Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,051,230	T→C	V54A (GTA→GCA)	sfa →	Sfa

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,051,230	0	T	C	90.1%	629.4 / 48.7	214	V54A (GTA→GCA)	sfa	Sfa
Reads supporting (aligned to +/- strand): ref base T (7/14); new base C (97/95); total (105/109)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 217 total aligned reads displayed.

TCTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCC  >  NC_000913/1051140‑1051319

tcttcAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCAc                                                                                         <  1:1862584/93‑1 (MQ=255)

 cttcAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACTTGACCAGGGCAcc                                                                                        <  1:6381344/93‑1 (MQ=255)

 cttcAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCAcc                                                                                        <  1:2864845/93‑1 (MQ=255)

  ttcAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCg                                                                                       <  2:710275/93‑1 (MQ=255)

   tcAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCgg                                                                                      <  1:2484739/93‑1 (MQ=255)

    cAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCggg                                                                                     >  1:4476787/1‑93 (MQ=255)

     aaCGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGa                                                                                    >  1:1102736/1‑93 (MQ=255)

     aaCGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGa                                                                                    >  1:4506778/1‑93 (MQ=255)

      aCGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGaa                                                                                   >  1:4597823/1‑93 (MQ=255)

      aCGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGaa                                                                                   >  2:3528023/1‑93 (MQ=255)

        gCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAAcc                                                                                 >  2:5615966/1‑93 (MQ=255)

         cTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCa                                                                                >  2:4262408/1‑93 (MQ=255)

          tgtatgtaTAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCaa                                                                               >  2:668410/1‑93 (MQ=255)

           gtatgtatAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAac                                                                              <  1:6188034/93‑1 (MQ=255)

           gtatgtatAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAac                                                                              >  1:1121797/1‑93 (MQ=255)

           gtatgtatAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAac                                                                              <  1:6135794/93‑1 (MQ=255)

              tgtatAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACAcc                                                                           >  1:6516376/1‑93 (MQ=255)

                tataGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCtt                                                                         <  1:50798/93‑1 (MQ=255)

                 ataGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTa                                                                        <  1:2078675/93‑1 (MQ=255)

                  taGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTAc                                                                       >  1:4843929/1‑93 (MQ=255)

                    gTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTg                                                                     <  2:7417/93‑1 (MQ=255)

                    gTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTg                                                                     >  2:1785164/1‑93 (MQ=255)

                     tAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTgg                                                                    <  1:813310/93‑1 (MQ=255)

                        gCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCgt                                                                 >  2:3349290/1‑93 (MQ=255)

                        gCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCgt                                                                 >  1:5819420/1‑93 (MQ=255)

                         cGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCgtg                                                                <  1:1293170/93‑1 (MQ=255)

                         cGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCgtg                                                                <  1:3775283/93‑1 (MQ=255)

                         cGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCgtg                                                                >  1:2031196/1‑93 (MQ=255)

                          gATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCgtgt                                                               <  1:1531776/93‑1 (MQ=255)

                           aTAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGtt                                                              >  2:4753626/1‑93 (MQ=255)

                           aTAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGtt                                                              <  2:6245271/93‑1 (MQ=255)

                            tAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGATCCAACACCTTACTGGCGTGTTg                                                             <  1:5245822/93‑1 (MQ=255)

                            tAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTg                                                             <  2:4951160/93‑1 (MQ=255)

                              aCCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCt                                                           <  2:2425265/93‑1 (MQ=255)

                              aCCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCt                                                           >  2:5092774/1‑93 (MQ=255)

                               ccTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTg                                                          <  2:3370863/93‑1 (MQ=255)

                                cTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGt                                                         >  1:3966700/1‑93 (MQ=255)

                                 tGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTc                                                        >  2:3279796/1‑93 (MQ=255)

                                 tGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTc                                                        >  2:2004670/1‑93 (MQ=255)

                                   ttGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCtt                                                      >  2:2558072/1‑93 (MQ=255)

                                   ttGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCtt                                                      <  2:3470351/93‑1 (MQ=255)

                                     gATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTCCTGGCGTGTTGCTGTCtttt                                                    >  2:1712277/1‑93 (MQ=255)

                                     gATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCtttt                                                    <  2:3268398/93‑1 (MQ=255)

                                       ttattGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTaa                                                  >  2:5221700/1‑93 (MQ=255)

