Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,879 Δ1 bp coding (1005/1434 nt) ygjI → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,8780G.100.0% 1022.8 / NA 216coding (1004/1434 nt)ygjIputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (104/112);  total (104/112)

Only 100 of 242 total aligned reads displayed.

CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC  >  NC_000913/3224786‑3224965
                                                                                            |                                                                                        
cGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcg                                                                                          <  1:1026048/93‑1 (MQ=255)
cGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcg                                                                                          <  2:6031024/93‑1 (MQ=255)
 gCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgc                                                                                         <  1:2524155/93‑2 (MQ=255)
  cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcg                                                                                        >  2:6319881/1‑91 (MQ=255)
    aCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTTGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgca                                                                                      >  1:4354287/1‑89 (MQ=255)
    aCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgca                                                                                      <  1:4049513/93‑5 (MQ=255)
    aCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgca                                                                                      <  1:5013962/93‑5 (MQ=255)
     cGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAACCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAg                                                                                    <  1:521148/93‑1 (MQ=255)
     cGCAGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAg                                                                                    <  2:6609683/93‑1 (MQ=255)
      gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGc                                                                                   <  2:283426/93‑1 (MQ=255)
      gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGc                                                                                   <  1:5495203/93‑1 (MQ=255)
      gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGc                                                                                   <  2:4189385/93‑1 (MQ=255)
       ccGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCg                                                                                  >  2:2070843/1‑93 (MQ=255)
       ccGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCg                                                                                  <  2:3248736/93‑1 (MQ=255)
       ccGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCg                                                                                  >  1:4960159/1‑93 (MQ=255)
       ccGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCg                                                                                  <  2:4325446/93‑1 (MQ=255)
        cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGt                                                                                 >  2:6110576/1‑93 (MQ=255)
        cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGt                                                                                 >  1:6256995/1‑93 (MQ=255)
        cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGt                                                                                 >  1:3093279/1‑93 (MQ=255)
         ggTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTg                                                                                <  2:2377099/93‑1 (MQ=255)
          gTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTgg                                                                               >  1:4637015/1‑93 (MQ=255)
            gAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGAt                                                                             <  1:733355/93‑1 (MQ=255)
                aTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAg                                                                         <  1:6607222/93‑1 (MQ=255)
                  tttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGtt                                                                       <  1:3856450/93‑1 (MQ=255)
                  tttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGtt                                                                       <  1:4180141/93‑1 (MQ=255)
                    tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTcc                                                                     <  2:6494134/93‑1 (MQ=255)
                    tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTcc                                                                     <  1:5125135/93‑1 (MQ=255)
                    tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTcc                                                                     <  1:2937124/93‑1 (MQ=255)
                    tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTcc                                                                     >  1:5912703/1‑93 (MQ=255)
                        ctcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAt                                                                 <  1:679031/93‑1 (MQ=255)
                        ctcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAt                                                                 <  2:2512913/93‑1 (MQ=255)
                         tcCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATc                                                                <  1:2764478/93‑1 (MQ=255)
                           cGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGt                                                              >  1:781903/1‑93 (MQ=255)
                           cGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGt                                                              >  1:4087112/1‑93 (MQ=255)
                           cGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGt                                                              <  2:1250791/93‑1 (MQ=255)
                            gAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTc                                                             <  2:5462662/93‑1 (MQ=255)
                             aaaTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCa                                                            >  2:4900158/1‑93 (MQ=255)
                              aaTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAt                                                           >  2:6223120/1‑93 (MQ=255)
                               aTCCCGGAAGGCATCTTTGGTAAGAAACCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATc                                                          <  2:5744116/93‑1 (MQ=255)
                               aTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATc                                                          <  2:2951019/93‑1 (MQ=255)
                               aTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATc                                                          >  2:940748/1‑93 (MQ=255)
                                tCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATcc                                                         <  2:3050272/93‑1 (MQ=255)
