Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,971,922 T→C D476G (GAC→GGC)  cheA ← CheA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,971,9220TC90.4% 611.7 / 42.0 208D476G (GAC→GGC) cheACheA
Reads supporting (aligned to +/- strand):  ref base T (12/8);  new base C (94/94);  total (106/102)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 213 total aligned reads displayed.

GGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGC  >  NC_000913/1971832‑1972012
                                                                                          |                                                                                          
ggCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGccc                                                                                          <  1:5923229/93‑1 (MQ=255)
  cAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCAt                                                                                        <  2:1476274/93‑1 (MQ=255)
  cAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCAt                                                                                        >  2:2954541/1‑93 (MQ=255)
  cAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCAt                                                                                        <  1:577493/93‑1 (MQ=255)
   aGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATg                                                                                       >  1:6470316/1‑93 (MQ=255)
   aGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATg                                                                                       <  2:3457866/93‑1 (MQ=255)
   aGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATg                                                                                       <  2:3984222/93‑1 (MQ=255)
   aGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATg                                                                                       <  1:6068750/93‑1 (MQ=255)
     tAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGcc                                                                                     <  2:50579/93‑1 (MQ=255)
     tAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGcc                                                                                     >  1:5746438/1‑93 (MQ=255)
      aaaaTGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCg                                                                                    <  2:3576625/93‑1 (MQ=255)
       aaaTGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGa                                                                                   >  1:2530906/1‑93 (MQ=255)
       aaaTGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGa                                                                                   <  2:563730/93‑1 (MQ=255)
             ggATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGAcgccgc                                                                             <  1:6116600/93‑1 (MQ=255)
              gATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCcgcg                                                                            >  2:1574166/1‑93 (MQ=255)
                tCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGcc                                                                          <  2:2891721/93‑1 (MQ=255)
                  gTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCg                                                                        >  2:2873204/1‑93 (MQ=255)
                    aGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGga                                                                      <  1:4860492/93‑1 (MQ=255)
                     gTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGgag                                                                     >  2:6314013/1‑93 (MQ=255)
                     gTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGgag                                                                     >  2:5785887/1‑93 (MQ=255)
                        ccAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACg                                                                  >  2:6119986/1‑93 (MQ=255)
                         cAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGt                                                                 >  2:3621144/1‑93 (MQ=255)
                          aGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTc                                                                <  2:3062222/93‑1 (MQ=255)
                           gTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCg                                                               <  2:292603/93‑1 (MQ=255)
                            tACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCgg                                                              >  2:3346614/1‑93 (MQ=255)
                            tACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCgg                                                              <  2:6465477/93‑1 (MQ=255)
                             tcccTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGt                                                             <  1:3168773/92‑1 (MQ=255)
                             aCCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGt                                                             <  1:1452279/93‑1 (MQ=255)
                              cccTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTg                                                            >  2:5571138/1‑93 (MQ=255)
                               ccTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGa                                                           >  1:2138412/1‑93 (MQ=255)
                                cTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGAc                                                          >  1:5722461/1‑93 (MQ=255)
                                cTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCACCCGGAGACGTCGGTGAc                                                          >  1:1112244/1‑93 (MQ=255)
                                 tGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGAcc                                                         <  1:6331263/93‑1 (MQ=255)
                                 cgCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGAcc                                                         <  2:4196199/92‑1 (MQ=255)
                                  gCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCt                                                        >  1:1553662/1‑93 (MQ=255)
                                   cTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTg                                                       >  2:6588332/1‑93 (MQ=255)
                                   cTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTg                                                       >  2:1167688/1‑93 (MQ=255)
                                      cGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGctc                                                    <  1:2792161/93‑1 (MQ=255)
                                      cGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGctc                                                    <  1:679304/93‑1 (MQ=255)
                                       gACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGctct                                                   >  1:11540/1‑93 (MQ=255)
                                        aCTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTg                                                  >  2:6102772/1‑93 (MQ=255)
                                        aCTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTg                                                  >  1:1925454/1‑93 (MQ=255)
                                        aCTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTg                                                  <  1:3040726/93‑1 (MQ=255)
