Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,667,330 T→C S93S (AGT→AGC)  hcaA1 → large terminal subunit of phenylpropionate dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,667,3300TC87.7% 640.1 / 62.7 228S93S (AGT→AGC) hcaA1large terminal subunit of phenylpropionate dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (18/10);  new base C (90/110);  total (108/120)
Fisher's exact test for biased strand distribution p-value = 6.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 230 total aligned reads displayed.

GGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTG  >  NC_000913/2667240‑2667420
                                                                                          |                                                                                          
gggAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTa                                                                                          <  1:2648627/93‑1 (MQ=255)
 ggAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTAt                                                                                         <  1:2139318/93‑1 (MQ=255)
  gagaAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATg                                                                                        >  2:6200293/1‑93 (MQ=255)
    gaagaTGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCa                                                                                      <  1:2255736/93‑1 (MQ=255)
    gaagaTGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCa                                                                                      <  1:1449631/93‑1 (MQ=255)
     aagaTGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAg                                                                                     >  1:2762097/1‑93 (MQ=255)
       gaTGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGAt                                                                                   <  2:4535125/93‑1 (MQ=255)
       gaTGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGAt                                                                                   <  2:5365152/93‑1 (MQ=255)
         tGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTg                                                                                 <  2:3168600/93‑1 (MQ=255)
         tGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTg                                                                                 >  2:607690/1‑93 (MQ=255)
          gCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGc                                                                                <  2:2864871/93‑1 (MQ=255)
          gCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGc                                                                                >  2:2713560/1‑93 (MQ=255)
          gCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGc                                                                                >  1:2215718/1‑93 (MQ=255)
          gCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGc                                                                                <  2:6433461/93‑1 (MQ=255)
            ggTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCgg                                                                              >  2:254567/1‑93 (MQ=255)
            ggTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCgg                                                                              <  1:1695899/93‑1 (MQ=255)
            ggTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCgg                                                                              <  1:790635/93‑1 (MQ=255)
             gTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGc                                                                             <  1:4552092/93‑1 (MQ=255)
                 tCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAaca                                                                         >  1:5821700/1‑93 (MQ=255)
                  cGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAacac                                                                        >  1:2887889/1‑93 (MQ=255)
                   gTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACt                                                                       >  2:2090920/1‑93 (MQ=255)
                   gTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACt                                                                       <  2:4181628/93‑1 (MQ=255)
                    tAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTc                                                                      >  2:1653859/1‑93 (MQ=255)
                    tAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTc                                                                      >  1:4767506/1‑93 (MQ=255)
                     aGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTcg                                                                     <  1:1192278/93‑1 (MQ=255)
                        gCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCgcgc                                                                  >  1:4240489/1‑93 (MQ=255)
                        gCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCgcgc                                                                  >  2:4382084/1‑93 (MQ=255)
                        gCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCgcgc                                                                  <  2:6633022/93‑1 (MQ=255)
                         cGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGcc                                                                 <  1:3982835/93‑1 (MQ=255)
                         cGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGcc                                                                 >  1:3072449/1‑93 (MQ=255)
                          gTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCt                                                                <  1:1713544/93‑1 (MQ=255)
                           tCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCtt                                                               <  2:2828654/93‑1 (MQ=255)
                           tCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCtt                                                               <  2:5953737/93‑1 (MQ=255)
                             aGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTa                                                             >  1:1760398/1‑93 (MQ=255)
                             aGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTa                                                             >  2:1455352/1‑93 (MQ=255)
                              gAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTAc                                                            >  1:130690/1‑93 (MQ=255)
                                aaGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCt                                                          >  1:1602023/1‑93 (MQ=255)
                                 aGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTg                                                         >  2:2594009/1‑93 (MQ=255)
                                  aaCGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGc                                                        <  2:4034876/92‑1 (MQ=255)
                                   aCGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGcc                                                       <  2:1870760/93‑1 (MQ=255)
                                     ggCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCg                                                     >  1:1144313/1‑93 (MQ=255)
                                       cagcaTCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTa                                                   >  2:3890441/1‑93 (MQ=255)
