Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,427 2 bp→AC coding (697‑698/864 nt) b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4270TA99.6% 818.4 / NA 221W233T (TGG→ACG) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (106/114);  total (106/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,766,4280GC100.0% 782.0 / NA 221W233T (TGG→ACG) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (106/115);  total (106/115)

Only 100 of 229 total aligned reads displayed.

AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510
                                                                                       ||                                                                                  
aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                >  1:5429885/1‑93 (MQ=25)
aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                >  1:1502130/1‑93 (MQ=25)
aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                >  2:5584010/1‑93 (MQ=21)
 gggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAg                                                                               <  2:5237575/93‑1 (MQ=21)
   gTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                             >  1:5633189/1‑93 (MQ=21)
      aTGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg                                                                          <  1:4494597/93‑1 (MQ=21)
       tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                         >  1:1899690/1‑93 (MQ=25)
          cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGc                                                                      >  2:5327918/1‑93 (MQ=25)
           agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                     <  1:5033782/93‑1 (MQ=25)
           agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                     <  1:6141223/93‑1 (MQ=25)
            gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg                                                                    <  1:352984/93‑1 (MQ=25)
            gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg                                                                    >  2:1024563/1‑93 (MQ=21)
             agCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAgg                                                                   >  2:2511042/1‑93 (MQ=25)
              gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc                                                                  >  1:2926577/1‑93 (MQ=25)
              gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc                                                                  >  1:2843644/1‑93 (MQ=25)
              gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc                                                                  <  2:3002251/93‑1 (MQ=25)
               cGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCa                                                                 <  2:4961760/93‑1 (MQ=255)
               cGGATGATTAAGTGCCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCa                                                                 <  2:5042244/93‑1 (MQ=21)
                ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGTGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                >  2:5874254/1‑93 (MQ=255)
                  atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGt                                                              <  1:2215297/93‑1 (MQ=255)
                  atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGt                                                              <  1:1348566/93‑1 (MQ=255)
                  atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGt                                                              <  2:868653/93‑1 (MQ=255)
                   tgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTg                                                             <  2:5959508/93‑1 (MQ=255)
                    gatTAAGTGTCTGCGGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa                                                            >  1:1115152/1‑93 (MQ=255)
                    gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa                                                            >  2:1798265/1‑93 (MQ=255)
                     atTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAc                                                           >  1:2829705/1‑93 (MQ=255)
                      ttAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACg                                                          <  2:2331628/93‑1 (MQ=255)
                       tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGa                                                         >  1:526696/1‑93 (MQ=255)
                        aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAt                                                        <  1:3740179/93‑1 (MQ=255)
                         aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                       >  1:117514/1‑93 (MQ=255)
                         aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                       <  2:3542297/93‑1 (MQ=255)
                         aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                       <  2:422119/93‑1 (MQ=255)
                          gtgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAttt                                                      >  2:630950/1‑93 (MQ=255)
                          gtgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAttt                                                      <  1:4890284/93‑1 (MQ=255)
                           tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc                                                     <  2:3843542/93‑1 (MQ=255)
                           tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc                                                     <  1:4788861/93‑1 (MQ=255)
                           tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc                                                     <  1:1199860/93‑1 (MQ=255)
                              cTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGt                                                  <  1:4059332/93‑1 (MQ=255)
                               tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTg                                                 <  1:2493555/93‑1 (MQ=255)
                               tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTg                                                 >  2:5094518/1‑93 (MQ=255)
                                 ccGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                               >  1:3333401/1‑93 (MQ=255)
                                 ccGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                               <  1:781097/93‑1 (MQ=255)
                                  cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                              <  1:5687370/93‑1 (MQ=255)
                                  cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                              >  2:2433811/1‑93 (MQ=255)
                                   gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                             >  1:6376029/1‑93 (MQ=255)
                                    cgTGAGGCCAGCAGCCCGGTGGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                            <  1:3792549/93‑1 (MQ=255)
                                    cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                            <  1:2762217/93‑1 (MQ=255)
                                     gTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCg                                           >  2:2551452/1‑93 (MQ=255)
                                        aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                        <  2:1909887/90‑1 (MQ=255)
