Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,984 +CG coding (1516/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9831.G99.4% 612.6 / ‑4.1 174coding (1515/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (90/83);  total (90/84)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
*NC_0009134,004,9832.C99.4% 611.7 / ‑3.9 174coding (1515/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (90/83);  total (90/84)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 192 total aligned reads displayed.

CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004893‑4005060
                                                                                               ||                                                                             
 cGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gc                                                                             >  1:2253464/1‑92 (MQ=255)
 cGCCAGCTGATTCACCTCGGCCTGGTAACGCAAAATATTGCCCAGAATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gc                                                                             <  1:4748485/93‑2 (MQ=255)
  gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcg                                                                            >  2:2213893/1‑91 (MQ=255)
  gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcg                                                                            >  1:5045380/1‑91 (MQ=255)
   ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAAGTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcga                                                                           >  1:5149951/1‑90 (MQ=255)
   ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcga                                                                           >  1:1698713/1‑90 (MQ=255)
   ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCACTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcga                                                                           >  2:1108254/1‑90 (MQ=255)
    cAGCTGATTCACCTCGGCCTGGTGATGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcgaa                                                                          <  2:3151120/93‑5 (MQ=255)
          aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCctct                                                                      >  2:5530869/1‑93 (MQ=255)
          aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCctct                                                                      <  1:609470/93‑1 (MQ=255)
           ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCtt                                                                     <  2:2150175/93‑1 (MQ=255)
           ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCtt                                                                     >  1:5648801/1‑93 (MQ=255)
           ttCACCTCGGCCGGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCtt                                                                     <  1:3918640/93‑1 (MQ=255)
            tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCttt                                                                    <  2:4258183/93‑1 (MQ=255)
              cccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGc                                                                  <  2:1764047/92‑1 (MQ=255)
              aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGc                                                                  >  2:3015734/1‑93 (MQ=255)
                 tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  2:5626980/1‑93 (MQ=255)
                 tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  2:647907/1‑93 (MQ=255)
                  cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACt                                                              <  1:485284/93‑1 (MQ=255)
                   ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACtt                                                             <  1:2437194/93‑1 (MQ=255)
                    gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                            >  2:4415834/1‑93 (MQ=255)
                      cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  1:2557025/1‑93 (MQ=255)
                      cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  2:1945712/1‑93 (MQ=255)
                       tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         <  1:1366513/93‑1 (MQ=255)
                       tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         >  1:4225600/1‑93 (MQ=255)
                        ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                        <  2:3845482/93‑1 (MQ=255)
                        ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                        <  2:5968971/93‑1 (MQ=255)
                           gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     <  2:3048351/93‑1 (MQ=255)
                           gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     >  1:737909/1‑93 (MQ=255)
                              gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCcgcg                                                  >  2:4896244/1‑93 (MQ=255)
                               cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt                                                 >  1:2814729/1‑93 (MQ=255)
                                aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:4664803/1‑93 (MQ=255)
                                aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  1:478854/1‑93 (MQ=255)
                                aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:1683698/1‑93 (MQ=255)
                                aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                <  2:3710425/93‑1 (MQ=255)
                                 aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  2:3489286/93‑1 (MQ=255)
                                  aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  2:4431861/93‑1 (MQ=255)
                                  aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  2:3127768/1‑93 (MQ=255)
                                   atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             <  2:2935356/93‑1 (MQ=255)
                                   atatTCCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             <  1:2727634/93‑1 (MQ=255)
                                    tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            >  1:5610065/1‑93 (MQ=255)
                                    tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            >  2:2648882/1‑93 (MQ=255)
                                     atTGCCCAGCATTGTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  2:498025/93‑1 (MQ=255)
                                     atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTCTCGTg                                           >  2:6066578/1‑93 (MQ=255)
                                     atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           >  1:4110116/1‑93 (MQ=255)
                                     atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGAATCGTg                                           >  1:1030131/1‑93 (MQ=255)
                                       tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:68579/93‑1 (MQ=255)
