Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,944,6760CG100.0% 782.9 / NA 217intergenic (+20/+76)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (98/119);  total (98/119)
*NC_0009133,944,6770GC100.0% 799.9 / NA 217intergenic (+21/+75)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (98/119);  total (98/119)

Only 100 of 219 total aligned reads displayed.

AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763
                                                                                       ||                                                                                      
 gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAgg                                                                                   >  1:4394977/1‑93 (MQ=255)
 gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAgg                                                                                   >  1:2249153/1‑93 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  >  2:2058986/1‑93 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 >  2:3407379/1‑93 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 >  2:103768/1‑93 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 >  2:190942/1‑93 (MQ=255)
     aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttt                                                                               <  2:4604017/93‑1 (MQ=255)
     aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttt                                                                               >  1:1510716/1‑93 (MQ=255)
      aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGACATCATCCTTAGAGAAAGCTAAGGGtttt                                                                              >  2:1796122/1‑93 (MQ=255)
          gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttttt                                                                          <  1:1115739/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  2:2314230/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:917635/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:705017/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  2:868276/93‑1 (MQ=255)
              agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCt                                                                      >  1:2799969/1‑93 (MQ=255)
                 aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaa                                                                   >  2:2257401/1‑93 (MQ=255)
                 aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaa                                                                   <  1:3828504/93‑1 (MQ=255)
                  ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaaa                                                                  >  2:921737/1‑93 (MQ=255)
                   cGGTCTCCAAAACCGGGTGTTGGGAGTTAGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                 >  2:774413/1‑93 (MQ=255)
                      tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                              <  2:3247644/93‑1 (MQ=255)
                       ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                             <  2:6069948/93‑1 (MQ=255)
                       ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                             <  1:837001/93‑1 (MQ=255)
                       ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                             <  1:1242995/93‑1 (MQ=255)
                       ctcCAAAACCGGGTGTTGGGAGGTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                             >  1:1852218/1‑93 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            <  2:4172324/93‑1 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            <  1:5837485/93‑1 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            <  1:4160008/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           >  1:3010132/1‑93 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  2:143463/93‑1 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          <  1:5042116/93‑1 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         <  1:5638017/93‑1 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        <  2:1618185/93‑1 (MQ=255)
                             aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTcc                                                       <  1:1011310/93‑1 (MQ=255)
                                cGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                    >  2:4502994/1‑93 (MQ=255)
                                 gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                   >  2:302085/1‑93 (MQ=255)
                                  ggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGt                                                  >  2:5351004/1‑93 (MQ=255)
                                    tgtTGGGAGTTTAAGGCTCCCCGCCCCTTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaa                                                <  1:3261166/93‑1 (MQ=255)
                                      ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                              <  1:3898706/93‑1 (MQ=255)
                                      gtGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                              <  1:2110443/92‑1 (MQ=255)
                                       tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                             >  2:5218198/1‑93 (MQ=255)
                                       tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                             <  2:2016869/93‑1 (MQ=255)
                                       tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                             <  2:2391983/93‑1 (MQ=255)
                                       tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                             <  2:1117537/93‑1 (MQ=255)
                                        gggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATcc                                            >  2:5915269/1‑93 (MQ=255)
                                           aGTTCGAGTCTATCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTc                                         <  2:6135468/93‑1 (MQ=255)
                                           aGTTCGAGTCGCTCGCCCCCTGCCAGAAATCATCCGTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGGAAATCCTTc                                         <  2:456863/93‑1 (MQ=255)
                                             ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                       >  1:2039331/1‑93 (MQ=255)
                                             ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                       >  2:163248/1‑93 (MQ=255)
                                              tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAc                                      <  1:5529312/93‑1 (MQ=255)
                                                   tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                 >  2:4271997/1‑93 (MQ=255)
                                                   tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                 <  2:1767346/93‑1 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                               <  1:4710326/93‑1 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                               >  1:329441/1‑93 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                               <  2:5684574/93‑1 (MQ=255)
                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                              <  2:1011968/93‑1 (MQ=255)
                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                              <  2:5316487/93‑1 (MQ=255)
                                                      cgccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                              <  2:3557046/91‑1 (MQ=255)
                                                       tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                             <  2:1986440/93‑1 (MQ=255)
                                                          gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc                          >  1:1828736/1‑93 (MQ=255)
                                                          gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc                          <  2:2428789/93‑1 (MQ=255)
                                                           ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                         <  2:1190288/93‑1 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  1:5375671/1‑93 (MQ=255)
                                                             ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                       >  2:3318339/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      >  1:133815/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      <  1:3868631/93‑1 (MQ=255)
                                                               tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcc                     >  2:5811863/1‑93 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    >  1:3336066/1‑93 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    <  2:3101493/93‑1 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    >  2:5100029/1‑93 (MQ=255)
                                                                 ccAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAt                   <  1:3112815/93‑1 (MQ=255)
                                                                 ccAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAt                   >  1:3555567/1‑93 (MQ=255)
