Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,312,970 T→C H229R (CAC→CGC)  infB ← protein chain initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,312,9700TC84.4% 730.7 / 117.3 295H229R (CAC→CGC) infBprotein chain initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (18/28);  new base C (106/143);  total (124/171)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 306 total aligned reads displayed.

CTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCT  >  NC_000913/3312878‑3313062
                                                                                            |                                                                                            
ctgctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGt                                                                                              >  1:993521/1‑93 (MQ=255)
    tttCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATa                                                                                          <  1:791730/93‑1 (MQ=255)
      tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAAt                                                                                        <  2:5118295/93‑1 (MQ=255)
      tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAAt                                                                                        <  1:574354/93‑1 (MQ=255)
      tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAAt                                                                                        <  2:6032957/93‑1 (MQ=255)
      tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAAt                                                                                        >  1:1746175/1‑93 (MQ=255)
       cgcgTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATc                                                                                       <  2:1286391/93‑1 (MQ=255)
          gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCt                                                                                    >  1:6003795/1‑93 (MQ=255)
           ttccGACCACCGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTGCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTg                                                                                   <  1:4245549/90‑1 (MQ=255)
           ttACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTg                                                                                   <  1:2010378/93‑1 (MQ=255)
           ttACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTg                                                                                   >  2:6341111/1‑93 (MQ=255)
              cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGaa                                                                                <  1:1869177/93‑1 (MQ=255)
               gACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAAt                                                                               <  2:5239427/93‑1 (MQ=255)
                aCCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATc                                                                              <  2:3197633/93‑1 (MQ=255)
                 ccacggccacggCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCt                                                                             >  1:4180029/1‑93 (MQ=255)
                   acggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTc                                                                           <  1:2116782/93‑1 (MQ=255)
                   acggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTc                                                                           <  2:6574373/93‑1 (MQ=255)
                   acggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTc                                                                           >  1:3545759/1‑93 (MQ=255)
                    cggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCa                                                                          <  1:1189616/93‑1 (MQ=255)
                      gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGt                                                                        <  2:2509115/93‑1 (MQ=255)
                      gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGt                                                                        >  2:2204789/1‑93 (MQ=255)
                      gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGt                                                                        >  2:1846835/1‑93 (MQ=255)
                      gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGt                                                                        >  2:5308945/1‑93 (MQ=255)
                      gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGt                                                                        >  1:2175876/1‑93 (MQ=255)
                       ccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTc                                                                       >  2:3782122/1‑93 (MQ=255)
                       ccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTc                                                                       <  2:11099/93‑1 (MQ=255)
                        cacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCg                                                                      <  2:5943438/93‑1 (MQ=255)
                           ggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGtt                                                                   <  2:4223392/93‑1 (MQ=255)
                           ggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGtt                                                                   <  2:912807/93‑1 (MQ=255)
                            gccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTc                                                                  <  1:4991924/93‑1 (MQ=255)
                            gccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTc                                                                  <  2:4803903/93‑1 (MQ=255)
                            gccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTc                                                                  <  1:1669175/93‑1 (MQ=255)
                            gccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTc                                                                  >  2:4542883/1‑93 (MQ=255)
                             ccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTcc                                                                 <  1:4803552/93‑1 (MQ=255)
                              cgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCcg                                                                <  2:4158781/93‑1 (MQ=255)
                               gccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCcgc                                                               <  1:5055417/93‑1 (MQ=255)
                               gccTTCGACTCCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCcgc                                                               <  1:2778892/93‑1 (MQ=255)
                                 cTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGt                                                             <  2:207930/93‑1 (MQ=255)
                                 cTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGt                                                             >  1:4623533/1‑93 (MQ=255)
                                 cTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGt                                                             >  1:6230285/1‑93 (MQ=255)
                                 cTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGt                                                             <  1:907449/93‑1 (MQ=255)
                                   tCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTa                                                           <  1:6528578/93‑1 (MQ=255)
                                    cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGGGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTAt                                                          >  2:5046404/1‑93 (MQ=255)
                                    cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGTAATCTTCAGTCGGTTCCGCGTTAt                                                          <  1:1010001/93‑1 (MQ=255)
                                    cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTAt                                                          <  2:2802793/93‑1 (MQ=255)
                                     gACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATc                                                         >  2:3623011/1‑93 (MQ=255)