                                         cTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGc                                                 <  2:1883985/93‑1 (MQ=255)
                                         cTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGc                                                 <  2:810211/93‑1 (MQ=255)
                                            gATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGt                                              >  2:1339582/1‑93 (MQ=255)
                                            gATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGt                                              <  1:1767751/93‑1 (MQ=255)
                                             aTTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTg                                             <  1:881590/93‑1 (MQ=255)
                                             aTTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTg                                             <  1:6190379/93‑1 (MQ=255)
                                                tCGACATGAGCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGgag                                          >  2:874054/1‑93 (MQ=255)
                                                tCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGgag                                          >  1:1340363/1‑93 (MQ=255)
                                                 cGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGgaga                                         <  2:4846252/93‑1 (MQ=255)
                                                    cATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGGGAAGc                                      >  2:2985836/1‑93 (MQ=255)
                                                    cATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGc                                      >  2:4343848/1‑93 (MQ=255)
                                                    cATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGc                                      <  1:3629399/93‑1 (MQ=255)
                                                     aTGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGcc                                     >  2:1264452/1‑93 (MQ=255)
                                                     aTGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGcc                                     <  2:4266960/93‑1 (MQ=255)
                                                      tGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCa                                    <  2:266723/93‑1 (MQ=255)
                                                      tGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCa                                    <  1:3310009/93‑1 (MQ=255)
                                                      tGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCa                                    >  2:5861750/1‑93 (MQ=255)
                                                        aCCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAgg                                  <  1:4121896/93‑1 (MQ=255)
                                                         ccGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGt                                 <  1:51706/93‑1 (MQ=255)
                                                          cGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTg                                >  1:4621759/1‑93 (MQ=255)
                                                           gCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGc                               <  1:1639416/93‑1 (MQ=255)
                                                           ccccATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGc                               <  2:6246638/92‑1 (MQ=255)
                                                             ccATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCaa                             >  2:5707965/1‑93 (MQ=255)
                                                             ccATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCaa                             >  1:3022617/1‑93 (MQ=255)
                                                               aTCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAat                           >  1:3563247/1‑93 (MQ=255)
                                                                 cttctGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAatat                         <  2:1471168/93‑1 (MQ=255)
                                                                   tctGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCa                       <  2:3661960/93‑1 (MQ=255)
                                                                     tGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAga                     >  1:42087/1‑92 (MQ=255)
                                                                      ggATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCa                    <  2:2211179/93‑1 (MQ=255)
                                                                       gATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCAt                   <  2:1722876/93‑1 (MQ=255)
                                                                       gATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCAt                   <  1:5026173/93‑1 (MQ=255)
                                                                         tatTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATcg                 <  1:2619207/93‑1 (MQ=255)
                                                                         tatTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATcg                 <  1:3382453/93‑1 (MQ=255)
                                                                          atTACGTTTAACGACGCCCATGCCTACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATcgc                <  1:4366120/93‑1 (MQ=255)
                                                                           ttACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATcgcg               >  2:2754523/1‑93 (MQ=255)
                                                                            tACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGa              >  1:3102797/1‑93 (MQ=255)
                                                                            tACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGa              <  2:665074/93‑1 (MQ=255)
                                                                             aCGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGAc             <  1:2366118/93‑1 (MQ=255)
                                                                             aCGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGAc             >  1:5124259/1‑93 (MQ=255)
                                                                              cGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACt            <  1:4177152/93‑1 (MQ=255)
                                                                              cGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACt            >  1:4737211/1‑93 (MQ=255)
                                                                               gTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCCGGTGCAAATATCAGCATCGCGACtt           <  1:2308294/93‑1 (MQ=255)
                                                                               gTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACtt           <  2:5172528/93‑1 (MQ=255)
                                                                               gTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACtt           <  2:2369369/93‑1 (MQ=255)
                                                                                tttAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtc          >  2:3566216/1‑93 (MQ=255)
                                                                                 ttAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcg         >  1:2717214/1‑93 (MQ=255)
