Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,839,652 C→T L214L (CTG→TTG)  ascB → 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,6520CT88.1% 617.6 / 52.7 211L214L (CTG→TTG) ascB6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (13/12);  new base T (94/91);  total (108/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01

Only 100 of 217 total aligned reads displayed.

TTATGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACC  >  NC_000913/2839560‑2839742
                                                                                            |                                                                                          
  aTGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTg                                                                                          <  2:566686/93‑1 (MQ=255)
   tGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTgg                                                                                         >  2:6620778/1‑93 (MQ=255)
    gTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGt                                                                                        >  1:1042049/1‑93 (MQ=255)
    gTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGt                                                                                        <  2:3311898/93‑1 (MQ=255)
    gTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGt                                                                                        <  1:4452261/93‑1 (MQ=255)
    gTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGt                                                                                        <  2:466267/93‑1 (MQ=255)
         aTAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCa                                                                                   >  2:1419135/1‑93 (MQ=255)
         aTAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCa                                                                                   >  2:5238993/1‑93 (MQ=255)
          tAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAg                                                                                  >  2:3743298/1‑93 (MQ=255)
          tAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAg                                                                                  >  2:1400285/1‑93 (MQ=255)
           aGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGt                                                                                 <  2:1197794/93‑1 (MQ=255)
            gCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTg                                                                                >  1:1621159/1‑93 (MQ=255)
            gCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTg                                                                                >  2:1321902/1‑93 (MQ=255)
            gCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTg                                                                                >  2:5736160/1‑93 (MQ=255)
            gCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTg                                                                                >  2:2097954/1‑93 (MQ=255)
             cccGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTgc                                                                               >  2:4824683/1‑93 (MQ=255)
             cccGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTgc                                                                               <  2:3071077/93‑1 (MQ=255)
              ccGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTgcg                                                                              >  1:860530/1‑93 (MQ=255)
               cGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTgcgc                                                                             >  1:6186903/1‑93 (MQ=255)
               cGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTgcgc                                                                             >  1:2508093/1‑93 (MQ=255)
               cGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGACGCGCATCACCAGTTGGTTGCCAGTgcgc                                                                             >  2:162378/1‑93 (MQ=255)
                gTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCt                                                                            <  2:4330039/93‑1 (MQ=255)
                gTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCt                                                                            >  2:5404292/1‑93 (MQ=255)
                gTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCt                                                                            >  2:5868784/1‑93 (MQ=255)
                gTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGACGCGCATCACCAGTTGGTTGCCAGTGCGCt                                                                            >  2:4588768/1‑93 (MQ=255)
                 ttCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGGCGAGCATGACCAGTTGGTTGCCAGTGCGCTa                                                                           >  2:4540528/1‑93 (MQ=255)
                  tctcCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGACCGCGCATCACCAGCTGGTTGCCAGTGCGCTAg                                                                          >  2:447513/1‑93 (MQ=255)
                    tcCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGcc                                                                        <  2:4804237/93‑1 (MQ=255)
                    tcCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGcc                                                                        <  1:6541772/93‑1 (MQ=255)
                      cGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGccac                                                                      >  1:1579064/1‑93 (MQ=255)
                        gcgcGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGccacca                                                                    >  1:3472572/1‑93 (MQ=255)
                           cgGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCaaaa                                                                 >  2:3922382/1‑93 (MQ=255)
                           cgGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCaaaa                                                                 <  2:362908/93‑1 (MQ=255)
                           cgGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCaaaa                                                                 >  2:2931554/1‑93 (MQ=255)
                           cgGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCaaaa                                                                 >  2:822261/1‑93 (MQ=255)
                            gggTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAAt                                                                <  1:617640/93‑1 (MQ=255)
                            gggTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAAt                                                                <  1:2040745/93‑1 (MQ=255)
                             ggTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGGGAAATATCAGGCCGGGCATAACCAGTTGTTTGCCAGGGCGCTGGCACCCAAAATc                                                               >  1:4217879/1‑93 (MQ=255)
                              gTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCg                                                              >  1:3810749/1‑93 (MQ=255)
                              gTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCg                                                              <  2:3777424/93‑1 (MQ=255)
