Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,558,365 A→G intergenic (‑104/‑134) yjiH ← / → b4331 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,558,3650AG95.5% 815.4 / 18.3 245intergenic (‑104/‑134)yjiH/b4331hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base G (129/105);  total (135/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 249 total aligned reads displayed.

TTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAA  >  NC_000913/4558275‑4558454
                                                                                          |                                                                                         
 tgtgtgTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGc                                                                                        >  1:3550628/1‑93 (MQ=255)
 tgtgtgTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGCGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGc                                                                                        >  1:772073/1‑93 (MQ=255)
       ttGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGttttt                                                                                  >  2:4200405/1‑93 (MQ=255)
       ttGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGttttt                                                                                  <  1:221805/93‑1 (MQ=255)
        tGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGtttttt                                                                                 <  2:5838518/93‑1 (MQ=255)
          cTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGc                                                                               >  2:933352/1‑93 (MQ=255)
          cTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGc                                                                               >  1:4004913/1‑93 (MQ=255)
          cTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTCTGc                                                                               <  1:2089325/93‑1 (MQ=255)
           ttGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCa                                                                              <  1:2392638/93‑1 (MQ=255)
              aGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAg                                                                           <  2:55852/93‑1 (MQ=255)
                ccAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCg                                                                         <  1:4933558/93‑1 (MQ=255)
                 cAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCgg                                                                        >  2:5377791/1‑93 (MQ=255)
                  aGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGa                                                                       >  2:3308412/1‑93 (MQ=255)
                  aGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGa                                                                       >  2:1345460/1‑93 (MQ=255)
                  aGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGa                                                                       >  1:4758056/1‑93 (MQ=255)
                  aGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGa                                                                       >  1:6127531/1‑93 (MQ=255)
                  aGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGa                                                                       >  2:5511893/1‑93 (MQ=255)
                   ggAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGaa                                                                      >  2:4829672/1‑93 (MQ=255)
                         ttCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTa                                                                <  1:2968857/93‑1 (MQ=255)
                         ttCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTa                                                                >  2:4497743/1‑93 (MQ=255)
                          tCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAc                                                               >  1:2821750/1‑93 (MQ=255)
                          tCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAc                                                               <  2:1902461/93‑1 (MQ=255)
                          tCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAc                                                               <  2:5025912/93‑1 (MQ=255)
                          tCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAc                                                               >  1:1155112/1‑93 (MQ=255)
                            cTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGc                                                             >  2:3213422/1‑93 (MQ=255)
                            cTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGc                                                             >  2:5044874/1‑93 (MQ=255)
                            cTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGc                                                             <  1:3724327/93‑1 (MQ=255)
                            cTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGc                                                             >  2:5690605/1‑93 (MQ=255)
                             tGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCt                                                            >  1:1899528/1‑93 (MQ=255)
                             tGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCt                                                            >  2:4248492/1‑93 (MQ=255)
                              ggAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCtg                                                           >  1:152721/1‑93 (MQ=255)
                               gAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCtgt                                                          <  1:3775805/93‑1 (MQ=255)
                               gAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCtgt                                                          >  2:2851318/1‑93 (MQ=255)
                                aaCTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCtgtg                                                         >  1:18/1‑93 (MQ=255)
                                     aaaaaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGaa                                                    >  2:3888143/1‑93 (MQ=255)
                                      aaaaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGaaa                                                   <  1:1030122/93‑1 (MQ=255)
                                      aaaaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGaaa                                                   >  2:479430/1‑93 (MQ=255)
                                       aaaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGaaaa                                                  <  1:5233755/93‑1 (MQ=255)
                                       aaaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGaaaa                                                  <  2:4040047/93‑1 (MQ=255)
                                        aaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAg                                                 >  2:2581286/1‑93 (MQ=255)
                                        aaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAg                                                 <  2:1875118/93‑1 (MQ=255)
                                        aaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAg                                                 >  2:3655949/1‑93 (MQ=255)
                                         aCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAgg                                                >  2:4926033/1‑93 (MQ=255)
                                         aCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAgg                                                >  2:3100589/1‑93 (MQ=255)