                                         cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       >  1:4983243/1‑93 (MQ=255)
                                         cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       >  1:1902402/1‑93 (MQ=255)
                                          ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:4326206/1‑93 (MQ=255)
                                          ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:5797120/1‑93 (MQ=255)
                                            aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:1363504/1‑93 (MQ=255)
                                            aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:42412/1‑93 (MQ=255)
                                              cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  <  1:5977428/93‑1 (MQ=255)
                                              cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  <  1:3342355/93‑1 (MQ=255)
                                               aTTCTGCCATACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  1:1477040/93‑1 (MQ=255)
                                                ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  2:2582152/93‑1 (MQ=255)
                                                 tCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCg                               <  1:4527807/93‑1 (MQ=255)
                                                  cTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              >  2:4790687/1‑93 (MQ=255)
                                                   tGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  2:3797509/1‑93 (MQ=255)
                                                   tGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             <  2:4809197/93‑1 (MQ=255)
                                                     cccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:3400904/93‑1 (MQ=255)
                                                     cccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:141115/93‑1 (MQ=255)
                                                     cccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  1:5771225/93‑1 (MQ=255)
                                                     cccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:141302/93‑1 (MQ=255)
                                                      ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  1:4644784/93‑1 (MQ=255)
                                                      ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:892257/1‑93 (MQ=255)
                                                      ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  2:368662/1‑93 (MQ=255)
                                                      ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  2:5234938/1‑93 (MQ=255)
                                                       cTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGTCGCTCAAACCGAAAGCg                         >  2:201437/1‑93 (MQ=255)
                                                       cTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         >  1:3724428/1‑93 (MQ=255)
                                                       cTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  1:6040366/93‑1 (MQ=255)
                                                        tACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:1510820/1‑93 (MQ=255)
                                                          cAACTGGCAGAGGCCGCGCGCCCGATGCTGCT‑CGGC‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATg                      <  2:2111542/93‑1 (MQ=255)
                                                          cAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATg                      >  1:2987551/1‑93 (MQ=255)
                                                           aaCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     >  2:5389368/1‑93 (MQ=255)
                                                           aaCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  2:1693106/93‑1 (MQ=255)
                                                            aCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  2:3896197/93‑1 (MQ=255)
                                                            aCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  1:1233682/93‑1 (MQ=255)
                                                              tGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                  >  2:3787711/1‑93 (MQ=255)
                                                               gACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAATGCGATGCAGaa                 <  1:3685651/93‑1 (MQ=255)
                                                               gACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 <  2:2396815/93‑1 (MQ=255)
                                                                aCAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  1:3131271/93‑1 (MQ=255)
                                                                 cAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  1:3622664/93‑1 (MQ=255)
                                                                   gagGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  1:3996234/1‑93 (MQ=255)
                                                                     ggCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:2665705/93‑1 (MQ=255)
                                                                     ggCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  1:5082222/93‑1 (MQ=255)
                                                                     ggCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  2:3173523/1‑93 (MQ=255)
                                                                      gCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:6071625/93‑1 (MQ=255)
                                                                      gCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:5869253/93‑1 (MQ=255)
                                                                        cgcgcgCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg        >  2:4038532/1‑93 (MQ=255)
                                                                        cgcgcgCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg        <  1:1631922/93‑1 (MQ=255)
                                                                         gcgcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:1830231/93‑1 (MQ=255)
                                                                          cgcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:6141102/93‑1 (MQ=255)
                                                                           gcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg     >  1:3410942/1‑93 (MQ=255)
                                                                            cgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgga    >  1:2500519/1‑92 (MQ=255)
                                                                              cccG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  1:1046045/1‑93 (MQ=255)
                                                                              cccG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  <  1:5406918/93‑1 (MQ=255)
                                                                              cccG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  2:1215068/1‑93 (MQ=255)
