Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,157,555	T→C	K536E (AAG→GAG)	ygiRQ ←	Fe‑S oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,555	0	T	C	90.1%	743.0 / 59.6	253	K536E (AAG→GAG)	ygiRQ	Fe‑S oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (11/14); new base C (109/119); total (120/133)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

Only 100 of 264 total aligned reads displayed.

AATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATA  >  NC_000913/3157465‑3157645

aaTAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTGAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTcca                                                                                          >  2:3559951/1‑93 (MQ=255)

aaTAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTcca                                                                                          >  1:1047636/1‑93 (MQ=255)

aaTAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTcca                                                                                          >  1:1703382/1‑93 (MQ=255)

aaTAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTcca                                                                                          <  1:856224/93‑1 (MQ=255)

 aTAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTccat                                                                                         <  2:5633857/93‑1 (MQ=255)

    ggggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCcatcat                                                                                      >  1:1207531/1‑93 (MQ=255)

     gggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCatcatc                                                                                     >  2:6483825/1‑93 (MQ=255)

     gggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCatcatc                                                                                     <  2:5726964/93‑1 (MQ=255)

     gggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCatcatc                                                                                     >  1:504731/1‑93 (MQ=255)

      ggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCt                                                                                    <  1:4381245/93‑1 (MQ=255)

      gaaTCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCt                                                                                    <  1:1637622/91‑1 (MQ=255)

        aTCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTc                                                                                  <  1:2719141/93‑1 (MQ=255)

        aTCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTc                                                                                  >  1:4814365/1‑93 (MQ=255)

            gATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATa                                                                              >  2:437517/1‑93 (MQ=255)

              tACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAAc                                                                            >  1:1802306/1‑93 (MQ=255)

               tgtGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACg                                                                           <  2:4512694/91‑1 (MQ=255)

                  gCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGcc                                                                        >  1:1952145/1‑93 (MQ=255)

                  gCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGcc                                                                        >  2:3531116/1‑93 (MQ=255)

                   ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGccc                                                                       >  1:1996713/1‑93 (MQ=255)

                   ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGccc                                                                       <  2:712419/93‑1 (MQ=255)

                    tctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCct                                                                      <  1:5146757/93‑1 (MQ=255)

                    tctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCct                                                                      <  1:492745/93‑1 (MQ=255)

                    tctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCct                                                                      <  2:5214022/93‑1 (MQ=255)

                     ctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCctt                                                                     <  2:2858864/93‑1 (MQ=255)

                      tttACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCcttc                                                                    >  1:2816746/1‑93 (MQ=255)

                      tttACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCcttc                                                                    <  1:3973914/93‑1 (MQ=255)

                        tACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCcttctt                                                                  <  1:4234694/93‑1 (MQ=255)

                         aCCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCttcttc                                                                 <  2:2878679/93‑1 (MQ=255)

                         aCCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCttcttc                                                                 >  1:1806264/1‑93 (MQ=255)

                          cctgcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCg                                                                <  2:3105863/93‑1 (MQ=255)

                           ctgcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCgg                                                               <  1:4511769/93‑1 (MQ=255)

                           ctgcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCgg                                                               >  1:3579016/1‑93 (MQ=255)

                             gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTa                                                             <  1:2177365/93‑1 (MQ=255)

                             gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTa                                                             >  2:4342338/1‑93 (MQ=255)

                              gctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCCGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAt                                                            <  2:134804/92‑1 (MQ=255)

                              cctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAt                                                            >  2:5405063/1‑93 (MQ=255)

                                tgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGt                                                          >  1:1113855/1‑93 (MQ=255)

                                tgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGt                                                          <  2:3838840/93‑1 (MQ=255)

                                tgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGt                                                          >  1:4645475/1‑93 (MQ=255)

                                 gTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGtt                                                         >  2:61893/1‑93 (MQ=255)

                                  ttttGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTc                                                        <  1:6587162/93‑1 (MQ=255)

                                   tttGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGTCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTcc                                                       <  2:4614039/93‑1 (MQ=255)