                                tCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATcc                                                         <  1:3394442/93‑1 (MQ=255)
                                  ccGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGGTCCTGATCGTCAtacag                                                       >  1:5409184/1‑89 (MQ=255)
                                     gAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtg                                                    >  1:6560674/1‑93 (MQ=255)
                                      aaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtga                                                   >  2:1496935/1‑93 (MQ=255)
                                      aaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtga                                                   <  1:6084675/93‑1 (MQ=255)
                                      aaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtga                                                   <  1:2587530/93‑1 (MQ=255)
                                      aaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtga                                                   <  1:1762806/93‑1 (MQ=255)
                                        ggCATCTTTGGTATGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgatg                                                 >  1:6384542/1‑93 (MQ=255)
                                          cATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTgatgat                                               <  2:118132/93‑1 (MQ=255)
                                            tCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTa                                             >  2:666018/1‑93 (MQ=255)
                                            tCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTa                                             <  1:4524247/93‑1 (MQ=255)
                                             cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAt                                            <  1:38183/93‑1 (MQ=255)
                                               ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                         <  2:5212501/93‑1 (MQ=255)
                                                tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                        <  2:5135375/93‑2 (MQ=255)
                                                tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                        <  2:3659333/93‑2 (MQ=255)
                                                  gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                      <  2:5534993/93‑4 (MQ=255)
                                                   tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                     <  2:2447998/93‑5 (MQ=255)
                                                     aGAAAACCGTCGAACTGAACGAAAACGGCGCTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                    >  2:142579/1‑93 (MQ=255)
                                                       aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                  <  1:2395865/93‑1 (MQ=255)
                                                       aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                  <  2:1654437/93‑1 (MQ=255)
                                                        aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                 <  1:2537212/93‑1 (MQ=255)
                                                         aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCgg                                >  2:4416623/1‑93 (MQ=255)
                                                          aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGt                               >  2:1754447/1‑93 (MQ=255)
                                                              tctaaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                           <  1:3233398/90‑1 (MQ=255)
                                                              tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                           >  2:3444616/1‑93 (MQ=255)
                                                               cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                          >  2:3961245/1‑93 (MQ=255)
                                                               cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                          <  2:1994048/93‑1 (MQ=255)
                                                                gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                         <  2:728812/93‑1 (MQ=255)
                                                                gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                         >  2:264082/1‑93 (MQ=255)
                                                                gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                         <  1:3010974/93‑1 (MQ=255)
                                                                 aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                        <  2:2285205/93‑1 (MQ=255)
                                                                 aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                        <  2:4838841/93‑1 (MQ=255)
                                                                  aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                       >  1:5691425/1‑93 (MQ=255)
                                                                     gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCgg                    >  1:1661179/1‑92 (MQ=255)
                                                                         gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAg                >  2:2201153/1‑93 (MQ=255)
                                                                         gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAg                >  2:4612288/1‑93 (MQ=255)
                                                                          aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCGCATACCGTGCAgg               >  1:5956509/1‑93 (MQ=255)
                                                                          aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg               >  1:5287511/1‑93 (MQ=255)
                                                                              cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt           <  2:3387901/93‑1 (MQ=255)
                                                                              cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt           <  1:5627583/93‑1 (MQ=255)
                                                                               ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg          >  1:5714380/1‑93 (MQ=255)
                                                                                gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga         >  2:854127/1‑93 (MQ=255)
                                                                                gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga         <  1:1506913/93‑1 (MQ=255)
                                                                                gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga         <  1:6320556/93‑1 (MQ=255)
                                                                                gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGCTCCAATACCGTGCAGGATCtga         >  1:2937121/1‑93 (MQ=255)
                                                                                  gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg       <  2:3719526/93‑1 (MQ=255)
