Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG100.0% 776.2 / NA 217R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (104/113);  total (104/113)
*NC_0009133,969,1040GC100.0% 731.6 / NA 207R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (95/112);  total (95/112)

Only 100 of 221 total aligned reads displayed.

TCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC  >  NC_000913/3969011‑3969188
                                                                                            ||                                                                                    
     atCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctgg                                                                                  >  1:430685/1‑93 (MQ=255)
     atCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctgg                                                                                  >  1:241208/1‑93 (MQ=255)
      tcgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc                                                                                 >  1:6040026/1‑93 (MQ=255)
      tcgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc                                                                                 >  1:2460636/1‑93 (MQ=255)
       cgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                >  1:72951/1‑93 (MQ=255)
        gtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg                                                                               >  2:1053554/1‑93 (MQ=255)
         tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                              >  2:2724607/1‑93 (MQ=255)
            aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGGCTGGCTGATTg                                                                           >  1:3339478/1‑93 (MQ=255)
            aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                           >  2:744220/1‑93 (MQ=255)
             aCCTGATTTGGCGAGTGTAGAAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                          <  2:1406867/93‑1 (MQ=255)
               cTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                        <  2:1847303/93‑1 (MQ=255)
               cTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                        <  1:3423422/93‑1 (MQ=255)
                tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                       >  1:5207149/1‑93 (MQ=255)
                   tttGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                    >  2:1466469/1‑93 (MQ=255)
                   tttGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                    >  2:1346120/1‑93 (MQ=255)
                    tgggCGAGTGAAGTAAAACCTGCCGTAGAAGTCGTTTTTTTCCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc                                                                   <  2:3568502/91‑1 (MQ=255)
                     tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                  <  2:3427830/93‑1 (MQ=255)
                      ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTTTACCCAc                                                                 <  2:4184583/93‑1 (MQ=255)
                      ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc                                                                 >  1:4000936/1‑93 (MQ=255)
                      ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc                                                                 >  1:473870/1‑93 (MQ=255)
                         gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                              >  1:499757/1‑93 (MQ=255)
                         gAGTGTAGAAAAAACTGCGGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                              <  1:3677153/93‑1 (MQ=255)
                            tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                           <  1:6028545/93‑1 (MQ=255)
                              gattaAAAGCGGCCGTAGAAGGCGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                         <  2:255953/90‑1 (MQ=255)
                               agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTTAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                        <  1:751233/93‑1 (MQ=255)
                               agaaaaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                        <  1:4767429/90‑1 (MQ=255)
                                gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       <  2:4704667/93‑1 (MQ=255)
                                gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       <  2:5804758/93‑1 (MQ=255)
                                 taAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                      >  2:4856829/1‑93 (MQ=255)
                                   aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                    <  2:846075/93‑1 (MQ=255)
                                   aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                    <  2:944743/93‑1 (MQ=255)
                                    aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                   >  1:1984947/1‑93 (MQ=255)
                                     aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCgt                                                  >  1:2655015/1‑92 (MQ=255)
                                        tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                               <  2:1097385/93‑1 (MQ=255)
                                        tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                               >  2:5722469/1‑93 (MQ=255)
                                        tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                               <  2:5356815/93‑1 (MQ=255)
                                        tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                               <  2:505364/93‑1 (MQ=255)
                                         gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                              <  2:812201/93‑1 (MQ=255)
                                         gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                              <  1:4378303/93‑1 (MQ=255)
                                            gTAGAGGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                           <  1:5521058/93‑1 (MQ=255)
                                            gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                           >  2:4594320/1‑93 (MQ=255)
                                             tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                          <  1:1013265/93‑1 (MQ=255)
                                             tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                          >  2:2371176/1‑93 (MQ=255)
                                              aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc                                         >  1:1433011/1‑93 (MQ=255)
                                              aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc                                         >  2:5868185/1‑93 (MQ=255)
                                              aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc                                         <  1:2713150/93‑1 (MQ=255)
                                               gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                        >  2:3032488/1‑93 (MQ=255)
                                               gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                        >  1:1390894/1‑93 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      <  2:1039822/93‑1 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCAACGCCGTTTAAGGGCGATCATGAGCCAGa                                      >  1:2556406/1‑93 (MQ=255)
                                                  ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                     <  2:2669992/93‑1 (MQ=255)
                                                   gCGTTTTTTTGAGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                    <  2:5400510/93‑1 (MQ=255)
                                                   gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                    <  1:5350338/93‑1 (MQ=255)
                                                   gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                    <  2:5065788/93‑1 (MQ=255)
                                                     ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg                                  <  2:3979715/93‑1 (MQ=255)
                                                     ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg                                  <  1:4571015/93‑1 (MQ=255)
                                                      gTTTTTTAGGAGCGAGCAAGACGCCAGTTGAAGCGGATGCGTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa                                 <  1:688500/93‑1 (MQ=255)
                                                      gTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATgc                                 >  2:5183919/1‑92 (MQ=255)
                                                      gTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa                                 >  1:1063881/1‑93 (MQ=255)
                                                      gTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa                                 >  1:4539070/1‑93 (MQ=255)
                                                       ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCATGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                >  2:5926958/1‑93 (MQ=255)
                                                        tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCATATATGAcc                               >  2:2321785/1‑93 (MQ=255)
                                                        tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCATTTAAGGGCGATCATGAGCCAGATATGAcc                               >  2:1916487/1‑93 (MQ=255)
                                                         ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACACCGTTTAAGGGCGATCATGAGCCAGATATGACCt                              >  1:2674147/1‑93 (MQ=255)
                                                         ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTAATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt                              <  2:3385860/93‑1 (MQ=255)
                                                          tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                             <  2:2320438/93‑1 (MQ=255)
                                                          tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                             >  2:1517139/1‑93 (MQ=255)
                                                          tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                             <  1:5066854/93‑1 (MQ=255)
                                                           ttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc                            <  2:2443760/93‑1 (MQ=255)
                                                            tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                           >  2:1373870/1‑93 (MQ=255)
                                                            tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                           <  1:315947/93‑1 (MQ=255)
                                                            tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                           <  2:1238027/93‑1 (MQ=255)
                                                             aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                          >  2:2288955/1‑93 (MQ=255)
                                                             aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                          <  2:2920320/93‑1 (MQ=255)
                                                                 gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                      >  2:4667309/1‑93 (MQ=255)
                                                                  cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                     <  1:3874500/93‑1 (MQ=255)
                                                                  cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                     <  1:1694321/93‑1 (MQ=255)
                                                                   gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                    <  2:2025271/93‑1 (MQ=255)
                                                                   gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                    <  2:2977290/93‑1 (MQ=255)
                                                                   gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAGATGACCTACGTTGAATc                    >  2:5491962/1‑93 (MQ=255)
                                                                    agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                   <  2:3348611/93‑1 (MQ=255)
                                                                    agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                   >  1:4647627/1‑93 (MQ=255)
                                                                     gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                  <  2:4333369/93‑1 (MQ=255)
                                                                     gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                  <  2:4538845/93‑1 (MQ=255)
                                                                      cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  1:2990479/93‑1 (MQ=255)
                                                                      cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  2:2159035/93‑1 (MQ=255)
                                                                      cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  2:2796644/93‑1 (MQ=255)
                                                                       acgacgCCAGTTGAAGTGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAAGTCCTACGTTGAATCGgct                >  1:5483858/1‑93 (MQ=255)
                                                                       acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                <  1:595439/93‑1 (MQ=255)
                                                                       acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                <  2:3667649/93‑1 (MQ=255)
                                                                         gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgc              <  1:5679748/93‑1 (MQ=255)
                                                                         gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAACCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgc              <  2:3786567/93‑1 (MQ=255)
                                                                             ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc          <  1:4120066/93‑1 (MQ=255)
                                                                              cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATCTGACCTACGTTAAATCGGCTgctcgcc         >  2:2106616/1‑92 (MQ=255)
                                                                              cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgct         >  2:3233572/1‑93 (MQ=255)
                                                                                 ttGAAGCGGATGCCTGGCTTATTTCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCa      <  1:1823117/93‑1 (MQ=255)
                                                                                   gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    <  1:402458/93‑1 (MQ=255)
                                                                                   gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    <  1:1213317/93‑1 (MQ=255)
