Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,068 +C intergenic (‑178/‑242) b4256 ← / → yjgN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,0681.C99.5% 687.3 / ‑3.8 188intergenic (‑178/‑242)b4256/yjgNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (30/157);  total (30/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 207 total aligned reads displayed.

GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476976‑4477153
                                                                                              |                                                                                     
  gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCATGcgac‑gcat                                                                                     >  2:4225764/1‑90 (MQ=255)
  gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gcat                                                                                     >  2:3810202/1‑90 (MQ=255)
  gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gcat                                                                                     <  2:5744246/93‑4 (MQ=255)
   tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gcatt                                                                                    <  1:1023975/93‑5 (MQ=255)
   tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gcatt                                                                                    >  2:4240224/1‑89 (MQ=255)
      ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                 <  2:2066009/93‑1 (MQ=255)
      ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                 <  2:4432730/93‑1 (MQ=255)
      ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                 <  2:4846359/93‑1 (MQ=255)
       cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg                                                                                <  1:751096/93‑1 (MQ=255)
         ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGt                                                                              <  1:1515051/93‑1 (MQ=255)
          tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACACGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTg                                                                             <  2:704784/93‑1 (MQ=255)
           aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGa                                                                            <  2:1293318/93‑1 (MQ=255)
            tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAt                                                                           <  1:2688678/93‑1 (MQ=255)
             cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAtt                                                                          <  2:5831133/93‑1 (MQ=255)
                  gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcggc                                                                     <  1:347992/93‑1 (MQ=255)
                    aaGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATGACAGGTGATTGCGgcgc                                                                   >  2:5496684/1‑93 (MQ=255)
                      gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa                                                                 >  2:1356006/1‑93 (MQ=255)
                       aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt                                                                <  2:1407206/93‑1 (MQ=255)
                       aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt                                                                <  2:1562670/93‑1 (MQ=255)
                       aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt                                                                >  1:4675604/1‑93 (MQ=255)
                        ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg                                                               >  1:2138838/1‑93 (MQ=255)
                        ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg                                                               >  1:5804288/1‑93 (MQ=255)
                        ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg                                                               <  2:3325921/93‑1 (MQ=255)
                         cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGt                                                              >  2:2010157/1‑93 (MQ=255)
                          gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGtt                                                             <  2:3404605/93‑1 (MQ=255)
                          ctATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGtt                                                             <  2:4126717/92‑1 (MQ=255)
                           tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  2:4364537/93‑1 (MQ=255)
                           tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  2:3202802/93‑1 (MQ=255)
                            aTTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAt                                                           <  2:117645/93‑1 (MQ=255)
                             ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCGGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt                                                          <  2:4690631/93‑1 (MQ=255)
                             ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGCGATTGCGGCGCAATGTTAtt                                                          >  2:4320729/1‑93 (MQ=255)
                             tcgggcggTTA‑CTGAGGAATGGCCGGGGCGAAAGC‑AGGAATATTCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc                                                         <  1:1940756/90‑1 (MQ=255)
                              tCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc                                                         <  2:2615909/93‑1 (MQ=255)
                              tCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc                                                         <  2:5248705/93‑1 (MQ=255)
                               cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                        >  2:4160923/1‑93 (MQ=255)
                               cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                        <  1:3729666/93‑1 (MQ=255)
                               cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                        <  2:6131499/93‑1 (MQ=255)
                               cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                        <  1:5970376/93‑1 (MQ=255)
                                ggcggATACCTGACGAGTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGCCGCAATGTTATTCAt                                                       <  1:888173/93‑1 (MQ=255)
                                ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAt                                                       <  2:3641742/93‑1 (MQ=255)
                                 gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  1:5858835/93‑1 (MQ=255)
                                   ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    <  1:1151426/93‑1 (MQ=255)
                                   ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    <  1:3110409/93‑1 (MQ=255)
                                   ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    >  1:2926901/1‑93 (MQ=255)
                                    gATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt                                                   <  2:632219/93‑1 (MQ=255)
                                    gATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGCCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt                                                   <  2:1071149/93‑1 (MQ=255)
                                     aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTTATTGCGGCGCAATGTTATTCATAGttt                                                  <  1:2218652/93‑1 (MQ=255)