                                         aCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAgg                                                >  2:2991064/1‑93 (MQ=255)
                                          ccTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGc                                               <  1:740252/93‑1 (MQ=255)
                                          ccTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGc                                               >  1:3921725/1‑93 (MQ=255)
                                          ccTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGc                                               >  1:3865612/1‑93 (MQ=255)
                                           cTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGct                                              >  1:3957815/1‑93 (MQ=255)
                                            ttATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGctc                                             >  1:5750665/1‑93 (MQ=255)
                                             tATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGctct                                            >  1:3644275/1‑93 (MQ=255)
                                             tATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGctct                                            >  2:5124605/1‑93 (MQ=255)
                                             tATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGctct                                            >  1:5116115/1‑93 (MQ=255)
                                              aTCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTg                                           >  1:5896843/1‑93 (MQ=255)
                                              aTCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTg                                           >  2:52832/1‑93 (MQ=255)
                                                 aGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTgcgc                                        >  1:1083803/1‑93 (MQ=255)
                                                  gCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCa                                       >  2:1772002/1‑93 (MQ=255)
                                                  gCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCa                                       <  2:2354055/93‑1 (MQ=255)
                                                    cATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAgg                                     >  2:5357336/1‑93 (MQ=255)
                                                      tgtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAt                                   <  2:2184845/93‑1 (MQ=255)
                                                      tgtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAt                                   >  2:2243494/1‑93 (MQ=255)
                                                      tgtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAt                                   >  2:4640059/1‑93 (MQ=255)
                                                       gtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAtt                                  <  2:2510274/93‑1 (MQ=255)
                                                       gtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAtt                                  >  1:5033981/1‑93 (MQ=255)
                                                        tgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTg                                 >  1:1092877/1‑93 (MQ=255)
                                                         gggTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGa                                <  1:373544/93‑1 (MQ=255)
                                                          ggTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAg                               <  2:3960280/93‑1 (MQ=255)
                                                            tATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGtt                             >  1:586745/1‑93 (MQ=255)
                                                            tATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGtt                             >  2:2951757/1‑93 (MQ=255)
                                                              tGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGc                           <  2:1195143/93‑1 (MQ=255)
                                                              tGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGc                           >  2:2131543/1‑93 (MQ=255)
                                                              tGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGc                           >  1:6089593/1‑93 (MQ=255)
                                                              tGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGc                           >  2:6033834/1‑93 (MQ=255)
                                                                gggAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAg                         >  2:5989435/1‑93 (MQ=255)
                                                                 ggAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGa                        >  2:767831/1‑93 (MQ=255)
                                                                   aaaaTGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGaaa                      <  1:4235488/93‑1 (MQ=255)
                                                                   aaaaTGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGaaa                      <  2:4717736/93‑1 (MQ=255)
                                                                    aaaTGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGaaaa                     <  2:5948003/93‑1 (MQ=255)
                                                                      aTGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAAtt                   >  1:2581237/1‑93 (MQ=255)
                                                                      aTGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAAtt                   <  2:3495203/93‑1 (MQ=255)
                                                                        gCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCa                 >  2:633461/1‑93 (MQ=255)
                                                                        gCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCa                 <  2:1063638/93‑1 (MQ=255)
                                                                         cAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCAt                <  2:2914339/93‑1 (MQ=255)
                                                                          aaGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATa               >  1:5663129/1‑93 (MQ=255)
                                                                           aGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATaa              >  2:4379911/1‑93 (MQ=255)
                                                                           aGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATaa              <  1:331718/93‑1 (MQ=255)
                                                                            gTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATaaa             <  2:4563412/93‑1 (MQ=255)
                                                                            gTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATaaa             <  1:688661/93‑1 (MQ=255)
                                                                            gTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATaaa             <  1:1336181/93‑1 (MQ=255)
                                                                             tATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAAc            <  2:3447320/93‑1 (MQ=255)
                                                                               tGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCa          >  2:272719/1‑93 (MQ=255)
