Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,039,960 Δ1 bp intergenic (+42/‑36) yihD → / → yihE hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,039,9590A.99.6% 1137.6 / ‑2.5 243intergenic (+41/‑37)yihD/yihEhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (135/107);  total (136/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 275 total aligned reads displayed.

AGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGG  >  NC_000913/4039867‑4040047
                                                                                            |                                                                                        
agaagaCTTCAAAACGGCGCTGTTACGCGTTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAAACATCGTGAAATGATa                                                                                          <  2:3812582/93‑1 (MQ=255)
agaagaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATa                                                                                          <  2:3596660/93‑1 (MQ=255)
agaagaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATa                                                                                          <  1:2979823/93‑1 (MQ=255)
 gaagaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATat                                                                                         >  1:5813811/1‑92 (MQ=255)
  aagaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATatc                                                                                        <  2:856883/93‑3 (MQ=255)
  aagaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGGAAAAGCTTGTAAGCGGCGCCACCAAAATCCTCGTGAAATGATatc                                                                                        >  1:2692169/1‑91 (MQ=255)
  aagaCTTCAAAACGGCGCTGTTACGCGCTCGCCGCGTAATTAAAGAGTAAAACCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATatc                                                                                        <  2:1258669/93‑3 (MQ=255)
   agaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATatcc                                                                                       >  2:5081459/1‑90 (MQ=255)
   agaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATatcc                                                                                       <  1:3840242/93‑4 (MQ=255)
    gaCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATatcct                                                                                      >  2:3736084/1‑89 (MQ=255)
     aCTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCtt                                                                                    >  2:878564/1‑93 (MQ=255)
      cTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTc                                                                                   <  2:2600570/93‑1 (MQ=255)
      cTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTc                                                                                   <  1:980793/93‑1 (MQ=255)
       ttCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCg                                                                                  >  1:1922468/1‑93 (MQ=255)
         cAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTc                                                                                >  1:5357483/1‑93 (MQ=255)
         cAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTc                                                                                >  1:5558709/1‑93 (MQ=255)
          aaaaCGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCa                                                                               >  1:5160589/1‑93 (MQ=255)
             aCGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTc                                                                            >  2:4788599/1‑93 (MQ=255)
               ggCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGt                                                                          <  2:6111204/93‑1 (MQ=255)
                gcgcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTa                                                                         <  1:72599/93‑1 (MQ=255)
                gagcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTa                                                                         <  1:3344335/91‑1 (MQ=255)
                  gcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAAt                                                                       >  1:1543592/1‑93 (MQ=255)
                  gcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAAt                                                                       >  2:5705002/1‑93 (MQ=255)
                   cTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATg                                                                      >  1:5418051/1‑93 (MQ=255)
                    tGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGt                                                                     >  2:5405115/1‑93 (MQ=255)
                     gTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtt                                                                    >  1:2225341/1‑93 (MQ=255)
                     gTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCCTGAAATGAT‑ATCCTTCGTCATTCGTAATGtt                                                                    >  1:5509269/1‑93 (MQ=255)
                       tACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtttt                                                                  <  2:4818218/93‑1 (MQ=255)
                       tACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtttt                                                                  <  1:2392000/93‑1 (MQ=255)
                          gcgcTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCg                                                               <  2:3015054/93‑1 (MQ=255)
                            gcTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGga                                                             <  1:2886602/93‑1 (MQ=255)
                            gcTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGga                                                             <  1:1449650/93‑1 (MQ=255)
                             cTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgat                                                            >  2:4046240/1‑93 (MQ=255)
                               cgcgGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgatga                                                          >  2:746516/1‑93 (MQ=255)
                               cgcgGCGTAATTAAAGAGTAAAAGCGTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTACCGgatga                                                          <  2:1870896/93‑1 (MQ=255)
                                 cggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGatgatg                                                        <  1:834573/93‑1 (MQ=255)
                                   gcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATggg                                                      >  2:3122279/1‑93 (MQ=255)
                                    cgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGa                                                     <  1:4240779/93‑1 (MQ=255)
                                    cgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGa                                                     <  2:4696003/93‑1 (MQ=255)
                                     gTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAt                                                    >  2:3675925/1‑93 (MQ=255)
                                     gTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAt                                                    <  2:5021227/93‑1 (MQ=255)
                                      tAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATg                                                   >  1:4592019/1‑93 (MQ=255)
                                      tAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATg                                                   >  2:1768892/1‑93 (MQ=255)
                                      tAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATg                                                   >  2:1085295/1‑93 (MQ=255)
                                         ttAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAt                                                >  1:1440994/1‑93 (MQ=255)
                                         ttAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAt                                                >  2:2681183/1‑93 (MQ=255)
                                         ttAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAt                                                >  2:1581520/1‑93 (MQ=255)