                                         ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg                                       <  2:4868200/91‑2 (MQ=255)
                                              gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                  >  2:5816849/1‑93 (MQ=255)
                                                agcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa                                <  1:3663741/93‑1 (MQ=255)
                                                 gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                               >  1:3437354/1‑93 (MQ=255)
                                                 gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                               <  2:4582067/93‑1 (MQ=255)
                                                 gcCCGGGAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                               >  2:4392575/1‑93 (MQ=255)
                                                   ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                             >  1:463347/1‑93 (MQ=255)
                                                   ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                             <  2:1103830/93‑1 (MQ=255)
                                                    cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                            >  2:1455657/1‑93 (MQ=255)
                                                    cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                            >  1:4984517/1‑93 (MQ=255)
                                                    cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                            >  2:3455395/1‑93 (MQ=255)
                                                     ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGCAACCGTAGGTGCCATACTCGa                           >  2:2833763/1‑93 (MQ=255)
                                                     ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                           <  1:2466369/93‑1 (MQ=255)
                                                      gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                          <  2:1240114/93‑1 (MQ=255)
                                                       tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                         <  1:589069/93‑1 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAACCCGTAGGTGCCATACTCGATAc                        <  1:1951513/93‑1 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                        >  2:4106865/1‑93 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                        <  1:5992325/93‑1 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                        <  2:1350677/93‑1 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                        >  1:2781869/1‑93 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                        <  1:4040433/93‑1 (MQ=255)
                                                          tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                      <  2:719229/93‑1 (MQ=255)
                                                          tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                      <  2:3281013/93‑1 (MQ=255)
                                                             cgcTACTGCAACATCCCTTTCGTACAACGGTAGCCCGGGGGATGGCGCGTGACGATTTCGGTGAATCCGTTGGTGCCATACTCGATACGGTaa                   <  1:6649862/93‑1 (MQ=255)
                                                                 aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc               >  1:2185325/1‑93 (MQ=255)
                                                                 aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc               >  2:6414984/1‑93 (MQ=255)
                                                                 aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc               >  2:2218277/1‑93 (MQ=255)
                                                                  cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAcc              <  2:1586306/93‑1 (MQ=255)
                                                                  cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAcc              >  2:2077489/1‑93 (MQ=255)
                                                                   tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt             <  2:4011187/93‑1 (MQ=255)
                                                                   tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt             <  1:862675/93‑1 (MQ=255)
                                                                   tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt             <  2:5487743/93‑1 (MQ=255)
                                                                   tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt             <  1:3089881/93‑1 (MQ=255)
                                                                   cgCAATATCCCTTTCGACAAACGGTAGCCCGGCACCAGGCCCGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt             <  2:6567522/92‑1 (MQ=255)
                                                                    gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt            <  1:2538831/93‑1 (MQ=255)
                                                                    gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt            >  1:5185912/1‑93 (MQ=255)
                                                                    gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt            <  2:3412415/93‑1 (MQ=255)
                                                                    acaccATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGCCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt            <  1:2298006/89‑1 (MQ=255)
                                                                      aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc          <  2:465350/93‑1 (MQ=255)
                                                                       aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc         >  2:4721156/1‑93 (MQ=255)
                                                                       aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc         >  2:4415338/1‑93 (MQ=255)
                                                                       aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc         <  2:6378568/93‑1 (MQ=255)
                                                                        cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc        <  2:2201595/93‑1 (MQ=255)
                                                                        cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc        <  2:1422976/93‑1 (MQ=255)
                                                                        cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc        >  1:6297958/1‑93 (MQ=255)
                                                                         aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc       >  1:1100030/1‑93 (MQ=255)
                                                                          tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccctt      <  1:6549375/93‑3 (MQ=255)
                                                                           cccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt     >  1:4414482/1‑93 (MQ=255)
                                                                            ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa    <  2:4479472/93‑1 (MQ=255)
                                                                            ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa    <  2:3188090/93‑1 (MQ=255)
                                                                              tttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt  <  2:1962148/93‑1 (MQ=255)
                                                                                       ||                                                                                  
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510
