Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,422,395 A→G S45S (AGT→AGC)  hisP ← ATP‑binding component of histidine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,422,3950AG87.2% 570.2 / 64.0 219S45S (AGT→AGC) hisPATP‑binding component of histidine transport
Reads supporting (aligned to +/- strand):  ref base A (13/15);  new base G (81/110);  total (94/125)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

Only 100 of 226 total aligned reads displayed.

TCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTA  >  NC_000913/2422303‑2422487
                                                                                            |                                                                                            
tCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTa                                                                                              >  2:3437953/1‑93 (MQ=255)
 cTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTAc                                                                                             <  1:614759/93‑1 (MQ=255)
  tttGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCt                                                                                            <  1:2804300/93‑1 (MQ=255)
   ttGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACtt                                                                                           <  1:5471846/93‑1 (MQ=255)
    tGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCttt                                                                                          <  2:814836/93‑1 (MQ=255)
    tGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCttt                                                                                          <  1:3134243/93‑1 (MQ=255)
    tGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACttt                                                                                          <  2:5395936/93‑1 (MQ=255)
     gTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCtttt                                                                                         >  2:4419086/1‑93 (MQ=255)
     gTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACtttt                                                                                         >  1:177134/1‑93 (MQ=255)
      tCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTc                                                                                        <  2:2520375/93‑1 (MQ=255)
        gcgcACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTccc                                                                                      >  1:3310146/1‑93 (MQ=255)
         cgcACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTcccc                                                                                     <  1:88045/93‑1 (MQ=255)
          gcACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCg                                                                                    >  2:6599564/1‑93 (MQ=255)
            aCCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGAt                                                                                  >  1:343533/1‑93 (MQ=255)
            aCCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGAt                                                                                  >  2:83751/1‑93 (MQ=255)
             ccAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATc                                                                                 >  2:6254959/1‑93 (MQ=255)
              cAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATcc                                                                                <  1:823182/93‑1 (MQ=255)
               aGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATccc                                                                               >  2:1022321/1‑93 (MQ=255)
               aGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATccc                                                                               <  2:2037063/93‑1 (MQ=255)
                gATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCcg                                                                              <  1:4255908/93‑1 (MQ=255)
                gATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTATTCCCCGATCCcg                                                                              >  1:4226404/1‑93 (MQ=255)
                 aTTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCcga                                                                             <  1:4758255/93‑1 (MQ=255)
                  ttGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCcgac                                                                            <  2:3983964/93‑1 (MQ=255)
                    gATCGTCTTGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCcgacga                                                                          <  2:428486/93‑1 (MQ=255)
                    gATCGTCTGGCCATTGACTACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGAGCTTTTCCCCGATCCcgacga                                                                          >  2:1753079/1‑93 (MQ=255)
                     aTCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGAt                                                                         <  1:5556440/93‑1 (MQ=255)
                      tCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATc                                                                        >  1:2488484/1‑93 (MQ=255)
                      tCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATc                                                                        <  1:4715153/93‑1 (MQ=255)
                       cGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATcc                                                                       <  1:1574706/93‑1 (MQ=255)
                         tCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCga                                                                     >  2:1647289/1‑93 (MQ=255)
                           tGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCgatg                                                                   >  2:3223562/1‑93 (MQ=255)
                           tGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCgatg                                                                   <  2:292254/93‑1 (MQ=255)
                            ggCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCgatga                                                                  <  2:5107479/93‑1 (MQ=255)
                            ggCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCgatga                                                                  >  1:1725650/1‑93 (MQ=255)
                            ggCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCgatga                                                                  >  1:4018743/1‑93 (MQ=255)
                             gCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCgatgat                                                                 <  2:351972/93‑1 (MQ=255)
                               cATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGc                                                               >  1:2713390/1‑93 (MQ=255)
                                aTTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCt                                                              <  2:3625482/93‑1 (MQ=255)
                                aTTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCt                                                              <  1:2210381/93‑1 (MQ=255)
                                aTTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCt                                                              >  2:4355123/1‑93 (MQ=255)
