Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,006,483	+C	coding (183/207 nt)	b4554 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,006,480	1	.	C	98.5%	726.6 / 1.5	204	coding (186/207 nt)	b4554	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (114/87); total (117/87)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 221 total aligned reads displayed.

GCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006389‑4006567

gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑c                                                                                         <  2:2302841/93‑1 (MQ=255)

gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑c                                                                                         <  2:5356414/93‑1 (MQ=255)

  gcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccc                                                                                       <  2:3304621/93‑1 (MQ=255)

     ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaa                                                                                    <  2:4022865/93‑4 (MQ=255)

      cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaac                                                                                   >  2:5689633/1‑89 (MQ=255)

      cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaac                                                                                   >  1:73696/1‑89 (MQ=255)

      cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACGTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaac                                                                                   >  1:4560178/1‑89 (MQ=255)

        ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAccc                                                                                 <  1:4935043/93‑1 (MQ=255)

        ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAccc                                                                                 <  1:839972/93‑1 (MQ=255)

        ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑CAaccc                                                                                >  2:2328007/1‑93 (MQ=255)

        ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑CAaccc                                                                                >  2:1477029/1‑93 (MQ=255)

          gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCa                                                                               >  2:5576641/1‑93 (MQ=255)

          gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCa                                                                               <  1:4309714/93‑1 (MQ=255)

          gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCa                                                                               >  1:3183534/1‑93 (MQ=255)

             ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGc                                                                            >  1:4428693/1‑93 (MQ=255)

             ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGc                                                                            >  2:254386/1‑93 (MQ=255)

               aTTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCa                                                                          <  2:111957/93‑1 (MQ=255)

                ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCaa                                                                         >  1:2760036/1‑93 (MQ=255)

                   ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCa                                                                      <  2:537317/93‑1 (MQ=255)

                     aGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGc                                                                    >  1:5118161/1‑93 (MQ=255)

                       ccAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAACCCACGCCAACCAGcaa                                                                  >  1:3353793/1‑92 (MQ=255)

                        cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGcaca                                                                 <  1:4961092/93‑1 (MQ=255)

                         aaaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACaa                                                                >  2:1059472/1‑93 (MQ=255)

                          aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAat                                                               >  2:4457515/1‑93 (MQ=255)

                            tCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAatat                                                             >  1:3540198/1‑93 (MQ=255)

                              aaGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGa                                                           <  1:2426974/93‑1 (MQ=255)

                               aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGaa                                                          >  1:2177621/1‑93 (MQ=255)

                                 tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATc                                                        <  2:4881658/93‑1 (MQ=255)

                                   aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGTGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGc                                                      <  2:5656140/93‑1 (MQ=255)

                                   aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGc                                                      >  2:305532/1‑93 (MQ=255)

                                    gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGcc                                                     <  1:5450399/93‑1 (MQ=255)

                                     ccGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCa                                                    <  1:4057637/93‑1 (MQ=255)

                                       gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                  >  2:4712601/1‑93 (MQ=255)

                                       gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                  >  1:1394375/1‑93 (MQ=255)

                                        cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                 <  2:1994415/93‑1 (MQ=255)

                                         gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                <  1:5068422/93‑1 (MQ=255)

                                         gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  1:990656/1‑93 (MQ=255)

                                         gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  2:3585883/1‑93 (MQ=255)

                                          ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               >  2:4219120/1‑93 (MQ=255)

                                           cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              >  2:3451004/1‑93 (MQ=255)

                                           cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              >  1:6056973/1‑93 (MQ=255)

                                           cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCTAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              >  1:2279640/1‑93 (MQ=255)

                                            tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                             >  1:5515440/1‑93 (MQ=255)

                                             gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            >  1:2641335/1‑93 (MQ=255)

                                             gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            <  1:2118767/93‑1 (MQ=255)

                                             gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            >  1:4635155/1‑93 (MQ=255)

