Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,550,199 A→G intergenic (‑93/‑519) malP ← / → malT maltodextrin phosphorylase/MalT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,550,1990AG85.2% 632.6 / 98.5 250intergenic (‑93/‑519)malP/malTmaltodextrin phosphorylase/MalT
Reads supporting (aligned to +/- strand):  ref base A (14/23);  new base G (97/116);  total (111/139)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

Only 100 of 258 total aligned reads displayed.

GGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACA  >  NC_000913/3550108‑3550291
                                                                                           |                                                                                            
ggAGTTCCAGTTTTCTTAGATTTCCAACACAACGTTATCGCTAGTTTGCCAGGCTCTATGTTGTCCTTCCTCATCCTGCGGGGGATTAGGCAg                                                                                             <  1:3726553/93‑1 (MQ=255)
 gAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCCCATCCTGCGGGGGATTAGGCggg                                                                                            <  1:6127550/93‑1 (MQ=255)
  aGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCgggg                                                                                           >  2:2470553/1‑93 (MQ=255)
    ttCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGggag                                                                                         >  2:5503094/1‑93 (MQ=255)
    ttCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCGCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGggag                                                                                         <  2:4672485/93‑1 (MQ=255)
     tCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGggagg                                                                                        <  1:5874719/93‑1 (MQ=255)
     tCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGggagg                                                                                        <  1:1503235/93‑1 (MQ=255)
       cACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGgaggag                                                                                      >  2:1040287/1‑93 (MQ=255)
        aCTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGt                                                                                     <  2:4920652/93‑1 (MQ=255)
        aCTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGt                                                                                     <  2:4190974/93‑1 (MQ=255)
        aCTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGt                                                                                     <  2:4415565/93‑1 (MQ=255)
         cTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGtt                                                                                    >  1:182475/1‑93 (MQ=255)
          ttttCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTg                                                                                   >  2:2622315/1‑93 (MQ=255)
          ttttCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTg                                                                                   <  1:2813560/93‑1 (MQ=255)
           tttCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGc                                                                                  >  2:6351744/1‑93 (MQ=255)
           tttCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGc                                                                                  <  2:2083891/93‑1 (MQ=255)
            ttCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCg                                                                                 <  2:5696488/93‑1 (MQ=255)
             tCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCgg                                                                                <  2:362758/93‑1 (MQ=255)
             tCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCgg                                                                                <  2:3583689/93‑1 (MQ=255)
              cTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCggg                                                                               >  2:3846631/1‑93 (MQ=255)
               ttAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCgggg                                                                              >  1:1656543/1‑93 (MQ=255)
                tAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGa                                                                             <  1:3204072/93‑1 (MQ=255)
                tAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGa                                                                             <  1:893710/93‑1 (MQ=255)
                  gATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATg                                                                           >  1:1019305/1‑93 (MQ=255)
                    ttttCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAg                                                                         <  2:1809870/93‑1 (MQ=255)
                    ttttCAACACAACGTTATCGCTAGGTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAg                                                                         <  2:1855920/93‑1 (MQ=255)
                     tttCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGc                                                                        <  2:5032622/93‑1 (MQ=255)
                       tCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCaa                                                                      <  1:4281093/93‑1 (MQ=255)
                        cAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAg                                                                     <  1:5345873/93‑1 (MQ=255)
                         aaCACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAgg                                                                    <  2:5125011/93‑1 (MQ=255)
                         aaCACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAgg                                                                    >  1:2070714/1‑93 (MQ=255)
                          acacAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGa                                                                   >  1:4131018/1‑93 (MQ=255)
                            agaaCGTAATCGCTAGTTTGCCAGGCTCGATGTTAACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGaaa                                                                 <  2:1013542/91‑1 (MQ=255)
                            acaACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGaaa                                                                 >  2:2536122/1‑93 (MQ=255)
                            acaACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGaaa                                                                 <  1:1424410/93‑1 (MQ=255)
                            acaACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGaaa                                                                 >  1:4168090/1‑93 (MQ=255)
                             caACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAt                                                                <  2:4583772/93‑1 (MQ=255)
                             caACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAt                                                                >  2:3241465/1‑93 (MQ=255)
                             caACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAt                                                                >  2:5626701/1‑93 (MQ=255)
