Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,509,958 Δ1 bp intergenic (‑195/+314) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,509,9560T.100.0% 966.5 / NA 205intergenic (‑193/+316)yhfZ/trpShypothetical protein/tryptophan tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (70/135);  total (70/135)

Only 100 of 224 total aligned reads displayed.

GGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACA  >  NC_000913/3509865‑3510043
                                                                                           |                                                                                       
 gCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttc                                                                                       >  2:1662866/1‑92 (MQ=255)
  cattcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttct                                                                                      <  2:5554419/93‑3 (MQ=255)
   cttcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATTACGACGCcttctt                                                                                     <  2:1915748/92‑4 (MQ=255)
   attcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttctt                                                                                     >  1:1474767/1‑90 (MQ=255)
    ttcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttcttt                                                                                    <  2:2296531/93‑5 (MQ=255)
    ttcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttcttt                                                                                    <  1:738682/93‑5 (MQ=255)
     tcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttt                                                                                  >  1:4409355/1‑93 (MQ=255)
     tcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttt                                                                                  <  2:206282/93‑1 (MQ=255)
     tcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttt                                                                                  <  2:2563031/93‑1 (MQ=255)
       attTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttttt                                                                                >  2:2092829/1‑93 (MQ=255)
       attTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttttt                                                                                <  2:3128382/93‑1 (MQ=255)
         ttaccAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGa                                                                              <  1:3027744/90‑1 (MQ=255)
         ttGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGa                                                                              <  2:75686/93‑1 (MQ=255)
          tGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGAt                                                                             <  2:2289119/93‑1 (MQ=255)
           gCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATa                                                                            <  1:3776314/93‑1 (MQ=255)
           gCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATa                                                                            <  1:2834948/93‑1 (MQ=255)
             cAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATaaa                                                                          <  2:1827806/93‑1 (MQ=255)
              cgCTCATCTATTGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAAc                                                                         <  1:1235346/92‑1 (MQ=255)
               gCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACg                                                                        <  2:1529916/93‑1 (MQ=255)
                cTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGt                                                                       <  2:5974476/93‑1 (MQ=255)
                 tCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGtt                                                                      <  1:2295031/93‑1 (MQ=255)
                 tCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGCCGTGGTGAAGACGCC‑TTCTTTTTTGTTAAACGtt                                                                      >  2:1241602/1‑93 (MQ=255)
                  cATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTc                                                                     <  1:4224292/93‑1 (MQ=255)
                  cATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTc                                                                     <  2:571336/93‑1 (MQ=255)
                      tATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACg                                                                 <  2:528030/93‑1 (MQ=255)
                       aTGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGc                                                                >  2:3563104/1‑93 (MQ=255)
                        tGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCa                                                               <  1:3339527/93‑1 (MQ=255)
                         ggTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCat                                                              <  2:1445566/93‑1 (MQ=255)
                          gTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCata                                                             <  1:964570/93‑1 (MQ=255)
                          gTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGAGAAGACGACGCC‑TGCTTTTATGATAAACGGTCGACGCata                                                             >  2:5467149/1‑93 (MQ=255)
                           ttttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataa                                                            <  2:1388256/93‑1 (MQ=255)
                            tttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataat                                                           >  1:4561108/1‑93 (MQ=255)
                            tttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataat                                                           <  1:3116511/93‑1 (MQ=255)
                             ttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataata                                                          <  2:2706272/93‑1 (MQ=255)
                              tCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCAtaataa                                                         >  1:476170/1‑93 (MQ=255)
                                 aTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAAtt                                                      <  1:4892372/93‑1 (MQ=255)
                                  tCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAAttt                                                     >  2:5209729/1‑93 (MQ=255)
                                  tCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAAttt                                                     <  1:5875120/93‑1 (MQ=255)
                                    gATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTc                                                   <  2:2916924/93‑1 (MQ=255)
                                    gATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTc                                                   <  1:1882540/93‑1 (MQ=255)
                                    gATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTc                                                   <  2:4478116/93‑1 (MQ=255)
                                     aTTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCa                                                  >  2:526017/1‑93 (MQ=255)
                                     aTTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCa                                                  <  1:89591/93‑1 (MQ=255)
                                     aTTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCa                                                  <  2:711392/93‑1 (MQ=255)
                                      ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAg                                                 >  1:3373798/1‑93 (MQ=255)
                                       tACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGt                                                >  2:3414001/1‑93 (MQ=255)
                                       tACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGt                                                <  1:6048190/93‑1 (MQ=255)
