Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,558,932 2 bp→GC coding (153‑154/831 nt) glpG ← GlpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,558,9320CG99.6% 921.3 / NA 258V52L (GTG→CTG) glpGGlpG
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (127/130);  total (127/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,558,9330GC99.6% 924.9 / NA 258D51E (GAC→GAG) glpGGlpG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (127/130);  minor base A (0/1);  total (127/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTG  >  NC_000913/3558845‑3559019
                                                                                       ||                                                                                      
 aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGc                                                                                   >  2:3138636/1‑93 (MQ=255)
 aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGGTTTCGAGAAAACGCGCCAGCTCCGc                                                                                   >  1:6566840/1‑93 (MQ=255)
    aTGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCg                                                                                >  2:1306426/1‑93 (MQ=255)
     tGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGt                                                                               <  2:3589578/93‑1 (MQ=255)
      gCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTa                                                                              >  1:2540632/1‑93 (MQ=255)
      gCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTAGAGAAAACGCGCCAGCTCCGCCCGTa                                                                              >  2:5143695/1‑93 (MQ=255)
       cAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAc                                                                             <  2:1165873/93‑1 (MQ=255)
       cAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAc                                                                             >  2:1399390/1‑93 (MQ=255)
       cAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAc                                                                             >  2:6334420/1‑93 (MQ=255)
       cAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAc                                                                             <  1:3181176/93‑1 (MQ=255)
        aGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg                                                                            >  2:467892/1‑93 (MQ=255)
        aGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg                                                                            >  1:4341926/1‑93 (MQ=255)
         ggCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcgc                                                                           <  1:4422546/93‑1 (MQ=255)
         ggCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcgc                                                                           <  2:1711460/93‑1 (MQ=255)
            cACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt                                                                        <  1:3720587/93‑1 (MQ=255)
            cACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt                                                                        >  1:1241382/1‑93 (MQ=255)
            cACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt                                                                        <  2:837563/93‑1 (MQ=255)
            cACGGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt                                                                        <  1:1432980/93‑1 (MQ=255)
             aCTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTc                                                                       >  1:122971/1‑93 (MQ=255)
              cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg                                                                      >  1:2820135/1‑93 (MQ=255)
              cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg                                                                      >  2:144240/1‑93 (MQ=255)
               tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCgg                                                                     >  1:6266405/1‑93 (MQ=255)
                  cGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGATTTCGAGAAAACGCGCCAGCTCCGCCCGTACCCGCTCGGCCt                                                                  >  1:2598058/1‑93 (MQ=255)
                     tATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg                                                               >  2:2166699/1‑93 (MQ=255)
                     tATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg                                                               >  1:4545173/1‑93 (MQ=255)
                      aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTgggc                                                              >  1:5465927/1‑92 (MQ=255)
                      aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGa                                                              >  1:6383356/1‑93 (MQ=255)
                      aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGa                                                              >  1:3500083/1‑93 (MQ=255)
                        ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt                                                            >  2:3027020/1‑93 (MQ=255)
                        ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt                                                            >  2:4509430/1‑93 (MQ=255)
                        ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt                                                            <  1:6196573/93‑1 (MQ=255)
                        ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt                                                            >  1:2100779/1‑93 (MQ=255)
                         gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTc                                                           <  2:2577660/93‑1 (MQ=255)
                         gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTc                                                           <  1:5880238/93‑1 (MQ=255)
                          cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCa                                                          <  2:6063839/93‑1 (MQ=255)
                            tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                        >  2:338444/1‑93 (MQ=255)
                            ggcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                        <  1:1792625/92‑1 (MQ=255)
                             gcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATcc                                                       <  1:1709271/93‑1 (MQ=255)
                             gcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATcc                                                       <  2:182064/93‑1 (MQ=255)
