Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,253,742 Δ1 bp coding (551/552 nt) yhaNM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,253,7410G.99.6% 1087.7 / ‑3.3 232coding (552/552 nt)yhaNMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (108/123);  minor base T (0/1);  total (108/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 250 total aligned reads displayed.

ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253649‑3253829
                                                                                            |                                                                                        
aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCg                                                                                          >  1:100793/1‑93 (MQ=255)
aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCg                                                                                          >  1:4950451/1‑93 (MQ=255)
  aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgtt                                                                                        >  1:1259352/1‑91 (MQ=255)
  aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgtt                                                                                        >  1:5265562/1‑91 (MQ=255)
     gCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTg                                                                                    >  1:462657/1‑93 (MQ=255)
      cacaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTgg                                                                                   <  1:5785624/93‑1 (MQ=255)
      cacaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTgg                                                                                   <  2:4346441/93‑1 (MQ=255)
        caATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTTATTTACGAACTTCAGGATCTCAGCCAGCGGC‑GTTCTGCaa                                                                                 >  2:6311892/1‑93 (MQ=255)
        caATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaa                                                                                 <  2:5188349/93‑1 (MQ=255)
          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               <  1:5724639/93‑1 (MQ=255)
          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               <  2:35630/93‑1 (MQ=255)
          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               >  1:327403/1‑93 (MQ=255)
           tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGc                                                                              <  1:83276/93‑1 (MQ=255)
           tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGc                                                                              <  2:1268193/93‑1 (MQ=255)
           tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGc                                                                              <  1:2479147/93‑1 (MQ=255)
            ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCa                                                                             >  1:4841687/1‑93 (MQ=255)
             tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  1:20952/93‑1 (MQ=255)
              tgcgtCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAcc                                                                           <  2:1245509/89‑1 (MQ=255)
              aGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAcc                                                                           <  1:305800/93‑1 (MQ=255)
              aGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAcc                                                                           <  1:3147841/93‑1 (MQ=255)
              aGCTTCGCGGAATCGAGAATAAAGCGGATCCCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAcc                                                                           <  2:1856958/93‑1 (MQ=255)
               gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGAACCg                                                                          >  2:52523/1‑93 (MQ=255)
               acTTCGCGGAATCGAGAATAAAGCGGATCGCGGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCg                                                                          <  1:3985956/92‑1 (MQ=255)
                  tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       <  1:4004710/93‑1 (MQ=255)
                  tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       <  2:1766817/93‑1 (MQ=255)
                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGc                                                                     >  2:2771392/1‑93 (MQ=255)
                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGCCAGc                                                                     >  1:2573331/1‑93 (MQ=255)
                     cgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCg                                                                    >  1:3688275/1‑93 (MQ=255)
                      ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCgg                                                                   >  1:5659151/1‑93 (MQ=255)
                      ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCgg                                                                   >  1:1720818/1‑93 (MQ=255)
                       gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  <  1:1403906/93‑1 (MQ=255)
                       gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  >  2:1366612/1‑93 (MQ=255)
                       gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  >  2:200446/1‑93 (MQ=255)
                        aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGGCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgag                                                                 >  2:1122886/1‑93 (MQ=255)
                        aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgag                                                                 >  1:1106402/1‑93 (MQ=255)
                         aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTTACTAACTTCAGGATCTCAGCCAGCGAC‑GATCTGGAAAGCACACTCAGCGgaga                                                                >  1:5621336/1‑93 (MQ=255)
                         aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                <  1:2648964/93‑1 (MQ=255)
                         aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                >  2:2650412/1‑93 (MQ=255)
                          tCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAc                                                               >  2:2914260/1‑93 (MQ=255)
                          tCGACAATAAAGCGGATCGCCGCAAACGCGACTTCATTGACGAACTTCAGGATCTCAGCCAGTGT‑TGTTCTGGAAAGCACCGTCAGCGGAGAc                                                               <  1:4326460/93‑1 (MQ=255)
                            gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActc                                                             >  1:2031694/1‑93 (MQ=255)
                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            >  2:4050642/1‑93 (MQ=255)
                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            <  1:6433745/93‑1 (MQ=255)
                              gaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           <  1:957644/93‑1 (MQ=255)
                               aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTg                                                          >  1:3751979/1‑93 (MQ=255)
                                aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGc                                                         >  1:4391478/1‑93 (MQ=255)
                                aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGc                                                         <  1:3035722/93‑1 (MQ=255)