                                     aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt                                                  <  1:5950478/93‑1 (MQ=255)
                                     aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt                                                  <  1:403221/93‑1 (MQ=255)
                                     aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt                                                  <  2:5644526/93‑1 (MQ=255)
                                      tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                 >  2:1853583/1‑93 (MQ=255)
                                      tACCTGACGAAGGACGCGGGCGATAGC‑ATGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                 <  2:1707721/93‑1 (MQ=255)
                                       aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa                                                <  1:5595089/93‑1 (MQ=255)
                                            aCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaa                                           <  1:4461823/93‑1 (MQ=255)
                                             cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  1:2837071/93‑1 (MQ=255)
                                              gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata                                         <  1:2439663/93‑1 (MQ=255)
                                               aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa                                        >  2:2501019/1‑93 (MQ=255)
                                               aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa                                        <  1:1921312/93‑1 (MQ=255)
                                                 tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                      >  2:1336139/1‑93 (MQ=255)
                                                 tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGCTTAACCCaataaaa                                      >  2:2698536/1‑93 (MQ=255)
                                                 agACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                      <  2:1916095/92‑1 (MQ=255)
                                                  gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaa                                     <  2:5929031/93‑1 (MQ=255)
                                                  gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaa                                     <  2:3691452/93‑1 (MQ=255)
                                                    cgcgGGCGATAGC‑AGGATGATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata                                   <  2:281692/93‑1 (MQ=255)
                                                      cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa                                 <  2:3825205/93‑1 (MQ=255)
                                                       gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                <  2:2646220/93‑1 (MQ=255)
                                                        ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt                               <  2:3267947/93‑1 (MQ=255)
                                                         gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  2:5350005/93‑1 (MQ=255)
                                                           gATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                            <  2:845638/93‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  2:1724160/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  1:639372/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  1:2716913/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  2:5419841/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  1:5294890/89‑1 (MQ=255)
                                                                gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                        <  1:575632/91‑1 (MQ=255)
                                                                gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                        <  2:2327506/91‑1 (MQ=255)
                                                                gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                        >  2:2426114/3‑93 (MQ=255)
                                                                  aGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  1:1766222/93‑1 (MQ=255)
                                                                  aGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  2:2255182/93‑1 (MQ=255)
                                                                   agATTATCCTGCAGCGTCAGGCGACAC‑ATTACAGGTGATTGCGGCGCAAAGTAATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     <  1:5465086/92‑1 (MQ=255)
                                                                     aTTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAttt                   <  1:6141098/93‑1 (MQ=255)
                                                                      ttATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  1:5715440/93‑1 (MQ=255)
                                                                      ttATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  1:133227/93‑1 (MQ=255)
                                                                      ttATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  1:407111/93‑1 (MQ=255)
                                                                      ttATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  1:4318612/93‑1 (MQ=255)
                                                                       tATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                 <  1:5340436/93‑1 (MQ=255)
                                                                       tATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                 <  1:251756/93‑1 (MQ=255)
                                                                        aTCCTGCAGCGTCAGGCGAC‑GCATTTCAGGAGATTGCGGCGGACTGTTATGCATAGTTTAACCCAATAAAAATAAAGTAATTAGACCtttttt                <  1:4324528/93‑1 (MQ=255)
                                                                        aTCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTACCCCAATAAAAATAAAGTAATTATACATTTGtt                <  1:5155837/93‑1 (MQ=255)
                                                                        aTCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGtt                <  2:454959/93‑1 (MQ=255)
                                                                         tCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGttt               <  1:2050485/93‑1 (MQ=255)
                                                                         tCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGttt               <  2:1965344/93‑1 (MQ=255)
                                                                            tGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAt            >  2:4671240/1‑93 (MQ=255)
                                                                            tGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAt            <  1:5911039/93‑1 (MQ=255)
                                                                             gCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATa           <  2:2752499/93‑1 (MQ=255)
                                                                             gCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATa           <  2:4504035/93‑1 (MQ=255)
                                                                                gCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCa        <  2:4902451/93‑1 (MQ=255)
                                                                                gCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCa        >  1:493928/1‑93 (MQ=255)
                                                                                 cGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc       <  2:5128758/93‑1 (MQ=255)
                                                                                     aGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctc   <  1:3257064/93‑1 (MQ=255)