                                                                                  gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg       >  2:3580137/1‑93 (MQ=255)
                                                                                  gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg       >  2:1148047/1‑93 (MQ=255)
                                                                                  gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg       >  1:431016/1‑93 (MQ=255)
                                                                                   ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCGTGCAGAATCtgatga      >  1:3528073/1‑93 (MQ=255)
                                                                                   ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga      >  2:2144594/1‑93 (MQ=255)
                                                                                    tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa     <  2:6411217/93‑1 (MQ=255)
                                                                                    tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa     >  1:4468421/1‑93 (MQ=255)
                                                                                     ccGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt    <  1:477972/93‑1 (MQ=255)
                                                                                      cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa   <  1:1820376/93‑1 (MQ=255)
                                                                                       ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc  <  2:3176428/93‑1 (MQ=255)
                                                                                       ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc  <  2:3125131/93‑1 (MQ=255)
                                                                                       ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc  <  1:3867211/93‑1 (MQ=255)
                                                                                            |                                                                                        
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC  >  NC_000913/3224786‑3224965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  >  NC_000913/3224786‑3224970
                                                                                             |                                                                                            
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGC                                                                                              <  SRR4302547.1046191/94‑1 (MQ=60)
   AACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTTGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCA                                                                                          >  SRR4302547.4443739/1‑94 (MQ=60)
    ACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAG                                                                                         <  SRR4302547.5117602/94‑1 (MQ=60)
    ACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAG                                                                                         <  SRR4302547.4131755/94‑1 (MQ=60)
    ACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAG                                                                                         <  SRR4302547.5153133/94‑1 (MQ=60)
     CGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAACCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGC                                                                                        <  SRR4302547.530596/94‑1 (MQ=60)
      GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCG                                                                                       >  SRR4302547.5062800/1‑94 (MQ=60)
      GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCG                                                                                       >  SRR4302547.5484566/1‑94 (MQ=60)
      GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCG                                                                                       <  SRR4302547.5608662/94‑1 (MQ=60)
       CCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGT                                                                                      >  SRR4302547.6386955/1‑94 (MQ=60)
       CCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGT                                                                                      >  SRR4302547.3154600/1‑94 (MQ=60)
       CCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGT                                                                                      >  SRR4302547.2635230/1‑94 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     >  SRR4302547.6393167/1‑94 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     >  SRR4302547.2384341/1‑94 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     <  SRR4302547.3333446/94‑1 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     >  SRR4302547.4977714/1‑94 (MQ=60)
         GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGG                                                                                    >  SRR4302547.5831434/1‑94 (MQ=60)
         GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGG                                                                                    <  SRR4302547.3603739/94‑1 (MQ=60)
         GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGG                                                                                    <  SRR4302547.4233051/94‑1 (MQ=60)
         GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGG                                                                                    >  SRR4302547.4733065/1‑94 (MQ=60)
         GGTGAAAATTTTCTATTCCGAAATCTCGGAAGGCATCTTTGGTAAGAAAAACGTCGAACTGACCGAAAACGGCGTCCCGGCGC‑GCGCAGCGTGG                                                                                    <  SRR4302547.5742749/94‑1 (MQ=60)
            GAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATC                                                                                 <  SRR4302547.747052/94‑1 (MQ=60)
               AATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAG                                                                              >  SRR4302547.4557862/1‑94 (MQ=60)
                ATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGT                                                                             <  SRR4302547.6744763/94‑1 (MQ=60)
                  TTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTC                                                                           <  SRR4302547.3934941/94‑1 (MQ=60)
                  TTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTC                                                                           <  SRR4302547.4265328/94‑1 (MQ=60)
                   TTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCC                                                                          >  SRR4302547.6035177/1‑94 (MQ=60)
                    TCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCT                                                                         <  SRR4302547.5230735/94‑1 (MQ=60)
                    TCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCT                                                                         <  SRR4302547.2995653/94‑1 (MQ=60)
                    TCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCT                                                                         <  SRR4302547.2995652/94‑1 (MQ=60)
                     CTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTG                                                                        >  SRR4302547.2726106/1‑94 (MQ=60)
