Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,2891.G100.0% 699.0 / NA 193L127P (CTG→CCG) b4566hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (109/84);  total (109/84)

Only 100 of 216 total aligned reads displayed.

GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376
                                                                                             |                                                                                        
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                           <  1:5635226/93‑1 (MQ=255)
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                           <  1:1328206/93‑1 (MQ=255)
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                           <  1:1712167/93‑1 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                         >  2:5499598/1‑93 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                         >  2:2644010/1‑93 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                         <  2:5258777/93‑1 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCAA‑g                                                                                         >  2:1973097/1‑93 (MQ=255)
  cTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gg                                                                                        >  1:3971482/1‑92 (MQ=255)
   cgCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gga                                                                                       <  2:4990243/92‑3 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                      >  1:696906/1‑90 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                      <  2:1972155/93‑4 (MQ=255)
     cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggacg                                                                                     >  2:4511728/1‑89 (MQ=255)
     cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggacg                                                                                     <  2:4440440/93‑5 (MQ=255)
     cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggacg                                                                                     <  2:5796335/93‑5 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGa                                                                                   >  1:1571054/1‑93 (MQ=255)
        cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGAt                                                                                  >  1:591351/1‑93 (MQ=255)
         gCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATa                                                                                 <  2:3562809/93‑1 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAg                                                                              >  2:4568982/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAg                                                                              >  1:1789415/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAg                                                                              >  2:5528021/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAg                                                                              <  1:860584/93‑1 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAg                                                                              >  2:5825273/1‑93 (MQ=255)
             aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGa                                                                             <  2:3285954/93‑1 (MQ=255)
             aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCCACATAAAACACGCCGCCAGG‑ACGGATAAAGa                                                                             >  2:5072575/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGaa                                                                            >  2:4155906/1‑93 (MQ=255)
               tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGaaa                                                                           <  1:1154236/93‑1 (MQ=255)
                gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAt                                                                          >  2:1245867/1‑93 (MQ=255)
                  aTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTc                                                                        >  2:654569/1‑93 (MQ=255)
                   tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTcc                                                                       <  1:293892/93‑1 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTccc                                                                      <  1:5090373/93‑1 (MQ=255)
                     tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCt                                                                     >  2:2952643/1‑93 (MQ=255)
                      ctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCtt                                                                    <  1:5832206/93‑1 (MQ=255)
                       tttAGTGGTTTTGAAGGCATCCAGACGAACCATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCGA‑GCACGGATAACGAAGTTCCCttt                                                                   >  1:3739607/1‑92 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTc                                                                   >  2:1360929/1‑93 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTc                                                                   >  2:3078234/1‑93 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTc                                                                   <  1:3180790/93‑1 (MQ=255)
                        ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCa                                                                  <  1:5765551/93‑1 (MQ=255)
                          aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCAcc                                                                >  2:3023099/1‑93 (MQ=255)
                          aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCAcc                                                                <  1:3249322/93‑1 (MQ=255)
                           cTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCt                                                               <  2:3739674/93‑1 (MQ=255)
                           cTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCt                                                               >  1:430062/1‑93 (MQ=255)
                            tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTg                                                              >  2:3986301/1‑93 (MQ=255)
                            tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTg                                                              >  2:5594334/1‑93 (MQ=255)
                              gTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGaa                                                            <  2:2416226/93‑1 (MQ=255)
                               ttttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAg                                                           >  1:1835307/1‑93 (MQ=255)
                                   gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                       >  2:3735396/1‑93 (MQ=255)
                                      ggCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAtt                                                    >  1:1004224/1‑93 (MQ=255)
                                       gCATCCAGCCGAGCGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCCCCTGAAGCGAGAttt                                                   <  1:3297478/93‑1 (MQ=255)
                                        cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTa                                                  <  1:942630/93‑1 (MQ=255)
                                          tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAc                                                >  1:2753039/1‑93 (MQ=255)
