Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,542,050 A→G L144P (CTG→CCG)  bioH ← BioH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,0500AG86.4% 654.6 / 86.1 249L144P (CTG→CCG) bioHBioH
Reads supporting (aligned to +/- strand):  ref base A (19/15);  new base G (104/111);  total (123/126)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 259 total aligned reads displayed.

GTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACG  >  NC_000913/3541959‑3542141
                                                                                           |                                                                                           
 tCACCCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCggg                                                                                           <  1:3347496/93‑1 (MQ=255)
  cAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGa                                                                                          >  1:5825606/1‑93 (MQ=255)
   aaCCTCCGGCATCGGTAACGCAAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACCCCAGGaa                                                                                         <  1:6537800/93‑1 (MQ=255)
    aCCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAAc                                                                                        <  2:3562267/93‑1 (MQ=255)
    aCCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAAc                                                                                        <  2:3686525/93‑1 (MQ=255)
       tCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGc                                                                                     >  1:126766/1‑93 (MQ=255)
       tCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGc                                                                                     >  2:2486150/1‑93 (MQ=255)
       tCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGc                                                                                     >  2:5046753/1‑93 (MQ=255)
       tCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGc                                                                                     >  2:4930691/1‑93 (MQ=255)
        ccGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCt                                                                                    <  1:1357202/93‑1 (MQ=255)
        ccGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCt                                                                                    >  1:1640994/1‑93 (MQ=255)
         cGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTc                                                                                   <  2:5940666/93‑1 (MQ=255)
            cATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCAc                                                                                <  2:2130311/93‑1 (MQ=255)
            cATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCAc                                                                                <  2:5796484/93‑1 (MQ=255)
            cATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCATGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCAc                                                                                >  2:3549978/1‑93 (MQ=255)
             aTCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACt                                                                               >  1:4347590/1‑93 (MQ=255)
             aTCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACt                                                                               >  2:4111661/1‑93 (MQ=255)
             aTCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACt                                                                               <  2:6615189/93‑1 (MQ=255)
               cGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGt                                                                             <  1:4752092/93‑1 (MQ=255)
               cGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGt                                                                             <  2:846768/93‑1 (MQ=255)
               cGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGt                                                                             <  2:698475/93‑1 (MQ=255)
                   aaCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGc                                                                         <  1:6034851/93‑1 (MQ=255)
                    aCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCt                                                                        <  2:1393453/93‑1 (MQ=255)
                    aCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCt                                                                        <  1:2300297/93‑1 (MQ=255)
                    aCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCt                                                                        <  1:4527037/93‑1 (MQ=255)
                    aCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACACt                                                                        >  2:3365568/1‑93 (MQ=255)
                      gCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGa                                                                      <  1:6321129/93‑1 (MQ=255)
                      cccAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGa                                                                      <  1:4892593/92‑1 (MQ=255)
                        cAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGaaa                                                                    >  1:6167827/1‑93 (MQ=255)
                         aGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGACGTTTCAGTCCACATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGaaaa                                                                   >  1:5622127/1‑93 (MQ=255)
                          gAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAat                                                                  >  2:3915872/1‑93 (MQ=255)
                           aaCGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAatc                                                                 <  1:1477279/93‑1 (MQ=255)
                             cGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAatcat                                                               <  2:6193946/93‑1 (MQ=255)
                             cGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAatcat                                                               <  2:5927126/93‑1 (MQ=255)
                              ggTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAatcatc                                                              <  2:3991560/93‑1 (MQ=255)
                              ggTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAatcatc                                                              <  1:54312/93‑1 (MQ=255)
                               gTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAAtcatca                                                             <  1:4648498/93‑1 (MQ=255)
                                ttttCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCAc                                                            >  2:190824/1‑93 (MQ=255)
                                ttttCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCAc                                                            <  2:2364687/93‑1 (MQ=255)
