Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,987,586	2 bp→GC	coding (814‑815/963 nt)	hemC ←	porphobilinogen deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,987,586	0	C	G	100.0%	816.5 / NA	231	R272A (CGG→GCG)	hemC	porphobilinogen deaminase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (137/94); total (137/94)
*	NC_000913	3,987,587	0	G	C	100.0%	811.8 / NA	231	R272A (CGG→GCG)	hemC	porphobilinogen deaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (137/94); total (137/94)

Only 100 of 242 total aligned reads displayed.

AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987499‑3987673

||

aGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGgcgcgccg                                                                                    >  1:2258723/1‑93 (MQ=255)

   tcttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAcc                                                                                 >  2:1917277/1‑93 (MQ=255)

    cttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCa                                                                                >  2:4311220/1‑93 (MQ=255)

     ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAcccg                                                                               >  2:2592985/1‑91 (MQ=255)

     ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               <  1:6080177/93‑1 (MQ=255)

     ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               >  1:3440180/1‑93 (MQ=255)

     ctctGCCAGCGAAATCCCCATTGGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               <  1:863903/92‑1 (MQ=255)

       ctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcg                                                                             <  1:5711348/93‑1 (MQ=255)

       ctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcg                                                                             <  2:3197899/93‑1 (MQ=255)

        tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcgc                                                                            >  2:323678/1‑93 (MQ=255)

         gCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgca                                                                           <  2:3626052/93‑1 (MQ=255)

            aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                        >  2:5978084/1‑93 (MQ=255)

            aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                        >  1:2959252/1‑93 (MQ=255)

            aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                        <  1:2844183/93‑1 (MQ=255)

            aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                        >  1:2625144/1‑93 (MQ=255)

             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCTCCGACCAGcgcacgca                                                                       >  2:5492804/1‑93 (MQ=255)

             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       <  1:5892170/93‑1 (MQ=255)

             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       >  1:5760214/1‑93 (MQ=255)

             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       >  2:5351051/1‑93 (MQ=255)

               gAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCCGCGCACGCAGc                                                                     >  1:148109/1‑93 (MQ=255)

                 aaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                   >  2:4793247/1‑93 (MQ=255)

                   tacccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGCCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  1:5026527/3‑93 (MQ=255)

                   tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  2:1314849/1‑93 (MQ=255)

                   tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 <  1:4005515/93‑1 (MQ=255)

                   tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCCGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  2:2891690/1‑93 (MQ=255)

                   tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACAGTCAGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  2:690048/1‑93 (MQ=255)

                    ccccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAt                                                                <  1:1364286/93‑1 (MQ=255)

                     cccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                               >  1:2098509/1‑93 (MQ=255)

                      ccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAttt                                                              >  1:1921013/1‑93 (MQ=255)

                       cATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             <  1:67148/93‑1 (MQ=255)

                        aTTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTcc                                                            >  1:4562415/1‑92 (MQ=255)

                         tttGTTCGGCATCTTGCGGCGAACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           >  2:5178586/1‑93 (MQ=255)

                          ttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGcc                                                          <  2:666519/93‑1 (MQ=255)

                                ggCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    <  1:3568349/93‑1 (MQ=255)

                                 gCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGGCCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  2:3845745/93‑1 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  2:1542846/1‑93 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  1:2771700/1‑93 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  2:4622125/1‑93 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  2:4156009/1‑93 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                <  1:4690274/93‑1 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  1:1803564/1‑93 (MQ=255)

                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  1:4070029/1‑93 (MQ=255)

                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              >  2:1723937/1‑93 (MQ=255)

                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              <  1:1008724/93‑1 (MQ=255)

                                       tGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCg                                             >  2:3483561/1‑93 (MQ=255)

                                         cggcgCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGc                                           <  2:869727/93‑1 (MQ=255)

                                            cgcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTa                                        <  2:4988928/93‑1 (MQ=255)

                                            cgcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTa                                        <  2:3017558/93‑1 (MQ=255)