                                 ttGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCtt                                                             <  1:3884520/93‑1 (MQ=255)
                                  tGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCtta                                                            <  1:2988211/93‑1 (MQ=255)
                                   gACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCttat                                                           >  2:2790321/1‑93 (MQ=255)
                                    aCCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCttatt                                                          >  1:2952153/1‑93 (MQ=255)
                                     ccACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCttatta                                                         <  1:2736016/93‑1 (MQ=255)
                                       aCGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACa                                                       >  2:2176968/1‑93 (MQ=255)
                                       aCGATCCACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACa                                                       >  1:4559046/1‑93 (MQ=255)
                                         gATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACAtc                                                     >  2:2704237/1‑93 (MQ=255)
                                         gATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACAtc                                                     <  1:1250643/93‑1 (MQ=255)
                                          aTCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACAtct                                                    <  1:1149619/93‑1 (MQ=255)
                                          aTCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACAtct                                                    <  1:6306114/93‑1 (MQ=255)
                                             gACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCg                                                 >  2:4082639/1‑93 (MQ=255)
                                              aCCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCgg                                                >  1:920927/1‑93 (MQ=255)
                                               cccTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGc                                               >  1:714811/1‑93 (MQ=255)
                                               cccTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGGGCTTTTCTCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGc                                               >  1:2340146/1‑93 (MQ=255)
                                                 cTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATCACATCTCCGGCAt                                             <  1:3179450/93‑1 (MQ=255)
                                                    cACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCg                                          <  1:1928595/93‑1 (MQ=255)
                                                     aCTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGc                                         <  2:755683/93‑1 (MQ=255)
                                                     aCTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGc                                         >  1:1060993/1‑93 (MQ=255)
                                                       tCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCtt                                       >  1:750023/1‑93 (MQ=255)
                                                       tCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCtt                                       >  2:640315/1‑93 (MQ=255)
                                                       tCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCtt                                       <  1:5741830/93‑1 (MQ=255)
                                                       tCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCtt                                       >  2:4253431/1‑93 (MQ=255)
                                                       tCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCtt                                       <  2:4225116/93‑1 (MQ=255)
                                                          gTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCa                                    >  1:3700514/1‑93 (MQ=255)
                                                           tttttCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAg                                   >  1:1118720/1‑93 (MQ=255)
                                                           tttttCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAg                                   <  1:2593343/93‑1 (MQ=255)
                                                           tttttCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAg                                   <  1:99184/93‑1 (MQ=255)
                                                            ttttCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGt                                  >  2:1947521/1‑93 (MQ=255)
                                                              ttCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGa                                >  1:1410135/1‑93 (MQ=255)
                                                              ttCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGAGCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGa                                <  2:273822/93‑1 (MQ=255)
                                                                 gAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATAc                             >  2:1922472/1‑93 (MQ=255)
                                                                  aGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATAcc                            >  1:1000664/1‑93 (MQ=255)
                                                                  aGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATAcc                            >  1:355372/1‑93 (MQ=255)
                                                                     aaGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATTATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCt                         <  2:5312064/93‑1 (MQ=255)
                                                                     aaGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCt                         >  2:5652465/1‑93 (MQ=255)
                                                                      aGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCtt                        <  1:4577133/93‑1 (MQ=255)
                                                                       gTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCttt                       >  1:5975909/1‑93 (MQ=255)
                                                                        ttAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTc                      <  1:6226056/93‑1 (MQ=255)
                                                                        ttAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTc                      <  1:5161174/93‑1 (MQ=255)
                                                                        ttAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTc                      <  1:266714/93‑1 (MQ=255)
                                                                          aaTGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAg                    >  1:5144974/1‑93 (MQ=255)
                                                                          aaTGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAg                    >  1:5498285/1‑93 (MQ=255)
                                                                          aaTGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAg                    <  1:3651681/93‑1 (MQ=255)