                                        agcaTCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTAt                                                  <  2:5169250/93‑1 (MQ=255)
                                         gcaTCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATc                                                 >  1:3332855/1‑93 (MQ=255)
                                         gcaTCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATc                                                 >  2:1087647/1‑93 (MQ=255)
                                           aTCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCAc                                               <  2:439966/93‑1 (MQ=255)
                                            tCAAGGCGTTTCTCAACCAATTCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACg                                              <  2:3342555/93‑1 (MQ=255)
                                            tCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACg                                              >  1:35478/1‑93 (MQ=255)
                                            tCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACg                                              >  2:598237/1‑93 (MQ=255)
                                            tCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACg                                              >  1:5581819/1‑93 (MQ=255)
                                            tCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACg                                              <  2:1196553/93‑1 (MQ=255)
                                             cAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACgg                                             >  2:38028/1‑93 (MQ=255)
                                              aaGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGc                                            >  2:4023470/1‑93 (MQ=255)
                                              aaGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGc                                            <  1:2035056/93‑1 (MQ=255)
                                              aaGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGc                                            <  1:3845286/93‑1 (MQ=255)
                                               aGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCt                                           >  1:5706891/1‑93 (MQ=255)
                                               aGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCt                                           >  1:3741405/1‑93 (MQ=255)
                                                ggCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTg                                          >  2:4242766/1‑93 (MQ=255)
                                                  cGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGt                                        <  2:6506673/93‑1 (MQ=255)
                                                     ttCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCtt                                     <  1:3292301/93‑1 (MQ=255)
                                                     ttCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCtt                                     <  2:1879891/93‑1 (MQ=255)
                                                      tctcAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTa                                    <  2:2601488/93‑1 (MQ=255)
                                                       ctcAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTAt                                   <  2:1318528/93‑1 (MQ=255)
                                                         cAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATgg                                 <  2:4992923/93‑1 (MQ=255)
                                                         cAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATgg                                 >  2:5242597/1‑93 (MQ=255)
                                                          aaCCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGc                                >  1:5047929/1‑93 (MQ=255)
                                                           aCCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCa                               >  2:1177084/1‑93 (MQ=255)
                                                            ccAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCAt                              <  2:1719651/93‑1 (MQ=255)
                                                             cAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCAtt                             <  1:5454257/93‑1 (MQ=255)
                                                              aaTGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTa                            >  1:3051924/1‑93 (MQ=255)
                                                              aaTGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTa                            <  1:1163582/93‑1 (MQ=255)
                                                               aTGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTaa                           >  1:2403890/1‑93 (MQ=255)
                                                                  ccgccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACgg                        <  2:5676385/93‑1 (MQ=255)
                                                                  ccgccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACgg                        >  1:1915890/1‑93 (MQ=255)
                                                                  ccgccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACgg                        <  1:5764266/93‑1 (MQ=255)
                                                                   cgccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGc                       >  2:5543567/1‑93 (MQ=255)
                                                                   cgccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGc                       >  2:4287995/1‑93 (MQ=255)
                                                                   cgccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGc                       <  1:981245/93‑1 (MQ=255)
                                                                    gccACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGACCGTATCACGGCTGGTCTTATGGCATTAACGGCg                      >  1:1068819/1‑93 (MQ=255)
                                                                    gccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGGATTAACGGCg                      >  2:5831965/1‑93 (MQ=255)
                                                                     ccACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGa                     <  2:942214/93‑1 (MQ=255)
                                                                     ccACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGa                     <  2:2980537/93‑1 (MQ=255)
                                                                     caaCCGGGCGATGCGTGTGAGCGATGCAGATTGCGGCAACACTCGCGCCTTTGCCTGCGCGTATCACGGCTGGTCTTATGGCATTAACGGCGa                     <  2:2208702/91‑1 (MQ=255)
                                                                       cccGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGt                   <  2:2354338/92‑1 (MQ=255)
                                                                       aCCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGt                   >  1:6233589/1‑93 (MQ=255)
                                                                       aCCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGt                   <  2:5122847/93‑1 (MQ=255)
                                                                        ccGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGtt                  <  2:562135/93‑1 (MQ=255)
                                                                         cGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGttt                 >  1:5945349/1‑92 (MQ=255)
                                                                         cGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTg                 >  2:3252627/1‑93 (MQ=255)
