Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,754,991	A→G	T424A (ACT→GCT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,754,991	0	A	G	83.9%	520.6 / 88.2	211	T424A (ACT→GCT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (21/13); new base G (77/100); total (98/113)
Fisher's exact test for biased strand distribution p-value = 6.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 214 total aligned reads displayed.

GCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  >  NC_000913/1754901‑1755082

gCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCt                                                                                           <  2:3398926/93‑1 (MQ=255)

 ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCtt                                                                                          >  1:2159025/1‑93 (MQ=255)

  caccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTc                                                                                         <  1:1711867/93‑1 (MQ=255)

   accaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCt                                                                                        <  2:4477818/93‑1 (MQ=255)

   accaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCt                                                                                        >  1:6534312/1‑93 (MQ=255)

     caTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTAc                                                                                      <  2:5034584/93‑1 (MQ=255)

     caTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTAc                                                                                      <  2:2654735/93‑1 (MQ=255)

      aTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACt                                                                                     <  2:1630715/93‑1 (MQ=255)

      aTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACt                                                                                     >  1:307672/1‑93 (MQ=255)

       tCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGTCGCAGCTTGTTAAAGAAATCGCTTCTACtg                                                                                    >  2:1927051/1‑93 (MQ=255)

        ccTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtga                                                                                   <  1:5550678/93‑1 (MQ=255)

         cTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACtgat                                                                                  <  1:877058/93‑1 (MQ=255)

          tGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgatg                                                                                 >  2:2863299/1‑93 (MQ=255)

           ggCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACtgatga                                                                                <  2:5047703/93‑1 (MQ=255)

            gCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTgatgat                                                                               >  1:4577240/1‑93 (MQ=255)

             cACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGAtt                                                                              >  1:6347617/1‑93 (MQ=255)

             cACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGAtt                                                                              <  2:1832153/93‑1 (MQ=255)

              aCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGAttt                                                                             >  2:2025945/1‑93 (MQ=255)

                tGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCt                                                                           <  1:791161/93‑1 (MQ=255)

                 gACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTa                                                                          >  1:6122529/1‑93 (MQ=255)

                   ccaccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTAcc                                                                        <  2:5171301/93‑1 (MQ=255)

                    caccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCg                                                                       <  2:6568856/93‑1 (MQ=255)

                      ccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTc                                                                     <  1:2927692/93‑1 (MQ=255)

                      ccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTc                                                                     <  1:6426734/93‑1 (MQ=255)

                      ccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTc                                                                     >  2:1857622/1‑93 (MQ=255)

                       caACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCt                                                                    <  2:4169431/93‑1 (MQ=255)

                         aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTgg                                                                  >  1:3977906/1‑93 (MQ=255)

                          cGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTggg                                                                 <  2:6007956/93‑1 (MQ=255)

                          cGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTggg                                                                 <  1:5798104/93‑1 (MQ=255)

                           gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGt                                                                <  2:1716131/93‑1 (MQ=255)

                           gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGt                                                                >  1:621991/1‑93 (MQ=255)

                           gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGt                                                                >  1:1786190/1‑93 (MQ=255)

                            aaaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTa                                                               <  2:492772/93‑1 (MQ=255)

                             aaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTaa                                                              >  1:4598522/1‑93 (MQ=255)

                              aaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTaaa                                                             >  2:1064595/1‑93 (MQ=255)

                               aaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAg                                                            <  2:2419284/93‑1 (MQ=255)

                                aaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGa                                                           <  2:2576984/93‑1 (MQ=255)

                                 aCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGaa                                                          >  1:4048626/1‑93 (MQ=255)

                                  cGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAAc                                                         <  1:3807947/93‑1 (MQ=255)

                                   ggCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACt                                                        <  1:2017716/93‑1 (MQ=255)

                                   ggCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACt                                                        <  1:2629118/93‑1 (MQ=255)

                                   ggCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACt                                                        <  1:3992543/93‑1 (MQ=255)

                                   ggCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACt                                                        <  2:4855731/93‑1 (MQ=255)

                                     cTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTgg                                                      <  1:23161/93‑1 (MQ=255)

                                      tcatcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGc                                                     >  1:5895187/1‑93 (MQ=255)