                                              cACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGc                                      <  1:4418328/93‑1 (MQ=255)

                                               aCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     >  1:2368583/1‑93 (MQ=255)

                                               aCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     >  1:3367945/1‑93 (MQ=255)

                                               aCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     <  2:1610681/93‑1 (MQ=255)

                                                 cgcgGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                   <  1:5002561/93‑1 (MQ=255)

                                                  gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:1483613/1‑93 (MQ=255)

                                                   cggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCa                                 <  1:536942/93‑1 (MQ=255)

                                                   cggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCa                                 >  2:5205750/1‑93 (MQ=255)

                                                    ggggTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCaa                                <  1:3649420/90‑1 (MQ=255)

                                                    ggcgTTCACCGCGAATAATCTGCGAACAGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCaa                                >  1:3947159/1‑93 (MQ=255)

                                                     gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  1:2211962/93‑1 (MQ=255)

                                                     gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  1:4024024/93‑1 (MQ=255)

                                                      ggTTCTCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAtt                              <  1:1083346/92‑1 (MQ=255)

                                                      cgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAtt                              <  1:902968/93‑1 (MQ=255)

                                                       gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCACTTg                             >  2:400604/1‑93 (MQ=255)

                                                       gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             <  1:4392846/93‑1 (MQ=255)

                                                        ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            <  2:784152/93‑1 (MQ=255)

                                                        ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            <  1:1601178/93‑1 (MQ=255)

                                                         tCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           <  1:3230282/93‑1 (MQ=255)

                                                          cACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCa                          <  1:3189444/93‑1 (MQ=255)

                                                          cACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCa                          >  1:4935413/1‑93 (MQ=255)

                                                           aCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAc                         >  2:6149147/1‑93 (MQ=255)

                                                           aCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAc                         >  1:260170/1‑93 (MQ=255)

                                                           aCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGAAGCTACCAATTGGCAc                         >  2:1918362/1‑93 (MQ=255)

                                                             cgcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCt                       >  1:5270746/1‑93 (MQ=255)

                                                             cgcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCt                       <  1:755945/93‑1 (MQ=255)

                                                              gcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                      <  2:1571201/93‑1 (MQ=255)

                                                               cggataatCTGAGAAGCGGCCGGCGCGGCGACCAGCGCTCGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTCCCAATTGGCCCCTGa                     <  2:3398555/90‑1 (MQ=255)

                                                               cgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGa                     <  1:3759275/93‑1 (MQ=255)

                                                                gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  1:4855340/1‑93 (MQ=255)

                                                                   taatCTGCGAACCGTCCGGCGCGCCGCCCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACTTc                 <  2:4434911/93‑1 (MQ=255)

                                                                   taatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATc                 >  1:3003110/1‑93 (MQ=255)

                                                                   taatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATc                 <  2:1791182/93‑1 (MQ=255)

                                                                    aatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                <  1:5043266/93‑1 (MQ=255)

                                                                       cTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGcc             <  2:5393788/93‑1 (MQ=255)

                                                                        tGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCAGACATCCGCCt            >  1:5487332/1‑93 (MQ=255)

                                                                           gAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCg         >  2:3979727/1‑93 (MQ=255)

                                                                           gAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCg         >  1:2540391/1‑93 (MQ=255)

                                                                            aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        <  2:6088278/93‑1 (MQ=255)

                                                                            aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        >  2:2336713/1‑93 (MQ=255)

                                                                            aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        >  1:4456462/1‑93 (MQ=255)

                                                                             aCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgag       >  2:2634509/1‑93 (MQ=255)

                                                                              ccGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgaga      >  1:3059758/1‑93 (MQ=255)

                                                                              ccGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgaga      <  1:976426/93‑1 (MQ=255)

                                                                              ccGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgaga      <  1:5821736/93‑1 (MQ=255)

                                                                              ccGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgaga      >  1:1449407/1‑93 (MQ=255)

                                                                               cGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc     >  2:5593783/1‑93 (MQ=255)

