Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,257,838 +G coding (2222/2241 nt) tdcE ← putative formate acetyltransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,257,8371.G100.0% 834.3 / NA 231A741A (GCG→GCC) tdcEputative formate acetyltransferase 3
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (111/120);  total (111/120)

Only 100 of 247 total aligned reads displayed.

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG  >  NC_000913/3257745‑3257924
                                                                                             |                                                                                       
tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                          >  2:5705996/1‑93 (MQ=255)
tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGGAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                          >  1:5948429/1‑93 (MQ=255)
 ttttCATACATCCACCAACGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑g                                                                                        <  2:5499332/93‑1 (MQ=255)
  tttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                       <  2:3096108/93‑2 (MQ=255)
    tCATACATCCTCCGGCGTCAGAGGGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAcagca                                                                                      >  2:518903/1‑89 (MQ=255)
     cATACATCCCCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcgt                                                                                    <  1:4447788/93‑5 (MQ=255)
       tACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAg                                                                                   >  2:2645839/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  2:4173053/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  2:3576724/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  1:775316/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGGACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  1:5591072/1‑93 (MQ=255)
           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                               >  2:3238354/1‑93 (MQ=255)
           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                               >  2:319620/1‑93 (MQ=255)
            cctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCaga                                                                              <  2:5153107/93‑1 (MQ=255)
            cctccGGAGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCaga                                                                              >  1:1562647/1‑93 (MQ=255)
             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             <  2:2148829/93‑1 (MQ=255)
             ctccGGCGTCAGAGCGCCTGGGGAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             >  2:2090639/1‑93 (MQ=255)
              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            <  2:4996170/93‑1 (MQ=255)
              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            >  2:6100136/1‑93 (MQ=255)
              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            >  1:2933775/1‑93 (MQ=255)
              cccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            <  1:1733388/92‑1 (MQ=255)
               ccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGac                                                                           >  2:1064056/1‑93 (MQ=255)
               ccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGac                                                                           <  1:2378417/93‑1 (MQ=255)
               ccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGac                                                                           <  2:2383771/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAATTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  2:5925411/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  1:313066/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  1:3290237/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  1:4335399/93‑1 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                        <  1:546035/93‑1 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                        <  1:6002672/93‑1 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                        <  2:4069292/93‑1 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                      >  1:5422502/1‑93 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                      >  1:1785492/1‑93 (MQ=255)
                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                     >  1:4917123/1‑93 (MQ=255)
                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                     >  2:1007181/1‑93 (MQ=255)
                      cAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAtt                                                                    >  1:2785139/1‑93 (MQ=255)
                       agagCGCCTGGTTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   <  1:587096/93‑1 (MQ=255)
                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   <  2:3679560/93‑1 (MQ=255)
                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   <  2:2713346/93‑1 (MQ=255)
                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   <  1:4536540/93‑1 (MQ=255)
                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGCTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   >  2:5927508/1‑93 (MQ=255)
                        gagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                                                                  >  1:2433694/1‑93 (MQ=255)
                         agCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTc                                                                 <  2:812680/93‑1 (MQ=255)
                           cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                                               >  2:3943288/1‑93 (MQ=255)
                            gcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAgg                                                              >  2:2491497/1‑93 (MQ=255)
                             ccTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGt                                                             >  1:4003869/1‑93 (MQ=255)
                             ccTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGt                                                             <  2:2277153/93‑1 (MQ=255)
                             ccTGGGTAAAGATACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCACACGGCGTAGCCAGAGACACGGATTGTCAGGt                                                             <  1:5006185/93‑1 (MQ=255)
                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                            >  2:2771679/1‑93 (MQ=255)
                                 ggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAgg                                                         >  2:4603729/1‑93 (MQ=255)
                                 ggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAgg                                                         <  1:1367551/93‑1 (MQ=255)
                                   tAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGat                                                       <  2:176591/93‑1 (MQ=255)