                                                                                       |                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               <  SRR4302547.1705997/94‑1 (MQ=60)
  GGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCC                                                                             <  SRR4302547.4548716/94‑1 (MQ=60)
  GGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCC                                                                             >  SRR4302547.5749444/1‑94 (MQ=60)
   GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            <  SRR4302547.5032963/94‑1 (MQ=60)
   GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            >  SRR4302547.5471660/1‑94 (MQ=60)
   GAGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCACTTTCGAACATCGGTAGCCC                                                                            <  SRR4302547.6586853/94‑1 (MQ=60)
     GATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGG                                                                          >  SRR4302547.2885170/1‑94 (MQ=60)
      ATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGG                                                                         <  SRR4302547.4587429/94‑1 (MQ=60)
      ATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGG                                                                         >  SRR4302547.239576/1‑94 (MQ=60)
      ATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGG                                                                         >  SRR4302547.1937277/1‑94 (MQ=60)
          CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCA                                                                     >  SRR4302547.2088702/1‑94 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302547.1220847/94‑1 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302547.5137776/94‑1 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302547.6268619/94‑1 (MQ=60)
            GAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGG                                                                   <  SRR4302547.359538/94‑1 (MQ=60)
            GAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGG                                                                   >  SRR4302547.5132144/1‑94 (MQ=60)
             AGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGC                                                                  >  SRR4302547.2900465/1‑94 (MQ=60)
             AGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGC                                                                  >  SRR4302547.2984944/1‑94 (MQ=60)
                GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302547.2682649/1‑94 (MQ=60)
                GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               <  SRR4302547.1870822/94‑1 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             >  SRR4302547.6434726/1‑94 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             <  SRR4302547.2259853/94‑1 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             <  SRR4302547.1375661/94‑1 (MQ=60)
                   TGATTAAGTGTCTGCGGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGA                                                            >  SRR4302547.1137605/1‑94 (MQ=60)
                   TGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGA                                                            >  SRR4302547.1968521/1‑94 (MQ=60)
                    GATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAC                                                           <  SRR4302547.2970516/94‑1 (MQ=60)
                    GATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAC                                                           >  SRR4302547.2886289/1‑94 (MQ=60)
                     ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACG                                                          <  SRR4302547.2439854/94‑1 (MQ=60)
                      TTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGA                                                         >  SRR4302547.536221/1‑94 (MQ=60)
                      TTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGA                                                         >  SRR4302547.4087863/1‑94 (MQ=60)
                        AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       <  SRR4302547.3816538/94‑1 (MQ=60)
                        AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302547.119648/1‑94 (MQ=60)
                         AGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTT                                                      >  SRR4302547.3742187/1‑94 (MQ=60)
                         AGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTT                                                      <  SRR4302547.4622709/94‑1 (MQ=60)
                          GTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTC                                                     <  SRR4302547.4991568/94‑1 (MQ=60)
                           TGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCG                                                    <  SRR4302547.4888084/94‑1 (MQ=60)
                           TGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCG                                                    <  SRR4302547.1224069/94‑1 (MQ=60)
                             TCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGT                                                  <  SRR4302547.2545409/94‑1 (MQ=60)
                              CTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTG                                                 <  SRR4302547.4141771/94‑1 (MQ=60)
                              CTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTG                                                 <  SRR4302547.3693497/94‑1 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                <  SRR4302547.2543911/94‑1 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                >  SRR4302547.1362568/1‑94 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                <  SRR4302547.99083/94‑1 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                >  SRR4302547.5559482/1‑94 (MQ=60)
                                GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               >  SRR4302547.3400817/1‑94 (MQ=60)
                                 CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              <  SRR4302547.795864/94‑1 (MQ=60)
                                  CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAC                                             >  SRR4302547.6782933/1‑94 (MQ=60)
                                  CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAC                                             <  SRR4302547.5804808/94‑1 (MQ=60)
                                  CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAC                                             >  SRR4302547.6509073/1‑94 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTGGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           <  SRR4302547.3869871/94‑1 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTTACGATTTCGGTGAAACCG                                           <  SRR4302547.2533585/94‑1 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           <  SRR4302547.2817572/94‑1 (MQ=60)
                                    CGAGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           >  SRR4302547.6685642/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          <  SRR4302547.2275013/94‑1 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302547.5775337/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302547.6308851/1‑94 (MQ=60)
                                      TGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTA                                         >  SRR4302547.4158951/1‑94 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        <  SRR4302547.4986952/94‑1 (MQ=60)