                                                                          gggCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGa                <  2:3523940/93‑1 (MQ=255)
                                                                           ggCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGAt               <  2:793140/93‑1 (MQ=255)
                                                                           ggCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGAt               >  1:2637906/1‑93 (MQ=255)
                                                                            gCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTNTGGCATTAACGGAGAGTTGNTc              >  2:5471721/1‑93 (MQ=255)
                                                                            gCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATc              >  2:167076/1‑93 (MQ=255)
                                                                             ccATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCg             <  1:4913374/93‑1 (MQ=255)
                                                                                tGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTTTGGCTTTAAAGGCGAGTTGATCGATg          >  1:6618559/1‑93 (MQ=255)
                                                                                tGCGTGTGAGCTATGCAGATTGCAGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATg          <  2:834581/93‑1 (MQ=255)
                                                                                 gCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGt         <  1:2020661/93‑1 (MQ=255)
                                                                                 gCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGGTGATCGATGt         >  1:2855680/1‑93 (MQ=255)
                                                                                   gtgtGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTAc       >  1:6524266/1‑93 (MQ=255)
                                                                                          |                                                                                          
GGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTG  >  NC_000913/2667240‑2667420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGA  >  NC_000913/2667237‑2667422
                                                                                             |                                                                                            
CATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGC                                                                                              >  SRR4302547.2651837/1‑94 (MQ=60)
 ATGGGAGAGGATGCGGTTGTCGTAGTGCGTGAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAAGGCCTCCACCGGGCGATGCGTGTGAGCT                                                                                             <  SRR4302547.1640969/94‑1 (MQ=60)
 ATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCT                                                                                             <  SRR4302547.5921099/94‑1 (MQ=60)
  TGGGAGAGGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGGGTGTGAGCTA                                                                                            <  SRR4302547.3574877/94‑1 (MQ=60)
  TGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTA                                                                                            >  SRR4302547.3363783/1‑94 (MQ=60)
   GGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTAT                                                                                           <  SRR4302547.2701902/94‑1 (MQ=60)
    GGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATG                                                                                          <  SRR4302547.2687895/94‑1 (MQ=60)
    GGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATG                                                                                          <  SRR4302547.2181977/94‑1 (MQ=60)
     GAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCAGCCACCGGGCCATGCGTGTGAGCTATGC                                                                                         >  SRR4302547.5077530/1‑94 (MQ=60)
       GAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAG                                                                                       <  SRR4302547.2301290/94‑1 (MQ=60)
       GAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAG                                                                                       <  SRR4302547.1478620/94‑1 (MQ=60)
       GAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAG                                                                                       >  SRR4302547.2817451/1‑94 (MQ=60)
         AGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGAT                                                                                     >  SRR4302547.1233416/1‑94 (MQ=60)
         AGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGAT                                                                                     >  SRR4302547.2767117/1‑94 (MQ=60)
         AGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGAT                                                                                     <  SRR4302547.1356162/94‑1 (MQ=60)
          GATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATG                                                                                    >  SRR4302547.1330725/1‑94 (MQ=60)
           ATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTG                                                                                   >  SRR4302547.6745328/1‑94 (MQ=60)
            TGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGC                                                                                  <  SRR4302547.5069075/94‑1 (MQ=60)
            TGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGC                                                                                  >  SRR4302547.2260332/1‑94 (MQ=60)
              CGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGG                                                                                <  SRR4302547.5718872/94‑1 (MQ=60)
               GGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGC                                                                               <  SRR4302547.1729245/94‑1 (MQ=60)
               GGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGC                                                                               <  SRR4302547.2234229/94‑1 (MQ=60)
               GGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGC                                                                               <  SRR4302547.805599/94‑1 (MQ=60)
                GTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCA                                                                              <  SRR4302547.3668836/94‑1 (MQ=60)
                GTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCA                                                                              <  SRR4302547.4646248/94‑1 (MQ=60)
                  TGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAAC                                                                            >  SRR4302547.2213947/1‑94 (MQ=60)
                   GTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACA                                                                           >  SRR4302547.5942139/1‑94 (MQ=60)
                   GTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACA                                                                           >  SRR4302547.4958445/1‑94 (MQ=60)
                    TCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACAC                                                                          >  SRR4302547.2945505/1‑94 (MQ=60)
                      GTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTC                                                                        >  SRR4302547.206945/1‑94 (MQ=60)