                                                                               cGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc     <  2:5731775/93‑1 (MQ=255)

                                                                               cGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc     <  2:23692/93‑1 (MQ=255)

                                                                                gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    >  1:5164076/1‑93 (MQ=255)

                                                                                gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    >  2:278237/1‑93 (MQ=255)

                                                                                gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    >  2:2176208/1‑93 (MQ=255)

||

AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987499‑3987673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  NC_000913/3987493‑3987675

TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                                                           >  SRR4302532.2776337/1‑94 (MQ=60)

TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                                                           >  SRR4302532.148973/1‑94 (MQ=60)

     TAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCG                                                                                      >  SRR4302532.2333679/1‑94 (MQ=60)

      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                                                     >  SRR4302532.3931305/1‑94 (MQ=60)

      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                                                     >  SRR4302532.1454901/1‑94 (MQ=60)

      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                                                     >  SRR4302532.1454902/1‑94 (MQ=60)

        CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                                                                   >  SRR4302532.2835532/1‑94 (MQ=60)

        CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                                                                   >  SRR4302532.3309958/1‑94 (MQ=60)

        CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                                                                   >  SRR4302532.4687717/1‑94 (MQ=60)

          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 >  SRR4302532.928322/1‑94 (MQ=60)

          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 <  SRR4302532.5631713/94‑1 (MQ=60)

          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 >  SRR4302532.3555449/1‑94 (MQ=60)

          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGAGAATAATCTGCGAACCGTCCGGCGCGCCCACCAG                                                                                 >  SRR4302532.921652/1‑94 (MQ=60)

          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTGCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 <  SRR4302532.2154177/94‑1 (MQ=60)

           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                                <  SRR4302532.6290139/94‑1 (MQ=60)

           CTCTGCCAGCGAAATCCCCATTGGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                                <  SRR4302532.893535/94‑1 (MQ=60)

            TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                               <  SRR4302532.5107172/94‑1 (MQ=60)

            TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                               <  SRR4302532.525138/94‑1 (MQ=60)

             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302532.5349249/1‑94 (MQ=60)

             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              <  SRR4302532.5910419/94‑1 (MQ=60)

                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                           >  SRR4302532.683090/1‑94 (MQ=60)

                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                           <  SRR4302532.1066405/94‑1 (MQ=60)

                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                           >  SRR4302532.5098209/1‑94 (MQ=60)

                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          >  SRR4302532.3057277/1‑94 (MQ=60)

                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          >  SRR4302532.2713055/1‑94 (MQ=60)

                  AGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCA                                                                         >  SRR4302532.5960697/1‑94 (MQ=60)

                  AGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCA                                                                         <  SRR4302532.2939251/94‑1 (MQ=60)

                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                        <  SRR4302532.6096226/94‑1 (MQ=60)

                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCCGCGCACGCAGC                                                                       >  SRR4302532.153077/1‑94 (MQ=60)

                      AAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                                                     <  SRR4302532.1508659/94‑1 (MQ=60)

                      AAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                                                     >  SRR4302532.3484889/1‑94 (MQ=60)

                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302532.2946654/1‑94 (MQ=60)

                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302532.577500/1‑94 (MQ=60)

                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302532.5197119/1‑94 (MQ=60)

                        ATACCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302532.5199082/1‑94 (MQ=60)

                        ATACCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGCCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302532.5198936/1‑94 (MQ=60)

                         TCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAT                                                                  <  SRR4302532.4140491/94‑1 (MQ=60)

                          CCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 <  SRR4302532.1409980/94‑1 (MQ=60)

                          CCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 >  SRR4302532.2168516/1‑94 (MQ=60)

                           CCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                                >  SRR4302532.1984902/1‑94 (MQ=60)

                           CCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                                <  SRR4302532.6156092/94‑1 (MQ=60)

                            CCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCTAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               >  SRR4302532.5279183/1‑94 (MQ=60)

                            CCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               <  SRR4302532.2478910/94‑1 (MQ=60)