                                                                                              |                                                                                     
GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476976‑4477153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA  >  NC_000913/4476974‑4477158
                                                                                               |                                                                                           
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATCCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G                                                                                             <  SRR4302532.4749303/94‑1 (MQ=60)
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G                                                                                             <  SRR4302532.5892607/94‑1 (MQ=60)
 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G‑C                                                                                           >  SRR4302532.921060/1‑94 (MQ=60)
   TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑Gcat                                                                                          <  SRR4302532.2606594/94‑4 (MQ=60)
    GTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATT                                                                                         <  SRR4302532.6165304/94‑1 (MQ=60)
     TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTA                                                                                        <  SRR4302532.1058641/94‑1 (MQ=60)
      AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTAC                                                                                       <  SRR4302532.650499/94‑1 (MQ=60)
         CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGG                                                                                    <  SRR4302532.776820/94‑1 (MQ=60)
           TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTG                                                                                  <  SRR4302532.1566220/94‑1 (MQ=60)
             ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAT                                                                                <  SRR4302532.2940975/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                               <  SRR4302532.2778991/94‑1 (MQ=60)
               CATCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG                                                                              <  SRR4302532.2449516/94‑1 (MQ=60)
                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCG                                                                            <  SRR4302532.773050/94‑1 (MQ=60)
                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCG                                                                            >  SRR4302532.5299659/1‑94 (MQ=60)
                    GTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCG                                                                         <  SRR4302532.360050/94‑1 (MQ=60)
                        GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAT                                                                     >  SRR4302532.4835026/1‑94 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                    <  SRR4302532.4560680/94‑1 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                    >  SRR4302532.6005990/1‑94 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                    >  SRR4302532.2210266/1‑94 (MQ=60)
                           CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTT                                                                  <  SRR4302532.5906879/94‑1 (MQ=60)
                            GTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTA                                                                 <  SRR4302532.3334714/94‑1 (MQ=60)
                            GTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTA                                                                 <  SRR4302532.5940856/94‑1 (MQ=60)
                              ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATT                                                               <  SRR4302532.1190453/94‑1 (MQ=60)
                                TCGGCGGATTCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                             <  SRR4302532.5900590/94‑1 (MQ=60)
                                 tcgggcggttactgaggaatggccggggcgaaagcaggaatattCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                             <  SRR4302532.2005291/50‑1 (MQ=60)
                                 CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                            <  SRR4302532.3854967/94‑1 (MQ=60)
                                 CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                            <  SRR4302532.6176795/94‑1 (MQ=60)
                                  GGCGGATACCTGACGAGTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGCCGCAATGTTATTCATA                                                           <  SRR4302532.918521/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                          <  SRR4302532.6061979/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                          <  SRR4302532.2806894/94‑1 (MQ=60)
                                    CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                         >  SRR4302532.3024281/1‑94 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                        <  SRR4302532.1189850/94‑1 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                        <  SRR4302532.3213578/94‑1 (MQ=60)
                                       ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTTATTGCGGCGCAATGTTATTCATAGTTTA                                                      <  SRR4302532.417309/94‑1 (MQ=60)
                                       ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTTATTGCGGCGCAATGTTATTCATAGTTTA                                                      <  SRR4302532.2292306/94‑1 (MQ=60)
                                       ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                      <  SRR4302532.417308/94‑1 (MQ=60)
                                       ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                      <  SRR4302532.6156369/94‑1 (MQ=60)
                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGTCGCAATGTTATTCATAGTTTAAC                                                    <  SRR4302532.4305649/94‑1 (MQ=60)
                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                    <  SRR4302532.5789312/94‑1 (MQ=60)
                                          CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                   <  SRR4302532.2133123/94‑1 (MQ=60)
                                              ACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAAT                                               <  SRR4302532.3092412/94‑1 (MQ=60)
                                              ACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAAT                                               <  SRR4302532.4613920/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              <  SRR4302532.2859823/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              <  SRR4302532.3733381/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              <  SRR4302532.2931914/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              <  SRR4302532.1753562/94‑1 (MQ=60)
                                                GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCACTGTTATTCATAGTTTAACCCAATAA                                             <  SRR4302532.2019324/94‑1 (MQ=60)
                                                GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                             <  SRR4302532.2520785/94‑1 (MQ=60)
