Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,425,052 C→T A192V (GCT→GTT)  b1368 → putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,425,0520CT85.6% 647.2 / 85.9 251A192V (GCT→GTT) b1368putative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base C (21/15);  new base T (101/113);  total (123/128)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-03

Only 100 of 254 total aligned reads displayed.

GCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGC  >  NC_000913/1424961‑1425142
                                                                                           |                                                                                          
 ctgcGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTc                                                                                          <  1:6464241/93‑1 (MQ=255)
  tgcGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCa                                                                                         >  2:3175203/1‑93 (MQ=255)
   gcGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAg                                                                                        <  1:361267/93‑1 (MQ=255)
     gTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGc                                                                                      <  2:3005161/93‑1 (MQ=255)
     gTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGc                                                                                      <  1:4320861/93‑1 (MQ=255)
     gTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGc                                                                                      >  1:2482113/1‑93 (MQ=255)
      tttGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCa                                                                                     >  2:5271752/1‑93 (MQ=255)
      tttGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCa                                                                                     <  2:4155661/93‑1 (MQ=255)
      tttGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCa                                                                                     >  1:2271782/1‑93 (MQ=255)
      tttGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCa                                                                                     >  2:5767897/1‑93 (MQ=255)
        tGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCaga                                                                                   >  2:4462222/1‑93 (MQ=255)
        tGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCaga                                                                                   <  1:2085848/93‑1 (MQ=255)
        tGCATCAGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCaga                                                                                   <  1:4034393/93‑1 (MQ=255)
           aTCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCg                                                                                >  2:1639822/1‑93 (MQ=255)
            tCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCga                                                                               <  1:4416628/93‑1 (MQ=255)
            tCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCga                                                                               >  1:6327580/1‑93 (MQ=255)
              cGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCgaga                                                                             >  1:4046084/1‑93 (MQ=255)
               gggAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGaa                                                                            <  2:3868729/93‑1 (MQ=255)
                  aaaaTAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAagca                                                                         >  2:3116521/1‑93 (MQ=255)
                  aaaaTAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAagca                                                                         <  2:5790798/93‑1 (MQ=255)
                  aaaaTAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAagca                                                                         <  2:4836968/93‑1 (MQ=255)
                   aaataaatGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAagcag                                                                        <  2:5357912/93‑1 (MQ=255)
                    aataaatGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGAGAGAagcagc                                                                       >  2:3069231/1‑93 (MQ=255)
                     ataaatGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAgcagca                                                                      <  2:1583921/93‑1 (MQ=255)
                      taaatGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCaa                                                                     >  2:3872855/1‑93 (MQ=255)
                      taaatGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCaa                                                                     >  2:3960950/1‑93 (MQ=255)
                        aatGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCaaaa                                                                   >  2:1042376/1‑93 (MQ=255)
                         atGGTTCTCAGTTTGAGAATTATAGCCGAAGAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAg                                                                  <  2:5180607/93‑1 (MQ=255)
                         atGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCaaaac                                                                  >  1:3228064/1‑92 (MQ=255)
                         atGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAg                                                                  <  2:398299/93‑1 (MQ=255)
                          tGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAgc                                                                 <  1:920497/93‑1 (MQ=255)
                          tGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAgc                                                                 <  2:2612252/93‑1 (MQ=255)
                            gTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAgcgc                                                               >  2:5882710/1‑93 (MQ=255)
                             ttCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCa                                                              <  2:1897586/93‑1 (MQ=255)
                             ttCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCa                                                              <  1:3154856/93‑1 (MQ=255)
                              tctcAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCat                                                             <  2:5927050/93‑1 (MQ=255)
                               ctcAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCatg                                                            <  2:703104/93‑1 (MQ=255)
                                tcAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCatga                                                           <  1:1954379/93‑1 (MQ=255)
                                 cAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCatgat                                                          <  2:2102792/93‑1 (MQ=255)