                                 tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCt                                                        <  2:5547210/93‑1 (MQ=255)
                                 tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCt                                                        >  1:2799868/1‑93 (MQ=255)
                                  aaaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                       <  1:1029872/93‑1 (MQ=255)
                                  aaaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                       >  1:5424137/1‑93 (MQ=255)
                                   aaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCg                                                      >  2:2993199/1‑93 (MQ=255)
                                   aaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCg                                                      >  2:3946043/1‑93 (MQ=255)
                                     gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGCTCTCAGCCAGCGTC‑GTTCTGGCAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                                    >  1:2230202/1‑93 (MQ=255)
                                     gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                                    <  2:1442630/93‑1 (MQ=255)
                                         aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctct                                                <  2:526017/93‑1 (MQ=255)
                                          tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTg                                               <  2:1841334/93‑1 (MQ=255)
                                             ccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCca                                            <  2:5959427/93‑1 (MQ=255)
                                              cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcac                                           >  1:3503551/1‑93 (MQ=255)
                                                  aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac                                       >  2:3294654/1‑93 (MQ=255)
                                                    cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCGCTCTGCCACACACGc                                     >  2:6206819/1‑93 (MQ=255)
                                                    cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc                                     >  1:150956/1‑93 (MQ=255)
                                                    cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc                                     >  1:6115142/1‑93 (MQ=255)
                                                     gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGcc                                    <  1:1951002/93‑1 (MQ=255)
                                                      ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCct                                   <  2:1648806/93‑1 (MQ=255)
                                                       gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctg                                  >  2:4320992/1‑93 (MQ=255)
                                                       gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctg                                  <  2:370498/93‑1 (MQ=255)
                                                         cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                <  1:1620762/93‑1 (MQ=255)
                                                         cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                <  2:5089154/93‑1 (MQ=255)
                                                         cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                >  2:5832795/1‑93 (MQ=255)
                                                         cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGAGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                <  2:4573416/93‑1 (MQ=255)
                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                               <  2:498291/93‑1 (MQ=255)
                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                               >  2:675537/1‑93 (MQ=255)
                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                               >  2:866365/1‑93 (MQ=255)
                                                           tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTgg                              >  1:4482481/1‑93 (MQ=255)
                                                           tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTgg                              >  1:2387686/1‑93 (MQ=255)
                                                            cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTggg                             >  2:6075441/1‑93 (MQ=255)
                                                            cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTggg                             >  2:4230801/1‑93 (MQ=255)
                                                              ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTa                           <  1:5120391/93‑1 (MQ=255)
                                                              ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTa                           <  2:661486/93‑1 (MQ=255)
                                                                   gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                      >  2:2508418/1‑93 (MQ=255)
                                                                    aaCTTCAGGATCTCAGCCAGCGTC‑GTTCTCGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGGAAacacc                     >  1:3398584/1‑93 (MQ=255)
                                                                       ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccaca                  >  2:2069842/1‑93 (MQ=255)
                                                                        tCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacac                 <  1:751098/93‑1 (MQ=255)
                                                                        tCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacac                 >  1:6524710/1‑93 (MQ=255)
                                                                           ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAt              <  2:5781386/93‑1 (MQ=255)
                                                                            gATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATc             <  2:54387/93‑1 (MQ=255)
                                                                             aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg            >  2:2349120/1‑93 (MQ=255)
                                                                              tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt           >  2:910911/1‑93 (MQ=255)
                                                                              tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt           >  2:3901263/1‑93 (MQ=255)
                                                                                tcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc         >  2:2597431/1‑93 (MQ=255)
                                                                                tcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc         >  1:6299325/1‑93 (MQ=255)
                                                                                   gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc      >  1:996029/1‑93 (MQ=255)
                                                                                    ccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct     <  2:3060903/93‑1 (MQ=255)
                                                                                    ccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct     >  1:5515131/1‑93 (MQ=255)