                                ttttCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCAc                                                            <  2:2094881/93‑1 (MQ=255)
                                ttttCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCAc                                                            <  1:499183/93‑1 (MQ=255)
                                   tcttcAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGa                                                         <  2:4789809/93‑1 (MQ=255)
                                     ttcAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGt                                                       <  1:3533286/93‑1 (MQ=255)
                                      tcAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGtt                                                      >  2:3041112/1‑93 (MQ=255)
                                      tcAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGtt                                                      <  2:4365460/93‑1 (MQ=255)
                                       cAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTtg                                                     >  2:5864110/1‑93 (MQ=255)
                                        aaCGCCCGCGCATCCTGGCGCGCTGTTTCAGTCCCCATGGTTTGTAGCGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTtgc                                                    >  1:5340303/1‑93 (MQ=255)
                                         aCGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTtgct                                                   <  2:5502805/93‑1 (MQ=255)
                                         aCGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTtgct                                                   >  1:4514802/1‑93 (MQ=255)
                                          cGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTtgctg                                                  >  2:1684756/1‑93 (MQ=255)
                                           gCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTtgctgc                                                 <  2:1086692/93‑1 (MQ=255)
                                            cccGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTgctgct                                                <  2:2028231/93‑1 (MQ=255)
                                             ccGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGctgctg                                               >  2:293288/1‑93 (MQ=255)
                                                 gcATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAAt                                           >  2:4889420/1‑93 (MQ=255)
                                                 gcATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAAt                                           >  1:3785032/1‑93 (MQ=255)
                                                 gcATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAAt                                           >  1:5514117/1‑93 (MQ=255)
                                                  cATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGACAATCATCACTGAGTTGCTGCTGAAATc                                          >  2:1225228/1‑93 (MQ=255)
                                                  cATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGGACGCTGAAAATCATCACTGAGTTGCTGCTGAAATc                                          >  1:935810/1‑93 (MQ=255)
                                                   aTCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATcc                                         <  2:5003792/93‑1 (MQ=255)
                                                    tCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATccc                                        >  2:252065/1‑93 (MQ=255)
                                                    tCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGATGAAATccc                                        >  2:5683659/1‑93 (MQ=255)
                                                      cTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTCCGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGc                                      <  1:2354000/93‑1 (MQ=255)
                                                       tGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGcc                                     <  1:1062500/93‑1 (MQ=255)
                                                       tGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGcc                                     <  2:6567151/93‑1 (MQ=255)
                                                         gcgcgcCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAg                                   <  1:2023425/93‑1 (MQ=255)
                                                         gcgcgcCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAg                                   <  1:4114534/93‑1 (MQ=255)
                                                          cgcgcCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGc                                  <  2:2067309/93‑1 (MQ=255)
                                                           gcgcCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCGCTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCa                                 <  2:3396902/93‑1 (MQ=255)
                                                           gcgcCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCATTGAGTTGCTGCTGAAATCCCGccacca                                 >  1:203137/1‑93 (MQ=255)
                                                             gcCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACg                               >  2:6489865/1‑93 (MQ=255)
                                                             gcCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACg                               >  1:1877793/1‑93 (MQ=255)
                                                              ccGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGt                              >  1:783393/1‑93 (MQ=255)
                                                              ccGTTTCAGTCCCCATGGCTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGCGTTGCTGCTGACATCCCGCCAGCACGt                              >  1:4203882/1‑93 (MQ=255)
                                                                gTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTcc                            <  1:6510514/93‑1 (MQ=255)
                                                                 tttCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCg                           >  1:6206251/1‑93 (MQ=255)
                                                                 tttCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCg                           >  1:2216813/1‑93 (MQ=255)
                                                                  ttCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCgg                          <  1:4919129/93‑1 (MQ=255)
                                                                  ttCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAACCCCGCCAGCACGTCCgg                          <  1:889518/93‑1 (MQ=255)
                                                                   tCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGt                         <  1:1576128/93‑1 (MQ=255)
                                                                    cAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGtt                        <  1:3268682/93‑1 (MQ=255)
                                                                      gTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGtttt                      >  2:459815/1‑93 (MQ=255)