                                          tAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATa                                               <  1:5935930/93‑1 (MQ=255)
                                          tAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATa                                               <  2:4714584/93‑1 (MQ=255)
                                           aaaGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATaa                                              >  1:2165068/1‑93 (MQ=255)
                                            aaGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAAc                                             >  2:5129045/1‑93 (MQ=255)
                                              gagTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAg                                           <  2:3274771/93‑1 (MQ=255)
                                              gagTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAg                                           <  1:558150/93‑1 (MQ=255)
                                               agTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgc                                          <  2:1429328/93‑1 (MQ=255)
                                                gTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcg                                         <  2:4154518/93‑1 (MQ=255)
                                                 tAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcgc                                        <  1:320464/93‑1 (MQ=255)
                                                 tAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcgc                                        <  1:5318558/93‑1 (MQ=255)
                                                      gCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa                                   <  1:5181092/93‑1 (MQ=255)
                                                       cTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGTATAACAGCGCTTTTAc                                  >  2:5498864/1‑93 (MQ=255)
                                                       cTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAc                                  >  1:3039416/1‑93 (MQ=255)
                                                       cTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAc                                  >  2:221603/1‑93 (MQ=255)
                                                        ttGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACt                                 >  2:5282983/1‑93 (MQ=255)
                                                         tGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACtt                                <  2:3447356/93‑1 (MQ=255)
                                                         tGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACtt                                >  2:4391625/1‑93 (MQ=255)
                                                          gTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACttt                               >  2:5866791/1‑93 (MQ=255)
                                                          gTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACttt                               <  1:355180/93‑1 (MQ=255)
                                                          gTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACttt                               <  1:4440820/93‑1 (MQ=255)
                                                           tAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTc                              <  2:5962649/93‑1 (MQ=255)
                                                            aaGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTcc                             <  2:1372897/93‑1 (MQ=255)
                                                              gcggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAg                           <  2:6025002/93‑1 (MQ=255)
                                                               cggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGa                          <  2:205620/93‑1 (MQ=255)
                                                               cggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGa                          <  1:1369606/93‑1 (MQ=255)
                                                                ggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAGTAACAGCGCTTTTACTTTCCAGac                         >  2:447335/1‑93 (MQ=255)
                                                                ggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCCGaa                         >  1:656545/1‑92 (MQ=255)
                                                                 gcgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGaca                        >  1:1025976/1‑93 (MQ=255)
                                                                 gcgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGaca                        >  1:4024010/1‑93 (MQ=255)
                                                                  cgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGagac                       <  1:4952953/93‑1 (MQ=255)
                                                                  cgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGacac                       <  2:4189043/93‑1 (MQ=255)
                                                                   gcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACt                      >  1:2736006/1‑93 (MQ=255)
                                                                    ctaccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTa                     <  1:5854963/91‑1 (MQ=255)
                                                                     caccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTac                    <  2:5909715/93‑1 (MQ=255)
                                                                     caccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTac                    >  2:4110630/1‑93 (MQ=255)
                                                                     caccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTac                    >  1:1205556/1‑93 (MQ=255)
                                                                       ccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTacac                  >  1:206289/1‑93 (MQ=255)
                                                                        caAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc                 <  1:774010/93‑1 (MQ=255)
                                                                        caAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc                 <  2:1466648/93‑1 (MQ=255)
                                                                        caAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc                 <  2:704488/93‑1 (MQ=255)
                                                                        caAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc                 >  2:3058986/1‑93 (MQ=255)
                                                                            atcatcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGa             >  1:275383/1‑93 (MQ=255)
                                                                            atcatcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGa             <  1:4121954/93‑1 (MQ=255)
                                                                                tcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATAcc         >  1:3795480/1‑93 (MQ=255)
                                                                                tcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATAcc         >  1:1875962/1‑93 (MQ=255)
                                                                                 cGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACca        <  2:189406/93‑1 (MQ=255)
                                                                                  gTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcat       <  2:2332222/93‑1 (MQ=255)
                                                                                   tGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcatc      >  2:3209909/1‑93 (MQ=255)
                                                                                   tGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcatc      <  1:105052/93‑1 (MQ=255)
                                                                                    gAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcatca     <  2:2388980/93‑1 (MQ=255)
                                                                                    gAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcatca     >  2:1169061/1‑93 (MQ=255)
