Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,125,033 G→A A335V (GCC→GTC)  glcD ← glycolate oxidase subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,0330GA84.7% 578.3 / 70.4 215A335V (GCC→GTC) glcDglycolate oxidase subunit D
Reads supporting (aligned to +/- strand):  ref base G (15/18);  new base A (92/90);  total (107/108)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 227 total aligned reads displayed.

GCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  NC_000913/3124942‑3125125
                                                                                           |                                                                                            
gCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGc                                                                                             >  1:3182430/1‑93 (MQ=255)
 ccAGGCAGGGCGCGACGCGGGCTGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAGCGAcc                                                                                            >  1:3336298/1‑93 (MQ=255)
 ccAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCgaccgacc                                                                                            <  1:4965528/93‑1 (MQ=255)
 ccAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCgaccgacc                                                                                            <  2:6229166/93‑1 (MQ=255)
 ccAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGcc                                                                                            >  1:5853952/1‑93 (MQ=255)
  caggcaggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGAccc                                                                                           >  1:418794/1‑93 (MQ=255)
  caggcaggACGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGAccc                                                                                           >  1:6461719/1‑93 (MQ=255)
   aggcaggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCa                                                                                          <  1:3682932/93‑1 (MQ=255)
    ggcaggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAg                                                                                         >  1:1973895/1‑93 (MQ=255)
      caggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGaa                                                                                       >  1:2616060/1‑93 (MQ=255)
       aggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGaaa                                                                                      <  2:3074488/93‑1 (MQ=255)
       aggGCGCGACGCGGGATGGTGCCATACATGCAGTAGTAATACGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGaaa                                                                                      >  2:5821572/1‑93 (MQ=255)
        gggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAAc                                                                                     <  1:2152762/93‑1 (MQ=255)
        gggCGCGACGCAGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAAc                                                                                     <  2:4548346/93‑1 (MQ=255)
         ggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACg                                                                                    <  2:2142415/93‑1 (MQ=255)
          gcgcGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGt                                                                                   <  1:5096860/93‑1 (MQ=255)
          gcgcGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGt                                                                                   >  1:1140413/1‑93 (MQ=255)
             cgACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAcg                                                                                <  1:181191/93‑1 (MQ=255)
             cgACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAcg                                                                                <  2:5337884/93‑1 (MQ=255)
             cgACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAcg                                                                                >  1:2350601/1‑93 (MQ=255)
              gACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAcgc                                                                               <  2:5602452/93‑1 (MQ=255)
               aCGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAcgcg                                                                              >  1:1581215/1‑93 (MQ=255)
                  cgGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGctc                                                                           <  2:4637004/93‑1 (MQ=255)
                  cgGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGctc                                                                           <  2:3233082/93‑1 (MQ=255)
                   gggATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGctct                                                                          <  2:2299346/93‑1 (MQ=255)
                   gggATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGctct                                                                          >  1:5499936/1‑93 (MQ=255)
                    ggATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGctctc                                                                         >  1:2245742/1‑92 (MQ=255)
                    ggATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTg                                                                         >  1:4904987/1‑93 (MQ=255)
                      aTGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCt                                                                       <  1:4136331/93‑1 (MQ=255)
                       tGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCtt                                                                      <  1:1486660/93‑1 (MQ=255)
                       tGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCtt                                                                      <  1:4769504/93‑1 (MQ=255)
                       tGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCtt                                                                      >  2:1738974/1‑93 (MQ=255)
                         gTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCg                                                                    <  2:4475542/93‑1 (MQ=255)
                         gTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCg                                                                    >  2:5160878/1‑93 (MQ=255)
                           gCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTc                                                                  >  2:886110/1‑93 (MQ=255)
                             catccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCt                                                                <  1:1047671/93‑1 (MQ=255)
                              atccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCtg                                                               >  1:4554394/1‑93 (MQ=255)
                              atccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCtg                                                               <  2:3824450/93‑1 (MQ=255)
                              atccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCtg                                                               >  2:2493912/1‑93 (MQ=255)
                               tccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCtgt                                                              <  2:354095/93‑1 (MQ=255)
                               tccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCtgt                                                              >  2:1864242/1‑93 (MQ=255)
                                     cAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAga                                                        >  1:6380845/1‑93 (MQ=255)
                                      agtagtAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgac                                                       <  2:3110981/93‑1 (MQ=255)