                                tgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgcc                                                    >  1:2490005/1‑93 (MQ=255)
                                tgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgcc                                                    >  1:300502/1‑93 (MQ=255)
                                 gccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgcca                                                   <  2:725534/93‑1 (MQ=255)
                                   cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagc                                                 >  1:2852802/1‑93 (MQ=255)
                                    aGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcc                                                >  1:821841/1‑93 (MQ=255)
                                      cTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGccagccag                                              <  2:5452641/93‑1 (MQ=255)
                                       tCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                             <  2:113296/93‑1 (MQ=255)
                                       tCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                             <  1:1099419/93‑1 (MQ=255)
                                          ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                          >  1:973675/1‑93 (MQ=255)
                                          ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                          <  1:565181/93‑1 (MQ=255)
                                           cgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATc                                         <  1:4810776/93‑1 (MQ=255)
                                             ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTCCGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                       <  1:1578056/93‑1 (MQ=255)
                                             ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                       <  2:2400434/93‑1 (MQ=255)
                                             ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                       >  2:572006/1‑93 (MQ=255)
                                                gATAGCGCGGATCTGCCGGGTTTTCGAGAAACCGCCCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt                                    <  1:4916274/93‑1 (MQ=255)
                                                gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt                                    >  2:6212235/1‑93 (MQ=255)
                                                gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGAAATCGCttt                                    >  1:2459986/1‑93 (MQ=255)
                                                 aTAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg                                   >  2:2312688/1‑93 (MQ=255)
                                                  tAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTgg                                  <  2:3534357/93‑1 (MQ=255)
                                                   aaCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt                                 >  2:1495993/1‑93 (MQ=255)
                                                   aaCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt                                 <  2:4909987/93‑1 (MQ=255)
                                                    aCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCCGACATCGCTTTGGtt                                >  1:4741260/1‑93 (MQ=255)
                                                    aCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt                                <  2:6128902/93‑1 (MQ=255)
                                                       cgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtg                             <  1:1988731/93‑1 (MQ=255)
                                                        ggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgt                            >  2:5048299/1‑93 (MQ=255)
                                                        ggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgt                            <  2:109865/93‑1 (MQ=255)
                                                        ggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgt                            <  1:4148151/93‑1 (MQ=255)
                                                         gATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgtt                           >  1:5230610/1‑93 (MQ=255)
                                                         gATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgtt                           <  2:69143/93‑1 (MQ=255)
                                                          aTCTGCCGGGTTGTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgttg                          <  2:6501188/93‑1 (MQ=255)
                                                             tGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                       <  1:3283469/93‑1 (MQ=255)
                                                             tGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                       <  1:5374622/93‑1 (MQ=255)
                                                                cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAt                    >  2:925523/1‑93 (MQ=255)
                                                                cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAt                    >  1:712370/1‑93 (MQ=255)
                                                                cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAt                    >  1:3592248/1‑93 (MQ=255)
                                                                  ggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCg                  >  2:6171960/1‑93 (MQ=255)
                                                                  ggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCg                  >  2:5991069/1‑93 (MQ=255)
                                                                   gTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGt                 >  1:5974739/1‑93 (MQ=255)
                                                                   gTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGt                 <  1:5086268/93‑1 (MQ=255)
                                                                   gTTTTCGAGAAAACGCGCCAGCTCCGCCCGGACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGt                 <  2:5297675/93‑1 (MQ=255)
                                                                    ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTg                <  2:5965717/93‑1 (MQ=255)
                                                                     tttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa               >  2:3665878/1‑93 (MQ=255)
                                                                       tCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAgg             <  2:4834563/93‑1 (MQ=255)
                                                                        cGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa            <  2:2497530/93‑1 (MQ=255)