                                              cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           >  2:2941972/1‑93 (MQ=255)

                                              cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           <  2:3894208/93‑1 (MQ=255)

                                              cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           >  2:3074240/1‑93 (MQ=255)

                                               ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCta                                          >  1:1661383/1‑92 (MQ=255)

                                                cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         >  2:5226547/1‑93 (MQ=255)

                                                cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         <  2:762238/93‑1 (MQ=255)

                                                 acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        <  2:427191/93‑1 (MQ=255)

                                                 acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  2:1647996/1‑93 (MQ=255)

                                                 acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  1:1501612/1‑93 (MQ=255)

                                                 acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  2:3248584/1‑93 (MQ=255)

                                                  cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAg                                       >  2:1709788/1‑93 (MQ=255)

                                                   ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      >  2:2608016/1‑93 (MQ=255)

                                                   gtcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      <  2:2813882/92‑1 (MQ=255)

                                                    tcaacacatcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGcc                                     >  1:563667/1‑93 (MQ=255)

                                                     caacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                    <  2:4592599/93‑1 (MQ=255)

                                                     caacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                    <  2:5725716/93‑1 (MQ=255)

                                                      aacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATTAATCGCCAGTCCGGTCTGAAGCCCa                                   <  1:4223205/93‑1 (MQ=255)

                                                      aacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCa                                   <  2:5289873/93‑1 (MQ=255)

                                                      aacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCa                                   >  2:3786557/1‑93 (MQ=255)

                                                       acacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                  >  2:115953/1‑93 (MQ=255)

                                                       acacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                  >  2:5872681/1‑93 (MQ=255)

                                                         acttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                >  1:1585826/1‑93 (MQ=255)

                                                           ttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAt                              <  1:1222751/93‑1 (MQ=255)

                                                            tcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATa                             <  1:1064190/93‑1 (MQ=255)

                                                            tcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATa                             <  2:3952202/93‑1 (MQ=255)

                                                              aaaCGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           <  2:5410082/93‑1 (MQ=255)

                                                               aaCGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                          <  2:3676386/93‑1 (MQ=255)

                                                                 cGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGACCAGCAATAGACGc                        >  1:4764575/1‑93 (MQ=255)

                                                                  gCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       >  1:5309271/1‑93 (MQ=255)

                                                                   cAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCa                      >  1:3521385/1‑93 (MQ=255)

                                                                    aaTCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                     >  1:3869009/1‑93 (MQ=255)

                                                                    aaTCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                     <  1:5550612/93‑1 (MQ=255)

                                                                    aaTCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                     >  1:1183451/1‑93 (MQ=255)

                                                                     aTCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    <  2:3875728/93‑1 (MQ=255)

                                                                     aTCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    >  1:2449149/1‑93 (MQ=255)

                                                                     aTCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    >  2:6104170/1‑93 (MQ=255)

                                                                      tCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCAGGACTGACGCCCAGCAATAGACGacacac                   >  2:905926/1‑91 (MQ=255)

                                                                       cACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgc                  <  2:4904932/93‑1 (MQ=255)

                                                                       cACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgc                  <  2:2134524/93‑1 (MQ=255)

                                                                        aCTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcg                 >  2:2210082/1‑93 (MQ=255)

                                                                          tGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               <  2:1344917/93‑1 (MQ=255)

                                                                           gAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCACCCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg              <  2:4388995/93‑1 (MQ=255)

                                                                             gCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcg            >  1:3767794/1‑93 (MQ=255)

                                                                              cGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGa           <  1:2536080/93‑1 (MQ=255)

                                                                                ggAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCGGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         >  1:5149068/1‑93 (MQ=255)

                                                                                  aaaaTAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc       >  1:2911242/1‑93 (MQ=255)

                                                                                  aaaaTAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc       >  1:564373/1‑93 (MQ=255)

                                                                                   aaaTAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc      >  2:3989657/1‑93 (MQ=255)