                              aaCGTTATCGCTAGTTTGCCAGGCTCGATGTTTACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAAtg                                                               <  1:854046/93‑1 (MQ=255)
                              aaCGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGGTGAGCAAGGAAAtg                                                               >  2:3503068/1‑93 (MQ=255)
                               aCGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAtgt                                                              >  1:1621063/1‑93 (MQ=255)
                                   tATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGAtc                                                          <  2:3767882/93‑1 (MQ=255)
                                       gCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCaa                                                      >  1:5762619/1‑93 (MQ=255)
                                        cTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAAc                                                     >  2:5816696/1‑93 (MQ=255)
                                        cTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAAc                                                     <  1:1174676/93‑1 (MQ=255)
                                         tAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAAcc                                                    <  2:114332/93‑1 (MQ=255)
                                          aGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCa                                                   <  1:3472379/93‑1 (MQ=255)
                                          aGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCa                                                   >  1:3948461/1‑93 (MQ=255)
                                           gTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCAc                                                  >  2:5749391/1‑93 (MQ=255)
                                            tttGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACt                                                 <  2:2315698/93‑1 (MQ=255)
                                             ttGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACtt                                                <  1:6090959/93‑1 (MQ=255)
                                               gCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTaa                                              >  1:3378248/1‑93 (MQ=255)
                                               gCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTaa                                              <  2:6183843/93‑1 (MQ=255)
                                                  aGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGc                                           <  1:1351261/93‑1 (MQ=255)
                                                  aGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGc                                           >  2:3367936/1‑93 (MQ=255)
                                                   ggCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCt                                          <  2:6010035/93‑1 (MQ=255)
                                                   ggCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCt                                          >  2:214575/1‑93 (MQ=255)
                                                     cTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAg                                        <  1:4646017/93‑1 (MQ=255)
                                                      tCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGt                                       >  1:1991969/1‑93 (MQ=255)
                                                      tCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGt                                       <  1:4705425/93‑1 (MQ=255)
                                                        gATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGc                                     >  1:1467611/1‑93 (MQ=255)
                                                               aCCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACcac                              <  2:2864189/93‑1 (MQ=255)
                                                               aCCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACcac                              <  1:3834295/93‑1 (MQ=255)
                                                               aCCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACcac                              >  1:4462916/1‑93 (MQ=255)
                                                                ccTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACcaca                             <  1:6584841/93‑1 (MQ=255)
                                                                 cTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAg                            <  2:3090009/93‑1 (MQ=255)
                                                                 cTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGACATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAg                            <  1:6641273/93‑1 (MQ=255)
                                                                  ttCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAgg                           >  1:5648944/1‑93 (MQ=255)
                                                                   tCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGa                          <  1:2483661/93‑1 (MQ=255)
                                                                   tCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGa                          <  1:361792/93‑1 (MQ=255)
                                                                   tCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGa                          <  1:3232366/93‑1 (MQ=255)
                                                                    ccTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAt                         >  1:2202341/1‑93 (MQ=255)
                                                                     cTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAtt                        <  1:5911462/93‑1 (MQ=255)
                                                                      tCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTa                       >  1:5515592/1‑93 (MQ=255)
                                                                       cATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAg                      <  2:6406160/93‑1 (MQ=255)
                                                                        aTCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGc                     <  1:5319888/93‑1 (MQ=255)
                                                                        aTCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGc                     <  1:2619126/93‑1 (MQ=255)
                                                                         tCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCa                    >  1:3031305/1‑93 (MQ=255)
                                                                         tCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCa                    >  1:5015953/1‑93 (MQ=255)
                                                                         tCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAAGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAAGATTAGCa                    >  1:5120117/1‑93 (MQ=255)
                                                                           cTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatc                  >  2:13582/1‑93 (MQ=255)
                                                                            tGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatca                 >  1:4435191/1‑93 (MQ=255)
