Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,593,254 2 bp→GC coding (59‑60/807 nt) yjjM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,2540CG100.0% 911.8 / NA 251A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (129/122);  total (129/122)
*NC_0009134,593,2550GC100.0% 907.2 / NA 251A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (129/122);  total (129/122)

Only 100 of 258 total aligned reads displayed.

ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGAAGT  >  NC_000913/4593167‑4593340
                                                                                       ||                                                                                     
aCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCa                                                                                   >  2:3189818/1‑93 (MQ=255)
 cTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                  >  1:66766/1‑93 (MQ=255)
 cTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                  >  1:5200641/1‑93 (MQ=255)
 cTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                  >  1:2976943/1‑93 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 <  1:4757764/93‑1 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 <  2:4427571/93‑1 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 >  1:2461789/1‑93 (MQ=255)
    aTTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcg                                                                               >  1:5416029/1‑93 (MQ=255)
    aTTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcg                                                                               >  2:1797196/1‑93 (MQ=255)
     ttGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcgc                                                                              <  2:1392794/93‑1 (MQ=255)
     ttGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTCTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcgc                                                                              >  1:5889374/1‑93 (MQ=255)
     ttGAAGCGGTAGTACAGGCGAAACCGTCATCATGGGCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcgg                                                                              >  1:1461537/1‑92 (MQ=255)
       gAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGt                                                                            >  2:4852778/1‑93 (MQ=255)
          gCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAg                                                                         <  2:3945250/93‑1 (MQ=255)
          gCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAg                                                                         <  1:1818233/93‑1 (MQ=255)
           cGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGt                                                                        <  1:5988224/93‑1 (MQ=255)
             gtagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTgg                                                                      <  1:563401/93‑1 (MQ=255)
             gtagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCCCATTCGCGTAAGTgg                                                                      >  2:1109126/1‑93 (MQ=255)
               agtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCt                                                                    <  1:1736374/93‑1 (MQ=255)
                 taCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTga                                                                  <  2:1491605/93‑1 (MQ=255)
                 taCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTga                                                                  >  2:2664948/1‑93 (MQ=255)
                  aCAGGCGAAACCGTCAACATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgag                                                                 <  2:5495245/93‑1 (MQ=255)
                     ggCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAat                                                              <  2:4798682/93‑1 (MQ=255)
                      gCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAata                                                             >  1:2350960/1‑93 (MQ=255)
                      gCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAata                                                             <  1:1605886/93‑1 (MQ=255)
                      gCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAata                                                             <  2:5074170/93‑1 (MQ=255)
                      gCGAAACCGTCATCATGGTAAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAata                                                             >  1:5696988/1‑93 (MQ=255)
                       cGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                                                            <  2:2539230/93‑1 (MQ=255)
                        gAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATg                                                           >  2:1198225/1‑93 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          <  2:6047497/93‑1 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          <  2:287979/93‑1 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          <  2:4152836/93‑1 (MQ=255)
                          aaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                         <  1:4655487/93‑1 (MQ=255)
                          aaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                         >  1:503227/1‑93 (MQ=255)
                          aaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                         <  2:2750042/93‑1 (MQ=255)
                            ccGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGc                                                       <  2:1673080/93‑1 (MQ=255)
                             cGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCa                                                      <  2:652263/93‑1 (MQ=255)
                              gTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAc                                                     >  1:4184280/1‑93 (MQ=255)
                              gTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAc                                                     <  1:2222846/93‑1 (MQ=255)
                              gTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAc                                                     >  1:3697631/1‑93 (MQ=255)
                              gTCATCATGGTCAGGTCTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAc                                                     <  2:1020574/93‑1 (MQ=255)
                               tcatcaTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACt                                                    >  1:3964438/1‑93 (MQ=255)
                               tcatcaTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGCAAGTGGCTGAGAATATGACGCACt                                                    >  1:435907/1‑93 (MQ=255)
                                  tcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                 <  2:637224/93‑1 (MQ=255)
                                   catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtgg                                                >  2:3868559/1‑93 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              >  2:4298641/1‑93 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              >  2:3979157/1‑93 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              <  1:5092163/93‑1 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              <  2:5672514/93‑1 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              >  1:386905/1‑93 (MQ=255)
                                      ggTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                             >  1:1195972/1‑93 (MQ=255)
                                      ggTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                             <  2:2449968/93‑1 (MQ=255)
                                        tCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCgg                                           >  1:2795352/1‑93 (MQ=255)
                                         cAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGc                                          >  1:3462388/1‑93 (MQ=255)
                                           ggTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTg                                        >  2:2672622/1‑93 (MQ=255)
                                            gTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGa                                       >  1:5523347/1‑93 (MQ=255)
                                            gTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGa                                       <  1:1899841/93‑1 (MQ=255)