                               tCTGGTGTTTGAAGAAGGTCAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGc                                                             >  2:2642361/1‑93 (MQ=255)
                                 tGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGccc                                                           <  2:4400340/93‑1 (MQ=255)
                                  ggTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCa                                                          >  2:2462131/1‑93 (MQ=255)
                                  ggTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCa                                                          <  2:6017492/93‑1 (MQ=255)
                                  ggTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCa                                                          <  1:3126939/93‑1 (MQ=255)
                                   gtgtTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCAt                                                         <  1:3202879/93‑1 (MQ=255)
                                      tttGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAg                                                      >  1:706039/1‑93 (MQ=255)
                                         gaagaaGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGtt                                                   <  2:4287411/93‑1 (MQ=255)
                                         gaagaaGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGtt                                                   <  2:2270288/93‑1 (MQ=255)
                                          aagaagGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTa                                                  >  2:1807462/1‑93 (MQ=255)
                                           agaagGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTaa                                                 >  1:6355696/1‑93 (MQ=255)
                                            gaagGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAAc                                                >  1:5454681/1‑93 (MQ=255)
                                            gaagGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAAc                                                >  1:1149254/1‑93 (MQ=255)
                                              agGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAAccc                                              >  1:2681010/1‑93 (MQ=255)
                                                gTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGc                                            <  1:5534893/93‑1 (MQ=255)
                                                 tGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCa                                           >  2:3700735/1‑93 (MQ=255)
                                                  gAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTCGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCaa                                          >  1:1369721/1‑93 (MQ=255)
                                                   aaaaTCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCaaa                                         >  1:4523251/1‑93 (MQ=255)
                                                   aaaaTCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGGTGGTTGCCAGTGCGCTAGCCACCAACATCGCCCATGAGGTTAACCCGCaaa                                         >  1:5734715/1‑93 (MQ=255)
                                                    aaaTCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCaaaa                                        <  1:1496530/93‑1 (MQ=255)
                                                    aaaTCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTCCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCaaaa                                        <  1:2762809/93‑1 (MQ=255)
                                                     aaTCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAAt                                       <  1:1558055/93‑1 (MQ=255)
                                                      atcaggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATc                                      <  1:5319880/93‑1 (MQ=255)
                                                      atcaggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATc                                      <  2:6469425/93‑1 (MQ=255)
                                                       tcaggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCa                                     <  1:480896/93‑1 (MQ=255)
                                                       tcaggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCa                                     <  2:399735/93‑1 (MQ=255)
                                                       tcaggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCa                                     <  2:6362206/93‑1 (MQ=255)
                                                        caggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAg                                    <  2:6169074/93‑1 (MQ=255)
                                                         aggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAgg                                   >  2:1963198/1‑93 (MQ=255)
                                                         aggatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAgg                                   <  1:3961703/93‑1 (MQ=255)
                                                          ggatcagtTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGt                                  <  2:6292954/93‑1 (MQ=255)
                                                           gatcaggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTg                                 <  2:1012126/93‑1 (MQ=255)
                                                              caggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGc                              >  1:6025824/1‑93 (MQ=255)
                                                               aggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCt                             <  2:3157909/93‑1 (MQ=255)
                                                               aggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCt                             >  2:4986959/1‑93 (MQ=255)
                                                               aggTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCt                             <  1:1938838/93‑1 (MQ=255)
                                                                 gTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGt                           <  1:235305/93‑1 (MQ=255)
                                                                  tGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTa                          >  2:3642573/1‑93 (MQ=255)
                                                                    aaaTATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATg                        <  1:3527620/93‑1 (MQ=255)
                                                                    aaaTATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATg                        >  1:1080405/1‑93 (MQ=255)
                                                                     aaTATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGc                       <  1:2192611/93‑1 (MQ=255)
                                                                     aaTATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGc                       >  2:4891175/1‑93 (MQ=255)
                                                                      atatCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCt                      >  2:5432867/1‑93 (MQ=255)
                                                                      atatCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCt                      <  1:6239076/93‑1 (MQ=255)
