Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,097 2 bp→GC coding (824‑825/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,0970CG100.0% 992.1 / NA 267T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (122/145);  total (122/145)
*NC_0009133,221,0980GC100.0% 991.7 / NA 268T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (122/146);  total (122/146)

Only 100 of 273 total aligned reads displayed.

CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA  >  NC_000913/3221010‑3221184
                                                                                       ||                                                                                      
cgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCTCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTg                                                                                    <  1:6276288/93‑1 (MQ=255)
 gCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGc                                                                                   >  1:2531252/1‑93 (MQ=255)
    ggAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAgctttgcttt                                                                                >  2:1427054/1‑93 (MQ=255)
     gAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt                                                                               >  1:5481276/1‑93 (MQ=255)
     gAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt                                                                               <  2:5162861/93‑1 (MQ=255)
       atatACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc                                                                             <  2:3623369/93‑1 (MQ=255)
        tataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACt                                                                            <  2:6501694/93‑1 (MQ=255)
         ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg                                                                           <  2:3712706/93‑1 (MQ=255)
         ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg                                                                           <  2:6465787/93‑1 (MQ=255)
          taCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt                                                                          >  2:4188889/1‑93 (MQ=255)
          taCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt                                                                          >  2:4717199/1‑93 (MQ=255)
            cccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCg                                                                        >  1:1046368/1‑93 (MQ=255)
             ccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGa                                                                       >  1:4793596/1‑93 (MQ=255)
             ccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGa                                                                       <  1:6135140/93‑1 (MQ=255)
             ccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGa                                                                       >  2:5319076/1‑93 (MQ=255)
              cTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGaa                                                                      <  1:5439604/93‑1 (MQ=255)
               tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                     >  2:3337387/1‑93 (MQ=255)
                 tttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAg                                                                   >  1:5802597/1‑93 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGACCAGc                                                                  >  2:5262657/1‑93 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  >  2:1882011/1‑93 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  <  2:323885/93‑1 (MQ=255)
                   tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                 >  1:5641281/1‑93 (MQ=255)
                   tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                 >  2:2645125/1‑93 (MQ=255)
                   tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                 <  2:5931124/93‑1 (MQ=255)
                     aTGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                               <  1:2459286/93‑1 (MQ=255)
                     aTGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                               <  1:5742154/93‑1 (MQ=255)
                       ggCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcag                                                             <  2:2559445/93‑1 (MQ=255)
                       gccGAACGCGTGGTACACAGCAGCGCCATTGATCATTTGGAAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcag                                                             <  1:3542107/91‑1 (MQ=255)
                         cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                           >  2:2276859/1‑93 (MQ=255)
                         cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                           >  1:4353972/1‑93 (MQ=255)
                          gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                          <  1:2112360/93‑1 (MQ=255)
                          gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                          <  2:2367681/93‑1 (MQ=255)
                           aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                         <  1:785325/93‑1 (MQ=255)
                           aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                         >  2:3926959/1‑93 (MQ=255)
                           aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                         >  2:444295/1‑93 (MQ=255)
                            aCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATg                                                        <  2:3197470/93‑1 (MQ=255)
                              gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                      >  1:1975174/1‑93 (MQ=255)
                               cgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                     <  1:305307/93‑1 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   <  2:3216193/93‑1 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   >  2:5741259/1‑93 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   >  1:1086632/1‑93 (MQ=255)
                                  ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                  >  1:4410621/1‑93 (MQ=255)
                                  ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                  <  2:1802991/93‑1 (MQ=255)
                                   gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                 <  1:5070269/93‑1 (MQ=255)
                                      cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                              >  2:5791350/1‑93 (MQ=255)
                                      cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                              <  2:4416428/93‑1 (MQ=255)
                                       acaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                             <  2:5987480/93‑1 (MQ=255)
                                       acaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                             >  2:2006183/1‑93 (MQ=255)
                                         agcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcc                                           >  2:6349632/1‑93 (MQ=255)
                                          gcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATccc                                          >  1:1985418/1‑93 (MQ=255)
                                           cagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcccc                                         <  1:2779346/93‑1 (MQ=255)
                                            agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt                                        >  2:2585635/1‑93 (MQ=255)
                                             gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt                                       >  2:1260470/1‑93 (MQ=255)
                                              cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa                                      <  1:2402494/93‑1 (MQ=255)
                                               gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAt                                     <  1:1550387/93‑1 (MQ=255)