                                                                                     aTAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    <  2:5559199/93‑1 (MQ=255)

                                                                                      tAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg   <  2:50657/93‑1 (MQ=255)

                                                                                      tAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg   <  1:1735864/93‑1 (MQ=255)

                                                                                      tAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCACCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg   >  1:1900482/1‑93 (MQ=255)

                                                                                      aaGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg   <  1:2036193/92‑1 (MQ=255)

                                                                                       aGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTgg  <  2:1414993/93‑1 (MQ=255)

GCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006389‑4006567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  >  NC_000913/4006390‑4006576

CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC                                                                                                >  SRR4302532.618800/1‑94 (MQ=60)

    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAC                                                                                            >  SRR4302532.76336/1‑94 (MQ=60)

    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAC                                                                                            >  SRR4302532.76337/1‑94 (MQ=60)

    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACGTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAC                                                                                            >  SRR4302532.4715737/1‑94 (MQ=60)

     CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACC                                                                                           >  SRR4302532.3881836/1‑94 (MQ=60)

       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                         <  SRR4302532.868793/94‑1 (MQ=60)

       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                         >  SRR4302532.5716019/1‑94 (MQ=60)

       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                         <  SRR4302532.5104487/94‑1 (MQ=60)

        TGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCA                                                                                        >  SRR4302532.4749163/1‑94 (MQ=60)

        TGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCA                                                                                        >  SRR4302532.3289245/1‑94 (MQ=60)

        TGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCA                                                                                        <  SRR4302532.4867516/94‑1 (MQ=60)

         GCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCAC                                                                                       <  SRR4302532.4455416/94‑1 (MQ=60)

           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCTAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                     >  SRR4302532.5667931/1‑94 (MQ=60)

           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAGTAGCGTCCCC‑AACCCCACAC                                                                                     >  SRR4302532.2934666/1‑94 (MQ=60)

           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                     >  SRR4302532.3354203/1‑94 (MQ=60)

           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                     >  SRR4302532.4579571/1‑94 (MQ=60)

            GGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCA                                                                                    >  SRR4302532.2236308/1‑94 (MQ=60)

              ATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAA                                                                                  >  SRR4302532.2852708/1‑94 (MQ=60)

               TTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAAC                                                                                 <  SRR4302532.5223132/94‑1 (MQ=60)

                 CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCA                                                                               >  SRR4302532.4592708/1‑94 (MQ=60)

                 CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCA                                                                               <  SRR4302532.3644760/94‑1 (MQ=60)

                  CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAG                                                                              <  SRR4302532.2603559/94‑1 (MQ=60)

                   CAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGC                                                                             >  SRR4302532.5294177/1‑94 (MQ=60)

                     GCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCAC                                                                           <  SRR4302532.5534312/94‑1 (MQ=60)

                     GCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAACCCACGCCAACCAGCAA                                                                           >  SRR4302532.3465759/1‑94 (MQ=60)

                      CCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACA                                                                          >  SRR4302532.2125997/1‑94 (MQ=60)

                       CAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAA                                                                         <  SRR4302532.5131248/94‑1 (MQ=60)

                         AATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATA                                                                       >  SRR4302532.3395731/1‑94 (MQ=60)

                         AATCAAGTAAGCCGCGCCTGCCCACTTCAACACCTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATA                                                                       <  SRR4302532.243750/94‑1 (MQ=60)

                          ATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATAT                                                                      >  SRR4302532.3658638/1‑94 (MQ=60)

                             AAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAA                                                                   >  SRR4302532.2250341/1‑94 (MQ=60)

                             AAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAA                                                                   <  SRR4302532.2507631/94‑1 (MQ=60)

                              AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAAT                                                                  <  SRR4302532.5412270/94‑1 (MQ=60)

                               GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATC                                                                 <  SRR4302532.5123915/94‑1 (MQ=60)

                               GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATAACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATC                                                                 >  SRR4302532.1275800/1‑94 (MQ=60)