                                       ttattGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTaa                                                  >  2:3676741/1‑93 (MQ=255)

                                        tattGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAg                                                 <  2:6076552/93‑1 (MQ=255)

                                           tGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGAcc                                              <  1:5145058/93‑1 (MQ=255)

                                            gAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCa                                             <  1:4389353/93‑1 (MQ=255)

                                              aTCTTTCGGGGAGATGTCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCaga                                           >  1:6110635/1‑93 (MQ=255)

                                               tCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCagaa                                          <  2:6568576/93‑1 (MQ=255)

                                               tCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCagaa                                          <  1:962500/93‑1 (MQ=255)

                                                cTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCagaag                                         <  1:2572446/93‑1 (MQ=255)

                                                 tttCGGGGAGATGGCTTATAACATTTCTTACCTGACCTGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCagaaga                                        >  2:4658380/1‑93 (MQ=255)

                                                   tCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAgg                                      >  2:5849337/1‑93 (MQ=255)

                                                   tCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAgg                                      <  2:4407994/93‑1 (MQ=255)

                                                     ggggAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGtt                                    >  2:4291704/1‑93 (MQ=255)

                                                      gggAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTa                                   >  1:417740/1‑93 (MQ=255)

                                                      gggAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTa                                   >  2:2992525/1‑93 (MQ=255)

                                                      gggAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTa                                   >  2:4420674/1‑93 (MQ=255)

                                                      gggAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTa                                   <  1:1810410/93‑1 (MQ=255)

                                                       ggAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTaa                                  >  2:4472287/1‑93 (MQ=255)

                                                       ggAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTaa                                  >  2:4144206/1‑93 (MQ=255)

                                                        gagaTGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAAc                                 >  1:3227337/1‑93 (MQ=255)

                                                         agaTGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACa                                <  1:3528814/93‑1 (MQ=255)

                                                          gaTGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAg                               <  1:3074169/93‑1 (MQ=255)

                                                           aTGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGt                              <  1:2261899/93‑1 (MQ=255)

                                                           aTGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGt                              <  1:57516/93‑1 (MQ=255)

                                                            tGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTg                             >  2:1188686/1‑93 (MQ=255)

                                                             ggCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGa                            <  1:669253/93‑1 (MQ=255)

                                                              gCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGaa                           <  1:2011505/93‑1 (MQ=255)

                                                               cTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAat                          >  1:3702350/1‑93 (MQ=255)

                                                                ttATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAata                         <  1:2725548/93‑1 (MQ=255)

                                                                ttATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAata                         >  1:2891088/1‑93 (MQ=255)

                                                                ttATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAata                         >  2:1105017/1‑93 (MQ=255)

                                                                 tataACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAatat                        <  2:58893/93‑1 (MQ=255)

                                                                  ataACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATAtt                       <  2:3602821/93‑1 (MQ=255)

                                                                  ataACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATAtt                       <  1:2532500/93‑1 (MQ=255)

                                                                   taACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTg                      <  2:1974439/93‑1 (MQ=255)

                                                                    aaCATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGa                     >  1:4642482/1‑93 (MQ=255)

                                                                        tttCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAagag                 >  1:1611666/1‑93 (MQ=255)

                                                                        tttCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAagag                 >  2:3780673/1‑93 (MQ=255)

                                                                        tttCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAagag                 <  1:1469508/93‑1 (MQ=255)

                                                                        tttCTTACCTGACCAGGGCACCGGGAACCAAAACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGCTAACAGGGAATATTGAagag                 >  1:1685310/1‑93 (MQ=255)

                                                                          tCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGtt               <  2:5996584/93‑1 (MQ=255)

                                                                            ttACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaa             <  1:5453538/93‑1 (MQ=255)

                                                                             tACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGGTAACAGTGAATATTGAAGAGTTaaa            >  1:5595194/1‑93 (MQ=255)

                                                                              aCCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaa           >  2:5015913/1‑93 (MQ=255)

                                                                              aCCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaa           <  1:3851843/93‑1 (MQ=255)

                                                                              aCCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGACTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaa           >  1:6320529/1‑93 (MQ=255)

                                                                                cTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaaca         >  2:1600605/1‑91 (MQ=255)

                                                                                cTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaaaa         >  2:1273678/1‑93 (MQ=255)

                                                                                cTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaaaa         >  1:986799/1‑93 (MQ=255)