                                                                               tGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCa          >  1:1973923/1‑93 (MQ=255)
                                                                               tGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCa          >  1:2628641/1‑93 (MQ=255)
                                                                               tGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCa          <  2:1605721/93‑1 (MQ=255)
                                                                                  ttCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGt       <  1:3270863/93‑1 (MQ=255)
                                                                                  ttCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGt       <  2:1684058/93‑1 (MQ=255)
                                                                                   tCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTg      >  1:1563775/1‑93 (MQ=255)
                                                                                   tCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTg      <  1:4831663/93‑1 (MQ=255)
                                                                                   tCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTg      >  1:5394661/1‑93 (MQ=255)
                                                                                     tGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGAc    <  1:5538385/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAA  >  NC_000913/4558275‑4558454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGAC  >  NC_000913/4558274‑4558458
                                                                                           |                                                                                             
TTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTG                                                                                             <  SRR4302532.1468725/94‑1 (MQ=60)
 TTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGC                                                                                            >  SRR4302532.3669417/1‑94 (MQ=60)
 TTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGCGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGC                                                                                            >  SRR4302532.798711/1‑94 (MQ=60)
      TGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTT                                                                                       >  SRR4302532.6251410/1‑94 (MQ=60)
        TTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTT                                                                                     <  SRR4302532.229381/94‑1 (MQ=60)
        TTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTT                                                                                     <  SRR4302532.5730429/94‑1 (MQ=60)
         TGCTTGAGCCAGGAAAATTGCTGGAACTGAAGACCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGACTATGCGTTTTTTG                                                                                    <  SRR4302532.4884938/94‑1 (MQ=60)
          GCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGC                                                                                   >  SRR4302532.1548734/1‑94 (MQ=60)
          GCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGC                                                                                   >  SRR4302532.4139856/1‑94 (MQ=60)
           CTTGAGCCAGGAAAATTCCTGGAACTGAAAAAGCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCA                                                                                  >  SRR4302532.379510/1‑94 (MQ=60)
           CTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCA                                                                                  >  SRR4302532.379509/1‑94 (MQ=60)
           CTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTCTGCA                                                                                  <  SRR4302532.2159011/94‑1 (MQ=60)
            TTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCAT                                                                                 <  SRR4302532.2472337/94‑1 (MQ=60)
            TTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCAT                                                                                 >  SRR4302532.5020873/1‑94 (MQ=60)
                 CCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGG                                                                            <  SRR4302532.5102960/94‑1 (MQ=60)
                  CAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGA                                                                           >  SRR4302532.4100558/1‑94 (MQ=60)
                  CAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGA                                                                           >  SRR4302532.4920862/1‑94 (MQ=60)
                  CAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGA                                                                           >  SRR4302532.6338972/1‑94 (MQ=60)
                        AATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATT                                                                     <  SRR4302532.2064230/94‑1 (MQ=60)
                          TTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAC                                                                   >  SRR4302532.2916172/1‑94 (MQ=60)
                          TTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAC                                                                   <  SRR4302532.3067152/94‑1 (MQ=60)
                          TTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTAC                                                                   >  SRR4302532.1193649/1‑94 (MQ=60)
                           TCCTGGAATTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACG                                                                  <  SRR4302532.2187175/94‑1 (MQ=60)
                           TCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACG                                                                  >  SRR4302532.4524245/1‑94 (MQ=60)
                            CCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGC                                                                 <  SRR4302532.1158958/94‑1 (MQ=60)
                             CTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGGAAAAGCAAGAATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAAATAAGCT                                                                >  SRR4302532.6203795/1‑94 (MQ=60)
                             CTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCT                                                                <  SRR4302532.3849393/94‑1 (MQ=60)
                             CTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCT                                                                <  SRR4302532.5581570/94‑1 (MQ=60)
                             CTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCT                                                                >  SRR4302532.1962514/1‑94 (MQ=60)
                              TGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTG                                                               >  SRR4302532.3554883/1‑94 (MQ=60)
                              TGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTG                                                               >  SRR4302532.157823/1‑94 (MQ=60)
                               TGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGT                                                              <  SRR4302532.1351585/94‑1 (MQ=60)
                               GGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGT                                                              <  SRR4302532.2012119/94‑1 (MQ=60)
