Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,960,578 2 bp→CG coding (1266‑1267/1485 nt) gppA ← guanosine pentaphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,960,5780GC100.0% 899.5 / NA 251L423V (CTC→GTC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (105/146);  total (105/146)
*NC_0009133,960,5790CG100.0% 901.9 / NA 251R422R (CGG→CGC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (105/146);  total (105/146)

Only 100 of 263 total aligned reads displayed.

TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960490‑3960665
                                                                                        ||                                                                                      
  tAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcgg                                                                                   >  2:3985521/1‑93 (MQ=255)
   aaTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggg                                                                                  >  2:586625/1‑92 (MQ=255)
   aaTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc                                                                                  >  1:668800/1‑93 (MQ=255)
   aaTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc                                                                                  >  1:1854028/1‑93 (MQ=255)
    aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 >  1:1483104/1‑93 (MQ=255)
     tGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGcggcgg                                                                                >  2:448882/1‑93 (MQ=255)
      gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               >  2:6133772/1‑93 (MQ=255)
       tCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa                                                                              <  1:1060343/93‑1 (MQ=255)
       tCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa                                                                              >  1:5755363/1‑93 (MQ=255)
       tCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa                                                                              >  1:4124324/1‑93 (MQ=255)
        cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGTCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGTCGCGCGGCGGTAc                                                                             >  1:2444460/1‑93 (MQ=255)
        cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             <  1:3496374/93‑1 (MQ=255)
        cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             >  1:1956017/1‑93 (MQ=255)
         aTCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACg                                                                            <  1:4856478/93‑1 (MQ=255)
          tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           <  1:4920872/93‑1 (MQ=255)
          tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           <  2:5158191/93‑1 (MQ=255)
            tctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa                                                                         >  2:3288462/1‑93 (MQ=255)
            tctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa                                                                         <  1:325137/93‑1 (MQ=255)
              tGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtt                                                                       <  2:3641749/93‑1 (MQ=255)
              tGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGAT‑GCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAttt                                                                      <  1:3876995/93‑1 (MQ=255)
                  aCGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAAGGGCAGAGTTGTTCTGCGACGCGCGGCGGTCCGGCAttttct                                                                   >  1:5134353/1‑91 (MQ=255)
                    gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg                                                                 >  2:137652/1‑93 (MQ=255)
                     agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  1:1092084/93‑1 (MQ=255)
                     agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  2:3005063/93‑1 (MQ=255)
                     agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                >  2:3829092/1‑93 (MQ=255)
                       aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCCGGGGTAGTAAACGGCAGAGCTGTTCTGCGACGCGCGGCGGTACGGCATTTTGTTGATg                                                              >  1:864320/1‑93 (MQ=255)
                        tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGc                                                             <  1:3305612/93‑1 (MQ=255)
                             aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:1226216/93‑1 (MQ=255)
                             aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  1:5978643/93‑1 (MQ=255)
                             aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  1:580939/93‑1 (MQ=255)
                             aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:3178025/93‑1 (MQ=255)
                             aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:4287502/93‑1 (MQ=255)
                               ggCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATg                                                      >  2:1435683/1‑93 (MQ=255)
                                gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  2:1039625/93‑1 (MQ=255)
                                gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     >  1:2114689/1‑93 (MQ=255)
                                gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  1:3022951/93‑1 (MQ=255)
                                  gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgaga                                                   <  2:6143064/93‑1 (MQ=255)
                                   aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag                                                  >  1:2727581/1‑93 (MQ=255)
                                    cGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 <  2:2423833/93‑1 (MQ=255)
                                    cGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 <  1:2772714/93‑1 (MQ=255)
                                     ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                >  2:6458/1‑93 (MQ=255)
                                     ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                <  2:4590623/93‑1 (MQ=255)
                                      gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  2:3133956/93‑1 (MQ=255)
                                      gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  2:342143/93‑1 (MQ=255)
                                      gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  2:3958643/93‑1 (MQ=255)
                                      gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  2:605431/93‑1 (MQ=255)