                                                                        cGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa            <  1:2307180/93‑1 (MQ=255)
                                                                        cGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa            <  2:3319910/93‑1 (MQ=255)
                                                                         gagaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAt           <  1:2729250/93‑1 (MQ=255)
                                                                          agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATa          >  1:6333517/1‑93 (MQ=255)
                                                                            aaaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAac        <  1:100747/93‑1 (MQ=255)
                                                                             aaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAaca       <  2:5890368/93‑1 (MQ=255)
                                                                              aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAacac      <  2:2985015/93‑1 (MQ=255)
                                                                              aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCCTTGGTTATGTTGTTGAATCGTGAGGATAacac      <  1:3897218/93‑1 (MQ=255)
                                                                               aCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAcc     >  1:5746021/1‑93 (MQ=255)
                                                                               aCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAcc     >  2:4372942/1‑93 (MQ=255)
                                                                                cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc    <  2:5220610/93‑1 (MQ=255)
                                                                                cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc    <  1:4687661/93‑1 (MQ=255)
                                                                                cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc    <  2:3991616/93‑1 (MQ=255)
                                                                                cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc    <  1:9292/93‑1 (MQ=255)
                                                                                  cgcCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTg  <  2:3725551/93‑1 (MQ=255)
                                                                                  cgcCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTg  <  2:6450912/93‑1 (MQ=255)
                                                                                  cgcCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTg  <  2:321011/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTG  >  NC_000913/3558845‑3559019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCG  >  NC_000913/3558839‑3559021
                                                                                             |                                                                                         
AACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAG                                                                                           >  SRR4302547.2251965/1‑94 (MQ=60)
AACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAG                                                                                           >  SRR4302547.2777271/1‑94 (MQ=60)
AACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAG                                                                                           >  SRR4302547.3009323/1‑94 (MQ=60)
     CGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCG                                                                                      >  SRR4302547.4466258/1‑94 (MQ=60)
     CGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCG                                                                                      >  SRR4302547.4956454/1‑94 (MQ=60)
      GATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGC                                                                                     <  SRR4302547.1992886/94‑1 (MQ=60)
      GATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGC                                                                                     <  SRR4302547.3036245/94‑1 (MQ=60)
      GATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGGTTTCGAGAAAACGCGCCAGCTCCGC                                                                                     >  SRR4302547.6703567/1‑94 (MQ=60)
      GATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCCGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGC                                                                                     >  SRR4302547.2662684/1‑94 (MQ=60)
        TAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCC                                                                                   >  SRR4302547.2515211/1‑94 (MQ=60)
        TAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCC                                                                                   <  SRR4302547.453687/94‑1 (MQ=60)
         AATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCG                                                                                  <  SRR4302547.375979/94‑1 (MQ=60)
           TGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTGCGCCCGTA                                                                                >  SRR4302547.1317345/1‑94 (MQ=60)
           TGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTA                                                                                >  SRR4302547.2591952/1‑94 (MQ=60)
             CAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACG                                                                              <  SRR4302547.3244575/94‑1 (MQ=60)
             CAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACG                                                                              >  SRR4302547.3263017/1‑94 (MQ=60)
             CAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACG                                                                              >  SRR4302547.4431204/1‑94 (MQ=60)
               GGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCG                                                                            <  SRR4302547.894337/94‑1 (MQ=60)
               GGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCG                                                                            >  SRR4302547.1264218/1‑94 (MQ=60)
               GGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCG                                                                            <  SRR4302547.4513901/94‑1 (MQ=60)
                 ccaaagctgagatgacgggcgagccaccatgcctCCAGATAGCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCT                                                                          <  SRR4302547.2017121/60‑1 (MQ=60)
                 CCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCT                                                                          >  SRR4302547.1266445/1‑94 (MQ=60)