                                TAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCG                                                                <  SRR4302532.258455/94‑1 (MQ=60)

                                  AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCC                                                              >  SRR4302532.177484/1‑94 (MQ=60)

                                   GCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCA                                                             <  SRR4302532.5639305/94‑1 (MQ=60)

                                    CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAG                                                            <  SRR4302532.2730585/94‑1 (MQ=60)

                                    CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAG                                                            <  SRR4302532.4194061/94‑1 (MQ=60)

                                     CGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                           >  SRR4302532.1441127/1‑94 (MQ=60)

                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                         >  SRR4302532.1024483/1‑94 (MQ=60)

                                        GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                        <  SRR4302532.5242563/94‑1 (MQ=60)

                                        CCCTGCCCACTTCACCACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                        <  SRR4302532.2518870/94‑1 (MQ=60)

                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                       <  SRR4302532.1542215/94‑1 (MQ=60)

                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                       >  SRR4302532.6266116/1‑94 (MQ=60)

                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCTAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                       >  SRR4302532.2355310/1‑94 (MQ=60)

                                          CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGT                                                      >  SRR4302532.5706839/1‑94 (MQ=60)

                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                     >  SRR4302532.4793201/1‑94 (MQ=60)

                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                     >  SRR4302532.5253842/1‑94 (MQ=60)

                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                     >  SRR4302532.2729910/1‑94 (MQ=60)

                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                     <  SRR4302532.1635250/94‑1 (MQ=60)

                                            GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                    <  SRR4302532.1607242/94‑1 (MQ=60)

                                            GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                    <  SRR4302532.2189552/94‑1 (MQ=60)

                                             CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTA                                                   >  SRR4302532.1717675/1‑94 (MQ=60)

                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                 >  SRR4302532.1552282/1‑94 (MQ=60)

                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                 >  SRR4302532.4516480/1‑94 (MQ=60)

                                                ACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAG                                                >  SRR4302532.4155537/1‑94 (MQ=60)

                                                 CTTCAACACTTCAAACGCAATAACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGCCTGAAGC                                               >  SRR4302532.3481397/1‑94 (MQ=60)

                                                  TTCAACACATCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACACTATGAATCGCCAGTCCGGTCTGAAGCC                                              >  SRR4302532.583330/1‑94 (MQ=60)

                                                  TTCAACACATCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCC                                              >  SRR4302532.583329/1‑94 (MQ=60)

                                                    CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                            >  SRR4302532.5297488/1‑94 (MQ=60)

                                                     AACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATTAATCGCCAGTCCGGTCTGAAGCCCAG                                           <  SRR4302532.4364930/94‑1 (MQ=60)

                                                     AACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAG                                           <  SRR4302532.3070351/94‑1 (MQ=60)

                                                       CACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCA                                         >  SRR4302532.2174174/1‑94 (MQ=60)

                                                       CACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCA                                         >  SRR4302532.1639498/1‑94 (MQ=60)

                                                          TTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATA                                      <  SRR4302532.1263650/94‑1 (MQ=60)

                                                           TCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAG                                     <  SRR4302532.5146105/94‑1 (MQ=60)

                                                           TCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAG                                     <  SRR4302532.1100001/94‑1 (MQ=60)

                                                             AAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                   <  SRR4302532.1690115/94‑1 (MQ=60)

                                                              AACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                  <  SRR4302532.5825886/94‑1 (MQ=60)

                                                              AACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                  >  SRR4302532.980690/1‑94 (MQ=60)

                                                              AACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                  >  SRR4302532.3778050/1‑94 (MQ=60)

                                                               ACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGC                                 <  SRR4302532.3949005/94‑1 (MQ=60)

                                                               ACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGACCAGCAATAGACGC                                 >  SRR4302532.4927851/1‑94 (MQ=60)

                                                                CGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCC                                >  SRR4302532.5492860/1‑94 (MQ=60)