                                      gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               >  1:3737383/1‑93 (MQ=255)
                                      gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  1:952827/93‑1 (MQ=255)
                                       cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg                                              <  1:5726112/93‑1 (MQ=255)
                                        tGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCgc                                             >  1:1663040/1‑92 (MQ=255)
                                         ggCAAATATGATAGCAAAGAGTAGTAAACGGCAGAGTTGTTCAGCGACGCGCGGCGGTACGGCATTTAGCTGATGCAGCGATGAGAAATCAAc                                            <  1:5492733/93‑1 (MQ=255)
                                         ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc                                            <  2:706178/93‑1 (MQ=255)
                                         ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc                                            >  1:4218445/1‑93 (MQ=255)
                                          gCAAAAATGATGGCCAGGCGTATTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc                                           >  2:1178767/1‑93 (MQ=255)
                                          gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc                                           <  2:5673537/93‑1 (MQ=255)
                                          gCAAAAATGATGGCCAGGCGTAGTAAACGGAAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc                                           <  1:36953/93‑1 (MQ=255)
                                           cAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCg                                          <  2:1116938/93‑1 (MQ=255)
                                           cAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCg                                          <  1:2513298/93‑1 (MQ=255)
                                            aaaaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg                                         >  1:2924951/1‑93 (MQ=255)
                                            aaaaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg                                         >  1:1770179/1‑93 (MQ=255)
                                             aaaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGa                                        >  2:390860/1‑93 (MQ=255)
                                              aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAt                                       >  2:5290194/1‑93 (MQ=255)
                                              aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAt                                       <  2:348585/93‑1 (MQ=255)
                                                atgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTa                                     <  1:4796670/93‑1 (MQ=255)
                                                  gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt                                   <  1:5339455/93‑1 (MQ=255)
                                                   atgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc                                  <  2:842386/93‑1 (MQ=255)
                                                   atgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc                                  <  2:2800766/93‑1 (MQ=255)
                                                    tgGCCAGGCGTATTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCt                                 <  1:4689747/93‑1 (MQ=255)
                                                     ggCCGGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTg                                <  2:1875528/93‑1 (MQ=255)
                                                     ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTg                                <  2:4124324/93‑1 (MQ=255)
                                                       ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGt                              <  1:2570576/93‑1 (MQ=255)
                                                         aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg                            >  2:2569313/1‑93 (MQ=255)
                                                         aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg                            >  2:1706958/1‑93 (MQ=255)
                                                          ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           >  2:3924941/1‑93 (MQ=255)
                                                           gCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAg                          >  1:2169436/1‑93 (MQ=255)
                                                             gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        <  1:4071513/93‑1 (MQ=255)
                                                             gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        >  2:2494716/1‑93 (MQ=255)
                                                             gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        >  2:5089151/1‑93 (MQ=255)
                                                             gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        <  2:2040846/93‑1 (MQ=255)
                                                               agtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGTGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      >  2:405387/1‑93 (MQ=255)
                                                               agtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      <  2:4588084/93‑1 (MQ=255)
                                                                gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa                     >  1:5868692/1‑93 (MQ=255)
                                                                 taAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAg                    >  1:3876652/1‑93 (MQ=255)
                                                                  aaaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTATGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGc                   <  2:2679666/93‑1 (MQ=255)
                                                                    aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 >  2:5555082/1‑93 (MQ=255)
                                                                    aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 <  2:173054/93‑1 (MQ=255)
                                                                     cGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTc                <  2:544921/93‑1 (MQ=255)
                                                                     cGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTc                <  1:1343849/93‑1 (MQ=255)
                                                                      ggCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCg               >  1:1262321/1‑93 (MQ=255)