                                   tttGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTcc                                                       <  1:2583921/93‑1 (MQ=255)

                                     tGAGTAAGTATCGAACCGCTCTTTAAAGCGGACATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCCTCTTCGGTATGATCCgg                                                     >  1:525184/1‑93 (MQ=255)

                                     tGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgg                                                     >  1:4446551/1‑93 (MQ=255)

                                     tGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgg                                                     <  1:3741711/93‑1 (MQ=255)

                                     tGAGTAAGTATCAAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgg                                                     <  1:4269575/93‑1 (MQ=255)

                                      gAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCggg                                                    <  2:17839/93‑1 (MQ=255)

                                       agtaagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgggg                                                   <  2:452828/93‑1 (MQ=255)

                                       agtaagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgggg                                                   >  1:6450627/1‑93 (MQ=255)

                                        gtaagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGc                                                  >  2:2998085/1‑93 (MQ=255)

                                         taagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCa                                                 >  1:5671361/1‑93 (MQ=255)

                                         taagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCa                                                 >  1:1332412/1‑93 (MQ=255)

                                         taagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCa                                                 <  2:1670670/93‑1 (MQ=255)

                                             taTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCt                                             <  1:5535597/93‑1 (MQ=255)

                                              aTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGATCCGGGGCAATCtt                                            >  1:3035210/1‑93 (MQ=255)

                                               tCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGCTAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTc                                           >  1:3902741/1‑93 (MQ=255)

                                               tCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTc                                           >  2:38772/1‑93 (MQ=255)

                                               tCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTc                                           >  2:4768054/1‑93 (MQ=255)

                                               tCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTc                                           >  2:6088232/1‑93 (MQ=255)

                                                   aCAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGAt                                       >  2:4031993/1‑93 (MQ=255)

                                                     aGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATaa                                     <  2:5288207/93‑1 (MQ=255)

                                                     aGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATaa                                     >  1:1165197/1‑93 (MQ=255)

                                                     aGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATaa                                     >  2:6416787/1‑93 (MQ=255)

                                                      gCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAc                                    <  1:2400169/93‑1 (MQ=255)

                                                      gCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAc                                    <  1:5559650/93‑1 (MQ=255)

                                                       ctctTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAcc                                   <  1:6106645/93‑1 (MQ=255)

                                                       ctctTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAcc                                   >  2:1477137/1‑93 (MQ=255)

                                                       ctctTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAcc                                   <  1:5934470/93‑1 (MQ=255)

                                                          tttAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAccgcc                                >  2:3579235/1‑93 (MQ=255)

                                                          tttAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAccgcc                                >  1:2786504/1‑93 (MQ=255)

                                                           ttAAAGCGGTCATAGCTACCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAccgccg                               <  1:5810244/93‑1 (MQ=255)

                                                            tAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGa                              <  2:4564341/93‑1 (MQ=255)

                                                             aaaGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGAc                             >  1:4133638/1‑93 (MQ=255)

                                                              aaGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACg                            <  2:596863/93‑1 (MQ=255)

                                                              aaGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACg                            >  1:2125311/1‑93 (MQ=255)

                                                              aaGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACg                            <  2:1916580/93‑1 (MQ=255)

                                                              aaGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACg                            <  2:5859833/93‑1 (MQ=255)

                                                               aGCGGTCATAGCTGCCCATGCGCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGt                           <  2:2374269/93‑1 (MQ=255)

                                                                gCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTg                          >  2:5116632/1‑93 (MQ=255)

                                                                 cGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGa                         >  1:985768/1‑93 (MQ=255)

                                                                  ggTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAt                        <  2:6134545/93‑1 (MQ=255)

                                                                    tCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATgg                      <  2:2535825/93‑1 (MQ=255)

                                                                    tCATAGCTGCCCATGCCCGGCTCCATCATCTTCCATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATgg                      >  1:2915091/1‑93 (MQ=255)

                                                                      aTAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGt                    <  1:3136206/93‑1 (MQ=255)

                                                                      aTAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGt                    >  2:4417048/1‑93 (MQ=255)