                                                                      atatCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCt                      >  2:5065411/1‑93 (MQ=255)
                                                                        atCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTgg                    <  1:3681877/93‑1 (MQ=255)
                                                                        atCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTgg                    >  1:3252414/1‑93 (MQ=255)
                                                                         tCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTggc                   >  2:2071095/1‑93 (MQ=255)
                                                                           aGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTggcgg                 >  1:253169/1‑93 (MQ=255)
                                                                            ggCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTggcggg                <  2:1123251/93‑1 (MQ=255)
                                                                            ggCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTggcggg                <  2:6544506/93‑1 (MQ=255)
                                                                             gCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTggcgggc               >  1:1203377/1‑93 (MQ=255)
                                                                                 cgcATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTa           <  2:5563925/93‑1 (MQ=255)
                                                                                 cgcATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTa           >  2:2197018/1‑93 (MQ=255)
                                                                                     tCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACtt       <  1:4647822/93‑1 (MQ=255)
                                                                                      cACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGTTGGGCTGTATGCTGGCGGGCGGTAACTTc      >  1:493030/1‑93 (MQ=255)
                                                                                      cACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTc      <  1:1283484/93‑1 (MQ=255)
                                                                                       aCCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCt     >  2:3537229/1‑93 (MQ=255)
                                                                                       aCCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCt     >  1:3627916/1‑93 (MQ=255)
                                                                                        ccAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTa    <  2:5935929/93‑1 (MQ=255)
                                                                                            |                                                                                          
TTATGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACC  >  NC_000913/2839560‑2839742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTATGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCT  >  NC_000913/2839559‑2839744
                                                                                             |                                                                                            
ATTATGTTGCATAGCCCGTTCTACGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGC                                                                                              >  SRR4302547.1308073/1‑94 (MQ=60)
   ATGTTGCATAGCCGGTTCTCCGTCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGG                                                                                           <  SRR4302547.4495846/94‑1 (MQ=60)
   ATGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGG                                                                                           <  SRR4302547.2813427/94‑1 (MQ=60)
    TGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGT                                                                                          >  SRR4302547.1062601/1‑94 (MQ=60)
    TGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGT                                                                                          >  SRR4302547.1095078/1‑94 (MQ=60)
     GTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTT                                                                                         <  SRR4302547.4544294/94‑1 (MQ=60)
          ATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAG                                                                                    >  SRR4302547.1223095/1‑94 (MQ=60)
           TAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGT                                                                                   <  SRR4302547.3838695/94‑1 (MQ=60)
           TAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGT                                                                                   <  SRR4302547.2005706/94‑1 (MQ=60)
            AGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTG                                                                                  >  SRR4302547.1653240/1‑94 (MQ=60)
            AGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTG                                                                                  >  SRR4302547.152552/1‑94 (MQ=60)
            AGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTG                                                                                  >  SRR4302547.5641827/1‑94 (MQ=60)
              CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCG                                                                                >  SRR4302547.876972/1‑94 (MQ=60)
              CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCG                                                                                <  SRR4302547.5957522/94‑1 (MQ=60)
              CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGAG                                                                                >  SRR4302547.822687/1‑94 (MQ=60)
               CCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGC                                                                               >  SRR4302547.2558802/1‑94 (MQ=60)
               CCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGC                                                                               >  SRR4302547.6315323/1‑94 (MQ=60)
                CGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCT                                                                              >  SRR4302547.5553678/1‑94 (MQ=60)
                CGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCT                                                                              >  SRR4302547.2578013/1‑94 (MQ=60)
                  TTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAG                                                                            <  SRR4302547.4668822/94‑1 (MQ=60)
                  TTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAG                                                                            <  SRR4302547.6176958/94‑1 (MQ=60)
                     TCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCA                                                                         <  SRR4302547.6677915/94‑1 (MQ=60)
                      CCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCAC                                                                        >  SRR4302547.1610490/1‑94 (MQ=60)
                        GGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCA                                                                      <  SRR4302547.6750548/94‑1 (MQ=60)