                                           ccAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAcc                                               >  2:5020598/1‑92 (MQ=255)
                                            cAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGc                                              <  2:184354/93‑1 (MQ=255)
                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                             >  2:1667708/1‑93 (MQ=255)
                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                             >  2:3675634/1‑93 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  1:4671524/1‑93 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  1:6131783/1‑93 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  1:1256320/1‑93 (MQ=255)
                                               acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAc                                           <  1:5793530/93‑1 (MQ=255)
                                                 gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                         >  2:5698776/1‑93 (MQ=255)
                                                  aacgaTGCCGCCTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        <  2:4332744/93‑1 (MQ=255)
                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        >  2:2463816/1‑93 (MQ=255)
                                                    cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                      >  1:1594897/1‑93 (MQ=255)
                                                    cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                      <  1:2872189/93‑1 (MQ=255)
                                                    cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                      <  2:1552288/93‑1 (MQ=255)
                                                    cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                      <  2:2019583/93‑1 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    >  1:2842281/1‑93 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    >  2:2471379/1‑93 (MQ=255)
                                                          cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                >  2:1388244/1‑93 (MQ=255)
                                                            aCTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                              >  1:4749325/1‑93 (MQ=255)
                                                              tttATCGCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtt                            <  2:2134740/93‑1 (MQ=255)
                                                               ttATCTCCCGCGACATACAGCACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           <  2:5453192/93‑1 (MQ=255)
                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           >  1:3423079/1‑93 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          <  2:5370405/93‑1 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          <  2:256956/93‑1 (MQ=255)
                                                                 aTCTCCCGCAACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         <  1:2362459/93‑1 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTca                        >  2:4673864/1‑92 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTCTCg                        >  2:3174009/1‑93 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       <  2:4857955/93‑1 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGG‑ACGGAGAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       <  2:5931943/93‑1 (MQ=255)
                                                                    tcCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAt                      <  1:3252680/93‑1 (MQ=255)
                                                                      ccGCGCCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGt                    <  1:1357433/93‑1 (MQ=255)
                                                                      ccGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGt                    <  2:4737377/93‑1 (MQ=255)
                                                                        gcgACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                  <  2:4682551/93‑1 (MQ=255)
                                                                         cgACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACa                 <  1:5505831/93‑1 (MQ=255)
                                                                          gACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                >  1:4349542/1‑93 (MQ=255)
                                                                           aCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa               <  2:2898757/93‑1 (MQ=255)
                                                                           aCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa               >  1:4160831/1‑93 (MQ=255)
                                                                           aCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa               <  1:3726579/93‑1 (MQ=255)
                                                                            cATAAAACAAGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc              >  2:4972595/1‑93 (MQ=255)
                                                                              tAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg            >  1:2538520/1‑93 (MQ=255)
                                                                              tAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCCGACCGTTTTCGATGTACAAACCg            >  1:3502798/1‑93 (MQ=255)
                                                                               aaaaCACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGa           <  1:40147/93‑1 (MQ=255)
                                                                                 aaCACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc         >  2:47850/1‑93 (MQ=255)
                                                                                 aaCACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAACTTCTGCTGACCGTGTTCGAAGTACAAACCGAgc         >  2:6101965/1‑93 (MQ=255)
                                                                                  acacGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGAACAAACCGAgcg        >  2:2505681/1‑93 (MQ=255)
                                                                                  acacGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGATTTCGATGTACAAACCGAgcg        <  1:6038418/93‑1 (MQ=255)
                                                                                    acGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc      <  1:1645394/93‑1 (MQ=255)
                                                                                       ccgccAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa   >  2:1147602/1‑93 (MQ=255)
                                                                                        cgccAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTTCAAACCGAGCGGCGCAt  <  1:3638168/93‑1 (MQ=255)
                                                                                        cgccAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAt  >  1:242206/1‑93 (MQ=255)
                                                                                             |                                                                                        
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTC  >  NC_000913/3998197‑3998383
                                                                                              |                                                                                              