                                                                  cAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                  >  1:1977947/1‑93 (MQ=255)
                                                                   aGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTc                 >  2:577281/1‑93 (MQ=255)
                                                                   aGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTc                 <  1:6023658/93‑1 (MQ=255)
                                                                    gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                <  2:2697085/93‑1 (MQ=255)
                                                                     aaaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCat               <  1:4937617/93‑1 (MQ=255)
                                                                     aaaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCat               <  2:4787812/93‑1 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              >  1:5520035/1‑93 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             <  1:2275422/93‑1 (MQ=255)
                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt            >  1:1311192/1‑93 (MQ=255)
                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt            <  1:162587/93‑1 (MQ=255)
                                                                         catcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTa           >  1:933847/1‑93 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          <  1:2845777/93‑1 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:4865035/1‑93 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:3919998/1‑93 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:1169389/1‑93 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         <  1:4831933/93‑1 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         >  1:1596141/1‑93 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         <  2:2111914/93‑1 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         <  1:1141276/93‑1 (MQ=255)
                                                                            ccTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATa        <  2:3462884/93‑1 (MQ=255)
                                                                            ccTGAGCGAAAGCTAAGTATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTCCCGGCATCCTTGCCAGCCATTCATATTAATa        <  1:2492075/93‑1 (MQ=255)
                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac       <  2:5193340/93‑1 (MQ=255)
                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac       >  1:3940497/1‑93 (MQ=255)
                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac       >  2:2521037/1‑93 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      >  2:3145558/1‑93 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      >  1:3247911/1‑93 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      >  2:712729/1‑93 (MQ=255)
                                                                               tAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac     <  1:2903989/93‑1 (MQ=255)
                                                                                  cGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc  <  1:775362/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  >  NC_000913/3944583‑3944764
                                                                                             |                                                                                        
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAG                                                                                          <  SRR4302532.5595180/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAG                                                                                          >  SRR4302532.5414104/1‑94 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAG                                                                                          >  SRR4302532.6202786/1‑94 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                     <  SRR4302532.4076263/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                     <  SRR4302532.6072935/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                     >  SRR4302532.5272818/1‑94 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                    >  SRR4302532.4544480/1‑94 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                    <  SRR4302532.1728665/94‑1 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                    <  SRR4302532.5412618/94‑1 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                    >  SRR4302532.2323824/1‑94 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                    >  SRR4302532.2898520/1‑94 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                  >  SRR4302532.3451056/1‑94 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                  <  SRR4302532.3379415/94‑1 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                >  SRR4302532.1561728/1‑94 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                >  SRR4302532.1561729/1‑94 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                <  SRR4302532.968314/94‑1 (MQ=60)
              TGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTT                                                                            >  SRR4302532.2479835/1‑94 (MQ=60)
                GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTT                                                                          >  SRR4302532.3267779/1‑94 (MQ=60)
                GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTA                                                                          <  SRR4302532.1153017/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                         <  SRR4302532.729120/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                         <  SRR4302532.949033/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                         <  SRR4302532.894191/94‑1 (MQ=60)
                  AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATC                                                                        >  SRR4302532.6159926/1‑94 (MQ=60)
                   GAGCCCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                       <  SRR4302532.567217/94‑1 (MQ=60)
                   GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                       >  SRR4302532.2893794/1‑94 (MQ=60)
                    AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTG                                                                      >  SRR4302532.5722921/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                    <  SRR4302532.5353492/94‑1 (MQ=60)
                       gccgaTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCGTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                   <  SRR4302532.4508322/89‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                   <  SRR4302532.3957763/94‑1 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                  >  SRR4302532.3681651/1‑94 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                 <  SRR4302532.3207482/94‑1 (MQ=60)
                          GGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAA                                                                >  SRR4302532.3217874/1‑94 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                               <  SRR4302532.4879741/94‑1 (MQ=60)
                            TCTCCAAAACCGGGTGTTGGGAGGTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                              >  SRR4302532.1913671/1‑94 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                             <  SRR4302532.1284585/94‑1 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                             <  SRR4302532.865711/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                            <  SRR4302532.6040103/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                            <  SRR4302532.4299537/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                            <  SRR4302532.4605766/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                            >  SRR4302532.3109509/1‑94 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                           <  SRR4302532.199728/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                           <  SRR4302532.2944906/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                          <  SRR4302532.5215078/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                          >  SRR4302532.337399/1‑94 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                          <  SRR4302532.4089084/94‑1 (MQ=60)
                                 AAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTC                                                         <  SRR4302532.5833914/94‑1 (MQ=60)
                                   actgcggtgtttggatgtcgagctcctcctgCCCTGCCAGTAATCATCCTTAGTTAAAGCTAAGGATTTTTTTTATCTGAAATATCCCTCTCCG                                                       <  SRR4302532.4069917/63‑1 (MQ=30)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                       <  SRR4302532.1045683/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                       <  SRR4302532.5111160/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                       <  SRR4302532.2758751/94‑1 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                    <  SRR4302532.3973175/94‑1 (MQ=60)