                                 cAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCatgat                                                          >  1:5099443/1‑93 (MQ=255)
                                   gTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCAtgatga                                                        >  1:1098610/1‑93 (MQ=255)
                                     ttGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCAtgatgatc                                                      <  1:5277868/93‑3 (MQ=255)
                                     ttGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAAc                                                      >  2:2158418/1‑93 (MQ=255)
                                        agaATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAgg                                                   >  1:6165179/1‑93 (MQ=255)
                                         aaaTTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGgt                                                  <  1:4377102/92‑1 (MQ=255)
                                           aTTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGgttg                                                >  1:1341146/1‑93 (MQ=255)
                                           aTTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGgttg                                                <  2:3413124/93‑1 (MQ=255)
                                           aTTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGgttg                                                <  1:58702/93‑1 (MQ=255)
                                           aTTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGgttg                                                >  1:2744710/1‑93 (MQ=255)
                                            ttATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGgttgt                                               >  1:3473657/1‑93 (MQ=255)
                                              ataGCGGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGttgttg                                             >  1:4206206/1‑93 (MQ=255)
                                               taGCCTAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGc                                            <  2:4657331/93‑1 (MQ=255)
                                               taGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGc                                            <  1:162554/93‑1 (MQ=255)
                                               taGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGc                                            >  1:3009821/1‑93 (MQ=255)
                                                aGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCt                                           <  2:143200/93‑1 (MQ=255)
                                                 gCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCtt                                          >  1:5924694/1‑93 (MQ=255)
                                                  ccGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCttt                                         <  1:4622738/93‑1 (MQ=255)
                                                  ccGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCttg                                         >  1:5330016/1‑92 (MQ=255)
                                                    gAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGc                                       <  1:3181098/93‑1 (MQ=255)
                                                     aaaaaTACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCa                                      >  2:831920/1‑93 (MQ=255)
                                                     aaaaaTACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCa                                      <  1:1149511/93‑1 (MQ=255)
                                                         aTACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGt                                  >  1:5071119/1‑93 (MQ=255)
                                                         aTACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGt                                  >  1:3710536/1‑93 (MQ=255)
                                                          tACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTc                                 <  2:3265777/93‑1 (MQ=255)
                                                           aCAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCt                                <  2:5353623/93‑1 (MQ=255)
                                                             aGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGa                              <  2:92939/93‑1 (MQ=255)
                                                              ggAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAt                             <  2:2564304/93‑1 (MQ=255)
                                                              ggAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAt                             <  2:2473394/93‑1 (MQ=255)
                                                              ggAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAt                             >  1:6508332/1‑93 (MQ=255)
                                                               gAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAtt                            <  2:6310984/93‑1 (MQ=255)
                                                               gAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAtt                            <  2:54587/93‑1 (MQ=255)
                                                                aaaCACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAttc                           >  1:4695150/1‑92 (MQ=255)
                                                                aaaCACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTg                           <  2:2355607/93‑1 (MQ=255)
                                                                 aaCACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGc                          <  2:6365955/93‑1 (MQ=255)
                                                                  acacGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCt                         >  1:2625365/1‑93 (MQ=255)
                                                                  acacGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCt                         >  2:4598725/1‑93 (MQ=255)
                                                                   cacGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTc                        >  2:6500756/1‑93 (MQ=255)
                                                                   cacGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTc                        >  1:1692541/1‑93 (MQ=255)
                                                                    acGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCa                       <  1:1200536/93‑1 (MQ=255)
                                                                     cGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCaa                      >  1:4788601/1‑93 (MQ=255)
                                                                        cGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACtt                   <  1:1507527/93‑1 (MQ=255)
                                                                          agagCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGa                 >  1:2208898/1‑93 (MQ=255)