                                        atcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctc                                                   <  1:2431179/93‑1 (MQ=255)

                                        atcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctc                                                   >  2:2098471/1‑93 (MQ=255)

                                           aGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGATATCGCTTCTTCTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGc                                                <  1:3299714/93‑1 (MQ=255)

                                            gTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                                               >  2:1150650/1‑93 (MQ=255)

                                            gTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                                               >  1:2961264/1‑93 (MQ=255)

                                            gTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                                               <  2:4034275/93‑1 (MQ=255)

                                            gTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                                               <  2:2494758/93‑1 (MQ=255)

                                              tGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCaga                                             <  2:3612837/93‑1 (MQ=255)

                                                gTACTGAGGAAAGTCGTTGTCCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGc                                           <  1:5365275/93‑1 (MQ=255)

                                                gTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGc                                           <  1:6031341/93‑1 (MQ=255)

                                                gTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGc                                           <  2:4321831/93‑1 (MQ=255)

                                                   cTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                                        >  2:1567433/1‑93 (MQ=255)

                                                   cTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                                        <  2:1905017/93‑1 (MQ=255)

                                                     gAGCGAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCt                                      <  2:6302427/93‑1 (MQ=255)

                                                     gAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCt                                      <  2:6246466/93‑1 (MQ=255)

                                                      aGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTg                                     <  1:5647368/93‑1 (MQ=255)

                                                         aaaGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGca                                  <  1:3870430/93‑1 (MQ=255)

                                                         aaaGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGca                                  <  1:4945596/93‑1 (MQ=255)

                                                          aaGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGcac                                 >  1:4463800/1‑93 (MQ=255)

                                                               gTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACaaa                            >  1:2122153/1‑93 (MQ=255)

                                                                 tgtgCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg                          <  2:2685003/93‑1 (MQ=255)

                                                                 tgtgCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg                          <  2:124330/93‑1 (MQ=255)

                                                                  gtgCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt                         <  2:5201140/93‑1 (MQ=255)

                                                                   tgCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTg                        <  1:3704832/93‑1 (MQ=255)

                                                                   tgCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTg                        >  1:6105859/1‑93 (MQ=255)

                                                                    gCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGa                       <  1:1472480/93‑1 (MQ=255)

                                                                      cGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACg                     >  2:3767877/1‑93 (MQ=255)

                                                                      cGCAGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACg                     <  2:1395764/93‑1 (MQ=255)

                                                                       gCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                    <  2:5828255/93‑1 (MQ=255)

                                                                         aGCTTGTTAAAGAGATCGCTTCTGCTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                  <  2:1040070/93‑1 (MQ=255)

                                                                         aGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                  >  2:4375642/1‑93 (MQ=255)

                                                                         aGCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                  <  1:1565621/93‑1 (MQ=255)

                                                                          gCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGt                 >  1:2383861/1‑93 (MQ=255)

                                                                          gCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGt                 >  2:6065728/1‑93 (MQ=255)

                                                                          gCTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGt                 >  1:3414338/1‑93 (MQ=255)

                                                                           cTTGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTa                <  2:508066/93‑1 (MQ=255)

                                                                            ttGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAg               >  2:3352247/1‑93 (MQ=255)

                                                                             tGTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGt              <  2:2156170/93‑1 (MQ=255)

                                                                             tGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGt              >  1:3555719/1‑93 (MQ=255)

                                                                              gTTAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt             <  1:1090219/93‑1 (MQ=255)

                                                                               ttAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTa            <  2:4634598/93‑1 (MQ=255)

                                                                                tAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTAt           >  1:1982643/1‑93 (MQ=255)

                                                                                tAAAGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGAGACGTTGTAGGTAt           >  2:5387444/1‑93 (MQ=255)

                                                                                tAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGCAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGCTAt           >  1:4165731/1‑93 (MQ=255)

                                                                                 aaaGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATg          <  2:3047050/93‑1 (MQ=255)

                                                                                  aaGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATgg         >  2:874577/1‑93 (MQ=255)

                                                                                  aaGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATgg         >  2:3217761/1‑93 (MQ=255)

                                                                                  aaGAGATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATgg         >  1:4725739/1‑93 (MQ=255)