                                                                            aggaGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCa                  <  2:1382532/90‑1 (MQ=255)
                                                                                  gaaGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCAt            <  1:1363058/91‑1 (MQ=255)
                                                                                   cAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATg           <  1:644554/93‑1 (MQ=255)
                                                                                    aGAAAGTTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGt          <  1:5629346/93‑1 (MQ=255)
                                                                                    aGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGt          >  2:4795684/1‑93 (MQ=255)
                                                                                    aGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGt          <  1:3756770/93‑1 (MQ=255)
                                                                                    aGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGt          <  1:5215989/93‑1 (MQ=255)
                                                                                     gAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGtt         >  2:1232205/1‑93 (MQ=255)
                                                                                      aaaGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTc        <  1:5707268/93‑1 (MQ=255)
                                                                                      aaaGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTc        >  2:5144799/1‑93 (MQ=255)
                                                                                      aaaGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTc        >  2:3651557/1‑93 (MQ=255)
                                                                                       aaGGTGCTTTTCGCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCg       >  1:5310027/1‑93 (MQ=255)
                                                                                       aaGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCg       >  1:1825798/1‑93 (MQ=255)
                                                                                        aGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGc      <  1:5167036/93‑1 (MQ=255)
                                                                                        aGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGc      <  2:3131611/93‑1 (MQ=255)
                                                                                            aCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTa  <  1:4363531/93‑1 (MQ=255)
                                                                                            |                                                                                            
TCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTA  >  NC_000913/2422303‑2422487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAG  >  NC_000913/2422302‑2422488
                                                                                             |                                                                                             
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTG                                                                                               >  SRR4302547.3987528/1‑94 (MQ=60)
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTG                                                                                               <  SRR4302547.3958112/94‑1 (MQ=60)
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTG                                                                                               <  SRR4302547.697594/94‑1 (MQ=60)
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTG                                                                                               >  SRR4302547.2762642/1‑94 (MQ=60)
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTG                                                                                               <  SRR4302547.1676996/94‑1 (MQ=60)
 TCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGC                                                                                              >  SRR4302547.3324595/1‑94 (MQ=60)
  cttcgtgcCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCCCTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCT                                                                                             <  SRR4302547.5936038/86‑1 (MQ=60)
  CTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCT                                                                                             >  SRR4302547.2892171/1‑94 (MQ=60)
  CTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACT                                                                                             <  SRR4302547.626149/94‑1 (MQ=60)
   TTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTT                                                                                            <  SRR4302547.2860440/94‑1 (MQ=60)
    TTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTT                                                                                           <  SRR4302547.1800052/94‑1 (MQ=60)
    TTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTT                                                                                           >  SRR4302547.3022762/1‑94 (MQ=60)
    TTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTT                                                                                           <  SRR4302547.5584882/94‑1 (MQ=60)
     TGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTT                                                                                          <  SRR4302547.6307835/94‑1 (MQ=60)
     TGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTT                                                                                          <  SRR4302547.917631/94‑1 (MQ=60)
     TGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTT                                                                                          <  SRR4302547.3196471/94‑1 (MQ=60)
     TGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTT                                                                                          >  SRR4302547.180417/1‑94 (MQ=60)
      GTCGCGCACCAGTTTGAGCGTCTGGCCATTGACCGCGATCGTCCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTC                                                                                         <  SRR4302547.4337065/94‑1 (MQ=60)
      GTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTC                                                                                         <  SRR4302547.5849798/94‑1 (MQ=60)
        CGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCC                                                                                       <  SRR4302547.5594400/94‑1 (MQ=60)
        CGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCC                                                                                       >  SRR4302547.3376894/1‑94 (MQ=60)
         caGCACCAGATTGATCGTCTGGCCATTGACCACGATCGCCCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCC                                                                                      <  SRR4302547.376641/92‑1 (MQ=60)
         GCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCC                                                                                      <  SRR4302547.5240027/94‑1 (MQ=60)
         GCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCC                                                                                      >  SRR4302547.2634811/1‑94 (MQ=60)
          CGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCG                                                                                     <  SRR4302547.89684/94‑1 (MQ=60)
            CACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGAT                                                                                   >  SRR4302547.349914/1‑94 (MQ=60)
             ACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATC                                                                                  >  SRR4302547.5713981/1‑94 (MQ=60)
               CAGATTGCTCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCC                                                                                <  SRR4302547.4642754/94‑1 (MQ=60)
               CAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCC                                                                                <  SRR4302547.838890/94‑1 (MQ=60)
                AGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCG                                                                               >  SRR4302547.5944296/1‑94 (MQ=60)
                AGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCG                                                                               >  SRR4302547.4704742/1‑94 (MQ=60)
                AGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTATTCCCCGATCCCG                                                                               >  SRR4302547.4312771/1‑94 (MQ=60)
                 GATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGA                                                                              >  SRR4302547.5413518/1‑94 (MQ=60)
                 GATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGA                                                                              <  SRR4302547.4342994/94‑1 (MQ=60)
                  ATTGATCGTCTGGCCATTGACCACGATCGACCCTTCGCTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGAC                                                                             <  SRR4302547.444340/94‑1 (MQ=60)
                  ATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGAC                                                                             <  SRR4302547.4856843/94‑1 (MQ=60)
                    TGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGA                                                                           <  SRR4302547.3638251/94‑1 (MQ=60)
                    TGATCGTCTGGCCATTGACCACGATCAACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGA                                                                           <  SRR4302547.2216429/94‑1 (MQ=60)
                     GATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGAT                                                                          >  SRR4302547.3159609/1‑94 (MQ=60)
                     GATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGAT                                                                          <  SRR4302547.5704923/94‑1 (MQ=60)
                      ATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATC                                                                         <  SRR4302547.5671056/94‑1 (MQ=60)
                      ATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATC                                                                         >  SRR4302547.2538761/1‑94 (MQ=60)
                       TCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCC                                                                        <  SRR4302547.4812770/94‑1 (MQ=60)
                       TCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCC                                                                        <  SRR4302547.1354242/94‑1 (MQ=60)
                        tgtcgGGCCATTGACCACGATCGGCCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCG                                                                       <  SRR4302547.2300433/89‑1 (MQ=60)
                        CGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCG                                                                       >  SRR4302547.1169104/1‑94 (MQ=60)
                        CGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCG                                                                       <  SRR4302547.1606043/94‑1 (MQ=60)
                        CGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCG                                                                       <  SRR4302547.110692/94‑1 (MQ=60)
                         GTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGA                                                                      >  SRR4302547.2896339/1‑94 (MQ=60)
                           CTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATG                                                                    <  SRR4302547.762286/94‑1 (MQ=60)
                           CTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATG                                                                    <  SRR4302547.3203650/94‑1 (MQ=60)
                            TGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGA                                                                   >  SRR4302547.1759562/1‑94 (MQ=60)
                            TGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGA                                                                   >  SRR4302547.4100406/1‑94 (MQ=60)
                              GCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATG                                                                 <  SRR4302547.875336/94‑1 (MQ=60)
                               CCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGC                                                                >  SRR4302547.2767855/1‑94 (MQ=60)
                                CATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCT                                                               >  SRR4302547.4659891/1‑94 (MQ=60)
                                 ATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTT                                                              <  SRR4302547.2254828/94‑1 (MQ=60)
                                 ATCGACCACGATCGTCCCTTCGCTCGGTTTTACGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGAGCCCGACGATCCGATGATGCTT                                                              <  SRR4302547.4084756/94‑1 (MQ=60)
                                  TTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTA                                                             <  SRR4302547.3963537/94‑1 (MQ=60)
                                   TGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTAT                                                            <  SRR4302547.3047661/94‑1 (MQ=60)
                                   TGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTAT                                                            >  SRR4302547.5667204/1‑94 (MQ=60)
                                    GACCACGATCGACCCTTCACTCGGTTTTTCGTGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGACGATGCTTATT                                                           >  SRR4302547.3388963/1‑94 (MQ=60)
                                    GACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATT                                                           >  SRR4302547.3010930/1‑94 (MQ=60)
                                     ACCACGATCGTCCCTGCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTA                                                          <  SRR4302547.3655709/94‑1 (MQ=60)
                                      CCACGATCGCCCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTAC                                                         <  SRR4302547.1413267/94‑1 (MQ=60)