                                                                         gCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGc                 <  2:2241793/93‑1 (MQ=255)

                                                                          cTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGcc                <  1:410106/93‑1 (MQ=255)

                                                                          cTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGcc                >  2:4786911/1‑93 (MQ=255)

                                                                           tGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCa               >  2:566556/1‑93 (MQ=255)

                                                                             cccATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGt             <  2:3257310/93‑1 (MQ=255)

                                                                               cATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTc           <  1:1446902/93‑1 (MQ=255)

                                                                               cATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGCCGCCAGTTc           >  1:3865681/1‑93 (MQ=255)

                                                                                aTGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCt          >  2:3455652/1‑93 (MQ=255)

                                                                                aTGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCt          >  2:4307751/1‑93 (MQ=255)

                                                                                  gCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCttt        <  2:2500261/93‑1 (MQ=255)

                                                                                  gCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCttt        <  1:6209400/93‑1 (MQ=255)

                                                                                      ggCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGata    <  2:2287719/93‑1 (MQ=255)

                                                                                       gCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGatat   >  1:6044975/1‑93 (MQ=255)

                                                                                        cTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGAtata  <  2:2064370/93‑1 (MQ=255)

AATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATA  >  NC_000913/3157465‑3157645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGC  >  NC_000913/3157462‑3157647

TGAAATAGGGGATCAGATCCTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTT                                                                                              <  SRR4302547.6317856/94‑1 (MQ=60)

 GAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCC                                                                                             <  SRR4302547.1551370/94‑1 (MQ=60)

 GAAATAGCGAATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCC                                                                                             <  SRR4302547.2897942/94‑1 (MQ=60)

  AAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCA                                                                                            >  SRR4302547.1068359/1‑94 (MQ=60)

  AAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCA                                                                                            <  SRR4302547.715258/94‑1 (MQ=60)

  AAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCA                                                                                            >  SRR4302547.1736889/1‑94 (MQ=60)

  AAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCA                                                                                            <  SRR4302547.1714990/94‑1 (MQ=60)

   AATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCAT                                                                                           <  SRR4302547.872583/94‑1 (MQ=60)

   AATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCAT                                                                                           >  SRR4302547.2850196/1‑94 (MQ=60)

      AGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCAT                                                                                        >  SRR4302547.1231905/1‑94 (MQ=60)

       GGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATC                                                                                       >  SRR4302547.513909/1‑94 (MQ=60)

        GGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCT                                                                                      >  SRR4302547.931004/1‑94 (MQ=60)

         GGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTT                                                                                     <  SRR4302547.4471341/94‑1 (MQ=60)

         GAATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTT                                                                                     <  SRR4302547.1669994/94‑1 (MQ=60)

          GATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTC                                                                                    >  SRR4302547.4914140/1‑94 (MQ=60)

          GATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTC                                                                                    <  SRR4302547.1949248/94‑1 (MQ=60)

           ATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCG                                                                                   <  SRR4302547.2773737/94‑1 (MQ=60)

           ATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCG                                                                                   <  SRR4302547.5666988/94‑1 (MQ=60)

            TCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGA                                                                                  >  SRR4302547.6214170/1‑94 (MQ=60)

            TCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGA                                                                                  >  SRR4302547.6609480/1‑94 (MQ=60)

             CAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAGCAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGAT                                                                                 <  SRR4302547.2915242/94‑1 (MQ=60)

             CAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGAT                                                                                 >  SRR4302547.4185956/1‑94 (MQ=60)

              AGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATA                                                                                >  SRR4302547.2766094/1‑94 (MQ=60)

              AGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATA                                                                                >  SRR4302547.729366/1‑94 (MQ=60)

                ATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAAC                                                                              >  SRR4302547.1837946/1‑94 (MQ=60)

                ATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAAC                                                                              >  SRR4302547.1719652/1‑94 (MQ=60)

                 TACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACG                                                                             <  SRR4302547.3605519/94‑1 (MQ=60)

                   CTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGC                                                                           >  SRR4302547.4486179/1‑94 (MQ=60)

