Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,743	C→T	P101L (CCC→CTC)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,743	0	C	T	100.0%	936.4 / NA	255	P101L (CCC→CTC)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (111/144); total (111/144)

Only 100 of 262 total aligned reads displayed.

TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG  >  NC_000913/4016654‑4016832

gggtggCCCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaa                                                                                        <  1:5041449/92‑1 (MQ=255)

 ggtggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaaa                                                                                       >  1:3858545/1‑93 (MQ=255)

 ggtggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaaa                                                                                       >  1:168172/1‑93 (MQ=255)

  gtggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaaaa                                                                                      <  1:2068825/93‑1 (MQ=255)

  gtggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaaaa                                                                                      >  2:3899497/1‑93 (MQ=255)

   tggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAt                                                                                     <  2:5603395/93‑1 (MQ=255)

   tggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAt                                                                                     <  1:659376/93‑1 (MQ=255)

    ggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATg                                                                                    <  2:237805/93‑1 (MQ=255)

    ggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATg                                                                                    <  2:5014070/93‑1 (MQ=255)

       aCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                                                 <  1:5714259/93‑1 (MQ=255)

        ccGGCGCCCTGCCAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGTTCCTTGCTGATATCAATGAATCCATGCTCAAAGTGGGcc                                                                                <  1:500871/93‑1 (MQ=255)

         cGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcg                                                                               >  2:1157725/1‑93 (MQ=255)

          ggCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATTCTCAAAATGGGCcgc                                                                              >  1:4934068/1‑93 (MQ=255)

          ggCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgc                                                                              <  1:2567783/93‑1 (MQ=255)

           gCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgcg                                                                             >  1:1033214/1‑93 (MQ=255)

            cGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCga                                                                            <  1:1355480/93‑1 (MQ=255)

             gACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                                           >  2:1544121/1‑93 (MQ=255)

             gACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                                           >  1:3696822/1‑93 (MQ=255)

               ccTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGaa                                                                         <  2:3556137/93‑1 (MQ=255)

                cTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAg                                                                        >  1:2239486/1‑93 (MQ=255)

                 tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                                       >  2:5793043/1‑93 (MQ=255)

                    cAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGc                                                                    >  1:6066114/1‑93 (MQ=255)

                      gCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGt                                                                  >  1:2157498/1‑93 (MQ=255)

                       cGAGATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTa                                                                 <  2:475642/93‑1 (MQ=255)

                        gAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                                                <  2:5251248/93‑1 (MQ=255)

                         aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAat                                                               <  1:5170614/93‑1 (MQ=255)

                           aTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                                             >  1:4023320/1‑93 (MQ=255)

                           aTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                                             <  1:5851339/93‑1 (MQ=255)

                            ttCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATc                                                            <  1:970554/93‑1 (MQ=255)

                             tctcCCGCCTGGTCGGAGAAACTGGCAAAGTGTTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCAAGAAGCTGCGTAATATCg                                                           <  2:265927/93‑1 (MQ=255)

                             tctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCg                                                           <  2:4135886/93‑1 (MQ=255)

                             tctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCg                                                           <  1:4424746/93‑1 (MQ=255)

                             tctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCg                                                           <  2:6020657/93‑1 (MQ=255)

                              ttcCCGGCTGGTCGGAGAAACTGGCAAAGTGGTCCGTGCTGATATCAATGAATCCGTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                                          <  2:5187272/92‑1 (MQ=255)

                              ctcGCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                                          <  1:686943/93‑1 (MQ=255)

                              ctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                                          <  1:2496750/93‑1 (MQ=255)

                                cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                                        >  1:716027/1‑93 (MQ=255)

                                  cGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                                      >  2:3308319/1‑93 (MQ=255)

                                  cGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                                      <  1:5526298/93‑1 (MQ=255)

                                     cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                                   >  2:2099374/1‑93 (MQ=255)

                                     cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                                   >  1:4724556/1‑93 (MQ=255)

                                      tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                                  >  2:441281/1‑93 (MQ=255)

                                        gTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                                                >  1:1186078/1‑93 (MQ=255)