                                                                              cccG‑‑G‑TGCTGCGCGGCGAATCCTATTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  2:5425263/1‑93 (MQ=255)
                                                                                               ||                                                                             
CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004893‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG  >  NC_000913/4004900‑4005076
                                                                                          |                                                                                            
CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCT                                                                                        >  SRR4302532.4826974/1‑94 (MQ=60)
 TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTC                                                                                       >  SRR4302532.271349/1‑94 (MQ=60)
   ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTT                                                                                     <  SRR4302532.630719/94‑1 (MQ=60)
   ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTT                                                                                     >  SRR4302532.5845324/1‑94 (MQ=60)
    TTCACCTCGGCCGGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTT                                                                                    <  SRR4302532.4050680/94‑1 (MQ=60)
     TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTG                                                                                   <  SRR4302532.3179821/94‑1 (MQ=60)
      CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGC                                                                                  <  SRR4302532.4082946/94‑1 (MQ=60)
       CCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCA                                                                                 <  SRR4302532.1944241/94‑1 (MQ=60)
          TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACT                                                                              <  SRR4302532.2931101/94‑1 (MQ=60)
           CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTT                                                                             <  SRR4302532.502225/94‑1 (MQ=60)
            GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTG                                                                            <  SRR4302532.2518226/94‑1 (MQ=60)
             GCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGC                                                                           <  SRR4302532.5056790/94‑1 (MQ=60)
              CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCC                                                                          >  SRR4302532.2642310/1‑94 (MQ=60)
               CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCG                                                                         <  SRR4302532.3846914/94‑1 (MQ=60)
               CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCG                                                                         >  SRR4302532.4367436/1‑94 (MQ=60)
               CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCG                                                                         <  SRR4302532.2396662/94‑1 (MQ=60)
                TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGT                                                                        <  SRR4302532.1412277/94‑1 (MQ=60)
                 GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                                       >  SRR4302532.5860210/1‑94 (MQ=60)
                  GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGC                                                                      >  SRR4302532.1612587/1‑94 (MQ=60)
                   TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                                     >  SRR4302532.763042/1‑94 (MQ=60)
                       GCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGT                                                                 >  SRR4302532.2908941/1‑94 (MQ=60)
                        CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                                >  SRR4302532.495549/1‑94 (MQ=60)
                        CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                                >  SRR4302532.1293676/1‑94 (MQ=60)
                         AAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAT                                                               <  SRR4302532.3752521/94‑1 (MQ=60)
                           AATATTGCTGAGCATTCTGCCCTACAACTGACAGAGGCAGCGCGCCAGGTGC‑TGGCCGCC‑‑GAATCCTCTTTGCATCTTGCCGTGCCGCGGATCG                                                             <  SRR4302532.2848008/94‑1 (MQ=48)
                            ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                                            >  SRR4302532.5804768/1‑94 (MQ=60)
                            ATATTCCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                                            <  SRR4302532.2819346/94‑1 (MQ=60)
                             TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                                           >  SRR4302532.4248085/1‑94 (MQ=60)
                             TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGAATCGTG                                                           >  SRR4302532.1064936/1‑94 (MQ=60)
                              ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                                          <  SRR4302532.4916073/94‑1 (MQ=60)
                               TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                                         <  SRR4302532.5222519/94‑1 (MQ=60)
                                 GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                                       >  SRR4302532.1965483/1‑94 (MQ=60)
                                 GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                                       >  SRR4302532.5154066/1‑94 (MQ=60)
                                  CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                      >  SRR4302532.4472379/1‑94 (MQ=60)
                                   CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                                     <  SRR4302532.3398737/94‑1 (MQ=60)
                                    CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                                    >  SRR4302532.4814527/1‑94 (MQ=60)
                                     AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                                   >  SRR4302532.5169634/1‑94 (MQ=60)
                                      GCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAA                                                  >  SRR4302532.917104/1‑94 (MQ=60)
                                      CCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGAGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCccgga                                                  >  SRR4302532.307188/1‑89 (MQ=60)
                                       CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                 <  SRR4302532.6184021/94‑1 (MQ=60)