                                                                                  tAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgt        <  1:4010477/93‑1 (MQ=255)
                                                                                     cgacgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgt     <  1:4511125/93‑1 (MQ=255)
                                                                                     cgacgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgt     >  2:360185/1‑93 (MQ=255)
                                                                                      gacgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgtc    <  1:997594/93‑1 (MQ=255)
                                                                                      gacgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgtc    <  1:1646534/93‑1 (MQ=255)
                                                                                       acgTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgtcg   >  1:2975113/1‑93 (MQ=255)
                                                                                       acgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgtcg   >  2:3561278/1‑93 (MQ=255)
                                                                                       acgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgtcg   >  1:3737268/1‑93 (MQ=255)
                                                                                       acgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtcgtcgtcg   >  2:947232/1‑93 (MQ=255)
                                                                                       acgCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGAGCAAATATCAGCATCGCGACTtcgtcgtcg   >  2:4711341/1‑93 (MQ=255)
                                                                                        cgTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGc  <  1:3933256/93‑1 (MQ=255)
                                                                                          |                                                                                          
GGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGC  >  NC_000913/1971832‑1972012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCA  >  NC_000913/1971829‑1972015
                                                                                             |                                                                                             
AGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGC                                                                                               >  SRR4302547.4328384/1‑94 (MQ=60)
 GCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCC                                                                                              <  SRR4302547.3292939/94‑1 (MQ=60)
   GGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCA                                                                                            <  SRR4302547.6045992/94‑1 (MQ=60)
    GCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCAT                                                                                           <  SRR4302547.6174107/94‑1 (MQ=60)
     CAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATG                                                                                          >  SRR4302547.6605053/1‑94 (MQ=60)
     CAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATG                                                                                          <  SRR4302547.587845/94‑1 (MQ=60)
      AGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGC                                                                                         <  SRR4302547.6194622/94‑1 (MQ=60)
       GTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCC                                                                                        <  SRR4302547.4278394/94‑1 (MQ=60)
       GTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCC                                                                                        >  SRR4302547.5865211/1‑94 (MQ=60)
        TAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCG                                                                                       <  SRR4302547.2388153/94‑1 (MQ=60)
        TAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCG                                                                                       >  SRR4302547.6533329/1‑94 (MQ=60)
         AAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGA                                                                                      >  SRR4302547.2582060/1‑94 (MQ=60)
          AAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGAC                                                                                     <  SRR4302547.3642005/94‑1 (MQ=60)
               CGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGC                                                                                >  SRR4302547.5241565/1‑94 (MQ=60)
                GGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCG                                                                               <  SRR4302547.6243479/94‑1 (MQ=60)
                     GTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGG                                                                          >  SRR4302547.3252310/1‑94 (MQ=60)
                     GTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCAGACGCCGCGCCCGG                                                                          <  SRR4302547.2287677/94‑1 (MQ=60)
                      TAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGA                                                                         >  SRR4302547.4066885/1‑94 (MQ=60)
                      TAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGA                                                                         >  SRR4302547.1582939/1‑94 (MQ=60)
                       AGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAG                                                                        <  SRR4302547.4961218/94‑1 (MQ=60)
                             AGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCG                                                                  >  SRR4302547.2165940/1‑94 (MQ=60)
                             AGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCG                                                                  <  SRR4302547.3084596/94‑1 (MQ=60)
                             AGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCG                                                                  <  SRR4302547.2731141/94‑1 (MQ=60)
                             AGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCG                                                                  <  SRR4302547.4497227/94‑1 (MQ=60)
                               TACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGT                                                                >  SRR4302547.2942629/1‑94 (MQ=60)
                                TCCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTG                                                               <  SRR4302547.3231827/94‑1 (MQ=60)
                                ACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTG                                                               <  SRR4302547.1481318/94‑1 (MQ=60)
                                 CCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGA                                                              >  SRR4302547.2181048/1‑94 (MQ=60)
                                 CCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGA                                                              <  SRR4302547.3155822/94‑1 (MQ=60)
                                 CCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGA                                                              >  SRR4302547.4004733/1‑94 (MQ=60)