                                        gTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                                                <  2:3366766/93‑1 (MQ=255)

                                          cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTGATATCGGTGTGATTGGCaa                                              <  1:820657/93‑1 (MQ=255)

                                            gagaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACg                                            >  2:1815166/1‑93 (MQ=255)

                                             agaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                                           >  2:2496820/1‑93 (MQ=255)

                                              gaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                                          <  2:1996799/93‑1 (MQ=255)

                                              gaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                                          >  1:4247735/1‑93 (MQ=255)

                                              gaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                                          >  1:5427571/1‑93 (MQ=255)

                                                aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGGACTATCGGTGTGATTGGCAACGTTGa                                        >  1:4751653/1‑93 (MQ=255)

                                                  cTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGt                                      >  1:3529718/1‑93 (MQ=255)

                                                     gCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATg                                   <  1:1854299/93‑1 (MQ=255)

                                                       aaaGTGGTCCTTGCTAATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGtt                                 >  1:5341042/1‑93 (MQ=255)

                                                        aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTc                                <  2:3853066/93‑1 (MQ=255)

                                                         aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa                               <  1:1109748/93‑1 (MQ=255)

                                                         aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa                               <  1:2738564/93‑1 (MQ=255)

                                                         aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa                               <  2:5395805/93‑1 (MQ=255)

                                                          gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg                              >  2:5103514/1‑93 (MQ=255)

                                                          gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg                              >  2:4254652/1‑93 (MQ=255)

                                                          gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg                              >  2:1090477/1‑93 (MQ=255)

                                                          gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg                              >  2:164103/1‑93 (MQ=255)

                                                          gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg                              >  2:1606631/1‑93 (MQ=255)

                                                              tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGa                          >  2:2516139/1‑93 (MQ=255)

                                                              tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGa                          <  2:5573416/93‑1 (MQ=255)

                                                               ccTTGCTGAGATCAATGAATCCATGCTCAAACTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa                         <  2:477100/93‑1 (MQ=255)

                                                                cTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAc                        >  2:729025/1‑93 (MQ=255)

                                                                 ttGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg                       >  2:800455/1‑93 (MQ=255)

                                                                   gCTGATATCAATGAATCCATGCTAAAAATGGGCCGCGAGAAGCTGCGTAATATCGATGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCt                     <  1:1162025/93‑1 (MQ=255)

                                                                     tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                   <  2:1297938/93‑1 (MQ=255)

                                                                     tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                   >  2:5897238/1‑93 (MQ=255)

                                                                     tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                   <  2:4997241/93‑1 (MQ=255)

                                                                     tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                   >  1:3106509/1‑93 (MQ=255)

                                                                      gATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAg                  <  2:3219376/93‑1 (MQ=255)

                                                                      gATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAg                  >  1:3348906/1‑93 (MQ=255)

                                                                       atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAgg                 <  1:4699771/93‑1 (MQ=255)

                                                                       atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAgg                 <  1:2704229/93‑1 (MQ=255)

                                                                       atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAgg                 <  1:3152037/93‑1 (MQ=255)

                                                                        tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGgc                >  1:1487011/1‑93 (MQ=255)

                                                                          tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGgcgc              >  1:1778009/1‑93 (MQ=255)

                                                                          tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGgcgc              >  2:891832/1‑93 (MQ=255)

                                                                           cAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCt             >  2:2975733/1‑93 (MQ=255)

                                                                            aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTg            >  1:1196233/1‑93 (MQ=255)

                                                                             aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGc           >  2:2206865/1‑93 (MQ=255)

                                                                             aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGc           >  2:1831021/1‑93 (MQ=255)

                                                                             aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGc           >  2:781579/1‑93 (MQ=255)

                                                                             aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGc           >  2:1263147/1‑93 (MQ=255)

                                                                              tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGcc          >  1:4833687/1‑93 (MQ=255)

                                                                              tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGcc          >  2:4808473/1‑93 (MQ=255)

                                                                              tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGcc          <  2:3757132/93‑1 (MQ=255)

                                                                                aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGt        >  1:235412/1‑93 (MQ=255)