                                       cTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAg                                                       <  1:5098911/93‑1 (MQ=255)
                                        ttCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGt                                                      >  2:2318780/1‑93 (MQ=255)
                                          cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTcc                                                    <  2:541784/93‑1 (MQ=255)
                                           aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTcta                                                   <  1:6362541/93‑3 (MQ=255)
                                           aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCa                                                   <  2:4463305/93‑1 (MQ=255)
                                             gATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAtt                                                 >  2:1269255/1‑93 (MQ=255)
                                             gATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAtt                                                 <  2:5661955/93‑1 (MQ=255)
                                              aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAttt                                                >  2:4710195/1‑93 (MQ=255)
                                              aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAttt                                                >  1:3227433/1‑93 (MQ=255)
                                              aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAttt                                                <  1:5314316/93‑1 (MQ=255)
                                              aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAttt                                                >  2:5800272/1‑93 (MQ=255)
                                               tCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTg                                               >  2:2843294/1‑93 (MQ=255)
                                                 gCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGtt                                             >  1:431695/1‑93 (MQ=255)
                                                 gCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGtt                                             <  1:2606123/93‑1 (MQ=255)
                                                 gCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGtt                                             >  2:5862875/1‑93 (MQ=255)
                                                   tttCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGtttt                                           >  2:2762405/1‑93 (MQ=255)
                                                   tttCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGtttt                                           <  2:5901307/93‑1 (MQ=255)
                                                    ttCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTttc                                          <  1:612383/93‑1 (MQ=255)
                                                     tCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTttct                                         <  1:3651090/93‑1 (MQ=255)
                                                     tCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTttct                                         <  2:388338/93‑1 (MQ=255)
                                                       gTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTttcttc                                       <  2:1485953/93‑1 (MQ=255)
                                                        tcttctGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTcc                                      >  2:1978553/1‑93 (MQ=255)
                                                          ttctGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGc                                    >  1:6039364/1‑93 (MQ=255)
                                                          ttctGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGc                                    <  1:841975/93‑1 (MQ=255)
                                                           tctGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGcc                                   >  2:3031313/1‑93 (MQ=255)
                                                              gCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATa                                >  1:2935333/1‑93 (MQ=255)
                                                                cTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATacg                              >  1:2368688/1‑93 (MQ=255)
                                                                 tGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATacga                             >  2:2403692/1‑93 (MQ=255)
                                                                    cGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGt                          >  2:3501265/1‑93 (MQ=255)
                                                                      aGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGc                        <  2:3428150/93‑1 (MQ=255)
                                                                       ccATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGCCGTGct                       <  2:1343061/92‑1 (MQ=255)
                                                                        cATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGctt                      <  1:524402/93‑1 (MQ=255)
                                                                         aTGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGcttc                     <  1:5373640/93‑1 (MQ=255)
                                                                          tGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGcttct                    >  1:1622335/1‑93 (MQ=255)
                                                                            ttGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCttcttc                  <  2:6604660/93‑1 (MQ=255)
                                                                             tGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCa                 <  1:3066173/93‑1 (MQ=255)
                                                                             tGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCa                 >  1:38287/1‑93 (MQ=255)
                                                                              gagaAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAg                >  1:683993/1‑93 (MQ=255)
                                                                              gagaAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAg                >  2:5979494/1‑93 (MQ=255)
                                                                               agaAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGc               >  2:4850796/1‑93 (MQ=255)
                                                                                 aaGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCaa             <  1:4951484/93‑1 (MQ=255)
                                                                                  agtagtGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAAc            >  2:1412604/1‑93 (MQ=255)
                                                                                  agtagtGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAAc            <  2:3711214/93‑1 (MQ=255)
                                                                                  agtaggGACGTGATAAGCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTGCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAAc            <  2:688446/93‑1 (MQ=255)
                                                                                     agtGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAAcgcg         >  1:3943273/1‑93 (MQ=255)
                                                                                      gtGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGa        <  2:6475282/93‑1 (MQ=255)
                                                                                      gtGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGa        >  2:833816/1‑93 (MQ=255)
                                                                                      gtGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGa        <  2:1184415/93‑1 (MQ=255)
                                                                                       tGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGAc       <  2:6253715/93‑1 (MQ=255)
                                                                                       tGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGAc       <  2:1763507/93‑1 (MQ=255)