                                                                                     aaaTGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcatcat    >  1:1962544/1‑93 (MQ=255)
                                                                                     aaaTGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACcatcat    >  1:1765629/1‑93 (MQ=255)
                                                                                            |                                                                                        
AGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGG  >  NC_000913/4039867‑4040047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTC  >  NC_000913/4039867‑4040053
                                                                                             |                                                                                             
AGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAT                                                                                               <  SRR4302532.3078385/94‑1 (MQ=60)
AGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAT                                                                                               >  SRR4302532.6015771/1‑94 (MQ=60)
  AAGACTTCAAAACGGGTCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCC                                                                                            <  SRR4302532.6047573/94‑1 (MQ=60)
  AAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCC                                                                                            >  SRR4302532.2785643/1‑94 (MQ=60)
  AAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCC                                                                                            <  SRR4302532.2241980/94‑1 (MQ=60)
  AAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCC                                                                                            >  SRR4302532.147387/1‑94 (MQ=60)
   AGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCT                                                                                           <  SRR4302532.3969897/94‑1 (MQ=60)
   AGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCT                                                                                           <  SRR4302532.5543286/94‑1 (MQ=60)
   AGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCT                                                                                           >  SRR4302532.5769206/1‑94 (MQ=60)
    GACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTT                                                                                          <  SRR4302532.5748422/94‑1 (MQ=60)
     ACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTC                                                                                         <  SRR4302532.2050009/94‑1 (MQ=60)
      CTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCG                                                                                        <  SRR4302532.1014285/94‑1 (MQ=60)
      CTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCG                                                                                        >  SRR4302532.1986414/1‑94 (MQ=60)
      CGTCAAAACGGCGCTGTGACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCATGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAA‑CCTTCG                                                                                        >  SRR4302532.5688373/1‑94 (MQ=60)
       TTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGT                                                                                       <  SRR4302532.4251590/94‑1 (MQ=60)
        TCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTC                                                                                      >  SRR4302532.5751714/1‑94 (MQ=60)
        TCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTC                                                                                      >  SRR4302532.5543251/1‑94 (MQ=60)
        TCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTC                                                                                      <  SRR4302532.6135973/94‑1 (MQ=60)
         CAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCT                                                                                     >  SRR4302532.1993990/1‑94 (MQ=60)
         CAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCA                                                                                     >  SRR4302532.4848290/1‑94 (MQ=60)
         CAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCA                                                                                     >  SRR4302532.5338191/1‑94 (MQ=60)
         CAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCA                                                                                     >  SRR4302532.2686989/1‑94 (MQ=60)
         CAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCA                                                                                     >  SRR4302532.1993991/1‑94 (MQ=60)
          AAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAATTGAT‑ATCCTTCGTCAT                                                                                    >  SRR4302532.1895634/1‑94 (MQ=60)
          AAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCAT                                                                                    >  SRR4302532.5362665/1‑94 (MQ=60)
          AAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCAT                                                                                    >  SRR4302532.859150/1‑94 (MQ=60)
             ACGGGTCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCG                                                                                 <  SRR4302532.2299395/94‑1 (MQ=60)
             ACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCG                                                                                 <  SRR4302532.696542/94‑1 (MQ=60)
                GCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAA                                                                              <  SRR4302532.75192/94‑1 (MQ=60)
                GAGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAA                                                                              <  SRR4302532.3455955/94‑1 (MQ=60)
                 CGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAAT                                                                             >  SRR4302532.1595748/1‑94 (MQ=60)
                  GCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATG                                                                            >  SRR4302532.5605834/1‑94 (MQ=60)
                  GCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATG                                                                            >  SRR4302532.306224/1‑94 (MQ=60)
                  GCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATG                                                                            >  SRR4302532.5465787/1‑94 (MQ=60)
                    TGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTT                                                                          >  SRR4302532.2299197/1‑94 (MQ=60)
                    TGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTT                                                                          >  SRR4302532.2299199/1‑94 (MQ=60)
                    TGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTT                                                                          >  SRR4302532.5268929/1‑94 (MQ=60)
                    TGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTT                                                                          >  SRR4302532.6153822/1‑94 (MQ=60)
                    TGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCCTGAAATGAT‑ATCCTTCGTCATTCGTAATGTT                                                                          >  SRR4302532.5700412/1‑94 (MQ=60)
                     GTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTCATGTTT                                                                         >  SRR4302532.4167151/1‑94 (MQ=60)
                     GTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTT                                                                         <  SRR4302532.1178522/94‑1 (MQ=60)
                       TACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTC                                                                       <  SRR4302532.5755172/94‑1 (MQ=60)
                       TACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTC                                                                       <  SRR4302532.2471684/94‑1 (MQ=60)