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                                <  SRR4302532.1769883/94‑1 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                                <  SRR4302532.5830965/94‑1 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                                <  SRR4302532.1372594/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑G                                                                                              >  SRR4302532.4105265/1‑94 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                             >  SRR4302532.3258054/1‑91 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                             >  SRR4302532.720764/1‑91 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                             >  SRR4302532.720763/1‑91 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGA                                                                                          <  SRR4302532.4232085/94‑1 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGA                                                                                          <  SRR4302532.4660044/94‑1 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGA                                                                                          >  SRR4302532.1624228/1‑94 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGA                                                                                          >  SRR4302532.1624227/1‑94 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGA                                                                                          >  SRR4302532.2958349/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGAT                                                                                         >  SRR4302532.611986/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGAT                                                                                         >  SRR4302532.3367926/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAG                                                                                     >  SRR4302532.2928306/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAG                                                                                     >  SRR4302532.1849213/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAG                                                                                     >  SRR4302532.3531333/1‑94 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGA                                                                                    >  SRR4302532.3017129/1‑94 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGA                                                                                    <  SRR4302532.890138/94‑1 (MQ=60)
             ACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGCCGAACGATGCCGACTTTACCTCCCTCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAA                                                                                   <  SRR4302532.537789/94‑1 (MQ=60)
             ACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAA                                                                                   <  SRR4302532.537788/94‑1 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAT                                                                                 <  SRR4302532.1811758/94‑1 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAT                                                                                 <  SRR4302532.1192744/94‑1 (MQ=60)
                   TCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCC                                                                             <  SRR4302532.304022/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCT                                                                            <  SRR4302532.5265412/94‑1 (MQ=60)
                     TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTT                                                                           >  SRR4302532.4182868/1‑94 (MQ=60)
                      CTTTAGTGGTTTTGAAGGCATCCAGACGAACCATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCGA‑GCACGGATAACGAAGTTCCCTTT                                                                          >  SRR4302532.3865272/1‑94 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTC                                                                          <  SRR4302532.6034679/94‑1 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTC                                                                          <  SRR4302532.4811493/94‑1 (MQ=60)
                       TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCA                                                                         <  SRR4302532.3286424/94‑1 (MQ=60)
                        TTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCAC                                                                        <  SRR4302532.5966179/94‑1 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACC                                                                       >  SRR4302532.3743855/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCT                                                                      <  SRR4302532.5153015/94‑1 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCT                                                                      >  SRR4302532.444965/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCT                                                                      <  SRR4302532.3357209/94‑1 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGA                                                                    >  SRR4302532.1274441/1‑94 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAG                                                                  >  SRR4302532.1896322/1‑94 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAG                                                                  <  SRR4302532.602450/94‑1 (MQ=60)
                                     AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                           >  SRR4302532.3766269/1‑94 (MQ=60)
                                     AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                           >  SRR4302532.1038401/1‑94 (MQ=60)
                                     AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                           >  SRR4302532.1764592/1‑94 (MQ=60)
                                       GCATCCAGCCGAGCGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCCCCTGAAGCGAGATTTA                                                         <  SRR4302532.3407197/94‑1 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                        <  SRR4302532.974916/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                       >  SRR4302532.2845503/1‑94 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                       <  SRR4302532.3352915/94‑1 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCC                                                    >  SRR4302532.3822367/1‑94 (MQ=60)
                                             AGACGAACGATGCTGACTTTATCTCCCGCGACATAAAACACCCCGACACG‑CCGGATAAAGAACTTCCCTTCACCTGAAGCGAGATTTAACGtaa                                                   >  SRR4302532.3446447/1‑91 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                   >  SRR4302532.4830797/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                   >  SRR4302532.489897/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                   >  SRR4302532.6343344/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                   <  SRR4302532.142881/94‑1 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                   >  SRR4302532.1298340/1‑94 (MQ=60)
                                               ACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACC                                                 <  SRR4302532.5994934/94‑1 (MQ=60)