                                                                       gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              >  2:520633/1‑93 (MQ=255)
                                                                       gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              >  1:3786066/1‑93 (MQ=255)
                                                                       gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              >  2:4909044/1‑93 (MQ=255)
                                                                       gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              <  1:3135757/93‑1 (MQ=255)
                                                                        cAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGcc             >  2:3685083/1‑93 (MQ=255)
                                                                          gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAg           <  2:2270947/93‑1 (MQ=255)
                                                                          gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAg           <  1:2707004/93‑1 (MQ=255)
                                                                             ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        >  1:904366/1‑93 (MQ=255)
                                                                               gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      <  1:6057838/93‑1 (MQ=255)
                                                                               gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      >  1:5309328/1‑93 (MQ=255)
                                                                                ttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttt     <  1:1410411/93‑1 (MQ=255)
                                                                                        ||                                                                                      
TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960490‑3960665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           <  SRR4302532.2316343/94‑1 (MQ=60)
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           <  SRR4302532.5026108/94‑1 (MQ=60)
   CCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGA‑‑CGCG                                                                                      >  SRR4302532.5575864/1‑94 (MQ=60)
   CCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGA‑‑CGCG                                                                                      >  SRR4302532.142047/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      >  SRR4302532.4616133/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302532.6117485/94‑1 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302532.4226697/94‑1 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      >  SRR4302532.5572211/1‑94 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGG                                                                                     <  SRR4302532.1242607/94‑1 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGG                                                                                     >  SRR4302532.500018/1‑94 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGG                                                                                     <  SRR4302532.2056450/94‑1 (MQ=60)
       TAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGC                                                                                    >  SRR4302532.691876/1‑94 (MQ=60)
       TAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGC                                                                                    >  SRR4302532.1915533/1‑94 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   >  SRR4302532.1533109/1‑94 (MQ=60)
        AATGCCATCTCTGGCACGAGATCGTCACGGCGACGGGTGGCAAAAATGATGGCCAGGCGTAGGAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   >  SRR4302532.1381366/1‑94 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302532.1815115/1‑94 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302532.3910566/1‑94 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302532.3910567/1‑94 (MQ=60)
          TGTCATCTCTGGCGCGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCTTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 <  SRR4302532.1893944/94‑1 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 >  SRR4302532.1937879/1‑94 (MQ=60)
           GTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTC                                                                                >  SRR4302532.3136462/1‑94 (MQ=60)
           GTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTA                                                                                >  SRR4302532.4262731/1‑94 (MQ=60)
           GTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTA                                                                                >  SRR4302532.5955677/1‑94 (MQ=60)
           GTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTA                                                                                >  SRR4302532.5967137/1‑94 (MQ=60)
            TCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGTCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGTCGCGCGGCGGTAC                                                                               >  SRR4302532.2525740/1‑94 (MQ=60)
            TCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAC                                                                               >  SRR4302532.2020990/1‑94 (MQ=60)
            TCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAC                                                                               <  SRR4302532.1096059/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302532.3613513/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              >  SRR4302532.3099772/1‑94 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              >  SRR4302532.1186375/1‑94 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             >  SRR4302532.5020174/1‑94 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             <  SRR4302532.5023578/94‑1 (MQ=60)
               TCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGC                                                                            <  SRR4302532.5089904/94‑1 (MQ=60)
                CTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCA                                                                           >  SRR4302532.5145249/1‑94 (MQ=60)
                 TCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAT                                                                          <  SRR4302532.336412/94‑1 (MQ=60)
                  CTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATT                                                                         >  SRR4302532.2844459/1‑94 (MQ=60)
                   TGTCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        <  SRR4302532.2132749/94‑1 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGAT‑GCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTT                                                                       <  SRR4302532.4007861/94‑1 (MQ=60)