                        ACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCC                                                                      >  SRR4302532.2882693/1‑94 (MQ=60)
                         CGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCG                                                                     <  SRR4302532.706864/94‑1 (MQ=60)
                         CGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCG                                                                     <  SRR4302532.3116285/94‑1 (MQ=60)
                          GCGCTCGCGTCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGG                                                                    <  SRR4302532.3786318/94‑1 (MQ=60)
                            GCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGAT                                                                  <  SRR4302532.1498523/94‑1 (MQ=60)
                            GCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGAT                                                                  <  SRR4302532.2983049/94‑1 (MQ=60)
                             CTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATG                                                                 >  SRR4302532.2628294/1‑94 (MQ=60)
                             CTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATG                                                                 <  SRR4302532.4109195/94‑1 (MQ=60)
                               CGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGAT                                                               >  SRR4302532.222657/1‑94 (MQ=60)
                                 CGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGG                                                             >  SRR4302532.338595/1‑94 (MQ=60)
                                 CGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGG                                                             >  SRR4302532.917139/1‑94 (MQ=60)
                                 CGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGG                                                             <  SRR4302532.863193/94‑1 (MQ=60)
                                    CGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAT                                                          <  SRR4302532.4383242/94‑1 (MQ=60)
                                    CGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAT                                                          >  SRR4302532.794638/1‑94 (MQ=60)
                                    CGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAT                                                          >  SRR4302532.3501725/1‑94 (MQ=60)
                                    CGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAT                                                          >  SRR4302532.1269476/1‑94 (MQ=60)
                                     GTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATG                                                         >  SRR4302532.907369/1‑94 (MQ=60)
                                     GTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATG                                                         >  SRR4302532.4748566/1‑94 (MQ=60)
                                      TAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCATGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGA                                                        >  SRR4302532.4650989/1‑94 (MQ=60)
                                        ATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAT                                                      <  SRR4302532.5342115/94‑1 (MQ=60)
                                        ATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAT                                                      >  SRR4302532.1489567/1‑94 (MQ=60)
                                        ATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAT                                                      >  SRR4302532.1489566/1‑94 (MQ=60)
                                          TAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCTTAATGTTTTCCGGATGATGGGATGAATAA                                                    <  SRR4302532.4152613/94‑1 (MQ=60)
                                          TAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAA                                                    <  SRR4302532.6141356/94‑1 (MQ=60)
                                          TAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAA                                                    >  SRR4302532.2237481/1‑94 (MQ=60)
                                          TAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAA                                                    <  SRR4302532.5524650/94‑1 (MQ=60)
                                            AAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACA                                                  >  SRR4302532.4519710/1‑94 (MQ=60)
                                             AGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAG                                                 <  SRR4302532.2818909/94‑1 (MQ=60)
                                             AGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAG                                                 <  SRR4302532.6004892/94‑1 (MQ=60)
                                              GAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGC                                                <  SRR4302532.577614/94‑1 (MQ=60)
                                               AGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCG                                               <  SRR4302532.4746276/94‑1 (MQ=60)
                                                 TAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCT                                             <  SRR4302532.331592/94‑1 (MQ=60)
                                                 TAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCT                                             <  SRR4302532.5502641/94‑1 (MQ=60)
                                                      GCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAC                                        >  SRR4302532.3139696/1‑94 (MQ=60)
                                                      GCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAC                                        >  SRR4302532.1818194/1‑94 (MQ=60)
                                                      GCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAC                                        <  SRR4302532.1569386/94‑1 (MQ=60)
                                                      GCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAC                                        <  SRR4302532.5359539/94‑1 (MQ=60)
                                                       CTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACT                                       >  SRR4302532.1445492/1‑94 (MQ=60)
                                                       CTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACT                                       >  SRR4302532.1973971/1‑94 (MQ=60)
                                                        TTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTT                                      >  SRR4302532.852840/1‑94 (MQ=60)
                                                          GTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTC                                    <  SRR4302532.4592139/94‑1 (MQ=60)
                                                          GTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTC                                    <  SRR4302532.367473/94‑1 (MQ=60)
                                                            AAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCA                                  <  SRR4302532.3820237/94‑1 (MQ=60)
                                                             AGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAG                                 >  SRR4302532.3925433/1‑94 (MQ=60)
                                                              GCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGA                                <  SRR4302532.1660112/94‑1 (MQ=60)
                                                              GCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGA                                >  SRR4302532.3117584/1‑94 (MQ=60)
                                                               CGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCcgaa                               >  SRR4302532.679298/1‑90 (MQ=60)
                                                               CGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGAC                               <  SRR4302532.1415485/94‑1 (MQ=60)