                                                                                cTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaaaa         >  1:4979997/1‑93 (MQ=255)

                                                                                 tGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaaaaa        <  1:328825/93‑1 (MQ=255)

                                                                                   aCCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACa      <  1:4268009/93‑1 (MQ=255)

                                                                                    ccAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTaaaaaaaaaa     >  2:3853396/1‑93 (MQ=255)

                                                                                      aGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGc   >  2:5771511/1‑93 (MQ=255)

                                                                                      aGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGc   <  2:2776871/93‑1 (MQ=255)

                                                                                       gggCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGcc  <  1:2478108/93‑1 (MQ=255)

                                                                                       gggCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGcc  >  1:1386533/1‑93 (MQ=255)

TCTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCC  >  NC_000913/1051140‑1051319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCCGAAA  >  NC_000913/1051140‑1051323

TCTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACC                                                                                            <  SRR4302547.1899350/94‑1 (MQ=60)

 CTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACTTGACCAGGGCACCG                                                                                           <  SRR4302547.6514509/94‑1 (MQ=60)

 CTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCG                                                                                           <  SRR4302547.2038215/94‑1 (MQ=60)

 CTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCG                                                                                           <  SRR4302547.4353799/94‑1 (MQ=60)

 CTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCG                                                                                           <  SRR4302547.2922067/94‑1 (MQ=60)

  TTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGG                                                                                          >  SRR4302547.925915/1‑94 (MQ=60)

   TCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGG                                                                                         <  SRR4302547.2534945/94‑1 (MQ=60)

   TCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGG                                                                                         >  SRR4302547.4217339/1‑94 (MQ=60)

   TCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGG                                                                                         >  SRR4302547.4569274/1‑94 (MQ=60)

    CAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGA                                                                                        >  SRR4302547.1124905/1‑94 (MQ=60)

    CAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGA                                                                                        >  SRR4302547.332464/1‑94 (MQ=60)

    CAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGA                                                                                        >  SRR4302547.4599849/1‑94 (MQ=60)

     AACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAA                                                                                       >  SRR4302547.4692976/1‑94 (MQ=60)

        GCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGAACCGGGAACCA                                                                                    >  SRR4302547.4539435/1‑94 (MQ=60)

          TGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAAC                                                                                  >  SRR4302547.1695867/1‑94 (MQ=60)

          TGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAAC                                                                                  >  SRR4302547.668978/1‑94 (MQ=60)

          TGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAAC                                                                                  >  SRR4302547.1144420/1‑94 (MQ=60)

           GTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACA                                                                                 <  SRR4302547.6316477/94‑1 (MQ=60)

           GTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACA                                                                                 <  SRR4302547.6263079/94‑1 (MQ=60)

            TATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGCACCAACAC                                                                                >  SRR4302547.6245015/1‑94 (MQ=60)

             ATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACC                                                                               >  SRR4302547.6652040/1‑94 (MQ=60)

             ATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACC                                                                               >  SRR4302547.809855/1‑94 (MQ=60)

               GTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTT                                                                             <  SRR4302547.3192452/94‑1 (MQ=60)

                TATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTA                                                                            <  SRR4302547.51745/94‑1 (MQ=60)

                 ATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTAC                                                                           >  SRR4302547.4944309/1‑94 (MQ=60)

                 ATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTAC                                                                           <  SRR4302547.2120125/94‑1 (MQ=60)

                   AGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGCACCAACACCTTACTG                                                                         >  SRR4302547.3579404/1‑94 (MQ=60)

                   AGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTG                                                                         <  SRR4302547.5453838/94‑1 (MQ=60)

                   AGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTG                                                                         <  SRR4302547.5669687/94‑1 (MQ=60)

                     TAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGC                                                                       <  SRR4302547.828754/94‑1 (MQ=60)

                       AGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGT                                                                     >  SRR4302547.5939819/1‑94 (MQ=60)

                        GCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTG                                                                    >  SRR4302547.2071648/1‑94 (MQ=60)

                         CGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGT                                                                   <  SRR4302547.3852277/94‑1 (MQ=60)

                         CGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGT                                                                   <  SRR4302547.1319196/94‑1 (MQ=60)

                         CGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGT                                                                   <  SRR4302547.4696134/94‑1 (MQ=60)

                          GATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTT                                                                  <  SRR4302547.1562269/94‑1 (MQ=60)