                                                                                     cagcGTC‑GTTCTGGAAAGCACCGTCAGCGTAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc    <  2:1032600/93‑1 (MQ=255)
                                                                                     cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc    <  1:5877481/93‑1 (MQ=255)
                                                                                     cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc    <  1:6028860/93‑1 (MQ=255)
                                                                                       gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa  >  1:2884723/1‑93 (MQ=255)
                                                                                       gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa  >  2:3149712/1‑93 (MQ=255)
                                                                                            |                                                                                        
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253649‑3253829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  NC_000913/3253650‑3253835
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TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              >  SRR4302547.5373996/1‑94 (MQ=60)
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              >  SRR4302547.6749051/1‑94 (MQ=60)
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              >  SRR4302547.5962315/1‑94 (MQ=60)
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              >  SRR4302547.1284738/1‑94 (MQ=60)
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              <  SRR4302547.3785105/94‑1 (MQ=60)
  ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCT                                                                                           <  SRR4302547.131364/94‑1 (MQ=60)
   CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTG                                                                                          >  SRR4302547.194783/1‑94 (MQ=60)
   CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTG                                                                                          >  SRR4302547.471200/1‑94 (MQ=60)
    GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGG                                                                                         <  SRR4302547.1256626/94‑1 (MQ=60)
     CACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGA                                                                                        <  SRR4302547.5905300/94‑1 (MQ=60)
      ACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAA                                                                                       <  SRR4302547.253405/94‑1 (MQ=60)
       CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                      <  SRR4302547.6424042/94‑1 (MQ=60)
        AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     >  SRR4302547.333494/1‑94 (MQ=60)
        AATTTAGCTTCGCGGAATCGACAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACAAACGTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     <  SRR4302547.4382471/94‑1 (MQ=60)
         ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    <  SRR4302547.5842898/94‑1 (MQ=60)
         ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    <  SRR4302547.4036413/94‑1 (MQ=60)
          TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCA                                                                                   >  SRR4302547.4942025/1‑94 (MQ=60)
          TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCA                                                                                   <  SRR4302547.2529258/94‑1 (MQ=60)
          TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCA                                                                                   <  SRR4302547.84817/94‑1 (MQ=60)
           TTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAC                                                                                  >  SRR4302547.3717828/1‑94 (MQ=60)
           TTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAC                                                                                  <  SRR4302547.6493390/94‑1 (MQ=60)
            TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                 <  SRR4302547.1628944/94‑1 (MQ=60)
            TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                 <  SRR4302547.1112915/94‑1 (MQ=60)
            TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                 <  SRR4302547.21355/94‑1 (MQ=60)
             AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCG                                                                                <  SRR4302547.3210391/94‑1 (MQ=60)
             AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCG                                                                                <  SRR4302547.311489/94‑1 (MQ=60)
              ACTTCGCGGAATCGAGAATAAAGCGGATCGCGGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGT                                                                               <  SRR4302547.4067001/94‑1 (MQ=60)
               CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTC                                                                              <  SRR4302547.2233032/94‑1 (MQ=60)
                 TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            <  SRR4302547.4086108/94‑1 (MQ=60)
                  CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGC                                                                           <  SRR4302547.777292/94‑1 (MQ=60)
                  CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGCCAGC                                                                           >  SRR4302547.2625282/1‑94 (MQ=60)
                   GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCG                                                                          >  SRR4302547.3763572/1‑94 (MQ=60)
                    CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302547.4897288/1‑94 (MQ=60)
                    CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302547.5775974/1‑94 (MQ=60)
                    CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302547.1754642/1‑94 (MQ=60)
                     GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGA                                                                        <  SRR4302547.2443232/94‑1 (MQ=60)
                      GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       >  SRR4302547.1128671/1‑94 (MQ=60)
                      GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       <  SRR4302547.1431989/94‑1 (MQ=60)
                       AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTTACTAACTTCAGGATCTCAGCCAGCGAC‑GATCTGGAAAGCACACTCAGCGGAGA                                                                      >  SRR4302547.5737347/1‑94 (MQ=60)
                        ATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAC                                                                     <  SRR4302547.3042633/94‑1 (MQ=60)
                        ATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAC                                                                     <  SRR4302547.2702244/94‑1 (MQ=60)
                         TCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACT                                                                    <  SRR4302547.5210897/94‑1 (MQ=60)
                         TCGACAATAAAGCGGATCGCCGCAAACGCGACTTCATTGACGAACTTCAGGATCTCAGCCAGTGT‑TGTTCTGGAAAGCACCGTCAGCGGAGACT                                                                    <  SRR4302547.4415509/94‑1 (MQ=60)
                          CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTC                                                                   >  SRR4302547.2072159/1‑94 (MQ=60)
                            AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 >  SRR4302547.6210287/1‑94 (MQ=60)