                      GTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTC                                                                        >  SRR4302547.4866275/1‑94 (MQ=60)
                       TAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCG                                                                       <  SRR4302547.935768/94‑1 (MQ=60)
                        AGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGC                                                                      <  SRR4302547.1216339/94‑1 (MQ=60)
                          TGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGC                                                                    >  SRR4302547.4327201/1‑94 (MQ=60)
                           GCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCC                                                                   >  SRR4302547.3133483/1‑94 (MQ=60)
                            CGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCT                                                                  <  SRR4302547.4063824/94‑1 (MQ=60)
                             GTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTT                                                                 <  SRR4302547.1747243/94‑1 (MQ=60)
                              TCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTT                                                                <  SRR4302547.2857178/94‑1 (MQ=60)
                               CAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTA                                                               >  SRR4302547.1795295/1‑94 (MQ=60)
                               CAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTA                                                               >  SRR4302547.4024693/1‑94 (MQ=60)
                                AGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTAC                                                              >  SRR4302547.133054/1‑94 (MQ=60)
                                 GAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACC                                                             <  SRR4302547.2701394/94‑1 (MQ=60)
                                  acaggCGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCCTGTGATCTATGCAGATTGCGGCAACACTCGCGCCTTTACCT                                                            <  SRR4302547.2289995/89‑1 (MQ=60)
                                  AAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCT                                                            >  SRR4302547.1633798/1‑94 (MQ=60)
                                      ACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCC                                                        <  SRR4302547.1208344/94‑1 (MQ=60)
                                       CGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCG                                                       >  SRR4302547.1167440/1‑94 (MQ=60)
                                         GCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTA                                                     >  SRR4302547.5942196/1‑94 (MQ=60)
                                           AGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATC                                                   >  SRR4302547.3400258/1‑94 (MQ=60)
                                           AGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATC                                                   >  SRR4302547.5834485/1‑94 (MQ=60)
                                            GCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCA                                                  <  SRR4302547.4801991/94‑1 (MQ=60)
                                             CATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCAC                                                 >  SRR4302547.2327489/1‑94 (MQ=60)
                                              ATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACG                                                >  SRR4302547.36125/1‑94 (MQ=60)
                                              ATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACG                                                >  SRR4302547.5696949/1‑94 (MQ=60)
                                                CAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGGGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGC                                              >  SRR4302547.3892792/1‑94 (MQ=60)
                                                CAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGC                                              >  SRR4302547.5245766/1‑94 (MQ=60)
                                                 AAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCT                                             <  SRR4302547.2075605/94‑1 (MQ=60)
                                                 AAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCT                                             >  SRR4302547.5824779/1‑94 (MQ=60)
                                                 AAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCT                                             >  SRR4302547.3817781/1‑94 (MQ=60)
                                                 AAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCT                                             <  SRR4302547.3923578/94‑1 (MQ=60)
                                                  AGTCGTTTCTCAGCCAATGCCGCCACTGGGCCATGCGTGTAAGTTATGCAGATTGCGGCAACACTCGCGGCTTTACCTGCCCGTATCACGGCTG                                            <  SRR4302547.4284669/94‑1 (MQ=60)
                                                  AGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTG                                            <  SRR4302547.4390698/94‑1 (MQ=60)
                                                      GTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCT                                        <  SRR4302547.2275380/94‑1 (MQ=60)
                                                      GTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCT                                        <  SRR4302547.3401941/94‑1 (MQ=60)
                                                       TTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTT                                       <  SRR4302547.4220605/94‑1 (MQ=60)
                                                        TTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTA                                      <  SRR4302547.3358429/94‑1 (MQ=60)
                                                         TCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTAT                                     <  SRR4302547.4305881/94‑1 (MQ=60)
                                                          TTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATG                                    <  SRR4302547.1175177/94‑1 (MQ=60)
                                                           TCAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGG                                   <  SRR4302547.6154416/94‑1 (MQ=60)
                                                            CAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGC                                  >  SRR4302547.2919926/1‑94 (MQ=60)
                                                            CAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGC                                  >  SRR4302547.5152152/1‑94 (MQ=60)
                                                            CAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGC                                  >  SRR4302547.796100/1‑94 (MQ=60)
                                                            CAACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGAGCCTTTACCTGCCCGTATCAAGGCTGGGCTTGTGGC                                  >  SRR4302547.1899843/1‑94 (MQ=60)
                                                             AACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCA                                 <  SRR4302547.1909951/94‑1 (MQ=60)