                                    aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                      <  1:113246/93‑1 (MQ=255)
                                    aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                      >  2:4200778/1‑93 (MQ=255)
                                     aaGGTACGTGAAATAACACCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGatat                                                     <  1:5070686/93‑1 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                   <  2:509036/93‑1 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                   >  2:3512045/1‑93 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                   <  1:4754932/93‑1 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                   >  1:882038/1‑93 (MQ=255)
                                        gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtttt                                                  >  2:4326092/1‑93 (MQ=255)
                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                 <  2:4742008/93‑1 (MQ=255)
                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                 <  1:5152209/93‑1 (MQ=255)
                                          tagTGAAAAAACATCCTGTTTCTTATCACGGGTGAGTGCGTTGAAGCGAACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc                                                <  2:1651504/91‑1 (MQ=255)
                                          aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc                                                >  1:644555/1‑93 (MQ=255)
                                           cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                               <  1:1496498/93‑1 (MQ=255)
                                            gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                              >  1:2088091/1‑93 (MQ=255)
                                            gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                              <  2:638976/93‑1 (MQ=255)
                                            gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                              >  1:5327071/1‑93 (MQ=255)
                                             tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                             >  1:6145512/1‑93 (MQ=255)
                                              gAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCATAGACACGGATTGTCAGGGTAGGATATTTTTCCggg                                            >  1:2295139/1‑93 (MQ=37)
                                              gAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCggg                                            >  2:5396890/1‑93 (MQ=255)
                                               aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt                                           <  1:2835736/93‑1 (MQ=255)
                                               aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt                                           <  1:2651595/93‑1 (MQ=255)
                                                 aTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc                                         >  1:4234786/1‑93 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        <  2:6041505/93‑1 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        <  2:1309575/93‑1 (MQ=255)
                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                       >  2:436498/1‑93 (MQ=255)
                                                   aaCATCCGGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                       <  2:1492120/93‑1 (MQ=255)
                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     <  1:1226643/93‑1 (MQ=255)
                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     <  2:3393854/93‑1 (MQ=255)
                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     <  2:1958585/93‑1 (MQ=255)
                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     <  1:477510/93‑1 (MQ=255)
                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                   <  1:3433675/93‑1 (MQ=255)
                                                        ccTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATgg                                  <  1:2863800/93‑1 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 <  2:3987705/93‑1 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 >  1:3790569/1‑93 (MQ=255)
                                                            ttGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc                              >  1:848160/1‑93 (MQ=255)
                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTTCGGGTGCTCGATGGCATCcagc                          >  1:256764/1‑93 (MQ=255)
                                                                 gTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                         >  1:4621437/1‑93 (MQ=255)
                                                                     aCGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                     >  2:2181503/1‑93 (MQ=255)
                                                                      cGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                    <  1:1657672/93‑1 (MQ=255)
                                                                         gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                 >  2:1208397/1‑93 (MQ=255)
                                                                               gCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTc           <  2:4572829/93‑1 (MQ=255)
                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         >  2:3868613/1‑93 (MQ=255)
                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        <  2:5513369/93‑1 (MQ=255)
                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        <  2:3409900/93‑1 (MQ=255)
                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  2:1370430/93‑1 (MQ=255)
                                                                                    gAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTac      >  1:4405812/1‑93 (MQ=255)
                                                                                        cgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttg  <  1:2613484/93‑1 (MQ=255)
                                                                                        cgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttg  >  2:3705809/1‑93 (MQ=255)
                                                                                             |                                                                                       
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG  >  NC_000913/3257745‑3257924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT  >  NC_000913/3257747‑3257929
                                                                                            |                                                                                           
TTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggc                                                                                           >  SRR4302532.2823226/1‑92 (MQ=60)
   CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTA                                                                                         >  SRR4302532.5291879/1‑94 (MQ=60)
   CATACATCCCCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTA                                                                                         <  SRR4302532.4599375/94‑1 (MQ=60)
    ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                        <  SRR4302532.1794213/94‑1 (MQ=60)
     TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                       >  SRR4302532.802019/1‑94 (MQ=60)
     TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                       >  SRR4302532.1826219/1‑94 (MQ=60)
     TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGGACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                       >  SRR4302532.5785156/1‑94 (MQ=60)
        ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                    >  SRR4302532.2317722/1‑94 (MQ=60)
         TCCTCCGGAGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGA                                                                                   >  SRR4302532.1615496/1‑94 (MQ=60)
          CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAG                                                                                  <  SRR4302532.5768285/94‑1 (MQ=60)
           CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGA                                                                                 >  SRR4302532.3031285/1‑94 (MQ=60)
            TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGCC                                                                                >  SRR4302532.3174702/1‑94 (MQ=60)
            TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGAC                                                                                <  SRR4302532.2561521/94‑1 (MQ=60)
            CCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGAC                                                                                <  SRR4302532.1791735/94‑1 (MQ=60)
             CCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACA                                                                               <  SRR4302532.2457675/94‑1 (MQ=60)
              CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACAC                                                                              <  SRR4302532.3399695/94‑1 (MQ=60)
              CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACAC                                                                              <  SRR4302532.4481859/94‑1 (MQ=60)
              CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACAC                                                                              <  SRR4302532.6331246/94‑1 (MQ=60)
              CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACAC                                                                              <  SRR4302532.323929/94‑1 (MQ=60)
                GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGG                                                                            <  SRR4302532.565054/94‑1 (MQ=60)
                GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGG                                                                            <  SRR4302532.6209978/94‑1 (MQ=60)
                GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGG                                                                            <  SRR4302532.565055/94‑1 (MQ=60)
                 CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGA                                                                           >  SRR4302532.1845180/1‑94 (MQ=60)
                 CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGA                                                                           >  SRR4302532.5610445/1‑94 (MQ=60)
                  GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAT                                                                          <  SRR4302532.463982/94‑1 (MQ=60)
                  GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAT                                                                          >  SRR4302532.5086041/1‑94 (MQ=60)
                  GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAT                                                                          >  SRR4302532.2995514/1‑94 (MQ=60)
                   TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATT                                                                         >  SRR4302532.2878564/1‑94 (MQ=60)
                     AGAGCGCCTGGTTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGT                                                                       <  SRR4302532.607574/94‑1 (MQ=60)
                     AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGT                                                                       >  SRR4302532.2465821/1‑94 (MQ=60)
                     AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGT                                                                       >  SRR4302532.2514590/1‑94 (MQ=60)
                     AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGT                                                                       >  SRR4302532.902689/1‑94 (MQ=60)
                     AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGT                                                                       <  SRR4302532.4691391/94‑1 (MQ=60)
                      GAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTC                                                                      <  SRR4302532.4799400/94‑1 (MQ=60)
                      GAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTC                                                                      <  SRR4302532.1801029/94‑1 (MQ=60)
                       AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCA                                                                     >  SRR4302532.2931300/1‑94 (MQ=60)
                       AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCA                                                                     >  SRR4302532.3277967/1‑94 (MQ=60)
                         CGCCTGGGTAGAGGTACGTGAAATAGCATCTTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                   <  SRR4302532.5608505/94‑1 (MQ=60)
                         CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                   <  SRR4302532.2664841/94‑1 (MQ=60)
                          GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                  >  SRR4302532.4138750/1‑94 (MQ=60)
                          GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                  >  SRR4302532.4570093/1‑94 (MQ=60)
                           CCTGGGTAAAGATACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCACACGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                 <  SRR4302532.5177761/94‑1 (MQ=60)
                             TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCAGGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                               <  SRR4302532.2777651/94‑1 (MQ=60)
                             TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                               <  SRR4302532.4141082/94‑1 (MQ=60)
                             TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                               <  SRR4302532.2710391/94‑1 (MQ=60)