                                                                             gCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatcaa                <  2:884941/93‑1 (MQ=255)
                                                                             gCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatcaa                <  2:3904844/93‑1 (MQ=255)
                                                                              cGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatcaaa               <  2:4027870/93‑1 (MQ=255)
                                                                               gggggATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatcaaat              <  2:711517/93‑1 (MQ=255)
                                                                                ggggATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatcaaatc             <  2:472462/93‑1 (MQ=255)
                                                                                  ggATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCatcaaatcaa           <  2:5309783/93‑1 (MQ=255)
                                                                                   gATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAAt          >  1:6149455/1‑93 (MQ=255)
                                                                                   gATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAAt          <  2:774421/93‑1 (MQ=255)
                                                                                    aTTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATg         <  2:4045945/93‑1 (MQ=255)
                                                                                    aTTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATg         <  1:5459122/93‑1 (MQ=255)
                                                                                    aTTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATg         <  2:6440911/93‑1 (MQ=255)
                                                                                     ttAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGc        <  1:1094366/93‑1 (MQ=255)
                                                                                      tAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCa       <  1:969560/93‑1 (MQ=255)
                                                                                       aGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCaa      <  1:2432015/93‑1 (MQ=255)
                                                                                        ggCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAAt     >  2:5013467/1‑93 (MQ=255)
                                                                                        ggCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAAt     <  1:677105/93‑1 (MQ=255)
                                                                                        ggCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAAt     >  1:212478/1‑93 (MQ=255)
                                                                                           |                                                                                            
GGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACA  >  NC_000913/3550108‑3550291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  >  NC_000913/3550106‑3550292
                                                                                             |                                                                                             
TAGGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCG                                                                                               <  SRR4302547.5967365/94‑1 (MQ=60)
TAGGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCG                                                                                               <  SRR4302547.6052845/94‑1 (MQ=60)
 AGGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGG                                                                                              >  SRR4302547.125050/1‑94 (MQ=60)
  GGAGTTCCAGTTTTCTTAGATTTCCAACACAACGTTATCGCTAGTTTGCCAGGCTCTATGTTGTCCTTCCTCATCCTGCGGGGGATTAGGCAGG                                                                                             <  SRR4302547.3802622/94‑1 (MQ=60)
  GGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGG                                                                                             <  SRR4302547.2184116/94‑1 (MQ=60)
   GAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGG                                                                                            <  SRR4302547.1169456/94‑1 (MQ=60)
   GAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCCCATCCTGCGGGGGATTAGGCGGGG                                                                                            <  SRR4302547.6254661/94‑1 (MQ=60)
    AGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGG                                                                                           >  SRR4302547.2372861/1‑94 (MQ=60)
       TCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGA                                                                                        <  SRR4302547.5996331/94‑1 (MQ=60)
       TCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGA                                                                                        <  SRR4302547.1533202/94‑1 (MQ=60)
        CCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAG                                                                                       >  SRR4302547.1792610/1‑94 (MQ=60)
        CCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTCGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAG                                                                                       >  SRR4302547.4227426/1‑94 (MQ=60)
          ACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTT                                                                                     >  SRR4302547.4788623/1‑94 (MQ=60)
          ACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTT                                                                                     >  SRR4302547.185901/1‑94 (MQ=60)
          ACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTT                                                                                     <  SRR4302547.3286699/94‑1 (MQ=60)
           CTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTG                                                                                    >  SRR4302547.1899197/1‑94 (MQ=60)
           CTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTG                                                                                    <  SRR4302547.1044570/94‑1 (MQ=60)
           CTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTG                                                                                    <  SRR4302547.6143724/94‑1 (MQ=60)
            TTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGC                                                                                   <  SRR4302547.2869848/94‑1 (MQ=60)
             TTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCG                                                                                  <  SRR4302547.1286413/94‑1 (MQ=60)
             TTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCG                                                                                  <  SRR4302547.3633764/94‑1 (MQ=60)
              TTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGG                                                                                 >  SRR4302547.4267954/1‑94 (MQ=60)