                                                                                   gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    <  2:3266433/93‑1 (MQ=255)
                                                                                     aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc  >  1:1608479/1‑93 (MQ=255)
                                                                                            ||                                                                                    
TCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC  >  NC_000913/3969011‑3969188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  NC_000913/3969010‑3969189
                                                                                             |                                                                                      
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                        <  SRR4302532.2305423/94‑1 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                        >  SRR4302532.3802475/1‑94 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACAGCCGTAGAAGGCGGTTTTTTACGAGCGAGGCCGCCGCCAGTTGAAGCGGATC                                                                                        >  SRR4302532.4719189/1‑94 (MQ=60)
     TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                   >  SRR4302532.249535/1‑94 (MQ=60)
     TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                   >  SRR4302532.445611/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                  >  SRR4302532.2542504/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                  >  SRR4302532.2542503/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                  >  SRR4302532.6248614/1‑94 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGGCTGGCT                                                                                 >  SRR4302532.75559/1‑94 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                 <  SRR4302532.2208058/94‑1 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                 >  SRR4302532.75558/1‑94 (MQ=60)
        CGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTG                                                                                >  SRR4302532.6248452/1‑94 (MQ=60)
         GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGA                                                                               >  SRR4302532.5251396/1‑94 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                              >  SRR4302532.775446/1‑94 (MQ=60)
           CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                             >  SRR4302532.4356049/1‑94 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGGCTGGCTGATTG                                                                            >  SRR4302532.3450921/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                           >  SRR4302532.2705762/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                           >  SRR4302532.2472325/1‑94 (MQ=60)
              ACGTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                          <  SRR4302532.55410/94‑1 (MQ=60)
              ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                          >  SRR4302532.3908730/1‑94 (MQ=60)
               CCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                         >  SRR4302532.3523969/1‑94 (MQ=60)
               CCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                         <  SRR4302532.1065446/94‑1 (MQ=60)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                        <  SRR4302532.3538108/94‑1 (MQ=60)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                        >  SRR4302532.5386710/1‑94 (MQ=60)
                 tgttgtgtggagcaaaaaagaaccccgggtggaaggggtttttttgggaccgaaCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                       <  SRR4302532.1366999/40‑1 (MQ=54)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                      >  SRR4302532.2509146/1‑94 (MQ=60)
                     tgggggtttgtataaaacgctgtcgttgaaggtgttttttttcgagctgGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCA                                                                   <  SRR4302532.1546969/45‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                  >  SRR4302532.490384/1‑94 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                  >  SRR4302532.490385/1‑94 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                  >  SRR4302532.4135719/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                               >  SRR4302532.517237/1‑94 (MQ=60)
                          GAGTGTAGAAAAAACTGCGGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                              <  SRR4302532.3800688/94‑1 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                             >  SRR4302532.6162193/1‑94 (MQ=60)
                            gtgaaaaaaaaactcgcgaagaagggttTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                            <  SRR4302532.4602964/66‑1 (MQ=60)
                             tgatGTAAAAACTGCTGTAGAAGGCGGTTTTTGACGGTTAAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTCCCCACGCCGTTT                                                           <  SRR4302532.6046263/90‑1 (MQ=46)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                           <  SRR4302532.6236752/94‑1 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                          >  SRR4302532.748830/1‑94 (MQ=60)
                               TAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAA                                                         <  SRR4302532.5711035/94‑1 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTTAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                        <  SRR4302532.776960/94‑1 (MQ=60)
                                AGAAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                        <  SRR4302532.4930960/94‑1 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                    >  SRR4302532.6095520/1‑94 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                    >  SRR4302532.2050881/1‑94 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                    >  SRR4302532.1510023/1‑94 (MQ=60)
                                     aaactgccctaaaagctttttttggACGAGGGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                   <  SRR4302532.4679866/69‑1 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGT                                                   >  SRR4302532.2744119/1‑94 (MQ=60)
                                       ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                 <  SRR4302532.4895302/94‑1 (MQ=60)
                                       ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                 <  SRR4302532.4895303/94‑1 (MQ=60)
                                       ACGGCCGTAGAGGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                 <  SRR4302532.5751889/94‑1 (MQ=60)
                                          gcggaagagggatgttttttcgGAGCGGGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                              <  SRR4302532.1545185/72‑1 (MQ=60)
                                          GCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                              <  SRR4302532.4526985/94‑1 (MQ=60)
                                          GCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                              >  SRR4302532.1271312/1‑94 (MQ=60)
                                           CCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                             >  SRR4302532.4425351/1‑94 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGGGATCATGAG                                            >  SRR4302532.1172191/1‑94 (MQ=60)
                                             GTAGAGGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                           <  SRR4302532.5712646/94‑1 (MQ=60)