                                                                                       tGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGAc       <  1:5639336/93‑1 (MQ=255)
                                                                                         aCGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGt     <  2:2590237/93‑1 (MQ=255)
                                                                                         aCGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGt     <  1:5597835/93‑1 (MQ=255)
                                                                                         aCGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGt     <  2:161833/93‑1 (MQ=255)
                                                                                            |                                                                                            
CTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCT  >  NC_000913/3312878‑3313062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  >  NC_000913/3312877‑3313060
                                                                                             |                                                                                          
GCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGT                                                                                            >  SRR4302547.1012876/1‑94 (MQ=60)
  TGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGA                                                                                          <  SRR4302547.3578580/94‑1 (MQ=60)
    CTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATA                                                                                        >  SRR4302547.5692750/1‑94 (MQ=60)
    CTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATA                                                                                        <  SRR4302547.6667306/94‑1 (MQ=60)
     TTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGTAGTAGTGACGTGATAA                                                                                       >  SRR4302547.457869/1‑94 (MQ=60)
     TTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAA                                                                                       <  SRR4302547.806713/94‑1 (MQ=60)
      TTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAAT                                                                                      >  SRR4302547.1780610/1‑94 (MQ=60)
       TCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATC                                                                                     <  SRR4302547.6153922/94‑1 (MQ=60)
       TCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATC                                                                                     <  SRR4302547.584662/94‑1 (MQ=60)
         GCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGC                                                                                   <  SRR4302547.6574482/94‑1 (MQ=60)
          CTTTACGACCACGGCTACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTAATAATCGCT                                                                                  <  SRR4302547.4364054/94‑1 (MQ=60)
          CGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCT                                                                                  >  SRR4302547.725855/1‑94 (MQ=60)
          CGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCT                                                                                  >  SRR4302547.3380716/1‑94 (MQ=60)
          CGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCT                                                                                  >  SRR4302547.167471/1‑94 (MQ=60)
          CGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCT                                                                                  >  SRR4302547.6128388/1‑94 (MQ=60)
          CGCTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCTATAATCGCT                                                                                  <  SRR4302547.6023757/94‑1 (MQ=60)
           GTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTG                                                                                 >  SRR4302547.2213255/1‑94 (MQ=60)
            TTCCGACCACCGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTGCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGG                                                                                <  SRR4302547.4332391/94‑1 (MQ=60)
            TTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGG                                                                                <  SRR4302547.2050383/94‑1 (MQ=60)
              ACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAA                                                                              >  SRR4302547.1712829/1‑94 (MQ=60)
               CGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAAT                                                                             <  SRR4302547.1906088/94‑1 (MQ=60)
               CGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAAT                                                                             <  SRR4302547.4886111/94‑1 (MQ=60)
                GACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATC                                                                            >  SRR4302547.3264938/1‑94 (MQ=60)
                GACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATC                                                                            >  SRR4302547.5735400/1‑94 (MQ=60)
                 ACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCT                                                                           >  SRR4302547.2095911/1‑94 (MQ=60)
                 ACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCT                                                                           <  SRR4302547.5774460/94‑1 (MQ=60)
                 ACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCT                                                                           >  SRR4302547.4265214/1‑94 (MQ=60)
                  CCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTT                                                                          <  SRR4302547.1839558/94‑1 (MQ=60)
                   CACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTC                                                                         >  SRR4302547.3731903/1‑94 (MQ=60)
                   CACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTC                                                                         >  SRR4302547.3618118/1‑94 (MQ=60)
                    GCGGCCCCGGCCGCCTTCGACTTCACCATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGCAGTGACGCGATAATCGCTGCAATCTTCA                                                                        <  SRR4302547.4287906/94‑1 (MQ=60)
                    CCGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGGAGTGACGCGATAATCGCTGGAATCTTCA                                                                        <  SRR4302547.506250/94‑1 (MQ=60)
                    ACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCA                                                                        <  SRR4302547.2158967/94‑1 (MQ=60)
                    ACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCA                                                                        >  SRR4302547.2188292/1‑94 (MQ=60)
                     CGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAG                                                                       >  SRR4302547.384704/1‑94 (MQ=60)
                     CGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAG                                                                       <  SRR4302547.1213631/94‑1 (MQ=60)
                     CGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAG                                                                       >  SRR4302547.4434931/1‑94 (MQ=60)
                     CGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAG                                                                       <  SRR4302547.6242181/94‑1 (MQ=60)
                      GGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGT                                                                      >  SRR4302547.2219444/1‑94 (MQ=60)
                      GGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGT                                                                      >  SRR4302547.3913018/1‑94 (MQ=60)
                       GCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTC                                                                     <  SRR4302547.5836987/94‑1 (MQ=60)
                        CCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCG                                                                    <  SRR4302547.5088079/94‑1 (MQ=60)