              TTCTTAGATTTTCAACACAACGTTATCGCTAGTCTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGG                                                                                 >  SRR4302547.1855757/1‑94 (MQ=60)
               TCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGG                                                                                >  SRR4302547.6682419/1‑94 (MQ=60)
                CTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGG                                                                               >  SRR4302547.2487435/1‑94 (MQ=60)
                CTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGG                                                                               >  SRR4302547.3799244/1‑94 (MQ=60)
                CTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGG                                                                               >  SRR4302547.1689216/1‑94 (MQ=60)
                 TTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGA                                                                              >  SRR4302547.1145666/1‑94 (MQ=60)
                 TTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGGTGCGGGGA                                                                              >  SRR4302547.5519148/1‑94 (MQ=60)
                  TAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGAT                                                                             <  SRR4302547.1464078/94‑1 (MQ=60)
                  TAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGAT                                                                             <  SRR4302547.2914838/94‑1 (MQ=60)
                  TAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGAT                                                                             <  SRR4302547.3267974/94‑1 (MQ=60)
                  TAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGAT                                                                             <  SRR4302547.910894/94‑1 (MQ=60)
                  TAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGAT                                                                             <  SRR4302547.1562723/94‑1 (MQ=60)
                  TAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTCCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGAT                                                                             <  SRR4302547.211760/94‑1 (MQ=60)
                   AGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATG                                                                            >  SRR4302547.1039275/1‑94 (MQ=60)
                         TCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAG                                                                      <  SRR4302547.6738852/94‑1 (MQ=60)
                         TCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAG                                                                      <  SRR4302547.4369031/94‑1 (MQ=60)
                          CAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGG                                                                     >  SRR4302547.2112050/1‑94 (MQ=60)
                          CAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGG                                                                     <  SRR4302547.5456087/94‑1 (MQ=60)
                          CAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGG                                                                     <  SRR4302547.6240174/94‑1 (MQ=60)
                           AACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGA                                                                    >  SRR4302547.4215066/1‑94 (MQ=60)
                            ACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGGGCAAGGAA                                                                   >  SRR4302547.662346/1‑94 (MQ=60)
                             CACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAA                                                                  >  SRR4302547.1503823/1‑94 (MQ=60)
                             CACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAA                                                                  >  SRR4302547.4252987/1‑94 (MQ=60)
                              ACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAT                                                                 <  SRR4302547.1452922/94‑1 (MQ=60)
                              ACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAT                                                                 >  SRR4302547.1387088/1‑94 (MQ=60)
                               CAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATG                                                                <  SRR4302547.2782197/94‑1 (MQ=60)
                               CAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATG                                                                <  SRR4302547.4560063/94‑1 (MQ=60)
                                AACGTTATCGCTAGTTTGCCAGGCTCGATGTTTACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGT                                                               <  SRR4302547.870365/94‑1 (MQ=60)
                                AACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGT                                                               >  SRR4302547.1653142/1‑94 (MQ=60)
                                 ACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTG                                                              >  SRR4302547.4872255/1‑94 (MQ=60)
                                   GTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGAT                                                            >  SRR4302547.2173668/1‑94 (MQ=60)
                                   GTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGAT                                                            <  SRR4302547.977877/94‑1 (MQ=60)
                                    TTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATC                                                           <  SRR4302547.330859/94‑1 (MQ=60)
                                     TATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCT                                                          >  SRR4302547.1570080/1‑94 (MQ=60)
                                      ATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTC                                                         >  SRR4302547.3976643/1‑94 (MQ=60)
                                        CGCTATTTTGCCAGGCTCGATGATGACCTTACTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGGAATGTGATATCAA                                                       >  SRR4302547.851266/1‑94 (MQ=60)
                                        CGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAA                                                       >  SRR4302547.4246377/1‑94 (MQ=60)
                                        CGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAA                                                       >  SRR4302547.5881795/1‑94 (MQ=60)
                                         GCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAAC                                                      >  SRR4302547.5162054/1‑94 (MQ=60)
                                         GCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAAC                                                      >  SRR4302547.2623558/1‑94 (MQ=60)
                                          CTAGTTTGCCAGGTTCGATGTTGGCCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACC                                                     <  SRR4302547.2404934/94‑1 (MQ=60)
                                          CTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACC                                                     <  SRR4302547.1198412/94‑1 (MQ=60)