                        GGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCA                                                                      >  SRR4302547.3543389/1‑94 (MQ=60)
                          CGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGGCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAA                                                                    >  SRR4302547.6307203/1‑94 (MQ=60)
                          CGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAA                                                                    <  SRR4302547.6493920/94‑1 (MQ=60)
                           GCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAA                                                                   <  SRR4302547.3021093/94‑1 (MQ=60)
                             GGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATC                                                                 <  SRR4302547.2081446/94‑1 (MQ=60)
                             GGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATC                                                                 <  SRR4302547.629073/94‑1 (MQ=60)
                             GGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGGGAAATATCAGGCCGGGCATAACCAGTTGTTTGCCAGGGCGCTGGCACCCAAAATC                                                                 >  SRR4302547.4303995/1‑94 (MQ=57)
                              GGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCG                                                                >  SRR4302547.3888413/1‑94 (MQ=60)
                                TCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCC                                                              >  SRR4302547.2124337/1‑94 (MQ=60)
                                  TGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCGTCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCA                                                            <  SRR4302547.991584/94‑1 (MQ=60)
                                  TGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCA                                                            >  SRR4302547.1756624/1‑94 (MQ=60)
                                   GGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCAT                                                           >  SRR4302547.4725327/1‑94 (MQ=60)
                                   GGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCAT                                                           <  SRR4302547.3188973/94‑1 (MQ=60)
                                    GTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATG                                                          <  SRR4302547.3266755/94‑1 (MQ=60)
                                      GTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAG                                                        <  SRR4302547.5299501/94‑1 (MQ=60)
                                      GTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAG                                                        >  SRR4302547.719086/1‑94 (MQ=60)
                                         TGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGAGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTT                                                     <  SRR4302547.5706053/94‑1 (MQ=60)
                                          GAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTA                                                    >  SRR4302547.1382616/1‑94 (MQ=60)
                                           AAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAA                                                   >  SRR4302547.6488201/1‑94 (MQ=60)
                                            AGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAAC                                                  >  SRR4302547.3752874/1‑94 (MQ=60)
                                            AGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAAC                                                  >  SRR4302547.5567388/1‑94 (MQ=60)
                                            AGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAAC                                                  >  SRR4302547.1172490/1‑94 (MQ=60)
                                              AAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCC                                                <  SRR4302547.3424675/94‑1 (MQ=60)
                                              AAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCC                                                >  SRR4302547.492486/1‑94 (MQ=60)
                                              AAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCC                                                >  SRR4302547.2734845/1‑94 (MQ=60)
                                               AGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCG                                               >  SRR4302547.4562708/1‑94 (MQ=60)
                                                 GTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCA                                             <  SRR4302547.5649123/94‑1 (MQ=60)
                                                  TGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTCGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAA                                            >  SRR4302547.1397205/1‑94 (MQ=60)
                                                   GAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAA                                           >  SRR4302547.4616729/1‑94 (MQ=60)
                                                   GAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGGTGGTTGCCAGTGCGCTAGCCACCAACATCGCCCATGAGGTTAACCCGCAAA                                           >  SRR4302547.5853193/1‑94 (MQ=60)
                                                    AAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAA                                          <  SRR4302547.1631768/94‑1 (MQ=60)
                                                    AAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAA                                          >  SRR4302547.3946726/1‑94 (MQ=60)
                                                     AAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAAT                                         <  SRR4302547.1526383/94‑1 (MQ=60)
                                                     AAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTCCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAAT                                         <  SRR4302547.2818179/94‑1 (MQ=60)
                                                     AAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAAT                                         <  SRR4302547.3605317/94‑1 (MQ=60)
                                                     AAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAAT                                         <  SRR4302547.3246727/94‑1 (MQ=60)
                                                      AATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATC                                        <  SRR4302547.1589084/94‑1 (MQ=60)
                                                       ATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCA                                       <  SRR4302547.5429566/94‑1 (MQ=60)
                                                        TCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAG                                      <  SRR4302547.489723/94‑1 (MQ=60)
                                                          AGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGT                                    <  SRR4302547.4042291/94‑1 (MQ=60)