                        CCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCG                                                                    <  SRR4302547.3662002/94‑1 (MQ=60)
                        CCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGCAGTGACGCGGTAATCGCTGGAATCTTCAGTCG                                                                    >  SRR4302547.5376550/1‑94 (MQ=60)
                         CACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGG                                                                   <  SRR4302547.4006142/94‑1 (MQ=60)
                         CACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGG                                                                   <  SRR4302547.5893312/94‑1 (MQ=60)
                          ACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGT                                                                  <  SRR4302547.5509974/94‑1 (MQ=60)
                             GCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCC                                                               <  SRR4302547.5095102/94‑1 (MQ=60)
                             GCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCC                                                               <  SRR4302547.839593/94‑1 (MQ=60)
                             GCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCC                                                               <  SRR4302547.1702068/94‑1 (MQ=60)
                              CCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCG                                                              <  SRR4302547.4903084/94‑1 (MQ=60)
                                GCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCG                                                            <  SRR4302547.2290996/94‑1 (MQ=60)
                                GCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCG                                                            <  SRR4302547.5159768/94‑1 (MQ=60)
                                GCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCG                                                            <  SRR4302547.3062195/94‑1 (MQ=60)
                                GCCTTCGACTCCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCG                                                            <  SRR4302547.2834570/94‑1 (MQ=60)
                                 CCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGT                                                           >  SRR4302547.6359695/1‑94 (MQ=60)
                                 CCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGT                                                           <  SRR4302547.5003271/94‑1 (MQ=60)
                                 CCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGT                                                           >  SRR4302547.4719286/1‑94 (MQ=60)
                                  CTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTT                                                          <  SRR4302547.924940/94‑1 (MQ=60)
                                    TCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTAT                                                        <  SRR4302547.6664469/94‑1 (MQ=60)
                                     CGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGTAATCTTCAGTCGGTTCCGCGTTATC                                                       <  SRR4302547.1029734/94‑1 (MQ=60)
                                     CGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATC                                                       <  SRR4302547.6139528/94‑1 (MQ=60)
                                     CGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATC                                                       <  SRR4302547.989644/94‑1 (MQ=60)
                                      GACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCA                                                      >  SRR4302547.4171016/1‑94 (MQ=60)
                                       ACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAG                                                     <  SRR4302547.782517/94‑1 (MQ=60)
                                        CTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGT                                                    <  SRR4302547.5204045/94‑1 (MQ=60)
                                        CTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGT                                                    >  SRR4302547.3039289/1‑94 (MQ=60)
                                          TCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCC                                                  >  SRR4302547.510463/1‑94 (MQ=60)
                                          TCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCC                                                  <  SRR4302547.2089831/94‑1 (MQ=60)
                                           CACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCA                                                 <  SRR4302547.4935012/94‑1 (MQ=60)
                                            CCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCAT                                                <  SRR4302547.4249254/94‑1 (MQ=60)
                                            ACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCTAT                                                <  SRR4302547.6495276/94‑1 (MQ=60)
                                             CGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATT                                               <  SRR4302547.3649013/94‑1 (MQ=60)
                                             CGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATT                                               <  SRR4302547.5202453/94‑1 (MQ=60)
                                              GATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTT                                              >  SRR4302547.3529342/1‑94 (MQ=60)
                                              GATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTT                                              <  SRR4302547.6409275/94‑1 (MQ=60)
                                              GATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTT                                              >  SRR4302547.3291845/1‑94 (MQ=60)
                                               ATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTG                                             <  SRR4302547.5423906/94‑1 (MQ=60)
                                                 CGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTT                                           >  SRR4302547.439693/1‑94 (MQ=60)
                                                 CGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTT                                           <  SRR4302547.2485723/94‑1 (MQ=60)
                                                 CGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTT                                           <  SRR4302547.924435/94‑1 (MQ=60)
                                                  GCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTT                                          <  SRR4302547.2658643/94‑1 (MQ=60)
                                                   TTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTT                                         >  SRR4302547.2938921/1‑94 (MQ=60)
                                                    TTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTC                                        <  SRR4302547.4769172/94‑1 (MQ=60)
                                                    TTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTATC                                        <  SRR4302547.5387365/94‑1 (MQ=60)
                                                     TTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCT                                       <  SRR4302547.1398613/94‑1 (MQ=60)
                                                     TTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCT                                       <  SRR4302547.623740/94‑1 (MQ=60)
                                                      TCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTT                                      <  SRR4302547.2258514/94‑1 (MQ=60)
                                                      TCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTT                                      <  SRR4302547.3725564/94‑1 (MQ=60)
                                                        GTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCC                                    >  SRR4302547.6467739/1‑94 (MQ=60)
                                                        GTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCC                                    <  SRR4302547.1921936/94‑1 (MQ=60)