                                                                           gagCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGAt                >  2:2716623/1‑93 (MQ=255)
                                                                            agCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATc               >  1:3442737/1‑93 (MQ=255)
                                                                             gCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATcc              <  1:1459085/93‑1 (MQ=255)
                                                                             gCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATcc              <  2:1772852/93‑1 (MQ=255)
                                                                             gCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATcc              <  1:555292/93‑1 (MQ=255)
                                                                              cTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCt             <  1:3572647/93‑1 (MQ=255)
                                                                               ttACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTg            <  1:2503322/93‑1 (MQ=255)
                                                                               ttACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTg            <  1:3922002/93‑1 (MQ=255)
                                                                                tACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGt           >  1:3838856/1‑93 (MQ=255)
                                                                                 aCCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTc          <  1:1244743/93‑1 (MQ=255)
                                                                                 aCCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTc          <  2:6443620/93‑1 (MQ=255)
                                                                                  ccGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCg         <  2:1120359/93‑1 (MQ=255)
                                                                                  ccGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCg         >  2:1404840/1‑93 (MQ=255)
                                                                                   cGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCgg        <  2:5027606/93‑1 (MQ=255)
                                                                                     gagaGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGaa      >  1:410274/1‑93 (MQ=255)
                                                                                     gagaGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGaa      >  2:4225747/1‑93 (MQ=255)
                                                                                       gagGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACt    >  1:4112844/1‑93 (MQ=255)
                                                                                         ggCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGc  >  1:564509/1‑93 (MQ=255)
                                                                                           |                                                                                          
GCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGC  >  NC_000913/1424961‑1425142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGCTTT  >  NC_000913/1424959‑1425145
                                                                                             |                                                                                             
ATGCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGT                                                                                               <  SRR4302547.2886235/94‑1 (MQ=60)
 TGCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTT                                                                                              <  SRR4302547.3532586/94‑1 (MQ=60)
 TGCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTT                                                                                              >  SRR4302547.1895917/1‑94 (MQ=60)
  GCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTC                                                                                             <  SRR4302547.3767802/94‑1 (MQ=60)
  GCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTC                                                                                             >  SRR4302547.452266/1‑94 (MQ=60)
   CTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCA                                                                                            <  SRR4302547.1723286/94‑1 (MQ=60)
   CTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCA                                                                                            <  SRR4302547.3271096/94‑1 (MQ=60)
   CTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCA                                                                                            <  SRR4302547.383772/94‑1 (MQ=60)
   CTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCA                                                                                            <  SRR4302547.6598847/94‑1 (MQ=60)
     GCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGG                                                                                          <  SRR4302547.367975/94‑1 (MQ=60)
      CGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGC                                                                                         >  SRR4302547.2532275/1‑94 (MQ=60)
      CGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGC                                                                                         >  SRR4302547.1566080/1‑94 (MQ=60)
      CGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGC                                                                                         >  SRR4302547.6617506/1‑94 (MQ=60)
       GTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCA                                                                                        >  SRR4302547.2317733/1‑94 (MQ=60)
       GTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCA                                                                                        <  SRR4302547.4409809/94‑1 (MQ=60)
        TTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAG                                                                                       >  SRR4302547.5350476/1‑94 (MQ=60)
         TTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGA                                                                                      <  SRR4302547.37016/94‑1 (MQ=60)
         TTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGA                                                                                      >  SRR4302547.6783589/1‑94 (MQ=60)
          TGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAG                                                                                     <  SRR4302547.2127447/94‑1 (MQ=60)
          TGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAG                                                                                     >  SRR4302547.4096117/1‑94 (MQ=60)
          TGCATCAGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAG                                                                                     <  SRR4302547.4116311/94‑1 (MQ=60)
           TCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGC                                                                                    <  SRR4302547.6518001/94‑1 (MQ=60)
           GCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGC                                                                                    >  SRR4302547.33009/1‑94 (MQ=60)
            CATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCG                                                                                   >  SRR4302547.5102568/1‑94 (MQ=60)
            CATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCG                                                                                   >  SRR4302547.3572776/1‑94 (MQ=60)
            CATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCG                                                                                   <  SRR4302547.2033805/94‑1 (MQ=60)
             ATCCGGGAAAATAAATGTTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGA                                                                                  <  SRR4302547.4477891/94‑1 (MQ=60)
             ATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGA                                                                                  >  SRR4302547.4844355/1‑94 (MQ=60)
             ATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGA                                                                                  >  SRR4302547.6459170/1‑94 (MQ=60)
              TCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAG                                                                                 <  SRR4302547.4507759/94‑1 (MQ=60)
              TCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAG                                                                                 <  SRR4302547.2447724/94‑1 (MQ=60)
              TCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAG                                                                                 >  SRR4302547.4448138/1‑94 (MQ=60)
               CCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGA                                                                                >  SRR4302547.4128240/1‑94 (MQ=60)
                CGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAA                                                                               >  SRR4302547.5329605/1‑94 (MQ=60)
                  GGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGC                                                                             >  SRR4302547.129689/1‑94 (MQ=60)
                     AAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGC                                                                          >  SRR4302547.1017864/1‑94 (MQ=60)
                     AAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGC                                                                          >  SRR4302547.5423829/1‑94 (MQ=60)
                      AATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCA                                                                         <  SRR4302547.1509824/94‑1 (MQ=60)
                       ATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTCCCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAA                                                                        <  SRR4302547.123529/94‑1 (MQ=60)
                         AAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAA                                                                      >  SRR4302547.1797117/1‑94 (MQ=60)
                          AATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAG                                                                     <  SRR4302547.476139/94‑1 (MQ=60)
                          AATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAG                                                                     <  SRR4302547.2306676/94‑1 (MQ=60)
                          AATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAC                                                                     >  SRR4302547.3292490/1‑94 (MQ=60)
                           ATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGC                                                                    >  SRR4302547.2704381/1‑94 (MQ=60)
                           ATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGC                                                                    >  SRR4302547.2185198/1‑94 (MQ=60)
                           ATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGC                                                                    <  SRR4302547.6182304/94‑1 (MQ=60)
                            TGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCG                                                                   <  SRR4302547.938272/94‑1 (MQ=60)
                               TTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCAT                                                                >  SRR4302547.1352441/1‑94 (MQ=60)
                               TTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCAT                                                                <  SRR4302547.3217593/94‑1 (MQ=60)
                               TTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCAT                                                                <  SRR4302547.112195/94‑1 (MQ=60)
                                 CTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGA                                                              >  SRR4302547.3453671/1‑94 (MQ=60)
                                  TCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGAT                                                             >  SRR4302547.5204585/1‑94 (MQ=60)
                                  TCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGAT                                                             <  SRR4302547.1993155/94‑1 (MQ=60)
                                   CAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATG                                                            >  SRR4302547.4186485/1‑94 (MQ=60)
                                    AGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGA                                                           >  SRR4302547.1120670/1‑94 (MQ=60)
                                     GTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAA                                                          >  SRR4302547.4250787/1‑94 (MQ=60)
                                     GTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAA                                                          >  SRR4302547.4798643/1‑94 (MQ=60)
                                       TTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGATCA                                                        <  SRR4302547.5386714/94‑1 (MQ=60)
                                       TTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACA                                                        >  SRR4302547.6042239/1‑94 (MQ=60)
                                         GAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGG                                                      >  SRR4302547.6293143/1‑94 (MQ=60)
                                           AAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTT                                                    <  SRR4302547.4467063/94‑1 (MQ=60)
                                            AATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTG                                                   >  SRR4302547.3231878/1‑94 (MQ=60)