                                       CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                 <  SRR4302532.6184022/94‑1 (MQ=60)
                                       CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                 >  SRR4302532.348731/1‑94 (MQ=60)
                                       CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                 <  SRR4302532.3453906/94‑1 (MQ=60)
                                       CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                 >  SRR4302532.5639170/1‑94 (MQ=60)
                                       CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                 >  SRR4302532.2879525/1‑94 (MQ=60)
                                        ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                >  SRR4302532.6313159/1‑94 (MQ=60)
                                        ATTCTGCCATACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                <  SRR4302532.1526821/94‑1 (MQ=60)
                                         TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                               >  SRR4302532.5990555/1‑94 (MQ=60)
                                          TCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGA                                              <  SRR4302532.4682352/94‑1 (MQ=60)
                                              CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                          >  SRR4302532.922726/1‑94 (MQ=60)
                                              CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                          >  SRR4302532.2521993/1‑94 (MQ=60)
                                              CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                          <  SRR4302532.5972042/94‑1 (MQ=60)
                                               CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                         >  SRR4302532.2629425/1‑94 (MQ=60)
                                               CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                         <  SRR4302532.4803146/94‑1 (MQ=60)
                                               CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                         >  SRR4302532.3849496/1‑94 (MQ=60)
                                                CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                        >  SRR4302532.2051212/1‑94 (MQ=60)
                                                CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                        <  SRR4302532.6248962/94‑1 (MQ=60)
                                                CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                        >  SRR4302532.1561836/1‑94 (MQ=60)
                                                 TACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                       >  SRR4302532.1748103/1‑94 (MQ=60)
                                                 TACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                       <  SRR4302532.4309249/94‑1 (MQ=60)
                                                  ACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                                      >  SRR4302532.3086317/1‑94 (MQ=60)
                                                    AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                                    <  SRR4302532.5542928/94‑1 (MQ=60)
                                                    AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                                    >  SRR4302532.5307841/1‑94 (MQ=60)
                                                     ACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                                   <  SRR4302532.1274958/94‑1 (MQ=60)
                                                     ACTGACAGAGGCCGCCCCCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCGGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                                   <  SRR4302532.1551755/94‑1 (MQ=60)
                                                        GACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAATGCGATGCAGAAA                                <  SRR4302532.3809417/94‑1 (MQ=60)
                                                         ACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                               <  SRR4302532.3235216/94‑1 (MQ=60)
                                                          CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                              <  SRR4302532.3744020/94‑1 (MQ=60)
                                                          CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                              <  SRR4302532.354534/94‑1 (MQ=60)
                                                           AGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                             >  SRR4302532.4130855/1‑94 (MQ=60)
                                                            cctGCCGCGCGCCCGGTGCGG‑‑‑CGCGGCGAATCCTCTTTGCAACTTCCCGTGCCGCGTATCGTGGCCCTCAATCCGAAAGCCATGCAGCCATCGT                            <  SRR4302532.434318/91‑1 (MQ=37)
                                                            GAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT                            <  SRR4302532.6277042/94‑1 (MQ=60)
                                                             AGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT                           <  SRR4302532.4180957/94‑1 (MQ=60)
                                                              GGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC                          <  SRR4302532.5256968/94‑1 (MQ=60)
                                                              GGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC                          <  SRR4302532.4027711/94‑1 (MQ=60)
                                                               GCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG                         <  SRR4302532.6281310/94‑1 (MQ=60)
                                                                 CGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                       >  SRR4302532.717551/1‑94 (MQ=60)
                                                                 CGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                       <  SRR4302532.1687164/94‑1 (MQ=60)
                                                                  GCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG                      >  SRR4302532.4611505/1‑94 (MQ=60)
                                                                   CGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG                     >  SRR4302532.3525170/1‑94 (MQ=60)
                                                                   CGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG                     <  SRR4302532.6352941/94‑1 (MQ=60)
                                                                    GCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC                    <  SRR4302532.4779535/94‑1 (MQ=60)
                                                                    GCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGA                    >  SRR4302532.2583901/1‑94 (MQ=60)
                                                                      GCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA                  >  SRR4302532.1081302/1‑94 (MQ=60)