                                                                                 aTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGtt       >  1:1592735/1‑93 (MQ=255)

                                                                                  tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc      >  2:5441307/1‑93 (MQ=255)

                                                                                  tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc      <  1:3429454/93‑1 (MQ=255)

                                                                                   ccATGCTCAACATGGGCCGCGAGAATCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGTCGAACTCTGAGGCGCTACCGTTcc     <  1:3178879/93‑1 (MQ=255)

                                                                                    cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    >  1:1986418/1‑93 (MQ=255)

                                                                                    cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    <  2:6138583/93‑1 (MQ=255)

                                                                                    cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    >  2:4581923/1‑93 (MQ=255)

                                                                                     aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCg   <  2:5913034/93‑1 (MQ=255)

                                                                                      tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCgg  >  2:2795806/1‑93 (MQ=255)

TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG  >  NC_000913/4016654‑4016832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATA  >  NC_000913/4016650‑4016835

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                              >  SRR4302532.2836846/1‑94 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                              >  SRR4302532.4760052/1‑94 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                              <  SRR4302532.5056591/94‑1 (MQ=60)

 GGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTC                                                                                             >  SRR4302532.2238689/1‑94 (MQ=60)

    TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAA                                                                                          >  SRR4302532.173805/1‑94 (MQ=60)

    TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAA                                                                                          >  SRR4302532.3988820/1‑94 (MQ=60)

    GGGTGGCCCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAA                                                                                          <  SRR4302532.5214393/94‑1 (MQ=60)

      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                        <  SRR4302532.2137764/94‑1 (MQ=60)

      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                        <  SRR4302532.724059/94‑1 (MQ=60)

      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                        <  SRR4302532.4019908/94‑1 (MQ=60)

       TGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATG                                                                                       <  SRR4302532.1480086/94‑1 (MQ=60)

       TGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATG                                                                                       <  SRR4302532.682197/94‑1 (MQ=60)

         GCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGG                                                                                     <  SRR4302532.2320409/94‑1 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                   <  SRR4302532.5913376/94‑1 (MQ=60)

            CCGGCGCCCTGCCAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGTTCCTTGCTGATATCAATGAATCCATGCTCAAAGTGGGCCG                                                                                  <  SRR4302532.518390/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                  <  SRR4302532.518389/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                  >  SRR4302532.4863409/1‑94 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                  <  SRR4302532.1226849/94‑1 (MQ=60)

             CGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATTCTCAAAATGGGCCGC                                                                                 >  SRR4302532.5103492/1‑94 (MQ=60)

              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                >  SRR4302532.1068106/1‑94 (MQ=60)

              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                <  SRR4302532.2653495/94‑1 (MQ=60)

              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                <  SRR4302532.6149346/94‑1 (MQ=60)

                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAG                                                                              >  SRR4302532.3820936/1‑94 (MQ=60)

                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAG                                                                              <  SRR4302532.1400834/94‑1 (MQ=60)

                 GACCTGACAGCGAAATTGTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                             >  SRR4302532.4307604/1‑94 (MQ=60)

                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                             >  SRR4302532.3040136/1‑94 (MQ=60)

                  ACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAA                                                                            <  SRR4302532.1110008/94‑1 (MQ=60)

                   CCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAG                                                                           <  SRR4302532.6291974/94‑1 (MQ=60)

                   CCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAG                                                                           <  SRR4302532.879274/94‑1 (MQ=60)

                   CCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAG                                                                           >  SRR4302532.2313798/1‑94 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                         <  SRR4302532.4882777/94‑1 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                        <  SRR4302532.116225/94‑1 (MQ=60)

                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                       >  SRR4302532.6275617/1‑94 (MQ=60)

                         AGCGAAATTCTCCCGCCTGGTCGGAGTAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                     >  SRR4302532.5665140/1‑94 (MQ=60)

                         AGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGcagaggcgt                                                                     >  SRR4302532.1584741/1‑85 (MQ=60)

                         AGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                     >  SRR4302532.2229629/1‑94 (MQ=60)

                             AAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATA                                                                 <  SRR4302532.5348694/94‑1 (MQ=60)