                               GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                             <  SRR4302532.1413354/94‑1 (MQ=60)
                                GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                            <  SRR4302532.5861946/94‑1 (MQ=60)
                                GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                            >  SRR4302532.1628462/1‑94 (MQ=60)
                                GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                            >  SRR4302532.519507/1‑94 (MQ=60)
                                GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                            <  SRR4302532.6131958/94‑1 (MQ=60)
                                GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                            >  SRR4302532.5322086/1‑94 (MQ=60)
                                 TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                           >  SRR4302532.4816567/1‑94 (MQ=60)
                                  AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                          <  SRR4302532.117134/94‑1 (MQ=60)
                                  AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                          <  SRR4302532.4943544/94‑1 (MQ=60)
                                  AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTACGATAT                                                          >  SRR4302532.5593700/1‑94 (MQ=60)
                                   AAGGTACGTGAAATAACACCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                         <  SRR4302532.5244961/94‑1 (MQ=60)
                                    AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                        >  SRR4302532.343612/1‑94 (MQ=60)
                                    AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                        >  SRR4302532.912194/1‑94 (MQ=60)
                                    AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                        <  SRR4302532.243870/94‑1 (MQ=60)
                                     GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                       <  SRR4302532.3912603/94‑1 (MQ=60)
                                     GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                       <  SRR4302532.4937988/94‑1 (MQ=60)
                                     GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                       <  SRR4302532.4917562/94‑1 (MQ=60)
                                       TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                     <  SRR4302532.5329454/94‑1 (MQ=60)
                                       TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                     >  SRR4302532.2919279/1‑94 (MQ=60)
                                       TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                     >  SRR4302532.666941/1‑94 (MQ=60)
                                        ACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                    <  SRR4302532.1289646/94‑1 (MQ=60)
                                         CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                   >  SRR4302532.5511731/1‑94 (MQ=60)
                                         CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                   <  SRR4302532.1546975/94‑1 (MQ=60)
                                         CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                   >  SRR4302532.2157721/1‑94 (MQ=60)
                                         AGTGAAATAACATCCTGTTGCTGTTCACGGGTGAGTGCGTTGAAGCGGACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAAATTTTTCCG                                                   <  SRR4302532.1546976/94‑1 (MQ=60)
                                          GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                  >  SRR4302532.6357483/1‑94 (MQ=60)
                                           TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCATAGACACGGATTGTCAGGGTAGGATATTTTTCCGGG                                                 >  SRR4302532.2371341/1‑94 (MQ=60)
                                           TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                 >  SRR4302532.709930/1‑94 (MQ=60)
                                             AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                               <  SRR4302532.2930539/94‑1 (MQ=60)
                                             AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                               <  SRR4302532.2740574/94‑1 (MQ=60)
                                              AATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGC                                              >  SRR4302532.4376984/1‑94 (MQ=60)
                                               ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                             >  SRR4302532.802364/1‑94 (MQ=60)
                                                 AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                           <  SRR4302532.227163/94‑1 (MQ=60)
                                                   CATCCTTTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                         <  SRR4302532.1757040/94‑1 (MQ=60)
                                                   CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                         <  SRR4302532.1267680/94‑1 (MQ=60)
                                                   CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                         <  SRR4302532.494162/94‑1 (MQ=60)
                                                   CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGACTGTCAGGTTAGGATATTATTCCGGGTGCTCGAT                                         >  SRR4302532.5689535/1‑94 (MQ=60)
                                                     TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                       <  SRR4302532.2979984/94‑1 (MQ=60)
                                                     TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                       <  SRR4302532.3548725/94‑1 (MQ=60)
                                                     TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                       <  SRR4302532.4478087/94‑1 (MQ=60)
                                                      CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                      <  SRR4302532.2959575/94‑1 (MQ=60)
                                                      CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                      >  SRR4302532.3918452/1‑94 (MQ=60)
                                                      CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                      >  SRR4302532.3745949/1‑94 (MQ=60)
                                                        TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                    >  SRR4302532.5512871/1‑94 (MQ=60)
                                                        TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                    >  SRR4302532.4554096/1‑94 (MQ=60)
                                                         GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                   >  SRR4302532.877322/1‑94 (MQ=60)