                            CCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               >  SRR4302532.159173/1‑94 (MQ=60)

                            CCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               >  SRR4302532.4770877/1‑94 (MQ=60)

                             CATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              <  SRR4302532.69563/94‑1 (MQ=60)

                             CATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCC                                                              >  SRR4302532.4718049/1‑94 (MQ=60)

                                TTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCA                                                           <  SRR4302532.1985587/94‑1 (MQ=60)

                                  GTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                                                         >  SRR4302532.4601529/1‑94 (MQ=60)

                                   TTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCA                                                        <  SRR4302532.4159047/94‑1 (MQ=60)

                                   TTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCA                                                        >  SRR4302532.454812/1‑94 (MQ=60)

                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                      >  SRR4302532.4481843/1‑94 (MQ=60)

                                      GGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                                                     <  SRR4302532.3687753/94‑1 (MQ=60)

                                      GGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                                                     <  SRR4302532.1532074/94‑1 (MQ=60)

                                       GCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGGTTTCGCCATCAATAA                                                    >  SRR4302532.3175775/1‑94 (MQ=60)

                                       GCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302532.3263746/94‑1 (MQ=60)

                                        CATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAG                                                   >  SRR4302532.334546/1‑94 (MQ=60)

                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCCCGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302532.2900290/1‑94 (MQ=60)

                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302532.4206732/1‑94 (MQ=60)

                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302532.4206731/1‑94 (MQ=60)

                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302532.2864762/1‑94 (MQ=60)

                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302532.2355889/1‑94 (MQ=60)

                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302532.1863769/1‑94 (MQ=60)

                                          TCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                 <  SRR4302532.4850237/94‑1 (MQ=60)

                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302532.1043029/94‑1 (MQ=60)

                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302532.2227288/94‑1 (MQ=60)

                                             TGCGGCGGACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCACATTTCCCCATCAATAAGCTCGG                                              <  SRR4302532.4668778/94‑1 (MQ=60)

                                             TGCGGCGCACCGGGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCATCCAGATTTCGCCATCAATAAGCTCGG                                              <  SRR4302532.3072466/94‑1 (MQ=60)

                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302532.3480539/1‑94 (MQ=60)

                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       <  SRR4302532.4568835/94‑1 (MQ=60)

                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302532.828406/1‑94 (MQ=60)

                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302532.2447568/1‑94 (MQ=60)

                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302532.3981225/1‑94 (MQ=60)

                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302532.3874746/1‑94 (MQ=60)

                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                    >  SRR4302532.5461780/1‑94 (MQ=60)

                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                    <  SRR4302532.6003279/94‑1 (MQ=60)

                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                    <  SRR4302532.5174038/94‑1 (MQ=60)

                                                        GCGGAGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCA                                   >  SRR4302532.4368305/1‑94 (MQ=60)

                                                         CGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAA                                  <  SRR4302532.555652/94‑1 (MQ=60)

                                                         CGGCGTTCACCGCGAATAATCTGCGAACAGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAA                                  >  SRR4302532.4080193/1‑94 (MQ=60)

                                                          GGGGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 <  SRR4302532.3771871/94‑1 (MQ=60)

                                                          GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 <  SRR4302532.5942621/94‑1 (MQ=60)

                                                          GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 >  SRR4302532.2299687/1‑94 (MQ=60)

                                                          GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 <  SRR4302532.5942785/94‑1 (MQ=60)

                                                          GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 >  SRR4302532.2327819/1‑94 (MQ=60)

                                                           GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302532.3671880/94‑1 (MQ=60)

                                                           GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302532.2285469/94‑1 (MQ=60)

                                                           GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302532.4159630/94‑1 (MQ=60)

                                                            GGTTCTCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302532.1119645/94‑1 (MQ=60)

                                                            CGTTCACGGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302532.6079766/94‑1 (MQ=60)

                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               >  SRR4302532.3004708/1‑94 (MQ=60)

                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302532.3434425/94‑1 (MQ=60)