                                                                                   agagATCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt        >  2:5117530/1‑93 (MQ=255)

                                                                                    gagaTCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGtt       >  2:3171817/1‑93 (MQ=255)

                                                                                    gagaTCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGtt       >  1:5074205/1‑93 (MQ=255)

                                                                                      gaTCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTc     >  1:3365090/1‑93 (MQ=255)

                                                                                       aTCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCt    <  1:4415633/93‑1 (MQ=255)

                                                                                       aTCGCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCt    <  1:2475153/93‑1 (MQ=255)

                                                                                         cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg  >  1:1142144/1‑93 (MQ=255)

GCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  >  NC_000913/1754901‑1755082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  >  NC_000913/1754898‑1755082

GATGTCTCCATCCTGGCACTGACCACCAACGAAACAACCACTCATCAGTTTGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCG                                                                                              <  SRR4302547.3052190/94‑1 (MQ=60)

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCG                                                                                              <  SRR4302547.4463652/94‑1 (MQ=60)

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCG                                                                                              <  SRR4302547.3237467/94‑1 (MQ=60)

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCG                                                                                              <  SRR4302547.1531215/94‑1 (MQ=60)

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCG                                                                                              <  SRR4302547.3046423/94‑1 (MQ=60)

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCA                                                                                              <  SRR4302547.6534983/94‑1 (MQ=60)

 ATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGC                                                                                             >  SRR4302547.6307030/1‑94 (MQ=60)

   GCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTT                                                                                           >  SRR4302547.3843374/1‑94 (MQ=60)

   GCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTT                                                                                           >  SRR4302547.2202174/1‑94 (MQ=60)

   GCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTT                                                                                           <  SRR4302547.6037063/94‑1 (MQ=60)

     CACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCT                                                                                         <  SRR4302547.1745533/94‑1 (MQ=60)

     CACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCT                                                                                         >  SRR4302547.6670306/1‑94 (MQ=60)

     CACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCT                                                                                         >  SRR4302547.882542/1‑94 (MQ=60)

     CACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCT                                                                                         <  SRR4302547.665177/94‑1 (MQ=60)

        CATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACT                                                                                      >  SRR4302547.2498798/1‑94 (MQ=60)

        CATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACT                                                                                      >  SRR4302547.313402/1‑94 (MQ=60)

        CATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACT                                                                                      <  SRR4302547.6429657/94‑1 (MQ=60)

        CAGCCTGGCACTGACCACCAACGAAGAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACT                                                                                      <  SRR4302547.2969484/94‑1 (MQ=60)

         ATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTG                                                                                     <  SRR4302547.5773655/94‑1 (MQ=60)

           CCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGAT                                                                                   <  SRR4302547.5665200/94‑1 (MQ=60)

            CTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATG                                                                                  <  SRR4302547.365113/94‑1 (MQ=60)

            CTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATG                                                                                  <  SRR4302547.893905/94‑1 (MQ=60)

             TGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGT                                                                                 >  SRR4302547.2056224/1‑94 (MQ=60)

              GGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑T                                                                               >  SRR4302547.4671982/1‑94 (MQ=60)

               GCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TT                                                                              <  SRR4302547.1614520/94‑1 (MQ=60)

               GCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TT                                                                              >  SRR4302547.6479861/1‑94 (MQ=60)

                CACTGACCACCAACGAAAATGCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGACGCAGTTTGTTAAAGAGATCGCATCTACCGATGA‑TTT                                                                             <  SRR4302547.6246034/94‑1 (MQ=60)

                   TGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTA                                                                          >  SRR4302547.6249536/1‑94 (MQ=60)

                   TGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTA                                                                          <  SRR4302547.806133/94‑1 (MQ=60)

                    GACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTAC                                                                         <  SRR4302547.6169493/94‑1 (MQ=60)

                    GACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTAC                                                                         <  SRR4302547.3443507/94‑1 (MQ=60)

                      CCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCG                                                                       >  SRR4302547.6518338/1‑94 (MQ=60)

                         CCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCT                                                                    <  SRR4302547.2986072/94‑1 (MQ=60)

                         CCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCT                                                                    >  SRR4302547.1751868/1‑94 (MQ=60)