                   CTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGC                                                                           >  SRR4302547.4924119/1‑94 (MQ=60)

                   CTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGC                                                                           >  SRR4302547.6297972/1‑94 (MQ=60)

                    TGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCC                                                                          >  SRR4302547.1990866/1‑94 (MQ=60)

                    TGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCC                                                                          <  SRR4302547.2036942/94‑1 (MQ=60)

                     GCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCC                                                                         >  SRR4302547.2036435/1‑94 (MQ=60)

                     GCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCC                                                                         >  SRR4302547.6660499/1‑94 (MQ=60)

                       TCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTT                                                                       <  SRR4302547.501740/94‑1 (MQ=60)

                       TCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTT                                                                       <  SRR4302547.5252758/94‑1 (MQ=60)

                        CTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTC                                                                      >  SRR4302547.2873088/1‑94 (MQ=60)

                        CTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTC                                                                      <  SRR4302547.3966267/94‑1 (MQ=60)

                         TTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCT                                                                     <  SRR4302547.4054726/94‑1 (MQ=60)

                          TTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTT                                                                    >  SRR4302547.2174884/1‑94 (MQ=60)

                           TACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTC                                                                   >  SRR4302547.1841965/1‑94 (MQ=60)

                           TACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTC                                                                   <  SRR4302547.4321270/94‑1 (MQ=60)

                           TACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTC                                                                   <  SRR4302547.1114458/94‑1 (MQ=60)

                            ACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCG                                                                  <  SRR4302547.4493930/94‑1 (MQ=60)

                            ACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCG                                                                  >  SRR4302547.1421066/1‑94 (MQ=60)

                            ACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCG                                                                  <  SRR4302547.1336598/94‑1 (MQ=60)

                             CCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGG                                                                 <  SRR4302547.6531590/94‑1 (MQ=60)

                             CCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGG                                                                 >  SRR4302547.3652019/1‑94 (MQ=60)

                             CCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGG                                                                 >  SRR4302547.4974178/1‑94 (MQ=60)

                              CTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGT                                                                >  SRR4302547.5319063/1‑94 (MQ=60)

                              CTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGT                                                                <  SRR4302547.4604988/94‑1 (MQ=60)

                               TGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTA                                                               <  SRR4302547.1307415/94‑1 (MQ=60)

                                GCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAT                                                              >  SRR4302547.3388451/1‑94 (MQ=60)

                                GCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAT                                                              >  SRR4302547.4412352/1‑94 (MQ=60)

                                GCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAT                                                              <  SRR4302547.2220965/94‑1 (MQ=60)

                                GCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAT                                                              >  SRR4302547.2555995/1‑94 (MQ=60)

                                  CTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGT                                                            >  SRR4302547.1136286/1‑94 (MQ=60)

                                  CTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGT                                                            >  SRR4302547.4741679/1‑94 (MQ=60)

                                   TGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTT                                                           >  SRR4302547.5665384/1‑94 (MQ=60)

                                   TGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTT                                                           <  SRR4302547.1180421/94‑1 (MQ=60)

                                   TGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTT                                                           <  SRR4302547.6300713/94‑1 (MQ=60)

                                    GTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTC                                                          <  SRR4302547.6746667/94‑1 (MQ=60)

                                     TTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCC                                                         <  SRR4302547.6724298/94‑1 (MQ=60)

                                      TTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCG                                                        <  SRR4302547.2636066/94‑1 (MQ=60)

                                       TTGAGTAAGTATCGAACCGCTCTTTAAAGCGGACATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCCTCTTCGGTATGATCCGG                                                       >  SRR4302547.534681/1‑94 (MQ=60)

                                       TTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGG                                                       >  SRR4302547.4538511/1‑94 (MQ=60)

                                        TGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGG                                                      >  SRR4302547.1898031/1‑94 (MQ=60)

                                        TGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGG                                                      <  SRR4302547.3818094/94‑1 (MQ=60)

                                        TGAGTAAGTATCAAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGG                                                      <  SRR4302547.4357192/94‑1 (MQ=60)

                                         GAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGG                                                     <  SRR4302547.5226530/94‑1 (MQ=60)