                                                                       tCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTa                     <  1:5698370/93‑1 (MQ=255)
                                                                        ccccATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTAt                    >  2:1838444/1‑93 (MQ=255)
                                                                        ccccATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTAt                    >  2:4323356/1‑93 (MQ=255)
                                                                        ccccATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTAt                    <  1:2587143/93‑1 (MQ=255)
                                                                        ccccATGGTTTGTAACGCCGGAAGCCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTAt                    <  1:6104551/93‑1 (MQ=255)
                                                                           cATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATctc                 >  1:3851914/1‑91 (MQ=255)
                                                                           cATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATccc                 >  2:5378820/1‑93 (MQ=255)
                                                                            aTGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATcccc                <  1:2724594/93‑1 (MQ=255)
                                                                            aTGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATcccc                <  1:1922155/93‑1 (MQ=255)
                                                                              ggTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCgg              >  1:2510386/1‑93 (MQ=255)
                                                                              ggTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCgg              >  1:1176419/1‑93 (MQ=255)
                                                                                tttGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGcc            >  2:3221569/1‑93 (MQ=255)
                                                                                    tAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTc        >  2:1048992/1‑93 (MQ=255)
                                                                                      aCGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGt      <  1:3503531/93‑1 (MQ=255)
                                                                                      aCGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGt      <  2:447388/93‑1 (MQ=255)
                                                                                      aCGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGt      <  2:4176280/93‑1 (MQ=255)
                                                                                       cGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTc     >  1:5195393/1‑93 (MQ=255)
                                                                                        gCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCa    <  2:5143003/93‑1 (MQ=255)
                                                                                          cAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACg  <  1:4822620/93‑1 (MQ=255)
                                                                                           |                                                                                           
GTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACG  >  NC_000913/3541959‑3542141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  >  NC_000913/3541957‑3542143
                                                                                             |                                                                                             
ACGTCAACCTCCGGCATCGTTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCG                                                                                               >  SRR4302547.5711080/1‑94 (MQ=60)
ACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCG                                                                                               <  SRR4302547.2340767/94‑1 (MQ=60)
  GTCACCCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGG                                                                                             <  SRR4302547.5700130/94‑1 (MQ=60)
   TCACCCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGA                                                                                            <  SRR4302547.3415346/94‑1 (MQ=60)
   TCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGA                                                                                            >  SRR4302547.5946168/1‑94 (MQ=60)
   TCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGA                                                                                            >  SRR4302547.1415431/1‑94 (MQ=60)
   TCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGA                                                                                            <  SRR4302547.2008486/94‑1 (MQ=60)
     AACCTCCGGCATCGGTAACGCAAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACCCCAGGAAC                                                                                          <  SRR4302547.6673852/94‑1 (MQ=60)
      ACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACC                                                                                         >  SRR4302547.1953483/1‑94 (MQ=60)
       CCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCG                                                                                        <  SRR4302547.2601320/94‑1 (MQ=60)
        CTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGC                                                                                       <  SRR4302547.4510723/94‑1 (MQ=60)
        CTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGC                                                                                       >  SRR4302547.129063/1‑94 (MQ=60)
        CTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGC                                                                                       >  SRR4302547.917158/1‑94 (MQ=60)
        CTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGC                                                                                       >  SRR4302547.4766298/1‑94 (MQ=60)
         TCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCT                                                                                      >  SRR4302547.1673425/1‑94 (MQ=60)
         TCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCT                                                                                      <  SRR4302547.2670723/94‑1 (MQ=60)
          CCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTC                                                                                     <  SRR4302547.1384433/94‑1 (MQ=60)
            GGCATCGGTAACGCCAGAACGGTTTTCTTCAGCGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCA                                                                                   <  SRR4302547.3596312/94‑1 (MQ=60)
            GGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCA                                                                                   >  SRR4302547.1898634/1‑94 (MQ=60)
            GGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCA                                                                                   >  SRR4302547.1204707/1‑94 (MQ=60)
            GGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGGTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCA                                                                                   >  SRR4302547.2343217/1‑94 (MQ=60)
             GCATCGGTAACGTCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCAC                                                                                  <  SRR4302547.4617673/94‑1 (MQ=60)
             GCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCAC                                                                                  >  SRR4302547.6234564/1‑94 (MQ=60)
              CATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACT                                                                                 >  SRR4302547.4436943/1‑94 (MQ=60)
              CATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACT                                                                                 <  SRR4302547.1636449/94‑1 (MQ=60)
              CATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACT                                                                                 >  SRR4302547.3470690/1‑94 (MQ=60)
              CATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACT                                                                                 >  SRR4302547.4893911/1‑94 (MQ=60)
               ATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTG                                                                                <  SRR4302547.6720665/94‑1 (MQ=60)
               ATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTG                                                                                >  SRR4302547.2373435/1‑94 (MQ=60)
               ATCGGTAACCCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGGTTCTAACGCCGGGAACCGCTCCCCTG                                                                                >  SRR4302547.2405893/1‑94 (MQ=60)
                TCGGTAACGCCAGAACGGTTGTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGT                                                                               <  SRR4302547.3379378/94‑1 (MQ=60)
                 CGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTA                                                                              <  SRR4302547.4850579/94‑1 (MQ=60)
                 CGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTA                                                                              <  SRR4302547.6414516/94‑1 (MQ=60)
                    TAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGC                                                                           >  SRR4302547.675781/1‑94 (MQ=60)
                     AACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCT                                                                          <  SRR4302547.6160084/94‑1 (MQ=60)
                      ACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTG                                                                         <  SRR4302547.2346916/94‑1 (MQ=60)
                      ACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTG                                                                         <  SRR4302547.4620589/94‑1 (MQ=60)
                        GCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAA                                                                       <  SRR4302547.6452561/94‑1 (MQ=60)
                        CCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAA                                                                       <  SRR4302547.4993919/94‑1 (MQ=60)
                         CCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAA                                                                      >  SRR4302547.6295856/1‑94 (MQ=60)
                         CCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAA                                                                      >  SRR4302547.1318859/1‑94 (MQ=60)
                          CAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAA                                                                     >  SRR4302547.5971339/1‑94 (MQ=60)
                          CAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGACGTTTCAGTCCACATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAA                                                                     >  SRR4302547.5738153/1‑94 (MQ=60)
                           AGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAAT                                                                    <  SRR4302547.5692386/94‑1 (MQ=60)
                            GAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATC                                                                   >  SRR4302547.6114851/1‑94 (MQ=60)
                             AACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCA                                                                  >  SRR4302547.4940446/1‑94 (MQ=60)
                             AACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCA                                                                  <  SRR4302547.1506782/94‑1 (MQ=60)
                              ACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCAT                                                                 <  SRR4302547.2801153/94‑1 (MQ=60)
                              ACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCAT                                                                 <  SRR4302547.5773625/94‑1 (MQ=60)
                                GGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCA                                                               <  SRR4302547.55333/94‑1 (MQ=60)
                                GGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCA                                                               >  SRR4302547.1601226/1‑94 (MQ=60)
                                 GTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCAC                                                              <  SRR4302547.4744795/94‑1 (MQ=60)
                                  TTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACT                                                             <  SRR4302547.508285/94‑1 (MQ=60)
                                    TTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGA                                                           <  SRR4302547.2743742/94‑1 (MQ=60)
                                    TTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGA                                                           <  SRR4302547.882803/94‑1 (MQ=60)
                                       TTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTT                                                        <  SRR4302547.3605435/94‑1 (MQ=60)
                                         CAACGCCCGCGCATCCTGGCGCGCTGTTTCAGTCCCCATGGTTTGTAGCGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGC                                                      >  SRR4302547.5450383/1‑94 (MQ=60)
                                         CAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGC                                                      >  SRR4302547.5032300/1‑94 (MQ=60)
                                          AACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCT                                                     >  SRR4302547.4608083/1‑94 (MQ=60)
                                           ACGGCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTG                                                    <  SRR4302547.6476609/94‑1 (MQ=60)