                                                ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATc                                    >  1:2981801/1‑93 (MQ=255)
                                                ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATc                                    >  1:3409034/1‑93 (MQ=255)
                                                ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATc                                    >  1:4297806/1‑93 (MQ=255)
                                                 cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt                                   <  1:5563257/93‑1 (MQ=255)
                                                  aTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt                                  <  2:2127668/93‑1 (MQ=255)
                                                  aTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt                                  >  2:6576911/1‑93 (MQ=255)
                                                    tGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTa                                <  1:6561369/93‑1 (MQ=255)
                                                     taTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                               <  1:2233681/92‑1 (MQ=255)
                                                     gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                               >  1:3264739/1‑93 (MQ=255)
                                                      aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                              >  2:2720199/1‑93 (MQ=255)
                                                       tCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCa                             >  1:3958918/1‑93 (MQ=255)
                                                        cATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                            >  2:6147585/1‑93 (MQ=255)
                                                         aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc                           >  2:4956388/1‑93 (MQ=255)
                                                         aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc                           >  2:3989133/1‑93 (MQ=255)
                                                          tttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                          <  2:4925182/93‑1 (MQ=255)
                                                           ttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg                         <  2:1086632/93‑1 (MQ=255)
                                                           ttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg                         <  1:1323337/93‑1 (MQ=255)
                                                             ggCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGt                       >  2:2319027/1‑93 (MQ=255)
                                                              gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                      <  1:6441/93‑1 (MQ=255)
                                                              gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                      >  2:3476753/1‑93 (MQ=255)
                                                                aaTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt                    >  1:4121086/1‑93 (MQ=255)
                                                                aaTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt                    >  1:1780420/1‑93 (MQ=255)
                                                                aaTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt                    <  2:281394/93‑1 (MQ=255)
                                                                 aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg                   >  2:794820/1‑93 (MQ=255)
                                                                 aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg                   <  1:6596290/93‑1 (MQ=255)
                                                                 aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg                   >  1:4938197/1‑93 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  2:2913324/93‑1 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  1:4643463/93‑1 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  1:2912081/93‑1 (MQ=255)
                                                                   tGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAc                 >  2:40575/1‑93 (MQ=255)
                                                                   tGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAc                 >  2:717384/1‑93 (MQ=255)
                                                                      aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCt              >  2:3035036/1‑93 (MQ=255)
                                                                       aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTg             >  2:5649700/1‑93 (MQ=255)
                                                                         aCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaa           >  1:3887829/1‑93 (MQ=255)
                                                                           tGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg         >  2:1536587/1‑93 (MQ=255)
                                                                           tGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg         >  2:5464669/1‑93 (MQ=255)
                                                                            gACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGa        <  2:5077438/93‑1 (MQ=255)
                                                                            gACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGa        <  1:3371913/93‑1 (MQ=255)
                                                                             aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAc       >  1:1238273/1‑93 (MQ=255)
                                                                             aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAc       <  2:4558679/93‑1 (MQ=255)
                                                                             aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAc       <  1:51439/93‑1 (MQ=255)
                                                                               cAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGc     <  1:4453587/93‑1 (MQ=255)
                                                                                aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGcc    <  1:4581330/93‑1 (MQ=255)
                                                                                aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGcc    >  2:2477103/1‑93 (MQ=255)
                                                                                  cgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCaa  <  1:1506353/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA  >  NC_000913/3221010‑3221184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  >  NC_000913/3221004‑3221185
                                                                                             |                                                                                        
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAG                                                                                          <  SRR4302547.2689957/94‑1 (MQ=60)
     ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                     <  SRR4302547.1973249/94‑1 (MQ=60)
      CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCTCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                    <  SRR4302547.6406677/94‑1 (MQ=60)
      CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                    >  SRR4302547.2582414/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                  >  SRR4302547.5696094/1‑94 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                 <  SRR4302547.4200930/94‑1 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                 <  SRR4302547.5978271/94‑1 (MQ=60)
         TGAAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                 >  SRR4302547.4031814/1‑94 (MQ=60)
          GGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTT                                                                                >  SRR4302547.5594459/1‑94 (MQ=60)
          GGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTT                                                                                <  SRR4302547.2123921/94‑1 (MQ=60)
            AATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCCGCTTTGCTTTTAC                                                                              >  SRR4302547.467708/1‑94 (MQ=60)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                             <  SRR4302547.3212547/94‑1 (MQ=60)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                             <  SRR4302547.2517259/94‑1 (MQ=60)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                             >  SRR4302547.4975348/1‑94 (MQ=60)
              TATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTG                                                                            >  SRR4302547.5907473/1‑94 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                           <  SRR4302547.4614692/94‑1 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                           >  SRR4302547.6715666/1‑94 (MQ=60)
                TTCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                          <  SRR4302547.5487961/94‑1 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTCTTACTGTC                                                                          <  SRR4302547.6443157/94‑1 (MQ=60)
                 ACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                         >  SRR4302547.1067053/1‑94 (MQ=60)
                 ACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                         <  SRR4302547.6284116/94‑1 (MQ=60)
                  CCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGA                                                                        >  SRR4302547.4892928/1‑94 (MQ=60)
                   CCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAA                                                                       <  SRR4302547.6262414/94‑1 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                      <  SRR4302547.5552018/94‑1 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                      <  SRR4302547.3345089/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                     <  SRR4302547.4402672/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                     <  SRR4302547.3033888/94‑1 (MQ=60)
                      GTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAG                                                                    <  SRR4302547.3481191/94‑1 (MQ=60)
                      GTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAG                                                                    >  SRR4302547.5922648/1‑94 (MQ=60)
                        TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                  >  SRR4302547.5757728/1‑94 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                 <  SRR4302547.4528884/94‑1 (MQ=60)
                          GATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                <  SRR4302547.4494876/94‑1 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                               >  SRR4302547.3446848/1‑94 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                               <  SRR4302547.2508980/94‑1 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                               <  SRR4302547.5860813/94‑1 (MQ=60)
                             GCCGAACGCGTGGTACACAGCAGCGCCATTGATCATTTGGAAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGC                                                             <  SRR4302547.3614410/94‑1 (MQ=60)
                              GCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCA                                                            >  SRR4302547.4443422/1‑94 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCCTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCTGCAGCAAT                                                          >  SRR4302547.4519000/1‑94 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                          <  SRR4302547.5844948/94‑1 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                          >  SRR4302547.2597369/1‑94 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                          <  SRR4302547.5093190/94‑1 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                          <  SRR4302547.2154442/94‑1 (MQ=60)
                                 gacggtgtGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                         <  SRR4302547.4982353/86‑1 (MQ=60)
                                 AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                         <  SRR4302547.800179/94‑1 (MQ=60)
                                  ACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGG                                                        >  SRR4302547.343795/1‑94 (MQ=60)
                                   CGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGT                                                       >  SRR4302547.2014400/1‑94 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                     <  SRR4302547.310991/94‑1 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                    >  SRR4302547.1536698/1‑94 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                    >  SRR4302547.1108353/1‑94 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCACTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                    <  SRR4302547.1096611/94‑1 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                   >  SRR4302547.4501543/1‑94 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                   <  SRR4302547.5649118/94‑1 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                   >  SRR4302547.5265141/1‑94 (MQ=60)
                                         GTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAG                                                 <  SRR4302547.5174887/94‑1 (MQ=60)
                                           GCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                               <  SRR4302547.307122/94‑1 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGTCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                              <  SRR4302547.1960817/94‑1 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                              >  SRR4302547.5199303/1‑94 (MQ=60)
                                               AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCC                                           >  SRR4302547.2024844/1‑94 (MQ=60)
                                                 CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                         <  SRR4302547.112683/94‑1 (MQ=60)
                                                 CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                         <  SRR4302547.2835031/94‑1 (MQ=60)
                                                  AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTT                                        <  SRR4302547.2208167/94‑1 (MQ=60)
                                                   GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                       <  SRR4302547.4250508/94‑1 (MQ=60)
                                                   GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                       <  SRR4302547.2773281/94‑1 (MQ=60)
                                                    CGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                      <  SRR4302547.2451153/94‑1 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                     >  SRR4302547.3528269/1‑94 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                     >  SRR4302547.3041145/1‑94 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                     <  SRR4302547.1581254/94‑1 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                     >  SRR4302547.4386176/1‑94 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                     >  SRR4302547.3478423/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                    >  SRR4302547.2308480/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                    >  SRR4302547.1566015/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                    <  SRR4302547.2328078/94‑1 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                    <  SRR4302547.1174572/94‑1 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                   <  SRR4302547.5677993/94‑1 (MQ=60)