                     GCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTG                                                                      <  SRR4302532.2907631/94‑1 (MQ=60)
                     GCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTG                                                                      <  SRR4302532.2805374/94‑1 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAAGGGCAGAGTTGTTCTGCGACGCGCGGCGGTCCGGCATTTTct                                                                     >  SRR4302532.5310970/1‑92 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGC                                                                     >  SRR4302532.794010/1‑94 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    <  SRR4302532.2343894/94‑1 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    >  SRR4302532.6021427/1‑94 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302532.65265/94‑1 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302532.1128618/94‑1 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302532.237309/94‑1 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302532.5429215/94‑1 (MQ=60)
                           GATCGTCACGGCGACGGCTGGCAAAAATGATGGCCCGGGGTAGTAAACGGCAGAGCTGTTCTGCGACGCGCGGCGGTACGGCATTTTGTTGATG                                                                >  SRR4302532.893964/1‑94 (MQ=60)
                           GATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATG                                                                >  SRR4302532.3390256/1‑94 (MQ=60)
                             TCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCA                                                              <  SRR4302532.3415681/94‑1 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            <  SRR4302532.932519/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302532.1003859/94‑1 (MQ=60)
                                  ACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAT                                                         <  SRR4302532.6185262/94‑1 (MQ=60)
                                  ACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAT                                                         <  SRR4302532.601197/94‑1 (MQ=60)
                                  ACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAT                                                         <  SRR4302532.601196/94‑1 (MQ=60)
                                    GGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGA                                                       >  SRR4302532.2185342/1‑94 (MQ=60)
                                     GCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302532.3122660/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    >  SRR4302532.2819293/1‑94 (MQ=60)
                                       GACGGCTGGCAAAAATGATAGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCTGAGAGGAGAG                                                    >  SRR4302532.1277360/1‑94 (MQ=60)
                                        ACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGA                                                   <  SRR4302532.3633327/94‑1 (MQ=60)
                                        ACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGA                                                   <  SRR4302532.4183811/94‑1 (MQ=60)
                                        ACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGA                                                   <  SRR4302532.896127/94‑1 (MQ=60)
                                        ACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGA                                                   <  SRR4302532.1679853/94‑1 (MQ=60)
                                        ACGGCTGGAAAAAATGATGGCCAGGCGTACTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGA                                                   <  SRR4302532.5840424/94‑1 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  <  SRR4302532.2865810/94‑1 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  >  SRR4302532.3757313/1‑94 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  >  SRR4302532.1873334/1‑94 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTACTAAACGGCAGGTTTGTTCTGCGACGCGCGGCGGTCCGGCATTTTGATGATGCAGCGATGAGAGAT                                                  <  SRR4302532.2385461/94‑1 (MQ=60)
                                         AGGCTGCCAAAAATGGTGGCCAGGCCTAATAAACGGCAGAGTTTTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  <  SRR4302532.3262036/94‑1 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302532.3415718/94‑1 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 >  SRR4302532.3862958/1‑94 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302532.6002111/94‑1 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302532.985386/94‑1 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302532.281101/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGC                                               >  SRR4302532.1719376/1‑94 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               <  SRR4302532.5925540/94‑1 (MQ=60)
                                             TGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAC                                              >  SRR4302532.4360035/1‑94 (MQ=60)
                                              ggcaaatatgatagcaaagaGTAGTAAACGGCAGAGTTGTTCAGCGACGCGCGGCGGTACGGCATTTAGCTGATGCAGCGATGAGAAATCAACC                                             <  SRR4302532.5683203/74‑1 (MQ=58)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             >  SRR4302532.4040156/1‑94 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            <  SRR4302532.4324593/94‑1 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            <  SRR4302532.38315/94‑1 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGAAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            <  SRR4302532.38314/94‑1 (MQ=60)
                                                CAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGG                                           <  SRR4302532.2597150/94‑1 (MQ=60)
                                                CAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGG                                           >  SRR4302532.3022293/1‑94 (MQ=60)
                                                CAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGG                                           <  SRR4302532.3147381/94‑1 (MQ=60)
                                                CAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGG                                           >  SRR4302532.1829499/1‑94 (MQ=60)
                                                CAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGG                                           <  SRR4302532.2597365/94‑1 (MQ=60)
                                                 AAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGA                                          <  SRR4302532.255580/94‑1 (MQ=60)