                                             tttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                      >  1:2600092/1‑93 (MQ=255)
                                              ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtg                                     <  1:166659/93‑1 (MQ=255)
                                              ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGAGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtg                                     >  2:5638828/1‑93 (MQ=255)
                                              ttACGCACAATAACAAAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtg                                     <  2:4711820/93‑1 (MQ=255)
                                                   cgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTa                                <  1:3068116/93‑1 (MQ=255)
                                                   cgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTa                                >  1:2686383/1‑93 (MQ=255)
                                                    gcAATAACATAGTCGTTTCCAACCTGCGTGAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAc                               <  2:1989570/93‑1 (MQ=255)
                                                     cAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACa                              <  2:4938044/93‑1 (MQ=255)
                                                      aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAt                             <  2:5940983/93‑1 (MQ=255)
                                                       aTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAtt                            >  1:2070494/1‑93 (MQ=255)
                                                        tAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAttt                           >  1:3129578/1‑93 (MQ=255)
                                                         aaCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTc                          >  2:5161289/1‑93 (MQ=255)
                                                         aaCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTc                          >  2:4042672/1‑93 (MQ=255)
                                                         aaCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTc                          >  2:3629799/1‑93 (MQ=255)
                                                         aaCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTc                          <  1:4311773/93‑1 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  1:3591993/1‑93 (MQ=255)
                                                            aTAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGc                       >  1:3171264/1‑93 (MQ=255)
                                                            aTAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGc                       >  2:2506914/1‑93 (MQ=255)
                                                              aGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCa                     >  1:1337522/1‑93 (MQ=255)
                                                               gTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAg                    <  2:5230767/93‑1 (MQ=255)
                                                                tCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgc                   >  2:1569735/1‑93 (MQ=255)
                                                                  gTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgcgc                 >  1:2568384/1‑93 (MQ=255)
                                                                    ttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGGCAGCGCAc               >  1:2425325/1‑93 (MQ=255)
                                                                    ttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAc               <  2:3461991/93‑1 (MQ=255)
                                                                    ttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAc               <  2:1727764/93‑1 (MQ=255)
                                                                     tCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt              <  1:4506022/93‑1 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             <  2:4917901/93‑1 (MQ=255)
                                                                       cAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACttt            <  2:3136054/93‑1 (MQ=255)
                                                                        aaCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTg           >  1:630692/1‑93 (MQ=255)
                                                                           cTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGa        >  2:270970/1‑93 (MQ=255)
                                                                           cTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGa        >  1:2917699/1‑93 (MQ=255)
                                                                           cTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGa        <  2:5125417/93‑1 (MQ=255)
                                                                           cTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGa        >  2:3318761/1‑93 (MQ=255)
                                                                             gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATg      <  2:2199022/93‑1 (MQ=255)
                                                                             gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATg      <  2:5805215/93‑1 (MQ=255)
                                                                              cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgg     <  2:409825/93‑2 (MQ=255)
                                                                               gTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgga    >  2:3229132/1‑91 (MQ=255)
                                                                                tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaa   >  1:6109137/1‑90 (MQ=255)
                                                                                tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaa   >  2:4153523/1‑90 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  2:3297429/93‑5 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  1:2182626/93‑5 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  2:72597/93‑5 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTAGCTGAGAATATGACGCACTGTGGTGCGGCGGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  1:4251328/93‑5 (MQ=255)
                                                                                       ||                                                                                     
ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGAAGT  >  NC_000913/4593167‑4593340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑AAGTGG  >  NC_000913/4593161‑4593342
                                                                                             |                                                                                          
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                            >  SRR4302532.6241510/1‑94 (MQ=60)
      CCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                      <  SRR4302532.3189067/94‑1 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                      >  SRR4302532.69167/1‑94 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                      >  SRR4302532.3075435/1‑94 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                      <  SRR4302532.3946589/94‑1 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                      >  SRR4302532.5379965/1‑94 (MQ=60)
       CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                     >  SRR4302532.2950385/1‑94 (MQ=60)
       CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                     >  SRR4302532.2543688/1‑94 (MQ=60)
        TCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                    <  SRR4302532.4885657/94‑1 (MQ=60)
        TCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                    <  SRR4302532.4920552/94‑1 (MQ=60)
         CATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCG                                                                                   <  SRR4302532.4505251/94‑1 (MQ=60)
         CATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCG                                                                                   <  SRR4302532.2105439/94‑1 (MQ=60)
         CATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCG                                                                                   >  SRR4302532.5603713/1‑94 (MQ=60)
         CATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCG                                                                                   >  SRR4302532.4573296/1‑94 (MQ=60)
          ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGC                                                                                  >  SRR4302532.3025916/1‑94 (MQ=60)
          ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGC                                                                                  >  SRR4302532.6093328/1‑94 (MQ=60)
          ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTCTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGC                                                                                  >  SRR4302532.6093329/1‑94 (MQ=60)
          ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGGCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGG                                                                                  >  SRR4302532.1510790/1‑94 (MQ=60)
                GCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGT                                                                            <  SRR4302532.1878844/94‑1 (MQ=60)
                GCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGT                                                                            <  SRR4302532.4733236/94‑1 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                           <  SRR4302532.6195115/94‑1 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                           <  SRR4302532.4120206/94‑1 (MQ=60)
                  GGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGG                                                                          <  SRR4302532.2443310/94‑1 (MQ=60)
                  GGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGG                                                                          <  SRR4302532.3810443/94‑1 (MQ=60)
                   GTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                         <  SRR4302532.583049/94‑1 (MQ=60)
                    TAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCT                                                                        <  SRR4302532.6337983/94‑1 (MQ=60)
                     AGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTG                                                                       <  SRR4302532.1794815/94‑1 (MQ=60)
                     AGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTG                                                                       >  SRR4302532.2801653/1‑94 (MQ=60)
                     AGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTG                                                                       >  SRR4302532.5366423/1‑94 (MQ=60)
                      GTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGA                                                                      <  SRR4302532.3259026/94‑1 (MQ=60)
                       TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAG                                                                     >  SRR4302532.703638/1‑94 (MQ=60)
                        ACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGA                                                                    >  SRR4302532.2643841/1‑94 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                  >  SRR4302532.2225325/1‑94 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                  <  SRR4302532.2905884/94‑1 (MQ=60)
                           GGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                 <  SRR4302532.4402361/94‑1 (MQ=60)
                           GGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                 >  SRR4302532.190746/1‑94 (MQ=60)
                           GGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                 <  SRR4302532.5299117/94‑1 (MQ=60)
                           GGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                 >  SRR4302532.190744/1‑94 (MQ=60)
                           GGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                 >  SRR4302532.2429399/1‑94 (MQ=60)
                           GGCGAAACCGTCATCATGGTAAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATA                                                                 >  SRR4302532.5895718/1‑94 (MQ=60)
                            GCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATAT                                                                <  SRR4302532.1660250/94‑1 (MQ=60)
                             CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATG                                                               <  SRR4302532.3064493/94‑1 (MQ=60)
                             CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATG                                                               <  SRR4302532.5033146/94‑1 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                             >  SRR4302532.520820/1‑94 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                             <  SRR4302532.3897095/94‑1 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                            <  SRR4302532.4814203/94‑1 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                         >  SRR4302532.3821769/1‑94 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                         >  SRR4302532.4324636/1‑94 (MQ=60)
                                   CGACATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                         <  SRR4302532.5657156/94‑1 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                        <  SRR4302532.2296637/94‑1 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                        >  SRR4302532.4098004/1‑94 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                        >  SRR4302532.1607801/1‑94 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                        >  SRR4302532.2283454/1‑94 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGCAAGTGGCTGAGAATATGACGCACT                                                        >  SRR4302532.451005/1‑94 (MQ=60)
                                      CATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGT                                                      >  SRR4302532.3599499/1‑94 (MQ=60)
                                      CATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGT                                                      <  SRR4302532.2324737/94‑1 (MQ=60)
                                       ATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                     <  SRR4302532.3729615/94‑1 (MQ=60)
                                        TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                    <  SRR4302532.1873404/94‑1 (MQ=60)
                                          ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                  >  SRR4302532.400445/1‑94 (MQ=60)
                                          ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                  >  SRR4302532.4296057/1‑94 (MQ=60)
                                           TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                 >  SRR4302532.1235954/1‑94 (MQ=60)
                                           TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                 <  SRR4302532.5267269/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                                <  SRR4302532.2731201/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCCTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                                <  SRR4302532.251249/94‑1 (MQ=60)
                                             GTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGG                                               >  SRR4302532.2889058/1‑94 (MQ=60)
                                              TCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGC                                              >  SRR4302532.3578351/1‑94 (MQ=60)
                                               CAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCT                                             >  SRR4302532.4037297/1‑94 (MQ=60)
                                                 GGTTTACGCGCAATAACATGGTCGTTTGCGACCTGCGTCAAGACGCCGCATTCGCGGAAGTGGTTGAGAATATGACGCACTGTGGTGCGGCTGA                                           >  SRR4302532.4456547/1‑94 (MQ=60)
                                                 GGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGA                                           >  SRR4302532.5715011/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                          >  SRR4302532.2181080/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                          >  SRR4302532.2687018/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                          <  SRR4302532.1962838/94‑1 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                          >  SRR4302532.669138/1‑94 (MQ=60)
                                                  GTTTACGCACAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                          <  SRR4302532.5864719/94‑1 (MQ=60)