                        CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                     <  SRR4302547.691547/94‑1 (MQ=60)
                        CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                     >  SRR4302547.5633253/1‑94 (MQ=60)
                         TCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCG                                                                    <  SRR4302547.2819878/94‑1 (MQ=60)
                         TCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCG                                                                    >  SRR4302547.5172943/1‑94 (MQ=60)
                          CCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGT                                                                   >  SRR4302547.796689/1‑94 (MQ=60)
                          CCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGT                                                                   >  SRR4302547.4170200/1‑94 (MQ=60)
                          CCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGT                                                                   <  SRR4302547.6673711/94‑1 (MQ=60)
                             AAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGAGC‑GCGCAGCGTGGATCCAGGTCCTGATCGTCAT                                                                >  SRR4302547.3257045/1‑94 (MQ=60)
                              AATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATC                                                               >  SRR4302547.6050895/1‑94 (MQ=60)
                              AATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATA                                                               <  SRR4302547.1847141/94‑1 (MQ=60)
                               ATCCCGGAAGGCATCTTTGCTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCC                                                              >  SRR4302547.719220/1‑94 (MQ=60)
                                TCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCC                                                             <  SRR4302547.3463510/94‑1 (MQ=60)
                                TCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCC                                                             <  SRR4302547.6306829/94‑1 (MQ=60)
                                 CCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGGTCCTGATCGTCATacag                                                            >  SRR4302547.5521023/1‑90 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         >  SRR4302547.839704/1‑94 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         >  SRR4302547.6697288/1‑94 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         >  SRR4302547.5767292/1‑94 (MQ=60)
                                     GAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGA                                                        <  SRR4302547.751407/94‑1 (MQ=60)
                                      AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                       <  SRR4302547.6210902/94‑1 (MQ=60)
                                      AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                       <  SRR4302547.1797769/94‑1 (MQ=60)
                                      AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                       <  SRR4302547.2639739/94‑1 (MQ=60)
                                      AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                       >  SRR4302547.357332/1‑94 (MQ=60)
                                      AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGAT                                                       <  SRR4302547.4426252/94‑1 (MQ=60)
                                       AGGCATCTTTGGTATGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATG                                                      >  SRR4302547.6517771/1‑94 (MQ=60)
                                        GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGA                                                     <  SRR4302547.5537255/94‑1 (MQ=60)
                                        GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGA                                                     >  SRR4302547.4699783/1‑94 (MQ=60)
                                         GCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAT                                                    <  SRR4302547.1375166/94‑1 (MQ=60)
                                          CATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATT                                                   >  SRR4302547.3914120/1‑94 (MQ=60)
                                          CATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATT                                                   <  SRR4302547.2999533/94‑1 (MQ=60)
                                           ATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA                                                  >  SRR4302547.6729229/1‑94 (MQ=60)
                                            TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT                                                 >  SRR4302547.5313231/1‑94 (MQ=60)
                                            TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT                                                 <  SRR4302547.4617738/94‑1 (MQ=60)
                                             CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C                                               <  SRR4302547.1714157/94‑1 (MQ=60)
                                             CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C                                               >  SRR4302547.5916928/1‑94 (MQ=60)
                                             CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C                                               <  SRR4302547.6730595/94‑1 (MQ=60)
                                             CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C                                               <  SRR4302547.38898/94‑1 (MQ=60)
                                               TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCC                                             <  SRR4302547.5110882/94‑1 (MQ=60)
                                                TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                            <  SRR4302547.4283799/94‑3 (MQ=60)
                                                TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                            <  SRR4302547.2931288/94‑3 (MQ=60)
                                                 GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                           >  SRR4302547.5696485/1‑91 (MQ=60)
                                                  GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGAT                                           >  SRR4302547.5365535/1‑94 (MQ=60)
                                                   TAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATG                                          >  SRR4302547.1299387/1‑94 (MQ=60)
                                                    AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         >  SRR4302547.425306/1‑94 (MQ=60)
                                                    AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         >  SRR4302547.3034910/1‑94 (MQ=60)
                                                       AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                      >  SRR4302547.1762575/1‑94 (MQ=60)
                                                       AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                      <  SRR4302547.2444395/94‑1 (MQ=60)