                            AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 <  SRR4302547.5891112/94‑1 (MQ=60)
                            AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 >  SRR4302547.6449082/1‑94 (MQ=60)
                            AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 <  SRR4302547.6567840/94‑1 (MQ=60)
                             GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                >  SRR4302547.3828548/1‑94 (MQ=60)
                             GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                <  SRR4302547.3589798/94‑1 (MQ=60)
                             GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                <  SRR4302547.976245/94‑1 (MQ=60)
                              AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                               >  SRR4302547.4481854/1‑94 (MQ=60)
                               ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              <  SRR4302547.3096112/94‑1 (MQ=60)
                               ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              >  SRR4302547.2855933/1‑94 (MQ=60)
                               ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              >  SRR4302547.6118271/1‑94 (MQ=60)
                                TAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTC                                                             >  SRR4302547.5536310/1‑94 (MQ=60)
                                TAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTC                                                             <  SRR4302547.2708518/94‑1 (MQ=60)
                                 AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGGACCGTCAGCGGCGACTCTTGCTCG                                                            >  SRR4302547.4695073/1‑94 (MQ=60)
                                 AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            <  SRR4302547.1050102/94‑1 (MQ=60)
                                  AAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGC                                                           <  SRR4302547.5295614/94‑1 (MQ=60)
                                   AGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGCTCTCAGCCAGCGTC‑GTTCTGGCAAGCACCGTCAGCGGAGACTCTTGCTCGCC                                                          >  SRR4302547.2275154/1‑94 (MQ=60)
                                       GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                      <  SRR4302547.4242114/94‑1 (MQ=60)
                                       GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                      >  SRR4302547.3355079/1‑94 (MQ=60)
                                       GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                      <  SRR4302547.1694895/94‑1 (MQ=60)
                                        ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     >  SRR4302547.3109709/1‑94 (MQ=60)
                                        ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     <  SRR4302547.3161416/94‑1 (MQ=60)
                                        ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     <  SRR4302547.5776393/94‑1 (MQ=60)
                                           caCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  <  SRR4302547.2650231/92‑1 (MQ=60)
                                           GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  <  SRR4302547.6198731/94‑1 (MQ=60)
                                           GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  <  SRR4302547.553272/94‑1 (MQ=60)
                                           GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  >  SRR4302547.1492536/1‑94 (MQ=60)
                                            CCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCAC                                                 >  SRR4302547.5523179/1‑94 (MQ=60)
                                            CCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCAC                                                 >  SRR4302547.3575053/1‑94 (MQ=60)
                                              GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACAC                                               >  SRR4302547.4449983/1‑94 (MQ=60)
                                               CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              <  SRR4302547.1002284/94‑1 (MQ=60)
                                               CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              >  SRR4302547.4059833/1‑94 (MQ=60)
                                               CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              >  SRR4302547.5650006/1‑94 (MQ=60)
                                                AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAC                                             >  SRR4302547.2022420/1‑94 (MQ=60)
                                                AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAC                                             >  SRR4302547.3390110/1‑94 (MQ=60)
                                                 AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCATCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACG                                            <  SRR4302547.2087963/94‑1 (MQ=60)
                                                  ACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGC                                           >  SRR4302547.6241997/1‑94 (MQ=60)
                                                  ACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGC                                           >  SRR4302547.153707/1‑94 (MQ=60)
                                                   CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                          >  SRR4302547.3135966/1‑94 (MQ=60)
                                                   CGCGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                          >  SRR4302547.3537938/1‑94 (MQ=60)
                                                    GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302547.4552980/94‑1 (MQ=60)
                                                    GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302547.1053174/94‑1 (MQ=60)
                                                    GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302547.1989694/94‑1 (MQ=60)
                                                      GACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGC                                       <  SRR4302547.406808/94‑1 (MQ=60)
                                                       ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302547.3324791/1‑94 (MQ=60)
                                                       ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302547.5984807/1‑94 (MQ=60)
                                                       ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302547.5206497/1‑94 (MQ=60)
                                                        CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                     <  SRR4302547.1652836/94‑1 (MQ=60)
                                                         TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                    >  SRR4302547.4575085/1‑94 (MQ=60)
                                                         TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                    >  SRR4302547.4516227/1‑94 (MQ=60)
                                                         TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                    >  SRR4302547.2436057/1‑94 (MQ=60)
                                                          TCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                   <  SRR4302547.6569807/94‑1 (MQ=60)
                                                          TCACTGACGAGCTTCAGCATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCGCGCCCTCTGCCACTCACGCCTGCTGGG                                   <  SRR4302547.6569808/94‑1 (MQ=60)