                                  CCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGAC                                                             >  SRR4302547.6370542/1‑94 (MQ=60)
                                  CCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGAC                                                             >  SRR4302547.5840678/1‑94 (MQ=60)
                                  CCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCACCCGGAGACGTCGGTGAC                                                             >  SRR4302547.1134644/1‑94 (MQ=60)
                                   CTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACC                                                            >  SRR4302547.1415440/1‑94 (MQ=60)
                                   CTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACC                                                            >  SRR4302547.5790088/1‑94 (MQ=60)
                                    TGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCT                                                           <  SRR4302547.6463053/94‑1 (MQ=60)
                                    TGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCT                                                           >  SRR4302547.1584580/1‑94 (MQ=60)
                                     GCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTG                                                          <  SRR4302547.29392/94‑1 (MQ=60)
                                       TTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCT                                                        <  SRR4302547.3220469/94‑1 (MQ=60)
                                         CGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCT                                                      <  SRR4302547.2848074/94‑1 (MQ=60)
                                         CGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCT                                                      <  SRR4302547.691826/94‑1 (MQ=60)
                                         CGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCT                                                      >  SRR4302547.11752/1‑94 (MQ=60)
                                         CGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCT                                                      <  SRR4302547.1143640/94‑1 (MQ=60)
                                          GACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTG                                                     >  SRR4302547.4424523/1‑94 (MQ=60)
                                          GACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTG                                                     <  SRR4302547.2460509/94‑1 (MQ=60)
                                          GACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTG                                                     >  SRR4302547.1963598/1‑94 (MQ=60)
                                           ACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGC                                                    >  SRR4302547.164190/1‑94 (MQ=60)
                                           ACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGC                                                    <  SRR4302547.3101247/94‑1 (MQ=60)
                                             TGGTTTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCG                                                  <  SRR4302547.1848802/94‑1 (MQ=60)
                                              GGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGT                                                 >  SRR4302547.657987/1‑94 (MQ=60)
                                               GATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTG                                                <  SRR4302547.1802811/94‑1 (MQ=60)
                                               GATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCCCCCGGAGACGTCGGTGACCTGCTCTGCCGTG                                                >  SRR4302547.5640326/1‑94 (MQ=60)
                                                ATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGG                                               <  SRR4302547.6318885/94‑1 (MQ=60)
                                                ATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGG                                               <  SRR4302547.898533/94‑1 (MQ=60)
                                                ATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGG                                               >  SRR4302547.3662947/1‑94 (MQ=60)
                                                  TTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAG                                             >  SRR4302547.1367298/1‑94 (MQ=60)
                                                       CATGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCC                                        <  SRR4302547.3703436/94‑1 (MQ=60)
                                                         TGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAG                                      <  SRR4302547.3376753/94‑1 (MQ=60)
                                                         TGACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAG                                      <  SRR4302547.429372/94‑1 (MQ=60)
                                                           ACCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGT                                    <  SRR4302547.4205658/94‑1 (MQ=60)
                                                            CCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTG                                   <  SRR4302547.52677/94‑1 (MQ=60)
                                                            CCGCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTG                                   >  SRR4302547.4717464/1‑94 (MQ=60)
                                                              GCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCA                                 <  SRR4302547.1671822/94‑1 (MQ=60)
                                                              GCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCA                                 <  SRR4302547.6201297/94‑1 (MQ=60)
                                                              GCCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCA                                 >  SRR4302547.125329/1‑94 (MQ=60)
                                                               CCCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAA                                >  SRR4302547.3082723/1‑94 (MQ=60)
                                                                CCATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAA                               >  SRR4302547.2646321/1‑94 (MQ=60)
                                                                 CATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAAT                              >  SRR4302547.3635947/1‑94 (MQ=60)
                                                                  ATCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATA                             >  SRR4302547.5108683/1‑94 (MQ=60)
                                                                   TCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATAT                            >  SRR4302547.3737128/1‑94 (MQ=60)
                                                                   TCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATAT                            <  SRR4302547.4212976/94‑1 (MQ=60)
                                                                   TCTTCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATAT                            <  SRR4302547.4688802/94‑1 (MQ=60)
                                                                      TCTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAG                         <  SRR4302547.4870255/94‑1 (MQ=60)