                                                                       CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC                 >  SRR4302532.5024543/1‑94 (MQ=60)
                                                                       CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC                 >  SRR4302532.2738554/1‑94 (MQ=60)
                                                                       CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC                 <  SRR4302532.5594246/94‑1 (MQ=60)
                                                                       CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCAGCAAC                 >  SRR4302532.2738553/1‑94 (MQ=60)
                                                                        CCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                >  SRR4302532.992749/1‑94 (MQ=60)
                                                                        CCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                <  SRR4302532.2422984/94‑1 (MQ=60)
                                                                        CCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATATATCGGCGGCAACT                >  SRR4302532.4610544/1‑94 (MQ=60)
                                                                         CGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA               >  SRR4302532.362914/1‑94 (MQ=60)
                                                                           ctggtgctgcgcggcGAATCCTCTTTGCACCTTGCCGTGCCGCGTATCGTGGCGCTCAACCCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                <  SRR4302532.4007323/79‑1 (MQ=60)
                                                                           cgggtcctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCACCTATCGTGGGGCTCAAACCGTAACCGTTGCACAAATAGTTCGGCGCCAACT                >  SRR4302532.2411550/16‑94 (MQ=26)
                                                                             ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT              <  SRR4302532.1784942/81‑1 (MQ=60)
                                                                             ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT              <  SRR4302532.2136159/81‑1 (MQ=60)
                                                                             ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT              >  SRR4302532.3096320/14‑94 (MQ=60)
                                                                             ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT              >  SRR4302532.5648474/14‑94 (MQ=60)
                                                                              gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG             <  SRR4302532.4829740/82‑1 (MQ=60)
                                                                              gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG             <  SRR4302532.2499782/82‑1 (MQ=60)
                                                                              gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG             <  SRR4302532.4978125/82‑1 (MQ=60)
                                                                              gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG             <  SRR4302532.4635490/82‑1 (MQ=60)
                                                                              gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG             <  SRR4302532.5234405/82‑1 (MQ=60)
                                                                               tgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGA            >  SRR4302532.3483421/12‑94 (MQ=60)
                                                                                gctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGAT           <  SRR4302532.5629878/84‑1 (MQ=60)
                                                                                gctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGAT           <  SRR4302532.4474299/84‑1 (MQ=60)
                                                                                 gtgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC          <  SRR4302532.1556198/85‑1 (MQ=60)
                                                                                 ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC          <  SRR4302532.4275069/85‑1 (MQ=60)
                                                                                 ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC          <  SRR4302532.3762560/85‑1 (MQ=60)
                                                                                 ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC          <  SRR4302532.1304730/85‑1 (MQ=60)
                                                                                 ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC          <  SRR4302532.84913/85‑1 (MQ=60)
                                                                                 ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC          <  SRR4302532.84912/85‑1 (MQ=60)
                                                                                  tgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCG         <  SRR4302532.2482929/86‑1 (MQ=60)
                                                                                   gcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGC        <  SRR4302532.5586855/87‑1 (MQ=60)
                                                                                   gcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGC        <  SRR4302532.2609899/87‑1 (MQ=60)
                                                                                   gcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAATATGATCGA        >  SRR4302532.1684198/8‑94 (MQ=60)
                                                                                    CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA       <  SRR4302532.1388138/94‑1 (MQ=60)
                                                                                     gcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAA      >  SRR4302532.61933/6‑94 (MQ=60)
                                                                                     gcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAA      >  SRR4302532.5384267/6‑94 (MQ=60)
                                                                                       ggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAAC    <  SRR4302532.4589337/91‑1 (MQ=60)
                                                                                       ggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAAC    >  SRR4302532.2924871/4‑94 (MQ=60)
                                                                                       C‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTGTCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG  >  SRR4302532.5607122/1‑94 (MQ=60)
                                                                                        gcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACT   <  SRR4302532.4685924/92‑1 (MQ=60)
                                                                                          |                                                                                            
CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG  >  NC_000913/4004900‑4005076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