                                                         GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                   >  SRR4302532.877320/1‑94 (MQ=60)
                                                           TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                 >  SRR4302532.1503775/1‑94 (MQ=60)
                                                            TCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                <  SRR4302532.1251738/94‑1 (MQ=60)
                                                            GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTATGATATTTTTCCGGGTGCTCGATGGCATCCAG                                <  SRR4302532.2672710/94‑1 (MQ=60)
                                                            GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                >  SRR4302532.772997/1‑94 (MQ=60)
                                                            GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                <  SRR4302532.4771761/94‑1 (MQ=60)
                                                             CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTTCGGGTGCTCGATGGCATCCAGC                               >  SRR4302532.265673/1‑94 (MQ=60)
                                                             CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                               >  SRR4302532.4540559/1‑94 (MQ=60)
                                                              TGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                              <  SRR4302532.5323376/94‑1 (MQ=60)
                                                              TGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                              >  SRR4302532.4779023/1‑94 (MQ=60)
                                                               GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAG                             <  SRR4302532.6206850/94‑1 (MQ=60)
                                                                TTCACGGGGCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACGCGGATTGTCGGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                            <  SRR4302532.3354110/94‑1 (MQ=60)
                                                                TTCACGCGTCAGTGCGTGGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGTCATCCAGCAGC                            <  SRR4302532.3354111/94‑1 (MQ=60)
                                                                    CGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                        <  SRR4302532.1713850/94‑1 (MQ=60)
                                                                    CGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                        <  SRR4302532.6074483/94‑1 (MQ=60)
                                                                    CGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                        >  SRR4302532.4359484/1‑94 (MQ=60)
                                                                     GGGTCAGTGTGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                       <  SRR4302532.169767/94‑1 (MQ=60)
                                                                     GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                       >  SRR4302532.937808/1‑94 (MQ=60)
                                                                     GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                       <  SRR4302532.2497544/94‑1 (MQ=60)
                                                                          AGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                  >  SRR4302532.4039806/1‑94 (MQ=60)
                                                                          AGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                  >  SRR4302532.1457388/1‑94 (MQ=60)
                                                                          AGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                  <  SRR4302532.5459050/94‑1 (MQ=60)
                                                                              CGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT              >  SRR4302532.5032811/1‑94 (MQ=60)
                                                                               GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT             >  SRR4302532.5131833/1‑94 (MQ=60)
                                                                               GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATGTCCCGATTCATT             >  SRR4302532.627407/1‑94 (MQ=60)
                                                                                TTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA            <  SRR4302532.1883734/94‑1 (MQ=60)
                                                                                TTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA            <  SRR4302532.4660141/94‑1 (MQ=60)
                                                                                 TGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC           >  SRR4302532.4555870/1‑94 (MQ=60)
                                                                                  GAAGCGCACGGGGTAGCCAGAGACACGGATTGGCAGGTTAGGATATTTTTCCGGGTGCTCGGTGGGATCCAGCAGCATTTCCCTATTCATTACT          >  SRR4302532.620324/1‑94 (MQ=60)
                                                                                      CGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA      <  SRR4302532.2700916/94‑1 (MQ=60)
                                                                                       GCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC     <  SRR4302532.5961950/94‑1 (MQ=60)
                                                                                       GCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC     <  SRR4302532.6105514/94‑1 (MQ=60)
                                                                                        CACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG    <  SRR4302532.991272/94‑1 (MQ=60)
                                                                                        CACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG    <  SRR4302532.5659498/94‑1 (MQ=60)
                                                                                         acgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGT   >  SRR4302532.175205/4‑94 (MQ=60)
                                                                                         acgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGT   >  SRR4302532.1801318/4‑94 (MQ=60)
                                                                                         acgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGT   >  SRR4302532.5422025/4‑94 (MQ=60)
                                                                                         acgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCACGATTCATTACGTTGACGT   >  SRR4302532.685112/4‑94 (MQ=60)
                                                                                          cgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT  >  SRR4302532.1885550/3‑94 (MQ=60)
                                                                                            |                                                                                           
TTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT  >  NC_000913/3257747‑3257929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