                                      agtagtAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgac                                                       <  2:408497/93‑1 (MQ=255)
                                      agtagtAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgac                                                       >  1:1397753/1‑93 (MQ=255)
                                         agtaATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgacgga                                                    <  2:1639418/93‑1 (MQ=255)
                                          gtaATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgacggac                                                   >  2:209193/1‑93 (MQ=255)
                                            aaTCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGt                                                 >  2:3594630/1‑93 (MQ=255)
                                             aTCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTc                                                >  1:3386001/1‑93 (MQ=255)
                                              tCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGCCGTCa                                               >  1:1324049/1‑93 (MQ=255)
                                              tCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCa                                               <  2:1288556/93‑1 (MQ=255)
                                              tCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCa                                               <  1:2871699/93‑1 (MQ=255)
                                              tCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCa                                               <  1:5767014/93‑1 (MQ=255)
                                                 ggggAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTc                                            >  2:765086/1‑93 (MQ=255)
                                                  gggAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTcg                                           >  1:3061090/1‑93 (MQ=255)
                                                  gggAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTcg                                           >  2:3885317/1‑93 (MQ=255)
                                                  gggAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTcg                                           <  2:2227209/93‑1 (MQ=255)
                                                    gagaTACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTcgcg                                         >  2:5526317/1‑93 (MQ=255)
                                                    gagaTACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTcgcg                                         >  2:690386/1‑93 (MQ=255)
                                                      gaTACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGcc                                       >  1:4388951/1‑93 (MQ=255)
                                                      gaTACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGcc                                       <  1:2800129/93‑1 (MQ=255)
                                                      gaTACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGcc                                       <  2:64222/93‑1 (MQ=255)
                                                       aTACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGccc                                      <  2:6031981/93‑1 (MQ=255)
                                                        tACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCg                                     >  1:2010709/1‑93 (MQ=255)
                                                           gTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCtt                                  >  1:5962490/1‑93 (MQ=255)
                                                           gTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCtt                                  >  1:264138/1‑93 (MQ=255)
                                                           gTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCtt                                  >  2:608813/1‑93 (MQ=255)
                                                            tCCTACCGCCGGGAACGCATTTTTGCTACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCttt                                 <  2:2022081/93‑1 (MQ=255)
                                                            tCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCttt                                 >  2:2781464/1‑93 (MQ=255)
                                                              cTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTca                               >  1:4280730/1‑93 (MQ=255)
                                                              cTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTca                               <  2:5864518/93‑1 (MQ=255)
                                                              cTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTca                               <  1:4735154/93‑1 (MQ=255)
                                                              cTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTca                               >  1:2465620/1‑93 (MQ=255)
                                                               tACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTcaa                              >  2:3426790/1‑93 (MQ=255)
                                                               tACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTcaa                              >  1:3587576/1‑93 (MQ=255)
                                                                 ccgccgGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTcaaca                            >  2:4188929/1‑93 (MQ=255)
                                                                    ccgGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGa                         >  2:5562479/1‑93 (MQ=255)
                                                                     cgcgAACGCATCTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGAt                        <  1:1242235/90‑1 (MQ=255)
                                                                      gggAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATg                       <  2:4585586/93‑1 (MQ=255)
                                                                      gggAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATg                       >  2:2938319/1‑93 (MQ=255)
                                                                      gggAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATg                       >  2:3014967/1‑93 (MQ=255)
                                                                      gggAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATg                       >  2:4453601/1‑93 (MQ=255)
                                                                       ggAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGt                      <  2:5944694/93‑1 (MQ=255)
                                                                        gAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTc                     <  1:913738/93‑1 (MQ=255)
                                                                         aaCGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCg                    >  1:6535751/1‑93 (MQ=255)
                                                                         aaCGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCg                    <  1:6617087/93‑1 (MQ=255)
                                                                         aaCGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCg                    <  1:3855588/93‑1 (MQ=255)
                                                                          aCGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGt                   <  2:161881/93‑1 (MQ=255)
                                                                             cATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTaa                <  1:6326623/93‑1 (MQ=255)
                                                                              aTTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCATCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAc               >  2:4789364/1‑93 (MQ=255)
                                                                                ttttGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAccc             <  2:806695/93‑1 (MQ=255)