                                                         TTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTA                                 <  SRR4302547.1753086/94‑1 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                >  SRR4302547.5864089/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                >  SRR4302547.1850699/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                <  SRR4302547.6698001/94‑1 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                >  SRR4302547.3330050/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATACCCTTATCTTTAC                                >  SRR4302547.4574852/1‑94 (MQ=60)
                                                           TATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACC                               <  SRR4302547.2278705/94‑1 (MQ=60)
                                                            ATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCA                              >  SRR4302547.4167881/1‑94 (MQ=60)
                                                            ATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCA                              >  SRR4302547.1282296/1‑94 (MQ=60)
                                                            ATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCA                              <  SRR4302547.332097/94‑1 (MQ=60)
                                                            ATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCA                              >  SRR4302547.4039443/1‑94 (MQ=60)
                                                             TCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAT                             <  SRR4302547.5820554/94‑1 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                           <  SRR4302547.2478014/94‑1 (MQ=60)
                                                                TTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                          <  SRR4302547.5588267/94‑1 (MQ=60)
                                                                TTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                          <  SRR4302547.2022539/94‑1 (MQ=60)
                                                                 TTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGG                         <  SRR4302547.1349932/94‑1 (MQ=60)
                                                                  TGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                        <  SRR4302547.5663855/94‑1 (MQ=60)
                                                                   GGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTC                       >  SRR4302547.4206214/1‑94 (MQ=60)
                                                                    GCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                      <  SRR4302547.1448358/94‑1 (MQ=60)
                                                                    GCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                      <  SRR4302547.6558/94‑1 (MQ=60)
                                                                    GCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                      >  SRR4302547.6603230/1‑94 (MQ=60)
                                                                     CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAT                     >  SRR4302547.17209/1‑94 (MQ=60)
                                                                     CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAT                     >  SRR4302547.4204832/1‑94 (MQ=60)
                                                                     CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAT                     >  SRR4302547.1815705/1‑94 (MQ=60)
                                                                      AATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                    <  SRR4302547.2965946/94‑1 (MQ=60)
                                                                      AATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                    >  SRR4302547.5111909/1‑94 (MQ=60)
                                                                      AATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                    >  SRR4302547.5040478/1‑94 (MQ=60)
                                                                      AATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                    <  SRR4302547.4966313/94‑1 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                   <  SRR4302547.6733619/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                  <  SRR4302547.2970169/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                  <  SRR4302547.444033/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                  <  SRR4302547.4739634/94‑1 (MQ=60)
                                                                         TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                 >  SRR4302547.2171795/1‑94 (MQ=60)
                                                                         TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                 >  SRR4302547.4814126/1‑94 (MQ=60)
                                                                          GAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGC                >  SRR4302547.2555455/1‑94 (MQ=60)
                                                                            AAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG              >  SRR4302547.2982673/1‑94 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA            >  SRR4302547.64312/1‑94 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA            >  SRR4302547.3966912/1‑94 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA            >  SRR4302547.2111714/1‑94 (MQ=60)
                                                                                CTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACGATCTGGTCATGTCGCTGAAAG          <  SRR4302547.6637247/94‑1 (MQ=60)
                                                                                CTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAG          <  SRR4302547.3720097/94‑1 (MQ=60)
                                                                                CTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAG          >  SRR4302547.4837633/1‑94 (MQ=60)
                                                                                 TGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGA         >  SRR4302547.128525/1‑94 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC        >  SRR4302547.3102549/1‑94 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC        >  SRR4302547.5590444/1‑94 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC        <  SRR4302547.3440400/94‑1 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC        >  SRR4302547.1263269/1‑94 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG       <  SRR4302547.52399/94‑1 (MQ=60)
                                                                                     CAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCC     >  SRR4302547.6328208/1‑94 (MQ=60)
                                                                                     CAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCC     >  SRR4302547.3891492/1‑94 (MQ=60)
                                                                                     CAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCC     <  SRR4302547.4545650/94‑1 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA    <  SRR4302547.4676138/94‑1 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA    >  SRR4302547.710380/1‑94 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA    >  SRR4302547.3197426/1‑94 (MQ=60)
                                                                                       GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA   >  SRR4302547.4572050/1‑94 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  <  SRR4302547.1536381/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  <  SRR4302547.737919/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  >  SRR4302547.832644/1‑94 (MQ=60)
                                                                                             |                                                                                        
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  >  NC_000913/3221004‑3221185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