                                            AATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTG                                                   >  SRR4302547.1368090/1‑94 (MQ=60)
                                            AATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTG                                                   >  SRR4302547.2799759/1‑94 (MQ=60)
                                             ATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGT                                                  >  SRR4302547.3544500/1‑94 (MQ=60)
                                             ATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGT                                                  <  SRR4302547.59805/94‑1 (MQ=60)
                                             ATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGGTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGATGT                                                  >  SRR4302547.5194913/1‑94 (MQ=60)
                                              TTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTT                                                 <  SRR4302547.2926214/94‑1 (MQ=60)
                                              TTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTT                                                 <  SRR4302547.5445719/94‑1 (MQ=60)
                                              TTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTT                                                 >  SRR4302547.2083863/1‑94 (MQ=60)
                                              TTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGGTCAGGCAGATCGAGAACCAGCAAAAGCGCATGATGAACAGGTTGTT                                                 >  SRR4302547.3301371/1‑94 (MQ=60)
                                               TATAGCGGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTG                                                >  SRR4302547.4292036/1‑94 (MQ=60)
                                               TATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTG                                                >  SRR4302547.4464753/1‑94 (MQ=60)
                                                ATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGC                                               >  SRR4302547.3069671/1‑94 (MQ=60)
                                                 TGGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCT                                              <  SRR4302547.1529538/94‑1 (MQ=60)
                                                 TAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCT                                              <  SRR4302547.6253046/94‑1 (MQ=60)
                                                 TAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCT                                              <  SRR4302547.165534/94‑1 (MQ=60)
                                                  AGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTT                                             >  SRR4302547.6047491/1‑94 (MQ=60)
                                                  AGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTT                                             >  SRR4302547.40668/1‑94 (MQ=60)
                                                   GCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTG                                            >  SRR4302547.5439880/1‑94 (MQ=60)
                                                    CCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTG                                           <  SRR4302547.3933923/94‑1 (MQ=60)
                                                    CCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTG                                           <  SRR4302547.4718463/94‑1 (MQ=60)
                                                      GAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCA                                         <  SRR4302547.3244496/94‑1 (MQ=60)
                                                       AAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAA                                        >  SRR4302547.5591292/1‑94 (MQ=60)
                                                       AAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAA                                        <  SRR4302547.1172754/94‑1 (MQ=60)
                                                       AAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAA                                        >  SRR4302547.5774550/1‑94 (MQ=60)
                                                          AATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGT                                     >  SRR4302547.5175747/1‑94 (MQ=60)
                                                          AATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGT                                     >  SRR4302547.3786284/1‑94 (MQ=60)
                                                           ATACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTC                                    <  SRR4302547.2538654/94‑1 (MQ=60)
                                                            TACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCT                                   <  SRR4302547.5669902/94‑1 (MQ=60)
                                                            TACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCT                                   >  SRR4302547.3184536/1‑94 (MQ=60)
                                                            TACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCT                                   <  SRR4302547.3647646/94‑1 (MQ=60)
                                                            TACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCT                                   >  SRR4302547.1214978/1‑94 (MQ=60)
                                                            TACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCT                                   <  SRR4302547.3527991/94‑1 (MQ=60)
                                                             ACAGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTG                                  >  SRR4302547.4171329/1‑94 (MQ=60)
                                                             ACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTTCTTTGCAACGTCTG                                  <  SRR4302547.4287189/94‑1 (MQ=60)
                                                               AGGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAT                                >  SRR4302547.6643842/1‑94 (MQ=60)
                                                               AGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGAT                                >  SRR4302547.1459244/1‑94 (MQ=60)
                                                                GGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATT                               >  SRR4302547.249010/1‑94 (MQ=60)
                                                                GGAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATT                               >  SRR4302547.3230819/1‑94 (MQ=60)
                                                                 GAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTG                              <  SRR4302547.633385/94‑1 (MQ=60)
                                                                 GAAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTC                              >  SRR4302547.4792401/1‑94 (MQ=60)