                                      CCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTAC                                                         <  SRR4302547.2790926/94‑1 (MQ=60)
                                       CACGATCCACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACA                                                        >  SRR4302547.4653366/1‑94 (MQ=60)
                                          GATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGTAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCT                                                     >  SRR4302547.1232723/1‑94 (MQ=60)
                                          GATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCT                                                     <  SRR4302547.1275884/94‑1 (MQ=60)
                                           ATCGCCCCTTCACGCGGTTTTTCGAGGAAGTTAATGCGGGTCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTC                                                    <  SRR4302547.2728818/94‑1 (MQ=60)
                                           ATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTC                                                    <  SRR4302547.1172863/94‑1 (MQ=60)
                                           ATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTC                                                    <  SRR4302547.6437225/94‑1 (MQ=60)
                                           ATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTC                                                    <  SRR4302547.5254878/94‑1 (MQ=60)
                                            TCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCC                                                   <  SRR4302547.5316110/94‑1 (MQ=60)
                                             CGACCCTTCACTCGGTTTTTGGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGACCCCGACGATCCGATGATCCTTATTCCATCTCCG                                                  <  SRR4302547.1796807/94‑1 (MQ=60)
                                             CGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCG                                                  <  SRR4302547.1638813/94‑1 (MQ=60)
                                              GACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGG                                                 >  SRR4302547.938713/1‑94 (MQ=60)
                                               ACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGC                                                >  SRR4302547.728044/1‑94 (MQ=60)
                                               ACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGGGCTTTTCTCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGC                                                >  SRR4302547.2387585/1‑94 (MQ=60)
                                                CCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCA                                               <  SRR4302547.5077657/94‑1 (MQ=60)
                                                CCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCA                                               <  SRR4302547.3983599/94‑1 (MQ=60)
                                                  CTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATT                                             >  SRR4302547.6182546/1‑94 (MQ=60)
                                                  CTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATT                                             <  SRR4302547.4633980/94‑1 (MQ=60)
                                                  CTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATCACATCTCCGGCATT                                             <  SRR4302547.3242795/94‑1 (MQ=60)
                                                     CACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGC                                          >  SRR4302547.1082097/1‑94 (MQ=60)
                                                     CACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGC                                          >  SRR4302547.2131478/1‑94 (MQ=60)
                                                     CACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGC                                          <  SRR4302547.1966818/94‑1 (MQ=60)
                                                       CTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTT                                        >  SRR4302547.764163/1‑94 (MQ=60)
                                                       CTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTT                                        <  SRR4302547.5272901/94‑1 (MQ=60)
                                                       CTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTT                                        <  SRR4302547.2328181/94‑1 (MQ=60)
                                                       CTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTT                                        <  SRR4302547.957268/94‑1 (MQ=60)
                                                        TCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTG                                       <  SRR4302547.5860483/94‑1 (MQ=60)
                                                         CGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGC                                      <  SRR4302547.4818364/94‑1 (MQ=60)
                                                          GGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCA                                     >  SRR4302547.3776036/1‑94 (MQ=60)
                                                          GGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCA                                     <  SRR4302547.2837784/94‑1 (MQ=60)
                                                           GTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAG                                    <  SRR4302547.2488613/94‑1 (MQ=60)
                                                           GTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAG                                    >  SRR4302547.1141259/1‑94 (MQ=60)
                                                           GTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAG                                    <  SRR4302547.6493005/94‑1 (MQ=60)
                                                            TTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGT                                   <  SRR4302547.2645640/94‑1 (MQ=60)
                                                            TTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGT                                   <  SRR4302547.101017/94‑1 (MQ=60)
                                                             TTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTG                                  >  SRR4302547.2717752/1‑94 (MQ=60)
                                                             TTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTG                                  <  SRR4302547.3611244/94‑1 (MQ=60)
                                                             TTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTG                                  <  SRR4302547.536179/94‑1 (MQ=60)
                                                              TTTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGA                                 >  SRR4302547.1438342/1‑94 (MQ=60)
                                                               TTCGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGAT                                <  SRR4302547.4134281/94‑1 (MQ=60)
                                                                 CGAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATAC                              <  SRR4302547.861162/94‑1 (MQ=60)
                                                                  GAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACC                             >  SRR4302547.5543887/1‑94 (MQ=60)