                                           ACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTG                                                    >  SRR4302547.5912684/1‑94 (MQ=60)
                                             GCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCT                                                  >  SRR4302547.6328686/1‑94 (MQ=60)
                                               CCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGA                                                <  SRR4302547.475715/94‑1 (MQ=60)
                                                  CGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAAT                                             >  SRR4302547.5627952/1‑94 (MQ=60)
                                                  CGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAAT                                             >  SRR4302547.3862202/1‑94 (MQ=60)
                                                   GCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGGACGCTGAAAATCATCACTGAGTTGCTGCTGAAATC                                            >  SRR4302547.953922/1‑94 (MQ=60)
                                                     ATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCC                                          <  SRR4302547.44558/94‑1 (MQ=60)
                                                      TCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCG                                         <  SRR4302547.423029/94‑1 (MQ=60)
                                                       CCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGC                                        <  SRR4302547.6521378/94‑1 (MQ=60)
                                                        CTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTCCGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCC                                       <  SRR4302547.2401705/94‑1 (MQ=60)
                                                         TGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCA                                      <  SRR4302547.1717608/94‑1 (MQ=60)
                                                         TGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCA                                      >  SRR4302547.6163826/1‑94 (MQ=60)
                                                         TGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCA                                      <  SRR4302547.1083642/94‑1 (MQ=60)
                                                           GCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGC                                    <  SRR4302547.4198153/94‑1 (MQ=60)
                                                           GCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGC                                    <  SRR4302547.2063719/94‑1 (MQ=60)
                                                           GCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGATGCTGCTGAAATCCCGCCAGC                                    >  SRR4302547.3637049/1‑94 (MQ=60)
                                                            CGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCATTGAGTTGCTGCTGAAATCCCGCCACCA                                   >  SRR4302547.206936/1‑94 (MQ=60)
                                                             GCGCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCAC                                  <  SRR4302547.4971613/94‑1 (MQ=60)
                                                              CGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACG                                 >  SRR4302547.1914893/1‑94 (MQ=60)
                                                               GCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGT                                >  SRR4302547.4722064/1‑94 (MQ=60)
                                                               GCCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTAAGTTGCTGCTGAAATCCCGCCAGCACGT                                >  SRR4302547.1255193/1‑94 (MQ=60)
                                                               GCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGT                                >  SRR4302547.798201/1‑94 (MQ=60)
                                                               GCCGTTTCAGTCCCCATGGCTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGCGTTGCTGCTGACATCCCGCCAGCACGT                                >  SRR4302547.4289648/1‑94 (MQ=60)
                                                                CCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTC                               <  SRR4302547.3875222/94‑1 (MQ=60)
                                                                CCGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTC                               >  SRR4302547.5540304/1‑94 (MQ=60)
                                                                 CGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATTCCGCCAGCACGTCC                              >  SRR4302547.3843952/1‑94 (MQ=60)
                                                                 CGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCC                              <  SRR4302547.2067257/94‑1 (MQ=60)
                                                                 CGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCC                              >  SRR4302547.5214126/1‑94 (MQ=60)
                                                                 CGTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCC                              >  SRR4302547.1409717/1‑94 (MQ=60)
                                                                  GTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCG                             <  SRR4302547.1629308/94‑1 (MQ=60)
                                                                  GTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCG                             <  SRR4302547.6646059/94‑1 (MQ=60)
                                                                  GTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCG                             <  SRR4302547.5076968/94‑1 (MQ=60)
                                                                  GTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCG                             >  SRR4302547.6335103/1‑94 (MQ=60)
                                                                  GTTTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCG                             >  SRR4302547.2261456/1‑94 (MQ=60)
                                                                    TTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGT                           <  SRR4302547.5021011/94‑1 (MQ=60)
                                                                    TTCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAACCCCGCCAGCACGTCCGGT                           <  SRR4302547.906622/94‑1 (MQ=60)
                                                                    TTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGT                           <  SRR4302547.3955129/94‑1 (MQ=60)
                                                                     TCAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTT                          <  SRR4302547.1607500/94‑1 (MQ=60)
                                                                      CAGTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTT                         <  SRR4302547.3334090/94‑1 (MQ=60)
                                                                        GTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTA                       >  SRR4302547.4071875/1‑94 (MQ=60)
                                                                        GTCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTA                       <  SRR4302547.5479165/94‑1 (MQ=60)