                          GATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTT                                                                  <  SRR4302547.3821668/94‑1 (MQ=60)

                          GATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTT                                                                  <  SRR4302547.511074/94‑1 (MQ=60)

                          GATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTT                                                                  <  SRR4302547.3913726/94‑1 (MQ=60)

                            TAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGATCCAACACCTTACTGGCGTGTTGC                                                                <  SRR4302547.5353793/94‑1 (MQ=60)

                              ACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTG                                                              >  SRR4302547.3688024/1‑94 (MQ=60)

                               CCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGT                                                             >  SRR4302547.4047377/1‑94 (MQ=60)

                                CTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTC                                                            <  SRR4302547.6360832/94‑1 (MQ=60)

                                     GATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTA                                                       <  SRR4302547.2652896/94‑1 (MQ=60)

                                     GATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTA                                                       <  SRR4302547.641471/94‑1 (MQ=60)

                                     GATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTA                                                       >  SRR4302547.2058981/1‑94 (MQ=60)

                                       TTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAG                                                     <  SRR4302547.526662/94‑1 (MQ=60)

                                        TATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGA                                                    >  SRR4302547.1575881/1‑94 (MQ=60)

                                          TTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACC                                                  <  SRR4302547.1855267/94‑1 (MQ=60)

                                           TGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCA                                                 <  SRR4302547.5251033/94‑1 (MQ=60)

                                            GAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAG                                                <  SRR4302547.4479671/94‑1 (MQ=60)

                                             AATCTTTCGGGGAGATGTCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGA                                               >  SRR4302547.6237388/1‑94 (MQ=60)

                                             AATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGA                                               <  SRR4302547.6561710/94‑1 (MQ=60)

                                              ATCTTTCGGGGAGATGGCTTATAACATTTCTTGCCTGACCAGGGCACCGGGAGCCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAA                                              <  SRR4302547.1619786/94‑1 (MQ=60)

                                               TCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAG                                             <  SRR4302547.981195/94‑1 (MQ=60)

                                                CTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGA                                            <  SRR4302547.2624384/94‑1 (MQ=60)

                                                CTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGA                                            >  SRR4302547.6274668/1‑94 (MQ=60)

                                                 TTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAG                                           >  SRR4302547.1836468/1‑94 (MQ=60)

                                                 TTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAG                                           <  SRR4302547.4860453/94‑1 (MQ=60)

                                                 TTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAG                                           <  SRR4302547.2939557/94‑1 (MQ=60)

                                                 TTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAG                                           <  SRR4302547.3934236/94‑1 (MQ=60)

                                                  TTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGG                                          >  SRR4302547.1129647/1‑94 (MQ=60)

                                                    CGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTT                                        <  SRR4302547.346305/94‑1 (MQ=60)

                                                     GGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTA                                       >  SRR4302547.425484/1‑94 (MQ=60)

                                                      GGGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAA                                      <  SRR4302547.1846180/94‑1 (MQ=60)

                                                       GGAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAAC                                     >  SRR4302547.3291749/1‑94 (MQ=60)

                                                        GAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACA                                    <  SRR4302547.1249246/94‑1 (MQ=60)

                                                        GAGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACA                                    <  SRR4302547.3046162/94‑1 (MQ=60)

                                                         AGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAG                                   <  SRR4302547.3600862/94‑1 (MQ=60)

                                                         AGATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAG                                   >  SRR4302547.1452814/1‑94 (MQ=60)

                                                          GATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGT                                  <  SRR4302547.3135239/94‑1 (MQ=60)

                                                           ATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTG                                 <  SRR4302547.2307598/94‑1 (MQ=60)

                                                           ATGGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTG                                 <  SRR4302547.58605/94‑1 (MQ=60)

                                                             GGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAA                               <  SRR4302547.681569/94‑1 (MQ=60)

                                                             GGCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAA                               <  SRR4302547.5790327/94‑1 (MQ=60)

                                                              GCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAAT                              <  SRR4302547.2051529/94‑1 (MQ=60)

                                                              GCTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAAT                              >  SRR4302547.3777919/1‑94 (MQ=60)

                                                               CTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATA                             >  SRR4302547.4384723/1‑94 (MQ=60)

                                                               CTTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATA                             >  SRR4302547.2948752/1‑94 (MQ=60)

                                                                TTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATAT                            <  SRR4302547.2115738/94‑1 (MQ=60)