                                                        AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGG                                     <  SRR4302547.2588476/94‑1 (MQ=60)
                                                              TCTAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAA                               <  SRR4302547.3297933/94‑1 (MQ=60)
                                                               CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAT                              <  SRR4302547.6183649/94‑1 (MQ=60)
                                                               CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAT                              >  SRR4302547.683129/1‑94 (MQ=60)
                                                               CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAT                              <  SRR4302547.2579202/94‑1 (MQ=60)
                                                                GAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATA                             <  SRR4302547.3070842/94‑1 (MQ=60)
                                                                 AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAC                            <  SRR4302547.4641860/94‑1 (MQ=60)
                                                                 AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAC                            >  SRR4302547.2094992/1‑94 (MQ=60)
                                                                 AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAC                            >  SRR4302547.5808965/1‑94 (MQ=60)
                                                                  ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACC                           <  SRR4302547.33550/94‑1 (MQ=60)
                                                                  ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACC                           >  SRR4302547.725005/1‑94 (MQ=60)
                                                                   CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCG                          >  SRR4302547.3853854/1‑94 (MQ=60)
                                                                    TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCGG                         >  SRR4302547.1693932/1‑94 (MQ=60)
                                                                    TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGT                         >  SRR4302547.6163331/1‑94 (MQ=60)
                                                                     GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTG                        >  SRR4302547.2623570/1‑94 (MQ=60)
                                                                       ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                      <  SRR4302547.2449939/94‑1 (MQ=60)
                                                                       ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                      <  SRR4302547.3985319/94‑1 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     <  SRR4302547.3092403/94‑1 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     >  SRR4302547.4403953/1‑94 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     <  SRR4302547.6498497/94‑1 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGCTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     >  SRR4302547.4804578/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCGCATACCGTGCAGG                    >  SRR4302547.6080018/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    >  SRR4302547.5520954/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    >  SRR4302547.5396586/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    <  SRR4302547.5015745/94‑1 (MQ=60)
                                                                          AAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGA                   >  SRR4302547.1539005/1‑94 (MQ=60)
                                                                             ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                <  SRR4302547.4606819/94‑1 (MQ=60)
                                                                              CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG               >  SRR4302547.5832403/1‑94 (MQ=60)
                                                                              CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG               <  SRR4302547.5743717/94‑1 (MQ=60)
                                                                              CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG               <  SRR4302547.5863053/94‑1 (MQ=60)
                                                                               GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGA              >  SRR4302547.2995649/1‑94 (MQ=60)
                                                                               GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGCTCCAATACCGTGCAGGATCTGA              >  SRR4302547.2995650/1‑94 (MQ=60)
                                                                                GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGAT             <  SRR4302547.1536949/94‑1 (MQ=60)
                                                                                GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGAT             <  SRR4302547.6451977/94‑1 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            >  SRR4302547.438997/1‑94 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            >  SRR4302547.6444588/1‑94 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            >  SRR4302547.813784/1‑94 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCGTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA           <  SRR4302547.405549/94‑1 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCGTGCAGAATCTGATGA           >  SRR4302547.3600112/1‑94 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA           <  SRR4302547.4157308/94‑1 (MQ=60)
                                                                                   TTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA          >  SRR4302547.4560746/1‑94 (MQ=60)
                                                                                     CCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA        <  SRR4302547.486753/94‑1 (MQ=60)
                                                                                      CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC       <  SRR4302547.3944518/94‑1 (MQ=60)
                                                                                      CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC       <  SRR4302547.1856362/94‑1 (MQ=60)
                                                                                       GGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT      <  SRR4302547.3945945/94‑1 (MQ=60)
                                                                                         CGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAT    >  SRR4302547.2879191/1‑94 (MQ=60)
                                                                                         CGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAT    >  SRR4302547.1787858/1‑94 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   <  SRR4302547.250345/92‑1 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   >  SRR4302547.5213907/3‑94 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   <  SRR4302547.865153/92‑1 (MQ=60)
                                                                                            CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  <  SRR4302547.5158984/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  >  NC_000913/3224786‑3224970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