                         CCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCT                                                                    <  SRR4302547.6560722/94‑1 (MQ=60)

                           AACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGG                                                                  >  SRR4302547.4058793/1‑94 (MQ=60)

                           AACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGG                                                                  <  SRR4302547.957422/94‑1 (MQ=60)

                             CGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGT                                                                <  SRR4302547.5918036/94‑1 (MQ=60)

                             CGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGT                                                                >  SRR4302547.633489/1‑94 (MQ=60)

                             CGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGT                                                                >  SRR4302547.1821561/1‑94 (MQ=60)

                              GAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTCGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTA                                                               >  SRR4302547.3345042/1‑94 (MQ=60)

                               AAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAA                                                              >  SRR4302547.4693691/1‑94 (MQ=60)

                                 AAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAG                                                            <  SRR4302547.451261/94‑1 (MQ=60)

                                   AACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAA                                                          >  SRR4302547.4130845/1‑94 (MQ=60)

                                     CGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACT                                                        <  SRR4302547.3885566/94‑1 (MQ=60)

                                     CGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACT                                                        >  SRR4302547.4779153/1‑94 (MQ=60)

                                      GGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTG                                                       <  SRR4302547.2682071/94‑1 (MQ=60)

                                      GGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTG                                                       <  SRR4302547.2057856/94‑1 (MQ=60)

                                      GGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTG                                                       <  SRR4302547.4073721/94‑1 (MQ=60)

                                        CTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGC                                                     >  SRR4302547.6017256/1‑94 (MQ=60)

                                        CTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGC                                                     <  SRR4302547.23600/94‑1 (MQ=60)

                                         TCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCT                                                    >  SRR4302547.5526548/1‑94 (MQ=60)

                                         TCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCT                                                    <  SRR4302547.4058183/94‑1 (MQ=60)

                                         TCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCT                                                    >  SRR4302547.3373004/1‑94 (MQ=60)

                                           ATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCT                                                  <  SRR4302547.2480364/94‑1 (MQ=60)

                                              AGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGATATCGCTTCTTCTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCA                                               <  SRR4302547.3366122/94‑1 (MQ=60)

                                              AGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCA                                               >  SRR4302547.3020205/1‑94 (MQ=60)

                                              AGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCA                                               <  SRR4302547.4600874/94‑1 (MQ=60)

                                              AGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCA                                               >  SRR4302547.5757199/1‑94 (MQ=60)

                                              AGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCA                                               >  SRR4302547.4347933/1‑94 (MQ=60)

                                               GTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAG                                              <  SRR4302547.3047171/94‑1 (MQ=60)

                                                TTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGA                                             >  SRR4302547.4060790/1‑94 (MQ=60)

                                                TTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGA                                             <  SRR4302547.768610/94‑1 (MQ=60)

                                                TTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGA                                             <  SRR4302547.2838010/94‑1 (MQ=60)

                                                   GTACTGAGGAAAGTCGTTGTCCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCG                                          <  SRR4302547.5475969/94‑1 (MQ=60)

                                                   GTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCG                                          <  SRR4302547.6156506/94‑1 (MQ=60)

                                                       TGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCT                                      <  SRR4302547.4399771/94‑1 (MQ=60)

                                                       TGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCT                                      <  SRR4302547.4680019/94‑1 (MQ=60)

                                                       TGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCT                                      >  SRR4302547.4911054/1‑94 (MQ=60)

                                                        GAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTG                                     <  SRR4302547.5926922/94‑1 (MQ=60)

                                                        GAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTG                                     <  SRR4302547.1496800/94‑1 (MQ=60)

                                                         AGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGG                                    <  SRR4302547.5763932/94‑1 (MQ=60)

                                                         AGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGG                                    >  SRR4302547.3657837/1‑94 (MQ=60)

                                                          GCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGC                                   <  SRR4302547.3555175/94‑1 (MQ=60)

                                                           CAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCA                                  >  SRR4302547.5303450/1‑94 (MQ=60)

                                                            AAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCAC                                 <  SRR4302547.3949216/94‑1 (MQ=60)

                                                            AAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCAC                                 >  SRR4302547.4556039/1‑94 (MQ=60)

                                                            AAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCAC                                 <  SRR4302547.5048022/94‑1 (MQ=60)