                                                                GGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACA                              >  SRR4302532.1060691/1‑94 (MQ=60)
                                                                GGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACA                              >  SRR4302532.5065805/1‑94 (MQ=60)
                                                                GGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACA                              >  SRR4302532.4159615/1‑94 (MQ=60)
                                                                  CGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGAGACT                            <  SRR4302532.5122886/94‑1 (MQ=60)
                                                                  CGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACT                            >  SRR4302532.1582307/1‑94 (MQ=60)
                                                                  CGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACT                            >  SRR4302532.5923967/1‑94 (MQ=60)
                                                                  CGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACT                            >  SRR4302532.2827960/1‑94 (MQ=60)
                                                                    CTACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTAC                          <  SRR4302532.6058010/94‑1 (MQ=60)
                                                                    CCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTAC                          >  SRR4302532.1245894/1‑94 (MQ=60)
                                                                    CCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTAC                          >  SRR4302532.1334874/1‑94 (MQ=60)
                                                                     CACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACA                         <  SRR4302532.4352151/94‑1 (MQ=60)
                                                                     CACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACA                         >  SRR4302532.3556080/1‑94 (MQ=60)
                                                                     CACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACA                         >  SRR4302532.3268377/1‑94 (MQ=60)
                                                                      ACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAC                        >  SRR4302532.213336/1‑94 (MQ=60)
                                                                      ACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAC                        >  SRR4302532.213335/1‑94 (MQ=60)
                                                                      ACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAC                        >  SRR4302532.2939868/1‑94 (MQ=60)
                                                                        CAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCC                      <  SRR4302532.800690/94‑1 (MQ=60)
                                                                        CAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCC                      <  SRR4302532.5312282/94‑1 (MQ=60)
                                                                        CAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCC                      <  SRR4302532.2841095/94‑1 (MQ=60)
                                                                           AATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGA                   >  SRR4302532.2070980/1‑94 (MQ=60)
                                                                           AATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGA                   >  SRR4302532.5801414/1‑94 (MQ=60)
                                                                           AATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGA                   >  SRR4302532.284922/1‑94 (MQ=60)
                                                                            ATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGAT                  >  SRR4302532.2997699/1‑94 (MQ=60)
                                                                            ATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGAT                  <  SRR4302532.4260282/94‑1 (MQ=60)
                                                                            ATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTCCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGAT                  <  SRR4302532.5506290/94‑1 (MQ=60)
                                                                               ATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACC               >  SRR4302532.3923566/1‑94 (MQ=60)
                                                                               ATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACC               >  SRR4302532.3354103/1‑94 (MQ=60)
                                                                               ATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACC               >  SRR4302532.1938100/1‑94 (MQ=60)
                                                                               ATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACC               >  SRR4302532.4374036/1‑94 (MQ=60)
                                                                                 CGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCAT             >  SRR4302532.5701646/1‑94 (MQ=60)
                                                                                   TGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCA           <  SRR4302532.108679/94‑1 (MQ=60)
                                                                                   TGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCA           >  SRR4302532.559447/1‑94 (MQ=60)
                                                                                    GAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCAT          >  SRR4302532.2027719/1‑94 (MQ=60)
                                                                                    GAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCAT          >  SRR4302532.1824804/1‑94 (MQ=60)
                                                                                     AAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATG         <  SRR4302532.1075907/94‑1 (MQ=60)
                                                                                     AAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATG         <  SRR4302532.1239182/94‑1 (MQ=60)
                                                                                      AATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGG        <  SRR4302532.1923253/94‑1 (MQ=60)
                                                                                      AATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGG        >  SRR4302532.642737/1‑94 (MQ=60)
                                                                                       ATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGA       >  SRR4302532.1908645/1‑94 (MQ=60)
                                                                                        TGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGAC      <  SRR4302532.5107670/94‑1 (MQ=60)
                                                                                         GAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACG     >  SRR4302532.954293/1‑94 (MQ=60)
                                                                                           atATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGC    >  SRR4302532.1487876/3‑94 (MQ=60)
                                                                                           atATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGC    <  SRR4302532.2779486/92‑1 (MQ=60)
                                                                                           atATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCAAGACACTACACCCGGATACCATCATGGACGC    >  SRR4302532.1487875/3‑94 (MQ=60)
                                                                                            TATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCT   <  SRR4302532.4490999/94‑1 (MQ=60)
                                                                                            TATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCT   >  SRR4302532.1955120/1‑94 (MQ=60)
                                                                                             ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTC  >  SRR4302532.4725148/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTC  >  NC_000913/4039867‑4040053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