                                        tccGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTa                                               <  2:2172723/92‑1 (MQ=255)
                                        aCCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTa                                               <  1:3232859/93‑1 (MQ=255)
                                           gCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGGCGCATAATAAATTTTCAGTAAAt                                            >  1:3483999/1‑93 (MQ=255)
                                           gCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAt                                            <  1:2073592/93‑1 (MQ=255)
                                           gCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAt                                            <  1:3796631/93‑1 (MQ=255)
                                            cACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAtt                                           <  2:5127395/93‑1 (MQ=255)
                                              cTTTTCACCCAACAAATAACGGGACAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGa                                         <  2:4841722/93‑1 (MQ=255)
                                               ttttCGCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGaa                                        <  1:1268233/93‑1 (MQ=255)
                                               ttttCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGaa                                        >  2:2969456/1‑93 (MQ=255)
                                               ttttCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGaa                                        <  1:477768/93‑1 (MQ=255)
                                                 ttCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAata                                      >  2:2854796/1‑93 (MQ=255)
                                                   cACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAtt                                    <  1:1308992/93‑1 (MQ=255)
                                                    aCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAttt                                   <  1:6074721/93‑1 (MQ=255)
                                                    aCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAttt                                   >  1:5092354/1‑93 (MQ=255)
                                                    aCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAttt                                   >  1:3946140/1‑93 (MQ=255)
                                                     cccAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTta                                  <  2:795432/93‑1 (MQ=255)
                                                     cccAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTta                                  <  2:5051662/93‑1 (MQ=255)
                                                       caacaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtata                                <  1:3712178/93‑1 (MQ=255)
                                                       caacaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtata                                <  2:5382398/93‑1 (MQ=255)
                                                       caacaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtata                                <  1:803978/93‑1 (MQ=255)
                                                       caacaaAAAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtata                                <  1:2392178/93‑1 (MQ=255)
                                                        aacaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTatat                               <  1:660473/93‑1 (MQ=255)
                                                        aacaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTatat                               <  1:1700594/93‑1 (MQ=255)
                                                         acaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATc                              <  1:3997646/93‑1 (MQ=255)
                                                         acaaATAACGGGACAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATc                              <  2:1898014/93‑1 (MQ=255)
                                                          caaATACCGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCt                             <  1:2974596/93‑1 (MQ=255)
                                                          caaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCt                             >  1:1528754/1‑93 (MQ=255)
                                                           aaaTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCtt                            >  1:1705158/1‑93 (MQ=255)
                                                            aaTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTc                           <  2:3746739/93‑1 (MQ=255)
                                                            aaTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTc                           <  2:5569566/93‑1 (MQ=255)
                                                             aTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa                          >  1:2700869/1‑93 (MQ=255)
                                                             aTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa                          <  1:4461834/93‑1 (MQ=255)
                                                              tAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTTATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTTAg                         >  1:2429566/1‑93 (MQ=255)
                                                                    gCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAAttt                   <  1:3294122/93‑1 (MQ=255)
                                                                     ccAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTg                  <  1:1031187/93‑1 (MQ=255)
                                                                      cAGCGTCGTGATGACGACGCC‑TTCTTTTATTATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGa                 <  1:1005619/93‑1 (MQ=255)
                                                                       aGCGTCGTGGTGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGaa                >  1:5842459/1‑93 (MQ=255)
                                                                       aGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGaa                >  1:561596/1‑93 (MQ=255)
                                                                       aGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTAAATATTTATATTTTCAGGAATTTgca                >  2:1249844/1‑91 (MQ=255)
                                                                        gCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAg               >  2:3796069/1‑93 (MQ=255)
                                                                        gCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACACATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAg               >  2:5665738/1‑93 (MQ=255)
                                                                           tcgtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATa            >  1:2505514/1‑93 (MQ=255)
                                                                           tcgtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCATCAGGAATTTGAAGATa            >  1:215031/1‑93 (MQ=255)
                                                                            cgtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATaa           >  1:3577092/1‑93 (MQ=255)
                                                                            cgtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATaa           <  2:3645777/93‑1 (MQ=255)
                                                                             gtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCAT‑ATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAgg         <  2:4740558/93‑1 (MQ=255)
                                                                              tgatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAgg         <  1:4067951/93‑1 (MQ=255)
                                                                              tgatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAgg         <  1:5558211/93‑1 (MQ=255)
                                                                               gatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAggg        >  2:4928233/1‑92 (MQ=255)