                                             GTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                           >  SRR4302532.1697848/1‑94 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                          >  SRR4302532.1481252/1‑94 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                          <  SRR4302532.1047684/94‑1 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                          >  SRR4302532.1481254/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                         >  SRR4302532.422349/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                         >  SRR4302532.1437527/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                         <  SRR4302532.2804405/94‑1 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                       >  SRR4302532.451111/1‑94 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCAACGCCGTTTAAGGGCGATCATGAGCCAGA                                       >  SRR4302532.2641676/1‑94 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGCACGACGCCAGGTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                       >  SRR4302532.1647944/1‑94 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                    <  SRR4302532.5535932/94‑1 (MQ=60)
                                                      GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                  <  SRR4302532.4726921/94‑1 (MQ=60)
                                                      GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                  >  SRR4302532.1099684/1‑94 (MQ=60)
                                                      GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                  >  SRR4302532.4694006/1‑94 (MQ=60)
                                                       ttttatagcgggcgcGCACGACGCCAGTTGAAGCGGGTGCCTGGCTGATTGCTGTCCCCGCGCCGTTTAAGGGCGACCATGAGCCAGACACGAC                                 <  SRR4302532.4631736/79‑1 (MQ=46)
                                                       GTTTTTTAGGAGCGAGCAAGACGCCAGTTGAAGCGGATGCGTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAC                                 <  SRR4302532.712121/94‑1 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                               <  SRR4302532.5230402/94‑1 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACACCGTTTAAGGGCGATCATGAGCCAGATATGACCT                               >  SRR4302532.2763904/1‑94 (MQ=60)
                                                           TTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                             <  SRR4302532.5240910/94‑1 (MQ=60)
                                                             TACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGT                           <  SRR4302532.326915/94‑1 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                        >  SRR4302532.4790474/1‑94 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                        <  SRR4302532.3658307/94‑1 (MQ=60)
                                                                  ggtAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                      <  SRR4302532.2747607/91‑1 (MQ=60)
                                                                   CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                     <  SRR4302532.1751524/94‑1 (MQ=60)
                                                                   CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                     <  SRR4302532.309644/94‑1 (MQ=60)
                                                                   CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                     <  SRR4302532.4005251/94‑1 (MQ=60)
                                                                    GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                    <  SRR4302532.390579/94‑1 (MQ=60)
                                                                    GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                    >  SRR4302532.4806080/1‑94 (MQ=60)
                                                                     AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                   >  SRR4302532.2777778/1‑94 (MQ=60)
                                                                       CACGACGCCAGTTGAAGTGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAAGTCCTACGTTGAATCGGCT                 >  SRR4302532.5673986/1‑94 (MQ=60)
                                                                       CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                 >  SRR4302532.2987574/1‑94 (MQ=60)
                                                                       CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                 <  SRR4302532.3089312/94‑1 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                <  SRR4302532.616231/94‑1 (MQ=60)
                                                                        ACGACGCCAGGTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                <  SRR4302532.616232/94‑1 (MQ=60)
                                                                          GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT              >  SRR4302532.6316130/1‑94 (MQ=60)
                                                                          GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT              <  SRR4302532.5878040/94‑1 (MQ=60)
                                                                           ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC             <  SRR4302532.5528532/94‑1 (MQ=60)
                                                                            CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG            >  SRR4302532.3369563/1‑94 (MQ=60)
                                                                             GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGACTCGGCTGCTCGT           >  SRR4302532.5065404/1‑94 (MQ=60)
                                                                              CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT          <  SRR4302532.4258331/94‑1 (MQ=60)
                                                                              CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT          >  SRR4302532.1912720/1‑94 (MQ=60)
                                                                               CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCTGCTGCTAGCTC         >  SRR4302532.2506453/1‑94 (MQ=60)
                                                                               CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTC         >  SRR4302532.4118000/1‑94 (MQ=60)
                                                                                  TTGAAGCGGATGCCTGGCTTATTTCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAT      <  SRR4302532.1883838/94‑1 (MQ=60)
                                                                                   TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT     <  SRR4302532.186657/94‑1 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG    >  SRR4302532.5443973/1‑94 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG    <  SRR4302532.416510/94‑1 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG    <  SRR4302532.1253923/94‑1 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG    <  SRR4302532.416511/94‑1 (MQ=60)
                                                                                     AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC   <  SRR4302532.2816242/94‑1 (MQ=60)
                                                                                     AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC   >  SRR4302532.1662925/1‑94 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  SRR4302532.231281/1‑94 (MQ=60)
                                                                                             |                                                                                      
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  NC_000913/3969010‑3969189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