                                                             AACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCA                                 >  SRR4302547.5697533/1‑94 (MQ=60)
                                                             AACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCA                                 >  SRR4302547.6012893/1‑94 (MQ=60)
                                                              ACCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCAT                                >  SRR4302547.5887712/1‑94 (MQ=60)
                                                               CCAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATT                               >  SRR4302547.1739746/1‑94 (MQ=60)
                                                                CAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTA                              <  SRR4302547.5566959/94‑1 (MQ=60)
                                                                CAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTA                              <  SRR4302547.2028146/94‑1 (MQ=60)
                                                                CAATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTA                              >  SRR4302547.3112667/1‑94 (MQ=60)
                                                                 AATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAA                             >  SRR4302547.2452573/1‑94 (MQ=60)
                                                                 AATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAA                             >  SRR4302547.2973714/1‑94 (MQ=60)
                                                                 AATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAA                             <  SRR4302547.1187122/94‑1 (MQ=60)
                                                                 AATGCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAA                             <  SRR4302547.5664999/94‑1 (MQ=60)
                                                                    GCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGG                          >  SRR4302547.1953827/1‑94 (MQ=60)
                                                                    GCCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGG                          <  SRR4302547.1143123/94‑1 (MQ=60)
                                                                     CCGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGC                         <  SRR4302547.5883477/94‑1 (MQ=60)
                                                                      CGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGACCGTATCACGGCTGGTCTTATGGCATTAACGGCG                        >  SRR4302547.1090101/1‑94 (MQ=60)
                                                                      CGCCACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCG                        <  SRR4302547.1000338/94‑1 (MQ=60)
                                                                         CACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGT                     >  SRR4302547.3853156/1‑94 (MQ=60)
                                                                         CACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGT                     >  SRR4302547.6363088/1‑94 (MQ=60)
                                                                          ACCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTT                    <  SRR4302547.1689988/94‑1 (MQ=60)
                                                                           CCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGTGAGGTG                   >  SRR4302547.1584457/1‑94 (MQ=60)
                                                                           CCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTT                   >  SRR4302547.6068633/1‑94 (MQ=60)
                                                                           CCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTG                   <  SRR4302547.679348/94‑1 (MQ=60)
                                                                           CCGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTG                   <  SRR4302547.3197170/94‑1 (MQ=60)
                                                                            CGGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGA                  <  SRR4302547.3132745/94‑1 (MQ=60)
                                                                             GGGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGAT                 >  SRR4302547.2691014/1‑94 (MQ=60)
                                                                              GGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATC                <  SRR4302547.3307354/94‑1 (MQ=60)
                                                                              GGCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATC                <  SRR4302547.2087169/94‑1 (MQ=60)
                                                                               GCCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCG               <  SRR4302547.2498924/94‑1 (MQ=60)
                                                                                CCATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGA              <  SRR4302547.5015117/94‑1 (MQ=60)
                                                                                 CATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACTGGGAGTTGATCGAT             >  SRR4302547.1688659/1‑94 (MQ=60)
                                                                                  ATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCAAGTTGATCGATG            >  SRR4302547.1755480/1‑94 (MQ=60)
                                                                                  ATGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTTTGGCTTTAAAGGCGAGTTGATCGATG            >  SRR4302547.6756313/1‑94 (MQ=60)
                                                                                   TGCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGGTGATCGATGT           >  SRR4302547.2912740/1‑94 (MQ=60)
                                                                                    GCGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTA          <  SRR4302547.2060877/94‑1 (MQ=60)
                                                                                     CGTGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTAC         >  SRR4302547.6660088/1‑94 (MQ=60)
                                                                                       TGTGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCG       <  SRR4302547.2856316/94‑1 (MQ=60)
                                                                                         TGAGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGCTGATCTATGTACCGCT     >  SRR4302547.654563/1‑94 (MQ=60)
                                                                                          GAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTG    >  SRR4302547.3726935/1‑94 (MQ=60)
                                                                                           AGCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGG   <  SRR4302547.5599700/94‑1 (MQ=60)
                                                                                            GCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGA  >  SRR4302547.336001/1‑94 (MQ=60)
                                                                                            GCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGA  <  SRR4302547.715944/94‑1 (MQ=60)
                                                                                            GCTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGA  <  SRR4302547.3525877/94‑1 (MQ=60)
                                                                                             |                                                                                            
CATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGA  >  NC_000913/2667237‑2667422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