                                                         TCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCG                                   >  SRR4302547.4396490/1‑94 (MQ=60)
                                                          CTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGC                                  >  SRR4302547.6164683/1‑94 (MQ=60)
                                                           TTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCC                                 <  SRR4302547.858043/94‑1 (MQ=60)
                                                           TTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCC                                 >  SRR4302547.6740904/1‑94 (MQ=60)
                                                           TTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCC                                 >  SRR4302547.1738157/1‑94 (MQ=60)
                                                            TCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCA                                <  SRR4302547.1084011/94‑1 (MQ=60)
                                                              TGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATA                              >  SRR4302547.2993824/1‑94 (MQ=60)
                                                              TGCCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATA                              >  SRR4302547.827407/1‑94 (MQ=60)
                                                                CCTGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACG                            >  SRR4302547.2416704/1‑94 (MQ=60)
                                                                CCTGGCGAGCATGTTGAGAAGTAGCGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACG                            >  SRR4302547.5477220/1‑94 (MQ=60)
                                                                  TGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGAC                          <  SRR4302547.5118341/94‑1 (MQ=60)
                                                                  TGGCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGAC                          <  SRR4302547.2905178/94‑1 (MQ=60)
                                                                    GCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGT                        >  SRR4302547.5188567/1‑94 (MQ=60)
                                                                    GCGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGT                        >  SRR4302547.4576933/1‑94 (MQ=60)
                                                                     CGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTG                       <  SRR4302547.6181157/94‑1 (MQ=60)
                                                                     CGAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTG                       <  SRR4302547.1691727/94‑1 (MQ=60)
                                                                      GAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGC                      >  SRR4302547.3246846/1‑94 (MQ=60)
                                                                      GAGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGC                      >  SRR4302547.1859235/1‑94 (MQ=60)
                                                                       AGCATGTTGAGAAGTAGTGACGCGATAATCGCTGTAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCT                     <  SRR4302547.1007905/94‑1 (MQ=60)
                                                                       AGCATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCT                     >  SRR4302547.1162278/1‑94 (MQ=60)
                                                                         CATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTC                   <  SRR4302547.533888/94‑1 (MQ=60)
                                                                         CATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTC                   <  SRR4302547.895636/94‑1 (MQ=60)
                                                                          ATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCT                  >  SRR4302547.1654436/1‑94 (MQ=60)
                                                                          ATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCT                  >  SRR4302547.5291451/1‑94 (MQ=60)
                                                                          ATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCT                  >  SRR4302547.2824996/1‑94 (MQ=60)
                                                                          ATGTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCT                  <  SRR4302547.5484539/94‑1 (MQ=60)
                                                                           TGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTT                 >  SRR4302547.1347980/1‑94 (MQ=60)
                                                                            GTTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTC                <  SRR4302547.6618646/94‑1 (MQ=60)
                                                                             TTGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCA               >  SRR4302547.39004/1‑94 (MQ=60)
                                                                              TGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAG              >  SRR4302547.696621/1‑94 (MQ=60)
                                                                              TGAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAG              <  SRR4302547.3127126/94‑1 (MQ=60)
                                                                               GAGAAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGC             <  SRR4302547.6300765/94‑1 (MQ=60)
                                                                                 GAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAA           <  SRR4302547.266496/94‑1 (MQ=60)
                                                                                  AAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAAC          <  SRR4302547.4319879/94‑1 (MQ=60)
                                                                                  AAGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAAC          <  SRR4302547.5053996/94‑1 (MQ=60)
                                                                                   AGTAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACG         >  SRR4302547.2621702/1‑94 (MQ=60)
                                                                                    GTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGC        <  SRR4302547.1954651/94‑1 (MQ=60)
                                                                                     TAGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCG       >  SRR4302547.4023470/1‑94 (MQ=60)
                                                                                     TACTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCG       <  SRR4302547.378370/94‑1 (MQ=60)
                                                                                      AGTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTTCGCCATACGACGTGCTTCTTCAGCAACGCGA      >  SRR4302547.5281046/1‑94 (MQ=60)
                                                                                       GTGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGAC     <  SRR4302547.591746/94‑1 (MQ=60)
                                                                                        TGACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACG    <  SRR4302547.5755744/94‑1 (MQ=60)
                                                                                          ACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  >  SRR4302547.3900044/1‑94 (MQ=60)
                                                                                          ACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  <  SRR4302547.6267288/94‑1 (MQ=60)
                                                                                          ACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  <  SRR4302547.5714810/94‑1 (MQ=60)
                                                                                          ACGCGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  <  SRR4302547.5713309/94‑1 (MQ=60)
                                                                                          ACGCGATAATCGCGGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  <  SRR4302547.5429836/94‑1 (MQ=60)
                                                                                             |                                                                                          
GCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGGTTCCGCGTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTG  >  NC_000913/3312877‑3313060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