                                                          AGGATCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGT                                    >  SRR4302547.3753714/1‑94 (MQ=60)
                                                           GGATCAGGTGAAATATCAGGCCGCGAATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGTTTAACCCGCAAAATaagtgg                                   >  SRR4302547.580926/1‑88 (MQ=60)
                                                           GGATCAGGCGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTG                                   >  SRR4302547.6443494/1‑94 (MQ=60)
                                                            GATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGG                                  <  SRR4302547.4841462/94‑1 (MQ=60)
                                                            GATCAGGTGAAATAGCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAGTCGCCCATGAGGTTAACCCGCAAAATCAGGTGG                                  <  SRR4302547.3560366/94‑1 (MQ=60)
                                                             tgCAGGTGAAATATCAGGCCCCGCATCACCAGCTGGTTGCAAGTGCCATAGCCACCAAAATCGCCCATGAGGTTAACCTGCATAATCAGGTGGG                                 <  SRR4302547.4144891/92‑1 (MQ=60)
                                                              TCAGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGC                                >  SRR4302547.6150888/1‑94 (MQ=60)
                                                                AGGTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTG                              <  SRR4302547.1977294/94‑1 (MQ=60)
                                                                  GTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTA                            <  SRR4302547.239732/94‑1 (MQ=60)
                                                                  GTGAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTA                            <  SRR4302547.2597339/94‑1 (MQ=60)
                                                                    GAAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATG                          >  SRR4302547.1101969/1‑94 (MQ=60)
                                                                     AAATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGC                         <  SRR4302547.3599647/94‑1 (MQ=60)
                                                                      AATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCT                        <  SRR4302547.2236625/94‑1 (MQ=60)
                                                                       ATATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTG                       <  SRR4302547.6368693/94‑1 (MQ=60)
                                                                        TATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGG                      >  SRR4302547.3317396/1‑94 (MQ=60)
                                                                        NNNCANGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTANCCNCCAAAATCGCCNNTGNGGNTAACCCGCAAAATCAGGTGGGCTGTATGCTCG                      >  SRR4302547.6219688/1‑94 (MQ=60)
                                                                         ATCAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGC                     <  SRR4302547.3757043/94‑1 (MQ=60)
                                                                          TCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCG                    >  SRR4302547.1592351/1‑94 (MQ=60)
                                                                          ACAGGCCGCGCATCACCAGTTGGTTGCCGGTGCGCTAGCCACCAAAACCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCG                    <  SRR4302547.2099340/94‑1 (MQ=60)
                                                                           CAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGG                   >  SRR4302547.257906/1‑94 (MQ=60)
                                                                           CAGGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGG                   >  SRR4302547.3077320/1‑94 (MQ=60)
                                                                             GGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGC                 >  SRR4302547.1227671/1‑94 (MQ=60)
                                                                             GGCCGCGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGC                 <  SRR4302547.5688492/94‑1 (MQ=60)
                                                                                  CGCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAA            <  SRR4302547.2140668/94‑1 (MQ=60)
                                                                                  CGCATCACCAGTTGGTTCCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGCGCTGTATGCTGGCGGGCGGTAA            >  SRR4302547.2409671/1‑94 (MQ=60)
                                                                                   GCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAAC           >  SRR4302547.6352478/1‑94 (MQ=60)
                                                                                   GCATCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAAC           <  SRR4302547.1134206/94‑1 (MQ=60)
                                                                                      TCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGTTGGGCTGTATGCTGGCGGGCGGTAACTTC        >  SRR4302547.502030/1‑94 (MQ=60)
                                                                                      TCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTC        <  SRR4302547.1506331/94‑1 (MQ=60)
                                                                                      TCACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTC        <  SRR4302547.4744102/94‑1 (MQ=60)
                                                                                       CACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCT       <  SRR4302547.1476123/94‑1 (MQ=60)
                                                                                       CACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCT       <  SRR4302547.1309348/94‑1 (MQ=60)
                                                                                       CACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGGAACTTCT       >  SRR4302547.6523249/1‑94 (MQ=60)
                                                                                       CACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCACAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCT       >  SRR4302547.3301279/1‑94 (MQ=60)
                                                                                       CACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCT       >  SRR4302547.3701913/1‑94 (MQ=60)
                                                                                        ACCAGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTA      >  SRR4302547.2262177/1‑94 (MQ=60)
                                                                                           AGTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCC   >  SRR4302547.1566930/1‑94 (MQ=60)
                                                                                            GTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCT  <  SRR4302547.322164/94‑1 (MQ=60)
                                                                                            GTTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCT  >  SRR4302547.1891449/1‑94 (MQ=60)
                                                                                             |                                                                                            
ATTATGTTGCATAGCCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCT  >  NC_000913/2839559‑2839744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