                                                                GCGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAA                             <  SRR4302547.4223022/94‑1 (MQ=60)

                                                                 CGTTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAA                            >  SRR4302547.2164454/1‑94 (MQ=60)

                                                                   TTGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGG                          >  SRR4302547.2298787/1‑94 (MQ=60)

                                                                    TGTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGG                         >  SRR4302547.2127468/1‑94 (MQ=60)

                                                                     GTGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTG                        >  SRR4302547.6232513/1‑94 (MQ=60)

                                                                     GTGCCGCAGCTGGTTAAAGAGGTCGCTTTTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTG                        <  SRR4302547.2020023/94‑1 (MQ=60)

                                                                      TGCCGCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGA                       <  SRR4302547.3780457/94‑1 (MQ=60)

                                                                      TGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGA                       >  SRR4302547.100120/1‑94 (MQ=60)

                                                                       GCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGAC                      <  SRR4302547.1501890/94‑1 (MQ=60)

                                                                         CGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTTTTACCGGCTGGGTAAAGACCTGGCTCTGCGGACCGGCCTGGCACACAAAGGggccg                     >  SRR4302547.4592697/1‑89 (MQ=60)

                                                                          GCAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTT                   <  SRR4302547.5089405/94‑1 (MQ=60)

                                                                           CAGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTG                  >  SRR4302547.2540182/1‑94 (MQ=60)

                                                                            AGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGT                 >  SRR4302547.3483863/1‑94 (MQ=60)

                                                                            AGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGT                 >  SRR4302547.2432158/1‑94 (MQ=60)

                                                                            AGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGT                 >  SRR4302547.1114347/1‑94 (MQ=60)

                                                                            AGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGT                 >  SRR4302547.411813/1‑94 (MQ=60)

                                                                            AGCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGT                 <  SRR4302547.1596802/94‑1 (MQ=60)

                                                                             GCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTA                <  SRR4302547.2444419/94‑1 (MQ=60)

                                                                             GCTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTA                <  SRR4302547.6234021/94‑1 (MQ=60)

                                                                              CTTGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAG               >  SRR4302547.2994810/1‑94 (MQ=60)

                                                                               TTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGT              >  SRR4302547.3628280/1‑94 (MQ=60)

                                                                                TGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTT             <  SRR4302547.1575246/94‑1 (MQ=60)

                                                                                TGTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTT             <  SRR4302547.6330570/94‑1 (MQ=60)

                                                                                 GTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTA            <  SRR4302547.1112063/94‑1 (MQ=60)

                                                                                 GTTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTA            >  SRR4302547.3897774/1‑94 (MQ=60)

                                                                                  TTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTAT           >  SRR4302547.2022011/1‑94 (MQ=60)

                                                                                  TTAAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGGTGTAGTTAT           >  SRR4302547.240374/1‑94 (MQ=60)

                                                                                  TTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGCAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGCTAT           >  SRR4302547.4250577/1‑94 (MQ=60)

                                                                                    AAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGG         >  SRR4302547.4823645/1‑94 (MQ=60)

                                                                                    AAAGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGCCACACAAAGGTGAAGTTGTAGTTATGG         <  SRR4302547.3508392/94‑1 (MQ=60)

                                                                                      AGAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTT       >  SRR4302547.5178891/1‑94 (MQ=60)

                                                                                       GAGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTT      <  SRR4302547.207361/94‑1 (MQ=60)

                                                                                       GAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTT      >  SRR4302547.543097/1‑94 (MQ=60)

                                                                                        AGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTC     >  SRR4302547.2200489/1‑94 (MQ=60)

                                                                                        AGATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTC     >  SRR4302547.3433427/1‑94 (MQ=60)

                                                                                          ATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTG   <  SRR4302547.2525201/94‑1 (MQ=60)

                                                                                          ATCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTG   <  SRR4302547.4506725/94‑1 (MQ=60)

                                                                                           TCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  <  SRR4302547.2210329/94‑1 (MQ=60)

                                                                                           TCGCTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  <  SRR4302547.536096/94‑1 (MQ=60)

                                                                                           TCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  >  SRR4302547.1165217/1‑94 (MQ=60)

GATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGA‑TTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGG  >  NC_000913/1754898‑1755082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`