                                                                               gatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgc        <  1:4936057/93‑1 (MQ=255)
                                                                               gatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgc        <  1:5350462/93‑1 (MQ=255)
                                                                                 tgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgcgc      >  1:5436070/1‑93 (MQ=255)
                                                                                     gacgCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACa  >  2:4324849/1‑93 (MQ=255)
                                                                                           |                                                                                       
GGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACA  >  NC_000913/3509865‑3510043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATG  >  NC_000913/3509865‑3510051
                                                                                              |                                                                                             
GGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCCTTC                                                                                               <  SRR4302532.241879/94‑1 (MQ=60)
  CATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTT                                                                                            >  SRR4302532.1524456/1‑94 (MQ=60)
   ATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTT                                                                                           >  SRR4302532.3669242/1‑94 (MQ=60)
   ATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTT                                                                                           >  SRR4302532.4939642/1‑94 (MQ=60)
    TTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTT                                                                                          <  SRR4302532.763852/94‑1 (MQ=60)
    TTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTT                                                                                          >  SRR4302532.4559557/1‑94 (MQ=60)
     TCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTT                                                                                         <  SRR4302532.878586/94‑1 (MQ=60)
         TTACCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGAT                                                                                     <  SRR4302532.3127651/94‑1 (MQ=60)
          TGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGCGATGACGA‑CGCC‑TTCTTTTTTTATA                                                                                    >  SRR4302532.2732580/1‑94 (MQ=60)
           GCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAA                                                                                   <  SRR4302532.2929725/94‑1 (MQ=60)
           GCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAA                                                                                   <  SRR4302532.3903487/94‑1 (MQ=60)
            CCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAA                                                                                  <  SRR4302532.2570871/94‑1 (MQ=60)
            CCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAA                                                                                  <  SRR4302532.5020884/94‑1 (MQ=60)
             CAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAAC                                                                                 >  SRR4302532.4872848/1‑94 (MQ=60)
              CGCTCATCTATTGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACG                                                                                <  SRR4302532.1276665/94‑1 (MQ=60)
                CTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTT                                                                              <  SRR4302532.697293/94‑1 (MQ=60)
                CTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTT                                                                              >  SRR4302532.2991907/1‑94 (MQ=60)
                 TCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTC                                                                             <  SRR4302532.2371231/94‑1 (MQ=60)
                 TCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTC                                                                             <  SRR4302532.2895855/94‑1 (MQ=60)
                 TCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTA                                                                             >  SRR4302532.285664/1‑94 (MQ=60)
                  CATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCG                                                                            <  SRR4302532.3732800/94‑1 (MQ=60)
                  CATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCG                                                                            <  SRR4302532.4366068/94‑1 (MQ=60)
                  CATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCG                                                                            <  SRR4302532.5440785/94‑1 (MQ=60)
                    TCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGAC                                                                          >  SRR4302532.1855107/1‑94 (MQ=60)
                    TCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGAC                                                                          >  SRR4302532.4939072/1‑94 (MQ=60)
                     CTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACG                                                                         <  SRR4302532.6048613/94‑1 (MQ=60)
                       ATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCA                                                                       <  SRR4302532.1975732/94‑1 (MQ=60)
                       ATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCA                                                                       >  SRR4302532.5917837/1‑94 (MQ=60)
                       ATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGACGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCA                                                                       >  SRR4302532.4547506/1‑94 (MQ=60)
                        TGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCAT                                                                      <  SRR4302532.3450972/94‑1 (MQ=60)
                         GGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATA                                                                     >  SRR4302532.3471314/1‑94 (MQ=60)
                         GGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATA                                                                     >  SRR4302532.4422018/1‑94 (MQ=60)
                          GTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAA                                                                    <  SRR4302532.331619/94‑1 (MQ=60)
                          GTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAA                                                                    <  SRR4302532.997483/94‑1 (MQ=60)
                          GTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAA                                                                    <  SRR4302532.2451185/94‑1 (MQ=60)
                           TTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAAT                                                                   >  SRR4302532.4716707/1‑94 (MQ=60)
                            TTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATA                                                                  <  SRR4302532.4269608/94‑1 (MQ=60)
                            TTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATA                                                                  <  SRR4302532.3219914/94‑1 (MQ=60)
                             TTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAA                                                                 >  SRR4302532.492768/1‑94 (MQ=60)
                              TCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAA                                                                <  SRR4302532.4010807/94‑1 (MQ=60)