                                                                         TCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTAT                      <  SRR4302547.246173/94‑1 (MQ=60)
                                                                         TCCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTAT                      <  SRR4302547.5816068/94‑1 (MQ=60)
                                                                          CCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATC                     <  SRR4302547.6286875/94‑1 (MQ=60)
                                                                          CCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATC                     >  SRR4302547.2340431/1‑94 (MQ=60)
                                                                          CCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATC                     <  SRR4302547.2082842/94‑1 (MQ=60)
                                                                          CCCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATC                     <  SRR4302547.2639348/94‑1 (MQ=60)
                                                                          CCCCATGGTTTGTAACGCCGGAAGCCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATC                     <  SRR4302547.6231184/94‑1 (MQ=60)
                                                                           CCCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCC                    >  SRR4302547.6669400/1‑94 (MQ=60)
                                                                            CCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCTC                   >  SRR4302547.3930325/1‑94 (MQ=60)
                                                                            CCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCC                   <  SRR4302547.4312135/94‑1 (MQ=60)
                                                                            CCATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCC                   >  SRR4302547.4645520/1‑94 (MQ=60)
                                                                             CATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCC                  <  SRR4302547.4998466/94‑1 (MQ=60)
                                                                             CATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCC                  >  SRR4302547.5630176/1‑94 (MQ=60)
                                                                             CATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGATTTATCCCC                  >  SRR4302547.1180709/1‑94 (MQ=60)
                                                                             CATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCC                  <  SRR4302547.5586699/94‑1 (MQ=60)
                                                                              ATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCG                 <  SRR4302547.2779297/94‑1 (MQ=60)
                                                                              ATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCG                 <  SRR4302547.1960218/94‑1 (MQ=60)
                                                                              ATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCG                 <  SRR4302547.2148173/94‑1 (MQ=60)
                                                                              ATGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCG                 >  SRR4302547.6337862/1‑94 (MQ=60)
                                                                               TGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGG                <  SRR4302547.100619/94‑1 (MQ=60)
                                                                               TGGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGG                >  SRR4302547.2561129/1‑94 (MQ=60)
                                                                               TGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGG                >  SRR4302547.1200189/1‑94 (MQ=60)
                                                                                GGTTTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGC               <  SRR4302547.922820/94‑1 (MQ=60)
                                                                                 GTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCC              <  SRR4302547.5788369/94‑1 (MQ=60)
                                                                                 GTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCC              >  SRR4302547.5333760/1‑94 (MQ=60)
                                                                                 GTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCC              >  SRR4302547.5141298/1‑94 (MQ=60)
                                                                                   TTGTAACGCCGGGATCCGCGCCACTGTACGCTGAAAATCATCACTGATTTGCTTCGGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCAC            <  SRR4302547.3449270/94‑1 (MQ=60)
                                                                                   TTGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCAC            >  SRR4302547.2058044/1‑94 (MQ=60)
                                                                                    TGTAACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACT           >  SRR4302547.3225288/1‑94 (MQ=60)
                                                                                        ACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTC       >  SRR4302547.5302318/1‑94 (MQ=60)
                                                                                        ACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTC       <  SRR4302547.3575032/94‑1 (MQ=60)
                                                                                        ACGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTC       <  SRR4302547.3433922/94‑1 (MQ=60)
                                                                                         CGCCGGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCA      <  SRR4302547.4261609/94‑1 (MQ=60)
                                                                                            CAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGA   <  SRR4302547.4922568/94‑1 (MQ=60)
                                                                                             GGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  >  SRR4302547.3368215/1‑94 (MQ=60)
                                                                                             GGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  >  SRR4302547.4461777/1‑94 (MQ=60)
                                                                                             GGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  <  SRR4302547.6614530/94‑1 (MQ=60)
                                                                                             GGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGGTTTATCCCCGGCCACTCGTCACGAG  >  SRR4302547.850986/1‑94 (MQ=60)
                                                                                             GGGAACCGCTCCACGGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  <  SRR4302547.153610/94‑1 (MQ=60)
                                                                                             AGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  >  SRR4302547.1332284/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAG  >  NC_000913/3541957‑3542143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