                               GGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGT                                                              <  SRR4302532.2627970/94‑1 (MQ=60)
                                GAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTG                                                             <  SRR4302532.3902961/94‑1 (MQ=60)
                                GAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTG                                                             >  SRR4302532.19/1‑94 (MQ=60)
                                  ACTGAAAAACCTTATCAGCCATGTTGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTG                                                           >  SRR4302532.4008038/1‑94 (MQ=60)
                                  ACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTG                                                           >  SRR4302532.882953/1‑94 (MQ=60)
                                   CTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGG                                                          <  SRR4302532.4095288/94‑1 (MQ=60)
                                    TGAAAAACCTTATCAGCTATGTGGGTATGGGAAAAATGCAAGTATGTTTCTGGCTATGCGTTGTTGGCATAGCGGAAAAATTACGCTGTGTGGA                                                         <  SRR4302532.69075/94‑1 (MQ=60)
                                    TGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGA                                                         >  SRR4302532.3359284/1‑94 (MQ=60)
                                    TGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGA                                                         <  SRR4302532.69077/94‑1 (MQ=60)
                                     GAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAA                                                        <  SRR4302532.1896297/94‑1 (MQ=60)
                                     GAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAA                                                        >  SRR4302532.1726272/1‑94 (MQ=60)
                                     GAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAA                                                        <  SRR4302532.5274451/94‑1 (MQ=60)
                                       AAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAA                                                      <  SRR4302532.1064927/94‑1 (MQ=60)
                                        AAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAG                                                     <  SRR4302532.5414235/94‑1 (MQ=60)
                                        AAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAG                                                     >  SRR4302532.5125646/1‑94 (MQ=60)
                                         AACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGG                                                    >  SRR4302532.6052629/1‑94 (MQ=60)
                                          ACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGC                                                   >  SRR4302532.4053844/1‑94 (MQ=60)
                                          ACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGC                                                   >  SRR4302532.3996072/1‑94 (MQ=60)
                                           CCTTATCAGCGAGGTGGGTATGGGGAAAATGCAAGTTTGTTTCTGGTTGTGCTTTTTTTGCATGGCGGAAAAATTACGCTGTGTGGAAAAGGCT                                                  <  SRR4302532.4414745/94‑1 (MQ=60)
                                           CCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCT                                                  <  SRR4302532.765496/94‑1 (MQ=60)
                                           CCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCT                                                  >  SRR4302532.4091170/1‑94 (MQ=60)
                                           CCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCT                                                  <  SRR4302532.4000712/94‑1 (MQ=60)
                                            CTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTC                                                 <  SRR4302532.4984954/94‑1 (MQ=60)
                                            CTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTC                                                 <  SRR4302532.5517638/94‑1 (MQ=60)
                                            CTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTC                                                 >  SRR4302532.5950456/1‑94 (MQ=60)
                                            CTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTC                                                 >  SRR4302532.5950828/1‑94 (MQ=60)
                                             TTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCT                                                >  SRR4302532.3766555/1‑94 (MQ=60)
                                             TTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCT                                                >  SRR4302532.5292061/1‑94 (MQ=60)
                                              TATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTG                                               >  SRR4302532.6101055/1‑94 (MQ=60)
                                                 CAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGC                                            >  SRR4302532.1120110/1‑94 (MQ=60)
                                                  AGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAACAATTACGCTGTGTGGAAAAGCCTCTGCGAA                                           >  SRR4302532.1499519/1‑94 (MQ=60)
                                                  AGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCA                                           >  SRR4302532.786319/1‑94 (MQ=60)
                                                   GCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAG                                          <  SRR4302532.1079942/94‑1 (MQ=60)
                                                   GCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAG                                          <  SRR4302532.5737540/94‑1 (MQ=60)
                                                   GCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAG                                          <  SRR4302532.3309282/94‑1 (MQ=60)
                                                    CCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGG                                         >  SRR4302532.5270653/1‑94 (MQ=60)
                                                    CCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGG                                         >  SRR4302532.4077071/1‑94 (MQ=60)
                                                      ATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAT                                       >  SRR4302532.3955192/1‑94 (MQ=60)
                                                      ATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAT                                       <  SRR4302532.727382/94‑1 (MQ=60)
                                                      ATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGAT                                       >  SRR4302532.3400163/1‑94 (MQ=60)
                                                       TGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATT                                      >  SRR4302532.5206645/1‑94 (MQ=60)
                                                        GTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTG                                     >  SRR4302532.1129430/1‑94 (MQ=60)
                                                         TGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGA                                    >  SRR4302532.1122138/1‑94 (MQ=60)
                                                          GGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAG                                   <  SRR4302532.1744031/94‑1 (MQ=60)
                                                          GGGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAG                                   <  SRR4302532.386509/94‑1 (MQ=60)