                               CAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAAT                                                               >  SRR4302532.6338736/1‑94 (MQ=60)
                                AATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATT                                                              <  SRR4302532.933221/94‑1 (MQ=60)
                                 ATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTT                                                             <  SRR4302532.5060493/94‑1 (MQ=60)
                                 ATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGGCGTGATGGCGACCGCCTTTTTTTTTT‑‑TTAACGTTCGACGCACAATTAATTT                                                             >  SRR4302532.4585633/1‑94 (MQ=50)
                                  TCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTT                                                            <  SRR4302532.6078677/94‑1 (MQ=60)
                                    GATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCA                                                          <  SRR4302532.6258796/94‑1 (MQ=60)
                                    GATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCA                                                          <  SRR4302532.1944957/94‑1 (MQ=60)
                                     ATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAG                                                         <  SRR4302532.92735/94‑1 (MQ=60)
                                     ATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAG                                                         >  SRR4302532.3486627/1‑94 (MQ=60)
                                     ATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAG                                                         >  SRR4302532.3486628/1‑94 (MQ=60)
                                       TACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTA                                                       <  SRR4302532.6257032/94‑1 (MQ=60)
                                        TCCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAA                                                      <  SRR4302532.1550386/94‑1 (MQ=60)
                                        ACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAA                                                      <  SRR4302532.3340159/94‑1 (MQ=60)
                                         CCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAA                                                     <  SRR4302532.3377380/94‑1 (MQ=60)
                                          CGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGGCGCATAATAAATTTTCAGTAAAT                                                    >  SRR4302532.3600703/1‑94 (MQ=60)
                                          CGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAT                                                    <  SRR4302532.1483413/94‑1 (MQ=60)
                                          CGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAT                                                    >  SRR4302532.5257486/1‑94 (MQ=60)
                                           GCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATT                                                   <  SRR4302532.2142698/94‑1 (MQ=60)
                                           GCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATT                                                   <  SRR4302532.3924755/94‑1 (MQ=60)
                                            CACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTG                                                  >  SRR4302532.2195866/1‑94 (MQ=60)
                                             CCTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGA                                                 <  SRR4302532.3117417/94‑1 (MQ=60)
                                             CCTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGA                                                 <  SRR4302532.5585200/94‑1 (MQ=60)
                                               TTTTCGCCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAAT                                               <  SRR4302532.1310658/94‑1 (MQ=60)
                                               TTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAAT                                               <  SRR4302532.494428/94‑1 (MQ=60)
                                                 TTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAT                                             <  SRR4302532.5917751/94‑1 (MQ=60)
                                                   CACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTT                                           >  SRR4302532.5267467/1‑94 (MQ=60)
                                                   CACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTT                                           >  SRR4302532.4079119/1‑94 (MQ=60)
                                                   CACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTT                                           <  SRR4302532.1352800/94‑1 (MQ=60)
                                                   CACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTT                                           <  SRR4302532.4159652/94‑1 (MQ=60)
                                                    ACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTA                                          <  SRR4302532.6284508/94‑1 (MQ=60)
                                                    ACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTA                                          <  SRR4302532.4096016/94‑1 (MQ=60)
                                                     CCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTAT                                         <  SRR4302532.3180753/94‑1 (MQ=60)
                                                      CCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATA                                        <  SRR4302532.1759070/94‑1 (MQ=60)
                                                      CCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATA                                        >  SRR4302532.5453609/1‑94 (MQ=60)
                                                       CAACAAATACCGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATAT                                       <  SRR4302532.5781919/94‑1 (MQ=60)
                                                       CAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATAT                                       <  SRR4302532.831431/94‑1 (MQ=60)
                                                       CAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATAT                                       <  SRR4302532.1261750/94‑1 (MQ=60)
                                                       CAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATAT                                       <  SRR4302532.3836779/94‑1 (MQ=60)
                                                       CAACAAAAAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATAT                                       <  SRR4302532.2471868/94‑1 (MQ=60)
                                                        AACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATC                                      <  SRR4302532.683312/94‑1 (MQ=60)
                                                        AACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATC                                      <  SRR4302532.1757959/94‑1 (MQ=60)
                                                         ACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCT                                     <  SRR4302532.4132326/94‑1 (MQ=60)
                                                         ACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCT                                     >  SRR4302532.1580394/1‑94 (MQ=60)
                                                          CAAATACCGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTT                                    <  SRR4302532.3073021/94‑1 (MQ=60)
                                                          CAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTT                                    <  SRR4302532.1348554/94‑1 (MQ=60)
                                                          CAAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTT                                    >  SRR4302532.1762654/1‑94 (MQ=60)
                                                           AAATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTC                                   <  SRR4302532.1459775/94‑1 (MQ=60)