                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302532.4936464/94‑1 (MQ=60)

                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302532.933768/94‑1 (MQ=60)

                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302532.4152478/1‑94 (MQ=60)

                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302532.2779227/1‑94 (MQ=60)

                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              <  SRR4302532.4542259/94‑1 (MQ=60)

                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAACAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTTG                              >  SRR4302532.1077562/1‑94 (MQ=60)

                                                              TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                             >  SRR4302532.969862/1‑94 (MQ=60)

                                                              TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                             <  SRR4302532.1655371/94‑1 (MQ=60)

                                                               TCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCA                            >  SRR4302532.5104865/1‑94 (MQ=60)

                                                               TCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCA                            <  SRR4302532.3337503/94‑1 (MQ=60)

                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           >  SRR4302532.269208/1‑94 (MQ=60)

                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           >  SRR4302532.269206/1‑94 (MQ=60)

                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           >  SRR4302532.269207/1‑94 (MQ=60)

                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           <  SRR4302532.3295334/94‑1 (MQ=60)

                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           >  SRR4302532.1247148/1‑94 (MQ=60)

                                                                  CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCTATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                         <  SRR4302532.729146/94‑1 (MQ=60)

                                                                  CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                         >  SRR4302532.5452504/1‑94 (MQ=60)

                                                                  CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                         <  SRR4302532.4119472/94‑1 (MQ=60)

                                                                   CGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTG                        <  SRR4302532.781839/94‑1 (MQ=60)

                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      >  SRR4302532.5022404/1‑94 (MQ=60)

                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302532.3885753/94‑1 (MQ=60)

                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCGGAC                      <  SRR4302532.97500/94‑1 (MQ=60)

                                                                        ATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATC                   >  SRR4302532.3102298/1‑94 (MQ=60)

                                                                         TAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCC                  >  SRR4302532.2584622/1‑94 (MQ=60)

                                                                         TAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCC                  <  SRR4302532.5184925/94‑1 (MQ=60)

                                                                          AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 >  SRR4302532.311168/1‑94 (MQ=60)

                                                                          AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 <  SRR4302532.5216275/94‑1 (MQ=60)

                                                                           ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302532.5554361/1‑94 (MQ=60)

                                                                           ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302532.655656/1‑94 (MQ=60)

                                                                           ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302532.1984662/1‑94 (MQ=60)

                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT              >  SRR4302532.3587514/1‑94 (MQ=60)

                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCAGACATCCGCCT              >  SRR4302532.5677600/1‑94 (MQ=60)

                                                                              cgccAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTT             <  SRR4302532.1808116/90‑1 (MQ=60)

                                                                               GCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTC            <  SRR4302532.6058831/94‑1 (MQ=60)

                                                                                CGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCG           >  SRR4302532.2625125/1‑94 (MQ=60)

                                                                                CGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCG           >  SRR4302532.4943956/1‑94 (MQ=60)

                                                                                 GAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA          >  SRR4302532.3090505/1‑94 (MQ=60)

                                                                                 GAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA          >  SRR4302532.4608359/1‑94 (MQ=60)

                                                                                   ACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGA        >  SRR4302532.1498275/1‑94 (MQ=60)

                                                                                   ACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGA        >  SRR4302532.3160789/1‑94 (MQ=60)

                                                                                    CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC       <  SRR4302532.6023917/94‑1 (MQ=60)

                                                                                    CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC       <  SRR4302532.1009759/94‑1 (MQ=60)

                                                                                     CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      >  SRR4302532.5341840/1‑94 (MQ=60)

                                                                                      GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTAGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG     >  SRR4302532.5447762/1‑94 (MQ=60)

                                                                                        CCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT   >  SRR4302532.5738719/1‑94 (MQ=60)

                                                                                        CCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT   <  SRR4302532.3714528/94‑1 (MQ=60)

                                                                                         CGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTTACATCCGCCTTCGAGACGGGTA  >  SRR4302532.3293219/1‑94 (MQ=60)

TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  NC_000913/3987493‑3987675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`