                                                                                ttttGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAccc             <  2:6574523/93‑1 (MQ=255)
                                                                                tgttGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAccc             <  1:1073891/91‑1 (MQ=255)
                                                                                   tGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCt          >  2:1542323/1‑93 (MQ=255)
                                                                                      gaccgaccCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGc       >  1:2272799/1‑93 (MQ=255)
                                                                                      gaccgaccCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGc       <  1:4830894/93‑1 (MQ=255)
                                                                                       aCCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCa      >  2:6375827/1‑93 (MQ=255)
                                                                                        ccGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg     >  1:6075785/1‑93 (MQ=255)
                                                                                        ccGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg     >  1:6509542/1‑93 (MQ=255)
                                                                                           gCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCt  >  2:3937715/1‑93 (MQ=255)
                                                                                           |                                                                                            
GCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  NC_000913/3124942‑3125125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  NC_000913/3124940‑3125125
                                                                                             |                                                                                            
ACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGC                                                                                              >  SRR4302547.6256094/1‑94 (MQ=60)
 CGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGC                                                                                             >  SRR4302547.3245850/1‑94 (MQ=60)
 CGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGAC                                                                                             >  SRR4302547.3130043/1‑94 (MQ=60)
  GCCAGGCAGGGCGCGACGCGGGCTGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAGCGACC                                                                                            >  SRR4302547.3403798/1‑94 (MQ=60)
  GCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCC                                                                                            >  SRR4302547.5975071/1‑94 (MQ=60)
  GCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACC                                                                                            <  SRR4302547.3796099/94‑1 (MQ=60)
   CCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCC                                                                                           <  SRR4302547.5068285/94‑1 (MQ=60)
   CCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCC                                                                                           >  SRR4302547.426557/1‑94 (MQ=60)
   CCAGGCAGGACGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCC                                                                                           >  SRR4302547.6596286/1‑94 (MQ=60)
    CAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCA                                                                                          >  SRR4302547.227040/1‑94 (MQ=60)
    CAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAACGCCGGGAACGCATTTTTGCGACCGACCCA                                                                                          >  SRR4302547.6649043/1‑94 (MQ=60)
     AGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAG                                                                                         >  SRR4302547.2013087/1‑94 (MQ=60)
     AGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAG                                                                                         >  SRR4302547.4964118/1‑94 (MQ=60)
     AGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAG                                                                                         <  SRR4302547.3758111/94‑1 (MQ=60)
     AGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAG                                                                                         <  SRR4302547.4233789/94‑1 (MQ=60)
       GCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAA                                                                                       >  SRR4302547.2668772/1‑94 (MQ=60)
        CAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAA                                                                                      <  SRR4302547.5053792/94‑1 (MQ=60)
         TGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGTGATACGTCCTACCGCCGGGAACGCGTTTTTGCGACCGACCCAGAAAC                                                                                     >  SRR4302547.1491539/1‑94 (MQ=60)
          GGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACG                                                                                    <  SRR4302547.80445/94‑1 (MQ=60)
          GGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACG                                                                                    <  SRR4302547.2195743/94‑1 (MQ=60)
           GGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGT                                                                                   >  SRR4302547.1163442/1‑94 (MQ=60)
           GGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGT                                                                                   <  SRR4302547.6763921/94‑1 (MQ=60)
            GCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTA                                                                                  <  SRR4302547.5201950/94‑1 (MQ=60)
             CGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAC                                                                                 <  SRR4302547.1810175/94‑1 (MQ=60)
             CGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTAC                                                                                 <  SRR4302547.4890207/94‑1 (MQ=60)
              GCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACG                                                                                <  SRR4302547.4927177/94‑1 (MQ=60)
              GCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACG                                                                                >  SRR4302547.2398237/1‑94 (MQ=60)
               CGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGC                                                                               >  SRR4302547.1079232/1‑94 (MQ=60)
               CGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGC                                                                               <  SRR4302547.184594/94‑1 (MQ=60)
                GACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCG                                                                              >  SRR4302547.945420/1‑94 (MQ=60)
                GACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCG                                                                              >  SRR4302547.1612683/1‑94 (MQ=60)
                   GCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTC                                                                           >  SRR4302547.6039708/1‑94 (MQ=60)
                   GCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTC                                                                           >  SRR4302547.1523047/1‑94 (MQ=60)