                                                           GGTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGT                                  >  SRR4302532.63177/1‑94 (MQ=60)
                                                            GTATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTT                                 >  SRR4302532.607214/1‑94 (MQ=60)
                                                              ATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGC                               >  SRR4302532.6299830/1‑94 (MQ=60)
                                                              ATGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGC                               >  SRR4302532.3051314/1‑94 (MQ=60)
                                                               TGGGGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCA                              <  SRR4302532.2787859/94‑1 (MQ=60)
                                                                  GGAAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAA                           >  SRR4302532.3697326/1‑94 (MQ=60)
                                                                    AAAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAA                         <  SRR4302532.4377719/94‑1 (MQ=60)
                                                                     AAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAAT                        >  SRR4302532.1997317/1‑94 (MQ=60)
                                                                     AAATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAAT                        >  SRR4302532.3851296/1‑94 (MQ=60)
                                                                      AATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATT                       <  SRR4302532.609448/94‑1 (MQ=60)
                                                                      AATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATT                       >  SRR4302532.5360213/1‑94 (MQ=60)
                                                                      AATGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATT                       >  SRR4302532.2667496/1‑94 (MQ=60)
                                                                      AATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATT                       >  SRR4302532.2170108/1‑94 (MQ=60)
                                                                        TGCAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGGAAAATTACGCTGTGTGGAAAAGGCTCTGCGGAGGATTGAGTTGCAGACACTTCA                     >  SRR4302532.782005/1‑94 (MQ=60)
                                                                          CAAGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATA                   >  SRR4302532.5860491/1‑94 (MQ=60)
                                                                            AGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAA                 <  SRR4302532.343225/94‑1 (MQ=60)
                                                                            AGTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAA                 >  SRR4302532.3885186/1‑94 (MQ=60)
                                                                             GTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAAC                <  SRR4302532.1380874/94‑1 (MQ=60)
                                                                             GTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAAC                <  SRR4302532.115653/94‑1 (MQ=60)
                                                                             GTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAAC                <  SRR4302532.712289/94‑1 (MQ=60)
                                                                             GTATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAAC                <  SRR4302532.5998093/94‑1 (MQ=60)
                                                                              TATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACC               >  SRR4302532.4418347/1‑94 (MQ=60)
                                                                              TATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACC               >  SRR4302532.2676773/1‑94 (MQ=60)
                                                                               ATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCA              >  SRR4302532.2039494/1‑94 (MQ=60)
                                                                               ATGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCA              >  SRR4302532.2716685/1‑94 (MQ=60)
                                                                                TGTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAA             <  SRR4302532.431900/94‑1 (MQ=60)
                                                                                 GTTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAG            <  SRR4302532.2744928/94‑1 (MQ=60)
                                                                                  TTTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGT           <  SRR4302532.3627235/94‑1 (MQ=60)
                                                                                   TTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTG          >  SRR4302532.1616668/1‑94 (MQ=60)
                                                                                   TTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTG          >  SRR4302532.1616669/1‑94 (MQ=60)
                                                                                   TTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTG          <  SRR4302532.3379636/94‑1 (MQ=60)
                                                                                   TTCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTG          >  SRR4302532.5581574/1‑94 (MQ=60)
                                                                                    TCTGGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGA         <  SRR4302532.4997979/94‑1 (MQ=60)
                                                                                      TGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACA       >  SRR4302532.5820029/1‑94 (MQ=60)
                                                                                      TGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACA       <  SRR4302532.5730603/94‑1 (MQ=60)
                                                                                       GGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAA      >  SRR4302532.2390778/1‑94 (MQ=60)
                                                                                       GGCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAA      >  SRR4302532.3887487/1‑94 (MQ=60)
                                                                                        GCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAAT     <  SRR4302532.2601619/94‑1 (MQ=60)
                                                                                        GCTGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAAT     <  SRR4302532.5036183/94‑1 (MQ=60)
                                                                                          TGTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGA   >  SRR4302532.1277462/1‑94 (MQ=60)
                                                                                           GTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGAC  >  SRR4302532.4173544/1‑94 (MQ=60)
                                                                                           GTGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTCGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGAC  <  SRR4302532.1920636/94‑1 (MQ=60)
                                                                                           |                                                                                             
TTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGAC  >  NC_000913/4558274‑4558458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