                                                                       CTGGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGA                        >  SRR4302547.42861/1‑94 (MQ=60)
                                                                        TGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCA                       >  SRR4302547.6770423/1‑94 (MQ=60)
                                                                         GGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCAT                      >  SRR4302547.1676693/1‑94 (MQ=60)
                                                                         GGATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCAT                      <  SRR4302547.1752126/94‑1 (MQ=60)
                                                                          GATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATC                     >  SRR4302547.477182/1‑94 (MQ=60)
                                                                          GATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATC                     <  SRR4302547.5130023/94‑1 (MQ=60)
                                                                           ATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCG                    >  SRR4302547.5872686/1‑94 (MQ=60)
                                                                           ATATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCG                    <  SRR4302547.6010555/94‑1 (MQ=60)
                                                                            TATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGC                   <  SRR4302547.3399413/94‑1 (MQ=60)
                                                                            TATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGC                   <  SRR4302547.2671983/94‑1 (MQ=60)
                                                                            TATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGC                   <  SRR4302547.3451210/94‑1 (MQ=60)
                                                                             ATTACGTTTAACGACGCCCATGCCTACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCG                  <  SRR4302547.4455787/94‑1 (MQ=60)
                                                                             ATTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCG                  >  SRR4302547.6272654/1‑94 (MQ=60)
                                                                              TTACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGA                 >  SRR4302547.3164290/1‑94 (MQ=60)
                                                                               TACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGAC                >  SRR4302547.5229848/1‑94 (MQ=60)
                                                                                ACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACT               <  SRR4302547.2414081/94‑1 (MQ=60)
                                                                                ACGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACT               >  SRR4302547.4835392/1‑94 (MQ=60)
                                                                                 CGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTT              <  SRR4302547.4262257/94‑1 (MQ=60)
                                                                                 CGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTT              >  SRR4302547.2781369/1‑94 (MQ=60)
                                                                                 CGTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTT              <  SRR4302547.1866569/94‑1 (MQ=60)
                                                                                  GTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCCGGTGCAAATATCAGCATCGCGACTTC             <  SRR4302547.2355092/94‑1 (MQ=60)
                                                                                  GTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTC             <  SRR4302547.5175214/94‑1 (MQ=60)
                                                                                  GTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTC             <  SRR4302547.4190351/94‑1 (MQ=60)
                                                                                  GTTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGCCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATAGCGACTTC             >  SRR4302547.353077/1‑94 (MQ=60)
                                                                                   TTTAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCG            >  SRR4302547.2771762/1‑94 (MQ=60)
                                                                                   TTCAACGACGCCCGTGCCGACGCCGTCCCCAGAGTCTTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATAAGCATCGCGCCTTCG            <  SRR4302547.4273346/94‑1 (MQ=48)
                                                                                     TAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTC          <  SRR4302547.863483/94‑1 (MQ=60)
                                                                                     TAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTC          <  SRR4302547.1110632/94‑1 (MQ=60)
                                                                                     TAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTC          <  SRR4302547.4091993/94‑1 (MQ=60)
                                                                                     TAACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTC          <  SRR4302547.3046254/94‑1 (MQ=60)
                                                                                      AACGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCG         >  SRR4302547.5846025/1‑94 (MQ=60)
                                                                                        CGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTC       <  SRR4302547.4604329/94‑1 (MQ=60)
                                                                                        CGACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTC       <  SRR4302547.959072/94‑1 (MQ=60)
                                                                                         GACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCG      >  SRR4302547.3034315/1‑94 (MQ=60)
                                                                                         GACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCG      >  SRR4302547.3813574/1‑94 (MQ=60)
                                                                                         GACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCG      <  SRR4302547.1679058/94‑1 (MQ=60)
                                                                                         GACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCG      <  SRR4302547.1017044/94‑1 (MQ=60)
                                                                                          ACGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGC     >  SRR4302547.2164801/1‑94 (MQ=60)
                                                                                           CGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCT    <  SRR4302547.4013256/94‑1 (MQ=60)
                                                                                           CGCCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCT    >  SRR4302547.2398920/1‑94 (MQ=60)
                                                                                             CCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCA  >  SRR4302547.3466350/1‑94 (MQ=60)
                                                                                             CCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCA  >  SRR4302547.2410877/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCA  >  NC_000913/1971829‑1972015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