                   GCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTC                                                                           >  SRR4302547.3863072/1‑94 (MQ=60)
                    GGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCT                                                                          <  SRR4302547.2973330/94‑1 (MQ=60)
                    CGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCT                                                                          >  SRR4302547.5613495/1‑94 (MQ=60)
                     GGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTG                                                                         >  SRR4302547.5006557/1‑94 (MQ=60)
                     GGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTG                                                                         >  SRR4302547.147833/1‑94 (MQ=60)
                     GGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTG                                                                         <  SRR4302547.483685/94‑1 (MQ=60)
                     GGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTC                                                                         >  SRR4302547.2291049/1‑94 (MQ=60)
                      GGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGC                                                                        >  SRR4302547.4441479/1‑94 (MQ=60)
                      GGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGC                                                                        <  SRR4302547.2387793/94‑1 (MQ=60)
                        ATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTT                                                                      <  SRR4302547.4220490/94‑1 (MQ=60)
                        ATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTT                                                                      >  SRR4302547.3339006/1‑94 (MQ=60)
                         TGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTC                                                                     <  SRR4302547.1516341/94‑1 (MQ=60)
                         TGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTC                                                                     <  SRR4302547.4868314/94‑1 (MQ=60)
                          GGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCG                                                                    >  SRR4302547.3674159/1‑94 (MQ=60)
                            TGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTC                                                                  >  SRR4302547.6532874/1‑94 (MQ=60)
                              CCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCT                                                                >  SRR4302547.4027952/1‑94 (MQ=60)
                               CATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTG                                                               <  SRR4302547.1068395/94‑1 (MQ=60)
                               CATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTG                                                               >  SRR4302547.6279727/1‑94 (MQ=60)
                               CATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTG                                                               >  SRR4302547.4648608/1‑94 (MQ=60)
                                ATCTATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGT                                                              <  SRR4302547.6052620/94‑1 (MQ=60)
                                ATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGT                                                              >  SRR4302547.1699966/1‑94 (MQ=60)
                                 TCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTG                                                             <  SRR4302547.244188/94‑1 (MQ=60)
                                 TCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTG                                                             <  SRR4302547.3445161/94‑1 (MQ=60)
                                   CATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGCACGCGCTCTGCTTCGTCCTGTGCC                                                           >  SRR4302547.1108100/1‑94 (MQ=60)
                                     TGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAG                                                         <  SRR4302547.4294128/94‑1 (MQ=60)
                                     TGCAGTAGTAATCCGGGGAGATACCTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAG                                                         <  SRR4302547.4746157/94‑1 (MQ=60)
                                      GCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGA                                                        >  SRR4302547.6514001/1‑94 (MQ=60)
                                       CAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGAC                                                       >  SRR4302547.1425712/1‑94 (MQ=60)
                                        AGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACG                                                      >  SRR4302547.1344765/1‑94 (MQ=60)
                                        AGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACG                                                      <  SRR4302547.3921087/94‑1 (MQ=60)
                                        AGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACG                                                      <  SRR4302547.6464178/94‑1 (MQ=60)
                                        AGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACG                                                      >  SRR4302547.6108327/1‑94 (MQ=60)
                                         GTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGG                                                     <  SRR4302547.2904914/94‑1 (MQ=60)
                                            GTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACG                                                  <  SRR4302547.5158577/94‑1 (MQ=60)
                                            GTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACG                                                  <  SRR4302547.1122864/94‑1 (MQ=60)
                                              AATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTC                                                >  SRR4302547.3454871/1‑94 (MQ=60)
                                               ATCCGGGGATATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCA                                               >  SRR4302547.6586013/1‑94 (MQ=60)
                                               ATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGCCGTCA                                               >  SRR4302547.1350658/1‑94 (MQ=60)
                                                TCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAG                                              <  SRR4302547.2929041/94‑1 (MQ=60)
                                                TCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAG                                              <  SRR4302547.2927044/94‑1 (MQ=60)
                                                TCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAG                                              <  SRR4302547.5886282/94‑1 (MQ=60)
                                                   GGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCG                                           >  SRR4302547.3121979/1‑94 (MQ=60)
                                                       AGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCC                                       >  SRR4302547.4479252/1‑94 (MQ=60)
                                                        GATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCC                                      <  SRR4302547.2856198/94‑1 (MQ=60)
                                                        GATACCTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCC                                      <  SRR4302547.4457899/94‑1 (MQ=60)
                                                         ATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCG                                     >  SRR4302547.2050717/1‑94 (MQ=60)