                                        AGTCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGG                                       <  SRR4302547.5965288/94‑1 (MQ=60)
                                         GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      <  SRR4302547.5358412/94‑1 (MQ=60)
                                           CCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtgc                                    <  SRR4302547.4969104/94‑5 (MQ=60)
                                            CAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgggca                                   >  SRR4302547.589078/1‑89 (MQ=60)
                                             AGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCcgaggtgcca                                  >  SRR4302547.455617/1‑84 (MQ=60)
                                              GCAGCCCGGTAGTAGCGCTCCTGCAACATGCCTTCCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                 <  SRR4302547.412080/94‑1 (MQ=60)
                                               CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                >  SRR4302547.3403937/1‑94 (MQ=60)
                                                CGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               >  SRR4302547.3507439/1‑94 (MQ=60)
                                                AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               <  SRR4302547.3738483/94‑1 (MQ=60)
                                                AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               >  SRR4302547.5211697/1‑94 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302547.471903/1‑94 (MQ=60)
                                                   CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            >  SRR4302547.5087589/1‑94 (MQ=60)
                                                    CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                           <  SRR4302547.611482/94‑1 (MQ=60)
                                                     GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          <  SRR4302547.2516218/94‑1 (MQ=60)
                                                       TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302547.599732/94‑1 (MQ=60)
                                                       TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        >  SRR4302547.2837596/1‑94 (MQ=60)
                                                       TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302547.1253853/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAACCCGTAGGTGCCATACTCGATACG                       <  SRR4302547.1990219/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302547.2893149/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302547.6116655/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302547.4122483/94‑1 (MQ=60)
                                                         GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      >  SRR4302547.6081888/1‑94 (MQ=60)
                                                           AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                    >  SRR4302547.784910/1‑94 (MQ=60)
                                                           AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                    >  SRR4302547.11281/1‑94 (MQ=60)
                                                             CGCTACTGCAACATCCCTTTCGTACAACGGTAGCCCGGGGGATGGCGCGTGACGATTTCGGTGAATCCGTTGGTGCCATACTCGATACGGTAAG                  <  SRR4302547.6788430/94‑1 (MQ=32)
                                                             CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  >  SRR4302547.282581/1‑94 (MQ=60)
                                                             CGCCACTGCCACATCCCTTGCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  <  SRR4302547.3482571/94‑1 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 >  SRR4302547.1502382/1‑94 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 <  SRR4302547.3164070/94‑1 (MQ=60)
                                                               tgACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGACACGTGACGATTTCGGTGAAACCGTAGGTGCCATGCTCGATACGGTAAGCA                <  SRR4302547.2967410/92‑1 (MQ=60)
                                                               CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                >  SRR4302547.4793469/1‑94 (MQ=60)
                                                               CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                >  SRR4302547.426255/1‑94 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302547.5156322/1‑94 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302547.3817982/1‑94 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302547.2229136/1‑94 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302547.6213045/1‑94 (MQ=60)
                                                                   TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            <  SRR4302547.879163/94‑1 (MQ=60)
                                                                   TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            <  SRR4302547.3151169/94‑1 (MQ=60)
                                                                   TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            >  SRR4302547.5292643/1‑94 (MQ=60)
                                                                    acacCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGCCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           <  SRR4302547.2344579/90‑1 (MQ=60)
                                                                    GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           <  SRR4302547.2251913/94‑1 (MQ=60)
                                                                    GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           <  SRR4302547.2590119/94‑1 (MQ=60)
                                                                      AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         >  SRR4302547.5581111/1‑94 (MQ=60)
                                                                       ACATCCCTTTCGAACAACGGTAGCCCGTGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCC        <  SRR4302547.2789159/94‑1 (MQ=60)
                                                                       ACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCC        >  SRR4302547.6428873/1‑94 (MQ=60)
                                                                       ACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCA        <  SRR4302547.19582/94‑1 (MQ=60)
                                                                        CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC       >  SRR4302547.1122121/1‑94 (MQ=60)
                                                                        CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC       >  SRR4302547.1190100/1‑94 (MQ=60)
                                                                         ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT      <  SRR4302547.816130/94‑1 (MQ=60)
                                                                          TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCTTT     <  SRR4302547.6685666/94‑1 (MQ=60)
                                                                          TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTT     >  SRR4302547.4505531/1‑94 (MQ=60)
                                                                           CCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTA    <  SRR4302547.3529337/94‑1 (MQ=60)
                                                                            CCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAT   >  SRR4302547.4751593/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  <  SRR4302547.3085803/94‑1 (MQ=60)
                                                                                       |                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