                                                                 GCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCA                               >  SRR4302532.3639264/1‑94 (MQ=60)

                                                                  CAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAC                              >  SRR4302532.1222983/1‑94 (MQ=60)

                                                                  CAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAC                              >  SRR4302532.3999575/1‑94 (MQ=60)

                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                             <  SRR4302532.5743287/94‑1 (MQ=60)

                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                             >  SRR4302532.2530585/1‑94 (MQ=60)

                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                             <  SRR4302532.428165/94‑1 (MQ=60)

                                                                    ATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                            >  SRR4302532.5844084/1‑94 (MQ=60)

                                                                         TGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCG                       <  SRR4302532.11117/94‑1 (MQ=60)

                                                                         CGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCA                       <  SRR4302532.568946/94‑1 (MQ=60)

                                                                           AGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                     >  SRR4302532.2015494/1‑94 (MQ=60)

                                                                           AGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                     >  SRR4302532.3894595/1‑94 (MQ=60)

                                                                             CGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                   <  SRR4302532.2620691/94‑1 (MQ=60)

                                                                             CGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                   >  SRR4302532.2679784/1‑94 (MQ=60)

                                                                              GGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCGGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                  >  SRR4302532.5326200/1‑94 (MQ=60)

                                                                                GAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC                >  SRR4302532.3008306/1‑94 (MQ=60)

                                                                                GAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC                >  SRR4302532.584060/1‑94 (MQ=60)

                                                                                GAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC                >  SRR4302532.464151/1‑94 (MQ=60)

                                                                                 AAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACC               <  SRR4302532.3946200/94‑1 (MQ=60)

                                                                                   AATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGT             <  SRR4302532.4415146/94‑1 (MQ=60)

                                                                                   AATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGT             <  SRR4302532.1257832/94‑1 (MQ=60)

                                                                                   AATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGT             >  SRR4302532.6338415/1‑94 (MQ=60)

                                                                                    ATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG            <  SRR4302532.2257041/94‑1 (MQ=60)

                                                                                    ATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG            <  SRR4302532.1951878/94‑1 (MQ=60)

                                                                                    ATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCACCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG            >  SRR4302532.1963501/1‑94 (MQ=60)

                                                                                     aagcgtcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG           <  SRR4302532.2104017/87‑1 (MQ=60)

                                                                                     TAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG           <  SRR4302532.1794290/94‑1 (MQ=60)

                                                                                      AGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT          >  SRR4302532.713674/1‑94 (MQ=60)

                                                                                      AGCGTCCCC‑AACCCCAAGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAACCCAGCCATAGACGCCACCGCGCCGCGATAACCGTGGT          >  SRR4302532.6193299/1‑94 (MQ=60)

                                                                                        CGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTG        >  SRR4302532.5135474/1‑94 (MQ=60)

                                                                                         gtcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA       <  SRR4302532.4885255/91‑1 (MQ=60)

                                                                                          tcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAG      <  SRR4302532.3314411/92‑1 (MQ=60)

                                                                                          tcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAG      <  SRR4302532.5952091/92‑1 (MQ=60)

                                                                                          C‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGC     >  SRR4302532.499671/1‑94 (MQ=60)

                                                                                          C‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGC     >  SRR4302532.4463783/1‑94 (MQ=60)

                                                                                            CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG    >  SRR4302532.3638588/1‑94 (MQ=60)

                                                                                            CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG    >  SRR4302532.3439928/1‑94 (MQ=60)

                                                                                             CC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGA   >  SRR4302532.6199852/1‑94 (MQ=60)

                                                                                             CC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGA   >  SRR4302532.5039656/1‑94 (MQ=60)

                                                                                              C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  <  SRR4302532.656375/94‑1 (MQ=60)

                                                                                              C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  <  SRR4302532.5273192/94‑1 (MQ=60)

                                                                                              C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  >  SRR4302532.3016298/1‑94 (MQ=60)

CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  >  NC_000913/4006390‑4006576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`