ=60)

                                                               AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTTTTTCATCAGC                                <  SRR4302532.4323144/94‑1 (MQ=60)

                                                                GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                               <  SRR4302532.1328089/94‑1 (MQ=60)

                                                                 CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC                              >  SRR4302532.2621924/1‑94 (MQ=60)

                                                                 CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC                              >  SRR4302532.3326192/1‑94 (MQ=60)

                                                                 CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC                              <  SRR4302532.980980/94‑1 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                             >  SRR4302532.1551245/1‑94 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                             >  SRR4302532.2573596/1‑94 (MQ=60)

                                                                    CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGGTGTTTCATCAGCGCGGC                           >  SRR4302532.2381822/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302532.1365502/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          <  SRR4302532.3366402/94‑1 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302532.1617667/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302532.3074622/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302532.2602491/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302532.2602492/1‑94 (MQ=60)

                                                                        CTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATA                       >  SRR4302532.989834/1‑94 (MQ=60)

                                                                         TCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATAC                      <  SRR4302532.1310895/94‑1 (MQ=60)

                                                                          CAACGGGCGGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                     <  SRR4302532.2519588/94‑1 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                     >  SRR4302532.3222781/1‑94 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                     >  SRR4302532.5998572/1‑94 (MQ=60)

                                                                            ACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTA                   <  SRR4302532.3784211/94‑1 (MQ=60)

                                                                             CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                  <  SRR4302532.3688474/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACG                 <  SRR4302532.2623648/94‑1 (MQ=60)

                                                                               GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGG                >  SRR4302532.921225/1‑94 (MQ=60)

                                                                               GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGG                >  SRR4302532.5304409/1‑94 (MQ=60)

                                                                               GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGG                <  SRR4302532.5239027/94‑1 (MQ=60)

                                                                                GCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGC               <  SRR4302532.1249956/94‑1 (MQ=60)

                                                                                GCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGC               <  SRR4302532.2259990/94‑1 (MQ=60)

                                                                                GCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGC               <  SRR4302532.5589841/94‑1 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG              >  SRR4302532.5776160/1‑94 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG              >  SRR4302532.2059745/1‑94 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG              >  SRR4302532.5621826/1‑94 (MQ=60)

                                                                                  TGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGT             >  SRR4302532.264663/1‑94 (MQ=60)

                                                                                     TGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTAC          >  SRR4302532.6290469/1‑94 (MQ=60)

                                                                                      GAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACG         >  SRR4302532.650789/1‑94 (MQ=60)

                                                                                       AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGT        <  SRR4302532.618096/94‑1 (MQ=60)

                                                                                       AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGT        <  SRR4302532.2778610/94‑1 (MQ=60)

                                                                                       AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGT        >  SRR4302532.3939230/1‑94 (MQ=60)

                                                                                       AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGACGTTACGT        >  SRR4302532.3575538/1‑94 (MQ=60)

                                                                                          CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG     <  SRR4302532.884952/94‑1 (MQ=60)

                                                                                            GGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTA   >  SRR4302532.1582257/1‑94 (MQ=60)

                                                                                            GGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTA   >  SRR4302532.2878994/1‑94 (MQ=60)

                                                                                            GGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTA   >  SRR4302532.4599595/1‑94 (MQ=60)

                                                                                             GCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATCCGTACGGCGCTACGTGCGTAT  >  SRR4302532.1301042/1‑94 (MQ=60)

                                                                                             GCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTAT  <  SRR4302532.5914226/94‑1 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTAT  >  NC_000913/3078152‑3078338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`