                 CCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCT                                                                          >  SRR4302547.5561807/1‑94 (MQ=60)
                 CCACGGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCG                                                                          <  SRR4302547.5347984/94‑1 (MQ=60)
                  cctcGCCGGTCTGGCGTGCCTGCCAGCTCGCCCCTAGATACCGCGGACCTGCCGGGTTTTCGAGAAAACGCCCCAGCTCCGCCCGTACGCGCTC                                                                         <  SRR4302547.220465/90‑1 (MQ=46)
                  CACTGCGGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTC                                                                         <  SRR4302547.4725049/94‑1 (MQ=60)
                  CACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTC                                                                         <  SRR4302547.3796534/94‑1 (MQ=60)
                  CACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTC                                                                         >  SRR4302547.1955068/1‑94 (MQ=60)
                  CACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTC                                                                         >  SRR4302547.125205/1‑94 (MQ=60)
                  CACGGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTC                                                                         <  SRR4302547.1461628/94‑1 (MQ=60)
                   ACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCG                                                                        >  SRR4302547.2876552/1‑94 (MQ=60)
                    CTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGG                                                                       >  SRR4302547.6396565/1‑94 (MQ=60)
                      GCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCC                                                                     <  SRR4302547.481326/94‑1 (MQ=60)
                       CCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCT                                                                    <  SRR4302547.6697385/94‑1 (MQ=60)
                       CCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGATTTCGAGAAAACGCGCCAGCTCCGCCCGTACCCGCTCGGCCT                                                                    >  SRR4302547.2650430/1‑94 (MQ=60)
                        CGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTG                                                                   >  SRR4302547.4483606/1‑94 (MQ=60)
                          GTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGG                                                                 >  SRR4302547.4639147/1‑94 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGC                                                                >  SRR4302547.5578837/1‑94 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGA                                                                >  SRR4302547.3571512/1‑94 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGA                                                                >  SRR4302547.5354367/1‑94 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGA                                                                >  SRR4302547.6516565/1‑94 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGA                                                                >  SRR4302547.3817503/1‑94 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGA                                                                <  SRR4302547.203058/94‑1 (MQ=60)
                           TATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACCCGCTCGGCCcgggg                                                                >  SRR4302547.6027587/1‑89 (MQ=60)
                            ATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGAC                                                               <  SRR4302547.5890528/94‑1 (MQ=60)
                            ATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGAC                                                               >  SRR4302547.5774660/1‑94 (MQ=60)
                            ATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGAC                                                               <  SRR4302547.1443890/94‑1 (MQ=60)
                             TGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACT                                                              <  SRR4302547.240597/94‑1 (MQ=60)
                             TGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACT                                                              >  SRR4302547.2142651/1‑94 (MQ=60)
                              GGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTC                                                             >  SRR4302547.2544390/1‑94 (MQ=60)
                              GGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTC                                                             <  SRR4302547.6325212/94‑1 (MQ=60)
                              GGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTC                                                             <  SRR4302547.1168748/94‑1 (MQ=60)
                              GCCCTCCCTGCCAGCTCGCCGCCAGATAACGCGTATCTGCGGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTC                                                             <  SRR4302547.3485077/94‑1 (MQ=60)
                               GCTTGCCTCCCTGCTCGCCGCCAGATACCGCGGATCTGCCGGTTTTTCGAGAAAACGCGCCAGCTCCGTCCGTACGCGCTCGGCCTGGGACTCA                                                            <  SRR4302547.4305233/94‑1 (MQ=60)
                               GCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCA                                                            <  SRR4302547.6001987/94‑1 (MQ=60)
                                ccgcCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCAT                                                           <  SRR4302547.2353247/90‑1 (MQ=60)
                                 CTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATC                                                          <  SRR4302547.3040848/94‑1 (MQ=60)
                                  TGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCC                                                         <  SRR4302547.599288/94‑1 (MQ=60)
                                  TGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCC                                                         <  SRR4302547.2798037/94‑1 (MQ=60)
                                  GGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCC                                                         <  SRR4302547.1828112/94‑1 (MQ=60)
                                   GCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCG                                                        <  SRR4302547.1742884/94‑1 (MQ=60)
                                    CCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGC                                                       <  SRR4302547.3170975/94‑1 (MQ=60)
                                    CCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGC                                                       >  SRR4302547.637098/1‑94 (MQ=60)