                                                    AATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTA                                       <  SRR4302532.2773578/94‑1 (MQ=60)
                                                     ATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAG                                      <  SRR4302532.4961866/94‑1 (MQ=60)
                                                      TGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGT                                     <  SRR4302532.2541350/94‑1 (MQ=60)
                                                      TGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGT                                     <  SRR4302532.6081554/94‑1 (MQ=60)
                                                       GATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTC                                    <  SRR4302532.5524660/94‑1 (MQ=60)
                                                        ATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCT                                   <  SRR4302532.2728494/94‑1 (MQ=60)
                                                         TGGCCAGGCGTATTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302532.4849697/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302532.4007832/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302532.2935012/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302532.4630652/94‑1 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 <  SRR4302532.4305506/94‑1 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 <  SRR4302532.3981827/94‑1 (MQ=60)
                                                           GCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGT                                <  SRR4302532.4923235/94‑1 (MQ=60)
                                                           GCCAGGCGTAGTAAACGCCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGGTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGT                                <  SRR4302532.59450/94‑1 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               >  SRR4302532.542443/1‑94 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               <  SRR4302532.1462900/94‑1 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               <  SRR4302532.2656402/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            >  SRR4302532.1769441/1‑94 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            >  SRR4302532.2241971/1‑94 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302532.1125806/94‑1 (MQ=60)
                                                                 CGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCA                          <  SRR4302532.1505923/94‑1 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         <  SRR4302532.949550/94‑1 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         >  SRR4302532.5793485/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         <  SRR4302532.4208255/94‑1 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       >  SRR4302532.6072079/1‑94 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      <  SRR4302532.4216623/94‑1 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      >  SRR4302532.4007500/1‑94 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      >  SRR4302532.3250443/1‑94 (MQ=60)
                                                                        AACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGT                   <  SRR4302532.1413139/94‑1 (MQ=60)
                                                                          CGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCG                 >  SRR4302532.1304546/1‑94 (MQ=60)
                                                                          CGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCG                 >  SRR4302532.5053963/1‑94 (MQ=60)
                                                                          CGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCG                 <  SRR4302532.1388779/94‑1 (MQ=60)
                                                                           GGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGGGCAGTAGCGTCGC                >  SRR4302532.1395757/1‑94 (MQ=60)
                                                                           GGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGC                >  SRR4302532.5516916/1‑94 (MQ=60)
                                                                           GGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGC                >  SRR4302532.3913773/1‑94 (MQ=60)
                                                                            GCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCC               <  SRR4302532.3239840/94‑1 (MQ=60)
                                                                             CAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGAGTCGCCA              >  SRR4302532.1329957/1‑94 (MQ=60)
                                                                               GAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGC            <  SRR4302532.2798031/94‑1 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           <  SRR4302532.5621808/94‑1 (MQ=60)
                                                                                 GTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAA          >  SRR4302532.935222/1‑94 (MQ=60)
                                                                                  TTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAT         >  SRR4302532.6242817/1‑94 (MQ=60)
                                                                                   TGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTTGCCAGCAATT        >  SRR4302532.3507256/1‑94 (MQ=60)
                                                                                   TGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATT        >  SRR4302532.5492920/1‑94 (MQ=60)
                                                                                    TTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302532.5061967/94‑1 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302532.6267002/94‑1 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302532.2215773/94‑1 (MQ=60)
                                                                                     TTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTT      <  SRR4302532.1457697/94‑1 (MQ=60)
                                                                                     TTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTT      <  SRR4302532.3938745/94‑1 (MQ=60)
                                                                                      TCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTT     <  SRR4302532.6199138/94‑1 (MQ=60)
                                                                                        TGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTC   <  SRR4302532.826997/94‑1 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302532.995637/94‑1 (MQ=60)
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