                             AAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATA                                                                 <  SRR4302532.721936/94‑1 (MQ=60)

                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                <  SRR4302532.850155/94‑1 (MQ=60)

                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                >  SRR4302532.4158902/1‑94 (MQ=60)

                               ATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATC                                                               <  SRR4302532.6054318/94‑1 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                              <  SRR4302532.1003664/94‑1 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                              <  SRR4302532.4892851/94‑1 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                              >  SRR4302532.2687088/1‑94 (MQ=60)

                                 TCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGG                                                             <  SRR4302532.4575489/94‑1 (MQ=60)

                                 TCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAAT‑CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGT                                                            <  SRR4302532.2167785/94‑1 (MQ=60)

                                  CTCGCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGT                                                            <  SRR4302532.710507/94‑1 (MQ=60)

                                  CTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGT                                                            <  SRR4302532.2580001/94‑1 (MQ=60)

                                  CTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGT                                                            <  SRR4302532.4528139/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                           >  SRR4302532.740486/1‑94 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                           >  SRR4302532.3436974/1‑94 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                           <  SRR4302532.1399936/94‑1 (MQ=60)

                                   CCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                           <  SRR4302532.1683539/94‑1 (MQ=60)

                                    CCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGT                                                          <  SRR4302532.3716185/94‑1 (MQ=60)

                                      CGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGA                                                        <  SRR4302532.5718067/94‑1 (MQ=60)

                                        GCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                      <  SRR4302532.5082373/94‑1 (MQ=60)

                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                      >  SRR4302532.4885776/1‑94 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                     >  SRR4302532.4013565/1‑94 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                     <  SRR4302532.5443445/94‑1 (MQ=60)

                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGC                                                   >  SRR4302532.1225721/1‑94 (MQ=60)

                                            GTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCA                                                  <  SRR4302532.4954895/94‑1 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTGATATCGGTGTGATTGGCAAC                                                <  SRR4302532.848659/94‑1 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                >  SRR4302532.3273245/1‑94 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                               >  SRR4302532.4848298/1‑94 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                               <  SRR4302532.2683674/94‑1 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                               >  SRR4302532.2251865/1‑94 (MQ=60)

                                                GAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGT                                              <  SRR4302532.550308/94‑1 (MQ=60)

                                                GAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGT                                              >  SRR4302532.1611859/1‑94 (MQ=60)

                                                 AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTT                                             >  SRR4302532.5615677/1‑94 (MQ=60)

                                                 AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTT                                             >  SRR4302532.4390454/1‑94 (MQ=60)

                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGGACTATCGGTGTGATTGGCAACGTTGA                                           >  SRR4302532.4914116/1‑94 (MQ=60)

                                                    AACTGGCAAAGTGTTCCTTGATGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAG                                          <  SRR4302532.2209773/94‑1 (MQ=60)

                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGT                                         <  SRR4302532.4905475/94‑1 (MQ=60)

                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGT                                         >  SRR4302532.3647852/1‑94 (MQ=60)

                                                       TGGCAAAGTGGTCCTTGCTGATAGCAATGAATCCATCCTCAAAATGGCCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAT                                       <  SRR4302532.814630/94‑1 (MQ=60)

                                                       CGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAT                                       <  SRR4302532.2615367/94‑1 (MQ=60)

                                                        GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATG                                      <  SRR4302532.260967/94‑1 (MQ=60)

                                                        GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATG                                      <  SRR4302532.1752920/94‑1 (MQ=60)

                                                         GCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGT                                     <  SRR4302532.1915810/94‑1 (MQ=60)

                                                          CAAAGTGGTCCTTGCTAATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTT                                    >  SRR4302532.5526327/1‑94 (MQ=60)

                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                 <  SRR4302532.2830595/94‑1 (MQ=60)

                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                 <  SRR4302532.1146850/94‑1 (MQ=60)

                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                 <  SRR4302532.705512/94‑1 (MQ=60)

                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                 >  SRR4302532.3491609/1‑94 (MQ=60)

                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                 <  SRR4302532.705511/94‑1 (MQ=60)