                                    CCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGC                                                       <  SRR4302547.2572985/94‑1 (MQ=60)
                                    CCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGC                                                       >  SRR4302547.5256647/1‑94 (MQ=60)
                                     CTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCC                                                      <  SRR4302547.5031725/94‑1 (MQ=60)
                                     CTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCC                                                      >  SRR4302547.306111/1‑94 (MQ=60)
                                     CTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCC                                                      >  SRR4302547.2540309/1‑94 (MQ=60)
                                     CTGCCACCTCGCCGCCAGATAGCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCC                                                      <  SRR4302547.2840801/94‑1 (MQ=60)
                                      TGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCA                                                     <  SRR4302547.1792041/94‑1 (MQ=60)
                                      TGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCA                                                     <  SRR4302547.3269435/94‑1 (MQ=60)
                                        CCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGC                                                   >  SRR4302547.2909816/1‑94 (MQ=60)
                                         CAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCC                                                  >  SRR4302547.837509/1‑94 (MQ=60)
                                         CAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCC                                                  >  SRR4302547.1626095/1‑94 (MQ=60)
                                          AGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCA                                                 >  SRR4302547.386689/1‑94 (MQ=60)
                                          AGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCA                                                 >  SRR4302547.2343377/1‑94 (MQ=60)
                                             TCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGAC                                              >  SRR4302547.5583308/1‑94 (MQ=60)
                                             TCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGAC                                              <  SRR4302547.1121501/94‑1 (MQ=60)
                                               GCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAT                                            >  SRR4302547.992603/1‑94 (MQ=60)
                                                CCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATC                                           >  SRR4302547.1942421/1‑94 (MQ=60)
                                                CCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATC                                           >  SRR4302547.881731/1‑94 (MQ=60)
                                                CCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATC                                           <  SRR4302547.575347/94‑1 (MQ=60)
                                                 CGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCG                                          <  SRR4302547.1535607/94‑1 (MQ=60)
                                                 CGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCG                                          >  SRR4302547.6481024/1‑94 (MQ=60)
                                                 CGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCG                                          <  SRR4302547.107482/94‑1 (MQ=60)
                                                 CGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCG                                          <  SRR4302547.4910484/94‑1 (MQ=60)
                                                   CCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTCCGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCT                                        <  SRR4302547.1609456/94‑1 (MQ=60)
                                                    CAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTT                                       >  SRR4302547.4419605/1‑94 (MQ=60)
                                                    CAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTT                                       <  SRR4302547.6733923/94‑1 (MQ=60)
                                                     AGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTT                                      >  SRR4302547.4360600/1‑94 (MQ=60)
                                                     AGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGAAATCGCTTT                                      >  SRR4302547.2509700/1‑94 (MQ=60)
                                                      GATAGCGCGGATCTGCCGGGTTTTCGAGAAACCGCCCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTG                                     <  SRR4302547.5018094/94‑1 (MQ=60)
                                                       ATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGG                                    >  SRR4302547.1818097/1‑94 (MQ=60)
                                                        AAACGCGGTTCTGCCGTGTTTTCGAGAAAACGCGCCAGCTCGGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGT                                   <  SRR4302547.1192105/94‑1 (MQ=60)
                                                         AACGCGGATCTGCCGTGTTTTCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTT                                  <  SRR4302547.754344/94‑1 (MQ=60)
                                                         AACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCCGACATCGCTTTGGTT                                  >  SRR4302547.4839520/1‑94 (MQ=60)
                                                         AACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTT                                  >  SRR4302547.1518806/1‑94 (MQ=60)
                                                            GCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATG                               >  SRR4302547.4869720/1‑94 (MQ=60)
                                                             CGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGT                              <  SRR4302547.3402408/94‑1 (MQ=60)
                                                             CGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGT                              <  SRR4302547.2028221/94‑1 (MQ=60)
                                                             CGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGT                              >  SRR4302547.2608285/1‑94 (MQ=60)
                                                              GGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTT                             >  SRR4302547.819550/1‑94 (MQ=60)