                                          CTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACC                                                     >  SRR4302547.1498747/1‑94 (MQ=60)
                                           TAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCA                                                    >  SRR4302547.4028773/1‑94 (MQ=60)
                                            AGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCAC                                                   <  SRR4302547.3543193/94‑1 (MQ=60)
                                            AGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCAC                                                   <  SRR4302547.5964735/94‑1 (MQ=60)
                                             GTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACT                                                  <  SRR4302547.6233462/94‑1 (MQ=60)
                                              TTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTT                                                 >  SRR4302547.3581370/1‑94 (MQ=60)
                                               TTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTA                                                <  SRR4302547.6217308/94‑1 (MQ=60)
                                                TGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAA                                               >  SRR4302547.3446889/1‑94 (MQ=60)
                                                  CCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGGCATCTCAACCACTTAAAG                                             >  SRR4302547.3250230/1‑94 (MQ=60)
                                                  CCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAG                                             <  SRR4302547.3427692/94‑1 (MQ=60)
                                                   CAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGC                                            >  SRR4302547.5496868/1‑94 (MQ=60)
                                                    AGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCT                                           >  SRR4302547.3839574/1‑94 (MQ=60)
                                                    AGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCT                                           <  SRR4302547.1378397/94‑1 (MQ=60)
                                                    AGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCT                                           >  SRR4302547.5725859/1‑94 (MQ=60)
                                                     GGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTA                                          >  SRR4302547.6129093/1‑94 (MQ=60)
                                                      GCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAG                                         >  SRR4302547.5971578/1‑94 (MQ=60)
                                                       CTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGT                                        >  SRR4302547.2031534/1‑94 (MQ=60)
                                                       CTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGT                                        <  SRR4302547.4742236/94‑1 (MQ=60)
                                                        TCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTG                                       >  SRR4302547.6018225/1‑94 (MQ=60)
                                                        TCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTG                                       <  SRR4302547.5621611/94‑1 (MQ=60)
                                                        TCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTG                                       <  SRR4302547.4802867/94‑1 (MQ=60)
                                                         CGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGC                                      >  SRR4302547.1496935/1‑94 (MQ=60)
                                                         CGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGC                                      >  SRR4302547.5442794/1‑94 (MQ=60)
                                                         CGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGC                                      >  SRR4302547.3818955/1‑94 (MQ=60)
                                                          GATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCA                                     >  SRR4302547.1412836/1‑94 (MQ=60)
                                                          GATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCA                                     >  SRR4302547.5133410/1‑94 (MQ=60)
                                                             GTTGACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAAC                                  >  SRR4302547.4558101/1‑94 (MQ=60)
                                                               TGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCA                                <  SRR4302547.877294/94‑1 (MQ=60)
                                                               TCACCTTCCTCATCCTGCGAGTGATTAGGCGGGGAGGAGTTGCGGTGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCA                                <  SRR4302547.3455352/94‑1 (MQ=60)
                                                                GACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCAC                               >  SRR4302547.4555145/1‑94 (MQ=60)
                                                                 ACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACA                              <  SRR4302547.817840/94‑1 (MQ=60)
                                                                 ACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACA                              <  SRR4302547.2879595/94‑1 (MQ=60)
                                                                 ACCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACA                              <  SRR4302547.3912397/94‑1 (MQ=60)
                                                                  CCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAG                             <  SRR4302547.6721951/94‑1 (MQ=60)
                                                                  CCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAG                             <  SRR4302547.4536605/94‑1 (MQ=60)
                                                                  CCTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAG                             <  SRR4302547.680667/94‑1 (MQ=60)
                                                                   CTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGG                            <  SRR4302547.2220424/94‑1 (MQ=60)
                                                                   CTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGG                            >  SRR4302547.5765543/1‑94 (MQ=60)
                                                                   CTTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGG                            <  SRR4302547.4034377/94‑1 (MQ=60)
                                                                   CTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGACATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGG                            <  SRR4302547.6779516/94‑1 (MQ=60)
                                                                    TTCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAAACACAGGA                           >  SRR4302547.2032960/1‑94 (MQ=60)
                                                                     TCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAT                          <  SRR4302547.2533855/94‑1 (MQ=60)