                                               ACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACC                                                 <  SRR4302532.3539470/94‑1 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACTTGAAGCGAGATTTAACGCCACCT                                                >  SRR4302532.4448134/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                              >  SRR4302532.597997/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                              >  SRR4302532.1660631/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                             >  SRR4302532.1648884/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                            <  SRR4302532.111149/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                            >  SRR4302532.5970023/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                            <  SRR4302532.2968224/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                            >  SRR4302532.4088082/1‑94 (MQ=60)
                                                    AGATGCCGACTTTCGCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                            <  SRR4302532.868370/94‑1 (MQ=60)
                                                     GATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGC                                           >  SRR4302532.2937283/1‑94 (MQ=60)
                                                     GATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGC                                           >  SRR4302532.605944/1‑94 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                          <  SRR4302532.897890/94‑1 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                      >  SRR4302532.2368014/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                     >  SRR4302532.4911660/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                   >  SRR4302532.1678311/1‑94 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                  >  SRR4302532.3537745/1‑94 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                 >  SRR4302532.2749822/1‑94 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                                >  SRR4302532.82309/1‑94 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                                <  SRR4302532.1383028/94‑1 (MQ=60)
                                                                 ATCTCCCGCAACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                               <  SRR4302532.2441261/94‑1 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACAGTTTTCGAT                             >  SRR4302532.1876434/1‑94 (MQ=60)
                                                                    TCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                            <  SRR4302532.3360732/94‑1 (MQ=60)
                                                                      CCGCGCCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTA                          <  SRR4302532.1402878/94‑1 (MQ=60)
                                                                        GCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                        <  SRR4302532.3357546/94‑1 (MQ=60)
                                                                        GCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                        <  SRR4302532.4613597/94‑1 (MQ=60)
                                                                        GCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                        <  SRR4302532.2596041/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                       >  SRR4302532.5704870/1‑94 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                       <  SRR4302532.5696812/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                       >  SRR4302532.4496575/1‑94 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                       <  SRR4302532.6264231/94‑1 (MQ=60)
                                                                          GACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                      >  SRR4302532.4300386/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                     <  SRR4302532.2496605/94‑1 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                     <  SRR4302532.3851748/94‑1 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                     <  SRR4302532.3851749/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                   >  SRR4302532.2623193/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                   >  SRR4302532.4434441/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCCGACCGTTTTCGATGTACAAACCG                   >  SRR4302532.3620130/1‑94 (MQ=60)
                                                                              TAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                  <  SRR4302532.1337885/94‑1 (MQ=60)
                                                                              TAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                  >  SRR4302532.4109032/1‑94 (MQ=60)
                                                                               AAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                 <  SRR4302532.41593/94‑1 (MQ=60)
                                                                               AAAACACGACGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                 >  SRR4302532.6202709/1‑94 (MQ=60)
                                                                                  ACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGATTTCGATGTACAAACCGAGCGG              <  SRR4302532.6246968/94‑1 (MQ=60)
                                                                                    ACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG            <  SRR4302532.1701133/94‑1 (MQ=60)
                                                                                       CCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT         >  SRR4302532.3309652/1‑94 (MQ=60)
                                                                                       CCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT         >  SRR4302532.2547868/1‑94 (MQ=60)
                                                                                       CCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT         >  SRR4302532.250575/1‑94 (MQ=60)
                                                                                        CGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTTCAAACCGAGCGGCGCATA        <  SRR4302532.3760234/94‑1 (MQ=60)
                                                                                        CGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA        >  SRR4302532.6351261/1‑94 (MQ=60)
                                                                                        CGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA        <  SRR4302532.585441/94‑1 (MQ=60)
                                                                                         GCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG       >  SRR4302532.1844530/1‑94 (MQ=60)
                                                                                          CCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC      >  SRR4302532.2551236/1‑94 (MQ=60)
                                                                                            G‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT   >  SRR4302532.248452/1‑94 (MQ=60)
                                                                                            G‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT   >  SRR4302532.3988226/1‑94 (MQ=60)
                                                                                            G‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT   <  SRR4302532.336667/94‑1 (MQ=60)
                                                                                              G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAACGCGCGGCGCATAGCTTTC  >  SRR4302532.1625474/1‑94 (MQ=60)
                                                                                              |                                                                                              
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTC  >  NC_000913/3998197‑3998383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