                                                              GGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTT                             >  SRR4302547.5338282/1‑94 (MQ=60)
                                                              GGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTT                             <  SRR4302547.4892369/94‑1 (MQ=60)
                                                              GGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTT                             <  SRR4302547.4232621/94‑1 (MQ=60)
                                                              GGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTT                             <  SRR4302547.2654162/94‑1 (MQ=60)
                                                              GGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTT                             >  SRR4302547.5003303/1‑94 (MQ=60)
                                                               GATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTG                            <  SRR4302547.6685452/94‑1 (MQ=60)
                                                                  CTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTG                         <  SRR4302547.5203406/94‑1 (MQ=60)
                                                                   TGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGA                        <  SRR4302547.5485545/94‑1 (MQ=60)
                                                                   TGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGA                        <  SRR4302547.3349318/94‑1 (MQ=60)
                                                                    gctggttttcCGAGAAACCGCGCCAGCTCCGCCCGTACTCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAA                       <  SRR4302547.18352/84‑1 (MQ=60)
                                                                     cagggtttcaGAGAAACCGCGCCACATCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAT                      <  SRR4302547.5734965/84‑1 (MQ=60)
                                                                     CCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAT                      >  SRR4302547.3665523/1‑94 (MQ=60)
                                                                     CCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAT                      >  SRR4302547.725546/1‑94 (MQ=60)
                                                                     CCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAT                      >  SRR4302547.5941540/1‑94 (MQ=60)
                                                                      CGGGTTTTGGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATC                     <  SRR4302547.350473/94‑1 (MQ=60)
                                                                      CGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTAATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATC                     >  SRR4302547.633688/1‑94 (MQ=60)
                                                                       GGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCG                    >  SRR4302547.2897618/1‑94 (MQ=60)
                                                                       GGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCG                    >  SRR4302547.4745944/1‑94 (MQ=60)
                                                                       GGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCG                    <  SRR4302547.963558/94‑1 (MQ=60)
                                                                        GGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGT                   >  SRR4302547.6098663/1‑94 (MQ=60)
                                                                         GTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTG                  <  SRR4302547.3673284/94‑1 (MQ=60)
                                                                         GTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTG                  <  SRR4302547.5191186/94‑1 (MQ=60)
                                                                           TTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAG                <  SRR4302547.617414/94‑1 (MQ=60)
                                                                            TTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGG               <  SRR4302547.3627546/94‑1 (MQ=60)
                                                                              CGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAT             >  SRR4302547.4174910/1‑94 (MQ=60)
                                                                              CGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAT             <  SRR4302547.2353946/94‑1 (MQ=60)
                                                                              CGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAT             <  SRR4302547.2535746/94‑1 (MQ=60)
                                                                               GAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATA            >  SRR4302547.6465398/1‑94 (MQ=60)
                                                                               GAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATA            >  SRR4302547.644452/1‑94 (MQ=60)
                                                                               GAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATA            <  SRR4302547.2784037/94‑1 (MQ=60)
                                                                                  AAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACA         <  SRR4302547.102603/94‑1 (MQ=60)
                                                                                    ACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACC       <  SRR4302547.6399539/94‑1 (MQ=60)
                                                                                    AACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACC       >  SRR4302547.5864783/1‑94 (MQ=60)
                                                                                    AACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCCTTGGTTATGTTGTTGAATCGTGAGGATAACACC       <  SRR4302547.3976464/94‑1 (MQ=60)
                                                                                      CGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCT     <  SRR4302547.4784746/94‑1 (MQ=60)
                                                                                      CGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCT     <  SRR4302547.9464/94‑1 (MQ=60)
                                                                                        CGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGC   >  SRR4302547.5915108/1‑94 (MQ=60)
                                                                                         GCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCG  <  SRR4302547.4316765/94‑1 (MQ=60)
                                                                                             |                                                                                         
AACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCG  >  NC_000913/3558839‑3559021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