                                                             TTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAA                                <  SRR4302547.5225887/94‑1 (MQ=60)
                                                              taacaAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               <  SRR4302547.1784994/89‑1 (MQ=60)
                                                              TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               <  SRR4302547.2388455/94‑1 (MQ=60)
                                                              TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               >  SRR4302547.4332317/1‑94 (MQ=60)
                                                              TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               <  SRR4302547.4949627/94‑1 (MQ=60)
                                                               TACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                              <  SRR4302547.259590/94‑1 (MQ=60)
                                                               GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                              >  SRR4302547.2355046/1‑94 (MQ=60)
                                                                ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             <  SRR4302547.5837286/94‑1 (MQ=60)
                                                                 CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                            <  SRR4302547.890837/94‑1 (MQ=60)
                                                                 CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                            >  SRR4302547.5400424/1‑94 (MQ=60)
                                                                 CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                            >  SRR4302547.2099640/1‑94 (MQ=60)
                                                                  GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTCGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGGAAACACC                           >  SRR4302547.3467740/1‑94 (MQ=60)
                                                                     CTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACA                        <  SRR4302547.5649285/94‑1 (MQ=60)
                                                                      TTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                       >  SRR4302547.6660541/1‑94 (MQ=60)
                                                                      TTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                       >  SRR4302547.285884/1‑94 (MQ=60)
                                                                       TCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACC                      <  SRR4302547.765262/94‑1 (MQ=60)
                                                                          TGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                   >  SRR4302547.2741375/1‑94 (MQ=60)
                                                                            ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                 <  SRR4302547.1561757/94‑1 (MQ=60)
                                                                             TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                >  SRR4302547.726480/1‑94 (MQ=60)
                                                                              TTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               <  SRR4302547.1037090/94‑1 (MQ=60)
                                                                              CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACANACGCCTGCTGGGTAAACACCACACCATCGTGC               >  SRR4302547.4847770/1‑94 (MQ=60)
                                                                              CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               <  SRR4302547.1323110/94‑1 (MQ=60)
                                                                              CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               >  SRR4302547.6430268/1‑94 (MQ=60)
                                                                                 AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            >  SRR4302547.883766/1‑94 (MQ=60)
                                                                                 AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            >  SRR4302547.1015436/1‑94 (MQ=60)
                                                                                 AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            >  SRR4302547.2988397/1‑94 (MQ=60)
                                                                                 AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            >  SRR4302547.1969095/1‑94 (MQ=60)
                                                                                  GCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCT           >  SRR4302547.5628982/1‑94 (MQ=60)
                                                                                   CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC          <  SRR4302547.5774236/94‑1 (MQ=60)
                                                                                    CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         <  SRR4302547.5999158/94‑1 (MQ=60)
                                                                                    CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         <  SRR4302547.6153977/94‑1 (MQ=60)
                                                                                     AGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA        >  SRR4302547.1015975/1‑94 (MQ=60)
                                                                                     AGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA        >  SRR4302547.2942298/1‑94 (MQ=60)
                                                                                       CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA      >  SRR4302547.3804395/1‑94 (MQ=60)
                                                                                       CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA      >  SRR4302547.5842797/1‑94 (MQ=60)
                                                                                       CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA      >  SRR4302547.1006576/1‑94 (MQ=60)
                                                                                        GTC‑GTTCTGGAAAGCACCGTCAGTGGTGACTCTTGCTCGCCCTCTGCCACACACGCCGGCTGGGCAAACACCACACCATCGTGCGTCTCGATAT     >  SRR4302547.6784119/1‑94 (MQ=60)
                                                                                        GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT     <  SRR4302547.5959187/94‑1 (MQ=60)
                                                                                        GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT     >  SRR4302547.5777327/1‑94 (MQ=60)
                                                                                        GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT     <  SRR4302547.4086389/94‑1 (MQ=60)
                                                                                          tcGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATG    <  SRR4302547.3998515/92‑1 (MQ=60)
                                                                                            GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  SRR4302547.6652394/1‑94 (MQ=60)
                                                                                            |                                                                                             
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  NC_000913/3253650‑3253835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