                                                    TTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGT                                        <  SRR4302532.2676761/94‑1 (MQ=60)
                                                    TTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGT                                        <  SRR4302532.172247/94‑1 (MQ=60)
                                                       CGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGT                                     <  SRR4302532.5475426/94‑1 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                    <  SRR4302532.4997609/94‑1 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                    >  SRR4302532.2776610/1‑94 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                    <  SRR4302532.5228359/94‑1 (MQ=60)
                                                         CGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTTTGGTGGGCCTGATGTTGTAC                                   >  SRR4302532.5066640/1‑94 (MQ=60)
                                                         CGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAC                                   >  SRR4302532.5809633/1‑94 (MQ=60)
                                                         CGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAC                                   <  SRR4302532.3169499/94‑1 (MQ=60)
                                                            AATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATT                                >  SRR4302532.5415909/1‑94 (MQ=60)
                                                            AATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATT                                <  SRR4302532.1320141/94‑1 (MQ=60)
                                                            AATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATT                                >  SRR4302532.2139500/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                               >  SRR4302532.5774787/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                               >  SRR4302532.1482330/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                               >  SRR4302532.3233472/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                               <  SRR4302532.1679883/94‑1 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                               >  SRR4302532.3233473/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                               <  SRR4302532.1855780/94‑1 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                             <  SRR4302532.4457542/94‑1 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                             <  SRR4302532.3401780/94‑1 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                            >  SRR4302532.3712123/1‑94 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                            <  SRR4302532.4382122/94‑1 (MQ=60)
                                                                 CATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGC                           >  SRR4302532.3276593/1‑94 (MQ=60)
                                                                  ATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCC                          >  SRR4302532.1492075/1‑94 (MQ=60)
                                                                  ATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCC                          >  SRR4302532.2591102/1‑94 (MQ=60)
                                                                   TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCA                         >  SRR4302532.1704129/1‑94 (MQ=60)
                                                                   TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCA                         >  SRR4302532.1382253/1‑94 (MQ=60)
                                                                     GTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGC                       >  SRR4302532.6114547/1‑94 (MQ=60)
                                                                     GTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGC                       >  SRR4302532.5997950/1‑94 (MQ=60)
                                                                       CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGC                     >  SRR4302532.2348578/1‑94 (MQ=60)
                                                                       CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGC                     >  SRR4302532.2654119/1‑94 (MQ=60)
                                                                        GTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCA                    >  SRR4302532.457159/1‑94 (MQ=60)
                                                                         TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGGCAGCGCAC                   >  SRR4302532.2505926/1‑94 (MQ=60)
                                                                           TCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTT                 <  SRR4302532.4659869/94‑1 (MQ=60)
                                                                            CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT                >  SRR4302532.2711985/1‑94 (MQ=60)
                                                                             CAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG               >  SRR4302532.652717/1‑94 (MQ=60)
                                                                               ACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCG             >  SRR4302532.44107/1‑94 (MQ=60)
                                                                               ACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCG             <  SRR4302532.6322851/94‑1 (MQ=60)
                                                                                CCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA            >  SRR4302532.3014905/1‑94 (MQ=60)
                                                                                 CTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT           >  SRR4302532.3514364/1‑94 (MQ=60)
                                                                                 CTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT           <  SRR4302532.80174/94‑1 (MQ=60)
                                                                                 CTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT           >  SRR4302532.1789360/1‑94 (MQ=60)
                                                                                  TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACCTTGCGAT‑G         <  SRR4302532.6238935/94‑1 (MQ=60)
                                                                                   GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑G       <  SRR4302532.4750114/94‑1 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa      >  SRR4302532.2765597/1‑91 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa      >  SRR4302532.2542609/1‑91 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa      >  SRR4302532.6319999/1‑91 (MQ=60)
                                                                                      TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAG     >  SRR4302532.2613846/1‑94 (MQ=60)
                                                                                      TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GTAAG     <  SRR4302532.6216738/94‑1 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCCCTGTGGTGCGGCTGATGTTGTACATTTTCGCCAGCGCACTTTTCGAT‑Gggagt    >  SRR4302532.6207966/1‑89 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT    <  SRR4302532.3085300/94‑1 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT    <  SRR4302532.2255455/94‑1 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaagg    >  SRR4302532.4156541/1‑89 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaagg    >  SRR4302532.5143720/1‑89 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTAGCTGAGAATATGACGCACTGTGGTGCGGCGGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT    <  SRR4302532.4394194/94‑1 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG   >  SRR4302532.28163/1‑94 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG   >  SRR4302532.5769985/1‑94 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG   >  SRR4302532.5701417/1‑94 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGG  >  SRR4302532.3003306/1‑94 (MQ=60)
                                                                                             |                                                                                          
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑AAGTGG  >  NC_000913/4593161‑4593342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