                                                            AATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCA                                  >  SRR4302532.2791594/1‑94 (MQ=60)
                                                             ATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTTATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTTAG                                 >  SRR4302532.2510319/1‑94 (MQ=60)
                                                             ATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAG                                 <  SRR4302532.4650008/94‑1 (MQ=60)
                                                             ATAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAG                                 <  SRR4302532.4613931/94‑1 (MQ=60)
                                                              AAACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGG                                <  SRR4302532.2365335/94‑1 (MQ=60)
                                                                ACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAA                              <  SRR4302532.1647383/94‑1 (MQ=60)
                                                                ACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAA                              <  SRR4302532.6125994/94‑1 (MQ=60)
                                                                ACGGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAA                              >  SRR4302532.3415915/1‑94 (MQ=60)
                                                                  GGGCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATT                            >  SRR4302532.1045446/1‑94 (MQ=60)
                                                                    GCCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTG                          <  SRR4302532.3403735/94‑1 (MQ=60)
                                                                     CCAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGA                         <  SRR4302532.1066022/94‑1 (MQ=60)
                                                                      CAGCGTCGTGGTGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAA                        >  SRR4302532.6045232/1‑94 (MQ=60)
                                                                      CAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAA                        >  SRR4302532.3956419/1‑94 (MQ=60)
                                                                      CAGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAA                        >  SRR4302532.581166/1‑94 (MQ=60)
                                                                      CAGCGTCGTGATGACGA‑CGCC‑TTCTTTTATTATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAA                        <  SRR4302532.1039838/94‑1 (MQ=60)
                                                                       AGCGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTTAGGAATTTGAAG                       <  SRR4302532.6332780/94‑1 (MQ=60)
                                                                         GGTCGTGATGACGT‑CGCC‑TTCTTTTTTGATAAACGTTCCACGCATAATCAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGAT                     >  SRR4302532.4906603/1‑94 (MQ=60)
                                                                         CGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGAT                     <  SRR4302532.813795/94‑1 (MQ=60)
                                                                         CGTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGAT                     <  SRR4302532.563302/94‑1 (MQ=60)
                                                                          GTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATA                    >  SRR4302532.2589083/1‑94 (MQ=60)
                                                                          GTCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCATCAGGAATTTGAAGATA                    >  SRR4302532.222364/1‑94 (MQ=60)
                                                                           TCGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAA                   >  SRR4302532.3696777/1‑94 (MQ=60)
                                                                            CGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAG                  <  SRR4302532.3540730/94‑1 (MQ=60)
                                                                            CGTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAG                  >  SRR4302532.3271924/1‑94 (MQ=60)
                                                                             GTGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGG                 >  SRR4302532.5085965/1‑94 (MQ=60)
                                                                              TGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGC                <  SRR4302532.5751194/94‑1 (MQ=60)
                                                                              TGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGC                <  SRR4302532.4204597/94‑1 (MQ=60)
                                                                              TGATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGC                <  SRR4302532.1880117/94‑1 (MQ=60)
                                                                               GATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCG               <  SRR4302532.5105527/94‑1 (MQ=60)
                                                                               GATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCG               <  SRR4302532.5536061/94‑1 (MQ=60)
                                                                                ATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGC              >  SRR4302532.3867036/1‑94 (MQ=60)
                                                                                ATGACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGC              >  SRR4302532.5624469/1‑94 (MQ=60)
                                                                                   ACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAAC           <  SRR4302532.3919430/94‑1 (MQ=60)
                                                                                   ACGA‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAAC           >  SRR4302532.2491987/1‑94 (MQ=60)
                                                                                      A‑CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGC        >  SRR4302532.2950606/1‑94 (MQ=60)
                                                                                        gacccTTCTTTTTTGATAACCGATCGACGCATAATAAATTGTCAGTAAATTGAATATTTAAATCGTCAGGAAGTTGAAGATAAGGCCCAACAGC        <  SRR4302532.4293601/89‑1 (MQ=60)
                                                                                        CGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCG       <  SRR4302532.5454955/94‑1 (MQ=60)
                                                                                           CCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAG     >  SRR4302532.4611221/1‑94 (MQ=60)
                                                                                            CTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGGATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGA    >  SRR4302532.4425529/1‑94 (MQ=60)
                                                                                             TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAT   <  SRR4302532.3166916/94‑1 (MQ=60)
                                                                                             TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAT   >  SRR4302532.2412242/1‑94 (MQ=60)
                                                                                              TCTTTTTTGATAAACGTTCGACGTATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATG  <  SRR4302532.3605037/94‑1 (MQ=60)
                                                                                              TCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATG  >  SRR4302532.3825615/1‑94 (MQ=60)
                                                                                              |                                                                                             
GGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGA‑CGCCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATG  >  NC_000913/3509865‑3510051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