                                         GAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGG                                                     >  SRR4302547.4353714/1‑94 (MQ=60)

                                         GAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGG                                                     >  SRR4302547.6585014/1‑94 (MQ=60)

                                          AGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGC                                                    <  SRR4302547.1690954/94‑1 (MQ=60)

                                           GTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCA                                                   <  SRR4302547.5702966/94‑1 (MQ=60)

                                           GTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCA                                                   >  SRR4302547.5788465/1‑94 (MQ=60)

                                           GTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCA                                                   >  SRR4302547.2168563/1‑94 (MQ=60)

                                           GTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCA                                                   >  SRR4302547.1359194/1‑94 (MQ=60)

                                           GTAAGTATCAAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTAGGTTCCGGGGCA                                                   <  SRR4302547.4143226/94‑1 (MQ=60)

                                            TAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAA                                                  <  SRR4302547.5975407/94‑1 (MQ=60)

                                                TATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTT                                              <  SRR4302547.5649839/94‑1 (MQ=60)

                                                TATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGATCCGGGGCAATCTT                                              >  SRR4302547.3095587/1‑94 (MQ=60)

                                                 ATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGCTAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTC                                             >  SRR4302547.3982108/1‑94 (MQ=60)

                                                   CGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAG                                           >  SRR4302547.4701150/1‑94 (MQ=60)

                                                   CGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAG                                           >  SRR4302547.5829705/1‑94 (MQ=60)

                                                    GAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGA                                          >  SRR4302547.5373701/1‑94 (MQ=60)

                                                      ACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATA                                        <  SRR4302547.1841770/94‑1 (MQ=60)

                                                       CAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAA                                       >  SRR4302547.1188767/1‑94 (MQ=60)

                                                       CAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGAATGTTCCGTGACAATCTTCAGATAA                                       >  SRR4302547.4588082/1‑94 (MQ=60)

                                                        AGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAC                                      >  SRR4302547.2721959/1‑94 (MQ=60)

                                                        AGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAC                                      >  SRR4302547.2255800/1‑94 (MQ=60)

                                                        AGCTCTTTAAAGCGCTCATAGCTGCCCATCCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTGCCGGGGCAATCTTCAGATAAC                                      <  SRR4302547.1149011/94‑1 (MQ=60)

                                                         GCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACC                                     <  SRR4302547.2448782/94‑1 (MQ=60)

                                                         GCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACC                                     <  SRR4302547.5674326/94‑1 (MQ=60)

                                                          CTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCG                                    <  SRR4302547.5239717/94‑1 (MQ=60)

                                                          CTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCG                                    <  SRR4302547.2035521/94‑1 (MQ=60)

                                                          CTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCG                                    <  SRR4302547.6057521/94‑1 (MQ=60)

                                                          CTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCG                                    <  SRR4302547.6233320/94‑1 (MQ=60)

                                                            CTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCC                                  >  SRR4302547.5099314/1‑94 (MQ=60)

                                                            CTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCC                                  >  SRR4302547.2842323/1‑94 (MQ=60)

                                                              TTAAAGCGGTCATAGCTACCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGA                                <  SRR4302547.5930448/94‑1 (MQ=60)

                                                               TAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGAC                               >  SRR4302547.4217740/1‑94 (MQ=60)

                                                               TAAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGAC                               >  SRR4302547.4081995/1‑94 (MQ=60)

                                                                AAAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACG                              >  SRR4302547.2167662/1‑94 (MQ=60)

                                                                 AAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATCACCACCCACGT                             >  SRR4302547.4025443/1‑94 (MQ=60)

                                                                 AAGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGT                             >  SRR4302547.3891908/1‑94 (MQ=60)

                                                                 AAGCGGTCATAGCTGCCCATGCCCGGCTCCACCATCTTCGATAACAGACCTTCTTCtgtttgttttcgggcgatcttttattaaacccaccccg                             >  SRR4302547.3776974/1‑56 (MQ=42)

                                                                  AGCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTG                            <  SRR4302547.5562101/94‑1 (MQ=60)

                                                                   GCGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGA                           >  SRR4302547.1004949/1‑94 (MQ=60)