                                        GGGGTTGGGAGTTCGAGGCTCTCCGCCCCTGCCAGGAATCATCCTTAGCGGAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCTAAGTA                                                  >  SRR4302532.146278/1‑94 (MQ=60)
                                          TGTTGGGAGTTTAAGGCTCCCCGCCCCTTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                <  SRR4302532.3369585/94‑1 (MQ=60)
                                          TGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                >  SRR4302532.2344515/1‑94 (MQ=60)
                                          TGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                >  SRR4302532.1204916/1‑94 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                              <  SRR4302532.4467180/94‑1 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                              <  SRR4302532.4030209/94‑1 (MQ=60)
                                            GTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                              <  SRR4302532.2180884/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                            <  SRR4302532.2176430/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                            <  SRR4302532.5896526/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                            >  SRR4302532.4003636/1‑94 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                        >  SRR4302532.2107266/1‑94 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                        >  SRR4302532.4038686/1‑94 (MQ=60)
                                                   TTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAC                                       <  SRR4302532.1151922/94‑1 (MQ=60)
                                                    TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACG                                      >  SRR4302532.1352377/1‑94 (MQ=60)
                                                    TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACC                                      <  SRR4302532.5721198/94‑1 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                     >  SRR4302532.2421684/1‑94 (MQ=60)
                                                      GAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGG                                    <  SRR4302532.652890/94‑1 (MQ=60)
                                                        GTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCA                                  <  SRR4302532.3074177/94‑1 (MQ=60)
                                                         TCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCATCTCCGAAGTAAATCCTTCTACCGCCAT                                 >  SRR4302532.4973599/1‑94 (MQ=60)
                                                          CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                >  SRR4302532.340874/1‑94 (MQ=60)
                                                           TCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCC                               <  SRR4302532.4871060/94‑1 (MQ=60)
                                                             cccaCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCACTCCGAAGTAAATCCTTCTACCGGCATCCTT                             <  SRR4302532.2214533/90‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                             <  SRR4302532.700285/94‑1 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                            <  SRR4302532.1160914/94‑1 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                           >  SRR4302532.5067185/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                           >  SRR4302532.1889600/1‑94 (MQ=60)
                                                                GCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                          <  SRR4302532.3702111/94‑1 (MQ=60)
                                                                CCCCCTGGCAGAAATCGTCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                          <  SRR4302532.1902755/94‑1 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                         >  SRR4302532.5561900/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                        >  SRR4302532.832306/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTCGCCAG                        >  SRR4302532.156680/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                       >  SRR4302532.138323/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGCCATCCTTGACAGC                       >  SRR4302532.2065486/1‑94 (MQ=60)
                                                                    CTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCC                      <  SRR4302532.3412288/94‑1 (MQ=60)
                                                                    CTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCC                      <  SRR4302532.3999182/94‑1 (MQ=60)
                                                                     TGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                     >  SRR4302532.3447372/1‑94 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                    >  SRR4302532.3674536/1‑94 (MQ=60)
                                                                       CCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                   >  SRR4302532.2043642/1‑94 (MQ=60)
                                                                       CCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                   <  SRR4302532.3216090/94‑1 (MQ=60)
                                                                         AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                 <  SRR4302532.6231689/94‑1 (MQ=60)
                                                                           AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA               >  SRR4302532.5711591/1‑94 (MQ=60)
                                                                           AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA               <  SRR4302532.5107129/94‑1 (MQ=60)
                                                                           AAATCATCCCTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA               >  SRR4302532.5711592/1‑94 (MQ=60)
                                                                             ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATT             <  SRR4302532.2350934/94‑1 (MQ=60)
                                                                             ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATT             >  SRR4302532.1355063/1‑94 (MQ=60)
                                                                              TCATCCTTAGCGAAAGCTAAGTATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA            <  SRR4302532.168007/94‑1 (MQ=60)
                                                                              TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA            <  SRR4302532.168008/94‑1 (MQ=60)
                                                                              TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA            >  SRR4302532.965845/1‑94 (MQ=60)
                                                                                ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT          <  SRR4302532.2940896/94‑1 (MQ=60)
                                                                                ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT          >  SRR4302532.1650180/1‑94 (MQ=60)
                                                                                 TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA         <  SRR4302532.4998254/94‑1 (MQ=60)
                                                                                 TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA         <  SRR4302532.1179331/94‑1 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC        >  SRR4302532.4073285/1‑94 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC        <  SRR4302532.753179/94‑1 (MQ=60)
                                                                                  CCTGAGCGAAAGCTAAGTATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTCCCGGCATCCTTGCCAGCCATTCATATTAATAC        <  SRR4302532.2575152/94‑1 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA       >  SRR4302532.964710/1‑94 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA       >  SRR4302532.3355745/1‑94 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA       >  SRR4302532.1375736/1‑94 (MQ=60)
                                                                                    TTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACAC      <  SRR4302532.1943512/94‑1 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT     <  SRR4302532.3000872/94‑1 (MQ=60)
                                                                                      AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT    >  SRR4302532.4069490/1‑94 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  <  SRR4302532.802065/94‑1 (MQ=60)
                                                                                             |                                                                                        
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  >  NC_000913/3944583‑3944764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