                                                 AATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAA                                            <  SRR4302532.6076383/94‑1 (MQ=60)
                                                 AATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAA                                            <  SRR4302532.1985212/94‑1 (MQ=60)
                                                  ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                           <  SRR4302532.1462565/94‑1 (MQ=60)
                                                   agggcccggggcattgcAGGATTATCCGTCAGCGGCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                           <  SRR4302532.1462560/77‑1 (MQ=60)
                                                   TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                          <  SRR4302532.6023855/94‑1 (MQ=60)
                                                     ACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATA                                        <  SRR4302532.4846600/94‑1 (MQ=60)
                                                     ACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATA                                        <  SRR4302532.1112388/94‑1 (MQ=60)
                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                     <  SRR4302532.2548101/94‑1 (MQ=60)
                                                          GGCGATAGC‑AGTATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                   <  SRR4302532.2234414/94‑1 (MQ=60)
                                                           GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                  <  SRR4302532.6267792/94‑1 (MQ=60)
                                                             ggatagcAGGATTATCCTGAAGCGTCAGCCGAC‑GCATTACAGGTGTTTGAGGCGCAATGTTATTCATAGTTTAACCGAATAAAAATAGAGTAAT                                 <  SRR4302532.5754582/87‑1 (MQ=57)
                                                              catggcAGGATTATCCTGCAGCGTCCGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAAAGTTTAACCCAATAAAAATAAAGTAATT                                <  SRR4302532.6015909/88‑1 (MQ=60)
                                                              ATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAGCCCAATAAAAATAAAGTAATTA                               <  SRR4302532.6129132/94‑1 (MQ=60)
                                                              ATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                               <  SRR4302532.1982904/94‑1 (MQ=60)
                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                              <  SRR4302532.5959304/94‑1 (MQ=60)
                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                              <  SRR4302532.661630/94‑1 (MQ=60)
                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                              <  SRR4302532.5477733/94‑1 (MQ=60)
                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                              <  SRR4302532.2808288/94‑1 (MQ=60)
                                                                AGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                             >  SRR4302532.194649/1‑94 (MQ=60)
                                                                AGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                             <  SRR4302532.655431/94‑1 (MQ=60)
                                                                  gcAGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                            <  SRR4302532.595712/92‑1 (MQ=60)
                                                                    AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                          <  SRR4302532.1825411/94‑1 (MQ=60)
                                                                     AGATTATCCTGCAGCGTCAGGCGACAC‑ATTACAGGTGATTGCGGCGCAAAGTAATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                         <  SRR4302532.5654563/94‑1 (MQ=60)
                                                                       ATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                       <  SRR4302532.6352937/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      <  SRR4302532.2003521/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      <  SRR4302532.137711/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      <  SRR4302532.5914575/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      <  SRR4302532.4464586/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      <  SRR4302532.421316/94‑1 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                     <  SRR4302532.5525682/94‑1 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                     <  SRR4302532.5525683/94‑1 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                     <  SRR4302532.260510/94‑1 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                     <  SRR4302532.391756/94‑1 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTTCAGGAGATTGCGGCGGACTGTTATGCATAGTTTAACCCAATAAAAATAAAGTAATTAGACCTTTTTTT                    <  SRR4302532.4470658/94‑1 (MQ=55)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTACCCCAATAAAAATAAAGTAATTATACATTTGTTT                    <  SRR4302532.5333244/94‑1 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                    <  SRR4302532.4470657/94‑1 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                    <  SRR4302532.5495571/94‑1 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                    <  SRR4302532.5776039/94‑1 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                    <  SRR4302532.561959/94‑1 (MQ=60)
                                                                           TCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTA                   <  SRR4302532.2118766/94‑1 (MQ=60)
                                                                            CCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAA                  <  SRR4302532.3400388/94‑1 (MQ=60)
                                                                             CTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAT                 <  SRR4302532.2998924/94‑1 (MQ=60)
                                                                              TGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATA                <  SRR4302532.4661160/94‑1 (MQ=60)
                                                                              TGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATA                <  SRR4302532.6115750/94‑1 (MQ=60)
                                                                               GCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC               >  SRR4302532.914090/1‑94 (MQ=60)
                                                                                 AGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCA             >  SRR4302532.511190/1‑94 (MQ=60)
                                                                                  GCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAC            <  SRR4302532.6153755/94‑1 (MQ=60)
                                                                                     TCAGGCTAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT         <  SRR4302532.5464789/94‑1 (MQ=60)
                                                                                       AGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT       <  SRR4302532.3365281/94‑1 (MQ=60)
                                                                                          CGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCT    <  SRR4302532.2691018/94‑1 (MQ=60)
                                                                                            AC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA  >  SRR4302532.5901780/1‑94 (MQ=60)
                                                                                               |                                                                                           
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA  >  NC_000913/4476974‑4477158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