                                                              GTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAaggcacgcggaaacgtgcggatacgcgggtggattgcaaacctgagtgatgtatagg                                >  SRR4302532.4542022/1‑37 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                              <  SRR4302532.5858286/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                              <  SRR4302532.1346775/94‑1 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGCAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                          >  SRR4302532.4967068/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                          >  SRR4302532.5577171/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                          <  SRR4302532.2076591/94‑1 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                          <  SRR4302532.3551437/94‑1 (MQ=60)

                                                                     TTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGC                         <  SRR4302532.4842827/94‑1 (MQ=60)

                                                                     TTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGATGAGTATGTTCAGACGAACGT                         >  SRR4302532.197693/1‑94 (MQ=60)

                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                        <  SRR4302532.3803632/94‑1 (MQ=60)

                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                        <  SRR4302532.6022524/94‑1 (MQ=60)

                                                                       GCTGATATCAATGAATCCATGCTAAAAATGGGCCGCGAGAAGCTGCGTAATATCGATGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTG                       <  SRR4302532.1200846/94‑1 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                      >  SRR4302532.3209543/1‑94 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAG                     >  SRR4302532.3460714/1‑94 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAG                     <  SRR4302532.3546085/94‑1 (MQ=60)

                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGG                    >  SRR4302532.3368012/1‑94 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGG                   >  SRR4302532.5185170/1‑94 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                   >  SRR4302532.1537178/1‑94 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                   <  SRR4302532.2795114/94‑1 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                   <  SRR4302532.3256634/94‑1 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                   <  SRR4302532.3256635/94‑1 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                   <  SRR4302532.4860088/94‑1 (MQ=60)

                                                                            TATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCG                  <  SRR4302532.1201945/94‑1 (MQ=60)

                                                                            TATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCG                  <  SRR4302532.1872336/94‑1 (MQ=60)

                                                                            TATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGATCAGGCGAACGCTGAGGCG                  <  SRR4302532.1872337/94‑1 (MQ=60)

                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGC                 >  SRR4302532.1837508/1‑94 (MQ=60)

                                                                              TCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCT                <  SRR4302532.5242166/94‑1 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGGGCTG               >  SRR4302532.1236227/1‑94 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG               >  SRR4302532.1236228/1‑94 (MQ=60)

                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCC             >  SRR4302532.5000068/1‑94 (MQ=60)

                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGT           >  SRR4302532.243493/1‑94 (MQ=60)

                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGT           >  SRR4302532.243492/1‑94 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT          <  SRR4302532.4575401/94‑1 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT          <  SRR4302532.245404/94‑1 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT          >  SRR4302532.1646649/1‑94 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT          <  SRR4302532.2795919/94‑1 (MQ=60)

                                                                                      TCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCC        >  SRR4302532.5308693/1‑94 (MQ=60)

                                                                                      TCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCC        <  SRR4302532.6259882/94‑1 (MQ=60)

                                                                                      TCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCC        <  SRR4302532.3544357/94‑1 (MQ=60)

                                                                                       CCATGCTCAACATGGGCCGCGAGAATCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGTCGAACTCTGAGGCGCTACCGTTCCC       <  SRR4302532.3284440/94‑1 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC       >  SRR4302532.5881293/1‑94 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC       >  SRR4302532.2052418/1‑94 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC       <  SRR4302532.3849192/94‑1 (MQ=60)

                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG      >  SRR4302532.3238286/1‑94 (MQ=60)

                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG      <  SRR4302532.1832620/94‑1 (MQ=60)

                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG      <  SRR4302532.210890/94‑1 (MQ=60)

                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG     >  SRR4302532.1189235/1‑94 (MQ=60)

                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG     >  SRR4302532.2999091/1‑94 (MQ=60)

                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG     >  SRR4302532.5150044/1‑94 (MQ=60)

                                                                                          TGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGA    >  SRR4302532.2195255/1‑94 (MQ=60)

                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT   <  SRR4302532.3534520/94‑1 (MQ=60)

                                                                                            CTCAAAATGGGCCGCGAGAAGCTGCGTAATACCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATA  >  SRR4302532.5536451/1‑94 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATA  >  NC_000913/4016650‑4016835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`