                                                                  GAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACC                             >  SRR4302547.1020169/1‑94 (MQ=60)
                                                                  GAGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACC                             >  SRR4302547.329002/1‑94 (MQ=60)
                                                                  GAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACC                             >  SRR4302547.361965/1‑94 (MQ=60)
                                                                   AGGAAGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCC                            >  SRR4302547.1906761/1‑94 (MQ=60)
                                                                     GAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCT                          >  SRR4302547.3788133/1‑94 (MQ=60)
                                                                       AGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTT                        >  SRR4302547.6099860/1‑94 (MQ=60)
                                                                       AGTTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTT                        <  SRR4302547.4671874/94‑1 (MQ=60)
                                                                         TTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCA                      <  SRR4302547.271706/94‑1 (MQ=60)
                                                                         TTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCA                      <  SRR4302547.6355369/94‑1 (MQ=60)
                                                                         TTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCA                      >  SRR4302547.3864760/1‑94 (MQ=60)
                                                                         TTAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCA                      <  SRR4302547.5267443/94‑1 (MQ=60)
                                                                         TTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCA                      <  SRR4302547.676688/94‑1 (MQ=60)
                                                                          TAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAG                     <  SRR4302547.2660619/94‑1 (MQ=60)
                                                                          TAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAG                     >  SRR4302547.6365175/1‑94 (MQ=60)
                                                                          TAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAG                     >  SRR4302547.5250948/1‑94 (MQ=60)
                                                                          TAATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAG                     >  SRR4302547.5611811/1‑94 (MQ=60)
                                                                           AATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGC                    >  SRR4302547.6442736/1‑94 (MQ=60)
                                                                           AATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGC                    >  SRR4302547.277801/1‑94 (MQ=60)
                                                                           AATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGC                    <  SRR4302547.3726168/94‑1 (MQ=60)
                                                                            ATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCA                   <  SRR4302547.1237533/94‑1 (MQ=60)
                                                                            ATGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCA                   >  SRR4302547.1844349/1‑94 (MQ=60)
                                                                             TGCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCAC                  >  SRR4302547.5876929/1‑94 (MQ=60)
                                                                              GCAGCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACT                 <  SRR4302547.824137/94‑1 (MQ=60)
                                                                                 GCGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCA              <  SRR4302547.5809740/94‑1 (MQ=60)
                                                                                  CGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCAT             <  SRR4302547.3268966/94‑1 (MQ=60)
                                                                                  CGCAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCAT             <  SRR4302547.1698015/94‑1 (MQ=60)
                                                                                   GAAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATG            <  SRR4302547.1390408/94‑1 (MQ=60)
                                                                                    CAGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGT           <  SRR4302547.656438/94‑1 (MQ=60)
                                                                                     AGAAAGTTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTT          <  SRR4302547.5745524/94‑1 (MQ=60)
                                                                                     AGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTT          <  SRR4302547.5323350/94‑1 (MQ=60)
                                                                                     AGAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTT          <  SRR4302547.3833414/94‑1 (MQ=60)
                                                                                      GAAAGTTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTC         <  SRR4302547.3722096/94‑1 (MQ=60)
                                                                                      GAAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTC         <  SRR4302547.1058042/94‑1 (MQ=60)
                                                                                       AAAGGTGCTTTTCGCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCG        >  SRR4302547.5419541/1‑94 (MQ=60)
                                                                                       AAAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCG        <  SRR4302547.5825163/94‑1 (MQ=60)
                                                                                       AAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCG        >  SRR4302547.1861891/1‑94 (MQ=60)
                                                                                        AAGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGC       >  SRR4302547.1536845/1‑94 (MQ=60)
                                                                                         AGGTGCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCC      <  SRR4302547.5273420/94‑1 (MQ=60)
                                                                                             GCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAG  <  SRR4302547.2952471/94‑1 (MQ=60)
                                                                                             GCTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAG  <  SRR4302547.1627899/94‑1 (MQ=60)
                                                                                             ACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAG  <  SRR4302547.4453144/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTCTTTGTCGCGCACCAGATTGATCGTCTGGCCATTGACCACGATCGACCCTTCACTCGGTTTTTCGAGGAAGTTAATGCAGCGCAGAAAGGTACTTTTCCCCGATCCCGACGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAG  >  NC_000913/2422302‑2422488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