                                                                    CGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAT                          <  SRR4302547.3975121/94‑1 (MQ=60)

                                                                    CGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAT                          >  SRR4302547.4879220/1‑94 (MQ=60)

                                                                    CGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAT                          <  SRR4302547.3089868/94‑1 (MQ=60)

                                                                    CGGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAT                          >  SRR4302547.4272163/1‑94 (MQ=60)

                                                                     GGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATG                         >  SRR4302547.1798908/1‑94 (MQ=60)

                                                                     GGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATG                         >  SRR4302547.2476029/1‑94 (MQ=60)

                                                                     GGTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATG                         <  SRR4302547.1225495/94‑1 (MQ=60)

                                                                      GTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGG                        <  SRR4302547.616352/94‑1 (MQ=60)

                                                                      GTCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGG                        <  SRR4302547.5815723/94‑1 (MQ=60)

                                                                      GTCATAGCTGCCCATGCCCGGCTCCATCATCTTCCATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGG                        >  SRR4302547.2973231/1‑94 (MQ=60)

                                                                       TCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGG                       <  SRR4302547.2779188/94‑1 (MQ=60)

                                                                       GCATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGG                       <  SRR4302547.2005918/94‑1 (MQ=60)

                                                                        CATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGT                      <  SRR4302547.179177/94‑1 (MQ=60)

                                                                         ATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTC                     <  SRR4302547.4595467/94‑1 (MQ=60)

                                                                         ATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTC                     <  SRR4302547.3198486/94‑1 (MQ=60)

                                                                         ATAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTC                     <  SRR4302547.3661049/94‑1 (MQ=60)

                                                                          TAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCG                    <  SRR4302547.155760/94‑1 (MQ=60)

                                                                          TAGCTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCG                    <  SRR4302547.4262356/94‑1 (MQ=60)

                                                                             CTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCA                 <  SRR4302547.3190189/94‑1 (MQ=60)

                                                                             CTGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCA                 <  SRR4302547.417706/94‑1 (MQ=60)

                                                                              TGCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAG                <  SRR4302547.5984397/94‑1 (MQ=60)

                                                                               GCCCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGT               >  SRR4302547.4354892/1‑94 (MQ=60)

                                                                                CCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTT              >  SRR4302547.6159702/1‑94 (MQ=60)

                                                                                 CCATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGCCGCCAGTTC             >  SRR4302547.3944351/1‑94 (MQ=60)

                                                                                  CATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCT            >  SRR4302547.4831936/1‑94 (MQ=60)

                                                                                  CATGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCT            <  SRR4302547.1475838/94‑1 (MQ=60)

                                                                                    TGCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTT          <  SRR4302547.3146315/94‑1 (MQ=60)

                                                                                     GCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTG         >  SRR4302547.2972712/1‑94 (MQ=60)

                                                                                     GCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTG         <  SRR4302547.6338340/94‑1 (MQ=60)

                                                                                     GCCCGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGAGTCGCCAGTTCTTTG         <  SRR4302547.1451975/94‑1 (MQ=60)

                                                                                        CGGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATA      <  SRR4302547.389046/94‑1 (MQ=60)

                                                                                         GGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATAT     >  SRR4302547.6170405/1‑94 (MQ=60)

                                                                                         GGCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGCCGCCAGTTCTTTGATAT     >  SRR4302547.2853798/1‑94 (MQ=60)

                                                                                          GCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGGAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATA    >  SRR4302547.2577435/1‑94 (MQ=60)

                                                                                          GCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATA    >  SRR4302547.2309428/1‑94 (MQ=60)

                                                                                          GCTCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATA    >  SRR4302547.4879590/1‑94 (MQ=60)

                                                                                            TCCATCATCTTCGCTAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGC  >  SRR4302547.358735/1‑94 (MQ=60)

                                                                                            TCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGC  <  SRR4302547.1745150/94‑1 (MQ=60)

                                                                                            TCCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGC  >  SRR4302547.3427930/1‑94 (MQ=60)

TGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGC  >  NC_000913/3157462‑3157647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`