                                                                  AAACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGC                             >  SRR4302547.4151778/1‑94 (MQ=60)
                                                                   AACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCT                            >  SRR4302547.2678250/1‑94 (MQ=60)
                                                                    ACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGGGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTC                           >  SRR4302547.1674072/1‑94 (MQ=60)
                                                                    ACACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTC                           >  SRR4302547.1725838/1‑94 (MQ=60)
                                                                      ACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAA                         >  SRR4302547.4887817/1‑94 (MQ=60)
                                                                      ACGGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAA                         <  SRR4302547.1224769/94‑1 (MQ=60)
                                                                        GGCGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACT                       <  SRR4302547.3603867/94‑1 (MQ=60)
                                                                          CGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTG                     <  SRR4302547.1537573/94‑1 (MQ=60)
                                                                          CGAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTG                     <  SRR4302547.1176772/94‑1 (MQ=60)
                                                                           GAGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGA                    >  SRR4302547.439001/1‑94 (MQ=60)
                                                                           GAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGA                    >  SRR4302547.2253311/1‑94 (MQ=60)
                                                                            AGAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGAT                   <  SRR4302547.4121238/94‑1 (MQ=60)
                                                                             GAGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATC                  >  SRR4302547.3512929/1‑94 (MQ=60)
                                                                              AGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCC                 >  SRR4302547.952702/1‑94 (MQ=60)
                                                                              AGCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCC                 >  SRR4302547.2592560/1‑94 (MQ=60)
                                                                               GCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCT                <  SRR4302547.565292/94‑1 (MQ=60)
                                                                               GCTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCT                <  SRR4302547.1488242/94‑1 (MQ=60)
                                                                                CTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTG               <  SRR4302547.3645535/94‑1 (MQ=60)
                                                                                CTTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTG               >  SRR4302547.4996804/1‑94 (MQ=60)
                                                                                 TTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGT              <  SRR4302547.4001749/94‑1 (MQ=60)
                                                                                 TTACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGT              <  SRR4302547.2553910/94‑1 (MQ=60)
                                                                                 TTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGT              >  SRR4302547.2894441/1‑94 (MQ=60)
                                                                                 TTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGT              >  SRR4302547.3917046/1‑94 (MQ=60)
                                                                                  TACCGGAGAGGTTCAGGCAGAGCGAGACGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTC             >  SRR4302547.2269981/1‑94 (MQ=60)
                                                                                  TACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTC             <  SRR4302547.6525479/94‑1 (MQ=60)
                                                                                   ACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCG            <  SRR4302547.5890760/94‑1 (MQ=60)
                                                                                   ACCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCG            <  SRR4302547.1269866/94‑1 (MQ=60)
                                                                                   ACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCG            <  SRR4302547.2639717/94‑1 (MQ=60)
                                                                                    CCGGAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGG           <  SRR4302547.4577297/94‑1 (MQ=60)
                                                                                      GGAGAGGTTAAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAA         >  SRR4302547.6602444/1‑94 (MQ=60)
                                                                                      GGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAA         >  SRR4302547.417879/1‑94 (MQ=60)
                                                                                       GAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAAC        >  SRR4302547.3695571/1‑94 (MQ=60)
                                                                                       GAGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAAC        <  SRR4302547.3842918/94‑1 (MQ=60)
                                                                                        AGAGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACT       >  SRR4302547.4196431/1‑94 (MQ=60)
                                                                                          AGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGC     >  SRR4302547.2543873/1‑94 (MQ=60)
                                                                                          AGGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGC     >  SRR4302547.6735458/1‑94 (MQ=60)
                                                                                          AGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGC     >  SRR4302547.574665/1‑94 (MQ=60)
                                                                                           GGTTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGCT    >  SRR4302547.551848/1‑94 (MQ=60)
                                                                                             TTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGCTTT  <  SRR4302547.6621890/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATGCTGCGTTTGCATCCGGGAAAATAAATGGTTCTCAGTTTGAGAATTATAGCCGAAAAATACAGGAAACACGGCGAGAGCTTACCGGAGAGGCTCAGGCAGAGCGAGAAGCAGCAAAAGCGCATGATGAACAGGTTGTTGCTTTGCAACGTCTGATTGCTCAACTTGATCCTGTCGGAACTGCTTT  >  NC_000913/1424959‑1425145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