                                                                TTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATAT                            >  SRR4302547.496724/1‑94 (MQ=60)

                                                                TTATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATAT                            <  SRR4302547.2780272/94‑1 (MQ=60)

                                                                  ATAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTG                          <  SRR4302547.2583690/94‑1 (MQ=60)

                                                                   TAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGA                         <  SRR4302547.4829808/94‑1 (MQ=60)

                                                                   TAACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGA                         >  SRR4302547.4738632/1‑94 (MQ=60)

                                                                    AACATTTCTTACCTGACCAGGGCACCGGGAACCAACTCCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAA                        <  SRR4302547.961538/94‑1 (MQ=60)

                                                                    AACATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAA                        >  SRR4302547.2082850/1‑94 (MQ=60)

                                                                      CATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGA                      >  SRR4302547.2533611/1‑94 (MQ=60)

                                                                       ATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAG                     >  SRR4302547.1643594/1‑94 (MQ=60)

                                                                       ATTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAG                     <  SRR4302547.2316603/94‑1 (MQ=60)

                                                                       ATTTCTTACCTGACCAGGGCACCGGGAACCAAAACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGCTAACAGGGAATATTGAAGAG                     >  SRR4302547.1718478/1‑94 (MQ=60)

                                                                        TTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGT                    <  SRR4302547.1498869/94‑1 (MQ=60)

                                                                        TTTCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGT                    >  SRR4302547.2855338/1‑94 (MQ=60)

                                                                          TCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTA                  <  SRR4302547.1551994/94‑1 (MQ=60)

                                                                          TCTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTA                  <  SRR4302547.4588440/94‑1 (MQ=60)

                                                                           CTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAA                 <  SRR4302547.2736310/94‑1 (MQ=60)

                                                                           CTTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGGTAA                 >  SRR4302547.4673804/1‑94 (MQ=60)

                                                                            TTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAA                <  SRR4302547.5566224/94‑1 (MQ=60)

                                                                            TTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAA                >  SRR4302547.2544215/1‑94 (MQ=60)

                                                                            TTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAA                >  SRR4302547.2250210/1‑94 (MQ=60)

                                                                            TTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGGTAACAGTGAATATTGAAGAGTTAAA                >  SRR4302547.5710615/1‑94 (MQ=60)

                                                                            TTACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTCTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAA                >  SRR4302547.5936270/1‑94 (MQ=60)

                                                                             TACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAA               <  SRR4302547.4323450/94‑1 (MQ=60)

                                                                             TACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGACTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAA               >  SRR4302547.6451950/1‑94 (MQ=60)

                                                                              ACCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAA              <  SRR4302547.3930248/94‑1 (MQ=60)

                                                                               CCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAA             >  SRR4302547.5082995/1‑94 (MQ=60)

                                                                               CCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAA             >  SRR4302547.1006005/1‑94 (MQ=60)

                                                                               CCTGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGGTAAAAAA             >  SRR4302547.1400797/1‑94 (MQ=60)

                                                                                 TGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAAC           <  SRR4302547.2585417/94‑1 (MQ=60)

                                                                                 TGACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAAA           <  SRR4302547.334945/94‑1 (MQ=60)

                                                                                  GACCAGGGTACCGGGACCCACCACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACA          <  SRR4302547.6453945/94‑1 (MQ=60)

                                                                                   ACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAA         >  SRR4302547.5237169/1‑94 (MQ=60)

                                                                                   ACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAA         >  SRR4302547.6117253/1‑94 (MQ=60)

                                                                                   ACCAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAA         <  SRR4302547.4355539/94‑1 (MQ=60)

                                                                                     CAGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGC       >  SRR4302547.1002410/1‑94 (MQ=60)

                                                                                      AGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCC      >  SRR4302547.564476/1‑94 (MQ=60)

                                                                                      AGGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCC      >  SRR4302547.1414289/1‑94 (MQ=60)

                                                                                       GGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCCG     >  SRR4302547.2018996/1‑94 (MQ=60)

                                                                                       GGGCACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCCG     <  SRR4302547.2528201/94‑1 (MQ=60)

                                                                                          CACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCCGAAA  >  SRR4302547.3744227/1‑94 (MQ=60)

                                                                                          CACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCCGAAA  >  SRR4302547.340007/1‑94 (MQ=60)

TCTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCTTTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTACTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGAAGAGTTAAAAAAACAAGCCGAAA  >  NC_000913/1051140‑1051323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`