                                                                     TCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAT                          <  SRR4302547.368513/94‑1 (MQ=60)
                                                                     TCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAT                          <  SRR4302547.3296880/94‑1 (MQ=60)
                                                                     TCCTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAT                          <  SRR4302547.5730291/94‑1 (MQ=60)
                                                                     TCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGAT                          >  SRR4302547.2246584/1‑94 (MQ=60)
                                                                       CTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTA                        <  SRR4302547.6033898/94‑1 (MQ=60)
                                                                       CTCATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTA                        >  SRR4302547.5629456/1‑94 (MQ=60)
                                                                          ATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCA                     >  SRR4302547.3091585/1‑94 (MQ=60)
                                                                          ATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCA                     <  SRR4302547.679910/94‑1 (MQ=60)
                                                                          ATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCA                     <  SRR4302547.5429574/94‑1 (MQ=60)
                                                                          ATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCA                     <  SRR4302547.2671900/94‑1 (MQ=60)
                                                                          ATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCA                     >  SRR4302547.5119630/1‑94 (MQ=60)
                                                                          ATCCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAAAGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAAGATTAGCA                     >  SRR4302547.5225602/1‑94 (MQ=60)
                                                                            CCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATC                   <  SRR4302547.6257274/94‑1 (MQ=60)
                                                                            CCTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATC                   <  SRR4302547.2073520/94‑1 (MQ=60)
                                                                             CTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCA                  <  SRR4302547.3190090/94‑1 (MQ=60)
                                                                             CTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCA                  <  SRR4302547.4472609/94‑1 (MQ=60)
                                                                             CTGCGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCA                  >  SRR4302547.4526880/1‑94 (MQ=60)
                                                                                CGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAAT               <  SRR4302547.1667141/94‑1 (MQ=60)
                                                                                CGGGGGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAAT               >  SRR4302547.5329020/1‑94 (MQ=60)
                                                                                    GGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAAT           >  SRR4302547.6277040/1‑94 (MQ=60)
                                                                                    GGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAAT           <  SRR4302547.4743896/94‑1 (MQ=60)
                                                                                    GGATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATGAGCATCAAATCAAT           <  SRR4302547.993470/94‑1 (MQ=60)
                                                                                     GATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATG          <  SRR4302547.5872683/94‑1 (MQ=60)
                                                                                      ATTAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGC         <  SRR4302547.5571912/94‑1 (MQ=60)
                                                                                       TTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCA        <  SRR4302547.1116309/94‑1 (MQ=60)
                                                                                        TAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAA       >  SRR4302547.4473160/1‑94 (MQ=60)
                                                                                        TAGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAA       <  SRR4302547.988383/94‑1 (MQ=60)
                                                                                         AGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAAT      >  SRR4302547.216487/1‑94 (MQ=60)
                                                                                         AGGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAAT      <  SRR4302547.2481217/94‑1 (MQ=60)
                                                                                         AGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAAT      >  SRR4302547.5440714/1‑94 (MQ=60)
                                                                                          GGCGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATA     <  SRR4302547.689576/94‑1 (MQ=60)
                                                                                          GGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATA     >  SRR4302547.96633/1‑94 (MQ=60)
                                                                                            CGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACA   <  SRR4302547.6434493/94‑1 (MQ=60)
                                                                                            CGGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACA   <  SRR4302547.5976198/94‑1 (MQ=60)
                                                                                             GGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  <  SRR4302547.1739752/94‑1 (MQ=60)
                                                                                             GGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  >  SRR4302547.1856787/1‑94 (MQ=60)
                                                                                             GGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  >  SRR4302547.3856382/1‑94 (MQ=60)
                                                                                             GGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  <  SRR4302547.1219236/94‑1 (MQ=60)
                                                                                             AGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  <  SRR4302547.2652981/94‑1 (MQ=60)
                                                                                             AGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  <  SRR4302547.3924946/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAGGAGTTCCACTTTTCTTAGATTTTCAACACAACGTTATCGCTAGTTTGCCAGGCTCGATGTTGACCTTCCTCATCCTGCGGGGGATTAGGCAGGGAGGAGTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAG  >  NC_000913/3550106‑3550292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