                                                         ATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCG                                     >  SRR4302547.359965/1‑94 (MQ=60)
                                                         ATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCG                                     >  SRR4302547.5369642/1‑94 (MQ=60)
                                                          TACGTCCTACCGCCGGGAACGCATTTTTGGGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGC                                    >  SRR4302547.570674/1‑94 (MQ=60)
                                                          TACGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGC                                    >  SRR4302547.5205727/1‑94 (MQ=60)
                                                            CGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTT                                  >  SRR4302547.269073/1‑94 (MQ=60)
                                                            CGTCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTT                                  >  SRR4302547.6086138/1‑94 (MQ=60)
                                                              TCCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGTCAGACGGACGTCAGTCGCGCCCGCTTTC                                <  SRR4302547.3416586/94‑1 (MQ=60)
                                                               CCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCA                               >  SRR4302547.2515447/1‑94 (MQ=60)
                                                               CCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCA                               >  SRR4302547.4368657/1‑94 (MQ=60)
                                                               CCTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCA                               <  SRR4302547.1901094/94‑1 (MQ=60)
                                                                CTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCggccgaacgtcggcggggccgcctttcac                              >  SRR4302547.5590329/1‑65 (MQ=60)
                                                                CTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAA                              >  SRR4302547.3660762/1‑94 (MQ=60)
                                                                CTACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAA                              <  SRR4302547.4833297/94‑1 (MQ=60)
                                                                  ACCGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTACCAGACGGACGTCAGTCGCGCCCGCTTTCAACA                            >  SRR4302547.2500893/1‑94 (MQ=60)
                                                                    CGCCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAG                          >  SRR4302547.6630224/1‑94 (MQ=60)
                                                                      CCGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGAT                        >  SRR4302547.2035672/1‑94 (MQ=60)
                                                                       CGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATG                       <  SRR4302547.6720591/94‑1 (MQ=60)
                                                                       CGGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATG                       >  SRR4302547.2304332/1‑94 (MQ=60)
                                                                       CGCGAACGCATCTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATG                       <  SRR4302547.1267310/94‑1 (MQ=60)
                                                                        GGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGT                      >  SRR4302547.2133392/1‑94 (MQ=60)
                                                                        GGGAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGT                      >  SRR4302547.2688418/1‑94 (MQ=60)
                                                                          GAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCG                    >  SRR4302547.6671764/1‑94 (MQ=60)
                                                                          GAACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCG                    <  SRR4302547.931365/94‑1 (MQ=60)
                                                                           AACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGT                   <  SRR4302547.6754810/94‑1 (MQ=60)
                                                                           AACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGT                   <  SRR4302547.3934065/94‑1 (MQ=60)
                                                                           AACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCATCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGT                   >  SRR4302547.1404843/1‑94 (MQ=60)
                                                                            ACGCATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTT                  <  SRR4302547.2285889/94‑1 (MQ=60)
                                                                               CATTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAC               <  SRR4302547.6458186/94‑1 (MQ=60)
                                                                                 TTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCC             <  SRR4302547.1202356/94‑1 (MQ=60)
                                                                                 TTTTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCC             <  SRR4302547.2839887/94‑1 (MQ=60)
                                                                                  TTGTGCGACCGACCCAGAAACGTACGCGCTCGGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCG            <  SRR4302547.1271328/94‑1 (MQ=60)
                                                                                  TGTTGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCG            <  SRR4302547.1095321/94‑1 (MQ=60)
                                                                                     TGCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGAAGACAGTCGCGCACGCCTTCAACAAGCTGTCGTTAACAAGCTC         >  SRR4302547.6696273/1‑94 (MQ=60)
                                                                                      GCGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCG        <  SRR4302547.2651784/94‑1 (MQ=60)
                                                                                       CGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGC       >  SRR4302547.5958591/1‑94 (MQ=60)
                                                                                       CGACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGC       >  SRR4302547.2318766/1‑94 (MQ=60)
                                                                                        GACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCA      <  SRR4302547.4931040/94‑1 (MQ=60)
                                                                                        CACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCA      <  SRR4302547.3206245/94‑1 (MQ=60)
                                                                                         ACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAG     >  SRR4302547.6645073/1‑94 (MQ=60)
                                                                                         ACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAG     <  SRR4302547.4634234/94‑1 (MQ=60)
                                                                                         ACCGACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAG     >  SRR4302547.6201818/1‑94 (MQ=60)
                                                                                            GACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  SRR4302547.5529235/1‑94 (MQ=60)
                                                                                            GACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  SRR4302547.4816402/1‑94 (MQ=60)
                                                                                            GACCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  SRR4302547.3189359/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCT  >  NC_000913/3124940‑3125125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